190 results on '"GUO Dong-liang"'
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2. The Necessary Condition of Families of Odd-Periodic Perfect Complementary Sequence Pairs.
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Hui-long Jin, Guo-dong Liang, Zhi-Hua Liu, and Cheng-qian Xu
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- 2009
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3. From discovery to outbreak: the genetic evolution of the emerging Zika virus
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Hong Liu, Liang Shen, Xiao-Lin Zhang, Xiao-Long Li, Guo-Dong Liang, and Hong-Fang Ji
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Infectious and parasitic diseases ,RC109-216 ,Microbiology ,QR1-502 - Published
- 2016
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4. Human Infection with West Nile Virus, Xinjiang, China, 2011
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Zhi Lu, Shi-Hong Fu, Lei Cao, Cheng-Jun Tang, Song Zhang, Zhao-Xia Li, Mamutijiang Tusong, Xin-Hua Yao, Hai-Lin Zhang, Pi-Yu Wang, Maimaitijiang Wumaier, Xue-Yan Yuan, Ming-Hua Li, Chang-Zhong Zhu, Li-Ping Fu, and Guo-Dong Liang
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West Nile virus ,viruses ,encephalitis ,China ,human infection ,Xinjiang ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Published
- 2014
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5. Banna Virus, China, 1987–2007
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Hong Liu, Ming-Hua Li, You-Gang Zhai, Wei-Shan Meng, Xiao-Hong Sun, Yu-Xi Cao, Shi-Hong Fu, Huan-Yu Wang, Li-Hong Xu, Qing Tang, and Guo-Dong Liang
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Banna virus ,distribution ,phylogeny ,China ,viruses ,dispatch ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
Banna viruses (BAVs) have been isolated from pigs, cattle, ticks, mosquitoes, and human encephalitis patients. We isolated and analyzed 20 BAVs newly isolated in China; this finding extends the distribution of BAVs from tropical zone to north temperate climates and demonstrate regional variations in BAV phylogeny and mosquito species possibly involved in BAV transmission.
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- 2010
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6. Molecular Epidemiology of Rabies in Southern People’s Republic of China
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Xiao-Yan Tao, Qing Tang, Hao Li, Zhao-Jun Mo, Hong Zhang, Ding-Ming Wang, Qiang Zhang, Miao Song, Andres Velasco-Villa, Xianfu Wu, Charles E. Rupprecht, and Guo-Dong Liang
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Molecular epidemiology ,nucleoprotein ,rabies ,epidemic ,China ,viruses ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
In recent years, the number of human rabies cases in the People’s Republic of China has increased during severe epidemics in 3 southern provinces (Guizhou, Guangxi, and Hunan). To analyze the causes of the high incidence of human rabies in this region, during 2005–2007, we collected 2,887 brain specimens from apparently healthy domestic dogs used for meat consumption in restaurants, 4 specimens from suspected rabid dogs, and 3 from humans with rabies in the 3 provinces. Partial nucleoprotein gene sequences were obtained from rabies-positive specimens. Phylogenetic relationships and distribution of viruses were determined. We infer that the spread of rabies viruses from high-incidence regions, particularly by long-distance movement or transprovincial translocation of dogs caused by human-related activities, may be 1 cause of the recent massive human rabies epidemics in southern China.
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- 2009
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7. Japanese Encephalitis Viruses from Bats in Yunnan, China
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Jing-Lin Wang, Xiao-Ling Pan, Hai-Lin Zhang, Shi-Hong Fu, Huan-Yu Wang, Qing Tang, Lin-Fa Wang, and Guo-Dong Liang
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Japanese encephalitis ,bats ,epidemiology ,phylogeny ,China ,viruses ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
Genome sequencing and virulence studies of 2 Japanese encephalitis viruses (JEVs) from bats in Yunnan, China, showed a close relationship with JEVs isolated from mosquitoes and humans in the same region over 2 decades. These results indicate that bats may play a role in human Japanese encephalitis outbreaks in this region.
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- 2009
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8. Tahyna Virus and Human Infection, China
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Zhi Lu, Xin-Jun Lu, Shi-Hong Fu, Song Zhang, Zhao-Xia Li, Xin-Hua Yao, Yu-Ping Feng, Amy J. Lambert, Da-Xin Ni, Feng-Tian Wang, Su-Xiang Tong, Roger S. Nasci, Yun Feng, Qiang Dong, You-Gang Zhai, Xiao-Yan Gao, Huan-Yu Wang, Qing Tang, and Guo-Dong Liang
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Tahyna virus ,California group viruses ,Bunyaviridae ,China ,dispatch ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
In 2006, Tahyna virus was isolated from Culex spp. mosquitoes collected in Xinjiang, People’s Republic of China. In 2007, to determine whether this virus was infecting humans, we tested serum from febrile patients. We found immunoglobulin (Ig) M and IgG against the virus, which suggests human infection in this region.
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- 2009
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9. Japanese Encephalitis, Tibet, China
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Yi-Xing Li, Ming-Hua Li, Shi-Hong Fu, Wei-Xin Chen, Qi-Yong Liu, Hai-Lin Zhang, Wa Da, Song-Lin Hu, Sang Dan La Mu, Ju Bai, Zun-Dong Yin, Hong-Yue Jiang, Yu-Hong Guo, Dun Zhu Duo Ji, Hui-Mei Xu, Ge Li, Gu Gu Cuo Mu, Hui-Ming Luo, Jing-Lin Wang, Jun Wang, Xiu-Min Ye, Zhuo Ma Yang Jin, Wei Zhang, Gui-Jun Ning, Huan-Yu Wang, Gui-Chang Li, Jian Yong, Xiao-Feng Liang, and Guo-Dong Liang
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Japanese encephalitis virus ,isolation ,identification ,mosquitoes ,viruses ,vector-borne infections ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Published
- 2011
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10. Isolation of Kyasanur Forest Disease Virus from Febrile Patient, Yunnan, China
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Jinglin Wang, Hailin Zhang, Shihong Fu, Huanyu Wang, Daxin Ni, Roger S. Nasci, Qing Tang, and Guo-Dong Liang
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Kyasanur Forest disease virus (KFDV) ,Alkhurma virus ,Nanjianyin virus ,China ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
We recently determined that Nanjianyin virus, isolated from serum of a patient in Yunnan Province, China, in 1989, is a type of Kyasanur Forest disease virus. Results of a 1987–1990 seroepidemiologic investigation in Yunnan Province had shown that residents of the Hengduan Mountain region had been infected with Nanjianyin virus.
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- 2009
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11. Duration of Antibody Responses after Severe Acute Respiratory Syndrome
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Li-Ping Wu, Nai-Chang Wang, Yi-Hua Chang, Xiang-Yi Tian, Dan-Yu Na, Li-Yuan Zhang, Lei Zheng, Tao Lan, Lin-Fa Wang, and Guo-Dong Liang
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SARS ,convalescent ,antibodies ,longitudinal study ,dispatch ,China ,Medicine ,Infectious and parasitic diseases ,RC109-216 - Abstract
Among 176 patients who had had severe acute respiratory syndrome (SARS), SARS-specific antibodies were maintained for an average of 2 years, and significant reduction of immunoglobulin G–positive percentage and titers occurred in the third year. Thus, SARS patients might be susceptible to reinfection >3 years after initial exposure.
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- 2007
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12. Molecular phylodynamic analysis indicates lineage displacement occurred in Chinese rabies epidemics between 1949 to 2010.
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Xiao-Yan Tao, Qing Tang, Simon Rayner, Zhen-Yang Guo, Hao Li, Shu-Lin Lang, Cui-Ping Yin, Na Han, Wei Fang, James Adams, Miao Song, and Guo-Dong Liang
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Arctic medicine. Tropical medicine ,RC955-962 ,Public aspects of medicine ,RA1-1270 - Abstract
Rabies remains a serious problem in China with three epidemics since 1949 and the country in the midst of the third epidemic. Significantly, the control of each outbreak has been followed by a rapid reemergence of the disease. In 2005, the government implemented a rabies national surveillance program that included the collection and screening of almost 8,000 samples. In this work, we analyzed a Chinese dataset comprising 320 glycoprotein sequences covering 23 provinces and eight species, spanning the second and third epidemics. Specifically, we investigated whether the three epidemics are associated with a single reemerging lineage or a different lineage was responsible for each epidemic. Consistent with previous results, phylogenetic analysis identified six lineages, China I to VI. Analysis of the geographical composition of these lineages revealed they are consistent with human case data and reflect the gradual emergence of China I in the third epidemic. Initially, China I was restricted to south China and China II was dominant. However, as the epidemic began to spread into new areas, China I began to emerge, whereas China II remained confined to south China. By the latter part of the surveillance period, almost all isolates were China I and contributions from the remaining lineages were minimal. The prevalence of China II in the early stages of the third epidemic and its established presence in wildlife suggests that it too replaced a previously dominant lineage during the second epidemic. This lineage replacement may be a consequence of control programs that were dominated by dog culling efforts as the primary control method in the first two epidemics. This had the effect of reducing dominant strains to levels comparable with other localized background stains. Our results indicate the importance of effective control strategies for long term control of the disease.
