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Evaluation of the luciferase assay-based in vitro elicitation test for serum IgE.
- Source :
-
Allergology international : official journal of the Japanese Society of Allergology [Allergol Int] 2012 Sep; Vol. 61 (3), pp. 431-7. - Publication Year :
- 2012
-
Abstract
- Background: An in vitro elicitation test employing human high-affinity IgE receptor-expressing rat mast cell lines appears to be a useful method for measuring mast cell activation using a patient's IgE and an allergen; however, such cell lines are sensitive to human complements in the serum. We have recently developed a new luciferase-reporting mast cell line (RS-ATL8) to detect IgE crosslinking-induced luciferase expression (EXiLE) with relatively low quantities of serum IgE.<br />Methods: A total of 30 patients suspected of having egg white (EW) allergy were subjected to an oral food challenge (OFC) test; then, the performances of EW-specific serum IgE (CAP-FEIA), EW-induced degranulation, and EXiLE responses in RS-ATL8 cells were compared using receiver-operating characteristic (ROC) curve analysis. The patients' sera were diluted to 1 : 100, which causes no cytotoxicity when sensitizing the RS-ATL8 cells for the degranulation and EXiLE tests.<br />Results: The area under the ROC curves was highest in the EXiLE test (0.977), followed by CAP-FEIA (0.926) and degranulation (0.810). At an optimal cutoff range (1.648-1.876) calculated from the ROC curve of the EXiLE test, sensitivity and specificity were 0.944 and 0.917, respectively. A 95% positive predictive value was given at a cutoff level of 2.054 (fold increase in luciferase expression) by logistic regression analysis.<br />Conclusions: In contrast to in vivo tests, the EXiLE test appears to be a useful tool in diagnosing patients suspected of having IgE-dependent EW allergy without the risk of severe systemic reactions.
- Subjects :
- Adolescent
Adult
Animals
Antibody Specificity immunology
Cell Degranulation
Cell Line
Child
Child, Preschool
Female
Humans
Immunoenzyme Techniques
Immunoglobulin E immunology
Luminescent Measurements
Male
Mast Cells immunology
Mast Cells metabolism
ROC Curve
Rats
Sensitivity and Specificity
Young Adult
Allergens immunology
Egg Hypersensitivity diagnosis
Egg Hypersensitivity immunology
Egg White adverse effects
Immunoglobulin E blood
Luciferases
Subjects
Details
- Language :
- English
- ISSN :
- 1440-1592
- Volume :
- 61
- Issue :
- 3
- Database :
- MEDLINE
- Journal :
- Allergology international : official journal of the Japanese Society of Allergology
- Publication Type :
- Academic Journal
- Accession number :
- 22722812
- Full Text :
- https://doi.org/10.2332/allergolint.11-OA-0407