592 results
Search Results
2. Cyclic Voltammetric-Paper-Based Genosensor for Detection of the Target DNA of Zika Virus
- Author
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Anirudh Bishoyi, Md. Anish Alam, Mohd. Rahil Hasan, Manika Khanuja, Roberto Pilloton, and Jagriti Narang
- Subjects
Zika virus ,genosensor ,paper electrodes ,cross-reactivity ,synthetic serum ,Mechanical engineering and machinery ,TJ1-1570 - Abstract
Zika virus (ZIKV), a positive-sense single-stranded RNA virus, has been declared as the cause of a ‘worldwide public health emergency’ by the WHO since the year 2016. In cases of acute infections, it has been found to cause Guillain–Barre syndrome and microcephaly. Considering the tropical occurrence of the infections, and the absence of any proper treatments, accurate and timely diagnosis is the only way to control this infectious disease. Currently, there are many diagnostic methods under investigation by the scientific community, but they have some major limitations, such as high cost, low specificity, and poor sensitivity. To overcome these limitations, we have presented a low-cost, simple-to-operate, and portable diagnosis system for its detection by utilizing silver nanoparticles. silver nanoparticles were synthesized via chemical methods and characterization was confirmed by UV/TEM and XRD. The paper platform was synthesized using a graphene-based conductive ink, methylene blue as the redox indicator, and a portable potentiostat to perform the cyclic voltammetry to ensure true point-of-care availability for patients in remote areas.
- Published
- 2022
- Full Text
- View/download PDF
3. Towards a more precise diagnosis of hypersensitivity to beta‐lactams – an EAACI position paper
- Author
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Jean-Christoph Roger J-P Caubet, Anca-Mirela Chiriac, Paul Whitaker, Lene H. Garvey, Annick Barbaud, Alla Nakonechna, Andreas J. Bircher, Gülfem Çelik, María José Torres, Antonino Romano, Cristobalina Mayorga, Josefina Cernadas, Pascal Demoly, Knut Brockow, Marina Atanaskovic-Markovic, University of Catania [Italy], School of Medecine [Belgrade], University of Belgrade [Belgrade], CHU Tenon [AP-HP], Sorbonne Université (SU)-Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP), University Hospital Basel [Basel], Department of Dermatology and Allergy Biederstein, Technische Universität Munchen - Université Technique de Munich [Munich, Allemagne] (TUM), Geneva University Hospital (HUG), Ankara University School of Medicine [Turkey], Immunoallergy Department [Porto], Centro Hospitalar São João EPE, Faculdade de Medicina da Universidade do Porto (FMUP), Universidade do Porto, Institut Pierre Louis d'Epidémiologie et de Santé Publique (iPLESP), Sorbonne Université (SU)-Institut National de la Santé et de la Recherche Médicale (INSERM), Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier), Département pneumologie et addictologie [Montpellier], Centre Hospitalier Régional Universitaire [Montpellier] (CHRU Montpellier)-Hôpital Arnaud de Villeneuve, Department of Clinical Medicine [Copenhagen], Bispebjerg Hospital-Faculty of Health and Medical Sciences, University of Copenhagen = Københavns Universitet (KU)-University of Copenhagen = Københavns Universitet (KU), Biomedical Research Institute of Málaga (IBIMA), Universidad de Málaga [Málaga] = University of Málaga [Málaga], Allergy and Immunology Clinic [Royal Liverpool and Broadgreen University Hospital], Royal Liverpool and Broadgreen University Hospital NHS Trust, University of Liverpool, Salvy-Córdoba, Nathalie, Assistance publique - Hôpitaux de Paris (AP-HP) (AP-HP)-Sorbonne Université (SU), Universidade do Porto = University of Porto, Institut National de la Santé et de la Recherche Médicale (INSERM)-Sorbonne Université (SU), and University of Copenhagen = Københavns Universitet (UCPH)-University of Copenhagen = Københavns Universitet (UCPH)
- Subjects
Hypersensitivity, Immediate ,medicine.medical_specialty ,Allergy ,[SDV.IMM] Life Sciences [q-bio]/Immunology ,Referral ,cross-reactivity ,diagnosis ,Immunology ,Provocation test ,Drug allergy ,risk stratification ,Drug Hypersensitivity ,Beta-lactam ,03 medical and health sciences ,chemistry.chemical_compound ,Allergists ,0302 clinical medicine ,medicine ,Humans ,Immunology and Allergy ,guidelines ,Child ,Intensive care medicine ,Skin Tests ,030304 developmental biology ,0303 health sciences ,ddc:618 ,[SDV.MHEP] Life Sciences [q-bio]/Human health and pathology ,business.industry ,beta-lactams ,Novelty ,allergy ,medicine.disease ,Anti-Bacterial Agents ,3. Good health ,030228 respiratory system ,chemistry ,Risk stratification ,Position paper ,[SDV.IMM]Life Sciences [q-bio]/Immunology ,business ,[SDV.MHEP]Life Sciences [q-bio]/Human health and pathology - Abstract
International audience; A recent survey of the European Academy of Allergy and Clinical Immunology (EAACI) Drug Allergy Interest Group (DAIG) on how European allergy specialists deal with beta-lactam (BL) hypersensitivity demonstrated a significant heterogeneity in current practice, suggesting the need to review and update existing EAACI guidelines in order to make the diagnostic procedures as safe and accurate, but also as cost-effective, as possible. For this purpose, a bibliographic search on large studies regarding BL hypersensitivity diagnosis was performed by an EAACI task force, which reviewed and evaluated the literature data using the GRADE system for quality of evidence and strength of recommendation. The updated guidelines provide a risk stratification in BL hypersensitivity according to index reaction(s), as well as an algorithmic approach, based on cross-reactivity studies, in patients with a suspicion of BL hypersensitivity and an immediate need for antibiotic therapy, when referral to an allergist is not feasible. Furthermore, the update addresses availability and concentrations of skin test (ST) reagents, ST and drug provocation test (DPT) protocols, as well as diagnostic algorithms and administration of alternative BL in allergic subjects. Specifically, distinct diagnostic algorithms are suggested depending on risk stratification of the patient into high and low risk based on the morphology and chronology of the reaction, immediate (i.e., occurring within 1 to 6 hours after the last administered dose) or nonimmediate (i.e., occurring more than 1 hour after the initial drug administration), and the reaction severity. Regarding the allergy workup, the main novelty of this document is the fact that in some low-risk nonimmediate reactions ST are not mandatory, especially in children. For DPT, further studies are necessary to provide data supporting the standardization of protocols, especially of those regarding nonimmediate reactions, for which there is currently no consensus.
- Published
- 2019
4. Cyclic Voltammetric-Paper-Based Genosensor for Detection of the Target DNA of Zika Virus.
- Author
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Bishoyi, Anirudh, Alam, Md. Anish, Hasan, Mohd. Rahil, Khanuja, Manika, Pilloton, Roberto, and Narang, Jagriti
- Subjects
DNA viruses ,ZIKA virus ,SILVER nanoparticles ,METHYLENE blue ,CONDUCTIVE ink ,SINGLE-stranded DNA - Abstract
Highlights: Recent report on paper-based genosensor for the diagnosis of ZIKV target DNA. Silver nanoparticles were characterized by XRD/UV-Vis/TEM. Cross-reactivity of genosensor was deduced. Electrochemical validation was performed through CV. Zika virus (ZIKV), a positive-sense single-stranded RNA virus, has been declared as the cause of a 'worldwide public health emergency' by the WHO since the year 2016. In cases of acute infections, it has been found to cause Guillain–Barre syndrome and microcephaly. Considering the tropical occurrence of the infections, and the absence of any proper treatments, accurate and timely diagnosis is the only way to control this infectious disease. Currently, there are many diagnostic methods under investigation by the scientific community, but they have some major limitations, such as high cost, low specificity, and poor sensitivity. To overcome these limitations, we have presented a low-cost, simple-to-operate, and portable diagnosis system for its detection by utilizing silver nanoparticles. silver nanoparticles were synthesized via chemical methods and characterization was confirmed by UV/TEM and XRD. The paper platform was synthesized using a graphene-based conductive ink, methylene blue as the redox indicator, and a portable potentiostat to perform the cyclic voltammetry to ensure true point-of-care availability for patients in remote areas. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
- View/download PDF
5. Application of recombinant antigen 5 allergens from seven allergy-relevant Hymenoptera species in diagnostics.
- Author
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Schiener, M., Eberlein, B., Moreno‐Aguilar, C., Pietsch, G., Serrano, P., McIntyre, M., Schwarze, L., Russkamp, D., Biedermann, T., Spillner, E., Darsow, U., Ollert, M., Schmidt‐Weber, C. B., and Blank, S.