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- 2013
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13. Mosquitoes of Western Yunnan Province, China: seasonal abundance, diversity, and arbovirus associations.
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Hai-Lin Zhang, Yu-Zhen Zhang, Wei-Hong Yang, Yun Feng, Roger S Nasci, Jie Yang, Yong-Hua Liu, Chao-Liang Dong, Shi Li, Bao-Sen Zhang, Zheng-Liu Yin, Pi-Yu Wang, Shi-Hong Fu, Ming-Hua Li, Fen Liu, Juan Zhang, Jie Sun, Can-Wei Li, Xiao-Yan Gao, Hong Liu, Huan-Yu Wang, Lyle R Petersen, and Guo-Dong Liang
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Medicine ,Science - Abstract
OBJECTIVE:The western borderland between Yunnan Province, China, and Myanmar is characterized by a climate that facilitates year-round production of mosquitoes. Numerous mosquito-transmitted viruses, including Japanese encephalitis virus circulate in this area. This project was to describe seasonal patterns in mosquito species abundance and arbovirus activity in the mosquito populations. METHODS:Mosquitoes were collected in Mangshi and Ruili cities of Dehong Prefecture near the border of China and Burma in Yunnan Province, the Peoples Republic of China in 2010. We monitored mosquito species abundance for a 12-month period using ultraviolet light, carbon dioxide baited CDC light and gravid traps; and tested the captured mosquitoes for the presence of virus to evaluate mosquito-virus associations in rural/agricultural settings in the area. RESULTS:A total of 43 species of mosquitoes from seven genera were collected, including 15 Culex species, 15 Anopheles spp., four Aedes spp., three Armigeres spp., one Mimomyia spp., two Uranotaenia spp. and three Mansonia spp.. Species richness and diversity varied between Mangshi and Ruili. Culex tritaeniorhynchus, Culex quinquefasciatus, Anopheles sinensis and Anopheles peditaeniatus were the most abundant species in both sampling sites. Ultraviolet light traps collected more specimens than CDC light traps baited with dry ice, though both collected the same variety of mosquito species. The CDC gravid trap was the most effective trap for capture of Culex quinquefasciatus, a species underrepresented in light trap collections. A total of 26 virus strains were isolated, which included 13 strains of Japanese encephalitis virus, four strains of Getah virus, one strain of Oya virus, one strain from the orbivirus genus, and seven strains of Culex pipien pallens densovirus. CONCLUSIONS:The present study illustrates the value of monitoring mosquito populations and mosquito-transmitted viruses year-round in areas where the climate supports year-round adult mosquito activity.
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- 2013
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14. Japanese Encephalitis in China in the Period of 1950-2018: From Discovery to Control
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Xiao Jing, Chen, Huan Yu, Wang, Xiao Long, Li, Xiao Yan, Gao, Ming Hua, Li, Shi Hong, Fu, Ying, He, Fan, Li, Qi Kai, Yin, Song Tao, Xu, Dan, Wu, Yi Xing, Li, Zun Dong, Yin, Guang, Yang, and Guo Dong, Liang
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China ,Vaccination ,Humans ,Viral Vaccines ,Encephalitis, Japanese - Published
- 2020
15. Absence of association between N-acetyltransferase 2 acetylator status and colorectal cancer susceptibility: based on evidence from 40 studies.
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Lou qian Zhang, Jian nong Zhou, Jun Wang, Guo dong Liang, Jing ying Li, Yi dan Zhu, and Yun tao Su
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Medicine ,Science - Abstract
BACKGROUND AND OBJECTIVES: N-Acetyltransferase (NAT) 2 is an important enzyme involved in the metabolism of different xenobiotics, including potential carcinogens, whose phenotypes were reported to be related to individual susceptibility to colorectal cancer (CRC). However, the results remain conflicting. To assess the relationship between NAT2 phenotypes and CRC risk, we performed this meta-analysis. METHODS: A comprehensive literature search was conducted to identify all case-control or cohort studies of NAT2 acetylator status on the susceptibility of CRC by searching of PubMed and EMBASE, up to May 20, 2011. Crude odds ratios (ORs) with 95% confidence intervals (CIs) were used to assess the association. RESULTS: A total of over 40,000 subjects from 40 published literatures were identified by searching the databases. No significantly elevated CRC risk in individuals with NAT2 slow acetylators compared with fast acetylators was found when all studies pooled (OR = 0.95, 95% CI: 0.87-1.04, I(2) = 52.6%). While three studies contributed to the source of heterogeneity were removed, there was still null result observed (OR = 0.96, 95% CI: 0.90-1.03, P = 0.17 for heterogeneity, I(2) = 17.8%). In addition, we failed to detect any associations in the stratified analyses by race, sex, source of controls, smoking status, genotyping methods or tumor localization. No publication bias was observed in this study. CONCLUSIONS: This meta-analysis suggests that the NAT2 phenotypes may not be associated with colorectal cancer development.
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- 2012
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16. Lamellar orientation in thin films of symmetric semicrystalline polystyrene-b-poly(ethylene-co-butene) block copolymers: effects of molar mass, temperature of solvent evaporation, and annealing
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Guo-Dong Liang, Jun-Ting Xu, and Zhi-Qiang Fan
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Atomic force microscopy -- Usage ,Chemicals, plastics and rubber industries - Abstract
Atomic force microscopy, grazing incidence X-ray diffraction and X-ray photoelectron spectroscopy were used for examining the orientation of the lamellar microdomains in thin films of three symmetric polystyrene-b-poly(ethylene-co-butylene) block copolymers on mica. Findings reveal the dependence of the lamellar orientation in the [S.sub.x][E.sub.y] block copolymers on the molar mass of block copolymers, the temperature of solvent evaporation and annealing.
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- 2007
17. Genotype v Japanese encephalitis virus is emerging.
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Ming-Hua Li, Shi-Hong Fu, Wei-Xin Chen, Huan-Yu Wang, Yu-Hong Guo, Qi-Yong Liu, Yi-Xing Li, Hui-Ming Luo, Wa Da, Dun Zhu Duo Ji, Xiu-Min Ye, and Guo-Dong Liang
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Arctic medicine. Tropical medicine ,RC955-962 ,Public aspects of medicine ,RA1-1270 - Abstract
Japanese encephalitis (JE) is a global public health issue that has spread widely to more than 20 countries in Asia and has extended its geographic range to the south Pacific region including Australia. JE has become the most important cause of viral encephalitis in the world. Japanese encephalitis viruses (JEV) are divided into five genotypes, based on the nucleotide sequence of the envelope (E) gene. The Muar strain, isolated from patient in Malaya in 1952, is the sole example of genotype V JEV. Here, the XZ0934 strain of JEV was isolated from Culex tritaeniorhynchus, collected in China. The complete nucleotide and amino acid sequence of XZ0934 strain have been determined. The nucleotide divergence ranged from 20.3% to 21.4% and amino acid divergence ranged from 8.4% to 10.0% when compared with the 62 known JEV isolates that belong to genotype I-IV. It reveals low similarity between XZ0934 and genotype I-IV JEVs. Phylogenetic analysis using both complete genome and structural gene nucleotide sequences demonstrates that XZ0934 belongs to genotype V. This, in turn, suggests that genotype V JEV is emerging in JEV endemic areas. Thus, increased surveillance and diagnosis of viral encephalitis caused by genotype V JEV is an issue of great concern to nations in which JEV is endemic.
