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Second-step concentration of viruses in drinking and surface waters using polyethylene glycol hydroextraction
- Source :
- Canadian Journal of Microbiology. 25:587-592
- Publication Year :
- 1979
- Publisher :
- Canadian Science Publishing, 1979.
-
Abstract
- In our laboratory, virus adsorbed to talc–Celite layers is eluted with 100 mL of 10% fetal calf serum (FCS) in normal saline (pH 9.0). A further 10-fold reduction in the volume of the eluate was necessary before its inoculation into cell cultures.A 100-mL volume of an experimentally contaminated sample was placed in a dialysis sac and hydroextracted overnight (4 °C) with polyethylene glycol (PEG) 6000. The viscous material remaining in the sac was resuspended in 10 mL of Earle's balanced salt solution. After membrane filtration (0.2 μm), the concentrate was plaque assayed in BS-C-1 cells. Using this technique, recoveries of five laboratory-adapted enteric viruses (polio 1, echo 6, coxsackie B5, coxsackie A9, and reo 3) and four freshly isolated enteric virus strains (polio 1, echo 1, coxsackie B3, and reo) ranged from 87 to 97%. In comparative tests, PEG hydroextraction was simpler and superior to organic flocculation.
- Subjects :
- Microbiological Techniques
medicine.medical_treatment
Immunology
Fresh Water
Viral Plaque Assay
Polyethylene glycol
Reoviridae
Applied Microbiology and Biotechnology
Microbiology
Polyethylene Glycols
chemistry.chemical_compound
Adsorption
Water Supply
Genetics
medicine
Centrifugation
Mammalian orthoreovirus 3
Molecular Biology
Saline
Enterovirus
Chromatography
Elution
Water Pollution
Flocculation
General Medicine
Polyethylene
Volume (thermodynamics)
chemistry
Water Microbiology
Subjects
Details
- ISSN :
- 14803275 and 00084166
- Volume :
- 25
- Database :
- OpenAIRE
- Journal :
- Canadian Journal of Microbiology
- Accession number :
- edsair.doi.dedup.....60cd7ff2e565f50a015125576f1a1ccb