- Subjects
HYMENOPTERA ,BITES & stings ,ANAPHYLAXIS ,ALLERGIES ,VENOM ,PAPER wasps ,FIRE ants - Abstract
Background Hymenoptera stings can cause severe anaphylaxis in untreated venom-allergic patients. A correct diagnosis regarding the relevant species for immunotherapy is often hampered by clinically irrelevant cross-reactivity. In vespid venom allergy, cross-reactivity between venoms of different species can be a diagnostic challenge. To address immunological IgE cross-reactivity on molecular level, seven recombinant antigens 5 of the most important Vespoidea groups were assessed by different diagnostic setups. Methods The antigens 5 of yellow jackets, hornets, European and American paper wasps, fire ants, white-faced hornets, and Polybia wasps were recombinantly produced in insect cells, immunologically and structurally characterized, and their sIgE reactivity assessed by Immuno CAP, ELISA, cross-inhibition, and basophil activation test ( BAT) in patients with yellow jacket or Polistes venom allergy of two European geographical areas. Results All recombinant allergens were correctly folded and structural models and patient reactivity profiles suggested the presence of conserved and unique B-cell epitopes. All antigens 5 showed extensive cross-reactivity in sIgE analyses, inhibition assays, and BAT. This cross-reactivity was more pronounced in Immuno CAP measurements with venom extracts than in sIgE analyses with recombinant antigens 5. Dose-response curves with the allergens in BAT allowed a differentiated individual dissection of relevant sensitization. Conclusions Due to extensive cross-reactivity in various diagnostic settings, antigens 5 are inappropriate markers for differential sIgE diagnostics in vespid venom allergy. However, the newly available antigens 5 from further vespid species and the combination of recombinant allergen-based sIgE measurements with BAT represents a practicable way to diagnose clinically relevant sensitization in vespid venom allergy. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
6. History of safe exposure and bioinformatic assessment of phosphomannose-isomerase (PMI) for allergenic risk
- Author
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Mark E. Nelson, Rod A. Herman, Jason M. Roper, Henry P. Mirsky, Carey A. Mathesius, and Zhenglin Hou
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0301 basic medicine ,Allergy ,Bioinformatics ,Sequence Homology ,Genetically modified crops ,Computational biology ,Biology ,Cross Reactions ,medicine.disease_cause ,Cross-reactivity ,Zea mays ,03 medical and health sciences ,0404 agricultural biotechnology ,Allergen ,Genetics ,medicine ,Humans ,Amino Acid Sequence ,Peptide sequence ,Organism ,Selectable marker ,Plant Proteins ,Original Paper ,Mannose-6-Phosphate Isomerase ,Phosphomannose Isomerase ,PMI ,Computational Biology ,04 agricultural and veterinary sciences ,Allergens ,Plants, Genetically Modified ,040401 food science ,030104 developmental biology ,Phosphomannose-isomerase ,Animal Science and Zoology ,Safety ,Risk assessment ,Agronomy and Crop Science ,Food Hypersensitivity ,Biotechnology - Abstract
Newly expressed proteins in genetically engineered crops are evaluated for potential cross reactivity to known allergens as part of their safety assessment. This assessment uses a weight-of-evidence approach. Two key components of this allergenicity assessment include any history of safe human exposure to the protein and/or the source organism from which it was originally derived, and bioinformatic analysis identifying amino acid sequence relatedness to known allergens. Phosphomannose-isomerase (PMI) has been expressed in commercialized genetically engineered (GE) crops as a selectable marker since 2010 with no known reports of allergy, which supports a history of safe exposure, and GE events expressing the PMI protein have been approved globally based on expert safety analysis. Bioinformatic analyses identified an eight-amino-acid contiguous match between PMI and a frog parvalbumin allergen (CAC83047.1). While short amino acid matches have been shown to be a poor predictor of allergen cross reactivity, most regulatory bodies require such matches be assessed in support of the allergenicity risk assessment. Here, this match is shown to be of negligible risk of conferring cross reactivity with known allergens.
- Published
- 2021
7. Sensitisation to lipid transfer proteins in pollen – allergic adults with food allergy
- Author
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Magdalena Żbikowska-Gotz, Anna Wawrzeńczyk, Adam Wawrzeńczyk, and Zbigniew Bartuzi
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Allergy ,cross-reactivity ,lipid transfer protein ,Dermatology ,medicine.disease_cause ,Immunoglobulin E ,Cross-reactivity ,030207 dermatology & venereal diseases ,03 medical and health sciences ,0302 clinical medicine ,Oral allergy syndrome ,Food allergy ,Immunology and Allergy ,Medicine ,Internal medicine ,Artemisia vulgaris ,Original Paper ,food allergy ,biology ,business.industry ,medicine.disease ,biology.organism_classification ,RC31-1245 ,allergenic components ,RL1-803 ,Immunology ,biology.protein ,Population study ,business ,Plant lipid transfer proteins - Abstract
Introduction Food allergy affects 6-17% of Europeans and in 60% of the cases it is accompanied by inhalation allergy. This is due to two phenomena, namely co-sensitisation and cross-reactivity, which are caused by panallergens, such as lipid transfer proteins (LTP). These proteins are classified as defence proteins PR-14 and are responsible for protecting the plant from bacterial and fungal infections. Clinically, LTP allergy manifests with severe anaphylactic reactions or milder reactions, such as oral allergy syndrome. Aim To assess the incidence of specific IgE to components belonging to LTP based on molecular testing. Material and methods The study was conducted at the Department of Allergy, Clinical Immunology and Internal Diseases in Bydgoszcz, Poland. A total of 50 subjects participated in the study. Each subject had their asIgE levels determined with ImmunoCap ISAC. Results and conclusions Antigen-specific IgE antibodies to LTP components were identified in 24% of the subjects. In subjects with LTP allergy, adverse skin reactions were the most common after consumption of sensitising foods. Peach was the most common food allergen triggering these reactions. The Artemisia vulgaris component Art v 3 was the precursor of LTP allergy in our study population: the highest incidence and the highest mean levels of asIgE were demonstrated for this component.
- Published
- 2020
8. Voltammetric-based immunosensor for the detection of SARS-CoV-2 nucleocapsid antigen
- Author
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Mohammed Zourob, Shimaa Eissa, Maha Al-Mozaini, Hani A. Alhadrami, and Ahmed M. Hassan
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viruses ,Metal Nanoparticles ,02 engineering and technology ,Biosensing Techniques ,Immunosensor ,medicine.disease_cause ,01 natural sciences ,Cross-reactivity ,Analytical Chemistry ,COVID-19 Testing ,Antigen ,Limit of Detection ,Nasopharynx ,medicine ,Electrochemical detection ,Coronavirus Nucleocapsid Proteins ,Humans ,Sulfhydryl Compounds ,Voltammetry ,Electrodes ,Antibody ,Detection limit ,Nucleocapsid protein ,Immunoassay ,Original Paper ,Chromatography ,medicine.diagnostic_test ,Chemistry ,SARS-CoV-2 ,010401 analytical chemistry ,Fatty Acids ,virus diseases ,COVID-19 ,Electrochemical Techniques ,021001 nanoscience & nanotechnology ,Phosphoproteins ,Carbon ,0104 chemical sciences ,Linear range ,Colloidal gold ,Gold ,0210 nano-technology ,Biosensor ,Antibodies, Immobilized - Abstract
Since the COVID-19 disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS- CoV-2) was declared a pandemic, it has spread rapidly, causing one of the most serious outbreaks in the last century. Reliable and rapid diagnostic tests for COVID-19 are crucial to control and manage the outbreak. Here, a label-free square wave voltammetry-based biosensing platform for the detection of SARS-CoV-2 in nasopharyngeal samples is reported. The sensor was constructed on screen-printed carbon electrodes coated with gold nanoparticles. The electrodes were functionalized using 11-mercaptoundecanoic acid (MUA) which was used for the immobilization of an antibody against SARS-CoV-2 nucleocapsid protein (N protein). The binding of the immunosensor with the N protein caused a change in the electrochemical signal. The detection was realised by measuring the change in reduction peak current of a redox couple using square wave voltammetry at 0.04 V versus Ag ref. electrode on the immunosensor upon binding with the N protein. The electrochemical immunosensor showed high sensitivity with a linear range from 1.0 pg.mL−1 to 100 ng.mL−1 and a limit of detection of 0.4 pg.mL−1 for the N protein in PBS buffer pH 7.4. Moreover, the immunosensor did not exhibit significant response with other viruses such as HCoV, MERS-CoV, Flu A and Flu B, indicating the high selectivity of the sensor for SARS-CoV-2. However, cross reactivity of the biosensor with SARS-CoV is indicated, which gives ability of the sensor to detect both SARS-CoV and SARS-CoV-2. The biosensor was successfully applied to detect the SARS-CoV-2 virus in clinical samples showing good correlation between the biosensor response and the RT-PCR cycle threshold values. We believe that the capability of miniaturization, low-cost and fast response of the proposed label-free electrochemical immunosensor will facilitate the point-of-care diagnosis of COVID 19 and help prevent further spread of infection.
- Published
- 2021
9. A ten-year study of immunogenicity and safety of the AS04-HPV-16/18 vaccine in adolescent girls aged 10-14 years
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Tino F. Schwarz, Sylviane Poncelet, Li-Min Huang, Lan Lin, Naveen Karkada, Alejandra Valencia, Falko Panzer, Nicolas Folschweiller, Gary Dubin, Frank Struyf, Cheng-Hsun Chiu, and Annabelle Decreux
- Subjects
safety ,Time Factors ,cross-reactivity ,Adolescent ,viruses ,HPV-31 ,030231 tropical medicine ,Immunology ,HPV-16/18 AS04-adjuvanted vaccine ,Uterine Cervical Neoplasms ,Antibodies, Viral ,complex mixtures ,03 medical and health sciences ,0302 clinical medicine ,Immunogenicity, Vaccine ,Adjuvants, Immunologic ,Immunology and Allergy ,Medicine ,Humans ,030212 general & internal medicine ,Papillomavirus Vaccines ,Human papillomavirus ,Child ,Pharmacology ,Human papillomavirus 16 ,Human papillomavirus 18 ,business.industry ,Immunogenicity ,Papillomavirus Infections ,Vaccination ,virus diseases ,HPV-45 ,immunogenicity persistence ,Female ,business ,predictive modeling ,non-vaccine types ,Research Paper ,Follow-Up Studies - Abstract
This study assessed long-term immunogenicity and safety following 3 doses of AS04-adjuvanted human papillomavirus (HPV)-16/18 L1 virus-like particle (VLP) vaccine in females 10–14 years old. Girls included in the immunogenicity subset in the primary controlled, observer-blinded, randomized study (NCT00196924) who received 3 doses were invited for a 10-year follow-up (NCT00316706 and NCT00877877). Serum antibody responses against HPV-16/18 (vaccine types) and HPV-31/45 (non-vaccine types) were measured by enzyme-linked immunosorbent assay (ELISA) using type-specific VLP as coating antigens. Serious adverse events (SAEs) and pregnancy information were recorded. At Month (M) 120, all subjects (N = 418, according-to-protocol immunogenicity cohort) were seropositive for anti-HPV-16/18 antibodies. Geometric mean titers (GMTs) were 1589.9 ELISA Units [EU]/mL (95% confidence interval [CI]: 1459.8–1731.6) for anti-HPV-16 and 597.2 EU/mL (95% CI: 541.7–658.5) for anti-HPV-18 in subjects seronegative at baseline for the type analyzed. Post hoc mathematical modeling predicted a durability ≥50 years for anti-HPV-16 and anti-HPV-18. For the non-vaccine humoral type response, all initially seronegative subjects had seroconverted at M7, with anti-HPV-31 GMT of 2030.5 EU/mL (95% CI: 1766.2–2334.4) and anti-HPV-45 GMT of 2300.8 EU/mL (95% CI: 2036.8–2599.0). At M120, 87.7% and 85.1% remained seropositive for anti-HPV-31 with GMT of 242.9 EU/mL (95% CI: 201.4–293.0) and anti-HPV-45 with GMT of 204.7 EU/mL (95% CI: 170.0–246.6). During the 10-year follow-up, no SAEs or abnormal pregnancy outcomes were causally related to vaccination. Three doses of the AS04-HPV-16/18 vaccine induced high and sustained antibody response against HPV-16,18,31 and 45 in girls aged 10–14 years during the 10-year follow-up, with an acceptable long-term safety profile.
