1. Insulin‐like growth factor 2 mRNA‐binding protein 3 enhanced melanoma migration through regulation of AKT1 and RELA expression.
- Author
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Sheen, Yi‐Shuan, Syu, Yan‐Jie, Chang, Yu‐Chuan, Hsieh, Ping‐Han, Liao, Yi‐Hua, Lin, Ming‐Hsien, Chen, Chien‐Yu, Chu, Chia‐Yu, and Chu, Chia‐Ying
- Subjects
SOMATOMEDIN A ,MICROPHTHALMIA-associated transcription factor ,GENE expression ,MELANOMA - Abstract
IMP‐3 expression is a poor prognostic factor of melanomas and it promotes melanoma cell migration and invasion by a pathway modulating HMGA2 mRNA expression. We tried to identify other putative targets of IMP‐3. We identified putative IMP‐3‐binding RNAs, including AKT1, MAPK3, RB1 and RELA, by RNA immunoprecipitation coupled with next‐generation sequencing. IMP‐3 overexpression increased AKT and RELA levels in MeWo cells. siRNAs against AKT1 and RELA inhibited MeWo/Full‐length IMP‐3 cell migration. IMP‐3 knockdown of A2058 cells decreased AKT1 and RELA expression and lowered migration ability. Co‐transfection of A2058 cells with AKT1‐ or RELA‐expressing plasmids with IMP‐3 siRNA restored the inhibitory effects of IMP‐3 knockdown on migration. HMGA2 did not influence AKT1 and RELA expression in melanoma cells. Human melanoma samples with high IMP‐3 levels also showed high HMGA2, AKT1 and RELA expression. Our results show that IMP‐3 enhances melanoma cell migration through the regulation of the AKT1 and RELA axis. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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