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- 2011
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18. Serological Survey of Zika Virus in Humans and Animals in Dejiang Prefecture, Guizhou Province, China
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Fan, Li, Jing Zhu, Zhou, Lei, Zhou, Shi Hong, Fu, Zhen Zao, Tian, Qi, Wang, Nan, Shao, Dan, Li, Ying, He, Wen Wen, Lei, Guang Peng, Tang, Guo Dong, Liang, Ding Ming, Wang, Yan Ping, Zhang, and Huan Yu, Wang
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Adult ,Male ,China ,Adolescent ,Infant, Newborn ,Infant ,Zika Virus ,Middle Aged ,Risk Assessment ,Young Adult ,Child, Preschool ,Surveys and Questionnaires ,Animals ,Humans ,Female ,Serologic Tests ,Child ,Aged - Abstract
The current outbreak of Zika virus (ZIKV) poses a severe threat to human health. Two ZIKV strains were isolated from mosquitoes collected from the Dejiang prefecture in China in 2016, which was the first isolation of ZIKV in nature in China.In this study, serum samples were collected from 366 healthy individuals and 104 animals from Dejiang prefecture in 2017, and the plaque reduction neutralization test (PRNT) was used to evaluate the seroprevalence of ZIKV.None of the 366 residents from whom the samples were collected were seropositive for ZIKV. None of the 11 pigs from whom the samples were collected were seropositive for ZIKV, while 1 of 63 (1.59%) chickens and 2 of 30 (6.67%) sheep were seropositive for ZIKV.The extremely low seropositivity rate of ZIKV antibodies in animals in the Dejiang prefecture, Guizhou province in this study indicates that ZIKV can infect animals; however, there is a low risk of ZIKV circulating in the local population.
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- 2019
19. A Reverse-transcription Recombinase-aided Amplification Assay for the Rapid Detection of the Far-Eastern Subtype of Tick-borne Encephalitis Virus
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Qian Ying, Wang, Fan, Li, Xin Xin, Shen, Shi Hong, Fu, Ying, He, Wen Wen, Lei, Guo Dong, Liang, Huan Yun, Wang, and Xue Jun, Ma
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RNA, Viral ,Nucleic Acid Amplification Techniques ,Encephalitis Viruses, Tick-Borne - Abstract
Tick-borne encephalitis virus (TBEV) is an emerging pathogen in Europe and North Asia that causes tick-borne encephalitis (TBE). A simple, rapid method for detecting TBEV RNA is needed to control this disease.A reverse-transcription recombinase-aided amplification (RT-RAA) assay was developed. This assay can be completed in one closed tube at 39 °C within 30 minutes. The sensitivity and specificity of RT-RAA were validated using non-infectious synthetic RNA representing a fragment of the NS5 region of the wild-type (WT) TBEV genome and the Senzhang strain. Additionally, 10 batches of tick samples were used to evaluate the performance of the RT-RAA assay.The analytical limit of detection of the assay was 20 copies per reaction of the TBEV synthetic transcript and 3 plaque-forming units (pfu) per reaction of TBEV titers. With the specific assay, no signal due to other arboviruses was observed. Of the 10 batches of tick samples obtained from the Changbai Mountains of China, three were TBEV-positive, which was consistent with the results of the quantitative real-time PCR assay.A rapid, highly sensitive, specific, and easy-to-use method was developed for the detection of the TBEV Far-Eastern subtype.
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- 2019
20. Japanese encephalitis outbreak, Yuncheng, China, 2006
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Li-Hua, Wang, Shi-Hong, Fu, Huan-Yu, Wang, Xiao-Feng, Liang, Jing-Xia, Cheng, Hong-Mei, Jing, Gen-Lao, Cai, Xing-Wang, Li, Wen-Yuan, Ze, Xin-Jun, Lv, Hua-Qing, Wang, Ding-Lin, Zhang, Yun, Feng, Zun-Dong, Yin, Xiao-Hong, Sun, Tie-Jun, Shui, Ming-Hua, Li, Yi-Xing, Li, and Guo-Dong, Liang
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Japanese encephalitis -- Care and treatment ,Japanese encephalitis -- Diagnosis ,Japanese encephalitis -- Development and progression ,China -- Development and progression ,China -- Diagnosis ,China -- Care and treatment - Abstract
To the Editor: Japanese encephalitis (JE) epidemics have occurred only in Asia. More than 50,000 cases of JE with [approximately equal to] 10,000 deaths have been reported since 1998 (1,2). [...]
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- 2007
21. TaqMan Real-time RT-PCR Assay for Detecting and Differentiating Japanese Encephalitis Virus
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Nan, Shao, Fan, Li, Kai, Nie, Shi Hong, Fu, Wei Jia, Zhang, Ying, He, Wen Wen, Lei, Qian Ying, Wang, Guo Dong, Liang, Yu Xi, Cao, and Huan Yu, Wang
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Encephalitis Virus, Japanese ,Culicidae ,Animals ,Reproducibility of Results ,Polymerase Chain Reaction ,Sensitivity and Specificity - Abstract
To detect Japanese encephalitis virus (JEV) rapidly and distinguish its genotypes, a TaqMan-based reverse transcriptase quantitative polymerase chain reaction (RT-PCR) detection system was developed.By aligning the full-length sequences of JEV (G1-G5), six sets of highly specific TaqMan real-time RT-PCR primers and probes were designed based on the highly conserved NS1, NS2, and M genes of JEV, which included one set for non-specific JEV detection and five sets for the detection of specific JEV genotypes. Twenty batches of mosquito samples were used to evaluate our quantitative PCR assay.With the specific assay, no other flavivirus were detected. The lower limits of detection of the system were 1 pfu/mL for JEV titers and 100 RNA copies/µL. The coefficients of variation of this real-time RT-PCR were all2.8%. The amplification efficiency of this method was between 90% and 103%.A TaqMan real-time RT-PCR detection system was successfully established to detect and differentiate all five JEV genotypes.
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- 2018
22. Research on basis of reverse genetics system of a Sindbis-like virus XJ-160
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Wu-yang Zhu and Guo-dong Liang
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Infectious and parasitic diseases ,RC109-216 - Abstract
Abstract As a Sindbis-like virus (SINLV), XJ-160 virus was isolated from a pooled sample of Anopheles mosquitoes collected in Xinjiang, China, in 1990. Recombinant plasmid pBR-XJ160 is an infectious full-length cDNA clone of XJ-160 virus, from which rescued virus BR-XJ160 can be obtained by transcription in vitro and transfection. The BR-XJ160 virus raised in BHK-21 cells was indistinguishable from the XJ-160 virus in its biological properties, including its plaque morphology, growth kinetics and suckling mouse neurovirulence. On basis of pBR-XJ160, the effects of substitutions within nonstructural protein 1 (nsP1) or nsP2 on the infectivity and pathogenesis of Sindbis virus (SINV) have been investigated. We have also confirmed the essential role of E2 glycoprotein, especially the domain of 145-150 (amino acid) aa, in SINV infection through the interaction with cellular heparan sulfate (HS). In addition, we have developed XJ-160 virus-based vector system, including replicon vector, defective helper (DH) plasmids and the packaging cell lines (PCLs). Here we provide an update of main development in the field concerned with XJ-160 virus.
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- 2011
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23. Research on Monitoring System of Gas Flow Meters Based on ZigBee Wireless Network
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Guo Dong Liang, Xing Xing Mao, Bing Yan, Li Qiang Wang, Jing Zhu, and Guang Zhao
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Engineering ,Wi-Fi array ,business.industry ,Wireless network ,Node (networking) ,ComputerSystemsOrganization_COMPUTER-COMMUNICATIONNETWORKS ,Serial port ,Wireless WAN ,General Medicine ,Wireless ,business ,Wireless sensor network ,Computer network ,NeuRFon - Abstract
ZigBee is a low-rate, low-power-consumption wireless communication technology, which can be widely used in the Wireless Sensor Network (WSN). ZigBee wireless network is applied to the monitoring system of gas flow meters in this paper. The design of hardware and software of the system based on Zigbee chip is given. The gas flow data is uploaded to the terminal node via RS485 serial port, and the terminal node transmits the data to the coordinator by the ZigBee wireless network, and then the coordinator sends the data to the PC by using RS232 serial port. In this way, the monitoring center can manage and analyze the data, so the real-time monitoring can be realized. It has been proved that the system has strong anti-interference ability, safe and reliable and stable operation.
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- 2015
24. The Relationship between Japanese Encephalitis and Environmental Factors in China Explored Using National Surveillance Data
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Xiao Xia, Huang, Lei, Yan, Xiao Yan, Gao, Yu Huan, Ren, Shi Hong, Fu, Yu Xi, Cao, Ying, He, Wen Wen, Lei, Guo Dong, Liang, Shi Wen, Wang, and Huan Yu, Wang
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China ,Risk Factors ,Incidence ,Epidemiological Monitoring ,Humans ,Environment ,Encephalitis, Japanese - Abstract
Japanese encephalitis (JE) is a serious public health issue. This study was undertaken to better understand the relationship between JE distribution and environmental factors in China. JE data from 2005 to 2010 were retrieved from National Notifiable Disease Report System. ArcGIS, remote sensing techniques, and R software was used to exhibit and explore the relationship between JE distribution and environmental factors. Our results indicated that JE cases were mostly concentrated in warm-temperate, semitropical and tropical zones with annual precipitation400 mm; Broad-leaved evergreen forest, shrubs, paddy field, irrigated land, dryland, evergreen coniferous forest, and shrubland were risk factors for JE occurrence, and the former five were risk factors for counties with high JE incidence. These findings will inform the effective allocation of limited health resources such as intensive vaccination, surveillance and training in areas with high environmental risk factors.