- Published
- 2019
10. Genomics of Shrimp Allergens and Beyond.
- Author
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Li, Shanshan, Chu, Ka Hou, and Wai, Christine Yee Yan
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ALLERGENS ,SHELLFISH ,SHRIMPS ,IMMUNOGLOBULIN E ,TECHNOLOGICAL innovations ,GENOMICS ,ALLERGIES - Abstract
Allergy to shellfishes, including mollusks and crustaceans, is a growing health concern worldwide. Crustacean shellfish is one of the "Big Eight" allergens designated by the U.S. Food and Drug Administration and is the major cause of food-induced anaphylaxis. Shrimp is one of the most consumed crustaceans triggering immunoglobulin E (IgE)-mediated allergic reactions. Over the past decades, the allergen repertoire of shrimp has been unveiled based on conventional immunodetection methods. With the availability of genomic data for penaeid shrimp and other technological advancements like transcriptomic approaches, new shrimp allergens have been identified and directed new insights into their expression levels, cross-reactivity, and functional impact. In this review paper, we summarize the current knowledge on shrimp allergens, as well as allergens from other crustaceans and mollusks. Specific emphasis is put on the genomic information of the shrimp allergens, their protein characteristics, and cross-reactivity among shrimp and other organisms. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
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11. The role of cross-reactive immunity to emerging coronaviruses: Implications for novel universal mucosal vaccine design.
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Alturaiki, Wael
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CORONAVIRUSES ,COMMON cold ,BACTERIAL vaccines ,TALL-1 (Protein) ,COVID-19 pandemic ,IMMUNITY ,VACCINES - Abstract
Copyright of Saudi Medical Journal is the property of Saudi Medical Journal and its content may not be copied or emailed to multiple sites or posted to a listserv without the copyright holder's express written permission. However, users may print, download, or email articles for individual use. This abstract may be abridged. No warranty is given about the accuracy of the copy. Users should refer to the original published version of the material for the full abstract. (Copyright applies to all Abstracts.)
- Published
- 2023
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- View/download PDF
12. Single dose vaccination of the ASO3-adjuvanted A(H1N1)pdm09 monovalent vaccine in health care workers elicits homologous and cross-reactive cellular and humoral responses to H1N1 strains
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John Wood, Rebecca Jane Cox, Åsne Jul-Larsen, Rishi D. Pathirana, Emanuele Montomoli, Sarah Lartey, and Fan Zhou
- Subjects
Male ,Hemagglutination Inhibition Tests ,pandemic influenza ,Time Factors ,cross-reactivity ,alpha-Tocopherol ,Polysorbates ,medicine.disease_cause ,Antibodies, Viral ,Cross-reactivity ,AS03 ,0302 clinical medicine ,Influenza A Virus, H1N1 Subtype ,Immunologic ,Influenza A Virus ,Immunology and Allergy ,Interferon gamma ,Viral ,030212 general & internal medicine ,Memory B cell ,0303 health sciences ,Immunity, Cellular ,ELISPOT ,H1N1 ,Humoral ,virus diseases ,Middle Aged ,3. Good health ,Vaccination ,Drug Combinations ,Influenza Vaccines ,HCW ,Tumor necrosis factor alpha ,Female ,Human ,medicine.drug ,Research Paper ,Adult ,Squalene ,Health Personnel ,Immunology ,Cross Reactions ,Antibodies ,03 medical and health sciences ,Interferon-gamma ,Adjuvants, Immunologic ,Influenza, Human ,medicine ,Humans ,H1N1 Subtype ,Adjuvants ,Immunization Schedule ,030304 developmental biology ,Pharmacology ,business.industry ,healthcare workers ,Immunity ,Healthcare workers ,Immune responses ,Pandemic influenza ,Immunity, Humoral ,vaccination ,Virology ,Influenza ,immune responses ,Cellular ,business - Abstract
Healthcare workers (HCW) were prioritized for vaccination during the 2009 influenza A(H1N1)pdm09 pandemic. We conducted a clinical trial in October 2009 where 237 HCWs were immunized with a AS03-adjuvanted A(H1N1)pdm09 monovalent vaccine. In the current study, we analyzed the homologous and cross-reactive H1N1 humoral responses using prototype vaccine strains dating back to 1977 by the haemagglutinin inhibition (HI), single radial hemolysis SRH), antibody secreting cell (ASC) and memory B cell (MBC) assays. The cellular responses were assessed by interferon-γ (IFN-γ) ELISPOT and by intracellular staining (ICS) for the Th1 cytokines IFN-γ, interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α). All assays were performed using blood samples obtained prior to (day 0) and 7, 14 and 21 d post-pandemic vaccination, except for ASC (day 7) and ICS (days 0 and 21). Vaccination elicited rapid HI, SRH and ASC responses against A(H1N1)pdm09 which cross reacted with seasonal H1N1 strains. MBC responses were detected against the homologous and seasonal H1N1 strains before vaccination and were boosted 2 weeks post-vaccination. An increase in cellular responses as determined by IFN-γ ELISPOT and ICS were observed 1–3 weeks after vaccination. Collectively, our data show that the AS03-adjuvanted A(H1N1)pdm09 vaccine induced rapid cellular and humoral responses against the vaccine strain and the response cross-reacted against prototype H1N1 strains dating back to 1977. publishedVersion
- Published
- 2015
13. A simple and rapid immunochromatographic test kit for rabies diagnosis
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Kazuyo Goto, Pakamatz Khawplod, Kumato Mifune, Katsuyoshi Takayama, Yukuharu Kobayashi, Kamruddin Ahmed, Veera Tepsumethanon, Kinjiro Morimoto, Akira Nishizono, Chanarong Mitmoonpitak, Kazuaki Mannen, Takehito Yasui, Seiji Shiota, and Satoshi Inoue
- Subjects
medicine.drug_class ,Rabies ,Immunology ,Biology ,medicine.disease_cause ,Monoclonal antibody ,Antibodies, Viral ,Microbiology ,Cross-reactivity ,Immunoenzyme Techniques ,Dogs ,Virology ,medicine ,Animals ,Direct fluorescent antibody ,Antigens, Viral ,rapid diagnosis ,Editor Communicated Paper ,immunochromatographic test ,Rabies virus ,Antibodies, Monoclonal ,Gold standard (test) ,medicine.disease ,Nucleoprotein ,Nucleoproteins ,biology.protein ,Reagent Kits, Diagnostic ,Antibody - Abstract
In rabies endemic countries, funds and infrastructure are often insufficient to employ the approved gold standard for the definitive diagnosis of rabies: the direct fluorescent test. In the present study, two types (type 1 and 2) of an ICT kit were evaluated for detection of rabies. These were developed using monoclonal antibodies which recognize epitope II and III of the nucleoprotein of rabies virus. Both kits specifically detected all rabies virus strains and there was no cross reactivity with Lyssaviruses (Lagos, Mokola and Duvenhage), Rhabdovirus (VSV and Oita 296/1972) and other common canine‐pathogenic viruses. In type 1, a single type of monoclonal antibody was used. It was capable of detecting recombinant nucleoprotein and showed sensitivity of 95.5% (42/44) and specificity of 88.9% (32/36) using brain samples from rabid dogs. In contrast, type 2 which was made of two different monoclonal antibodies had a lower sensitivity of 93.2% (41/44) and higher specificity of 100% (36/36). These ICT kits provide a simple and rapid method for rabies detection. They need neither cold chain for transportation nor complicated training for personnel. This diagnostic test is suitable for rabies screening, particularly in areas with a high prevalence of rabies and where the fluorescent antibody test is not available.
- Published
- 2008
14. Clinical Use of the ImmunoCAP Inhibition Test in the Diagnosis of Meat Allergy Caused by a Tick Bite in an Adult Male with No Previous Atopic History.
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Lis, Kinga, Ukleja-Sokołowska, Natalia, Karwowska, Kornelia, Wernik, Joanna, Pawłowska, Małgorzata, and Bartuzi, Zbigniew
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FOOD allergy ,ALLERGIES ,DIAGNOSIS ,POULTRY as food ,ALLERGENS ,IMMUNOGLOBULINS ,VENOM ,IMMUNOGLOBULIN E - Abstract
(1) Background: alpha-gal syndrome (AGS) is a serious, potentially life-threatening allergic reaction. This is a type of food allergy to red meat and other mammalian products (e.g., gelatin). In Poland, this problem seems to be rare or, more likely, very underdiagnosed. The diagnosis of AGS is difficult. It seems that the knowledge about this syndrome is insufficient. There are no effective diagnostic tools able to clearly diagnose this cross-reactive allergy. This paper presents the clinical application of a non-standard method in the diagnosis of a cross-reactive allergy using the example of AGS. (2) Methods: standard tests for in vitro allergy diagnostics and the non-standard ImmunoCAP inhibition test(IT) were carried out for serum collected from a patient with a red meat allergy. (3) Results: the serum concentration of anti-α-Gal IgE was very high (302 kUA/L), and IgE antibodies toanti-mammalian-meat allergens were found. The level of IgE antibodies to mammalian meat allergens decreased after blocking on α-GAL-CAP. The concentration of anti-α-Gal IgE decreased after blocking on CAPs coated with various mammalian meat allergens. Blocking with allergens of poultry meat did not affect the concentration of anti-α-Gal IgE. (4) Conclusions: the ImmunoCAP ITseems to be a useful tool in the diagnosis of cross-reactive allergies. Based on their clinical history and test results, the patient was diagnosed with AGS caused by a primary sensitization to α-Gal after a tick bite. This is the second case of AGS described in Poland and the first in Pomerania. [ABSTRACT FROM AUTHOR]
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- 2023
- Full Text
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15. Cross reactivity of serum antibody responses elicited by DNA vaccines expressing HA antigens from H1N1 subtype influenza vaccines in the past 30 years
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Shan Lu, Huaiqing Chen, Iman Almansour, and Shixia Wang
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Immunology ,Hemagglutinin (influenza) ,Hemagglutinin Glycoproteins, Influenza Virus ,Cross Reactions ,medicine.disease_cause ,Antibodies, Viral ,H5N1 genetic structure ,Cross-reactivity ,Virus ,DNA vaccination ,Influenza A Virus, H1N1 Subtype ,Antigen ,medicine ,Vaccines, DNA ,Immunology and Allergy ,Animals ,Pharmacology ,biology ,Virology ,Vaccination ,Influenza Vaccines ,biology.protein ,Rabbits ,Antibody ,Research Paper - Abstract
In the past three decades, ten H1 subtype influenza vaccines have been recommended for global seasonal flu vaccination. Some of them were used only for one year before being replaced by another H1 flu vaccine while others may be used for up to seven years. While the selection of a new seasonal flu vaccine was based on the escape of a new emerging virus that was not effectively protected by the existing flu formulation, there is limited information on the magnitude and breadth of cross reactivity among H1 subtype virus circulation over a long period. In the current study, HA-expressing DNA vaccines were constructed to express individual HA antigens from H1 subtype vaccines used in the past 30 y. Rabbits naive to HA antibody responses were immunized with these HA DNA vaccines and the cross reactivity of these sera against HA antigen and related H1 viruses in the same period was studied. Our data indicate that the level of cross reactivity was different for different viral isolates and the key mutations responsible for the cross reactivity may involve only a limited number of residues. Our results provide useful information for the development of improved seasonal vaccines than can achieve broad protection against viruses within the same H1 subtype.
