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Assessing the impact of polysomy-17 on HER2 status and the correlations of HER2 status with prognostic variables (ER, PR, p53, Ki-67) in epithelial ovarian cancer: a tissue microarray study using immunohistochemistry and fluorescent in situ hybridization
- Source :
- International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists. 30(4)
- Publication Year :
- 2011
-
Abstract
- Although HER2 overexpression and Her2 amplification have been noted in breast and a variety of human cancers, we report here for the first time the impact of polysomy-17 on HER2 status and the correlations between HER2 status and other prognostic factors in patients with epithelial ovarian cancers (EOC).We analyzed HER2, estrogen receptor (ER), progesterone receptor (PR), p53, and Ki-67 protein overexpressions by immunohistochemistry (IHC) and determined Her2 gene amplification by fluorescence in situ hybridization (FISH) in 27 tissue microarray samples from EOC patients.We achieved 100% positive concordance (3/3) and 100% negative concordance (19/19) between HER2 testing by IHC and FISH. Both the total Her2 gene copies and FISH scores increased significantly in a stepwise order through the negative, equivocal, and positive HER2 IHC result categories in all 27 cases (P=0.001, P=0.001), and still increased significantly in 18 nonpolysomy-17 cases (P=0.007 and 0.013) after the exclusion of 9 polysomy-17 cases. HER2 protein expression is inversely correlated with both ER (P=0.002) and PR expressions (P=0.046). Her2 gene amplification is inversely correlated with ER expression (P=0.007) but not with PR expression (P=0.106).This study showed extremely high positive and negative concordances between Her2 FISH and HER2 IHC assays. Polysomy-17 is insufficient for causing a significant impact on the relationship between HER2 testing by IHC and FISH in EOC. ER and PR expressions were inversely correlated with HER2 protein expression. In addition, ER but not PR expression is inversely correlated with Her2 gene amplification.
- Subjects :
- Pathology
medicine.medical_specialty
Receptor, ErbB-2
Estrogen receptor
Gene Expression
In situ hybridization
Biology
Pathology and Forensic Medicine
Progesterone receptor
medicine
Humans
skin and connective tissue diseases
neoplasms
In Situ Hybridization, Fluorescence
Ovarian Neoplasms
Polysomy
Tissue microarray
medicine.diagnostic_test
Obstetrics and Gynecology
medicine.disease
Aneuploidy
Microarray Analysis
Prognosis
Immunohistochemistry
Ki-67 Antigen
Receptors, Estrogen
Ki-67
biology.protein
Female
Tumor Suppressor Protein p53
Receptors, Progesterone
Fluorescence in situ hybridization
Chromosomes, Human, Pair 17
Subjects
Details
- ISSN :
- 15387151
- Volume :
- 30
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- International journal of gynecological pathology : official journal of the International Society of Gynecological Pathologists
- Accession number :
- edsair.doi.dedup.....49908da6bf8a53957efc0e7cd9e8b61a