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Expression of AIV Subtype H5HA, H7HA and H9HA Hemagglutinin Gene in Pichia pastoris
- Source :
- Chinese Journal of Biotechnology. 22:231-236
- Publication Year :
- 2006
- Publisher :
- Elsevier BV, 2006.
-
Abstract
- The expression of the hemagglutinins of Avian influenza virus H5 H7and H9 subtypes was studied in this article by Pichia pastoris, one of the eukaryotis expression systems. Three reconstructed expression plasmids and engineering strains, named pPIC9K-H5HA, pPIC9K-H7HA, pPIC9K-H9HA and GS115/pPIC9K-H5HA, GS115/pPIC9K-H7HA, GS115/pPIC9K-H9HA repectively, were obtained. The reconstructed yeast engineering strains were identified by MD and MM plate selecting and PCR. The induced interests proteins were examined by SDS-PAGE and Western-bloting,the results showed that the interest genes were expressed exactly. And this will be helpful in the future study of antigen detection and antibody detection kit, as well in the subunit vaccines developing.
- Subjects :
- Influenza A Virus, H5N1 Subtype
Influenza A Virus, H7N7 Subtype
Hemagglutinin (influenza)
Hemagglutinin Glycoproteins, Influenza Virus
Biology
medicine.disease_cause
biology.organism_classification
Virology
Molecular biology
Pichia
Yeast
Pichia pastoris
Plasmid
Antigen
Influenza A Virus, H9N2 Subtype
Influenza A virus
medicine
biology.protein
Animals
General Earth and Planetary Sciences
Subunit vaccines
Gene
General Environmental Science
Subjects
Details
- ISSN :
- 18722075
- Volume :
- 22
- Database :
- OpenAIRE
- Journal :
- Chinese Journal of Biotechnology
- Accession number :
- edsair.doi.dedup.....eeadf908cae2d737a6bcd81770f6547c