- Published
- 2013
16. Influence of spironolactone on neonatal screening for congenital adrenal hyperplasia
- Author
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Kimiaki Yamano, Itaru Terai, Junri Arai, Naoshi Ichihara, and Kunihiko Kobayashi
- Subjects
Male ,medicine.medical_specialty ,medicine.medical_treatment ,Dose dependence ,Enzyme-Linked Immunosorbent Assay ,Spironolactone ,medicine.disease_cause ,High-performance liquid chromatography ,Cross-reactivity ,chemistry.chemical_compound ,Neonatal Screening ,Internal medicine ,Medicine ,Humans ,Congenital adrenal hyperplasia ,False Positive Reactions ,Diuretics ,Mass screening ,Adrenal Hyperplasia, Congenital ,business.industry ,17-alpha-Hydroxyprogesterone ,Infant, Newborn ,Obstetrics and Gynecology ,General Medicine ,Original Articles ,medicine.disease ,Endocrinology ,chemistry ,Blood filter paper ,Pediatrics, Perinatology and Child Health ,Diuretic ,business - Abstract
AIM To determine if the diuretic spironolactone cross reacts with 17α-hydroxyprogesterone (17OHP) in an enzyme linked immunosorbent assay (ELISA) kit used for the mass screening of congenital adrenal hyperplasia. METHODS Concentrations of 17OHP on a blood filter paper disc were measured using an ELISA kit (kit C-7: ENZAPLATE N-17α -OHP-7; Chiron, Tokyo, Japan). The cross reactivity of spironolactone and its metabolites with 17OHP was determined. The concentrations of spironolactone and its metabolites in blood were measured using HPLC (high performance liquid chromatography). RESULTS Spironolactone cross reacted with 17OHP using kit C-7 (0.01%), by increasing 17OHP concentration in a dose dependent manner. The blood concentration of spironolactone and its metabolites was nearly 900 ng/ml, high enough to show an additive effect on the 17OHP concentration. About 12% of the false positive cases screened using the kit were due to the administration of spironolactone. CONCLUSIONS Spironolactone interferes with 17OHP concentrations, leading to false positive test results for CAH.
- Published
- 1999
17. In vivo and T Cell Cross-Reactivity between Walnut, Cashew and Peanut
- Author
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Michael D. Kulis, A. Wesley Burks, and Laurent Pons
- Subjects
Arachis ,T cell ,T-Lymphocytes ,Immunology ,Juglans ,Cross Reactions ,Immunoglobulin E ,medicine.disease_cause ,Cross-reactivity ,Mice ,In vivo ,Food allergy ,medicine ,Immunology and Allergy ,Animals ,Humans ,Anacardium ,Plant Proteins ,Original Paper ,B-Lymphocytes ,Mice, Inbred C3H ,biology ,digestive, oral, and skin physiology ,Seed Storage Proteins ,Cross reactions ,food and beverages ,General Medicine ,Allergens ,medicine.disease ,In vitro ,Disease Models, Animal ,medicine.anatomical_structure ,Immunoglobulin G ,Vicilin ,biology.protein ,Female ,Food Hypersensitivity - Abstract
Background: Examination of IgE cross-reactivity among nuts has been limited to in vitro experiments. Cross-reactivity studies of nuts at the T cell level are difficult to interpret because of the inability to determine which cellular responses are from a true sensitization and which are due to cross-reactivity. Using a mouse model in which the sensitizing nuts are controlled may provide novel methods to investigate in vivo and T cell cross-reactivity. Methods: C3H/HeJ mice were sensitized by intraperitoneal injection of cashew alone (monosensitized mice), or cashew plus walnut, utilizing alum as an adjuvant. Both groups underwent challenges to cashew, walnut and peanut, with subsequent monitoring of anaphylactic reactions. Anaphylactic antibodies were quantified by ELISA, and protein allergens were identified by Western blotting. Cellular responses were studied via splenocyte proliferation assay and measurement of secreted cytokines. Results: The monosensitized mice reacted to cashew and walnut during challenges, with significantly weaker reactions induced on challenge with peanut. Cross-reactive IgE to walnut and peanut were detected by ELISA, and the cross-reactive allergens were identified as vicilin proteins. In cellular assays, splenocytes from the monosensitized mice proliferated and produced IL-4 and IL-5 in response to cashew, walnut and peanut. The cashew- plus walnut-sensitized mice experienced stronger clinical reactions to walnut, recognized additional walnut allergens and secreted significantly more IL-4 and IL-5 in walnut-stimulated splenocyte assays compared to the monosensitized mice. Conclusions: Cross-reactivity in vivo was found between cashew and walnut, while cross-reactivity among cashew, walnut and peanut was demonstrated at the T cell level.
- Published
- 2008
18. The Anti-Glucocorticoid Receptor Antibody Clone 5E4: Raising Awareness of Unspecific Antibody Binding.
- Author
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Ehlers, Lisa, Kirchner, Marieluise, Mertins, Philipp, Strehl, Cindy, Buttgereit, Frank, and Gaber, Timo
- Subjects
RECEPTOR antibodies ,MOLECULAR cloning ,IMMUNOGLOBULINS ,TRANSCRIPTION factors ,GLUCOCORTICOID receptors ,MONOCLONAL antibodies - Abstract
Unspecific antibody binding takes a significant toll on researchers in the form of both the economic burden and the disappointed hopes of promising new therapeutic targets. Despite recent initiatives promoting antibody validation, a uniform approach addressing this issue has not yet been developed. Here, we demonstrate that the anti-glucocorticoid receptor (GR) antibody clone 5E4 predominantly targets two different proteins of approximately the same size, namely AMP deaminase 2 (AMPD2) and transcription intermediary factor 1-beta (TRIM28). This paper is intended to generate awareness of unspecific binding of well-established reagents and advocate the use of more rigorous verification methods to improve antibody quality in the future. [ABSTRACT FROM AUTHOR]
- Published
- 2022
- Full Text
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19. Quality Issues of Research Antibodies.
- Author
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Weller, Michael G.
- Subjects
IMMUNOGLOBULINS ,FOOD chemistry ,DIAGNOSTIC imaging ,IMMUNOCHEMISTRY ,QUALITY control - Abstract
According to several recent studies, an unexpectedly high number of landmark papers seem to be not reproducible by independent laboratories. Nontherapeutic antibodies used for research, diagnostic, food analytical, environmental, and other purposes play a significant role in this matter. Although some papers have been published offering suggestions to improve the situation, they do not seem to be comprehensive enough to cover the full complexity of this issue. In addition, no obvious improvements could be noticed in the field as yet. This article tries to consolidate the remarkable variety of conclusions and suggested activities into a more coherent conception. It is concluded that funding agencies and journal publishers need to take first and immediate measures to resolve these problems and lead the way to a more sustainable way of bioanalytical research, on which all can rely with confidence. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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20. Revisiting Latex-Fruit Syndrome after 30 Years of Research: A Comprehensive Literature Review and Description of Two Cases.
- Author
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Gromek, Weronika, Kołdej, Natalia, Świtała, Szymon, Majsiak, Emilia, and Kurowski, Marcin
- Subjects
LITERATURE reviews ,SYMPTOMS ,RUBBER ,PAPAYA ,ALLERGENS - Abstract
Thirty years have passed since the concept of latex-fruit syndrome (LFS) was first introduced. Since then, this phenomenon, characterized by cross-reactivity between natural latex rubber allergens and certain fruit allergens, has been extensively studied. This literature review sought to determine the prevalence of LFS in latex-allergic patients, identify the most common cross-reactions with fruit allergens in individuals with LFS, and understand the clinical manifestations of this syndrome. An extensive literature search was carried out using PubMed and Scopus databases, while applying the Preferred Reporting Items for Systematic Reviews and Meta-Analysis methodology. The analysis of original studies revealed a wide variation in LFS prevalence (4–88%) influenced by diverse diagnostic tools, different geographical regions, and the size of study populations. Our findings indicate that the most prevalent allergenic fruits in patients with LFS are banana, avocado, kiwifruit, and papaya. After evaluating the symptoms of the fruit hypersensitivity of patients with LFS, the clinical manifestation of hypersensitivity constituted 73% of systemic allergy symptoms and only 27% of reported symptoms described the localized allergy. Furthermore, the clinical picture of latex-fruit syndrome is illustrated through two cases, one typical and one with an unusual presentation. Their clinical features were assessed and contrasted utilizing different anaphylaxis severity grading criteria. To properly manage LFS, it is essential to establish standardized diagnostic criteria and severity grading systems, as these are crucial for accurate diagnosis and effective treatment. [ABSTRACT FROM AUTHOR]
- Published
- 2024
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- View/download PDF
21. Neglected but Clinically Relevant Allergens in Korea
- Author
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Jeong, Kyoung Yong and Park, Jung-Won
- Published
- 2024
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22. Adverse reaction to Ficus Carica: reported case of a possible cross-reactivity with Der p1
- Author
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Urbani, S., Aruanno, A., and Nucera, E.
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- 2020
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23. Impact of Enolase in Allergic Disease
- Author
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Morales-Amparano, Martha Beatriz, Teran, Maria G, Huerta-Ocampo, José Ángel, and Teran, Luis M
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- 2024
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24. Diagnosing and managing patients with drug hypersensitivity.
