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Long intergenic non‑protein coding RNA 00460 predicts a poor prognosis and promotes tumorigenesis of human osteosarcoma
- Source :
- Molecular Medicine Reports
- Publication Year :
- 2019
- Publisher :
- Spandidos Publications, 2019.
-
Abstract
- Osteosarcoma (OS) is the most common type of primary malignant bone tumor, which has a high incidence rate in children and adolescents. This research aims to reveal the role of long intergenic non-protein coding RNA 00460 (LINC00460) in OS by the loss-of-function experiment. LINC00460 is involved in the development of multiple types of tumor, but the role of LINC00460 in OS is unclear. To discover more effective molecular targets for the treatment of OS, the association between LINC00460 and OS prognosis was analyzed using the Gene Expression Profiling Interactive Analysis database. Additionally, small interfering RNA was used to knockdown LINC00460 gene expression in vitro to verify its biological effects on the viability, invasive and migratory potential of OS cells. LINC00460 knockdown significantly reduced the viability of OS cells and initiated cell cycle arrest within the G0/G1 phase through the decreased expression of cyclin D1 and CDK4/CDK6. In addition, LINC00460 knockdown promoted apoptosis of OS cells, and inhibited the migratory and invasive abilities of OS cells through the inhibition of the epithelial-mesenchymal transition pathway. In conclusion, the present study reported that LINC00460 may predict OS prognosis, and may serve an important role in mediating the viability, invasive and migratory potential of OS cells. Based on these findings, LINC00460 demonstrated promising potential as a future therapeutic target for OS treatment.
- Subjects :
- 0301 basic medicine
Cancer Research
Small interfering RNA
Epithelial-Mesenchymal Transition
Carcinogenesis
Cell Survival
Apoptosis
Biology
Resting Phase, Cell Cycle
Biochemistry
03 medical and health sciences
0302 clinical medicine
Cyclin D1
Cell Movement
Cell Line, Tumor
Gene expression
Genetics
medicine
Gene Knockdown Techniques
Humans
Neoplasm Invasiveness
RNA, Small Interfering
Molecular Biology
long intergenic non-protein coding RNA 00460
Osteosarcoma
Gene knockdown
G1 Phase
Cyclin-Dependent Kinase 4
Articles
Cell Cycle Checkpoints
Cyclin-Dependent Kinase 6
Cell cycle
Prognosis
medicine.disease
Gene expression profiling
030104 developmental biology
Matrix Metalloproteinase 9
Oncology
cell cycle arrest
030220 oncology & carcinogenesis
Cancer research
Molecular Medicine
RNA, Long Noncoding
Subjects
Details
- ISSN :
- 17913004 and 17912997
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....cf4a583cdd7bfedfef4b82af8311fd69