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[Study on the B cell linear epitopes of rabies virus CVS-11 nucleoprotein]
- Source :
- Bing du xue bao = Chinese journal of virology. 30(3)
- Publication Year :
- 2014
-
Abstract
- To study the B cell linear epitopes of rabies virus CVS-11 nucleoprotein, peptides were synthesized according to the amino acid sequences of B cell linear epitopes. Linear epitopes predicted by bioinformatics analysis were evaluated with immunological techniques. Indirect enzyme-linked immunosorbent assay showed that titers of antibodies to peptides (355-369 and 385-400 residues of rabies virus CVS-11 nucleoprotein) were above 1:12 800 in mouse sera. The antibodies recognized denatured rabies virus CVS-11 nucleoprotein in Western blot analysis. Purified anti-peptide antibodies recognized natural rabies virus CVS-11 nucleoprotein in BHK-21 cells in indirect fluorescent antibody test. The 355-369 and 385-400 residues of rabies virus CVS-11 nucleoprotein were validated as B cell linear epitopes.
Details
- ISSN :
- 10008721
- Volume :
- 30
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Bing du xue bao = Chinese journal of virology
- Accession number :
- edsair.pmid..........ea2f1c029b317d15888161df16d2343c