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Fernandez, Javier and Doña, Inmaculada
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DRUG side effects ,SKIN tests ,IDIOSYNCRATIC drug reactions ,VIRUS diseases ,DIAGNOSIS - Abstract
Introduction: Diagnosing and managing drug hypersensitivity is challenging because there are no clear limits between different types of drug reactions. Distinguishing between type A (predictable) and type B (hypersensitivity) reactions when a drug is introduced on the market is not easy. When many people use a drug, adverse reactions can occur, conditioned by diverse genetic profiles, viral infections or concomitant therapy. Occasionally the only tool clinicians have on which to base the diagnosis is the clinical history. Skins tests orin vitrotests sometimes have low sensitivity or are unavailable, and drug provocation tests may be dangerous or strictly forbidden in case of severe cutaneous reactions. Areas covered: This paper reviews the diagnosis and management of the two main types of immunological reactions: IgE-mediated immediate drug hypersensitivity reactions (IDHRs) and non-immediate drug hypersensitivity reactions (NIDHRs). Expert commentary: Although Europe and the United States use different diagnostic methods, patients with history of drug hypersensitivity must avoid the suspicious drug, and clinicians must assess tolerance to safe alternatives under medical surveillance. Sometimes desensitization may be required. There is a consensus about the need to perform genetic testing for specific drugs and give patients proper documentation to prevent future exposure to culprit drugs. [ABSTRACT FROM AUTHOR]
- Published
- 2018
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25. A highly effective ferritin-based divalent nanoparticle vaccine shields Syrian hamsters against lethal Nipah virus.
- Author
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Chunhong Yin, Yan Feng Yao, Peipei Yang, Hang Liu, Ge Gao, Yun Peng, Miaoyu Chen, Mingqing Lu, Xuekai Zhang, Weiwei Guo, Zihan Zhang, Xue Hu, Zhiming Yuan, and Chao Shan
- Subjects
NIPAH virus ,GOLDEN hamster ,NANOPARTICLES ,RABIES virus ,PARAMYXOVIRUSES ,HENIPAVIRUSES ,VACCINES ,AUJESZKY'S disease virus - Abstract
The Nipah virus (NiV), a highly deadly bat-borne paramyxovirus, poses a substantial threat due to recurrent outbreaks in specific regions, causing severe respiratory and neurological diseases with high morbidity. Two distinct strains, NiV-Malaysia (NiV-M) and NiV-Bangladesh (NiV-B), contribute to outbreaks in different geographical areas. Currently, there are no commercially licensed vaccines or drugs available for prevention or treatment. In response to this urgent need for protection against NiV and related henipaviruses infections, we developed a novel homotypic virus-like nanoparticle (VLP) vaccine co-displaying NiV attachment glycoproteins (G) from both strains, utilizing the self-assembling properties of ferritin protein. In comparison to the NiV G subunit vaccine, our nanoparticle vaccine elicited significantly higher levels of neutralizing antibodies and provided complete protection against a lethal challenge with NiV infection in Syrian hamsters. Remarkably, the nanoparticle vaccine stimulated the production of antibodies that exhibited superior cross-reactivity to homologous or heterologous henipavirus. These findings underscore the potential utility of ferritin-based nanoparticle vaccines in providing both broad-spectrum and long-term protection against NiV and emerging zoonotic henipaviruses challenges. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
26. Utilizing the Banana S-Adenosyl-L-Homocysteine Hydrolase Allergen to Identify Cross-Reactive IgE in Ryegrass-, Latex-, and Kiwifruit-Allergic Individuals.
- Author
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Đurašinović, Tatjana, Lopandić, Zorana, Protić-Rosić, Isidora, Ravnsborg, Tina, Blagojević, Gordan, Burazer, Lidija, Jensen, Ole N., and Gavrović-Jankulović, Marija
- Subjects
BANANAS ,FOOD allergy ,ALLERGENS ,RYEGRASSES ,AMINO acid sequence ,IMMUNOGLOBULIN E ,ESCHERICHIA coli ,POLLINATION ,FOOD safety - Abstract
Food allergies mediated by specific IgE (sIgE) have a significant socioeconomic impact on society. Evaluating the IgE cross-reactivity between allergens from different allergen sources can enable the better management of these potentially life-threatening adverse reactions to food proteins and enhance food safety. A novel banana fruit allergen, S-adenosyl-L-homocysteine hydrolase (SAHH), has been recently identified and its recombinant homolog was heterologously overproduced in E. coli. In this study, we performed a search in the NCBI (National Center for Biotechnology Information) for SAHH homologs in ryegrass, latex, and kiwifruit, all of which are commonly associated with pollen-latex-fruit syndrome. In addition, Western immunoblot analysis was utilized to identify the cross-reactive IgE to banana SAHH in the sera of patients with a latex allergy, kiwifruit allergy, and ryegrass allergy. ClustalOmega analysis showed more than 92% amino acid sequence identity among the banana SAHH homologs in ryegrass, latex, and kiwifruit. In addition to five B-cell epitopes, in silico analysis predicted eleven T-cell epitopes in banana SAHH, seventeen in kiwifruit SAHH, twelve in ryegrass SAHH, and eight in latex SAHH, which were related to the seven-allele HLA reference set (HLA-DRB1*03:01, HLA-DRB1*07:01, HLA-DRB1*15:01, HLA-DRB3*01:01, HLA-DRB3*02:02, HLA-DRB4*01:01, HLA-DRB5*01:01). Four T-cell epitopes were identical in banana and kiwifruit SAHH (positions 328, 278, 142, 341), as well as banana and ryegrass SAHH (positions 278, 142, 96, and 341). All four SAHHs shared two T-cell epitopes (positions 278 and 341). In line with the high amino acid sequence identity and B-cell epitope homology among the analyzed proteins, the cross-reactive IgE to banana SAHH was detected in three of three latex-allergic patients, five of six ryegrass-allergic patients, and two of three kiwifruit-allergic patients. Although banana SAHH has only been studied in a small group of allergic individuals, it is a novel cross-reactive food allergen that should be considered when testing for pollen-latex-fruit syndrome. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
27. IgE-Mediated Allergy and Asymptomatic Sensitization to Cannabis Allergens—Review of Current Knowledge and Presentation of Six Cases.
- Author
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Wąsik, Jakub, Likońska, Aleksandra, and Kurowski, Marcin
- Subjects
ALLERGENS ,ALLERGIES ,ITCHING ,ANAPHYLAXIS ,MEDICAL personnel ,POLLEN - Abstract
Cannabis allergy is a relatively new phenomenon described in the 1970s. Its increased frequency has been observed over the last years due to the increasing therapeutic and recreational use of cannabis-based products. Sensitization possibly leading to allergy symptoms can occur not only through the smoking of cannabis, but also through ingestion, the inhalation of pollen, or direct contact. The severity of symptoms varies from benign pruritus to anaphylaxis. There is scant information available to support clinicians throughout the entire therapeutic process, starting from diagnosis and ending in treatment. In this review, we present six cases of patients in whom molecular in vitro testing revealed sensitization to cannabis extract and/or cannabis-derived nsLTP molecules (Can s 3). Based on these cases, we raise important questions regarding this topic. The article discusses current proposals and highlights the importance of further research not only on cannabis allergy but also on asymptomatic sensitization to cannabis allergens, which may be ascertained in some percentage of the population. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
28. Enhancement of NETosis by ACE2-cross-reactive anti-SARS-CoV-2 RBD antibodies in patients with COVID-19.
- Author
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Hsieh, Kun-Han, Chao, Chiao-Hsuan, Cheng, Yi-Ling, Lai, Yen-Chung, Chuang, Yung-Chun, Wang, Jen-Ren, Chang, Sui-Yuan, Hung, Yuan-Pin, Chen, Yi-Ming Arthur, Liu, Wei-Lun, Chuang, Woei-Jer, and Yeh, Trai-Ming
- Subjects
COVID-19 ,CORONAVIRUS spike protein ,PROTEIN domains ,PROGNOSIS ,FC receptors ,CORONAVIRUS diseases - Abstract
Background: High levels of neutrophil extracellular trap (NET) formation or NETosis and autoantibodies are related to poor prognosis and disease severity of COVID-19 patients. Human angiotensin-converting enzyme 2 (ACE2) cross-reactive anti-severe acute respiratory syndrome coronavirus 2 spike protein receptor-binding domain (SARS-CoV-2 RBD) antibodies (CR Abs) have been reported as one of the sources of anti-ACE2 autoantibodies. However, the pathological implications of CR Abs in NET formation remain unknown. Methods: In this study, we first assessed the presence of CR Abs in the sera of COVID-19 patients with different severity by serological analysis. Sera and purified IgG from CR Abs positive COVID-19 patients as well as a mouse monoclonal Ab (mAb 127) that can recognize both ACE2 and the RBD were tested for their influence on NETosis and the possible mechanisms involved were studied. Results: An association between CR Abs levels and the severity of COVID-19 in 120 patients was found. The CR Abs-positive sera and IgG from severe COVID-19 patients and mAb 127 significantly activated human leukocytes and triggered NETosis, in the presence of RBD. This NETosis, triggered by the coexistence of CR Abs and RBD, activated thrombus-related cells but was abolished when the interaction between CR Abs and ACE2 or Fc receptors was disrupted. We also revealed that CR Abs-induced NETosis was suppressed in the presence of recombinant ACE2 or the Src family kinase inhibitor, dasatinib. Furthermore, we found that COVID-19 vaccination not only reduced COVID-19 severity but also prevented the production of CR Abs after SARS-CoV-2 infection. Conclusions: Our findings provide possible pathogenic effects of CR Abs in exacerbating COVID-19 by enhancing NETosis, highlighting ACE2 and dasatinib as potential treatments, and supporting the benefit of vaccination in reducing disease severity and CR Abs production in COVID-19 patients. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
29. Vaccine- and natural infection-induced mechanisms that could modulate vaccine safety
- Author
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Ronald N. Kostoff, Darja Kanduc, Alan L. Porter, Yehuda Shoenfeld, Daniela Calina, Michael B. Briggs, Demetrios A. Spandidos, and Aristidis Tsatsakis
- Subjects
COVID-19 pandemic ,Vaccine safety ,Autoimmunity ,Molecular mimicry ,Cross-Reactivity ,Immune interference ,Toxicology. Poisons ,RA1190-1270 - Abstract
A degraded/dysfunctional immune system appears to be the main determinant of serious/fatal reaction to viral infection (for COVID-19, SARS, and influenza alike). There are four major approaches being employed or considered presently to augment or strengthen the immune system, in order to reduce adverse effects of viral exposure. The three approaches that are focused mainly on augmenting the immune system are based on the concept that pandemics/outbreaks can be controlled/prevented while maintaining the immune-degrading lifestyles followed by much of the global population. The fourth approach is based on identifying and introducing measures aimed at strengthening the immune system intrinsically in order to minimize future pandemics/outbreaks.Specifically, the four measures are: 1) restricting exposure to virus; 2) providing reactive/tactical treatments to reduce viral load; 3) developing vaccines to prevent, or at least attenuate, the infection; 4) strengthening the immune system intrinsically, by a) identifying those factors that contribute to degrading the immune system, then eliminating/reducing them as comprehensively, thoroughly, and rapidly as possible, and b) replacing the eliminated factors with immune-strengthening factors.This paper focuses on vaccine safety. A future COVID-19 vaccine appears to be the treatment of choice at the national/international level. Vaccine development has been accelerated to achieve this goal in the relatively near-term, and questions have arisen whether vaccine safety has been/is being/will be compromised in pursuit of a shortened vaccine development time. There are myriad mechanisms related to vaccine-induced, and natural infection-induced, infections that could adversely impact vaccine effectiveness and safety. This paper summarizes many of those mechanisms.
