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RNAi-mediated suppression of endogenous storage proteins leads to a change in localization of overexpressed cholera toxin B-subunit and the allergen protein RAG2 in rice seeds.
- Source :
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Plant cell reports [Plant Cell Rep] 2014 Jan; Vol. 33 (1), pp. 75-87. Date of Electronic Publication: 2013 Oct 02. - Publication Year :
- 2014
-
Abstract
- Key Message: RNAi-mediated suppression of the endogenous storage proteins in MucoRice-CTB-RNAi seeds affects not only the levels of overexpressed CTB and RAG2 allergen, but also the localization of CTB and RAG2. A purification-free rice-based oral cholera vaccine (MucoRice-CTB) was previously developed by our laboratories using a cholera toxin B-subunit (CTB) overexpression system. Recently, an advanced version of MucoRice-CTB was developed (MucoRice-CTB-RNAi) through the use of RNAi to suppress the production of the endogenous storage proteins 13-kDa prolamin and glutelin, so as to increase CTB expression. The level of the α-amylase/trypsin inhibitor-like protein RAG2 (a major rice allergen) was reduced in MucoRice-CTB-RNAi seeds in comparison with wild-type (WT) rice. To investigate whether RNAi-mediated suppression of storage proteins affects the localization of overexpressed CTB and major rice allergens, we generated an RNAi line without CTB (MucoRice-RNAi) and investigated gene expression, and protein production and localization of two storage proteins, CTB, and five major allergens in MucoRice-CTB, MucoRice-CTB-RNAi, MucoRice-RNAi, and WT rice. In all lines, glyoxalase I was detected in the cytoplasm, and 52- and 63-kDa globulin-like proteins were found in the aleurone particles. In WT, RAG2 and 19-kDa globulin were localized mainly in protein bodies II (PB-II) of the endosperm cells. Knockdown of glutelin A led to a partial destruction of PB-II and was accompanied by RAG2 relocation to the plasma membrane/cell wall and cytoplasm. In MucoRice-CTB, CTB was localized in the cytoplasm and PB-II. In MucoRice-CTB-RNAi, CTB was produced at a level six times that in MucoRice-CTB and was localized, similar to RAG2, in the plasma membrane/cell wall and cytoplasm. Our findings indicate that the relocation of CTB in MucoRice-CTB-RNAi may contribute to down-regulation of RAG2.
- Subjects :
- Allergens ultrastructure
Electrophoresis, Polyacrylamide Gel
Fluorescent Antibody Technique
Gene Expression Regulation, Plant
Glutens metabolism
Oryza genetics
Oryza ultrastructure
Plants, Genetically Modified
RNA, Messenger genetics
RNA, Messenger metabolism
Seeds genetics
Seeds ultrastructure
Allergens metabolism
Cholera Toxin metabolism
Oryza metabolism
RNA Interference
Seed Storage Proteins metabolism
Seeds metabolism
Subjects
Details
- Language :
- English
- ISSN :
- 1432-203X
- Volume :
- 33
- Issue :
- 1
- Database :
- MEDLINE
- Journal :
- Plant cell reports
- Publication Type :
- Academic Journal
- Accession number :
- 24085308
- Full Text :
- https://doi.org/10.1007/s00299-013-1513-3