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Isolation and full-length genome analysis of mosquito-borne Manzanilla virus from Yunnan Province, China
- Source :
- BMC Research Notes
- Publication Year :
- 2015
- Publisher :
- BioMed Central, 2015.
-
Abstract
- Background There have been four strains on Manzanilla virus (MANV) identified to date. Here, we identify a novel MANV strain (DHL10M107) isolated from Culex tritaeniorhynchus Giles mosquitoes from Ruili city, Dehong prefecture, Yunnan Province, in the People’s Republic of China. Results The DHL10M107 L, M and S genes were sequenced at the nucleotide and deduced amino acid levels. The L, M and S gene sequences of DHL10M107 clustered with the MANV strains VN04-2108, TRVL3587, SA An 4165, and AV 782. DHL10M107 was most closely related to VN04-2108. Nucleotide homology ranged between 96 and 99% between DHL10M107 and VN04-2108. In terms of amino acid homology, all of the amino acid differences were in the L (96.3% homologous) and M (97.7% homologous) fragments. Conclusions DHL10M107 is likely a MANV isolated from mosquitos in the Yunnan Province. This is the first reported isolation of MANV in mainland China. Electronic supplementary material The online version of this article (doi:10.1186/s13104-015-1198-5) contains supplementary material, which is available to authorized users.
- Subjects :
- China
Orthobunyavirus
Culex
Short Report
Genome, Viral
Biology
Oya virus
Genome
General Biochemistry, Genetics and Molecular Biology
Homology (biology)
Viral Proteins
Phylogenetics
parasitic diseases
Animals
Amino Acid Sequence
Manzanilla virus
Gene
Peptide sequence
Phylogeny
Genetics
Medicine(all)
Base Sequence
Sequence Homology, Amino Acid
Biochemistry, Genetics and Molecular Biology(all)
General Medicine
biology.organism_classification
Culex tritaeniorhynchus
Subjects
Details
- Language :
- English
- ISSN :
- 17560500
- Volume :
- 8
- Database :
- OpenAIRE
- Journal :
- BMC Research Notes
- Accession number :
- edsair.doi.dedup.....260caa6ae75eeda511d7b508fc3950f6