- Published
- 2020
- Full Text
- View/download PDF
30. Immunologic studies on nucleic acids and their components. I. An analysis of the specificity of anti-deoxyadenylate antibodies by a membrane-binding technique
- Author
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T.M. Jacob and M.Z. Humayun
- Subjects
Immunodiffusion ,Chromatography, Paper ,Deoxyribonucleotides ,Polynucleotides ,Cross Reactions ,medicine.disease_cause ,Tritium ,Biochemistry, Genetics and Molecular Biology (miscellaneous) ,Cross-reactivity ,Biochemistry ,Antigen-Antibody Reactions ,chemistry.chemical_compound ,Deoxyadenosine ,Antibody Specificity ,medicine ,Animals ,Nucleotide ,Bovine serum albumin ,chemistry.chemical_classification ,Antiserum ,biology ,Deoxyadenosines ,Serum Albumin, Bovine ,Ribonucleotides ,Molecular biology ,Precipitin Tests ,Adenosine Monophosphate ,chemistry ,Nucleic acid ,biology.protein ,1-Ethyl-3-(3-dimethylaminopropyl)carbodiimide ,Binding Sites, Antibody ,Rabbits ,Haptens ,DNA ,Protein Binding - Abstract
Anti-deoxyadenylate antibodies were produced in rabbits by injecting a conjugate of deoxyadenosine 5′-phosphate with bovine serum albumin. The antisera, as analyzed by double diffusion in agar and the quantitative precipitin reaction, showed hapten-specific antibodies. The specific interaction between [3H]deoxyadenylate and antiserum was studied by a sensitive nitrocellulose membrane-binding assay. The specificity of the antibodies was analyzed by measuring the effectiveness of other nucleotides or derivatives to inhibit the hapten-antibody binding. The requirements for recognition by the antibody sites were studied by using a series of naturally occurring nucleic acid components as well as some synthetic derivatives as inhibitors. The antibodies were found to show a high degree of specificity for the whole nucleotide, the base, sugar and phosphate playing almost equally important roles. There was cross reactivity with other mononucleotides, although of a low order. The antibodies were able to react with DNA and tRNA.
- Published
- 1973
31. Stochastic descriptors to study the fate and potential of naive T cell clonotypes in the periphery.
- Author
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Artalejo, J., Gómez-Corral, A., López-García, M., and Molina-París, C.
- Subjects
T cell receptors ,CLONE cells ,HOMEOSTASIS ,CELL proliferation ,STOCHASTIC analysis ,MATHEMATICAL models - Abstract
The population of naive T cells in the periphery is best described by determining both its T cell receptor diversity, or number of clonotypes, and the sizes of its clonal subsets. In this paper, we make use of a previously introduced mathematical model of naive T cell homeostasis, to study the fate and potential of naive T cell clonotypes in the periphery. This is achieved by the introduction of several new stochastic descriptors for a given naive T cell clonotype, such as its maximum clonal size, the time to reach this maximum, the number of proliferation events required to reach this maximum, the rate of contraction of the clonotype during its way to extinction, as well as the time to a given number of proliferation events. Our results show that two fates can be identified for the dynamics of the clonotype: extinction in the short-term if the clonotype experiences too hostile a peripheral environment, or establishment in the periphery in the long-term. In this second case the probability mass function for the maximum clonal size is bimodal, with one mode near one and the other mode far away from it. Our model also indicates that the fate of a recent thymic emigrant (RTE) during its journey in the periphery has a clear stochastic component, where the probability of extinction cannot be neglected, even in a friendly but competitive environment. On the other hand, a greater deterministic behaviour can be expected in the potential size of the clonotype seeded by the RTE in the long-term, once it escapes extinction. [ABSTRACT FROM AUTHOR]
- Published
- 2017
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32. Nimesulide-Induced Fixed Drug Eruption Followed by Etoricoxib-Induced Fixed Drug Eruption: An Unusual Case Report and Review of the Literature.
- Author
-
Makris, Michael, Papapostolou, Niki, Koumprentziotis, Ioannis-Alexios, Pappa, Georgia, and Katoulis, Alexander C.
- Subjects
LITERATURE reviews ,DRUG allergy ,ANTI-inflammatory agents ,BLISTERS ,PHYSICIANS ,DRUG eruptions - Abstract
Fixed drug eruption (FDE) is a well-recognized, non-immediate, drug hypersensitivity reaction, often attributed to the use of various medications, most commonly non-steroidal anti-inflammatory drugs (NSAIDs) and antibiotics. Cross-reactivity between related NSAIDs in FDE has been reported, but among chemically unrelated NSAIDs, is rare. Herein, we present a rare well-documented case where a patient initially displayed tolerance to etoricoxib after experiencing a nimesulide-induced FDE. Subsequently, the patient developed an etoricoxib-induced FDE, accompanied by the development of bullous lesions. This case report and the literature review on comparable FDE occurrences shed light on the intricate nature of FDEs, suggesting the possibility of cross-reactivity between chemically related and unrelated NSAIDs or the emergence of new drug-specific T cells without cross-reactivity after multiple exposures to a drug in a susceptible patient. Our case underscores the importance of increased awareness and vigilance among both physicians and patients in the realm of personalized medicine. Further research is needed to unravel the intricate mechanisms behind these drug eruptions, improve diagnostic approaches, and enhance patient care. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
33. Allergen Diversity and Abundance in Different Tissues of the Redclaw Crayfish (Cherax quadricarinatus).
- Author
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Jerry, Emily M., Karnaneedi, Shaymaviswanathan, Ruethers, Thimo, Jerry, Dean R., Condon, Kelly, and Lopata, Andreas L.
- Subjects
CRAYFISH ,DERMATOPHAGOIDES ,IMMUNOGLOBULIN E ,ALLERGENS ,LIQUID chromatography-mass spectrometry ,IMMUNOGLOBULINS ,CALCIUM-binding proteins ,PROTEIN binding ,CARRIER proteins - Abstract
Shellfish allergy affects ~2.5% of the global population and is a type I immune response resulting from exposure to crustacean and/or molluscan proteins. The Australian Redclaw crayfish (Cherax quadricarinatus) is a freshwater species endemic to and farmed in northern Australia and is becoming an aquaculture species of interest globally. Despite being consumed as food, allergenic proteins from redclaw have not been identified or characterised. In addition, as different body parts are often consumed, it is conceivable that redclaw tissues vary in allergenicity depending on tissue type and function. To better understand food-derived allergenicity, this study characterised allergenic proteins in various redclaw body tissues (the tail, claw, and cephalothorax) and how the stability of allergenic proteins was affected through cooking (raw vs. cooked tissues). The potential of redclaw allergens to cross-react and cause IgE-binding in patients allergic to other shellfish (i.e., shrimp) was also investigated. Raw and cooked extracts were prepared from each body part. SDS-PAGE followed by immunoblotting was performed to determine allergen-specific antibody reactivity to sarcoplasmic calcium-binding protein and hemocyanin, as well as to identify redclaw proteins binding to IgE antibodies from individual and pooled sera of shrimp-allergic patients. Liquid chromatography-mass spectrometry (LC/MS) was utilised to identify proteins and to determine the proportion within extracts. Known crustacean allergens were found in all tissues, with a variation in tissue distribution (e.g., higher levels of hemocyanin in the claw and cephalothorax than in the tail). The proportion of some allergens as a percentage of remaining heat-stable proteins increased in cooked tissues. Previously described heat-stable allergens (i.e., hemocyanin and sarcoplasmic calcium-binding protein) were found to be partially heat-labile. Immunoblotting indicated that shrimp-allergic patients cross-react to redclaw allergens. IgE-binding bands, analysed by LC/MS, identified up to 11 known shellfish allergens. The findings of this study provide fundamental knowledge into the diagnostic and therapeutic field of shellfish allergy. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
34. Cross-reactivity features of deoxynivalenol (DON)-targeted immunoaffinity columns aiming to achieve simultaneous analysis of DON and major conjugates in cereal samples.
- Author
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Gonçalves, Carlos and Stroka, Joerg
- Subjects
DEOXYNIVALENOL ,IMMUNOAFFINITY chromatography ,CEREALS as food ,MYCOTOXINS ,BINDING sites - Abstract
Immunoaffinity columns (IACs) are a well-established tool in the determination of regulated mycotoxins in food and feed commodities. However, they also have the potential to become attractive pre-concentration and clean-up materials for the determination of masked (also called modified) mycotoxins, which have been recognised as important contributors to the toxicological hazard deriving from fungal spoilage of goods. However, the information available in the literature concerning the cross-reactivity of DON-IACs against the major conjugates (DON-3-G, 15-AcDON and 3-AcDON) is incomplete and often contradictory. We have carried out a detailed characterisation of the cross-reactivity of the four main IACs brands against DON and its conjugates as well as an assessment of the competition among the analytes. Only one IAC enabled the simultaneous analysis of all relevant DON forms while two missed 15-AcDON and the fourth one missed DON-3-G and 3-AcDON. In the case of the multivalent IAC, the analytes modified at the C-3 position compete for the antibody binding with preference for 3-AcDON (less spatially hindered) while DON-3-G has the more-hindered access to the active sites. Taking into consideration the levels of DON conjugates existing in real samples, the cross-reactivity of one DON-IAC allows a quantitative analysis of all of these analytes. Important but rather neglected aspects such as the continuous supply of IACs with identical characteristics, and of columns which are strictly blank, are also addressed in this paper. [ABSTRACT FROM AUTHOR]
- Published
- 2016
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- View/download PDF
35. Remimazolam anaphylaxis in a patient not allergic to brotizolam: a case report and literature review
- Author
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Nakai, Toshihiro, Kako, Eisuke, Ota, Haruko, So, MinHye, and Sobue, Kazuya
- Published
- 2024
- Full Text
- View/download PDF
36. Pollen food allergy syndrome secondary to molds and raw mushroom cross-reactivity: a case report
- Author
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Gauld, Ryan, Walter, Graham, and Zhu, Rongbo
- Published
- 2024
- Full Text
- View/download PDF
37. Immunoglobulin E and immunoglobulin G cross-reactive allergens and epitopes between cow milk αS1-casein and soybean proteins.
