171 results on '"Long Ji"'
Search Results
2. Characterization of circulating microRNA expression in patients with a ventricular septal defect.
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Dong Li, Long Ji, Lianbo Liu, Yizhi Liu, Haifeng Hou, Kunkun Yu, Qiang Sun, and Zhongtang Zhao
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Medicine ,Science - Abstract
Ventricular septal defect (VSD), one of the most common types of congenital heart disease (CHD), results from a combination of environmental and genetic factors. Recent studies demonstrated that microRNAs (miRNAs) are involved in development of CHD. This study was to characterize the expression of miRNAs that might be involved in the development or reflect the consequences of VSD.MiRNA microarray analysis and reverse transcription-polymerase chain reaction (RT-PCR) were employed to determine the miRNA expression profile from 3 patients with VSD and 3 VSD-free controls. 3 target gene databases were employed to predict the target genes of differentially expressed miRNAs. miRNAs that were generally consensus across the three databases were selected and then independently validated using real time PCR in plasma samples from 20 VSD patients and 15 VSD-free controls. Target genes of validated 8 miRNAs were predicted using bioinformatic methods.36 differentially expressed miRNAs were found in the patients with VSD and the VSD-free controls. Compared with VSD-free controls, expression of 15 miRNAs were up-regulated and 21 miRNAs were downregulated in the VSD group. 15 miRNAs were selected based on database analysis results and expression levels of 8 miRNAs were validated. The results of the real time PCR were consistent with those of the microarray analysis. Gene ontology analysis indicated that the top target genes were mainly related to cardiac right ventricle morphogenesis. NOTCH1, HAND1, ZFPM2, and GATA3 were predicted as targets of hsa-let-7e-5p, hsa-miR-222-3p and hsa-miR-433.We report for the first time the circulating miRNA profile for patients with VSD and showed that 7 miRNAs were downregulated and 1 upregulated when matched to VSD-free controls. Analysis revealed target genes involved in cardiac development were probably regulated by these miRNAs.
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- 2014
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3. Analyzing risk factors for second malignancies in early gastric carcinoma from the SEER database
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Lei Song, Fei Zhao, Lijing Zhang, Zhifang Zhao, Long Jin, Yu Zhao, and Jin Zhao
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Second primary malignancies ,Nomogram ,Competitive proportional hazard model ,Gastric carcinoma ,Harrell concordance index ,Medicine ,Science - Abstract
Abstract This retrospective study analyzed a large population of gastric cancer (GC) patients treated between 2010 and 2015 to investigate the clinical features and predictive risk factors for developing secondary primary malignancies (SPMs). The cumulative incidence of SPM was assessed using Kaplan–Meier analysis. Competing risk analyses adjusted for mortality were conducted using stratified Cox proportional hazard regression models and multivariate analyses to identify independent predictors of SPM. A total of 3289 out of 167,747 GC patients were included in the analytic cohort, with 155 patients diagnosed with SPM. Patients whose histologic type other than adenocarcinomas (AC) and signet ring cell carcinoma (SRCC) emerged as an independent risk factor for developing SPM (hazard ratio [HR] 2.262, 95% confidence interval [CI] 1.146–4.465, P = 0.019) in multivariate Cox regression analysis. The surgical method, including biopsy/local excision (HR 2.3, [CI] 1.291–4.095, P = 0.005) and subtotal/total resection ([HR] 1.947, [CI] 1.028–3.687, P = 0.041), chemotherapy ([HR] 1.527, [CI] 1.006–2.316, P = 0.047), and histologic type ([HR] 2.318, [CI] 1.193–4.504, P = 0.013)), were identified as independent risk factors in the competitive risk model. Subgroup analyses, stratified by chemotherapy, revealed an increased risk of SPM among older patients. Furthermore, a nomogram was developed and internally validated to predict the cumulative incidence of SPM in GC patients (C-index = 0.73 for 72 months). These findings suggested that in specific histologic types of GC, the lymph node infiltration region missed after local surgical resection, and concomitant chemotherapy would have an increased risk of SPM for cancer survivors.
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- 2024
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4. The association of metabolic syndrome and cognitive impairment in Jidong of China: a cross-sectional study
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Jian Lv, Xiaohui Wang, Xueyu Chen, Dong Li, Guoyong Ding, Yanru Chen, Long Ji, and Zhaoyang Tang
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Adult ,Male ,China ,medicine.medical_specialty ,Cross-sectional study ,Epidemiology ,Endocrinology, Diabetes and Metabolism ,Population ,lcsh:Diseases of the endocrine glands. Clinical endocrinology ,Cohort Studies ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,Diabetes mellitus ,medicine ,Humans ,Cognitive Dysfunction ,Longitudinal Studies ,030212 general & internal medicine ,Risk factor ,education ,Abdominal obesity ,education.field_of_study ,lcsh:RC648-665 ,business.industry ,Confounding ,General Medicine ,Middle Aged ,Mental Status and Dementia Tests ,medicine.disease ,Metabolic syndrome ,Cross-Sectional Studies ,Cognitive impairment ,Obesity, Abdominal ,Hypertension ,Blood pressure ,Female ,medicine.symptom ,business ,030217 neurology & neurosurgery ,Research Article - Abstract
Background Metabolic syndrome (Mets) is prevalent in the general population and has been reported to be an independent risk factor for cognitive impairment. This study aimed to investigate the association of Mets with the risk of cognitive impairment. Methods We studied 5854 participants from the Jidong community. Cognitive function was assessed by the Mini-Mental State of Examination (MMSE) scale. Mets was diagnosed according to the International Diabetes Federation criteria. We used logistic regression analysis to investigate the association of metabolic syndrome with the risk of cognitive impairment. Result Among the 5854 adults included in the study, the age mean (SD) of age was 44 (13.57) years, and 2916 (50.34%) were male. There was a higher (56.03%) cognitive impairment incidence rate among participants with Mets than among those without Mets. In addition, there was a significant association between Mets and cognitive impairment (OR: 2.39, 95% CI: 2.00–2.86, P P P P Conclusion Our study suggested that Mets was associated with cognitive impairment and that abdominal obesity and hypertension were associated with an increased risk of cognitive impairment.
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- 2021
5. Dynamics of single-nuclei transcriptomic profiling of adipose tissue from diverse anatomical locations during mouse aging process
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Yujie Wu, Ying Sun, Long Chen, Xingyan Tong, Can Liu, Lu Lu, Rui Zhang, Siyuan Wang, Ziyu Chen, Jiaman Zhang, Ziyin Han, Bo Zeng, Mingzhou Li, and Long Jin
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Medicine ,Science - Abstract
Abstract Adipose tissue plays critical roles in an individual’s aging process. In this research, we use single-nucleus RNA sequencing to create highly detailed transcriptional maps of subcutaneous adipose tissue and visceral adipose tissue in young and aged mice. We comprehensively identify the various cell types within the white adipose tissue of mice, our study has elucidated seven distinct cell types within this tissue. Further analyses focus on adipocytes, fibro-adipogenic progenitors, and immune cells, revealing age-related declines in the synthetic metabolic activity of adipocytes, diminished immune regulation, and reduced maturation or proliferation of fibroblasts in undifferentiated adipocytes. We confirm the presence of distinct subpopulations of adipocytes, highlighting decreases in adipogenesis subgroups due to aging. Additionally, we uncover a reduction in immune cell subpopulations, driven by age-associated immune system dysregulation. Furthermore, pseudo-time analyses indicate that Adipocyte1 represents the 'nascent' phase of adipocyte development, while Adipocyte2 represents the 'mature' phase. We use cell–cell interaction to explore the age-dependent complexities of the interactions between FAPs and adipocytes, and observed increased expression of the inflammation-related Retn-Tlr4 interaction in older mice, while the anti-inflammatory Angpt1-Tek interaction was only detected in young mice. These transcriptional profiles serve as a valuable resource for understanding the functional genomics underlying metabolic disorders associated with aging in human adipose tissue.
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- 2024
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6. Elevated Plasmin(ogen) as a Common Risk Factor for COVID-19 Susceptibility
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Runzhen Zhao, Michael A. Matthay, Hong Long Ji, and Sadis Matalon
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0301 basic medicine ,Proteases ,Physiology ,Plasmin ,Estrone ,medicine.medical_treatment ,Pneumonia, Viral ,Review ,030204 cardiovascular system & hematology ,03 medical and health sciences ,Betacoronavirus ,0302 clinical medicine ,Risk Factors ,Physiology (medical) ,Diabetes mellitus ,Fibrinolysis ,medicine ,Humans ,Fibrinolysin ,Risk factor ,Molecular Biology ,Furin ,Pandemics ,Infectivity ,biology ,business.industry ,SARS-CoV-2 ,COVID-19 ,General Medicine ,medicine.disease ,Hyperfibrinolysis ,Hospitalization ,comorbidity ,030104 developmental biology ,Immunology ,biology.protein ,plasmin(ogen) ,fibrinolysis ,business ,Coronavirus Infections ,medicine.drug - Abstract
Patients with hypertension, diabetes, coronary heart disease, cerebrovascular illness, chronic obstructive pulmonary disease, and kidney dysfunction have worse clinical outcomes when infected with SARS-CoV-2, for unknown reasons. The purpose of this review is to summarize the evidence for the existence of elevated plasmin(ogen) in COVID-19 patients with these comorbid conditions. Plasmin, and other proteases, may cleave a newly inserted furin site in the S protein of SARS-CoV-2, extracellularly, which increases its infectivity and virulence. Hyperfibrinolysis associated with plasmin leads to elevated D-dimer in severe patients. The plasmin(ogen) system may prove a promising therapeutic target for combating COVID-19.
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- 2020
7. Association between Serum Uric Acid and Non-Alcoholic Fatty Liver Disease according to Different Menstrual Status Groups
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Yuejin Li, Yong Zhou, Huamin Liu, Xiaohui Wang, Qiuping Huang, Xueyu Chen, Long Ji, Ping Ai, Xia Feng, Xizhu Xu, Yanru Chen, Dong Li, and Guoyong Ding
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Adult ,medicine.medical_specialty ,Article Subject ,Cross-sectional study ,Physical examination ,Logistic regression ,Non-alcoholic Fatty Liver Disease ,medicine ,Humans ,lcsh:RC799-869 ,Hepatology ,medicine.diagnostic_test ,Obstetrics ,business.industry ,Fatty liver ,Gastroenterology ,nutritional and metabolic diseases ,General Medicine ,Odds ratio ,Middle Aged ,medicine.disease ,digestive system diseases ,Confidence interval ,Menstruation ,Perimenopause ,Uric Acid ,Postmenopause ,Cross-Sectional Studies ,Logistic Models ,Quartile ,Abdominal ultrasonography ,lcsh:Diseases of the digestive system. Gastroenterology ,Female ,business ,Research Article - Abstract
Objective. The present study aimed to explore the association between SUA and NAFLD in women with different menstrual statuses. Methods. A total of 6043 women were selected from the Jidong and Kailuan communities for inclusion in the present study. The SUA levels of participants were divided into quartiles. NAFLD was determined by abdominal ultrasonography. Data from laboratory tests and clinical examination were collected, and basic information was obtained from standardized questionnaires. The menstrual status was stratified into menstrual period, menopause transition period, and postmenopause. Multivariate logistic regression models were used to determine the relationship between menstrual status, SUA, and NAFLD. Results. The levels of SUA in subjects with NAFLD in the menstrual period, menopause transition period, and postmenopause were 268.0 ± 71.1, 265.6 ± 67.8, and 286.7 ± 75.8 (mmol/L), respectively, and were higher than those in subjects without NAFLD. The adjusted odds ratios (ORs) with 95% confidence interval (CI) for NAFLD among participants in the menopause transition period and postmenopausal period were 1.10 (0.89–1.37) and 1.28 (1.04–1.58), respectively, compared with the menstrual period women. Compared to the lowest quartile of SUA, the adjusted ORs with 95% CI of the highest quartile for NAFLD were 2.24 (1.69–2.99) for females in the menstrual period, 1.92 (1.10–3.37) for females in the menopause transition period, and 1.47 (1.06–2.03) for females in postmenopause. Conclusions. Menstrual status was significantly correlated with NAFLD. High levels of SUA were associated with NAFLD in females during the three menstrual periods.
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- 2019
8. High Expression of C1ORF112 Predicts a Poor Outcome: A Potential Target for the Treatment of Low-Grade Gliomas
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Huan Yang, Shu-Hui Chen, Tianzhu Lu, Chen Junjun, Yu-Long Ji, Zhen Chen, Kai Yu, Huaizhen Liang, Li-chong Wang, Zi-long Tan, Xiaoli Shen, Qiaoli Lv, Wei He, and Zhe Zhang
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Oncology ,medicine.medical_specialty ,Multivariate analysis ,C1ORF112 ,low-grade glioma ,business.industry ,immunoinfiltration ,QH426-470 ,medicine.disease ,Genome ,Primary tumor ,Confidence interval ,Glioma ,Internal medicine ,Genetics ,Molecular Medicine ,Biomarker (medicine) ,Medicine ,biomarker ,prognosis ,business ,Gene ,Genetics (clinical) ,Survival analysis ,Original Research - Abstract
Background: Glioma is the most common primary tumor of the central nervous system and is associated with poor overall survival, creating an urgent need to identify survival-associated biomarkers. C1ORF112, an alpha-helical protein, is overexpressed in some cancers; however, its prognostic role has not yet been explored in gliomas. Thus, in this study, we attempted to address this by determining the prognostic value and potential function of C1ORF112 in low-grade gliomas (LGGs).Methods: The expression of C1ORF112 in normal and tumor tissues was analyzed using data from The Cancer Genome Atlas (TCGA), Chinese Glioma Genome Atlas (CGGA), Oncomine, and Rembrandt databases. The genetic changes of C1ORF112 in LGG were analyzed using cBioPortal. Survival analysis was used to evaluate the relationship between C1ORF112 expression and survival in patients with LGG. Correlation between immune infiltration and C1ORF112 expression was determined using Timer software. Additionally, data from three online platforms were integrated to identify the co-expressed genes of C1ORF112. The potential biological functions of C1ORF112 were investigated by enrichment analysis.Results: C1ORF112 mRNA was highly expressed in LGGs (p < 0.01). Area under the ROC curve (AUC) showed that the expression of C1ORF112 in LGG was 0.673 (95% confidence interval [CI] = 0.618–0.728). Kaplan-Meier survival analysis showed that patients with high C1ORF112 expression had lower OS than patients with low C1ORF112 expression (p < 0.05). Multivariate analysis showed that high expression of C1ORF112 was an independent prognostic factor for the overall survival in patients from TCGA and CGGA databases. C1ORF112 expression was positively correlated with six immunoinfiltrating cells (all p < 0.001). The enrichment analysis suggested the enrichment of C1ORF112 and co-expressed genes in cell cycle and DNA replication.Conclusion: This study suggested that C1ORF112 may be a prognostic biomarker and a potential immunotherapeutic target for LGG.
