251. Impairment of reovirus mRNA ‘cap’ methylation in interferon-treated mouse L929 cells
- Author
-
Peter Lengyel and Ronald C. Desrosiers
- Subjects
viruses ,Biology ,Reoviridae ,Virus Replication ,Methylation ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Virus ,Mice ,chemistry.chemical_compound ,L Cells ,Interferon ,medicine ,Animals ,RNA, Messenger ,Mammalian orthoreovirus 3 ,Messenger RNA ,Methionine ,Methyltransferases ,Virology ,Molecular biology ,In vitro ,Uridine ,Molecular Weight ,chemistry ,RNA, Viral ,Interferons ,MRNA methylation ,medicine.drug - Abstract
Reovirus mRNAs synthesized in vitro by the virionassociated enzyme have a 5' 'cap 1' structure (m7G(5')ppp(5')GmpCp...). However, about one third to one half of the reovirus mRNAs formed in mouse L929 cells have a 5' 'cap 2' structure (m7G(5')ppp(5')GmpCmp...) and the rest have a 5' 'cap 1' structure. The finding that virus mRNA 'cap' methylation is impaired in extracts of interferon-treated cells prompted us to study the effect of interferon on virus mRNA 'cap' methylation in vivo. Using labeling with [3H]-guanosine and dual labeling with [3H]methionine and [14C]uridine we compared the 5' structures of reovirus mRNAs accumulating between 5 and 11 h after infection in: L929 cells treated with 390 to 2600 U/ml of a partially purified mouse interferon preparation and untreated L929 cells. The treatment resulted in a 70 to 98% decrease in the 24 h virus yield and in a 50 to 55% decrease in the label accumulated in virus mRNAs. The 'capping' of virus mRNAs and the methylation of their 5' terminal and adjacent G residues were not diminished in interferon-treated cells. However, the percent of 'cap 2' termini was 36 to 47% lower in virus mRNAs from interferon-treated cells than in virus mRNAs from control cells. The interferon treatment did not result in the appearance of additional methylated nucleotides in the virus mRNAs.
- Published
- 1979