- Author
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Cong, Yanjun, Li, Ye, and Li, Linfeng
- Subjects
- *
IMMUNOGLOBULIN E , *SOY proteins , *IMMUNOGLOBULIN G , *SOYMILK , *EPITOPES , *ALLERGENS - Abstract
Some infants allergic to cow milk–based formula are also sensitive to soybean-based formula. This paper aimed to explore the association of IgE and IgG cross-reactivity between α S1 -casein in cow milk (CM) and soybean proteins. The IgE and IgG cross-reactive allergens and epitopes were identified using sera from infants allergic to CM or mice monoclonal antibodies. The AA sequence alignment was performed using bioinformatics software. Finally, the digestion and heating stability of the cross-reactive allergen were explored by sodium dodecyl sulfate (SDS)-PAGE and Western blotting. The results showed that the IgE and IgG cross-reactive allergen was α subunit of β-conglycinin named Gly m Bd 60K. The IgE and IgG epitopes were the sequences at AA 319–341 and AA 164–182. No intact Gly m Bd 60K allergen could be observed after 2 min in simulated gastric fluid by SDS-PAGE. Heating did not change IgE and IgG cross-reactivity by Western blotting. Therefore, the existence of cross-reactivity between CM α S1 -casein and soybean proteins possibly contributes to the frequently observed cosensitization for these allergens in cow milk–allergic patients. The same IgE- and IgG-binding epitopes of cross-reactive allergens may provide important information for elucidation of the association between IgG and IgE antibody generation. [ABSTRACT FROM AUTHOR]
- Published
- 2020
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38. Does Lidocaine Cause False Positive Results on Cocaine Urine Drug Screen?
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Kim, Eungjae, Murray, Brian Patrick, Salehi, Maryam, Moran, Tim P., Carpenter, Joseph E., Koch, David D., Ritchie, James C., Schindler, Joanna M., and Morgan, Brent W.
- Published
- 2019
- Full Text
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39. Sensitisation to lipid transfer proteins in pollen - allergic adults with food allergy.
- Author
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Wawrzeńczyk, Adam, Żbikowska-Gotz, Magdalena, Wawrzeńczyk, Anna, and Bartuzi, Zbigniew
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LIPID transfer protein ,ALLERGIC rhinitis ,FOOD allergy ,RESPIRATORY therapy ,IMMUNOGLOBULIN E ,ANTIGENS - Abstract
Introduction: Food allergy affects 6-17% of Europeans and in 60% of the cases it is accompanied by inhalation allergy. This is due to two phenomena, namely co-sensitisation and cross-reactivity, which are caused by panallergens, such as lipid transfer proteins (LTP). These proteins are classified as defence proteins PR-14 and are responsible for protecting the plant from bacterial and fungal infections. Clinically, LTP allergy manifests with severe anaphylactic reactions or milder reactions, such as oral allergy syndrome. Aim: To assess the incidence of specific IgE to components belonging to LTP based on molecular testing. Material and methods: The study was conducted at the Department of Allergy, Clinical Immunology and Internal Diseases in Bydgoszcz, Poland. A total of 50 subjects participated in the study. Each subject had their asIgE levels determined with ImmunoCap ISAC. Results and Conclusions: Antigen-specific IgE antibodies to LTP components were identified in 24% of the subjects. In subjects with LTP allergy, adverse skin reactions were the most common after consumption of sensitising foods. Peach was the most common food allergen triggering these reactions. The Artemisia vulgaris component Art v 3 was the precursor of LTP allergy in our study population: the highest incidence and the highest mean levels of asIgE were demonstrated for this component. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
40. Allergic reactions to genus Morus plants: a review.
- Author
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Papia, F., Incorvaia, C., Genovese, L., Gangemi, S., and Minciullo, P. L.
- Subjects
ANAPHYLAXIS ,BERRIES ,FOOD allergy ,RESPIRATORY allergy ,URTICARIA ,DISEASE risk factors - Abstract
Mulberry is a plant belonging to the family Moraceae, and genus Morus. Allergic sensitization to mulberries has been reported as both food allergy or respiratory allergy, and cross-reactivity between mulberries and other pollens or fruits was described. Clinically, in the articles reporting mulberry allergy, the reactions included respiratory allergy, airborne contact urticaria, anaphylaxis, oral allergy syndrome, and food induced urticaria. As far as cross-reactivity is concerned, the allergens identified thus far in mulberries include pathogenesis-related (PR)10 proteins, with sequence identity to Bet v 1 from birch, lipid transfer (LTP)1 proteins with identity with LTPs from Rosaceae family plants, panallergens groups, and also ubiquitin-like protein and cystatin-like protein. The two latter proteins account for cross-reactions with Parietaria judaica and Olea europaea. Such large cross-reactivity warrants to pay particular attention to the risk of systemic reactions to foods, particularly in subjects sensitized to birch, parietaria or olive pollens. In fact, the increasing use of mulberry as a food product, which is encouraged by its remarkable antioxidant power, expose sensitized patients to possible reactions after ingesting foods, dietary supplements or nutraceuticals containing mulberry. Mulberry allergenicity can vary according to the processing methods used since some allergens are thermostable and other loss their reactivity during heating. [ABSTRACT FROM AUTHOR]
- Published
- 2020
- Full Text
- View/download PDF
41. T cells specific to multiple Bet v 1 peptides are highly cross-reactive toward the corresponding peptides from the homologous group of tree pollens.
- Author
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Lund, Gitte, Christensen, Lars Harder, Ihlemann, Jacob, Andersen, Peter Sejer, Wambre, Erik, Würtzen, Peter Adler, and Gupta, Shashank
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IMMUNOGLOBULIN E ,MONONUCLEAR leukocytes ,T cells ,PEARSON correlation (Statistics) ,POLLEN ,PEPTIDES - Abstract
Background: Allergens from Fagales trees frequently cause spring allergy in Europe, North America, and some parts of Asia. The definition of the birch homologous group, which includes birch (Bet v), oak (Que a), alder (Aln g), hazel (Cor a), hornbeam (Car b), beech (Fag s), and chestnut (Cas s), is based on high allergen sequence identity and extensive IgE cross-reactivity. Clinical effect was seen during the alder/hazel, birch, and oak pollen seasons after treatment with tree SLIT-tablets containing only birch allergen extract. Here, we characterize Tcell reactivity with respect to epitope specificities and cross-reactivity toward various Bet v 1 family members, (PR-10/group 1 major allergens). This crossreactivity may be part of the immunological basis of clinical effect or crossprotection when exposed to birch homologous tree species. Method: T-cell lines were generated from 29 birch-allergic individuals through stimulation of peripheral blood mononuclear cells (PBMCs) with birch/Bet v or oak/Que a allergen extracts. T-cell responses to allergen extracts, purified group 1 allergens, and overlapping 20-mer peptides (Bet v 1, Aln g 1, Cor a 1, and Que a 1) were investigated by T-cell proliferation and cytokine production. Crossreactivity was evaluated based on Pearson’s correlations of response strength and further investigated by flow cytometry using tetramer staining for homologous peptide pairs. Results: T-cell reactivity toward extracts and group 1 allergens from across the birch homologous group was observed for birch/Bet v as well as oak/Que a Tcell lines. T-cell lines responded to multiple Bet v 1 homologous peptides from Aln g 1 and Cor a 1 and a subset of Que a 1 peptides. Significant Pearson’s correlations between frequently recognized peptides derived from Bet v 1 and the corresponding peptides derived from alder, hazel, and oak strongly supported the T-cell cross-reactivity toward these allergens. Cross-reactivity between birch and birch homologous peptides was confirmed by pMHCII tetramer staining. Conclusion: T cells from birch tree pollen allergic individuals respond to multiple trees within the birch homologous group in accordance with the level of sequence homology between Bet v 1 family members, (PR-10 allergens) from these allergen sources, confirming the basis for clinical cross-protection. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
42. Antibody Cross-Reactivity in Serodiagnosis of Lyme Disease.
- Author
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Grąźlewska, Weronika and Holec-Gąsior, Lucyna
- Subjects
LYME disease ,RELAPSING fever ,TICK-borne diseases ,SERODIAGNOSIS ,BORRELIA burgdorferi ,TREPONEMA pallidum - Abstract
Lyme disease is a tick-borne disease caused by spirochetes belonging to the Borrelia burgdorferi sensu lato complex. The disease is characterized by a varied course; therefore, the basis for diagnosis is laboratory methods. Currently, a two-tiered serological test is recommended, using an ELISA as a screening test and a Western blot as a confirmatory test. This approach was introduced due to the relatively high number of false-positive results obtained when using an ELISA alone. However, even this approach has not entirely solved the problem of false-positive results caused by cross-reactive antibodies. Many highly immunogenic B. burgdorferi s.l. proteins are recognized nonspecifically by antibodies directed against other pathogens. This also applies to antigens, such as OspC, BmpA, VlsE, and FlaB, i.e., those commonly used in serodiagnostic assays. Cross-reactions can be caused by both bacterial (relapsing fever Borrelia, Treponema pallidum) and viral (Epstein–Baar virus, Cytomegalovirus) infections. Additionally, a rheumatoid factor has also been shown to nonspecifically recognize B. burgdorferi s.l. proteins, resulting in false-positive results. Therefore, it is necessary to carefully interpret the results of serodiagnostic tests so as to avoid overdiagnosis of Lyme disease, which causes unnecessary implementations of strong antibiotic therapies and delays in the correct diagnosis. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
43. Structural Similarities, in Relation with the Cross-Reactivity, of Hymenoptera Allergenic Dipeptidyl Peptidases IV—An Overall Comparison Including a New Dipeptidyl Peptidase IV Sequence from Vespa velutina.
