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Allergenicity study of EGFP-transgenic chicken meat by serological and 2D-DIGE analysis.
- Source :
-
Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association [Food Chem Toxicol] 2010 May; Vol. 48 (5), pp. 1302-10. Date of Electronic Publication: 2010 Feb 25. - Publication Year :
- 2010
-
Abstract
- Genetically modified (GM) foods must be tested for safety, including by allergenicity tests to ensure that they do not contain new allergens or higher concentrations of known allergens than the same non-GM foods. In this study experimentally developed EGFP-transgenic chickens were used and evaluated the allergenicity of meat from the chicken based on a serological and two-dimensional difference gel electrophoresis (2D-DIGE) analysis. For the serological analysis, a Western blotting with allergen-specific antibodies and a proteomic analysis of chicken meat allergens with patients' sera, a so-called allergenome analysis, were used. The allergenome analysis allowed us to identify five IgE-binding proteins in chicken meat, including a known allergen, chicken serum albumin, and no qualitative difference in their expressions between the GM and non-GM chicken meat was found. Results of the 2D-DIGE analysis showed that none of the IgE-binding proteins in chicken meat were significantly changed in expression levels between non-GM and GM chicken, and only 3 of the 1500 soluble protein spots including green fluorescence protein were markedly different as a result of gene transfer. These above results showed that the combination of serological and 2D-DIGE analysis is a valid method of evaluating quality and quantity of allergens in GM foods.<br /> (Copyright (c) 2010 Elsevier Ltd. All rights reserved.)
- Subjects :
- Allergens immunology
Animals
Chickens
Electrophoresis, Gel, Two-Dimensional
Food Hypersensitivity blood
Food Hypersensitivity etiology
Gene Expression Profiling
Green Fluorescent Proteins biosynthesis
Green Fluorescent Proteins genetics
Humans
Immunoglobulin E analysis
Immunoglobulin E metabolism
Muscle Proteins immunology
Peptide Mapping
Proteomics
Serum Albumin analysis
Serum Albumin immunology
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Tissue Extracts immunology
Allergens adverse effects
Animals, Genetically Modified immunology
Food Hypersensitivity immunology
Food, Genetically Modified adverse effects
Meat adverse effects
Tissue Extracts adverse effects
Subjects
Details
- Language :
- English
- ISSN :
- 1873-6351
- Volume :
- 48
- Issue :
- 5
- Database :
- MEDLINE
- Journal :
- Food and chemical toxicology : an international journal published for the British Industrial Biological Research Association
- Publication Type :
- Academic Journal
- Accession number :
- 20188780
- Full Text :
- https://doi.org/10.1016/j.fct.2010.02.028