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- 2021
9. Optimization of magnetic coupling mechanism of dynamic wireless power transfer based on NSGA-II algorithm
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Weihang Tang, Long Jing, Wanyu Cao, Wenzheng Xu, Xuezhi Wu, and Hongbin Liao
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Medicine ,Science - Abstract
Abstract Optimization of magnetic coupling mechanism is an important way to improve the performance of a dynamic wireless power transfer system. Inspired by the common radial magnetic core for circular coils, a new radial magnetic core for rectangular coils is adopt. Through simulation and experimental results comparison, which has higher coupling coefficient with the same core area. Combined with the magnetic circuit analysis, the magnetic flux leakage and conduction regions are divided into magnetic fluxes with different shapes, which magnetic resistances are calculated respectively. Based on the simulation results, parameter distributions of fluxes under different conditions are obtained. Therefore, the expressions of the coupling coefficient k of the adopt magnetic cores and coils and the design parameters of coils and cores are obtained. Taking the maximum k and the minimum rate of change of coupling coefficient with 100 mm displacement as the optimization objectives, a multi-objective optimization solution is carried out by using NSGA-II algorithm. The coil optimization scheme is obtained and verified by experiments. k and Δk are 0.442 and 6.8% respectively, and the errors are less than 5%. In the optimization process, there is no simulation model constructed. The optimization modeling combined of magnetic field segmentation method and parameter fitting has lower complexity and calculation time of optimization.
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- 2024
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10. Association Between Gamma-Glutamyl Transferase, Total Bilirubin and Systemic Lupus Erythematosus in Chinese Women
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Wenran Zhang, Zhaoyang Tang, Yanjun Shi, Long Ji, Xueyu Chen, Yanru Chen, Xiaohui Wang, Meng Wang, Wei Wang, and Dong Li
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030204 cardiovascular system & hematology ,Logistic regression ,Severity of Illness Index ,Gastroenterology ,Pathogenesis ,chemistry.chemical_compound ,0302 clinical medicine ,systemic lupus erythematosus ,Lupus Erythematosus, Systemic ,Immunology and Allergy ,skin and connective tissue diseases ,Original Research ,gamma-Glutamyltransferase ,Middle Aged ,total bilirubin ,Prognosis ,Quartile ,Disease Progression ,Cytokines ,Female ,Inflammation Mediators ,Adult ,China ,medicine.medical_specialty ,Bilirubin ,case-control study ,Immunology ,digestive system ,female population ,03 medical and health sciences ,Sex Factors ,Internal medicine ,medicine ,Humans ,Risk factor ,030203 arthritis & rheumatology ,business.industry ,diagnostic marker ,Case-control study ,RC581-607 ,Rheumatology ,digestive system diseases ,ROC Curve ,chemistry ,Case-Control Studies ,gamma-glutamyl transferase ,Immunologic diseases. Allergy ,business ,Biomarkers ,TBIL - Abstract
BackgroundSystemic lupus erythematosus (SLE) affects many organs and systems of the human organism, at present, its specific pathogenesis is not completely clear, but inflammation is considered to be an important factor involved in the pathogenesis and progression of SLE. Gamma-glutamyl transpeptidase (GGT) and total bilirubin (TBIL) have different effects on inflammation: GGT has pro-inflammatory effects, on the contrary, TBIL has anti-inflammatory effects. Study has found that GGT and TBIL play opposite roles in metabolic diseases. However, the roles of them in SLE are unknown. Meanwhile, the relationship between GGT and SLE also remains unexplored.MethodWe recruited 341 SLE patients and 332 healthy individuals in Liaocheng People’s Hospital from August 2018 to May 2019. We diagnosed SLE using 2019 revised American College of Rheumatology (ACR) SLE criteria, and modeled the study outcomes using logistic regression to explore the respective relationship between GGT, TBIL and SLE. We also analyzed the interaction of GGT and TBIL in the progression of SLE.ResultsWe found that the levels of CRP, IL-6 and TNF-α in the aggravated group were significantly higher than those in the unaggravated group, the levels of C3 and C4 in the aggravated group were significantly lower than those in the unaggravated group. According to Spearman correlation analysis, GGT is proportional to CRP (rs=0.417) and IL-6 (rs=0.412), inversely proportional to C3 (rs=-0.177) and C4 (rs=0.-132). TBIL was inversely proportional to CRP (rs=-0.328) and TNF(rs=-0.360), and positively proportional to C3 (rs=0.174) and C4 (rs=0.172). In the fully adjusted model, compared to the lowest quartile, the highest quartile of GGT exhibited a positive association with the risk of SLE aggravation (OR=2.99, 95% CI: 1.42–6.31, PPP for interaction COMBINED=0.711) of high GGT level and TBIL were higher than their respective values (AUCGGT=0.612, AUCTBIL=0.614).ConclusionWe found that the effects of GGT and TBIL in the progression of SLE are opposite. High GGT level might be a risk factor for SLE aggravation, as GGT levels increased, so did the risk of SLE aggravation. At the same time, we found that low TBIL level might be a risk factor for SLE aggravation. Moreover, high GGT level and low TBIL level had a subadditive effect on the increased risk of SLE aggravation.
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- 2021
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11. Associations of D-Dimer on Admission and Clinical Features of COVID-19 Patients: A Systematic Review, Meta-Analysis, and Meta-Regression
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Runzhen Zhao, Zhenlei Su, Andrey A. Komissarov, Shan-Lu Liu, Guohua Yi, Steven Idell, Michael A. Matthay, and Hong-Long Ji
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medicine.medical_specialty ,medicine.medical_treatment ,Immunology ,030204 cardiovascular system & hematology ,Severity of Illness Index ,Fibrin Fibrinogen Degradation Products ,03 medical and health sciences ,Patient Admission ,0302 clinical medicine ,Interquartile range ,Internal medicine ,Fibrinolysis ,D-dimer ,meta-regression ,Humans ,Medicine ,Immunology and Allergy ,Meta-regression ,030212 general & internal medicine ,Diagnostic Tests, Routine ,SARS-CoV-2 ,business.industry ,Confounding ,COVID-19 ,Publication bias ,RC581-607 ,fibrinogenolysis ,comorbidity ,Meta-analysis ,Disease Progression ,Biomarker (medicine) ,fibrinolysis ,Systematic Review ,Immunologic diseases. Allergy ,business ,Biomarkers - Abstract
BackgroundDynamic D-dimer level is a key biomarker for the severity and mortality of COVID-19 (coronavirus disease 2019). How aberrant fibrinolysis influences the clinical progression of COVID-19 presents a clinicopathological dilemma challenging intensivists.MethodsWe performed meta-analysis and meta regression to analyze the associations of plasma D-dimer with 106 clinical variables to identify a panoramic view of the derangements of fibrinolysis in 14,862 patients of 42 studies. There were no limitations of age, gender, race, and country. Raw data of each group were extracted separately by two investigators. Individual data of case series, median and interquartile range, and ranges of median or mean were converted to SDM (standard deviation of mean).FindingsThe weighted mean difference of D-dimer was 0.97 µg/mL (95% CI 0.65, 1.29) between mild and severe groups, as shown by meta-analysis. Publication bias was significant. Meta-regression identified 58 of 106 clinical variables were associated with plasma D-dimer levels. Of these, 11 readouts were negatively related to the level of plasma D-dimer. Further, age and gender were confounding factors. There were 22 variables independently correlated with the D-dimer level, including respiratory rate, dyspnea plasma K+, glucose, SpO2, BUN (blood urea nitrogen), bilirubin, ALT (alanine aminotransferase), AST (aspartate aminotransferase), systolic blood pressure, and CK (creatine kinase).InterpretationThese findings support elevated D-dimer as an independent predictor for both mortality and complications. The identified D-dimer-associated clinical variables draw a landscape integrating the aggregate effects of systemically suppressive and pulmonary hyperactive derangements of fibrinolysis, and the D-dimer-associated clinical biomarkers, and conceptually parameters could be combined for risk stratification, potentially for tracking thrombolytic therapy or alternative interventions.
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- 2021
12. Artificial intelligence-assisted quantitative CT parameters in predicting the degree of risk of solitary pulmonary nodules
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Long Jiang, Yang Zhou, Wang Miao, Hongda Zhu, Ningyuan Zou, Yu Tian, Hanbo Pan, Weiqiu Jin, Jia Huang, and Qingquan Luo
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Artificial intelligence ,prediction ,lung cancer ,Medicine - Abstract
Introduction Artificial intelligence (AI) shows promise for evaluating solitary pulmonary nodules (SPNs) on computed tomography (CT). Accurately determining cancer invasiveness can guide treatment. We aimed to investigate quantitative CT parameters for invasiveness prediction.Methods Patients with stage 0–IB NSCLC after surgical resection were retrospectively analysed. Preoperative CTs were evaluated with specialized software for nodule segmentation and CT quantification. Pathology was the reference for invasiveness. Univariate and multivariate logistic regression assessed predictors of high-risk SPN.Results Three hundred and fifty-five SPN were included. On multivariate analysis, CT value mean and nodule type (ground glass opacity vs. solid) were independent predictors of high-risk SPN. The area under the curve (AUC) was 0.811 for identifying high-risk nodules.Conclusions Quantitative CT measures and nodule type correlated with invasiveness. Software-based CT assessment shows potential for noninvasive prediction to guide extent of resection. Further prospective validation is needed, including comparison with benign nodules.
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- 2024
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13. Body Mass Index and the Risk of Rheumatoid Arthritis: An Updated Dose-Response Meta-Analysis
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Yuejin Li, Dong Li, Xizhu Xu, Huamin Liu, Youxin Wang, Wei Wang, Xuezhen Liu, Haifeng Hou, Long Ji, Yanjun Shi, and Xia Feng
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Male ,0301 basic medicine ,medicine.medical_specialty ,lcsh:Medicine ,Arthritis ,Review Article ,Overweight ,General Biochemistry, Genetics and Molecular Biology ,Body Mass Index ,Arthritis, Rheumatoid ,03 medical and health sciences ,0302 clinical medicine ,Risk Factors ,Internal medicine ,Humans ,Medicine ,Obesity ,030203 arthritis & rheumatology ,General Immunology and Microbiology ,business.industry ,lcsh:R ,General Medicine ,Publication bias ,medicine.disease ,030104 developmental biology ,Relative risk ,Rheumatoid arthritis ,Meta-analysis ,Female ,medicine.symptom ,business ,Body mass index ,Cohort study - Abstract
Background. Extensive studies have been carried out to investigate the association between obesity and the risk of rheumatoid arthritis (RA); however, the results of the current reported original studies remain inconsistent. This study aimed to clarify the relationship between body mass index and rheumatoid arthritis by conducting an updated overall and dose-response meta-analysis. Methods. The relevant literature was searched using the PubMed and Embase databases (through 20 September 2018) to identify all eligible published studies. Random-effect models and dose-response meta-analyses were used to estimate the pooled risk ratio (RR) with a 95% confidence interval (CI). Subgroup analyses were also conducted based on the characteristics of the participants. Sensitivity analyses and publication bias tests were also performed to explore potential heterogeneity and bias in the meta-analysis. Results. Sixteen studies that included a total of 406,584 participants were included in the meta-analysis. Compared to participants with normal weight, the pooled RRs of rheumatoid arthritis were 1.12 (95% CI, 1.04-1.20) in overweight and 1.23 (95% CI, 1.09-1.39) in obese participants. There was evidence of a nonlinear relationship between body mass index (BMI) and RA (P for nonlinearity less than 0.001 in the overall meta-analysis, P for nonlinearity=0.025 in the case-control studies, P for nonlinearity=0.0029 in the cohort studies). No significant heterogeneity was found among studies (I2=10.9% for overweight and I2=45.5% for obesity). Conclusion. The overall and dose-response meta-analysis showed that increased BMI was associated with an increased risk for rheumatoid arthritis, which might present a prevention strategy for the prevention or control of rheumatoid arthritis. The nonlinear relationship between BMI and RA might present a personal prevention strategy for RA.
