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A simple and rapid immunochromatographic test kit for rabies diagnosis
- Source :
- Microbiology and Immunology
- Publication Year :
- 2008
- Publisher :
- Blackwell Publishing Asia, 2008.
-
Abstract
- In rabies endemic countries, funds and infrastructure are often insufficient to employ the approved gold standard for the definitive diagnosis of rabies: the direct fluorescent test. In the present study, two types (type 1 and 2) of an ICT kit were evaluated for detection of rabies. These were developed using monoclonal antibodies which recognize epitope II and III of the nucleoprotein of rabies virus. Both kits specifically detected all rabies virus strains and there was no cross reactivity with Lyssaviruses (Lagos, Mokola and Duvenhage), Rhabdovirus (VSV and Oita 296/1972) and other common canineāpathogenic viruses. In type 1, a single type of monoclonal antibody was used. It was capable of detecting recombinant nucleoprotein and showed sensitivity of 95.5% (42/44) and specificity of 88.9% (32/36) using brain samples from rabid dogs. In contrast, type 2 which was made of two different monoclonal antibodies had a lower sensitivity of 93.2% (41/44) and higher specificity of 100% (36/36). These ICT kits provide a simple and rapid method for rabies detection. They need neither cold chain for transportation nor complicated training for personnel. This diagnostic test is suitable for rabies screening, particularly in areas with a high prevalence of rabies and where the fluorescent antibody test is not available.
- Subjects :
- medicine.drug_class
Rabies
Immunology
Biology
medicine.disease_cause
Monoclonal antibody
Antibodies, Viral
Microbiology
Cross-reactivity
Immunoenzyme Techniques
Dogs
Virology
medicine
Animals
Direct fluorescent antibody
Antigens, Viral
rapid diagnosis
Editor Communicated Paper
immunochromatographic test
Rabies virus
Antibodies, Monoclonal
Gold standard (test)
medicine.disease
Nucleoprotein
Nucleoproteins
biology.protein
Reagent Kits, Diagnostic
Antibody
Subjects
Details
- Language :
- English
- ISSN :
- 13480421 and 03855600
- Volume :
- 52
- Issue :
- 4
- Database :
- OpenAIRE
- Journal :
- Microbiology and Immunology
- Accession number :
- edsair.doi.dedup.....177754a2f0928ec63f0f4bc5f6345fb6