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Amauroderma rugosum Protects PC12 Cells against 6-OHDA-Induced Neurotoxicity through Antioxidant and Antiapoptotic Effects.
- Source :
-
Oxidative medicine and cellular longevity [Oxid Med Cell Longev] 2021 Feb 03; Vol. 2021, pp. 6683270. Date of Electronic Publication: 2021 Feb 03 (Print Publication: 2021). - Publication Year :
- 2021
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Abstract
- Amauroderma rugosum (AR) is a dietary mushroom in the Ganodermataceae family whose pharmacological activity and medicinal value have rarely been reported. In this study, the antioxidant capacity and neuroprotective effects of AR were investigated. The aqueous extract of AR was confirmed to contain phenolic compounds, polysaccharides, and triterpenes. The results of 2,2-diphenyl-1-picryl-hydrazyl-hydrate (DPPH) and total antioxidant capacity assays revealed that AR extract scavenged reactive oxygen species. Moreover, AR extract decreased the cytotoxicity, oxidative stress, mitochondrial dysfunction, and apoptosis of PC12 cells induced by 6-hydroxydopamine (6-OHDA). In addition, 6-OHDA upregulated the expressions of proapoptotic proteins and downregulated the Akt (protein kinase B)/mTOR- (mammalian target of rapamycin-) and MEK (mitogen-activated protein kinase kinase)/ERK- (extracellular signal-regulated kinases-) dependent signaling pathways. These effects of 6-OHDA were abolished or partially reversed by AR extract. Furthermore, the neuroprotective effects of AR in 6-OHDA-treated PC12 cells were significantly abolished by Akt and MEK inhibitor. Thus, AR extract possesses neuroprotective effects, probably through its antioxidant and antiapoptotic effects. These findings suggest the potential application of AR in the prevention or treatment of oxidative stress-related neurodegenerative diseases such as Parkinson's disease.<br />Competing Interests: The authors declare no conflict of interest.<br /> (Copyright © 2021 Jingjing Li et al.)
- Subjects :
- Animals
Biphenyl Compounds chemistry
Cell Death drug effects
Cell Respiration drug effects
MAP Kinase Signaling System drug effects
Membrane Potential, Mitochondrial drug effects
Mitochondria drug effects
Mitochondria metabolism
Mitogen-Activated Protein Kinase Kinases antagonists & inhibitors
Mitogen-Activated Protein Kinase Kinases metabolism
Oxidopamine
PC12 Cells
Picrates chemistry
Proto-Oncogene Proteins c-akt antagonists & inhibitors
Proto-Oncogene Proteins c-akt metabolism
Rats
Reactive Oxygen Species metabolism
TOR Serine-Threonine Kinases metabolism
Antioxidants pharmacology
Apoptosis drug effects
Neuroprotective Agents pharmacology
Neurotoxins toxicity
Polyporaceae chemistry
Subjects
Details
- Language :
- English
- ISSN :
- 1942-0994
- Volume :
- 2021
- Database :
- MEDLINE
- Journal :
- Oxidative medicine and cellular longevity
- Publication Type :
- Academic Journal
- Accession number :
- 33628381
- Full Text :
- https://doi.org/10.1155/2021/6683270