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Immunoproteomics analysis of food allergens.
- Source :
-
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2014; Vol. 1072, pp. 725-35. - Publication Year :
- 2014
-
Abstract
- Immunoblot-coupled proteomics based on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), namely, immunoproteomics, has been used for comprehensive identification of food allergens, because it is a simple and inexpensive tool for rapid identification of several IgE-binding proteins. In this section, we describe our protocols for identification of food allergens using immunoproteomics and discuss a few technical points in detail.
Details
- Language :
- English
- ISSN :
- 1940-6029
- Volume :
- 1072
- Database :
- MEDLINE
- Journal :
- Methods in molecular biology (Clifton, N.J.)
- Publication Type :
- Academic Journal
- Accession number :
- 24136559
- Full Text :
- https://doi.org/10.1007/978-1-62703-631-3_50