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Influence of spironolactone on neonatal screening for congenital adrenal hyperplasia
- Publication Year :
- 1999
- Publisher :
- BMJ Group, 1999.
-
Abstract
- AIM To determine if the diuretic spironolactone cross reacts with 17α-hydroxyprogesterone (17OHP) in an enzyme linked immunosorbent assay (ELISA) kit used for the mass screening of congenital adrenal hyperplasia. METHODS Concentrations of 17OHP on a blood filter paper disc were measured using an ELISA kit (kit C-7: ENZAPLATE N-17α -OHP-7; Chiron, Tokyo, Japan). The cross reactivity of spironolactone and its metabolites with 17OHP was determined. The concentrations of spironolactone and its metabolites in blood were measured using HPLC (high performance liquid chromatography). RESULTS Spironolactone cross reacted with 17OHP using kit C-7 (0.01%), by increasing 17OHP concentration in a dose dependent manner. The blood concentration of spironolactone and its metabolites was nearly 900 ng/ml, high enough to show an additive effect on the 17OHP concentration. About 12% of the false positive cases screened using the kit were due to the administration of spironolactone. CONCLUSIONS Spironolactone interferes with 17OHP concentrations, leading to false positive test results for CAH.
- Subjects :
- Male
medicine.medical_specialty
medicine.medical_treatment
Dose dependence
Enzyme-Linked Immunosorbent Assay
Spironolactone
medicine.disease_cause
High-performance liquid chromatography
Cross-reactivity
chemistry.chemical_compound
Neonatal Screening
Internal medicine
Medicine
Humans
Congenital adrenal hyperplasia
False Positive Reactions
Diuretics
Mass screening
Adrenal Hyperplasia, Congenital
business.industry
17-alpha-Hydroxyprogesterone
Infant, Newborn
Obstetrics and Gynecology
General Medicine
Original Articles
medicine.disease
Endocrinology
chemistry
Blood filter paper
Pediatrics, Perinatology and Child Health
Diuretic
business
Subjects
Details
- Language :
- English
- Database :
- OpenAIRE
- Accession number :
- edsair.doi.dedup.....62dec771f3dddf594fb976fc897396a3