Back to Search
Start Over
Chronic Stress May Modulate Periodontal Disease: A Study in Rats
- Source :
- Journal of Periodontology. 79:697-704
- Publication Year :
- 2008
- Publisher :
- Wiley, 2008.
-
Abstract
- The present study aimed to evaluate whether chronic stress (CS) affects ligature-induced periodontal disease and to investigate the impact of CS on the mRNA levels of interleukin (IL)-1beta, -1 receptor antagonist, -6, and -10, interferon-gamma (IFN-gamma), receptor activator of nuclear factor-kappa B ligand (RANKL), and osteoprotegerin in the gingival tissues of rats.Sixty male Wistar rats were assigned randomly to three groups: G1 (control; non-ligated sites), G2 (periodontal disease), and G3 (periodontal disease associated with restraint stress for 12 hours/day for the entire study). After 30 days, all animals were sacrificed by decapitation. Blood samples were taken, and the concentrations of corticosterone and catecholamines were measured as biomarkers of CS. Marginal tissues around ligated and non-ligated teeth were harvested, and gene expression was assessed by quantitative polymerase chain reaction. Moreover, the area of bone loss (ABL) was determined histometrically.Data analysis showed that CS increased serum levels of stress biomarkers (P0.05), ligature placement resulted in a significant ABL compared to non-ligated sites, CS significantly increased the amount of ABL in inflamed sites (P0.001), and CS significantly increased mRNA levels of proinflammatory (IL-1beta and -6 and IFN-gamma) and anti-inflammatory (IL-10) cytokines and proresorptive factor (RANKL) in ligated sites (P0.05).CS significantly increased bone loss resulting from ligature-induced periodontitis by a local increase in proinflammatory and proresorptive factors.
- Subjects :
- Male
medicine.medical_specialty
medicine.drug_class
Interleukin-1beta
Alveolar Bone Loss
Gingiva
Interferon-gamma
Random Allocation
chemistry.chemical_compound
Catecholamines
Osteoprotegerin
Stress, Physiological
Corticosterone
Internal medicine
Animals
Medicine
Chronic stress
RNA, Messenger
Rats, Wistar
Periodontitis
Receptor
biology
Interleukin-6
business.industry
RANK Ligand
Receptors, Interleukin-1
Interleukin
Receptor antagonist
Interleukin-10
Rats
Interleukin 1 Receptor Antagonist Protein
Real-time polymerase chain reaction
Endocrinology
chemistry
RANKL
Chronic Disease
biology.protein
Periodontics
Inflammation Mediators
business
Biomarkers
Subjects
Details
- ISSN :
- 19433670 and 00223492
- Volume :
- 79
- Database :
- OpenAIRE
- Journal :
- Journal of Periodontology
- Accession number :
- edsair.doi.dedup.....e104bea490fd55aaca0aef31b20465a5