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- 2019
14. Ideal cardiovascular health metrics and the risk of non‐alcoholic fatty liver disease: A cross‐sectional study in northern China
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Xueyu Chen, Long Ji, Youxin Wang, Yanru Chen, Huamin Liu, Xia Feng, Xuezhen Liu, Yan Yao, Shasha Guo, Yong Zhou, Dong Li, Zhongni Liu, and Likun Ma
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Adult ,Blood Glucose ,Male ,China ,medicine.medical_specialty ,Adolescent ,Cross-sectional study ,Health Behavior ,Blood Pressure ,Logistic regression ,Cardiovascular System ,Body Mass Index ,Young Adult ,03 medical and health sciences ,0302 clinical medicine ,Non-alcoholic Fatty Liver Disease ,Internal medicine ,Health Status Indicators ,Humans ,Medicine ,Exercise ,Aged ,Aged, 80 and over ,Hepatology ,medicine.diagnostic_test ,business.industry ,Smoking ,Fatty liver ,Fasting ,Odds ratio ,Middle Aged ,medicine.disease ,Diet ,Cholesterol ,Cross-Sectional Studies ,Logistic Models ,Blood pressure ,Quartile ,030220 oncology & carcinogenesis ,Abdominal ultrasonography ,Female ,030211 gastroenterology & hepatology ,business ,Body mass index - Abstract
BACKGROUND AND PURPOSE The components of ideal cardiovascular health (CVH) metrics have been shown to be associated with non-alcoholic fatty liver disease (NAFLD). The present study aimed to determine the association between ideal CVH metrics and NAFLD. METHODS A total of 10,511 participants (47.26% men) aged 18 to 92 years were selected from the Jidong and Kailuan communities. Ideal CVH was based on 7 ideal CVH metrics: smoking, body mass index (BMI), physical activity, diet, total cholesterol, blood pressure and fasting blood glucose. NAFLD was determined by abdominal ultrasonography. All participants underwent questionnaire assessments and clinical and laboratory examinations. Logistic regression models were used to analyse the relationship of CVH metrics and the number of ideal CVH metrics with NAFLD. RESULTS The prevalence rates of NAFLD by CVH summary score quartiles were 64.38% (2,015/3,130), 50.16% (786/1,567), 33.28% (1,194/3,588) and 20.89% (465/2,226). Participants in the highest quartile showed a lower odds ratio (OR) than those in the lowest quartile (fully adjusted OR: 0.17, 95% CI: 0.17-0.20, P
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- 2019
15. Concise Review: Therapeutic Potential of the Mesenchymal Stem Cell Derived Secretome and Extracellular Vesicles for Radiation‐Induced Lung Injury: Progress and Hypotheses
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Cong Liu, Hong-Long Ji, and Siguang Xu
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0301 basic medicine ,Inflammation ,Cell‐Based Drug Development, Screening, and Toxicology ,Lung injury ,Exosomes ,Exosome ,03 medical and health sciences ,Paracrine signalling ,Extracellular Vesicles ,0302 clinical medicine ,medicine ,Animals ,Humans ,Regeneration ,Radiation Injuries ,Secretome ,business.industry ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,Cell Biology ,General Medicine ,Lung Injury ,medicine.disease ,Microvesicles ,030104 developmental biology ,Radiation-induced lung injury ,Cancer research ,Disease Progression ,Lung fibrosis ,Radiation pneumonitis ,Stem cell ,medicine.symptom ,business ,030217 neurology & neurosurgery ,Developmental Biology - Abstract
Radiation-induced lung injury (RILI) is a common complication in radiotherapy of thoracic tumors and limits the therapeutic dose of radiation that can be given to effectively control tumors. RILI develops through a complex pathological process, resulting in induction and activation of various cytokines, infiltration by inflammatory cells, cytokine-induced activation of fibroblasts, and subsequent tissue remodeling by activated fibroblasts, ultimately leading to impaired lung function and respiratory failure. Increasing evidence shows that mesenchymal stem cells (MSCs) may play a main role in modulating inflammation and immune responses, promoting survival and repair of damaged resident cells and enhancing regeneration of damaged tissue through soluble paracrine factors and therapeutic extracellular vesicles. Therefore, the use of the MSC-derived secretome and exosomes holds promising potential for RILI therapy. Here, we review recent progress on the potential mechanisms of MSC therapy for RILI, with an emphasis on soluble paracrine factors of MSCs. Hypotheses on how MSC derived exosomes or MSC-released exosomal miRNAs could attenuate RILI are also proposed. Problems and translational challenges of the therapies based on the MSC-derived secretome and exosomes are further summarized and underline the need for caution on rapid clinical translation. Stem Cells Translational Medicine 2019;8:344–354
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- 2019
16. Inhaled TRIM72 Protein Protects Ventilation Injury to the Lung through Injury-guided Cell Repair
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Nagaraja N. Nagre, John M. Schreiber, Seth Warren, Xiaofei Cong, Rolf D. Hubmayr, Hong Long Ji, Hongyun Fu, Joshua Sill, Xiaoli Zhao, and Ian Pepper
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0301 basic medicine ,Pulmonary and Respiratory Medicine ,Pathology ,medicine.medical_specialty ,Ventilator-Induced Lung Injury ,Clinical Biochemistry ,Cell ,Lung injury ,Alveolar cells ,Mice ,03 medical and health sciences ,In vivo ,Administration, Inhalation ,Animals ,Humans ,Medicine ,Lung ,Molecular Biology ,Cells, Cultured ,Tidal volume ,Original Research ,Wound Healing ,business.industry ,Respiration ,Cell Membrane ,Membrane Proteins ,Cell Biology ,respiratory system ,Respiration, Artificial ,Recombinant Proteins ,Rats ,Pulmonary Alveoli ,030104 developmental biology ,medicine.anatomical_structure ,Breathing ,Carrier Proteins ,business ,Immunostaining - Abstract
Studies showed that TRIM72 is essential for repair of alveolar cell membrane disruptions, and exogenous recombinant human TRIM72 protein (rhT72) demonstrated tissue-mending properties in animal models of tissue injury. Here we examine the mechanisms of rhT72-mediated lung cell protection in vitro and test the efficacy of inhaled rhT72 in reducing tissue pathology in a mouse model of ventilator-induced lung injury. In vitro lung cell injury was induced by glass beads and stretching. Ventilator-induced lung injury was modeled by injurious ventilation at 30 ml/kg tidal volume. Affinity-purified rhT72 or control proteins were added into culture medium or applied through nebulization. Cellular uptake and in vivo distribution of rhT72 were detected by imaging and immunostaining. Exogenous rhT72 maintains membrane integrity of alveolar epithelial cells subjected to glass bead injury in a dose-dependent manner. Inhaled rhT72 decreases the number of fatally injured alveolar cells, and ameliorates tissue-damaging indicators and cell injury markers after injurious ventilation. Using in vitro stretching assays, we reveal that rhT72 improves both cellular resilience to membrane wounding and membrane repair after injury. Image analysis detected rhT72 uptake by rat alveolar epithelial cells, which can be inhibited by a cholesterol-disrupting agent. In addition, inhaled rhT72 distributes to the distal lungs, where it colocalizes with phosphatidylserine detection on nonpermeabilized lung slices to label wounded cells. In conclusion, our study showed that inhaled rhT72 accumulates in injured lungs and protects lung tissue from ventilator injury, the mechanisms of which include improving cell resilience to membrane wounding, localizing to injured membrane, and augmenting membrane repair.
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- 2018
17. Clustered Regularly Interspaced Short Palindromic Repeats/CRISPR-Associated Protein 9 Mediated Knockout Reveals Functions of the yellow-y Gene in Spodoptera litura
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Yi-Lin Yang, Xiao-Long Liu, Wei-Kang Han, Shuang-Lin Dong, Jin Zhang, Yingchuan Peng, Qi Yan, and Long-Ji Ze
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0301 basic medicine ,Malpighian tubule system ,animal structures ,Physiology ,Spodoptera litura ,Biology ,medicine.disease_cause ,Marker gene ,lcsh:Physiology ,yellow-y ,03 medical and health sciences ,0302 clinical medicine ,Physiology (medical) ,medicine ,CRISPR ,pigmentation ,Gene ,CRISPR/Cas9 ,Genetics ,Mutation ,Larva ,lcsh:QP1-981 ,molting ,fungi ,biology.organism_classification ,030104 developmental biology ,Moulting ,030217 neurology & neurosurgery - Abstract
Yellowgenes are thought to be involved in the melanin biosynthetic pathway and play a crucial role in pigmentation reactions in insects. However, little research has been done onyellowgenes in lepidopteran pests. To clarify the function of one of theyellowgenes (yellow-y) inSpodoptera litura, we cloned the full-length ofyellow-y, and investigated its spatial and temporal expression profiles by quantitative real-time PCR (qPCR). It revealed thatyellow-ywas highly expressed in larva of fourth, fifth, and sixth instars, as well as in epidermis (Ep), fat bodies (FB), Malpighian tubes (MT), and midguts (MG) of the larvae; whereas it was expressed in very low levels in different tissues of adults, and was almost undetected in pupa. This expression profile suggests an important role ofyellow-yin larvae, minor role in adults, and no role in pupae. To confirm this, we disruptedyellow-yusing the clustered regularly interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) system, and obtained G0 insects with mutation inyellow-y. The mutation inyellow-yclearly rendered the larvae body, a color yellower than that of wide type insects, and in addition, the mutation resulted in abnormal segmentation and molting for older larvae. The mutation ofyellow-yalso made various adult tissues (antennae, proboscis, legs, and wings) yellowish. However, the mutation had no effect on pigmentation of the pupal cuticle. Taken together, our study clearly demonstrated the role ofyellow-ynot only in the body pigmentation of larvae and adults, and but also in segmentation and molting of larvae, providing new insights into the physiology of larval development, as well as a useful marker gene for genome editing based studies.
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- 2020
18. Knockdown lncRNA DLEU1 Inhibits Gliomas Progression and Promotes Temozolomide Chemosensitivity by Regulating Autophagy
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Qiao-Li Lv, Li-Chong Wang, Dang-Chi Li, Qian-Xia Lin, Xiao-Li Shen, Hai-Yun Liu, Min Li, Yu-Long Ji, Chong-Zhen Qin, and Shu-Hui Chen
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0301 basic medicine ,Cell cycle checkpoint ,Epithelial-Mesenchymal Transition ,Biology ,03 medical and health sciences ,0302 clinical medicine ,Glioma ,medicine ,Temozolomide ,Autophagy ,Gene silencing ,Pharmacology (medical) ,Epithelial–mesenchymal transition ,Original Research ,Pharmacology ,Gene knockdown ,lcsh:RM1-950 ,HOTAIR ,medicine.disease ,030104 developmental biology ,LncRNA DLEU1 ,lcsh:Therapeutics. Pharmacology ,030220 oncology & carcinogenesis ,Cancer research ,medicine.drug - Abstract
Gliomas are the most fatal malignant cerebral tumors. Temozolomide (TMZ), as the primary chemotherapy drug, has been widely used in clinics. However, resistance of TMZ still remains to poor defined. LncRNAs have been reported to play crucial roles in progression of various cancers and resistance of multiple drugs. However, the biological function and underlying mechanisms of most lncRNAs in glioma still remains unclear. Based on the TCGA database, a total of 94 differentially expressed lncRNAs, including 16 up-regulated genes and 78 downregulated genes were identified between gliomas and normal brain tissues. Subsequently, lncRNA DLEU1, HOTAIR, and LOC00132111 were tested to be significantly related to overall survival (OS) between high- and low-expression groups. Additionally, we verified that lncRNA DLEU1 was high expressed in 108 gliomas, compared with 19 normal brain tissues. And high expression of lncRNA DLEU1 predicted a poor prognosis (HR = 1.703, 95%CI: 1.133–2.917, p-value = 0.0159). Moreover, functional assays revealed that knockdown of lncRNA DLEU1 could suppress the proliferation by inducing cell cycle arrest at G1 phase and reducing the S phase by down-regulating the CyclinD1 and p-AKT, as the well as migration and invasion by inhibiting the epithelial–mesenchymal transition (EMT) markers, such as ZEB1, N-cadherin, β-catenin and snail in glioma cells. Furthermore, silencing lncRNA DLEU1 suppressed TMZ-activated autophagy via regulating the expression of P62 and LC3, and promoted sensitivity of glioma cells to TMZ by triggering apoptosis. Conclusively, our study indicated that lncRNA DLEU1 might perform as a prognostic potential target and underlying therapeutic target for sensitivity of glioma to TMZ.
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- 2020
19. Feasibility and mechanism of an amine-looping process for efficient CO2 mineralization using alkaline ashes
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Long Zhang, Long Ji, Kangkang Li, Shuiping Yan, Hai Yu, Xuan Zheng, and Liang Feng
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Diethanolamine ,General Chemical Engineering ,Carbonation ,General Chemistry ,Mineralization (soil science) ,Industrial and Manufacturing Engineering ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,Triethanolamine ,medicine ,Environmental Chemistry ,Amine gas treating ,Phytotoxicity ,Leaching (metallurgy) ,Dissolution ,medicine.drug - Abstract
Amine-looping-based CO2 mineralization is a promising technology for simultaneous CO2 absorption, mineralization, and carbonate crystallization in a single step. This paper performed a detailed investigation of the feasibility and underlying mechanism of the amine-looping process using industrial alkaline solid wastes, including one biomass ash (BA) and two coal-fired fly ashes named FA1 and FA2. The CO2 sequestration capacity and CO2 removal efficiency of selected ashes were investigated in five typical amine solutions, including monoethanolamine (MEA), diethanolamine (DEA), triethanolamine (TEA), 2-amino-2-methy-1-propanol (AMP), and piperazine (PZ). The physicochemical property of ashes before and after carbonation and the dissolution of alkaline minerals in various amine solutions were systematically determined to explore the underlying mechanism involved in the amine-looping process. Results show that greater improvement in CO2 removal efficiencies and CO2 sequestration capacities were obtained by selected ashes in amine solutions compared to the traditional CO2 mineralization in the water-ash-CO2 system. It also revealed that amines played important roles in promoting CO2 mass transfer, enhancing Ca2+ leaching, and producing small-sized CaCO3. The largest CO2 sequestration capacity (102.9 g/kg) was achieved by FA1 in PZ solution which was suggested as the preferred solvent for the amine-looping process. In addition, the environmental risk of carbonated ashes for agricultural application in terms of amine loss and phytotoxicity was evaluated. Results implied that the phytotoxicity of carbonated BA could be neglected when a simple centrifugal wash was used to remove the absorbed amine on the surface of carbonated BA whilst the phytotoxicity of selected ashes can be significantly reduced after carbonation reactions.
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- 2022
20. Causal effect of gallstone disease on the risk of coronary heart disease or acute myocardial infarction: a Mendelian randomization study
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Qingan Fu, Tianzhou Shen, Qingyun Yu, Long Jiang, and Renqiang Yang
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Medicine ,Science - Abstract
Abstract Gallstone disease (GSD) is thought to be associated with the risk of coronary heart disease (CHD) or acute myocardial infarction (AMI), which may be due to abnormal cholesterol metabolism. We used multiple Mendelian randomization (MR) methods based on publicly available genome-wide association study data to assess whether this association is genetically causal and to search for loci driving causality. Pooled data for GSD were obtained from FinnGen Biobank and Biobank Japan, while CHD and AMI were obtained as pooled data from the CARDIoGRAMplusC4D consortium. In this MR study, we found a significant negative causal effect of genetic susceptibility to GSD on AMI in the Finnish population, but no causal effect was found on CHD. This causal effect was not confounded by reverse causality and the same findings were obtained in the Japanese population. Furthermore, the negative causal effect of GSD on AMI risk may be driven by the rs4245791-regulated ABCG5/8 protein. In conclusion, the results of this MR study support a negative causal effect of GSD on AMI and suggest that rs4245791 is the causal driver locus of this effect, which provides new ideas and evidence for the prevention and etiologic study of AMI in patients with GSD.