- Published
- 2017
25. An anatomic study of trifurcate iliotibial bands for correcting valgus knee deformity
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Yuntao Wu, Guo-dong Liang, Qingsan Zhu, Yuhang Zhu, Qin-li Xu, and You-qiong Li
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Adult ,Male ,Knee Joint ,Total knee arthroplasty ,Knee deformity ,03 medical and health sciences ,0302 clinical medicine ,Cadaver ,Medicine ,Humans ,Orthopedics and Sports Medicine ,Fascia ,Aged ,030222 orthopedics ,biology ,Subject Age ,business.industry ,Body Weight ,Age Factors ,030229 sport sciences ,Anatomy ,Middle Aged ,biology.organism_classification ,Body Height ,Fasciotomy ,Genu Valgum ,Valgus ,Female ,Negative correlation ,business - Abstract
Background The iliotibial band (ITB) trifurcates into the anterior, central and posterior branches at the knee level, and sometimes the branches must be selectively released to correct the valgus knee deformity during total knee arthroplasty. However, the anatomical morphology of the trifurcate ITBs has not been investigated. Methods Fifty-two knees from 26 embalmed cadavers were dissected to observe and record the relationship of the three branches given off from the ITB trifurcation. Fourteen parameters with regard to the length, width, thickness, and trifurcate angle of each branch were measured. These parameters were compared between sex and sides (left or right). Meanwhile, the correlations between parameters and subject age, weight and height were assessed. Results The longest, widest and thickest branches of the ITB were the posterior band (59.82 ± 5.14 mm), anterior band (39.56 ± 4.17 mm) and central band (2.61 ± 0.36 mm), respectively. The length and thickness of ITB were significantly larger in males than in females (P 0.05). The ITB thickness showed a negative correlation with subject age, while the length and width of the ITB were positively correlated with subject height and weight, respectively. Conclusions This study provided an anatomical reference of trifurcate ITBs to help the release of ITB in valgus knees. The anatomical variations regarding the subject's sex, age, height and weight should be considered in the selective release of ITB.
- Published
- 2017
26. The Injection Mold Design of Clamshell Phone Upper Cover
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Guo Dong Liang and Jun Hui Liu
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Clamshell ,Engineering ,business.product_category ,business.industry ,General Engineering ,Injector ,Structural engineering ,medicine.disease_cause ,Rod ,law.invention ,law ,Mold ,Water cooling ,medicine ,Die (manufacturing) ,Cover (algebra) ,business ,Buckle - Abstract
The design of injection die on the upper cover of clamshell phone is introduced. The dual-plate die has side gate for opposite glue injection, the parts are ejected by ejector pin, the side reversed buckle contains three blocks, the reversed buckle at flip shaft consists of two inner sliders, and all the eight inner reversed buckles are made up of inclined pushing rods, and the cooling system embraces "U" shaped cooling waterway. Its application in practice has proved the reasonable die structure, smooth remolding, and qualified plastic part design.
- Published
- 2014
27. Injection Mould Design of Mobile Phone Back Shell
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Guo Dong Liang and Feng Liang
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Engineering ,Engineering drawing ,business.industry ,Shell (computing) ,General Engineering ,Mechanical engineering ,Injector ,Molding (process) ,Edge (geometry) ,medicine.disease_cause ,Stripping (fiber) ,law.invention ,Coolant ,law ,Mold ,medicine ,Water cooling ,business - Abstract
This article introduces the injection mould design of mobile phone back shell. The mould adopts 3-plate mold structure, pin-point gate for feeding in the glue, ejector pin and pieces for stripping. The under-cut at the side edge adopts 3-side sliding block molding, and the cooling system adopts “U”-shape coolant gallery. It is proved by production practice that the mould structure is reasonable, stripping smoothly and the plastic parts quality meets the design requirements.
- Published
- 2014
28. Specific Nucleotide Changes in the Subgenomic Promoter Region Influence Infectivity of the Sindbis Virus
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Guo-dong Liang, Wu-yang Zhu, and Ting Zhao
- Subjects
Sindbis virus ,Transcription, Genetic ,Short Communication ,Viral Plaque Assay ,Virus ,Cell Line ,law.invention ,Transcription (biology) ,law ,Cricetinae ,Virology ,Animals ,Point Mutation ,RNA, Messenger ,Promoter Regions, Genetic ,Sequence Deletion ,Subgenomic mRNA ,Infectivity ,biology ,Chemistry ,Point mutation ,Promoter ,Viral Load ,biology.organism_classification ,Molecular biology ,Infectious Diseases ,Recombinant DNA ,RNA, Viral ,Subgenomic promoter - Abstract
Transcription of the subgenomic mRNA of Sindbis virus (SINV) is initiated at a subgenomic promoter (SP). Alignment of SINV sequences identified a 68-nucleotide conserved domain spanning -19 to +49 relative to the subgenomic mRNA start site. Nucleotide T or C is present at -18 or +49 in all known SINVs while a Sindbis-like virus XJ-160 has an A or T at a corresponding position. Our results indicate that deletion or substitution of the T at +49 decreased the activity of SP, while substituting T for A at -18 did not decrease the activity of SP or genetic stability of recombinant SINV.
- Published
- 2013
29. Identification of a Newly Isolated Getah Virus in the China-Laos Border, China
- Author
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Yuan Yuan, Li, Shi Hong, Fu, Xiao Fang, Guo, Wen Wen, Lei, Xiao Long, Li, Jing Dong, Song, Lei, Cao, Xiao Yan, Gao, Zhi, Lyu, Ying, He, Huan Yu, Wang, Xiao Jie, Ren, Hong Ning, Zhou, Gui Qin, Wang, and Guo Dong, Liang
- Subjects
China ,Culicidae ,Laos ,Cricetinae ,Animals ,Alphavirus ,Phylogeny ,Cell Line - Abstract
In this study, we isolated a virus strain (YN12031) from specimens of Armigeres subalbatus collected in the China-Laos border. BHK-21 cells infected with YN12031 exhibited an evident cytopathic effect (CPE) 32 h post-infection. The virus particles were spherical, 70 nm in diameter, and enveloped; they also featured surface fibers. Molecular genetic analysis revealed that YN12031 was closely related to alpha viruses such as Chikungunya virus and Sindbis virus, and located in the same clade as MM2021, the prototype of Getahvirus (GETV) isolated in Malaysia in 1955. Phylogenetic analysis of the E2 and capsid genes further revealed that YN12031 was located in the same clade as the Russian isolate LEIV/16275/Mag. Analysis of the homology of nucleotides and amino acids in the coding area and E2 gene demonstrated that the YN12031 isolated from the China-Laos border (tropical region) was related closest to the LEIV/16275/Mag isolate obtained in Russia (North frigid zone area) among other isolates studied. These results suggest that GETV can adapt to different geographical environments to propagate and evolve. Thus, strengthening the detection and monitoring of GETV and its related diseases is very crucial.
- Published
- 2016
30. A Centralized Report on Pediatric Japanese Encephalitis Cases from Beijing Children's Hospital, 2013
- Author
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Jiu Wei, Li, Xiao Yan, Gao, Yun, Wu, Shi Hong, Fu, Xiao Juan, Tan, Yu Xi, Cao, Wei Hua, Zhang, Zun Dong, Yin, Ying, He, Yi Xing, Li, Guo Dong, Liang, Wen Bo, Xu, Fang, Fang, and Huan Yu, Wang
- Subjects
Encephalitis Virus, Japanese ,Male ,Japanese Encephalitis Vaccines ,Beijing ,Child, Preschool ,Humans ,Female ,Child ,Encephalitis, Japanese ,Prognosis - Abstract
Fifteen pediatric cases of suspected Japanese encephalitis (JE) were reported in Beijing Children's Hospital during the late summer of 2013. The clinical manifestations in most cases included high fever, seizures, and abnormal magnetic resonance imaging findings. Twelve of 15 cases were laboratory-confirmed as JE cases by pathogen identification. Epidemiological investigations showed that five of the 12 laboratory-confirmed patients had an incomplete JE vaccination history. Follow-up investigations after discharge indicated that seven laboratory-confirmed JE patients without JE vaccinations had relatively poor prognoses, with an average Modified Rankin Scale (MRS) score of 2.6 when compared with the other five laboratory-confirmed, JE-vaccinated patients with an average MRS score of 0.5. The observation of pediatric JE cases among those with a history of JE vaccination warrants further attention.
