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Mechanism of interferon action studies on the mechanism of interferon-mediated inhibition of reovirus messenger RNA translation in cell-free protein synthesis systems from mouse ascites tumor cells
- Source :
- Virology. 75:166-176
- Publication Year :
- 1976
- Publisher :
- Elsevier BV, 1976.
-
Abstract
- The biochemical mechanism of the interferon-mediated inhibition of reovirus messenger RNA translation in cell-free extracts prepared from mouse ascites tumor cells was investigated. The following results were obtained: (1) Cell-free extracts from interferon-treated cells catalyze the formation of methionyl-X initiation dipeptides in response to reovirus mRNA at a rate and extent only slightly less than that of extracts from untreated cells. Under conditions where the overall translation in vitro of reovirus mRNA is inhibited by 75% or more, the formation of methionyl-X initiation dipeptides is inhibited much less, about 30%. (2) Neither methionyl-X initiation dipeptide formation nor overall translation of reovirus mRNA is significantly affected by the addition of either S -adenosyl- l -methionine or S -adenosyl- l -homocysteine to cell-free systems prepared from either interferon-treated or untreated cells. (3) Reovirus mRNA synthesized in vitro in the presence of S -adenosyl- l -methionine is translated slightly more efficiently than either reovirus mRNA synthesized in the presence of S -adenosyl- l -homocysteine or reovirus mRNA synthesized in the absence of both S -adenosyl- l -methionine and S -adenosyl- l -homocysteine by cell-free systems prepared from untreated ascites cells; by contrast, all of the reovirus mRNA preparations were translated very poorly by cell-free systems prepared from interferon-treated cells. (4) Exogenously added mouse ascites cell transfer RNA partially reverses the inhibition of viral mRNA translation in vitro catalyzed by cell-free systems prepared from interferon-treated cells in response to reovirus mRNA synthesized in the absence or in the presence of either S -adenosyl- l -methionine or S -adenosyl- l -homocysteine. Yeast and rat liver tRNA as well as tRNA from two prokaryotes, Escherichia coli and Streptococcus faecalis , do not significantly stimulate viral mRNA translation in the interferon-treated cell-free system. Translation catalyzed by untreated control extracts is not dependent upon the addition of tRNA. (5) Reovirus [ 32 P]mRNA recovered from interferon-treated cell-free protein synthesis systems after 15-min incubation possesses a profile similar to that of mRNA recovered from untreated control systems when analyzed by polyacrylamide-agarose-urea gel electrophoresis. These results indicate that the interferon-mediated inhibition of viral mRNA translation in vitro is exerted at a step of polypeptide chain biosynthesis which is subsequent to formation of the first peptide bond and which either directly or indirectly involves the participation of transfer RNA.
- Subjects :
- S-Adenosylmethionine
Biology
Reoviridae
Cell Line
chemistry.chemical_compound
RNA, Transfer
Biosynthesis
Interferon
Virology
medicine
Protein biosynthesis
RNA, Messenger
Peptide Chain Initiation, Translational
Mammalian orthoreovirus 3
Messenger RNA
Methionine
Cell-Free System
Translation (biology)
S-Adenosylhomocysteine
Molecular biology
In vitro
Biochemistry
chemistry
Protein Biosynthesis
Transfer RNA
RNA, Viral
Interferons
medicine.drug
Subjects
Details
- ISSN :
- 00426822
- Volume :
- 75
- Database :
- OpenAIRE
- Journal :
- Virology
- Accession number :
- edsair.doi.dedup.....8be220dc59032dbba41a62558bfcaf33