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- 2023
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21. Unmanipulated haploidentical hematopoietic stem cell transplantation is an excellent option for children and young adult relapsed/refractory Philadelphia chromosome-negative B-cell acute lymphoblastic leukemia after CAR-T-cell therapy
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Guan-Hua Hu, Xiang-Yu Zhao, Ying-Xi Zuo, Ying-Jun Chang, Pan Suo, Jun Wu, Yue-Ping Jia, Ai-Dong Lu, Ying-Chun Li, Yu Wang, Shun-Chang Jiao, Long-Ji Zhang, Jun Kong, Chen-Hua Yan, Lan-Ping Xu, Xiao-Hui Zhang, Kai-Yan Liu, Yi-Fei Cheng, Le-Ping Zhang, and Xiao-Jun Huang
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0301 basic medicine ,Oncology ,Male ,Cancer Research ,medicine.medical_specialty ,Adolescent ,Philadelphia Chromosome Negative ,medicine.medical_treatment ,Graft vs Host Disease ,Disease ,Hematopoietic stem cell transplantation ,Immunotherapy, Adoptive ,03 medical and health sciences ,0302 clinical medicine ,Refractory ,Internal medicine ,Precursor B-Cell Lymphoblastic Leukemia-Lymphoma ,medicine ,Humans ,Cumulative incidence ,Philadelphia Chromosome ,Young adult ,Child ,Retrospective Studies ,Salvage Therapy ,business.industry ,Hematopoietic Stem Cell Transplantation ,Infant ,Hematology ,Prognosis ,Confidence interval ,Chimeric antigen receptor ,Survival Rate ,030104 developmental biology ,Drug Resistance, Neoplasm ,030220 oncology & carcinogenesis ,Child, Preschool ,Transplantation, Haploidentical ,Female ,Neoplasm Recurrence, Local ,business ,Follow-Up Studies - Abstract
Although chimeric antigen receptor T-cell (CAR-T) therapy produces a high complete remission rate among patients with relapsed/refractory B-cell acute lymphoblastic leukemia, relapse remains an urgent issue. It is uncertain whether consolidative haploidentical-allogeneic hematopoietic stem cell transplantation (haplo-HSCT) is suitable for achieving sustainable remission. Therefore, we aimed to assess the efficacy and safety of bridging CAR-T therapy to haplo-HSCT. Fifty-two patients with relapsed/refractory Philadelphia chromosome-negative B-cell acute lymphoblastic leukemia who underwent haplo-HSCT after CAR-T therapy were analyzed. The median time from CAR-T therapy to haplo-HSCT was 61 days. After a median follow-up of 24.6 months, the 1-year probabilities of event-free survival, overall survival, and cumulative incidence of relapse were 80.1% (95% confidence interval (CI), 69.0-90.9), 92.3% (95% CI, 85.0-99.5), and 14.1% (95% CI, 10.7-17.4), respectively, while the corresponding 2-year probabilities were 76.0% (95% CI, 64.2-87.7), 84.3% (95% CI, 74.3-94.3), and 19.7% (95% CI, 15.3-24.0), respectively. No increased risk of 2-year cumulative incidence of graft-versus-host disease, treatment-related mortality, or infection was observed. A pre-HSCT measurable residual disease-positive status was an independent factor associated with poor overall survival (hazard radio: 4.201, 95% CI: 1.034-17.063; P = 0.045). Haplo-HSCT may be a safe and effective treatment strategy to improve event-free survival and overall survival after CAR-T therapy.
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- 2020
22. M6A RNA Methylation Regulator HNRNPC Contributes to Tumorigenesis and Predicts Prognosis in Glioblastoma Multiforme
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Chen Junjun, Kai Yu, Shu-Hui Chen, Chong-Zhen Qin, Li-chong Wang, Dangchi Li, Hai-Yun Liu, Ming-Ming Luo, Xiao-Li Shen, Min Li, Qian-Xia Lin, Qiaoli Lv, Yu-Long Ji, and Huan Yang
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0301 basic medicine ,Cancer Research ,HNRNPC ,RNA methylation ,Regulator ,Biology ,Malignancy ,medicine.disease_cause ,lcsh:RC254-282 ,03 medical and health sciences ,glioblastoma multiforme ,0302 clinical medicine ,Glioma ,medicine ,Original Research ,Proportional hazards model ,m6A ,medicine.disease ,lcsh:Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,tumorigenesis ,030104 developmental biology ,Oncology ,030220 oncology & carcinogenesis ,Cancer research ,Immunohistochemistry ,prognosis ,Carcinogenesis ,hnRNPC - Abstract
Glioblastoma multiforme (GBM) is the most malignant glioma with a high death rate. N6-methyladenosine (m6A) RNA methylation plays an increasingly important role in tumors. The current study aimed to determine the function of the regulators of m6A RNA methylation in GBM. We evaluated the difference, interaction, and correlation of these regulators with TCGA database. HNRNPC, WTAP, YTHDF2 and, YTHDF1 were significantly upregulated in GBM. To explore the expression characteristics of regulators in GBM, we defined two subgroups through consensus cluster. HNRNPC, WTAP, and YTHDF2 were significantly upregulated in the cluster2 which had a good overall survival (OS). To investigate the prognostic value of regulators, we used lasso cox regression algorithm to screen an independent prognostic risk characteristic based on the expression of HNRNPC, ZC3H13, and YTHDF2. The prognostic feature between the low and high-risk groups was significantly different (P < 0.05), which could predict significance of prognosis (area under the curve (AUC) = 0.819). Moreover, we used western blot, RT-PCR, and immunohistochemical staining to verify the expression of HNRNPC was associated with malignancy and development of gliomas. Similarly, the high expression of HNRNPC had a good prognosis. In conclusion, HNRNPC is a vital participant in the malignant progression of GBM and might be valuable for prognosis.
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- 2020
23. Structurally diverse steroids with nitric oxide inhibitory activities from Aglaia lawii leaves
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Xiao-Ling Zheng, Peng Sun, Hua-Bin Hu, Qiang Cai, Kai-Long Ji, Zhi-Yong Yu, Yi-Dian Xiao, Jin-Feng Li, Xiao-Nian Li, Chun-Fen Xiao, Dong-Hua Cao, You-Kai Xu, and Zong-Yi Zhang
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0106 biological sciences ,medicine.drug_class ,Stereochemistry ,Plant Science ,Horticulture ,Ring (chemistry) ,Inhibitory postsynaptic potential ,Nitric Oxide ,01 natural sciences ,Biochemistry ,Anti-inflammatory ,Nitric oxide ,chemistry.chemical_compound ,Ic50 values ,medicine ,Molecular Biology ,Meliaceae ,biology ,Molecular Structure ,010405 organic chemistry ,Chemistry ,Pregnane ,Aglaia lawii ,General Medicine ,biology.organism_classification ,Pregnanes ,0104 chemical sciences ,Plant Leaves ,Steroids ,Aglaia ,010606 plant biology & botany - Abstract
Eleven previously uncharacterized steroids, along with three analogs were isolated from Aglaia lawii leaves. Their structures were definitely characterized by the methods of NMR, MS, IR, ECD and X-ray crystallography study. Among these unreported compounds, 3-epi-dyscusin C, 3-epi-lansisterone E and (Z)-2α-hydroxyaglawone were C-21 pregnane steroids incorporating a highly oxygenated ring A, while others were Δ5-3β-hydroxy-7-ketosteroids bearing different ring D and C-17 aliphatic chains. All isolates were evaluated for nitric oxide (NO) inhibitory activities. 3-Epi-dyscusin C, 3-epi-lansisterone E, (Z)-2α-hydroxyaglawone and 17(20)E-dyscusin B showed significant anti-inflammatory activities with IC50 values of NO inhibition less than 10 μM (in the range from 4.47 ± 0.36 to 7.67 ± 0.46 μM).
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- 2020
24. Plasmin improves blood–gas barrier function in oedematous lungs by cleaving epithelial sodium channels
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Xuefeng Su, Hong Long Ji, Gibran Ali, Hongguang Nie, Runzhen Zhao, Michael A. Matthay, Deepa Bhattarai, Xiaoli Zhao, and Yongchang Chang
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0301 basic medicine ,Epithelial sodium channel ,G protein ,Plasmin ,Proteolysis ,medicine.medical_treatment ,Xenopus ,Lung injury ,03 medical and health sciences ,Mice ,Xenopus laevis ,0302 clinical medicine ,Fibrinolysis ,medicine ,Animals ,Fibrinolysin ,Epithelial Sodium Channels ,Lung ,Pharmacology ,Furin ,biology ,medicine.diagnostic_test ,Chemistry ,respiratory system ,biology.organism_classification ,Research Papers ,Cell biology ,030104 developmental biology ,Oocytes ,030217 neurology & neurosurgery ,Ex vivo ,medicine.drug - Abstract
BACKGROUND AND PURPOSE: Lung oedema in association with suppressed fibrinolysis is a hallmark of lung injury. Here, we have tested whether plasmin cleaves epithelial sodium channels (ENaC) to resolve lung oedema fluid. EXPERIMENTAL APPROACH: Human lungs and airway acid‐instilled mice were used for analysing fluid resolution. In silico prediction, mutagenesis, Xenopus oocytes, immunoblotting, voltage clamp, mass spectrometry, and protein docking were combined for identifying plasmin cleavage sites. KEY RESULTS: Plasmin improved lung fluid resolution in both human lungs ex vivo and injured mice. Plasmin activated αβγENaC channels in oocytes in a time‐dependent manner. Deletion of four consensus proteolysis tracts (αΔ432‐444, γΔ131‐138, γΔ178‐193, and γΔ410‐422) eliminated plasmin‐induced activation significantly. Further, immunoblotting assays identified 7 cleavage sites (K126, R135, K136, R153, K168, R178, K179) for plasmin to trim both furin‐cleaved C‐terminal fragments and full‐length human γENaC proteins. In addition, 9 new sites (R122, R137, R138, K150, K170, R172, R180, K181, K189) in synthesized peptides were found to be cleaved by plasmin. These cleavage sites were located in the finger and the thumb, particularly the GRIP domain of human ENaC 3D model composed of two proteolytic centres for plasmin. Novel uncleaved sites beyond the GRIP domain in both α and γ subunits were identified to interrupt the plasmin cleavage‐induced conformational change in ENaC channel complexes. Additionally, plasmin could regulate ENaC activity via the G protein signal. CONCLUSION AND IMPLICATIONS: Plasmin can cleave ENaC to improve blood‐gas exchange by resolving oedema fluid and could be a potent therapy for oedematous lungs.
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- 2020
25. TRIM72 promotes alveolar epithelial cell membrane repair and ameliorates lung fibrosis
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Dianhua Jiang, Rolf D. Hubmayr, Xiaoli Zhao, Jeremy Herrera, Hong Long Ji, Ian Pepper, Robell Morehouse, Nagaraja N. Nagre, Xiaofei Cong, and Andrew C. Pearson
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0301 basic medicine ,Male ,Mice, 129 Strain ,Idiopathic pulmonary fibrosis ,Membrane repair ,Apoptosis ,Pathogenesis ,Tripartite Motif Proteins ,03 medical and health sciences ,Bleomycin ,Mice ,0302 clinical medicine ,Downregulation and upregulation ,Fibrosis ,Medicine ,Animals ,Humans ,Lung ,lcsh:RC705-779 ,Mice, Knockout ,business.industry ,Regeneration (biology) ,Research ,lcsh:Diseases of the respiratory system ,respiratory system ,medicine.disease ,Recombinant Proteins ,respiratory tract diseases ,030104 developmental biology ,medicine.anatomical_structure ,HEK293 Cells ,Cell culture ,030220 oncology & carcinogenesis ,Alveolar Epithelial Cells ,Cancer research ,Tripartite motif family protein 72 ,Female ,business - Abstract
Background Chronic tissue injury was shown to induce progressive scarring in fibrotic diseases such as idiopathic pulmonary fibrosis (IPF), while an array of repair/regeneration and stress responses come to equilibrium to determine the outcome of injury at the organ level. In the lung, type I alveolar epithelial (ATI) cells constitute the epithelial barrier, while type II alveolar epithelial (ATII) cells play a pivotal role in regenerating the injured distal lungs. It had been demonstrated that eukaryotic cells possess repair machinery that can quickly patch the damaged plasma membrane after injury, and our previous studies discovered the membrane-mending role of Tripartite motif containing 72 (TRIM72) that expresses in a limited number of tissues including the lung. Nevertheless, the role of alveolar epithelial cell (AEC) repair in the pathogenesis of IPF has not been examined yet. Method In this study, we tested the specific roles of TRIM72 in the repair of ATII cells and the development of lung fibrosis. The role of membrane repair was accessed by saponin assay on isolated primary ATII cells and rat ATII cell line. The anti-fibrotic potential of TRIM72 was tested with bleomycin-treated transgenic mice. Results We showed that TRIM72 was upregulated following various injuries and in human IPF lungs. However, TRIM72 expression in ATII cells of the IPF lungs had aberrant subcellular localization. In vitro studies showed that TRIM72 repairs membrane injury of immortalized and primary ATIIs, leading to inhibition of stress-induced p53 activation and reduction in cell apoptosis. In vivo studies demonstrated that TRIM72 protects the integrity of the alveolar epithelial layer and reduces lung fibrosis. Conclusion Our results suggest that TRIM72 protects injured lungs and ameliorates fibrosis through promoting post-injury repair of AECs.
