1. Fungal Coculture of Herpotrichia sp. and Trametes versicolor Induces Production of Diverse Metabolites with Anti-Parkinson's Neuroprotective Activity.
- Author
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Wang ZJ, Hong YR, Wang XY, Wang JZ, Zhai YJ, Cui W, and Han WB
- Subjects
- Animals, PC12 Cells, Molecular Structure, Rats, Sesquiterpenes pharmacology, Sesquiterpenes chemistry, Parkinson Disease drug therapy, Parkinson Disease metabolism, Polyporaceae chemistry, Coculture Techniques, Neuroprotective Agents pharmacology, Neuroprotective Agents chemistry
- Abstract
Co-cultivation of isopod-associated fungi Herpotrichia sp. SF09 and Trametes versicolor SF09A led to the reciprocal induction of thirteen new compounds ( 1 - 7 and 9 - 13 ) with diverse architectures. Importantly, compounds 1 and 2 are rare fungal sesquiterpene-saccharide hybrids incorporating a xylopyranose moiety, compound (±)- 3 represents the first example of a natural linear sesquiterpene racemate, and compound 7 is a rare α-pyrone derivative with a xylopyranose motif. Their structures were elucidated by analysis of NMR and mass spectrometry data, and their absolute configurations were determined by Mosher's method, microscale derivatization, and single-crystal X-ray diffraction, as well as ECD calculations. All the isolated compounds ameliorated MPP
+ -induced oxidative damage in PC12 cells in a dose-dependent fashion. Among them, compounds 5 and 15 showed significant protective action against neuronal injury by MPP+ at 5 μM. Meanwhile, transcriptome sequencing was performed to evaluate the molecular mechanism of the neuroprotective activity for compound 5 . Results indicated that compound 5 might mitigate MPP+ -induced neuronal injury through the regulation of multiple signaling pathways, including the PI3K-Akt and MAPK pathways. Our findings suggested that compound 5 could be a promising neuroprotective agent for the treatment of Parkinson's disease.- Published
- 2024
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