- Published
- 2016
31. Study on the protective efficacy of SA14-14-2 attenuated Japanese encephalitis against different JE virus isolates circulating in China
- Author
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Lili Jia, Huan-yu Wang, Guo-dong Liang, Mao-Guang Li, Yongxin Yu, Xinyu Liu, and Guan-Mu Dong
- Subjects
China ,Genotype ,viruses ,Biology ,Antibodies, Viral ,Vaccines, Attenuated ,Virus ,Microbiology ,Mice ,Neutralization Tests ,medicine ,Animals ,Humans ,Encephalitis, Japanese ,Encephalitis Virus, Japanese ,Attenuated vaccine ,General Veterinary ,General Immunology and Microbiology ,Japanese Encephalitis Vaccines ,Public Health, Environmental and Occupational Health ,Japanese encephalitis ,medicine.disease ,Vaccine efficacy ,biology.organism_classification ,Virology ,Vaccination ,Flavivirus ,Culicidae ,Infectious Diseases ,Molecular Medicine ,Encephalitis - Abstract
Prior to 1976 only Japanese encephalitis virus (JEV) genotype III could be detected in China. Recently, numerous genotype I JEV strains have been isolated from JE patients, mosquitoes and pigs while genotype III strains remain present. Two kinds of JEV vaccines are currently used in China for the prevention disease: the JE live attenuated vaccine (LAV) SA14-14-2 virus and the inactivated P3 strain (IPV) vaccine. The SA14-14-2 and P3 viral strains were isolated in the year of 1953 and 1949 respectively and both belonged to the JEV genotype III. In order to evaluate the protective efficacy of both vaccines against the JEV genotype I isolates we conducted vaccination-challenge protection assays in mice. These data demonstrated that both LAV (≥ 234 pfu virus) and IPV (1:5 dilution) vaccines effectively conferred protection against all 16 isolates tested following intraperitoneal (i.p.) challenge. However, when vaccinated mice were challenged via intracerebral (i.c.) injection, ≥ 60% LAV vaccinated animals were protected against challenge with most JEV isolates but only ≤ 40% protection was observed following vaccination with IPV. These results indicated that JE vaccines used in China still protected effectively against both JEV genotypes now prevalent in China and that the LAV formulation conferred higher levels of protection compared to the protection conferred by IPV.
- Published
- 2011
32. Time Evolution, Dynamical Quantum Fluctuation and High-Order Squeezing Feature in Polariton System — I
- Author
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Chao-Fan Yu, Xiao-Min Yu, and Guo-Dong Liang
- Subjects
Condensed Matter::Quantum Gases ,Physics ,Photon ,Physics and Astronomy (miscellaneous) ,Phonon ,Time evolution ,Quantum Physics ,symbols.namesake ,Nonlinear system ,Quantum electrodynamics ,Quantum mechanics ,Polariton ,symbols ,High order ,Hamiltonian (quantum mechanics) ,Quantum fluctuation - Abstract
We have set up a new reduced model Hamiltonian for the polariton system, in which the nonlinear interaction contains the rotating term k1(a+b + ab+) and the attractive two-mode squeezed coupling −k2(a+b+ +ab). The dynamical evolution of this system has been solved and the nonclassical features relevant to the second-order and high-order squeezing have been obtained in an analytical form. For the first time, in contrast to the existing result, we have confirmed for the phonon field that the attractive two-mode squeezed interaction will not only result in the second-order and high-order squeezing in X-component with the time evolution, but also in time average. Furthermore, the phenomena of collapse and revival of inversion will occur as well in the time evolution of the average number of photon and phonon, as also in the second-order and high-order squeezing of photon field, particularly, in the high-order squeezing of phonon field.
- Published
- 2010
33. Tahyna Virus and Human Infection, China
- Author
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Qiang Dong, Guo Dong Liang, Amy J. Lambert, Huan Yu Wang, Yougang Zhai, Yu Ping Feng, Su Xiang Tong, Feng Tian Wang, Qing Tang, Zhi Lu, Shi Hong Fu, Da Xin Ni, Xin Hua Yao, Song Zhang, Roger S. Nasci, Yun Feng, Xin-jun Lü, Zhao Xia Li, and Xiao Yan Gao
- Subjects
Microbiology (medical) ,Adult ,Male ,China ,Epidemiology ,Culex ,viruses ,Bunyaviridae ,California group viruses ,lcsh:Medicine ,Encephalitis Virus, California ,Tahyna virus ,Antibodies, Viral ,Virus ,Immunoglobulin G ,Cell Line ,lcsh:Infectious and parasitic diseases ,Young Adult ,Encephalitis, California ,Cricetinae ,Veterinary virology ,Chlorocebus aethiops ,parasitic diseases ,Animals ,Humans ,lcsh:RC109-216 ,Child ,Vero Cells ,Phylogeny ,Aged ,biology ,lcsh:R ,dispatch ,Sequence Analysis, DNA ,Middle Aged ,biology.organism_classification ,Virology ,Infectious Diseases ,Immunoglobulin M ,biology.protein ,Vero cell ,Antibody - Abstract
In 2006, Tahyna virus was isolated from Culex spp. mosquitoes collected in Xinjiang, People’s Republic of China. In 2007, to determine whether this virus was infecting humans, we tested serum from febrile patients. We found immunoglobulin (Ig) M and IgG against the virus, which suggests human infection in this region.
- Published
- 2009
34. High density fermentation and activity of a recombinant lumbrokinase (PI239) from Pichia pastoris
- Author
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Huan-Yu Wang, Qing Tang, Shi-Hong Fu, Guo-Dong Liang, Kun-Ping Guan, Li-hong Xu, and Tao Ge
- Subjects
Thrombin time ,Pichia ,Pichia pastoris ,law.invention ,Fungal Proteins ,In vivo ,law ,Endopeptidases ,medicine ,Lumbrokinase ,DNA Primers ,biology ,medicine.diagnostic_test ,Chemistry ,Fibrinolysis ,biology.organism_classification ,Recombinant Proteins ,In vitro ,Yeast ,Molecular Weight ,Kinetics ,Biochemistry ,Fermentation ,Prothrombin Time ,Recombinant DNA ,Genetic Engineering ,Plasmids ,Biotechnology - Abstract
A system for the expression of recombinant lumbrokinase (rPI239) was developed in the yeast Pichia pastoris. A total supernatant protein content of 0.174 g/L of high density fermentation broth was obtained. The rPI239 exhibited in vitro fibrinolytic activity. The in vivo activity of rPI239 was measured by prothrombin time, kaolin part thrombin time, thrombin time, and fibrinolytic activity. This work presents the high-density fermentation of rPI239 from P. pastoris and shows that the recombinant protein has similar fibrinolytic activity both in vivo and in vitro.
- Published
- 2007
35. Molecular epidemiological analysis of Japanese encephalitis virus in China
- Author
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Jia Ke Zhang, Ichiro Kurane, Xiao Feng Liang, Qing Tang, Zong Yu Hao, Shi Hong Fu, Huan Yu Wang, Guo Dong Liang, Shigeru Tajima, Hai Lin Zhang, Tomohiko Takasaki, Wei Zhong Yang, Xiao-hong Sun, Zhao Xiao Wang, and Akira Kotaki
- Subjects
China ,Genes, Viral ,viruses ,Molecular Sequence Data ,Sequence Homology ,Ceratopogonidae ,Virus ,Viral Proteins ,Flaviviridae ,Viral Envelope Proteins ,Virology ,Genotype ,medicine ,Animals ,Humans ,Encephalitis, Japanese ,Phylogeny ,Cerebrospinal Fluid ,Encephalitis Virus, Japanese ,Molecular Epidemiology ,Membrane Glycoproteins ,Phylogenetic tree ,biology ,Molecular epidemiology ,Sequence Analysis, DNA ,Japanese encephalitis ,medicine.disease ,biology.organism_classification ,Flavivirus ,Blood ,Culicidae ,RNA, Viral ,Encephalitis - Abstract
Sixty-two new Japanese encephalitis virus (JEV) isolates were obtained from mosquitoes, biting midges, human cerebrospinal fluid and human blood samples in China during 2002–2005. The E and prM genes were sequenced and phylogenetic analyses were performed with 38 JEV other isolates from China and 36 JEV strains from other countries. Phylogenetic trees based on the E and prM gene sequences were similar. The results indicate that: (i) recent JEV isolates from China are divided into two genotypes, genotype 1 and genotype 3; (ii) recent JEV isolates from China are grouped into the same clusters within genotypes 1 and 3; and (iii) genotype 1 JEV strains have been isolated in China since 1979, whilst genotype 3 JEV strains were isolated before the 1970s. The results suggest that genotype 1 JEV was introduced to China around 1979 and that JEV strains belonging to genotypes 1 and 3 circulate in China.
