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Comparative study of GH-transgenic and non-transgenic amago salmon (Oncorhynchus masou ishikawae) allergenicity and proteomic analysis of amago salmon allergens.
- Source :
-
Regulatory toxicology and pharmacology : RTP [Regul Toxicol Pharmacol] 2009 Dec; Vol. 55 (3), pp. 300-8. Date of Electronic Publication: 2009 Aug 11. - Publication Year :
- 2009
-
Abstract
- Genetically modified (GM) foods are beneficial from the standpoint of ensuring a constant supply of foodstuffs, but they must be tested for safety before being released on the market, including by allergenicity tests to ensure that they do not contain new allergens or higher concentrations of known allergens than the same non-GM foods. In this study we used GM-amago salmon into which a growth hormone gene had been introduced and compared the allergens contained in the GM and the non-GM-amago salmons. We used a combination of Western blotting with allergen-specific antibodies and a proteomic analysis of their allergens with patients' sera, a so-called allergenome analysis, to analyze allergens. Western blotting with specific antibodies showed no increase in the content of the known allergens fish parvalbumin and fish type-I collagen in GM-amago salmon, in comparison with their content in non-GM-amago salmon. The allergenome analysis of two fish-allergic patients allowed us to identify several IgE-binding proteins in amago salmon, including parvalbumin, triose-phosphate isomerase, fructose-bisphosphate aldolase A, and serum albumin, and there were no qualitative differences in these proteins between GM and non-GM-amago salmons. These results indicate that amago salmon endogenous allergen expression does not seem to be altered by genetic modification.
- Subjects :
- Allergens analysis
Animals
Animals, Genetically Modified immunology
Blotting, Western
Collagen Type I immunology
Growth Hormone genetics
Humans
Parvalbumins immunology
Proteomics methods
Salmon genetics
Allergens immunology
Food Hypersensitivity immunology
Food, Genetically Modified toxicity
Salmon immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0295
- Volume :
- 55
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Regulatory toxicology and pharmacology : RTP
- Publication Type :
- Academic Journal
- Accession number :
- 19679156
- Full Text :
- https://doi.org/10.1016/j.yrtph.2009.08.002