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Objective: To explore the effect of vitamin D adjuvant therapy in children with allergic asthma and its effects on intestinal flora and immune function. Methods: 120 children with allergic asthma in our hospital from January 2021 to December 2023 were divided into control group and combination group with oral vitamin D3 in the control group, and both groups were treated for 6 months. Compare the two groups of children. Results: After treatment, the symptoms and medication points of the two groups were reduced, the immune function index was improved, the intestinal flora was improved, and the combination group was better than the con- trol group (P<0.05), and the incidence of adverse reactions in the combination group was no different than that in the control group (P>0.05). Conclusion: The adjuvant therapy with vitamin D can significantly improve the clinical effects of allergic asthma in children, which may be related to the improvement of the immune function and intestinal flora of the children. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of dobutamine combined with anisodamine hydrobromide on endothelial function and immune function in patients with septic shock. A total of 100 patients with septic shock admitted from January 2023 to January 2024 were randomly divided into control group and observation group, with 50 cases each. The control group was given dobutamine and normal saline, and the observation group was given dobutamine and anisodaminehydrobromide. After treatment, compared with the observation group, the levels of TNF-α, CRP, IL-6, PCT, VO2, O2ER, E-SLT, sTM, ET-1, Ang-2 and CD8+ in the control group were all increased, while the levels of ScvO2, DO2, CD4+, CD3+ and CD4+/CD8+ were decreased. There was statistical difference (P<0.05). In addition, the length of hospital stay, time of getting out of bed and duration of shock in control group were significantly higher than those in observation group, while the total clinical effective rate was lower than that in observation group (P<0.05). Statistical difference between the two groups (P>0.05). Dobutamine combined with anisodamine hydrobromide can improve the endothelial function, clear the inflammatory mediators, improve immune function, and reduce the incidence of complications on septic shock patients, which has clinical application value. [ABSTRACT FROM AUTHOR]

Objective: To explore the effect of electronic bronchoscope pulmonary alveolar lavage and splenic polypeptide on the treatment effect, immune function and respiratory dynamics indicators in severe lung infection. Methods: 88 cases of severe lung infection admitted to our hospital from January 2021 to December 2023 were divided into observation group and matched group, with 44 cases in each group. They were divided into an observation group and a matched group, with 44 cases in each group. All patients received routine treatments such as nutritional support, spasmolysis, oxygen therapy, phlegm reduction, mechanical ventilation, and anti infection. The matched group received electronic bronchoscopy bronchoalveolar lavage, while the observation group received an increase in spleen peptides on the basis of the matched group. Compare its clinical efficacy, changes in inflammatory factors, respiratory dynamics indicators, and immune function before and Post-treatment. Results: Compared with the matched group, the observation group had a higher total effective rate of treatment (P<0.05); Post-treatment, the levels of inflammatory factors in both groups of patients decreased, and compared with the matched group, the observation group was lower (P<0.05); Post-treatment, the respiratory mechanics indicators of both groups of patients decreased, and compared with the matched group, the observation group was lower (P<0.05); Post-treatment, the T cell subsets in both groups increased, and compared with the matched group, the observation group had a higher level (P<0.05). Conclusion: The combination of electronic bronchoscopy alveolar lavage and splenic peptide can improve the clinical efficacy of severe pulmonary infection, reduce the level of inflammatory factors in the body, improve respiratory dynamics, and enhance immune function. [ABSTRACT FROM AUTHOR]

