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Measurement of the electrokinetic properties of vaccinia and reovirus by laser-illuminated whole-particle microelectrophoresis
- Source :
- Journal of Virological Methods. 2:251-260
- Publication Year :
- 1981
- Publisher :
- Elsevier BV, 1981.
-
Abstract
- The electrokinetics of vaccinia virus and reovirus type 3 were studied in dilute electrolyte solutions by whole-particle microelectrophoresis with dark-field laser illumination. The electrophoretic mobilities were dependent upon pH and electrolyte composition. The isoelectric point of vaccinia virus in dilute NaCl was 4.8 +/- 0.1; that of reovirus was 3.8 +/- 0.1. The mobility of reovirus was -2.65 +/- 0.30 micrometer . S-1 . V-1 . cm-1. Treatments known to cause irreversible changes in the structure of vaccinia, such as heating to 90 degrees C or exposure to pH 10, altered the virus's mobility under the reference conditions. The practical range of whole-particle microelectrophoresis thus extends to microorganisms as small as reovirus, allowing their electrokinetic and related surface properties to be studied.
- Subjects :
- Electrophoresis
Hot Temperature
Surface Properties
viruses
Vaccinia virus
Electrolyte
Sodium Chloride
Biology
Reoviridae
law.invention
Calcium Chloride
chemistry.chemical_compound
Electrokinetic phenomena
Microelectrophoresis
law
Virology
Isoelectric Point
Mammalian orthoreovirus 3
Deoxyribonucleases
Lasers
Laser
Molecular biology
Bicarbonates
Sodium Bicarbonate
Isoelectric point
chemistry
Biophysics
Particle
Laser illumination
Vaccinia
Subjects
Details
- ISSN :
- 01660934
- Volume :
- 2
- Database :
- OpenAIRE
- Journal :
- Journal of Virological Methods
- Accession number :
- edsair.doi.dedup.....a8a30e37d6cdc5a89d535a5de8b0238e