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Evaluation of allergenicity of acid-hydrolyzed wheat protein using an in vitro elicitation test.
- Source :
-
International archives of allergy and immunology [Int Arch Allergy Immunol] 2013; Vol. 160 (3), pp. 259-64. Date of Electronic Publication: 2012 Oct 17. - Publication Year :
- 2013
-
Abstract
- Background: We performed an in vitro elicitation test to determine the ability of different types of wheat-allergic patients' IgE to induce humanized mast cell activation after the addition of various time-treated acid-hydrolyzed wheat proteins (HWPs).<br />Methods: The reactivity of heat- and various time-treated acid-hydrolyzed glutens (acid-HGs) and commercial acid-HWP (HWP1), using serum IgE from wheat allergy accompanied by skin and rhinoconjunctival sensitization to HWP1 in the facial soap, pediatric subjects with food allergy to native wheat, adult wheat-dependent exercise-induced anaphylaxis subjects, and nonatopic healthy subjects, was elucidated by dot blot and a luciferase assay-based in vitro elicitation test (EXiLE test).<br />Results: Serum from subjects sensitized with HWP1 reacted only to acid-HGs (acid-HGs treated for 0.5-3 or 6 h), but not native gluten, in the results of the dot blot. In contrast, sera from pediatric subjects sensitized with native wheat reacted to native gluten more strongly and showed only slight reactions to 0.5- to 1-hour-treated acid-HGs. The results of the in vitro elicitation test showed that acid hydrolyzation of the gluten attenuated antigen-induced luciferase expression in a time-dependent manner for sera from native-wheat-sensitized pediatric subjects. On the other hand, in the sera from HWP1-sensitized subjects, acid hydrolyzation of the gluten for 0.5 h dramatically increased luciferase expression.<br />Conclusions: Even after prolonged hydrolyzation, acid-HGs still retained the ability to activate mast cells in the case of HWP1-sensitized subjects.<br /> (Copyright © 2012 S. Karger AG, Basel.)
- Subjects :
- Acids chemistry
Adolescent
Adult
Aged
Allergens adverse effects
Allergens chemistry
Anaphylaxis complications
Asthma, Exercise-Induced complications
Cell Degranulation
Cells, Cultured
Child
Child, Preschool
Conjunctivitis, Allergic complications
Female
Glutens chemistry
Humans
Hydrolysis
Immunoglobulin E metabolism
Infant
Male
Middle Aged
Plant Proteins adverse effects
Plant Proteins chemistry
Protein Binding
Rhinitis, Allergic, Perennial complications
Triticum adverse effects
Triticum immunology
Wheat Hypersensitivity complications
Young Adult
Allergens immunology
Anaphylaxis immunology
Asthma, Exercise-Induced immunology
Conjunctivitis, Allergic immunology
Glutens immunology
Mast Cells immunology
Plant Proteins immunology
Rhinitis, Allergic, Perennial immunology
Skin immunology
Wheat Hypersensitivity immunology
Subjects
Details
- Language :
- English
- ISSN :
- 1423-0097
- Volume :
- 160
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- International archives of allergy and immunology
- Publication Type :
- Academic Journal
- Accession number :
- 23075478
- Full Text :
- https://doi.org/10.1159/000341671