- Author
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Monsalve, Rafael I., Lombardero, Manuel, Christensen, Lars H., Núñez-Acevedo, Beatriz, González-de-Olano, David, Sobrino-García, Miriam, Castillo-Loja, Rosita M., Bravo, Susana B., Alonso-Sampedro, Manuela, and Vidal, Carmen
- Subjects
CD26 antigen ,PEPTIDASE ,HYMENOPTERA ,CROSS reactions (Immunology) ,HOMOLOGY (Biology) - Abstract
(1) Background: Dipeptidyl Peptidases IV (DPPIVs), present in many organisms, are minor components in the venoms of Hymenoptera, where they have been identified as cross-reactive allergenic molecules. Considering that the structure of homologous DPPIVs is well characterized, we aimed to explain which regions have higher similarity among these proteins and present a comparison among them, including a new Vespa velutina DPPIV sequence. Moreover, two cases of sensitization to DPPIVs in wasp- and honeybee-sensitized patients are presented. (2) Methods: Proteomic analyses have been performed on the venom of the Asian hornet Vespa velutina to demonstrate the sequence of its DPPIV (allergen named Vesp v 3, with sequence accession number P0DRB8, and with the proteomic data available via ProteomeXchange with the identifier PXD046030). A comparison performed through their alignments and analysis of the three-dimensional structure showed a region with higher similarity among Hymenoptera DPPIVs. Additionally, ImmunoCAP™ determinations (including specific inhibition experiments), as well as IgE immunoblotting, are performed to demonstrate the allergenicity of Api m 5 and Ves v 3. (3) Results and Conclusions: The data presented demonstrate that the similarities among Hymenoptera DPPIVs are most likely localized at the C-terminal region of these enzymes. In addition, a higher similarity of the Vespa/Vespula DPPIVs is shown. The clinical cases analyzed demonstrated the allergenicity of Api m 5 and Ves v 3 in the sera of the allergic patients, as well as the presence of this minor component in the preparations used in venom immunotherapy. [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
44. Immunodiagnosis of sheep infections with Echinococcus granulosus: in 35 years where have we come?
- Author
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M cManus, D. P.
- Subjects
IMMUNODIAGNOSIS ,SHEEP as laboratory animals ,ECHINOCOCCUS granulosus ,IMMUNOLOGY ,ELECTROPHORESIS ,IMMUNOGLOBULINS ,DRUG development - Abstract
W. K. Yong and D. D. Heath published in 1979 a seminal paper in the first issue of Parasite Immunology describing their efforts to determine whether the arc 5 precipitin band, formed when test human serum is reacted against electrophoresed hydatid cyst fluid antigen, would be a suitable immunodiagnostic test for the identification of sheep infected with Echinococcus granulosus. Although they found antibodies to arc 5 in the sera of hydatid-infected sheep, the sera of some sheep harbouring Taenia ovis and T. hydatigena also precipitated the hydatid cyst fluid arc 5 antigen, so they concluded arc 5 antibodies were not suitable for the specific immunodiagnosis of E. granulosus infection in sheep in New Zealand. Subsequent work has shown that the existence of multiple infections with different taeniid species, antigenic cross-reactivity between these related parasites and the low level of specific antibody response to infection continue to hinder efforts to improve the diagnosis of hydatid infection in sheep and other natural intermediate hosts, thereby preventing the development of any practical test. In particular, the poor antibody response of ruminants to naturally acquired hydatid infection may prove an insurmountable barrier in future efforts to develop a reliable and accurate immunological test. [ABSTRACT FROM AUTHOR]
- Published
- 2014
- Full Text
- View/download PDF
45. Development of flavivirus subviral particles with low cross-reactivity by mutations of a distinct antigenic domain
- Author
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Tabata, Koshiro, Itakura, Yukari, Ariizumi, Takuma, Igarashi, Manabu, Kobayashi, Hiroko, Intaruck, Kittiya, Kishimoto, Mai, Kobayashi, Shintaro, Hall, William W., Sasaki, Michihito, Sawa, Hirofumi, and Orba, Yasuko
- Published
- 2023
- Full Text
- View/download PDF
46. Infection and Vaccine Induced Spike Antibody Responses Against SARS-CoV-2 Variants of Concern in COVID-19-Naïve Children and Adults
- Author
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Pillay, Aleha, Yeola, Avani, Tea, Fiona, Denkova, Martina, Houston, Samuel, Burrell, Rebecca, Merheb, Vera, Lee, Fiona X. Z., Lopez, Joseph A., Moran, Lilly, Jadhav, Ajay, Sterling, Katrina, Lai, Catherine L., Vitagliano, Tennille L., Aggarwal, Anupriya, Catchpoole, Dan, Wood, Nicholas, Phan, Tri Giang, Nanan, Ralph, Hsu, Peter, Turville, Stuart G., Britton, Philip N., and Brilot, Fabienne
- Published
- 2023
- Full Text
- View/download PDF
47. Global analysis of T-cell groups reveals immunological features and common antigen targets of digestive tract tumors
- Author
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Li, Xiaoxue, Zhang, Yuchao, Guo, Shiwei, Wu, Zhenchuan, Wang, Hailong, Huang, Yi, Wang, Yue, Qiu, Mengni, Lang, Jingyu, Xiao, Yichuan, Zhu, Yufei, Jin, Gang, Hu, Landian, and Kong, Xiangyin
- Published
- 2024
- Full Text
- View/download PDF
48. Short communication: Mycolicibacterium smegmatis, basonym Mycobacterium smegmatis, causing pyogranulomatous mastitis and its cross-reactivity in bovine (para)tuberculosis testing.
- Author
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Supré, K., Roupie, V., Ribbens, S., Stevens, M., Boyen, F., and Roels, S.
- Subjects
- *
MYCOBACTERIUM avium paratuberculosis , *MYCOBACTERIUM bovis , *MASTITIS , *MYCOBACTERIUM smegmatis , *BOVINE mastitis - Abstract
Different mycobacterial species are encountered in bovine medicine. The fastidiously growing mycobacteria (Mycobacterium bovis as the cause of bovine tuberculosis, and Mycobacterium avium ssp. paratuberculosis , MAP, as the cause of paratuberculosis) are well known and targeted in eradication/control or monitoring programs in different countries, whereas the rapidly growing species is only rarely identified from bovine disease. The latter have occasionally been reported as the cause of bovine clinical mastitis, but recent reports are scarce. In this study, Mycolicibacterium smegmatis (basonym Mycobacterium smegmatis) was identified as cause of granulomatous, relapsing clinical mastitis in 2 cows from one Belgian dairy herd. Milk, blood, and fecal samples were collected, as well as tissue samples after the cows were culled. Serological analysis conducted on milk and serum samples resulted in positive reactions for MAP, but negative for Mycobacterium bovis. Production of IFN-γ showed sensitization with mycobacteria or similar organisms, other than M. bovis , in one cow. Detection of MAP by bacteriological culture and IS 900 -based quantitative PCR on milk and feces remained negative. In conclusion, this paper describes M. smegmatis as a cause of bovine clinical mastitis in Belgium and suggests cross-reactivity of the intramammary M. smegmatis infection with routinely used serological tests for MAP. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
49. Analytical barriers in clinical B-type natriuretic peptide measurement and the promising analytical methods based on mass spectrometry technology.
- Author
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Xiao, Peng, Li, Hongmei, Li, Xianjiang, and Song, Dewei
- Subjects
NATRIURETIC peptides ,MASS spectrometry ,BIOLOGICAL tags ,AMINO acids ,IMMUNOASSAY - Abstract
B-type natriuretic peptide (BNP) is a circulating biomarker that is mainly applied in heart failure (HF) diagnosis and to monitor disease progression. Because some identical amino acid sequences occur in the precursor and metabolites of BNP, undesirable cross-reactions are common in immunoassays. This review first summarizes current analytical methods, such as immunoassay- and mass spectrometry (MS)-based approaches, including the accuracy of measurement and the inconsistency of the results. Second, the review presents some promising approaches to resolve the current barriers in clinical BNP measurement, such as how to decrease cross-reactions and increase the measurement consistency. Specific approaches include research on novel BNP assays with higher-specificity chemical antibodies, the development of International System of Units (SI)-traceable reference materials, and the development of structure characterization methods based on state-of-the-art ambient and ion mobility MS technologies. The factors that could affect MS analysis are also discussed, such as biological sample cleanup and peptide ionization efficiency. The purpose of this review is to explore and identify the main problems in BNP clinical measurement and to present three types of approaches to resolve these problems, namely, materials, methods and instruments. Although novel approaches are proposed here, in practice, it is worth noting that the BNP-related peptides including unprocessed proBNP were all measured in clinical BNP assays. Therefore, approaches that aimed to measure a specific BNP or proBNP might be an effective way for the standardization of a particular BNP form measurement, instead of the standardization of "total" immunoreactive BNP assays in clinical at present. [ABSTRACT FROM AUTHOR]
- Published
- 2019
- Full Text
- View/download PDF
50. Allergenicity of Soybean: New Developments in Identification of Allergenic Proteins, Cross-Reactivities and Hypoallergenization Technologies.
- Author
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L'Hocine, Lamia and Boye, Joyce I.
- Subjects
SOYBEAN ,FOOD allergy ,ALLERGENS ,PROTEINS ,FOOD research - Abstract
Soybean is considered one of the "big eight" foods that are believed to be responsible for 90% of all allergenic reactions. Soy allergy is of particular importance, because soybeans are widely used in processed foods and, therefore, represent a particularly insidious source of hidden allergens. Although significant advances have been made in the identification and characterization of soybean allergens, scientists are not completely certain about which proteins in soy cause allergic reactions. At least 16 allergens have been identified. Most of them, as with other plant food allergens, have a metabolic, storage, or protective function. These allergens belong to protein families which have conserved structural features in relation with their biological activity, which explains the wide immunochemical cross-recognition observed among members of the legume family. Detailed analysis of the structure-allergenicity relationships has been hampered by the complexity and heterogeneity of soybean proteins. A variety of technological approaches have been attempted to decrease soybean allergenicity. This paper provides a comprehensive review of the current body of knowledge on the identification and characterization of soybean allergens, as well as an update on current hypoallergenization techniques. [ABSTRACT FROM AUTHOR]
- Published
- 2007
- Full Text
- View/download PDF
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