Back to Search
Start Over
Identification mouse patatin-like phospholipase domain containing protein 1 as a skin-specific and membrane-associated protein
- Source :
- Gene. 591(2)
- Publication Year :
- 2016
-
Abstract
- Patatin-like phospholipase domain containing protein 1 (PNPLA1) mutations have been identified to be associated with autosomal recessive congenital ichthyosis (ARCI) in recent years. However, its molecular characters have not been achieved until now. In the current study, the full length coding cDNA sequence of mouse PNPLA1 (mPNPLA1) was identified firstly. There were several putative transmembrane domains (TMDs) in mPNPLA1 by bioinformation analysis. mPNPLA1 was further found to be expressed exclusively in the membrane fraction in mammalian cells. However, it did not colocalized with the endoplasmic reticulum (ER) or lipid droplets (LDs). Moreover, the mRNA levels of mPNPLA1 was detected to be highly expressed in the skin, while very weak or even less in other mouse tissues by quantitative PCR. In addition, based on experiments with inhibitors and inducer of protein degradation pathways, mPNPLA1 was demonstrated to be degraded by macroautophagy, but not by the proteasome. These results indicated PNPLA1 was a skin-specific and membrane-associated protein for the first time, suggesting that it may mainly play a role in the skin.
- Subjects :
- 0301 basic medicine
Protein domain
Gene Expression
Protein degradation
Biology
Endoplasmic Reticulum
03 medical and health sciences
Mice
Protein Domains
Congenital ichthyosis
Chlorocebus aethiops
Genetics
Animals
Humans
Amino Acid Sequence
Peptide sequence
Skin
Endoplasmic reticulum
Membrane Proteins
General Medicine
Lipase
Lipid Droplets
Sequence Analysis, DNA
Molecular biology
Transmembrane domain
030104 developmental biology
Patatin-like phospholipase
Membrane protein
Phospholipases
COS Cells
Mutation
Sequence Alignment
Subjects
Details
- ISSN :
- 18790038
- Volume :
- 591
- Issue :
- 2
- Database :
- OpenAIRE
- Journal :
- Gene
- Accession number :
- edsair.doi.dedup.....6ed574c91df45108270628176a2e22fd