Objective: To observe the effects of sevoflurane combine with remifentanil on awakening quality, stress response and cellular immune function in children with indirect inguinal hernia undergoing laparoscopic operation. Methods: 102 children with indirect inguinal hernia received in our hospital from November 2023 to January 2024 were divided into control group (51 cases, anesthesia regimen was remifentanil) and observation group (51 cases, anesthesia regimen was Propofol combined with remifentanil) by random number table method. The hemodynamic indexes [heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP)], Awakening situation [time of awakening, extubation and respiratory recovery], stress response indexes (blood glucose and plasma cortisol) and cellular immune function (CD4", CD8", CD4/CD8") were compared in two groups, the incidence of adverse reactions was statistically compared in two groups. Results: HR, SBP and DBP at 10 min after skin incision and extubation in observation group were higher than those in control group (P<0.05). The time of awakening, extubation and respiratory recovery in observation group were shorter than those in control group (P<0.05). The blood glucose and cortisol in observation group were lower than those in control group at the time of skin incision and extubation (P<0.05). 12 h after operation, CD4 and CD4/CD8+ in observation group were higher than those in control group, and CD8 was lower than that in control group (P<0.05). The total incidence of adverse reactions: control group was 9.80%; observation group was 11.76%, there was no difference in two groups (P>0.05). Conclusion: Remifentanil and sevoflurane are selected as anesthetic drugs for children with indirect inguinal hernia undergoing laparoscopic operation, which can maintain hemodynamic stability, improve awakening quality, reduce stress response and relieve immunosuppression. [ABSTRACT FROM AUTHOR]

Objective: To study the relationship between high-resolution CT (HRCT) typing and airway inflammation, immune function and clinical efficacy in patients with chronic obstructive pulmonary disease (COPD). Methods: 165 COPD patients admitted to the Affiliated Hospital of Traditional Chinese Medicine of Xinjiang Medical University from May 2020 to August 2023 were selected, and patients were divided into type A group (n=81), type E group (n=46) and type M group (n=38) according to HRCT imaging phenotype. Pulmonary function indexes[forced expiratory volume in the first second (FEV1), percentage of forced expiratory volume in the first second to predicted value (FEV1%), forced expiratory volume in the first second/forced vital capacity (FEV1/FVC), residual volume/total lung capacity (RV/TLC) ], airway inflammation indexes[C-reactive protein (CRP), interleukin-6 (IL-6) ], immune function indexes and clinical efficacy were compared in each group. Results: FEV1, FEV1% and FEV1/FVC in type E group and M group were lower than those in type A group, and type M group were lower than those of type E group (P＜0. 05). RV/TLC in type E group and type M group was higher than that in type A group, and RV/TLC in type M group was higher than that in type E group (P＜0. 05). The levels of CRP and IL-6 in type A group and type E group were lower than those in type M group, and type E group were lower than those of type A group (P＜0. 05). CD3+ and CD4+/CD8+ in type E group and type M group were lower than those in type A group, and type M group were lower than those of type E group (P＜0. 05). The total effective rate in group A was higher than that in type E group and type M group, and type E group was higher than that of type M group (P＜0. 05). Conclusion: There are great differences in airway inflammation, immune function and clinical efficacy among COPD patients with different HRCT types, this situation can be paid attention to in the process of diagnosis and treatment, so as to provide support for clinical diagnosis and treatment. [ABSTRACT FROM AUTHOR]

