1. Immunoproteomics analysis of food allergens.
- Author
-
Nakamura R and Teshima R
- Subjects
- Fluorescence, Humans, Immunoblotting, Isoelectric Focusing, Mass Spectrometry, Oryza metabolism, Plant Proteins chemistry, Allergens immunology, Food Hypersensitivity immunology, Plant Proteins immunology, Proteomics methods
- Abstract
Immunoblot-coupled proteomics based on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), namely, immunoproteomics, has been used for comprehensive identification of food allergens, because it is a simple and inexpensive tool for rapid identification of several IgE-binding proteins. In this section, we describe our protocols for identification of food allergens using immunoproteomics and discuss a few technical points in detail.
- Published
- 2014
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