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Immunoproteomics analysis of food allergens.

Authors :
Nakamura R
Teshima R
Source :
Methods in molecular biology (Clifton, N.J.) [Methods Mol Biol] 2014; Vol. 1072, pp. 725-35.
Publication Year :
2014

Abstract

Immunoblot-coupled proteomics based on two-dimensional polyacrylamide gel electrophoresis (2D-PAGE), namely, immunoproteomics, has been used for comprehensive identification of food allergens, because it is a simple and inexpensive tool for rapid identification of several IgE-binding proteins. In this section, we describe our protocols for identification of food allergens using immunoproteomics and discuss a few technical points in detail.

Subjects

Subjects :
Fluorescence
Humans
Immunoblotting
Isoelectric Focusing
Mass Spectrometry
Oryza metabolism
Plant Proteins chemistry
Allergens immunology
Food Hypersensitivity immunology
Plant Proteins immunology
Proteomics methods

Details

Language :
English
ISSN :
1940-6029
Volume :
1072
Database :
MEDLINE
Journal :
Methods in molecular biology (Clifton, N.J.)
Publication Type :
Academic Journal
Accession number :
24136559
Full Text :
https://doi.org/10.1007/978-1-62703-631-3_50
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