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Identification of idiotypic receptors on reovirus-specific cytolytic T cells
- Source :
- Proceedings of the National Academy of Sciences. 79:7479-7483
- Publication Year :
- 1982
- Publisher :
- Proceedings of the National Academy of Sciences, 1982.
-
Abstract
- Cytolytic T lymphocytes (Tc) specific for cells infected with reovirus type 3 were shown to lyse an uninfected B-cell hybridoma line (designated 87.92.6). This hybridoma expresses and secretes an anti-idiotypic antibody that reacts with a monoclonal antibody (termed G-5). G-5 recognizes a domain on the hemagglutinin of the reovirus that is relevant to virus tropism. The Tc cell response was H-2 restricted and could be inhibited by G-5. As shown by limiting dilution analysis, identical clones lysed reovirus-infected and anti-idiotype-bearing target cells. Tc cells induced by a variant of wild-type reovirus type 3 (immunologically selected by resistance to neutralization by G-5) were unable to recognize the anti-idiotype-bearing cells although they lysed reovirus type 3-infected tumor cells. We conclude that Tc cells must bear determinants that bind anti-idiotype molecules and, furthermore, that B cells and cytolytic T cells can share these idiotypic determinants.
- Subjects :
- medicine.drug_class
viruses
Receptors, Antigen, T-Cell
Antibodies, Viral
Reoviridae
Monoclonal antibody
Virus
Cell Line
Mice
Immunoglobulin Idiotypes
medicine
Animals
Cytotoxic T cell
Receptors, Immunologic
Mammalian orthoreovirus 3
Hybridomas
Multidisciplinary
biology
H-2 Antigens
Hemagglutinin
Virology
Molecular biology
Cytolysis
Cell culture
Antigens, Surface
biology.protein
Antibody
T-Lymphocytes, Cytotoxic
Research Article
Subjects
Details
- ISSN :
- 10916490 and 00278424
- Volume :
- 79
- Database :
- OpenAIRE
- Journal :
- Proceedings of the National Academy of Sciences
- Accession number :
- edsair.doi.dedup.....1e8f6de3842c73052f622d10143c280d