1. Resveratrol and insulin association reduced alveolar bone loss and produced an antioxidant effect in diabetic rats
- Author
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Howard C. Tenenbaum, Fernanda Vieira Ribeiro, Andréia Manetta Molez, Márcio Zaffalon Casati, Fabiano Ribeiro Cirano, Mônica Grazieli Corrêa, and Suzana Peres Pimentel
- Subjects
Male ,0301 basic medicine ,medicine.medical_specialty ,medicine.medical_treatment ,Alveolar Bone Loss ,medicine.disease_cause ,Antioxidants ,Diabetes Mellitus, Experimental ,Bone remodeling ,Superoxide dismutase ,03 medical and health sciences ,0302 clinical medicine ,Osteoprotegerin ,Internal medicine ,Diabetes mellitus ,Animals ,Insulin ,Medicine ,Rats, Wistar ,NADPH oxidase ,biology ,business.industry ,030206 dentistry ,medicine.disease ,Streptozotocin ,Rats ,030104 developmental biology ,Endocrinology ,Resveratrol ,biology.protein ,Periodontics ,business ,Oxidative stress ,medicine.drug - Abstract
BACKGROUND The present investigation studied the effects of systemic administration of resveratrol (RSV) on the development of experimental periodontitis (EP) and on the release of markers of inflammation, bone metabolism, and oxidative stress in diabetic rats. METHODS Seventy-five male rats were divided into five groups: DM+PLAC: Diabetes Mellitus + placebo solution; DM+INS: DM + insulin therapy; DM+RSV: DM + RSV; DM+RSV+INS: DM + RSV and insulin; NDM: non-diabetic. Streptozotocin was used to induce DM and EP was induced by the placement of a ligature at the fist mandibular and the second maxillary molars. Euthanasia occurred 30 days after the initiation of the study and mandible specimens were subjected for morphometric analysis of bone level. Gingival tissues from mandibular molars were collected for quantification of inflammatory and oxidative stress markers by multiplex assay system and ELISA assay, respectively. Maxillary gingival tissues were processed for real-time polymerase chain reaction (real-time PCR) assessment of markers of bone metabolism and oxidative stress. RESULTS Morphometric analysis revealed greater bone loss in DM+PLAC and DM+INS in comparison to the other treatments (P
- Published
- 2020