- Published
- 2007
36. The effects of different levels of superoxide dismutase in Modena on boar semen quality during liquid preservation at 17°C
- Author
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Xiao-Gang, Zhang, Hao, Li, Le, Wang, Yang-Yi, Hao, Guo-Dong, Liang, Yun-Hui, Ma, Gong-She, Yang, and Jian-Hong, Hu
- Subjects
Male ,Dose-Response Relationship, Drug ,Cell Survival ,Superoxide Dismutase ,Swine ,Acrosome Reaction ,Temperature ,Hydrogen Peroxide ,Spermatozoa ,Antioxidants ,Oxidative Stress ,Malondialdehyde ,Sperm Motility ,Animals ,Semen Preservation - Abstract
This study was conducted to investigate the influence of superoxide dismutase (SOD) on the quality of boar semen during liquid preservation at 17°C. Semen samples from 10 Duroc boars were collected and pooled, divided into five equal parts and diluted with Modena containing different concentrations (0, 100, 200, 300 and 400 U/mL) of SOD. During the process of liquid preservation at 17°C, sperm motility, acrosome integrity, membrane integrity, total antioxidant capacity (T-AOC) activity, malondialdehyde (MDA) content and hydrogen peroxide (H
- Published
- 2015
37. Isolation and full-length genome analysis of mosquito-borne Manzanilla virus from Yunnan Province, China
- Author
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W.H. Yang, Chang‑Chun Tu, Shi Hong Fu, Hai‑Lin Zhang, Yun Feng, Biao He, Guo Dong Liang, and Yu Zhen Zhang
- Subjects
China ,Orthobunyavirus ,Culex ,Short Report ,Genome, Viral ,Biology ,Oya virus ,Genome ,General Biochemistry, Genetics and Molecular Biology ,Homology (biology) ,Viral Proteins ,Phylogenetics ,parasitic diseases ,Animals ,Amino Acid Sequence ,Manzanilla virus ,Gene ,Peptide sequence ,Phylogeny ,Genetics ,Medicine(all) ,Base Sequence ,Sequence Homology, Amino Acid ,Biochemistry, Genetics and Molecular Biology(all) ,General Medicine ,biology.organism_classification ,Culex tritaeniorhynchus - Abstract
Background There have been four strains on Manzanilla virus (MANV) identified to date. Here, we identify a novel MANV strain (DHL10M107) isolated from Culex tritaeniorhynchus Giles mosquitoes from Ruili city, Dehong prefecture, Yunnan Province, in the People’s Republic of China. Results The DHL10M107 L, M and S genes were sequenced at the nucleotide and deduced amino acid levels. The L, M and S gene sequences of DHL10M107 clustered with the MANV strains VN04-2108, TRVL3587, SA An 4165, and AV 782. DHL10M107 was most closely related to VN04-2108. Nucleotide homology ranged between 96 and 99% between DHL10M107 and VN04-2108. In terms of amino acid homology, all of the amino acid differences were in the L (96.3% homologous) and M (97.7% homologous) fragments. Conclusions DHL10M107 is likely a MANV isolated from mosquitos in the Yunnan Province. This is the first reported isolation of MANV in mainland China. Electronic supplementary material The online version of this article (doi:10.1186/s13104-015-1198-5) contains supplementary material, which is available to authorized users.
- Published
- 2015
38. The expression of membrane protein augments the specific responses induced by SARS-CoV nucleocapsid DNA immunization
- Author
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Yin Chuan Li, Jin Ling Wang, Guo Dong Liang, Shu Qun Shi, Chuan Qin, Li Xu, Quan Hong Sun, Jing Pian Peng, and Ying Yang
- Subjects
CD4-Positive T-Lymphocytes ,DNA immunization ,Myeloma protein ,viruses ,Immunology ,Gene Expression ,Pathogenesis ,Antigen-Antibody Complex ,CD8-Positive T-Lymphocytes ,Biology ,Antibodies, Viral ,medicine.disease_cause ,Article ,Virus ,law.invention ,DNA vaccination ,Mice ,Immune system ,Antigen ,law ,Vaccines, DNA ,medicine ,Animals ,Nucleocapsid ,Lung ,Molecular Biology ,Cell Proliferation ,Coronavirus ,Mice, Inbred BALB C ,Arvicolinae ,Membrane ,Membrane Proteins ,SARS-CoV ,Nucleocapsid Proteins ,Flow Cytometry ,Molecular biology ,Severe acute respiratory syndrome-related coronavirus ,DNA, Viral ,Recombinant DNA ,biology.protein ,Cytokines ,Antibody - Abstract
Nucleocapsid protein plays a critical role in SARS-CoV pathogenesis, and high-level anti-nucleocapsid antibodies are detected in the patients infected by severe acute respiratory syndrome-associated coronavirus (SARS-CoV). Several studies have shown that there exists an interaction between nucleocapsid (N) and membrane (M) protein. In this paper, we investigate whether the expression of membrane protein can affect the immune responses induced by nucleocapsid DNA immunization. Two recombinant plasmids containing M and N coding sequence were constructed. Moreover, in order to get the antigen for ELISA and in vitro stimulation assay, N protein were expressed and purified from E. coli bacteria. Injection of 20mug of the mixture of pVAX1-M and pVAX1-N into the Balb/c mice could elicit the humoral and cellular responses. The ELISA analysis using the N antigen or inactivated SARS-CoV particles as capture antigen showed that co-injection of SARS-M could enhance N-induced antibody production, especially IgG2a subclass. After lymphocytes were stimulated with 10mug/ml purified N antigen, The CD4+ and CD8+ T cells of N and M plus N group were increased compared with those of control groups, and the M protein could augment the activation of lymphocytes induced by N DNA vaccine. Cytokine ELISA analysis revealed that co-injection of M could enhance the levels of IFN-gamma, IL-2 release induced by N antigen. Further experiments in field mouse also support the claim that membrane protein can augment the N-specific immune responses. Virus challenge test was conducted in BSL3 bio safety laboratory with Brandt's vole SARS-CoV model, and the results indicated that co-immunization of M and N antigens could reduce the mortality and pathological changes in lung from the virus infection.
- Published
- 2006
39. Expression of AIV Subtype H5HA, H7HA and H9HA Hemagglutinin Gene in Pichia pastoris
- Author
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Song Han, Mingxiao Ma, Kuoshi Jin, Huijun Lu, Ning-yi Jin, Yi-Ming Xu, Guo-Dong Liang, and Zhi-ping Xia
- Subjects
Influenza A Virus, H5N1 Subtype ,Influenza A Virus, H7N7 Subtype ,Hemagglutinin (influenza) ,Hemagglutinin Glycoproteins, Influenza Virus ,Biology ,medicine.disease_cause ,biology.organism_classification ,Virology ,Molecular biology ,Pichia ,Yeast ,Pichia pastoris ,Plasmid ,Antigen ,Influenza A Virus, H9N2 Subtype ,Influenza A virus ,medicine ,biology.protein ,Animals ,General Earth and Planetary Sciences ,Subunit vaccines ,Gene ,General Environmental Science - Abstract
The expression of the hemagglutinins of Avian influenza virus H5 H7and H9 subtypes was studied in this article by Pichia pastoris, one of the eukaryotis expression systems. Three reconstructed expression plasmids and engineering strains, named pPIC9K-H5HA, pPIC9K-H7HA, pPIC9K-H9HA and GS115/pPIC9K-H5HA, GS115/pPIC9K-H7HA, GS115/pPIC9K-H9HA repectively, were obtained. The reconstructed yeast engineering strains were identified by MD and MM plate selecting and PCR. The induced interests proteins were examined by SDS-PAGE and Western-bloting,the results showed that the interest genes were expressed exactly. And this will be helpful in the future study of antigen detection and antibody detection kit, as well in the subunit vaccines developing.
