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Voltammetric-based immunosensor for the detection of SARS-CoV-2 nucleocapsid antigen
- Source :
- Mikrochimica Acta
- Publication Year :
- 2021
- Publisher :
- Springer Vienna, 2021.
-
Abstract
- Since the COVID-19 disease caused by the novel severe acute respiratory syndrome coronavirus 2 (SARS- CoV-2) was declared a pandemic, it has spread rapidly, causing one of the most serious outbreaks in the last century. Reliable and rapid diagnostic tests for COVID-19 are crucial to control and manage the outbreak. Here, a label-free square wave voltammetry-based biosensing platform for the detection of SARS-CoV-2 in nasopharyngeal samples is reported. The sensor was constructed on screen-printed carbon electrodes coated with gold nanoparticles. The electrodes were functionalized using 11-mercaptoundecanoic acid (MUA) which was used for the immobilization of an antibody against SARS-CoV-2 nucleocapsid protein (N protein). The binding of the immunosensor with the N protein caused a change in the electrochemical signal. The detection was realised by measuring the change in reduction peak current of a redox couple using square wave voltammetry at 0.04 V versus Ag ref. electrode on the immunosensor upon binding with the N protein. The electrochemical immunosensor showed high sensitivity with a linear range from 1.0 pg.mL−1 to 100 ng.mL−1 and a limit of detection of 0.4 pg.mL−1 for the N protein in PBS buffer pH 7.4. Moreover, the immunosensor did not exhibit significant response with other viruses such as HCoV, MERS-CoV, Flu A and Flu B, indicating the high selectivity of the sensor for SARS-CoV-2. However, cross reactivity of the biosensor with SARS-CoV is indicated, which gives ability of the sensor to detect both SARS-CoV and SARS-CoV-2. The biosensor was successfully applied to detect the SARS-CoV-2 virus in clinical samples showing good correlation between the biosensor response and the RT-PCR cycle threshold values. We believe that the capability of miniaturization, low-cost and fast response of the proposed label-free electrochemical immunosensor will facilitate the point-of-care diagnosis of COVID 19 and help prevent further spread of infection.
- Subjects :
- viruses
Metal Nanoparticles
02 engineering and technology
Biosensing Techniques
Immunosensor
medicine.disease_cause
01 natural sciences
Cross-reactivity
Analytical Chemistry
COVID-19 Testing
Antigen
Limit of Detection
Nasopharynx
medicine
Electrochemical detection
Coronavirus Nucleocapsid Proteins
Humans
Sulfhydryl Compounds
Voltammetry
Electrodes
Antibody
Detection limit
Nucleocapsid protein
Immunoassay
Original Paper
Chromatography
medicine.diagnostic_test
Chemistry
SARS-CoV-2
010401 analytical chemistry
Fatty Acids
virus diseases
COVID-19
Electrochemical Techniques
021001 nanoscience & nanotechnology
Phosphoproteins
Carbon
0104 chemical sciences
Linear range
Colloidal gold
Gold
0210 nano-technology
Biosensor
Antibodies, Immobilized
Subjects
Details
- Language :
- English
- ISSN :
- 14365073 and 00263672
- Volume :
- 188
- Issue :
- 6
- Database :
- OpenAIRE
- Journal :
- Mikrochimica Acta
- Accession number :
- edsair.doi.dedup.....a7f381f38695c2844ef003988c025874