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- 2020
26. Stem cell therapy for COVID-19 and other respiratory diseases: Global trends of clinical trials
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Run Zhen Zhao, Hong Long Ji, and Cong Liu
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0301 basic medicine ,Oncology ,medicine.medical_specialty ,Histology ,medicine.medical_treatment ,Cell therapy ,03 medical and health sciences ,Idiopathic pulmonary fibrosis ,0302 clinical medicine ,Internal medicine ,Genetics ,Medicine ,Progenitor cell ,Molecular Biology ,Genetics (clinical) ,COPD ,Lung ,business.industry ,Mesenchymal stem cell ,Minireviews ,Cell Biology ,Stem-cell therapy ,medicine.disease ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Stem cell ,business - Abstract
Respiratory diseases, including coronavirus disease 2019 and chronic obstructive pulmonary disease (COPD), are leading causes of global fatality. There are no effective and curative treatments, but supportive care only. Cell therapy is a promising therapeutic strategy for refractory and unmanageable pulmonary illnesses, as proved by accumulating preclinical studies. Stem cells consist of totipotent, pluripotent, multipotent, and unipotent cells with the potential to differentiate into cell types requested for repair. Mesenchymal stromal cells, endothelial progenitor cells, peripheral blood stem cells, and lung progenitor cells have been applied to clinical trials. To date, the safety and feasibility of stem cell and extracellular vesicles administration have been confirmed by numerous phase I/II trials in patients with COPD, acute respiratory distress syndrome, bronchial dysplasia, idiopathic pulmonary fibrosis, pulmonary artery hypertension, and silicosis. Five routes and a series of doses have been tested for tolerance and advantages of different regimes. In this review, we systematically summarize the global trends for the cell therapy of common airway and lung diseases registered for clinical trials. The future directions for both new clinical trials and preclinical studies are discussed.
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- 2020
27. Relationships between menstrual status and obesity phenotypes in women : a cross-sectional study in northern China
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Weihua Liu, Dong Li, Fengxue Shi, Haitao Zhang, Huamin Liu, Yanru Chen, Xueyu Chen, Long Ji, Wenran Zhang, Xiaohui Wang, Xiaojun Wang, Xinxia Sui, and Hui Xi
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Adult ,medicine.medical_specialty ,China ,Cross-sectional study ,Endocrinology, Diabetes and Metabolism ,030204 cardiovascular system & hematology ,lcsh:Diseases of the endocrine glands. Clinical endocrinology ,Body Mass Index ,Menstruation ,03 medical and health sciences ,0302 clinical medicine ,Diabetes mellitus ,medicine ,Humans ,Obesity ,Risk factor ,lcsh:RC648-665 ,030219 obstetrics & reproductive medicine ,Obstetrics ,business.industry ,General Medicine ,Odds ratio ,Middle Aged ,medicine.disease ,Prognosis ,Menopause ,Phenotypes ,Cross-Sectional Studies ,Phenotype ,Postmenopausal ,Female ,business ,Body mass index ,Research Article ,Follow-Up Studies - Abstract
Background One of most important concerns of postmenopausal women is obesity. The relationships between menstruation status and obesity phenotypes are unclear. This study aimed to assess the associations between menstrual status and different obesity phenotypes in women. Methods In total, 5373 women aged ≥40 years were recruited from the Jidong and Kailuan communities. Basic information was collected via clinical examination, laboratory testing and standardized questionnaires. The women were stratified into the following three groups: menstrual period, menopausal transition period and postmenopausal period. General obesity was defined as a body mass index (BMI) of ≥28 kg/m2. Central obesity was defined as a waist-to-hip ratio (WHR) of > 0.85. Visceral obesity was defined as the presence of nonalcoholic fatty liver disease (NAFLD) and increased pericardial fat volume (PFV). Results The numbers of women in the menstrual, menopausal transition, and postmenopausal periods were 2807 (52.2%), 675 (12.6%) and 1891 (35.2%), respectively. The adjusted odds ratio (OR) and 95% confidence interval (CI) for central obesity among women in the menopausal transition and postmenopausal periods compared with women in the menstrual period were 0.99 (0.82–1.19) and 1.52 (1.26–1.84), respectively. The OR for NAFLD among postmenopausal women was 1.78 (1.44–2.20). The adjusted β-coefficient (standard error, SE) for PFV among postmenopausal women was 41.25 (7.49). The adjusted OR for general obesity among postmenopausal women was 1.01 (0.77–1.34). Conclusions This study demonstrated that menopause is an independent risk factor for central and visceral obesity but not general obesity.
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- 2020
28. Ion transport mechanisms for smoke inhalation injured airway epithelial barrier
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Jianjun Chang, Hong-Long Ji, Hongguang Nie, Zaixing Chen, and Runzhen Zhao
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0303 health sciences ,Tight junction ,Ussing chamber ,Chemistry ,Ouabain ,3. Good health ,Amiloride ,03 medical and health sciences ,0302 clinical medicine ,030220 oncology & carcinogenesis ,Paracellular transport ,medicine ,Biophysics ,Respiratory epithelium ,Transcellular ,Ion transporter ,030304 developmental biology ,medicine.drug - Abstract
Smoke inhalation injury is the leading cause of death in firefighters and victims. Inhaled hot air and toxic smoke are the predominant hazards to the respiratory epithelium. We aimed to analyze the effects of thermal stress and smoke aldehyde on the permeability of the airway epithelial barrier. Transepithelial resistance (RTE) and short-circuit current (ISC) of mouse tracheal epithelial monolayers were digitized by an Ussing chamber setup. Zonula occludens-1 tight junctions were visualized under confocal microscopy. A cell viability test and fluorescein isothiocyanate-dextran assay were performed. Thermal stress (40°C) decreased RTEin a two-phase manner. Meanwhile, thermal stress increased ISCfollowed by its decline. Na+depletion, amiloride (an inhibitor for epithelial Na+channels [ENaCs]), ouabain (a blocker for Na+/K+-ATPase) and CFTRinh-172 (a blocker of cystic fibrosis transmembrane regulator [CFTR]) altered the responses of RTEand ISCto thermal stress. Steady-state 40°C increased activity of ENaCs, Na+/K+-ATPase, and CFTR. Acrolein, one of the main oxidative unsaturated aldehydes in fire smoke, eliminated RTEand ISC. Na+depletion, amiloride, ouabain, and CFTRinh-172 suppressed acrolein-sensitive ISC, but showed activating effects on acrolein-sensitive RTE. Thermal stress or acrolein disrupted zonula occludens-1 tight junctions, increased fluorescein isothiocyanate-dextran permeability but did not cause cell death or detachment. The synergistic effects of thermal stress and acrolein exacerbated the damage to monolayers. In conclusion, the paracellular pathway mediated by the tight junctions and the transcellular pathway mediated by active and passive ion transport pathways contribute to impairment of the airway epithelial barrier caused by thermal stress and acrolein.Graphical HeadlightsThermal stress and acrolein are two essential determinants for smoke-inhalation injury, impairing airway epithelial barrier.Transcellular ion transport pathways via the ENaC, CFTR, and Na/K-ATPase are interrupted by both thermal stress and acrolein, one of the most potent smoke toxins.Heat and acrolein damage the integrity of the airway epithelium through suppressing and relocating the tight junctions.
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- 2020
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29. Plasmin improves oedematous blood-gas barrier by cleaving epithelial sodium channels
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Michael A. Matthay, Deepa Bhattarai, Hong-Long Ji, Yongchang Chang, Gibran Ali, Hong-Guang Nie, Xiaoli Zhao, Runzhen Zhao, and Xuefeng Su
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Epithelial sodium channel ,0303 health sciences ,medicine.diagnostic_test ,biology ,G protein ,Chemistry ,Plasmin ,Proteolysis ,medicine.medical_treatment ,030302 biochemistry & molecular biology ,Xenopus ,Lung injury ,Cleavage (embryo) ,biology.organism_classification ,Cell biology ,03 medical and health sciences ,Fibrinolysis ,medicine ,030304 developmental biology ,medicine.drug - Abstract
Background and PurposeLung oedema in association with suppressed fibrinolysis is a hallmark of lung injury. We aimed to test whether plasmin cleaves epithelial sodium channels (ENaC) to resolve lung oedema fluid.Experimental ApproachesHuman lungs and airway acid-instilled mice were used for analysing fluid resolution. In silico prediction, mutagenesis, Xenopus oocytes, immunoblotting, voltage clamp, mass spectrometry, protein docking, and alveolar fluid clearance were combined for identifying plasmin specific cleavage sites and benefits.Key ResultsPlasmin led to a marked increment in lung fluid resolution in both human lungs ex vivo and injured mice. Plasmin specifically activated αβγENaC channels in oocytes in a time-dependent manner. Deletion of four consensus proteolysis tracts (αΔ432-444, γΔ131-138, γΔ178-193, and γΔ410-422) eliminated plasmin-induced activation significantly. Further, immunoblotting assays identified 7 cleavage sites (K126, R135, K136, R153, K168, R178, K179) for plasmin to trim both furin-cleaved C-terminal fragments and full-length human γENaC proteins. In addition to confirming the 7 cleavage sites, 9 new sites (R122, R137, R138, K150, K170, R172, R180, K181, K189) in synthesized peptides were found to be cleaved by plasmin with mass spectrometry. These cleavage sites were located in the finger and the thumb, particularly the GRIP domain of human ENaC 3D model composed of two proteolytic centres for plasmin. Novel uncleaved sites beyond the GRIP domain in both α and γ subunits were identified to interrupt the plasmin cleavage-induced conformational change in ENaC channel complexes. Additionally, plasmin could regulate ENaC activity via the G protein signal.Conclusion and ImplicationsWe demonstrate that plasmin could cleave ENaC to benefit the blood-gas exchange by resolving oedema fluid as a potent fibrinolytic therapy for oedematous pulmonary diseases.Bullet point summaryWhat is already knowSerine proteases proteolytically cleave epithelial sodium channels, including plasmin and uPA acutely.Activity of epithelial sodium channels is increased post proteolysis.What this study addsPlasmin cleaves up to 16 sites composed of two proteolytic centres in both full-length and furin-cleaved human γ subunit of epithelial sodium channels in hours.Non-proteolytic sites in both α and γ subunits interrupt the plasmin cleavage-induced channel gating.Intratracheally instilled plasmin facilitates alveolar fluid clearance in normal human and injured mouse lungs.Clinical significanceActivation of human lung epithelial sodium channels by plasmin may benefit lung oedema resolution as a novel therapy for ARDS.
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- 2020
30. Seroprevalence and Epidemiological Characteristics of Immunoglobulin M and G Antibodies Against SARS-CoV-2 in Asymptomatic People in Wuhan, China
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Sha Xu, Hong-Long Ji, Tao Li, Renrong Sun, Ruijie Ling, Jiayu He, Jixian Zhang, Yihan Yu, and Huanqiang Wang
- Subjects
medicine.medical_specialty ,education.field_of_study ,biology ,business.industry ,Public health ,Population ,Retrospective cohort study ,Asymptomatic ,Serology ,Immunoglobulin M ,Internal medicine ,Epidemiology ,biology.protein ,Medicine ,Seroprevalence ,medicine.symptom ,business ,education - Abstract
Background: The World Health Organization (WHO) recommends monitoring changes in seroprevalence of immunoglobulin M (IgM) and immunoglobulin G (IgG) antibodies against severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) over time from the beginning of an epidemic to predict and plan accordingly for public health responses. At present, few domestic and foreign reports exist on the diagnosis of COVID-19 patients through serological tests. To detect and assess asymptomatic infections of COVID-19 among people in Wuhan, the epicenter of the COVID-19 pandemic in China, and provide a basis for planning an adequate public health response, we analyzed the records in a Wuhan general hospital of medical examinations mandatory for 16- to 64-year-old asymptomatic people to resume their job-related work. This retrospective study estimated the seroprevalence of IgM and IgG and compared the epidemiological characteristics of asymptomatic SARS-CoV-2- infected workers. Methods: Medical examination data were collected from the Wuhan General Hospital between March 26 and April 28, 2020. Serological tests for the presence of antibodies (IgM and IgG) against SARS-CoV-2 used a colloidal gold method. Nucleic acid sequence of viruses were detected with a fluorescent PCR method. Demographical and radiological data were collected. Statistical analyses were conducted using SPSS 20.0 software. Findings: Between March 26 and April 28, 2020, a total of 18,391 asymptomatic back-to-work participants were enrolled. Among them, 89 had positivity for IgM (0·48%, 95% confidence interval (CI): 0·38–0·58%); 620 cases had IgG positivity (3·37%, 95% CI: 3·11–3·64%), and 650 cases had either IgG positivity or IgM positivity (3·53%, 95% CI: 3·26–3·80%). After standardizing for the genders and ages in the population of Wuhan, the overall standardized seroprevalence of IgG was 3·33% (95% CI: 3·07–3·59%) and the standardized seroprevalence of IgG was 3·01% (95% CI: 2·69–3·33%) among males and 3·66% (95 % CI: 3·23–4·09%) among females. The standardized seroprevalence of IgG was higher in women than in men with a significant difference (x 2 = 2060·3, p < 0·01). By a detection method adjustment, the seroprevalence of IgG was 1·57% (95% CI: 1·39–1·75%) in all medical records, of which males were 1·96% (95% CI: 1·64–2·28%), and females were 1·19% (95% CI: 0·99–1·39%). The assayadjusted seroprevalence of IgG was higher in women than in men, and the difference was significant (x 2 = 5871·0, p < 0·01). The differences were significant for seroprevalence of IgG among people who went back to work in different categories of work place (x 2 = 198·44, p < 0·01). The differences in seroprevalence for IgG positivity or IgM positivity among people who went back to work in different urban and rural areas was also significant (x 2 = 45·110, p < 0·01). Calculated as IgG and/or IgM antibody positivity, the number of new infections was reduced by 64·8% from March 26 to April 28, 2020. Based on the census population aged 16–64 years in Wuhan in 2017, we estimated that 172,340 (95% CI: 157,568–187,112) asymptomatic people aged 16–64 years were infected with SARS-CoV-2 in Wuhan between March 25 and April 28 2020. This estimate was 3·4-times higher than the officially reported 50,333 infections on April 28. Interpretation: The seropositivity rate in Wuhan indicated that RT-PCR-confirmed patients only represented a small part of the total number of cases. Seropositivity progressively decreased in the Wuhan population from March 26 to April 28, 2020, comparable to Japan and Denmark, but well below the level reported in New York, Iran, Italy, and Germany. The prevalence of asymptomatic infection was higher in women than in men among people who went back to work in Wuhan. The low seroprevalence suggests that most of the population remains susceptible to COVID19. Funding: Emergency Management Project of the National Natural Science Foundation of China (81842035) ; Advisory Research Project of the Chinese Academy of Engineering in 2019 (2019-XZ-70) Declaration of Interests: All authors declare no conflicts of interest. Ethics Approval Statement: This study was approved by the Ethics Committee of the Hubei Provincial Hospital of Integrated Chinese & Western Medicine. This was a retrospective and observational study and informed consent was obtained.
