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Proteomic analysis of known and candidate rice allergens between non-transgenic and transgenic plants.
- Source :
-
Regulatory toxicology and pharmacology : RTP [Regul Toxicol Pharmacol] 2011 Apr; Vol. 59 (3), pp. 437-44. Date of Electronic Publication: 2011 Feb 05. - Publication Year :
- 2011
-
Abstract
- Salt-soluble proteins extracted from non-transgenic and transgenic rice were evaluated for the presence of known and potential allergens by proteomic techniques. The salt-soluble proteins were extracted, separated by 1D and 2D electrophoresis, and analyzed by Western blotting. 1D immunoblot analysis with patients' sera revealed few qualitative differences between the IgE-binding proteins of the non-transgenic and transgenic rice. 1D immunoblot with antigen-specific-animal sera revealed no qualitative or quantitative differences in two known allergens, RAG2 and glyoxalase I, between non-transgenic and transgenic rice. Multiple spots containing known and novel IgE-binding proteins were detected among the salt-soluble proteins of non-transgenic rice by 2D immunoblotting. Two globulin-like proteins, a 52 kDa protein and a 63 kDa protein, were identified as novel IgE-binding proteins that are candidates for rice allergens. These globulin-like proteins were homologous to Cupin superfamily allergens. Quantitative analysis of 19, 52, and 63 kDa globulins with protein-specific-animal sera showed no significant differences in the expression of these proteins between the transgenic rice and non-transgenic rice. These results indicate that none of the known or novel endogenous IgE-binding proteins detected in this study appear to be altered by genetic modification.<br /> (Copyright © 2011 Elsevier Inc. All rights reserved.)
- Subjects :
- Allergens genetics
Allergens metabolism
Animals
Binding Sites, Antibody
Humans
Immunoglobulin E metabolism
Oryza genetics
Plant Proteins genetics
Plant Proteins metabolism
Plants, Genetically Modified genetics
Seeds genetics
Seeds immunology
Allergens immunology
Oryza immunology
Plant Proteins immunology
Plants, Genetically Modified immunology
Proteomics methods
Subjects
Details
- Language :
- English
- ISSN :
- 1096-0295
- Volume :
- 59
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Regulatory toxicology and pharmacology : RTP
- Publication Type :
- Academic Journal
- Accession number :
- 21300107
- Full Text :
- https://doi.org/10.1016/j.yrtph.2011.01.008