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Resveratrol attenuates oxidative stress during experimental periodontitis in rats exposed to cigarette smoke inhalation
- Source :
- Journal of periodontal research. 54(3)
- Publication Year :
- 2018
-
Abstract
- OBJECTIVES This study aimed at investigating the effect of the systemic administration of resveratrol (RESV) on oxidative stress during experimental periodontitis in rats subjected to cigarette smoke inhalation. MATERIAL AND METHODS Experimental periodontitis (EP) was induced in 26 male Wistar rats by the insertion of a ligature around one of the first mandibular and maxillary molars. The animals were assigned randomly to the following groups: cigarette smoke inhalation (CSI; 3 times/d, 8 minutes/d) + resveratrol (10 mg/Kg), that is, SMK + RESV (n = 13) and cigarette smoke inhalation + placebo, that is, SMK + PLAC (n = 13). The substances were administered daily for 30 days (19 days prior and 11 days following EP induction), and then, the animals were euthanized. The maxillary specimens were processed for morphometric analysis of bone loss, and the tissue surrounding the first maxillary molars was collected for mRNA quantification of Sirtuin 1 (SIRT1) by real-time PCR. The gingival tissues surrounding the mandibular first molars were collected for quantification of superoxide dismutase 1 (SOD1) and nicotinamide adenine dinucleotide phosphatase oxidase (NADPH) using an ELISA assay. RESULTS Reduced bone loss was demonstrated in animals in the SMK + RESV group as compared to those in the SMK + PLAC (P
- Subjects :
- 0301 basic medicine
Molar
Male
medicine.medical_specialty
Gingiva
Enzyme-Linked Immunosorbent Assay
Mandible
Resveratrol
medicine.disease_cause
Polymerase Chain Reaction
Antioxidants
Cigarette Smoking
Superoxide dismutase
03 medical and health sciences
chemistry.chemical_compound
0302 clinical medicine
Superoxide Dismutase-1
Sirtuin 1
Internal medicine
medicine
Animals
RNA, Messenger
Rats, Wistar
Periodontitis
NADPH oxidase
Inhalation
biology
business.industry
Anti-Inflammatory Agents, Non-Steroidal
030206 dentistry
medicine.disease
Oxidative Stress
030104 developmental biology
Endocrinology
chemistry
biology.protein
Periodontics
business
Oxidative stress
NADP
Subjects
Details
- ISSN :
- 16000765
- Volume :
- 54
- Issue :
- 3
- Database :
- OpenAIRE
- Journal :
- Journal of periodontal research
- Accession number :
- edsair.doi.dedup.....3c99875a5ed6d2276912de1ad7cdde4c