Objective: To explore the effect of the combination of activating blood circulation and removing stasis with thymic method based on Yishen Huayu Tang on renal interstitial damage after chemotherapy in advanced cervical cancer and its immunomodulatory effect. Method: 80 patients with renal interstitial damage after chemotherapy for advanced cervical cancer admitted to our hospital from October 2020 to October 2023 were selected. They were divided into an observation group and a control group, with40 cases. All patients were treated with platinum containing chemotherapy, while the control group was treated with thymic method. The observation group was treated with Yishen Huayu Tang combined with thymic method. The changes in renal function indicators and immune function before and Post-treatment were compared between the two groups, and the incidence of chemotherapy adverse reactions was compared between the two groups, Finally, the quality of life of the two groups of patients was evaluated using the WHO QOL-100 before and Post-treatment. Result: Post-treatment, the levels of SCr, BUN, β2-MG, and Cys C were decreased in the observation group was lower than that in the control group (P＜0.05); Post-treatment, the CD4+, CD3+, and CD4+/CD8+values, Ig M, Ig G,and Ig A levels of the two groups of patients decreased, and the observation group was higher than the control group (P＜0.05); The incidence of chemotherapy adverse reactions related to heart function impairment, liver function impairment, fever, gastrointestinal reactions, decreased hemoglobin, thrombocytopenia, and bone marrow suppression in the observation group was lower than that in the control group（P＜0.05); Post-treatment, the WHOQOL-100 related scores of both groups of patients increased, and the observation group was higher than the control group（P＜0.05). Conclusion: The combination of Huoxue Huayu and thymus therapy based on Yishen Huayu Tang can improve the renal function of patients with renal interstitial damage after chemotherapy for advanced cervical cancer, and can produce immune regulatory effects, improve patient immune function, reduce the incidence of other chemotherapy adverse reactions, and enhance patient quality of life. [ABSTRACT FROM AUTHOR]

Objective: To observe the combined treatment of jiawei shaogan fuzi decoction and mild moxibustion in patients with active rheumatoid arthritis (RA) of cold-dampness obstruction type, the effects on immune function and serum interleukin (IL)-1β, IL-6, IL-18. Methods: 142 patients with active RA (cold-dampness obstruction type) were divided into control group (mild moxibustion treatment, n=71) and combine group (Jiawei Shaogan Fuzi Decoction combine with mild moxibustion treatment, n=71) according to the ran- dom number table method. The efficacy, laboratory indexes, immune function and serum inflammatory factors were compared between two groups. Results: Compared with control group, the total clinical effective rate in combine group was significantly higher (P<0.05). Compared with control group after treatment, rheumatoid factor (RF), erythrocyte sedimentation rate(ESR), C-reactive protein (CRP), immunoglobulin G(IgG), immunoglobulin G(IgA), immunoglobulin M(IgM), IL-1β, IL-6 and IL-18 in combine group were lower (P<0.05). Conclusion: Jiawei shaogan fuzi decoction combined with mild moxibustion can improve clinical treatment effect, regulate immune func- tion and inhibit serum IL-1β, IL-6 and IL-18 levels in patients with active RA of cold-dampness obstruction type. [ABSTRACT FROM AUTHOR]

Objective: To observe the effect of carrelizumab combined with albumin-bound paclitaxel on immune function, tumor markers and angiogenesis indexes in patients with advanced cervical cancer. Methods: According to the random number table method, 93 patients with advanced cervical cancer admitted to the Affiliated Huai'an Hospital of Xuzhou Medical University, Jiangsu Cancer Hospital and Zhongda Hospital Affiliated to Southeast University from June 2019 to June 2023 were divided into control group (n=46, albumin-bound paclitaxel treatment) and observation group (n=47, carrelizumab combine with albumin-bound paclitaxel treatment). The efficacy, tumor markers, immune function indexes, angiogenesis indexes and adverse reactions in two groups were compared. Results: The objective response rate (ORR) of the observation group was 44.68%, and the disease control rate (DCR) was 74.47%, which were higher than the control group's 21.74% and 50.00%, respectively(P<0.05). After treatment, the levels of squamous cell carcinoma antigen (SCCA), carcinoembryonic antigen (CEA), carbohydrate antigen (CA) 125, CA199, angiopoietin-2 (Ang-2), vascular endothelial growth factor (VEGF) and CD8+ in observation group were lower than those in control group, and the levels of CD3+, CD4+, CD4+/CD8+ were higher than those in control group (P<0.05). The total incidence of adverse reactions in control group was 34.78%, and that in observation group was 29.79%, there was no difference between two groups(P>0.05). Conclusion: Carrelizumab combined with albumin-bound paclitaxel can reduce the level of tumor markers in patients with advanced cervical cancer, improve immune function, inhibit tumor angiogenesis, and effectively prevent disease progression. [ABSTRACT FROM AUTHOR]