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- 2020
31. Bamboo shoot fiber improves insulin sensitivity in high-fat diet-fed mice
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MM Dahab, You-Kai Xu, Juan Guo, Ping Zhang, Baiting Fu, Xiu-Fen Li, and Kai-Long Ji
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0301 basic medicine ,medicine.medical_specialty ,p38 mitogen-activated protein kinases ,medicine.medical_treatment ,Medicine (miscellaneous) ,PGC-1α ,03 medical and health sciences ,Internal medicine ,medicine ,TX341-641 ,Fiber ,Protein kinase B ,030109 nutrition & dietetics ,Nutrition and Dietetics ,biology ,Chemistry ,Nutrition. Foods and food supply ,Insulin ,AMPK ,Glucose tolerance ,Insulin sensitivity ,Dietary fiber ,Bamboo shoot ,Insulin signaling ,Insulin receptor ,030104 developmental biology ,Endocrinology ,Bamboo shoot fiber ,biology.protein ,Phosphorylation ,Food Science - Abstract
The aim of this study was to evaluate the role of bamboo shoot fiber (BSF) in regulating insulin sensitivity in mice. Two BSFs were obtained from D. hamiltonii and D. latiforus. C57BL/6 mice were fed a low-fat diet or a high-fat diet with 10% fiber as cellulose (HFC), or two BSFs (HFDH or HFDL) for 13 weeks. Compared with the HFC group, HFDH and HFDL groups exhibited considerably less body weight gain, lower levels of fasting glucose and insulin, and lower values of glucose areas under the curve during glucose and insulin tolerance tests. BSF groups also had lower glucoses stimulated insulin concentrations. In addition, more phosphorylation of Akt in insulin target tissues was associated with higher protein expression levels of PGC-1α and phosphorylated AMPK and p38 in BSF fed mice. These results indicated that BSF improved insulin sensitivity in high-fat diet-fed mice through enhancing insulin signaling and activating PGC-1α.
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- 2018
32. Association between high‐sensitivity C‐reactive protein, lipoprotein‐associated phospholipase A2 and carotid atherosclerosis: A cross‐sectional study
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Dong Li, Haitao Hu, Huamin Liu, Qinghua Cui, Youxin Wang, Jianxin Chen, Kai Zhu, Qing Liu, Long Ji, Yan Yao, Bin Geng, Yong Zhou, and Jichun Yang
- Subjects
Adult ,Carotid Artery Diseases ,Male ,medicine.medical_specialty ,Genotype ,Cross-sectional study ,carotid atherosclerosis ,030204 cardiovascular system & hematology ,Logistic regression ,Polymorphism, Single Nucleotide ,03 medical and health sciences ,0302 clinical medicine ,high‐sensitivity C‐reactive protein ,Risk Factors ,Internal medicine ,Medicine ,Humans ,Genetic Predisposition to Disease ,Genetic Association Studies ,Aged ,combination ,biology ,business.industry ,lipoprotein‐associated phospholipase A2 ,Lipoprotein-associated phospholipase A2 ,C-reactive protein ,Cell Biology ,Odds ratio ,Original Articles ,Middle Aged ,medicine.disease ,Confidence interval ,Stenosis ,C-Reactive Protein ,Cross-Sectional Studies ,Logistic Models ,1-Alkyl-2-acetylglycerophosphocholine Esterase ,biology.protein ,Cardiology ,Molecular Medicine ,lipids (amino acids, peptides, and proteins) ,Original Article ,Female ,business ,Nephelometry ,030217 neurology & neurosurgery ,Biomarkers - Abstract
High‐sensitivity C‐reactive protein (hs‐CRP) and lipoprotein‐associated phospholipase A2 (Lp‐PLA2) have been reported to be independent predictors of atherosclerosis. However, whether the combination of these two markers can improve the prediction of atherosclerosis is unknown. This study aimed to evaluate the association between combining hs‐CRP and Lp‐PLA2 and predicting carotid atherosclerosis. A total of 1982 participants aged ≥40 years were included in this study. Hs‐CRP and Lp‐PLA2 were measured by a high‐sensitivity nephelometry assay and quantitative sandwich enzyme‐linked immunosorbent assay, respectively. Ultrasonography was performed on the bilateral carotid arteries to evaluate stenosis and plaques. Multivariable logistic regression models were used to analyse the association between the combination of the hs‐CRP and Lp‐PLA2 levels and carotid plaques and stenosis. A total of 1579 (79.7%) and 181 (9.1%) subjects had carotid plaques and carotid stenosis, respectively. The group with high hs‐CRP and Lp‐PLA2 levels had the highest prevalence of carotid plaques (90.6%) and stenosis (20.8%). A significant association was found between high hs‐CRP and Lp‐PLA2 levels and carotid stenosis (adjusted odds ratio [OR]: 2.39; 95% confidence interval [CI]: 1.13‐5.09), but this combination was not associated with carotid plaques (OR: 2.62, 95% CI: 0.93‐7.38). The results suggested that the combination of hs‐CRP and Lp‐PLA2 were better predictors than either protein alone with regard to carotid atherosclerosis.
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- 2018
33. Gene editing as a promising approach for respiratory diseases
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Yana Ma, Hong Long Ji, Chonghua Ren, Yichun Bai, Yang Liu, Zhenlei Su, and Runzhen Zhao
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Gene Editing ,Lung Diseases ,0301 basic medicine ,Transcription activator-like effector nuclease ,Lung ,business.industry ,Cas9 ,Gene mutation ,Bioinformatics ,Zinc Finger Nucleases ,03 medical and health sciences ,030104 developmental biology ,medicine.anatomical_structure ,Respiratory failure ,Genome editing ,Transcription Activator-Like Effector Nucleases ,Mutation ,Genetics ,Humans ,Medicine ,CRISPR ,CRISPR-Cas Systems ,Respiratory system ,business ,Genetics (clinical) - Abstract
Respiratory diseases, which are leading causes of mortality and morbidity in the world, are dysfunctions of the nasopharynx, the trachea, the bronchus, the lung and the pleural cavity. Symptoms of chronic respiratory diseases, such as cough, sneezing and difficulty breathing, may seriously affect the productivity, sleep quality and physical and mental well-being of patients, and patients with acute respiratory diseases may have difficulty breathing, anoxia and even life-threatening respiratory failure. Respiratory diseases are generally heterogeneous, with multifaceted causes including smoking, ageing, air pollution, infection and gene mutations. Clinically, a single pulmonary disease can exhibit more than one phenotype or coexist with multiple organ disorders. To correct abnormal function or repair injured respiratory tissues, one of the most promising techniques is to correct mutated genes by gene editing, as some gene mutations have been clearly demonstrated to be associated with genetic or heterogeneous respiratory diseases. Zinc finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN) and clustered regulatory interspaced short palindromic repeats/CRISPR-associated protein 9 (CRISPR/Cas9) systems are three innovative gene editing technologies developed recently. In this short review, we have summarised the structure and operating principles of the ZFNs, TALENs and CRISPR/Cas9 systems and their preclinical and clinical applications in respiratory diseases.
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- 2018
34. Well-differentiated adenocarcinoma may be misdiagnosed as gastritis cystica profunda
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Jing-Yuan Xia and Xiao-Long Ji
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Pathology ,medicine.medical_specialty ,business.industry ,Gastritis cystica profunda ,medicine ,Adenocarcinoma ,medicine.disease ,business ,Well differentiated - Published
- 2017
35. Fibrinolytic or anti-plasmin (nafamostat) therapy for COVID-19: A timing challenge for clinicians
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Brant M. Wagener, Runzhen Zhao, Hong-Long Ji, and Timothy J. Ness
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Pulmonary and Respiratory Medicine ,2019-20 coronavirus outbreak ,Coronavirus disease 2019 (COVID-19) ,business.industry ,Plasmin ,Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) ,Biochemistry (medical) ,COVID-19 ,Guanidines ,Article ,Benzamidines ,COVID-19 Drug Treatment ,Fibrinolytic therapy ,anti-protease ,Nafamostat ,Immunology ,Humans ,Medicine ,Pharmacology (medical) ,Fibrinolysin ,business ,plasmin ,medicine.drug ,Anti proteases - Published
- 2021
36. Structurally diverse limonoids and bio-active evaluation from Trichilia connaroides
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Xiao-Nian Li, Chun-Fen Xiao, Jian-Neng Yao, Qiang Cai, Hua-Bin Hu, Kai-Long Ji, Dong-Hua Cao, Zhi-Yong Yu, Peng Sun, and You-Kai Xu
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Limonins ,China ,Trichilia connaroides ,Stereochemistry ,Phytochemicals ,Anti-Inflammatory Agents ,Limonoid ,Mice ,Cell Line, Tumor ,Drug Discovery ,medicine ,Ic50 values ,Animals ,Humans ,Meliaceae ,Pharmacology ,Molecular Structure ,biology ,Chemistry ,General Medicine ,biology.organism_classification ,Plant Leaves ,RAW 264.7 Cells ,Human cancer ,medicine.drug - Abstract
Four new limonoids, named as trichiconlide G (1), 2-hydroxyltrijugin F (2), 23-oxo-21-hydroxyltrijugin F (3), 21-oxo-23-hydroxyltrijugin F (4), along with sixteen known analogues (5–20) were isolated from the leaves and twigs of Trichilia connaroides. Their structures and absolute configurations were determined by spectroscopic analyses, X-ray diffraction analysis, and TD-DFT-ECD calculations. Trichiconlide G (1) is one rare naturally occurring 1,2-seco phragmalin-type limonoid bearing a C-7/28 δ-lactone ring. Additionally, 2-hydroxyltrijugin F (2), 23-oxo-21-hydroxyltrijugin F (3), and 21-oxo-23-hydroxyltrijugin F (4) are three naturally occurring limonoids with a rare C-16/8 δ-lactone ring. All isolates were evaluated for their cytotoxic and anti-inflammatory activities. None of compounds exhibited cytotoxicity against five human cancer cell lines A-549, HepG2, 5-8F, Siha, and SCC-4 at the concentration of 40 μM. Compounds 16 and 17 showed moderate anti-inflammatory activity with IC50 values of 28.45 ± 2.51 and 22.66 ± 2.01 μM, respectively.
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- 2021
37. Activation of UBEC2 by transcription factor MYBL2 affects DNA damage and promotes gastric cancer progression and cisplatin resistance
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Long Jiegen, Zhu Bin, Tian Tao, Ren Linfei, Tao Yong, Zhu Haitao, Li Dengwei, and Xu Yonghong
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mybl2 ,ube2c ,gastric cancer ,dna damage ,cisplatin resistance ,Medicine - Abstract
Ubiquitin-conjugating enzyme E2 C (UBE2C) plays a carcinogenic role in gastric cancer (GC); yet, its role in cisplatin (DDP) resistance in GC is enigmatic. This study sought to probe into the impact of UBE2C on DDP resistance in GC and its concrete molecular mechanism in GC progression. Bioinformatics analysis was used to analyze differentially expressed mRNAs and predict upstream regulatory molecules in GC. Real-time quantitative reverse transcriptase polymerase chain reaction and western blot were used to detect the expression of UBE2C and MYB proto-oncogene like 2 (MYBL2). Dual luciferase and chromatin immunoprecipitation (ChIP) assays were used to verify the binding relationship. Cell counting kit-8 was used to detect cell viability and calculate IC50 values. Flow cytometry was used to detect the cell cycle. Comet assay was used to detect DNA damage. Western blot was used to detect the expression of DNA loss-related proteins (γ-H2AX, ATM/p-ATM). The knockdown of highly expressed UBE2C in GC cell lines could reduce cell viability, induce G2/M arrest, induce apoptosis, and promote DNA damage and DDP sensitivity. Bioinformatics analysis predicted that the substantially upregulated MYBL2 was an upstream transcription factor in UBE2C. The binding relationship between the UBE2C promoter region and MYBL2 was verified by dual luciferase and ChIP. Overexpression of UBE2C in the rescue experiment was found to reverse the inhibited GC progression and promoted DDP sensitivity brought by the knockdown of MYBL2. In conclusion, the MYBL2/UBE2C regulatory axis may be a potential way to overcome DDP resistance in GC.
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- 2023
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38. Insights into dual functions of amino acid salts as CO2 carriers and CaCO3 regulators for integrated CO2 absorption and mineralisation
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Long Zhang, Shuiping Yan, Qingyao He, Long Ji, Liang Feng, and Xuan Zheng
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Diethanolamine ,Process Chemistry and Technology ,Carbonation ,Potassium ,chemistry.chemical_element ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Solvent ,Piperazine ,chemistry.chemical_compound ,chemistry ,Triethanolamine ,Desorption ,medicine ,Chemical Engineering (miscellaneous) ,Chelation ,0210 nano-technology ,Waste Management and Disposal ,Nuclear chemistry ,medicine.drug - Abstract
Integrated CO2 absorption and mineralisation (IAM) is a promising technology for rapidly CO2 absorption and sequestration. However, the traditional IAM using alkanolamines as CO2 solvents and solid wastes as carbonation feedstocks still suffers from the utilization difficulty of low value products and environmental risks of amine degradation. In this study, the green solvent, amino acid salts (AAS), including potassium l -argininate (ArgK), potassium glycinate, potassium sarcosinate, potassium l -alaninate, potassium β-alaninate, were selected for IAM using rejected brine as the carbonation feedstock. Results shows that compared to amines (monoethanolamine, diethanolamine, N-methyldiethanolamine, triethanolamine, piperazine, 2-amino-2-methy-1-propanol), the selected AASs in IAM were not only more effective CO2 carriers that passed CO2 to calcium ions to form CaCO3, but also better CaCO3 growth regulators to tune morphology properties of products. CO2 desorption efficiencies in CO2-loaded AASs (nearly 100 %) were much higher than those of in CO2-loaded amines especially than in MEA (38 %). ArgK stood out in regulating the morphology of crystal with smallest particle size. Then ArgK was selected for optimization experiments at various conditions (temperature, Ca2+/CO2 ratio, reactant concentration and chelating agent) to further investigate effects of operating conditions on CaCO3 properties and to provide some basic information for the polymorph and morphology regulation of CaCO3. Ethylene diamine tetraacetic acid (EDTA) as a chelating agent was found a notable influence on controlling the polymorph of CaCO3, where the amorphous CaCO3 and new structures of calcite appeared. However, the effect of EDTA on CO2 absorption step was not clear and required further investigation.
