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Gene expression analysis: Regulation of key genes associated with mycophenolate mofetil treatment of symptomatic carotid artery stenosis
- Source :
- Molecular Medicine Reports
- Publication Year :
- 2017
- Publisher :
- D.A. Spandidos, 2017.
-
Abstract
- The present study analyzed gene expression arrays to identify differentially-expressed genes (DEGs) between mycophenolate mofetil (MMF)‑treated and placebo‑treated patients with symptomatic carotid artery stenosis (SCAS). In addition, the key genes involved in the pharmacology of MMF treatment in patients with SCAS were identified. The gene expression dataset was obtained from a Gene Expression Omnibus database, which included 9 MMF‑treated and 11 placebo‑treated samples. The DEGs were identified between MMF and placebo groups using R software. Furthermore, a protein‑protein interaction (PPI) network of the identified DEGS was constructed. The Database for Annotation, Visualization and Integrated Discovery was used to perform Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses of the 19 most significant DEGs. A total of 210 DEGs between the MMF and placebo groups were screened and their PPI was constructed. GO function analysis revealed that the 19 DEGs were predominantly involved in the tyrosine phosphorylation of signal transducer and activator of transcription‑5 protein, which is closely associated with the activation of T cells. The KEGG pathway analysis suggested that the main metabolic pathways of the 19 DEGs were associated with the pharmacological functioning of MMF in activated T cells. In conclusion, the present study identified numerous key DEGs associated with SCAS, and the results suggested that v‑kit Hardy‑Zuckerman 4 feline sarcoma viral oncogene homolog and apelin may serve important roles in the MMF treatment of SCAS.
- Subjects :
- 0301 basic medicine
Cancer Research
genetic structures
030204 cardiovascular system & hematology
Biology
Bioinformatics
carotid artery stenosis
Biochemistry
03 medical and health sciences
0302 clinical medicine
Gene expression
Databases, Genetic
Genetics
Cluster Analysis
Humans
Carotid Stenosis
Protein Interaction Maps
KEGG
genes
Molecular Biology
Gene
Principal Component Analysis
Oncogene
Activator (genetics)
Computational Biology
Articles
Cell cycle
Mycophenolic Acid
Molecular medicine
Apelin
pathway analysis
030104 developmental biology
Oncology
Gene Expression Regulation
Molecular Medicine
atherosclerosis
protein-protein interaction network
Software
Subjects
Details
- Language :
- English
- ISSN :
- 17913004 and 17912997
- Volume :
- 16
- Issue :
- 5
- Database :
- OpenAIRE
- Journal :
- Molecular Medicine Reports
- Accession number :
- edsair.doi.dedup.....a7f77f16446bf83be3e599bd8cdb974e