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Single dose vaccination of the ASO3-adjuvanted A(H1N1)pdm09 monovalent vaccine in health care workers elicits homologous and cross-reactive cellular and humoral responses to H1N1 strains
- Source :
- Human Vaccines & Immunotherapeutics
- Publication Year :
- 2015
- Publisher :
- Taylor & Francis, 2015.
-
Abstract
- Healthcare workers (HCW) were prioritized for vaccination during the 2009 influenza A(H1N1)pdm09 pandemic. We conducted a clinical trial in October 2009 where 237 HCWs were immunized with a AS03-adjuvanted A(H1N1)pdm09 monovalent vaccine. In the current study, we analyzed the homologous and cross-reactive H1N1 humoral responses using prototype vaccine strains dating back to 1977 by the haemagglutinin inhibition (HI), single radial hemolysis SRH), antibody secreting cell (ASC) and memory B cell (MBC) assays. The cellular responses were assessed by interferon-γ (IFN-γ) ELISPOT and by intracellular staining (ICS) for the Th1 cytokines IFN-γ, interleukin-2 (IL-2) and tumor necrosis factor-α (TNF-α). All assays were performed using blood samples obtained prior to (day 0) and 7, 14 and 21 d post-pandemic vaccination, except for ASC (day 7) and ICS (days 0 and 21). Vaccination elicited rapid HI, SRH and ASC responses against A(H1N1)pdm09 which cross reacted with seasonal H1N1 strains. MBC responses were detected against the homologous and seasonal H1N1 strains before vaccination and were boosted 2 weeks post-vaccination. An increase in cellular responses as determined by IFN-γ ELISPOT and ICS were observed 1–3 weeks after vaccination. Collectively, our data show that the AS03-adjuvanted A(H1N1)pdm09 vaccine induced rapid cellular and humoral responses against the vaccine strain and the response cross-reacted against prototype H1N1 strains dating back to 1977. publishedVersion
- Subjects :
- Male
Hemagglutination Inhibition Tests
pandemic influenza
Time Factors
cross-reactivity
alpha-Tocopherol
Polysorbates
medicine.disease_cause
Antibodies, Viral
Cross-reactivity
AS03
0302 clinical medicine
Influenza A Virus, H1N1 Subtype
Immunologic
Influenza A Virus
Immunology and Allergy
Interferon gamma
Viral
030212 general & internal medicine
Memory B cell
0303 health sciences
Immunity, Cellular
ELISPOT
H1N1
Humoral
virus diseases
Middle Aged
3. Good health
Vaccination
Drug Combinations
Influenza Vaccines
HCW
Tumor necrosis factor alpha
Female
Human
medicine.drug
Research Paper
Adult
Squalene
Health Personnel
Immunology
Cross Reactions
Antibodies
03 medical and health sciences
Interferon-gamma
Adjuvants, Immunologic
Influenza, Human
medicine
Humans
H1N1 Subtype
Adjuvants
Immunization Schedule
030304 developmental biology
Pharmacology
business.industry
healthcare workers
Immunity
Healthcare workers
Immune responses
Pandemic influenza
Immunity, Humoral
vaccination
Virology
Influenza
immune responses
Cellular
business
Subjects
Details
- Language :
- English
- ISSN :
- 2164554X and 21645515
- Volume :
- 11
- Issue :
- 7
- Database :
- OpenAIRE
- Journal :
- Human Vaccines & Immunotherapeutics
- Accession number :
- edsair.doi.dedup.....a4879d0775353dbee57921a2ce5c28bd