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- 2021
39. Novel mechanisms for crotonaldehyde-induced lung edema
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Runzhen Zhao, Yapeng Hou, Yong Cui, Yue Li, Yan Ding, Hongguang Nie, Zhiyu Zhou, Jianjun Chang, and Hong Long Ji
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0301 basic medicine ,Epithelial sodium channel ,Lung injury ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Western blot ,Dichlorofluorescein ,crotonaldehyde ,Extracellular ,Medicine ,lung injury ,Crotonaldehyde ,reactive oxygen species ,medicine.diagnostic_test ,business.industry ,Kinase ,alveolar fluid clearance ,respiratory system ,Molecular biology ,030104 developmental biology ,Oncology ,chemistry ,Immunology ,business ,030217 neurology & neurosurgery ,Intracellular ,Research Paper ,epithelial sodium channels - Abstract
// Yue Li 1, * , Jianjun Chang 1, * , Yong Cui 2 , Runzhen Zhao 3 , Yan Ding 1 , Yapeng Hou 1 , Zhiyu Zhou 1 , Hong-Long Ji 3, 4 and Hongguang Nie 1 1 Institute of Metabolic Disease Research and Drug Development, China Medical University, Shenyang 110122, Liaoning, China 2 Department of Anesthesiology, First Affiliated Hospital of China Medical University, Shenyang 110001, Liaoning, China 3 Department of Cellular and Molecular Biology, University of Texas Health Science Center at Tyler, Tyler, Texas 75708, USA 4 Texas Lung Injury Institute, University of Texas Health Northeast, Tyler, Texas 75708, USA * These authors have contributed equally to this work Correspondence to: Hongguang Nie, email: hgnie@cmu.edu.cn Hong-Long Ji, email: james.ji@uthct.edu Keywords: epithelial sodium channels, crotonaldehyde, lung injury, alveolar fluid clearance, reactive oxygen species Received: March 17, 2017 Accepted: April 25, 2017 Published: May 12, 2017 ABSTRACT Background: Crotonaldehyde is a highly noxious α,β-unsaturated aldehyde in cigarette smoke that causes edematous acute lung injury. Objective: To understand how crotonaldehyde impairs lung function, we examined its effects on human epithelial sodium channels (ENaC), which are major contributors to alveolar fluid clearance. Methods: We studied alveolar fluid clearance in C57 mice and ENaC activity was examined in H441 cells. Expression of α- and γ-ENaC was measured at protein and mRNA levels by western blot and real-time PCR, respectively. Intracellular ROS levels were detected by the dichlorofluorescein assay. Heterologous αβγ-ENaC activity was observed in an oocyte model. Results: Our results showed that crotonaldehyde reduced transalveolar fluid clearance in mice. Furthermore, ENaC activity in H441 cells was inhibited by crotonaldehyde dose-dependently. Expression of α- and γ-subunits of ENaC was decreased at the protein and mRNA level in H441 cells exposed to crotonaldehyde, which was probably mediated by the increase in phosphorylated extracellular signal-regulated protein kinases 1 and 2. ROS levels increased time-dependently in cells exposed to crotonaldehyde. Heterologous αβγ-ENaC activity was rapidly eliminated by crotonaldehyde. Conclusion: Our findings suggest that crotonaldehyde causes edematous acute lung injury by eliminating ENaC activity at least partly via facilitating the phosphorylation of extracellular signal-regulated protein kinases 1 and 2 signal molecules. Long-term exposure may decrease the expression of ENaC subunits and damage the cell membrane integrity, as well as increase the levels of cellular ROS products.
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- 2017
40. Serious adverse events of cell therapy for respiratory diseases: a systematic review and meta-analysis
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Zhenlei Su, Hong Long Ji, Jing Wu, and Runzhen Zhao
- Subjects
0301 basic medicine ,Spirometry ,medicine.medical_specialty ,ARDS ,Respiratory Tract Diseases ,Cell- and Tissue-Based Therapy ,Review ,Lung injury ,03 medical and health sciences ,Idiopathic pulmonary fibrosis ,0302 clinical medicine ,systematic review ,Internal medicine ,Odds Ratio ,medicine ,Humans ,030212 general & internal medicine ,Adverse effect ,Intensive care medicine ,Clinical Trials as Topic ,COPD ,Lung ,medicine.diagnostic_test ,respiratory diseases ,business.industry ,Mesenchymal Stem Cells ,medicine.disease ,meta-analysis ,Treatment Outcome ,030104 developmental biology ,medicine.anatomical_structure ,Oncology ,Bronchopulmonary dysplasia ,serious adverse events ,cell therapy ,business - Abstract
// Runzhen Zhao 1,* , Zhenlei Su 2,* , Jing Wu 2 and Hong-Long Ji 1 1 Texas Lung Injury Institute, University of Texas Health Northeast, Tyler, Texas, USA 2 Institute of Lung and Molecular Therapy, Xinxiang Medical University, Xinxiang, Henan, China * These authors have contributed equally to this work Correspondence to: Hong-Long Ji, email: // Keywords : serious adverse events, cell therapy, respiratory diseases, systematic review, meta-analysis Received : January 17, 2017 Accepted : February 08, 2017 Published : February 16, 2017 Abstract Background: Cell therapy holds the most promising for acute and chronic deleterious respiratory diseases. However, the safety and tolerance for lung disorders are controversy. Methods: We undertook a systematic review and meta-analyses of all 23 clinical studies of cell therapy. The outcomes were odds ratio (OR), risk difference (RD), Peto OR, relative risk, and mean difference of serious adverse events. Results: 342 systemic infusions and 57 bronchial instillations (204 recipients) of cells were analyzed for acute respiratory distress syndrome (ARDS), bronchopulmonary dysplasia, pulmonary arterial hypertension, silicosis, sarcoidosis, extensively drug-resistant tuberculosis, chronic obstructive pulmonary diseases (COPD), and idiopathic pulmonary fibrosis. The frequency of death in adults from any causes was 71 and 177 per 1,000 for cell therapy and controls, respectively, with an OR of 0.31 (95% CI: 0.03, 3.76) and RD of -0.22 (95% CI: -0.53, 0.09). No significant difference was found for ARDS and COPD. The frequency of deaths and non-fatal serious adverse events of 17 open studies were similar to those of randomized controlled trials. Moreover, serious adverse events of allogenic cells were greater than autologous preparations, as shown by frequency, OR and RD. Conclusions: We conclude that either infusion or instillation of mesenchymal stem stromal or progenitor cells are well tolerated without serious adverse events causally related to cell treatment. Cell therapy has not been associated with significant changes in spirometry, immune function, cardiovascular activity, and the quality of life.
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- 2017
41. ENaCs as Both Effectors and Regulators of MiRNAs in Lung Epithelial Development and Regeneration
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Michael A. Matthay, Runzhen Zhao, Xiaoli Zhao, Yan Ding, Hong Guang Nie, and Hong Long Ji
- Subjects
0301 basic medicine ,Epithelial sodium channel ,Physiology ,Cross talk ,Biology ,lcsh:Physiology ,Epithelium ,Article ,lcsh:Biochemistry ,03 medical and health sciences ,Paracrine signalling ,microRNA ,medicine ,Animals ,Humans ,Regeneration ,lcsh:QD415-436 ,Epithelial reparation ,Amiloride-sensitive sodium channels ,Progenitor cell ,Epithelial Sodium Channels ,Stem/progenitor cells ,Non-coding RNA ,Lung ,Transepithelial sodium transport ,Kidney ,lcsh:QP1-981 ,urogenital system ,Stem Cells ,Mesenchymal stem cell ,respiratory system ,3. Good health ,Cell biology ,MicroRNAs ,030104 developmental biology ,medicine.anatomical_structure ,Stem cell ,hormones, hormone substitutes, and hormone antagonists ,Re-epithelialization - Abstract
Epithelial sodium channels (ENaC) play an important role in re-absorbing excessive luminal fluid by building up an osmotic Na+ gradient across the tight epithelium in the airway, the lung, the kidney, and the colon. The ENaC is a major pathway for retention of salt in kidney too. MicroRNAs (miRs), a group of non-coding RNAs that regulate gene expression at the post-transcriptional level, have emerged as a novel class of regulators for ENaC. Given the ENaC pathway is crucial for maintaining fluid homeostasis in the lung and the kidney and other cavities, we summarized the cross-talk between ENaC and miRs and recapitulated the underlying regulatory factors, including aldosterone, transforming growth factor-β1, and vascular endothelial growth factor-A in the lung and other epithelial tissues/organs. We have compared the profiling of miRs between normal and injured mice and human lungs, which showed a significant alteration in numerous miRs in mouse models of LPS and ventilator induced ARDS. In addition, we reiterated the potential regulation of the ENaC by miRs in stem/ progenitor cell-based re-epithelialization, and identified a promising pharmaceutic target of ENaC for removing edema fluid in ARDS by mesenchymal stem cells-released paracrine. In conclusion, it seems that the interactions between miRs and scnn1s/ENaCs are critical for lung development, epithelial cell turnover in adult lungs, and re-epithelialization for repair.
- Published
- 2017
42. Ion transport mechanisms for smoke inhalation-injured airway epithelial barrier
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Jianjun Chang, Runzhen Zhao, Hongguang Nie, Hong-Long Ji, and Zaixing Chen
- Subjects
0301 basic medicine ,Male ,Hot Temperature ,Health, Toxicology and Mutagenesis ,Cystic Fibrosis Transmembrane Conductance Regulator ,Bronchi ,Toxicology ,Ouabain ,Permeability ,Article ,Tight Junctions ,03 medical and health sciences ,0302 clinical medicine ,Smoke ,medicine ,Electric Impedance ,Animals ,Humans ,Transcellular ,Acrolein ,Epithelial Sodium Channels ,Ion transporter ,Cells, Cultured ,Inhalation Exposure ,Ion Transport ,Ussing chamber ,Tight junction ,Chemistry ,Membrane Transport Proteins ,Epithelial Cells ,Cell Biology ,Smoke Inhalation Injury ,3. Good health ,Amiloride ,Mice, Inbred C57BL ,Trachea ,030104 developmental biology ,030220 oncology & carcinogenesis ,Paracellular transport ,Biophysics ,Zonula Occludens-1 Protein ,Respiratory epithelium ,Female ,Sodium-Potassium-Exchanging ATPase ,medicine.drug - Abstract
Smoke inhalation injury is the leading cause of death in firefighters and victims. Inhaled hot air and toxic smoke are the predominant hazards to the respiratory epithelium. We aimed to analyze the effects of thermal stress and smoke aldehyde on the permeability of the airway epithelial barrier. Transepithelial resistance (R(TE)) and short-circuit current (I(SC)) of mouse tracheal epithelial monolayers were digitized by an Ussing chamber setup. Zonula occludens-1 tight junctions were visualized under confocal microscopy. A cell viability test and fluorescein isothiocyanate-dextran assay were performed. Thermal stress (40°C) decreased R(TE) in a two-phase manner. Meanwhile, thermal stress increased I(SC) followed by its decline. Na(+) depletion, amiloride (an inhibitor for epithelial Na(+) channels [ENaCs]), ouabain (a blocker for Na(+)/K(+)-ATPase) and CFTRinh-172 (a blocker of cystic fibrosis transmembrane regulator [CFTR]) altered the responses of R(TE) and I(SC) to thermal stress. Steady-state 40°C increased activity of ENaCs, Na(+)/K(+)-ATPase, and CFTR. Acrolein, one of the main oxidative unsaturated aldehydes in fire smoke, eliminated R(TE) and I(SC). Na(+) depletion, amiloride, ouabain, and CFTRinh-172 suppressed acrolein-sensitive I(SC), but showed activating effects on acrolein-sensitive R(TE). Thermal stress or acrolein disrupted zonula occludens-1 tight junctions, increased fluorescein isothiocyanate-dextran permeability but did not cause cell death or detachment. The synergistic effects of thermal stress and acrolein exacerbated the damage to monolayers. In conclusion, the paracellular pathway mediated by the tight junctions and the transcellular pathway mediated by active and passive ion transport pathways contribute to impairment of the airway epithelial barrier caused by thermal stress and acrolein.
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- 2019
43. Maternal Body Mass Index and Risk of Congenital Heart Defects in Infants: A Dose-Response Meta-Analysis
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Yuejin Li, Huamin Liu, Long Ji, Weili Yang, Qianqian Zhang, Xuezhen Liu, Xia Feng, Dong Li, and Guoyong Ding
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Heart Defects, Congenital ,medicine.medical_specialty ,lcsh:Medicine ,Subgroup analysis ,Review Article ,030204 cardiovascular system & hematology ,Overweight ,General Biochemistry, Genetics and Molecular Biology ,Body Mass Index ,03 medical and health sciences ,0302 clinical medicine ,Pregnancy ,Risk Factors ,Medicine ,Humans ,Mass index ,030212 general & internal medicine ,Obesity ,General Immunology and Microbiology ,business.industry ,Obstetrics ,lcsh:R ,Infant, Newborn ,Infant ,General Medicine ,Publication bias ,Pregnancy Complications ,Relative risk ,Meta-analysis ,Cohort ,Female ,medicine.symptom ,business ,Body mass index - Abstract
Objective. The exact shape of the dose-response relationship between maternal body mass index (BMI) and the risk of congenital heart defects (CHDs) in infants has not been clearly defined yet. This study aims to further clarify the relationship between maternal obesity and the risk of CHDs in infants by an overall and dose-response meta-analysis. Methods. PubMed, Embase, and Web of Science databases were searched to identify all related studies. The studies were limited to human cohort or case-control studies in English language. Random-effect models and dose-response meta-analysis were used to synthesize the results. Heterogeneity, subgroup analysis, sensitivity analysis, and publication bias were also assessed. Results. Nineteen studies with 2,416,546 participants were included in our meta-analysis. Compared with the mothers with normal weight, the pooled relative risks (RRs) of infants with CHDs were 1.08 (95% CI=1.03-1.13) in overweight and 1.23 (95% CI=1.17-1.29) in obese mothers. According to the findings from the linear meta-analysis, we observed an increased risk of infants with CHDs (RR=1.07, 95% CI=1.06-1.08) for each 5 kg/m2 increase in maternal BMI. A nonlinear relationship between maternal BMI and risk of infants with CHDs was also found (p=0.012). Conclusion. The results from our meta-analysis indicate that increased maternal BMI is related to increased risk of CHDs in infants.