- Published
- 2006
40. [Study on the B cell linear epitopes of rabies virus CVS-11 nucleoprotein]
- Author
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Xin-Jun, Lv, Xin-Xin, Shen, Peng-Cheng, Yu, Hao, Li, Li-Hua, Wang, Qing, Tang, and Guo-Dong, Liang
- Subjects
Male ,Mice, Inbred BALB C ,Rabies ,Molecular Sequence Data ,Antibodies, Viral ,Mice ,Nucleoproteins ,Rabies virus ,Animals ,Epitopes, B-Lymphocyte ,Humans ,Female ,Amino Acid Sequence ,Epitope Mapping - Abstract
To study the B cell linear epitopes of rabies virus CVS-11 nucleoprotein, peptides were synthesized according to the amino acid sequences of B cell linear epitopes. Linear epitopes predicted by bioinformatics analysis were evaluated with immunological techniques. Indirect enzyme-linked immunosorbent assay showed that titers of antibodies to peptides (355-369 and 385-400 residues of rabies virus CVS-11 nucleoprotein) were above 1:12 800 in mouse sera. The antibodies recognized denatured rabies virus CVS-11 nucleoprotein in Western blot analysis. Purified anti-peptide antibodies recognized natural rabies virus CVS-11 nucleoprotein in BHK-21 cells in indirect fluorescent antibody test. The 355-369 and 385-400 residues of rabies virus CVS-11 nucleoprotein were validated as B cell linear epitopes.
- Published
- 2014
41. [First isolation of quang binh-like virus from mosquitoes in China]
- Author
-
Yun, Feng, Hong-Bin, Li, Jin, Zhu, Yu-Zhen, Zhang, Wei-Hong, Yang, Jian-Hua, Fan, Guo-Dong, Liang, and Hai-Lin, Zhang
- Subjects
Evolution, Molecular ,China ,Culicidae ,Cricetinae ,Animals ,Sequence Homology ,Insect Viruses ,Phylogeny ,Cell Line - Abstract
This study aims to investigate the distribution patterns of mosquito-borne viruses in Menghai County, Xishuangbanna Prefecture, Yunnan Province, China and to provide evidence for the prevention and control of mosquito-borne diseases. Mosquito samples were collected using mosquito lamps. Viruses were isolated from the samples by cell culture, and the isolates were identified by RT-PCR. The genomes of isolates were sequenced for phylogenetic analysis. In July 2012, a total of 1468 mosquitoes were captured in Daluo Town of Menghai County; they were divided into 32 pools, including Culex tritaeniorhynchus (28 pools, 1383 mosquitoes), Culex quinquefasciatus (2 pools, 66 mosquitoes), and Anopheles (2 pools, 19 mosquitoes). Golden hamster kidney cells (BHK-21) and Aedes albopictus cells (C6/36) were used for virus isolation. The results showed that C6/36 cells were susceptible to two isolates recovered from Culex tritaeniorhynchus (BNDL1205 and BNDL1227), with marked cytopathic effect (CPE) of cell fusion. By contrast, the two isolates could not cause CPE in BHK-21 cells. RT-PCR was performed for the two isolates using the flavivirus-specific primers FU2/cFD3, and a 800-bp amplicon was obtained from both of them. Phylogenetic analysis showed that the two isolates shared the same evolutionary branch with the Quang Binh virus (QBV) strain VN180, which had been isolated from Vietnam, with nucleotide sequence homologies of 83.4% and 82.9%, respectively. However, there existed relatively large differences in nucleotide sequence between them and other Culex flavivirus strains previously isolated in China and other regions. In light of the similarity between the two isolates and QBV, BNDL1205 and BNDL122 were referred to as Quang Binh-like virus, which were first reported in China.
- Published
- 2014
42. [Synthesis and immunosuppressive effects of novel phthalazine ketone derivatives]
- Author
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Ya-Li, Wang, Qing-He, Wang, Hong-Guang, Yang, Bo-Jun, Hao, Guo-Dong, Liang, Chong-Guo, Jiang, and Mao-Sheng, Cheng
- Subjects
Inhibitory Concentration 50 ,Mice ,Mice, Inbred BALB C ,Structure-Activity Relationship ,IMP Dehydrogenase ,T-Lymphocytes ,Animals ,Phthalazines ,Female ,Immunosuppressive Agents ,Spleen ,Cell Proliferation - Abstract
A series of phthalazine ketone compounds were synthesized and the structures were confirmed by H NMR and HR-MS spectrum. All target compounds were obtained through 7 steps, including selective reduction, nitration, bromination, ring enlargement, reduction, Knoevenagel and acylated reaction. The compounds were evaluated for their immunosuppressive effects of T-cell proliferation and inhibitory activity of IMPDH type II in vitro, as well as their structure-activity relationship were assessed. Several compounds exhibited strong immunosuppressive properties, especially compounds 7f and 7h, with IC50 values of 0.093 micromol x L(-1) and 0.14 micromol x L(-1) respectively, which were superior to mycophenolic acid. The information obtained from the studies may be useful for further research on the immunosuppressive agents.
- Published
- 2014
43. [Evolutionary and transmission characteristics of rabies viruses in Asia]
- Author
-
Xiao-Yan, Tao, Hao, Li, Qing, Tang, and Guo-Dong, Liang
- Subjects
Evolution, Molecular ,Asia ,Rabies ,Rabies virus ,Humans ,Phylogeny - Abstract
To understand the rabies virus (RABV) evolutionary relationship between the strains of China and Asia and to know the evolution and transmission characteristics of RABV in Asia.The G sequences of representative strains from China were selected and combined the sequences of other countries in Asia to analyze using BEAST and MigraPhyla software.The phylogenetic analysis showed that six groups (China I-VI) of China had different epidemic range: China I , II and V groups just cycled in our country; China VI group, from Guangxi and Yunnan provinces, crossed with Southeast Asian strains; China III group and IV group also have closer genetic relationship with Asian country strains.Geographic migration in Asia showed that Thailand and India may be two rabies transmission centers in Asia.
- Published
- 2013
44. [Construction and characterization of EGFP reporter gene labeled Sindbis virus]
- Author
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Ling-Ling, Deng, Jiang-Jiao, Li, Yan, Wei, Huan-Qin, Wang, Feng-Juan, Zhang, Ji-Guo, Sun, Chang, Chen, Wu-Yang, Zhu, and Guo-Dong, Liang
- Subjects
Base Sequence ,Genes, Reporter ,Green Fluorescent Proteins ,Molecular Sequence Data ,Sindbis Virus - Abstract
To construct and characterize EGFP reporter gene labeled Sindbis virus (SINV).The reporter gene EGFP was inserted into the genome of infectious clone pBR-XJ160 by using multi-fusion long fragment PCR method. Then apply reverse genetic manipulation technique to rescue and obtain EGFP labeled SINV.We successively obtained labeled SINV, which has good fluorescent expression characteristics and genetic stability.The labeled virus can be seen in living cells and living body, and this serves as a good tool for cell and tissue tropism and biological function study of viruses. This study laid a foundation for further studying the cell tropism, biological functions and infection mechanism of SINV.
- Published
- 2013
45. Factors influencing the number of rabies cases in children in China
- Author
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Miao, Song, Qing, Tang, Simon, Rayner, Xiao Yan, Tao, Xin Xin, Shen, and Guo Dong, Liang
- Subjects
Male ,China ,Dogs ,Adolescent ,Rabies Vaccines ,Rabies ,Child, Preschool ,Prevalence ,Animals ,Humans ,Female ,Dog Diseases ,Child - Abstract
To understand the epidemic situation and factors influencing rabies cases in children in China, we obtained an overview of the current epidemic based on individual data of rabies cases in children and a descriptive analysis was carried on the prevalence and related factors. The results showed that the rabies cases in children accounted for 21.3% of the total number of rabies cases in China, 97.0% of these cases occurred in rural areas, they were mainly caused by dogs (81.5%), and were primarily level III exposure (47.7%). More than half of the cases were not treated with wound care, vaccination rate was extremely low (15.7%), and only 5.9% of cases were injected with antibodies. Furthermore, 25.4% of cases adopted incorrect treatments such as extruding bleed and wound closure, cases vaccinated with 5 injections accounted for only 22.5%. In conclusion, the prevalence of rabies cases in children in China remains a serious concern, the number and immune status of dogs in rural areas, and knowledge of rabies by risk populations should be considered in future rabies prevention and control programs.
- Published
- 2013
46. Duration of Antibody Responses after Severe Acute Respiratory Syndrome
- Author
-
Yi Hua Chang, Tao Lan, Guo Dong Liang, Xiang Yi Tian, Lin-Fa Wang, Li Ping Wu, Nai Chang Wang, Li Yuan Zhang, Lei Zheng, and Dan Yu Na
- Subjects
Microbiology (medical) ,China ,Epidemiology ,lcsh:Medicine ,Antibodies, Viral ,Severe Acute Respiratory Syndrome ,Immunoglobulin G ,lcsh:Infectious and parasitic diseases ,Sars virus ,Medicine ,antibodies ,Humans ,lcsh:RC109-216 ,Longitudinal Studies ,Respiratory system ,skin and connective tissue diseases ,Viral immunology ,SARS ,biology ,business.industry ,lcsh:R ,fungi ,convalescent ,Dispatch ,longitudinal study ,body regions ,Titer ,Infectious Diseases ,Antibody response ,Severe acute respiratory syndrome-related coronavirus ,Immunology ,Antibody Formation ,biology.protein ,Antibody ,business ,Antibody formation - Abstract
Among 176 patients who had had severe acute respiratory syndrome (SARS), SARS-specific antibodies were maintained for an average of 2 years, and significant reduction of immunoglobulin G-positive percentage and titers occurred in the third year. Thus, SARS patients might be susceptible to reinfection >or=3 years after initial exposure.