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- 2019
44. Preclinical and clinical studies of smoke-inhalation-induced acute lung injury: update on both pathogenesis and innovative therapy
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Bingxin Guo, Hong Long Ji, Cong Liu, Jiaxing Dong, Song Wang, Yana Ma, Runzhen Zhao, and Yichun Bai
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Pulmonary and Respiratory Medicine ,medicine.medical_specialty ,Smoke inhalation ,Review ,030204 cardiovascular system & hematology ,Lung injury ,pathophysiology of lung injury ,Pathogenesis ,03 medical and health sciences ,0302 clinical medicine ,Animal model ,medicine ,Animals ,Humans ,Pharmacology (medical) ,Intensive care medicine ,intervention ,lcsh:RC705-779 ,Respiratory illness ,business.industry ,Therapies, Investigational ,030208 emergency & critical care medicine ,lcsh:Diseases of the respiratory system ,Airway obstruction ,Smoke Inhalation Injury ,medicine.disease ,3. Good health ,Airway Obstruction ,Disease Models, Animal ,Oxidative Stress ,Innovative Therapies ,acute lung injury ,Thermal damage ,smoke inhalation ,business - Abstract
Smoke-inhalation-induced acute lung injury (SI-ALI) is a leading cause of morbidity and mortality in victims of fire tragedies. SI-ALI contributes to an estimated 30% of burn-caused patient deaths, and recently, more attention has been paid to the specific interventions for this devastating respiratory illness. In the last decade, much progress has been made in the understanding of SI-ALI patho-mechanisms and in the development of new therapeutic strategies in both preclinical and clinical studies. This article reviews the recent progress in the treatment of SI-ALI, based on pathophysiology, thermal damage, airway obstruction, the nuclear-factor kappa-B signaling pathway, and oxidative stress. Preclinical therapeutic strategies include use of mesenchymal stem cells, hydrogen sulfide, peroxynitrite decomposition catalysts, and proton-pump inhibitors. Clinical interventions include high-frequency percussive ventilation, perfluorohexane, inhaled anticoagulants, and nebulized epinephrine. The animal model, dose, clinical application, and pharmacology of these medications are summarized. Future directions and further needs for developing innovative therapies are discussed. The reviews of this paper are available via the supplementary material section.
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- 2019
45. Plasmin Cleaves Human Epithelial Sodium Channels to Resolve Lung Edema
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Gibran Ali, Deepa Bhattarai, Runzhen Zhao, Hongguang Nie, and Hong Long Ji
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Epithelial sodium channel ,Chemistry ,Plasmin ,medicine ,LUNG EDEMA ,Molecular biology ,medicine.drug - Published
- 2019
46. Upregulation of the WNK4 Signaling Pathway Inhibits Epithelial Sodium Channels of Mouse Tracheal Epithelial Cells After Influenza A Infection
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Yapeng Hou, Yong Cui, Zhiyu Zhou, Hongfei Liu, Honglei Zhang, Yan Ding, Hongguang Nie, and Hong-Long Ji
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0301 basic medicine ,Epithelial sodium channel ,ENaC ,influenza virus ,Virus ,03 medical and health sciences ,0302 clinical medicine ,WNK4 ,Western blot ,Downregulation and upregulation ,airway surface liquid ,medicine ,Pharmacology (medical) ,Original Research ,Pharmacology ,Gene knockdown ,Ussing chamber ,medicine.diagnostic_test ,urogenital system ,Chemistry ,lcsh:RM1-950 ,respiratory system ,Fluid transport ,Cell biology ,lcsh:Therapeutics. Pharmacology ,030104 developmental biology ,030220 oncology & carcinogenesis ,MTECs ,Signal transduction - Abstract
Influenza virus has a significant impact on the respiratory system. The mechanism of how influenza virus impairs the fluid transport in airway is not fully understood. We examined its effects on epithelial sodium channels (ENaC), which are very important for water and salt transport in the respiratory system. We focused on the impacts of influenza virus on ENaC activity in mouse tracheal epithelial cells (MTECs) and applied Ussing chamber apparatus for recording the short-circuit currents in primary cultured MTECs. Expressions of α and γ-ENaC were measured at the protein and mRNA levels by western blot and quantitative real-time polymerase chain reaction, respectively. Roles of the with-no-lysine-kinase-4 (WNK4) pathway were considered in participating influenza virus-involved ENaC regulation by using siRNA to knockdown WNK4 and the physical properties of airway surface liquid (ASL) were detected by confocal microscopy. Our results showed that influenza virus reduced ENaC activity, and the expressions of α and γ-ENaC were decreased at the protein and mRNA levels, respectively. WNK4 expression increased time-dependently at the protein level after influenza virus infection, while knockdown of WNK4 rescued the impact of influenza virus on ENaC and ASL height increased obviously after MTECs were treated with influenza virus. Taken together, these results suggest that influenza virus causes the changes of biophysical profile in the airway by altering the ENaC activity at least partly via facilitating the expression of WNK4.
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- 2019
47. Metabolic systems approaches update molecular insights of clinical phenotypes and cardiovascular risk in patients with homozygous familial hypercholesterolemia
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Zhiyong Du, Fan Li, Long Jiang, Linyi Li, Yunhui Du, Huahui Yu, Yan Luo, Yu Wang, Haili Sun, Chaowei Hu, Jianping Li, Ya Yang, Xiaolu Jiao, Luya Wang, and Yanwen Qin
- Subjects
Homozygous familial hypercholesterolemia ,Metabolomics ,Inflammation ,Corneal arcus ,Xanthomas ,Aortic stenosis ,Medicine - Abstract
Abstract Background Homozygous familial hypercholesterolemia (HoFH) is an orphan metabolic disease characterized by extremely elevated low-density lipoprotein cholesterol (LDL-C), xanthomas, aortic stenosis, and premature atherosclerotic cardiovascular disease (ASCVD). In addition to LDL-C, studies in experimental models and small clinical populations have suggested that other types of metabolic molecules might also be risk factors responsible for cardiovascular complications in HoFH, but definitive evidence from large-scale human studies is still lacking. Herein, we aimed to comprehensively characterize the metabolic features and risk factors of human HoFH by using metabolic systems strategies. Methods Two independent multi-center cohorts with a total of 868 individuals were included in the cross-sectional study. First, comprehensive serum metabolome/lipidome-wide analyses were employed to identify the metabolomic patterns for differentiating HoFH patients (n = 184) from heterozygous FH (HeFH, n = 376) and non-FH (n = 100) subjects in the discovery cohort. Then, the metabolomic patterns were verified in the validation cohort with 48 HoFH patients, 110 HeFH patients, and 50 non-FH individuals. Subsequently, correlation/regression analyses were performed to investigate the associations of clinical/metabolic alterations with typical phenotypes of HoFH. In the prospective study, a total of 84 HoFH patients with available follow-up were enrolled from the discovery cohort. Targeted metabolomics, deep proteomics, and random forest approaches were performed to investigate the ASCVD-associated biomarkers in HoFH patients. Results Beyond LDL-C, various bioactive metabolites in multiple pathways were discovered and validated for differentiating HoFH from HoFH and non-FH. Our results demonstrated that the inflammation and oxidative stress-related metabolites in the pathways of arachidonic acid and lipoprotein(a) metabolism were independently associated with the prevalence of corneal arcus, xanthomas, and supravalvular/valvular aortic stenosis in HoFH patients. Our results also identified a small marker panel consisting of high-density lipoprotein cholesterol, lipoprotein(a), apolipoprotein A1, and eight proinflammatory and proatherogenic metabolites in the pathways of arachidonic acid, phospholipid, carnitine, and sphingolipid metabolism that exhibited significant performances on predicting first ASCVD events in HoFH patients. Conclusions Our findings demonstrate that human HoFH is associated with a variety of metabolic abnormalities and is more complex than previously known. Furthermore, this study provides additional metabolic alterations that hold promise as residual risk factors in HoFH population.
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- 2023
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48. CTLA4-Ig protects tacrolimus-induced oxidative stress via inhibiting the AKT/FOXO3 signaling pathway in rats
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Long Jin, Nan Shen, Xinyu Wen, Weidong Wang, Sun Woo Lim, and Chul Woo Yang
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renal injury ,tacrolimus ,ctla4-ig ,akt/foxo3 signaling pathway ,Medicine - Abstract
Background/Aims Although the conversion from tacrolimus (TAC) to cytotoxic T-lymphocyte-associated antigen 4-immunoglobulin (CTLA4-Ig) is effective in reducing TAC-induced nephrotoxicity, it remains unclear whether CTLA4-Ig has a direct effect on TAC-induced renal injury. In this study, we evaluated the effects of CTLA4-Ig on TAC-induced renal injury in terms of oxidative stress. Methods in vitro study was performed to assess the effect of CTLA4-Ig on TAC-induced cell death, reactive oxygen species (ROS), apoptosis, and the protein kinase B (AKT)/forkhead transcription factor (FOXO) 3 pathway in human kidney 2 cells. In the in vivo study, the effect of CTLA4-Ig on TAC-induced renal injury was evaluated using renal function, histopathology, markers of oxidative stress (8-hydroxy-2’-deoxyguanosine) and metabolites (4-hydroxy-2-hexenal, catalase, glutathione S-transferase, and glutathione reductase), and activation of the AKT/FOXO3 pathway with insulin-like growth factor 1 (IGF-1). Results CTLA4-Ig significantly decreased cell death, ROS, and apoptosis caused by TAC. TAC treatment increased apoptotic cell death and apoptosis-related proteins (increased Bcl-2-associated X protein and caspase-3 and decreased Bcl-2), but it was reversed by CTLA4-Ig treatment. The activation of p-AKT and p-FOXO3 by TAC decreased with CTLA4-Ig treatment. TAC-induced renal dysfunction and oxidative marker levels were significantly improved by CTLA4-Ig in vivo. Concomitant IGF-1 treatment abolished the effects of CTLA4-Ig. Conclusions CTLA4-Ig has a direct protective effect on TAC-induced renal injury via the inhibition of AKT/FOXO3 pathway.
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- 2023
- Full Text
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49. Diverse roles of miR-335 in development and progression of cancers
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Long-ji Luo, Dan-Dan Wang, Fan Yang, Jing Wang, and Jinhai Tang
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0301 basic medicine ,General Medicine ,Biology ,Bioinformatics ,medicine.disease_cause ,medicine.disease ,Biomarker (cell) ,Metastasis ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,In vivo ,Tumor progression ,030220 oncology & carcinogenesis ,microRNA ,Gene expression ,medicine ,Carcinogenesis ,Gene - Abstract
MicroRNAs (miRNAs), a series of small noncoding RNAs that regulate gene expression at the post-transcriptional/translational level, are pivotal in cell differentiation, biological development, occurrence, and development of diseases, especially in cancers. Early studies have shown that miRNA-335 (miR-335) is widely dysregulated in human cancers and play critical roles in tumorigenesis and tumor progression. In this review, we aim to summarize the regulation of miR-335 expression mechanisms in cancers. We focus on the target genes regulated by miR-335 and its downstream signaling pathways involved in the biological effects of tumor growth, invasion, and metastasis both in vitro and in vivo, and analyze the relationships between miR-335 expression and the clinical characteristics of tumors as well as its effects on prognosis. The collected evidences support the potential use of miR-335 in prognosis and diagnosis as well as the therapeutic prospects of miR-335 in cancers.
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- 2016
50. miR-222 induces Adriamycin resistance in breast cancer through PTEN/Akt/p27kip1 pathway
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Long-ji Luo, Jianhua Zhao, Shanliang Zhong, Sujin Yang, Jinhai Tang, Xiaohui Zhang, Dan-Dan Wang, Xiu Chen, and Hongyu Shen
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0301 basic medicine ,medicine.medical_specialty ,Flow cytometry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Breast cancer ,Downregulation and upregulation ,Internal medicine ,medicine ,PTEN ,LY294002 ,skin and connective tissue diseases ,Protein kinase B ,biology ,medicine.diagnostic_test ,business.industry ,Cancer ,General Medicine ,medicine.disease ,030104 developmental biology ,Endocrinology ,chemistry ,Apoptosis ,030220 oncology & carcinogenesis ,biology.protein ,Cancer research ,business - Abstract
The high resistant rate of Adriamycin (Adr) is associated with a poor prognosis of breast cancer in women worldwide. Since miR-222 might contribute to chemoresistance in many cancer types, in this study, we aimed to investigate its efficacy in breast cancer through PTEN/Akt/p27 kip1 pathway. Firstly, in vivo, we verified that miR-222 was upregulated in chemoresistant tissues after surgery compared with the paired preneoadjuvant samples of 21 breast cancer patients. Then, human breast cancer Adr-resistant cell line (MCF-7/Adr) was constructed to validate the pathway from the parental sensitive cell line (MCF-7/S). MCF-7/Adr and MCF-7/S were transfected with miR-222 mimics, miR-222 inhibitors, or their negative controls, respectively. The results showed that inhibition of miR-222 in MCF-7/Adr significantly increased the expressions of PTEN and p27 kip1 and decreased phospho-Akt (p-Akt) both in mRNA and protein levels (p < 0.05) by using quantitative real-time PCR (qRT-PCR) and western blot. MTT and flow cytometry suggested that lower expressed miR-222 enhanced apoptosis and decreased the IC50 of MCF-7/Adr cells. Additionally, immunofluorescence demonstrated that the subcellular location of p27 kip1 was dislocated resulting from the alteration of miR-222. Conversely, in MCF-7/S transfected with miR-222 mimics, upregulation of miR-222 is associated with decreasing PTEN and p27 kip1 and increasing Akt accompanied by less apoptosis and higher IC50. Importantly, Adr resistance induced by miR-222 overexpression through PTEN/Akt/p27 was completely blocked by LY294002, an Akt inhibitor. Taken together, these data firstly elucidated that miR-222 could reduce the sensitivity of breast cancer cells to Adr through PTEN/Akt/p27 kip1 signaling pathway, which provided a potential target to increase the sensitivity to Adr in breast cancer treatment and further improved the prognosis of breast cancer patients.
- Published
- 2016
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