- Published
- 2007
47. Comparative analysis of the pathogenic mechanisms of street rabies virus strains with different virulence levels
- Author
-
Jing Feng, Yin, Yu Lin, Ding, Ying, Huang, Xiao Yan, Tao, Hao, Li, Peng Cheng, Yu, Xin Xin, Shen, Wen Tao, Jiao, Guo Dong, Liang, Qing, Tang, and Feng Long, Wang
- Subjects
China ,Mice ,Mice, Inbred ICR ,Virulence ,Fluorescent Antibody Technique, Direct ,Rabies ,Rabies virus ,Animals ,Brain ,Cattle Diseases ,Cattle - Abstract
To characterize two strains of street rabies virus (RABV) isolated from the brain tissue of cattle from Inner Mongolia. Differences in the histopathological and ultrastructural changes in the brain tissue of infected mice were determined to reveal variation in the pathogenesis of infection between street rabies virus strains.Ten-day-old mice were intracranially inoculated with one of three virus strains and brain tissue harvested when the mice were moribund. Various histopathological and ultrastructural markers of disease were then compared between the groups.Infection with the street virus strain CNM1101C resulted in severe neuronal dendrites damage, but only mild cell apoptosis, T lymphocyte infiltration and microglial activation. Infection with the other street virus strain, CNM1103C, was characterized by cell apoptosis, T lymphocyte infiltration and microglial activation as well as dendrites damage. However, in comparison, infection with the attenuated virus strain CTN caused severe T lymphocyte infiltration, microglial activation and cell apoptosis, but left the neuronal dendrites intact.The two street rabies virus strains isolated from cattle from Inner Mongolia had different levels of virulence and caused distinct pathological changes in infected mice. Therefore, we concluded that different pathogenic mechanisms exist between different RABV strains.
- Published
- 2013
48. [An outbreak of imported dengue fever from Myanmar to the border of China, with its viral molecular epidemiological features]
- Author
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Hai-lin, Zhang, Shi-hong, Fu, Zhang, Deng, Jun, Yuan, Hong-yue, Jiang, Ming-hua, Li, Xiao-yan, Gao, Jing-lin, Wang, Yong-hua, Liu, Zheng-liu, Yin, Wei-hong, Yang, Yu-zhen, Zhang, Yun, Feng, Huan-yu, Wang, and Guo-dong, Liang
- Subjects
Adult ,Male ,China ,Molecular Epidemiology ,Adolescent ,Genotype ,Infant ,Myanmar ,Dengue Virus ,Middle Aged ,Dengue ,Child, Preschool ,Humans ,RNA, Viral ,Female ,Child ,Phylogeny - Abstract
To understand the epidemiologic characteristics of dengue fever, imported from Myanmar to the border of Yunnan province, China. Viral molecular epidemiologic features were also studied.Questionnaires were used on each diagnosed, suspected dengue fever, case or unknown cases with fever when coming from Myanmar entering the port and hospitals in Ruili city of Yunnan province. Serum samples of these patients were collected to detect IgM antibody against dengue virus and RT-PCR assay. Homology and phylogenetic tree based on the whole nucleotide sequence of PrM-C and NS5 gene of dengue virus were further analyzed.A total of 103 sera were collected from patients at acute stage in Ruili city in July to November 2008. Among them, 49 cases were confirmed for dengue fever according to IgM and nucleic acid testings. Except one, other 48 cases were all imported into Ruili, from Myanmar. Of those, 18 patients were residents from Mujie city of Myanmar and hospitalized in Ruili and the rest 30 patients were Chinese citizens who had finished business and returned from Myanmar. Two isolates of serum samples from the imported cases were identified and both homology and phylogenetic analysis were performed, using the nucleotide sequences of PrM and NS5 genes. They were divided into dengue type 1 (RLB61) and dengue type 3 (RLC31) and were closer to the dengue virus strains isolated from Southeast Asia countries.It is confirmed that an epidemic of dengue fever which was imported from Myanmar to Ruili city of Yunnan province, China. Evidence also showed that both type I and III epidemic strains of dengue virus did exist in Mujie city of Myanmar in 2008.
- Published
- 2013
49. Identification of animal rabies in Inner Mongolia and analysis of the etiologic characteristics
- Author
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Jing Feng, Yin, Jin Ling, Wang, Qing, Tang, Yu Lin, Ding, Xiaoyan, Tao, Hao, Li, Miao, Song, Zhenyang, Guo, Xin Xin, Shen, Guo Dong, Liang, and Feng Long, Wang
- Subjects
Acetazolamide ,Dogs ,Nucleoproteins ,Time Factors ,Rabies ,Rabies virus ,Animals ,Brain ,Cattle Diseases ,Cattle ,Dog Diseases ,Mongolia ,Phylogeny - Abstract
To perform pathological observation and etiological identification of specimens collected from dairy cows, beef cattle and dogs which were suspected of rabies in Inner Mongolia in 2011, and analyze their etiological characteristics.Pathological observation was conducted on the brain specimens of three infected animals with Hematoxylin-Eosin staining, followed by confirmation using immunofluorescence and nested RT-PCR methods. Finally, phylogenetic analysis was conducted using the virus N gene sequence amplified from three specimens.Eosinophilic and cytoplasmic inclusion bodies were seen in neuronal cells of the CNS; and rabies non-characteristic histopathological changes were also detected in the CNS. The three brain specimens were detected positive. N gene nucleotide sequence of these three isolates showed distinct sequence identity, therefore they fell into different groups in the phylogenetic analysis. N gene in the cow and dog had higher homology with that in Hebei isolate, but that in the beef cattle had higher homology with that in Mongolian lupine isolate and Russian red fox isolate.Rabies were observed in the dairy cow, beef cattle and canine in the farm in Inner Mongolia, in 2011, which led to a different etiologic characteristics of the epidemic situation.
- Published
- 2013
50. [Japanese encephalitis virus with genotype I is predominant in Sichuan Province]
- Author
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Jia-Ke, Zhang, Shi-Hua, Lin, Xing-Yu, Zhou, Dan-Lin, Chen, Shi-Hong, Fu, Xue-Cheng, Liu, Wei, Yuan, Yi-Ou, Cao, Huan-Yu, Wang, Wei, Li, Mei, Hu, and Guo-Dong, Liang
- Subjects
Encephalitis Virus, Japanese ,China ,Viral Proteins ,Culicidae ,Genotype ,Molecular Sequence Data ,Animals ,Humans ,Amino Acid Sequence ,Encephalitis, Japanese ,Sequence Alignment ,Phylogeny - Abstract
To understand molecular characteristics of Japanese encephalitis virus (JEV) isolated from the major Japanese encephalitis epidemic areas in Sichuan Province, and to provide the foundation for JEV prevention.13 JEV strains were isolated from mosquitoes in Sichuan during 2007-2010, E genes and preM genes were sequenced and phylogenetic analyses were performed using MEGA5 molecular software.Phylogenetic analysis indicated that all 13 JEV strains from Sichuan belonged to genotype I, homologies at nucleotide level and deduced amino acid level in PreM gene were 97%-100% and 98.7%-100%, and 97.8%-99.9% and 99.6%-100% in E gene, respectively. Homologies at nucleotide level and deduced amino acid level in PreM gene between 13 JEV strains and JEV isolated in 2004 in Sichuan were 96.2%-99.1% and 97.5%-98.7%, and were 97.7%-99.6% and 98. 6%-100% in E gene, respectively. By comparison with vaacine strains P3 and SA14-14-2, homologies at nucleotide level and deduced amino acid level were 84.1%-85.8% and 93.7%-96.2% in PreM gene, and were 87.6%-88.3% and 97%-97.8% in E gene, respectively. The neurovirulence-related 8 amino acid sites encode by E gene remained unchanged in 13 JEV strains.JEV with genotype I predominated in Sichuan, nucleotide sequences and deduced amino acid sequences in PreM gene and E gene were highly conserved, key neurovirulence-rerlated sites remained unchanged. It suggested currently used vaccine is still capable of preventing JEV infection.
- Published
- 2012
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