Objective: To compare the effects of dexmedetomidine and propofol on hemodynamics, stress response and immune function in children undergoing liver mass resection. Methods: The random number table method was used, 90 children who underwent elective liver mass resection in our hospital from May 2021 to April 2023 were divided into dexmedetomidine group (dexmedetomidine anesthesia) and propofol group (propofol anesthesia), 45 cases in each group. The changes of hemodynamics [heart rate (HR), mean arterial pressure (MAP)], stress response [cortisol (Cor), adrenocorticotropic hormone (ACTH)] and T lymphocyte subsets [CD4+, CD8+, CD4+/CD8+] were compared between two groups, and the incidence of adverse reactions during anesthesia was observed. Results: MAP in dexmedetomidine group was lower than that in propofol group at 10 min after anesthesia induction (T1) to the end of operation (T4), and HR was higher than that in propofol group(P<0.05). Cor and ACTH in dexmedetomidine group were lower than those in propofol group at T4 and 1 day after operation (T5) (P<0.05). CD8+ in dexmedetomidine group was lower than that in propofol group at T4 and T5, while CD4+ and CD4+/CD8+ were higher than those in propofol group (P<0.05). There was no difference in the incidence of adverse reactions between two groups(P>0.05). Conclusion: Compare with propofol anesthesia, dexmedetomidine can better stabilize hemodynamics, reduce stress response, and reduce immunosuppression in children undergoing liver mass resection. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of different doses of dexmedetomidine combined with ropivacaine transversus abdominis plane block (TAPB) on immune inflammatory response and cognitive function in patients undergoing laparoscopic inguinal hernia repair. Methods: 150 patients with laparoscopic inguinal hernia repair who were admitted to the Second People's Hospital of Hefei from March 2020 to March 2022 were divided into group A (n=75) and group B (n=75) according to the random number table method.Both groups were treated with dexmedetomidine combined with ropivacaine TAPB, group A was given 0.5 μg/kg dexmedetomidine and group B was given 1 μg/kg dexmedetomidine. The hemodynamics, visual analogue scale (VAS), mini-mental state examination (MMSE) score, incidence of postoperative cognitive dysfunction (POCD), inflammatory response and immune function related indicators were compared in two groups. Results: Compared with group A at the beginning of operation 1 h (T1) and immediately after the end of operaion (T2), mean arterial pressure (MAP) and heart rate (HR) were lower in group B (P<0.05). The incidence of POCD in group B was lower than that in group A (P<0.05). Compared with group A 1 d and 2 d after operation, the MMSE score in group B was higher (P<0.05). Compared with group A at 12 h, 24 h and 48 h after operation, the VAS score in group B was lower (P<0.05). Compared with group A 2 d after operation, CD8+ was lower, and CD3+, CD4+ and CD4+/CD8+ were higher in group B(P<0.05). Compared with group A 2 d after operation, tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and C-reactive protein (CRP) were lower in group B (P<0.05). Conclusion: Anesthesia with 1 μg/kg dose of dexmedetomidine combined with ropivacaine TAPB in patients undergoing laparoscopic inguinal hernia repair, which can reduce pain, alleviate the degree of immune inflammatory response, maintain hemodynamic stability, and reduce the incidence of POCD. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of Xingpi Yanger Granules combine with Saccharomyces boulardii sachets on the intestinal microecology and inflammatory immune response in children with rotavirus enteritis. Methods: 118 children with rotavirus enteritis admitted to Jinan Second Maternal and Child Health Hospital from June 2021 to June 2023 were selected, the children were randomly divided into control group (n=59) and observation group (n=59) by random number table method. The control group received treatment with Saccharomyces boulardii sachets, while the observation group received additional treatment with Xingpi Yanger granules on the basis of the control group. Evaluated the clinical efficacy, symptom improvement time, intestinal microecology indicators, inflammatory factors, immune function, and adverse reactions of two groups. Results: The total effective rate of the observation group was 91.53%, which was higher than the control group's 76.27%(P<0.05). The observation group had shorter diarrhea relief time, fever relief time, hospitalization time and bowel movement recovery time compared to the control group(P<0.05). After treatment, the observation group had higher levels of Bifidobacterium, Lactobacillus, and Escherichia coli than the control group (P<0.05). After treatment, the levels of C-reactive protein (CRP), Interleukin-1β (IL-1β), tumor necrosis factorα(TNFα), Interleukin-6(IL-6) in the observation group were lower than the control group (P<0.05). After treatment, the levels of CD3+, CD4+/CD8+, CD4+ in the observation group were higher than those in the control group, while CD8+ levels were lower than those in the control group (P<0.05). The incidence rates of adverse reactions in both groups were no significant difference(P>0.05). Conclusion: The combination of Xingpi Yanger Granules and Saccharomyces boulardii sachets has a good therapeutic effect on children with rotavirus enteritis, it can improve clinical symptoms, reduce the level of inflammatory factors, regulate the level of intestinal microecology, enhance the immune function of children, and has good safety. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of 0.5 μg/kg, 1.0 μg/kg, 1.5 μg/kg three doses of dexmedetomidine mix with ropivacaine ultrasound-guided thoracic paravertebral nerve block(TPVB) combine with general anesthesia on stress response, immune function and micrometastasis in patients undergoing thoracoscopic radical resection of lung cancer. Methods: 132 patients undergoing thoracoscopic radical resection of lung cancer in our hospital were selected, and patients were divided into high dose group (1.5 μg/kg dexmedetomidine combine with ropivacaine ultrasound-guided TPVB, 44 cases), middle dose group (1.0 μg/kg dexmedetomidine combine with ropivacaine ultrasound-guided TPVB, 44 cases) and low dose group (0.5 μg/kg dexmedetomidine combine with ropivacaine ultrasound-guided TPVB, 44 cases) according to the random number table. The recovery quality, hemodynamics [heart rate (HR), systolic blood pressure (SBP), diastolic blood pressure (DBP)], stress response indexes [epinephrine (E), norepinephrine (NE), cortisol (Cor)], immune function [CD3+, CD4+, CD8+, CD4+/CD8+], micrometastasis [epidermal growth factor receptor messenger ribonucleic acid (EGFR mRNA), lung specific X protein (LUNX mRNA), cytokeratin 19 (CK19 mRNA)] and incidence of adverse reactions were compared in three groups. Results: The recovery time, tracheal extubation time and recovery room stay time in low dose group, middle dose group and high dose group were prolonged in turn(P<0.05). HR, SBP and DBP in low dose group, middle dose group and high dose group increased in turn at the time of tracheal extubation (T2) and 5 min after tracheal extubation (T3) (P<0.05). The levels of E, NE and Cor in low dose group, middle dose group and high dose group increased in turn at 48h after operation (P<0.05). CD8+ in low dose group, middle dose group and high dose group increased in turn, while CD3+, CD4+ and CD4+/CD8+ decreased in turn at 48 h after operation (P<0.05). EGFR mRNA, LUNX mRNA, and CK19 mRNA decreased at 48 h after surgery in the three groups, but there was no significant difference between the three groups.There was no difference in the incidence of adverse reactions in three groups(P>0.05). Conclusion: Dexmedetomidine combine with ropivacaine ultrasound-guided TPVB can effectively control the stress response of patients undergoing thoracoscopic radical resection of lung cancer, maintain hemodynamic fluctuations, reduce immunosuppression, and improve micrometastasis, the dose of 0.5 μg/kg is the best. [ABSTRACT FROM AUTHOR]

Objective: To observe the effects of frailty on anxiety and depression, immune function and cognitive function in elderly patients with rheumatoid arthritis (RA). Methods: 138 RA patients who were admitted to our hospital from January 2019 to December 2021 were selected, and patients were divided into frailty group and non-frailty group according to whether frailty occurred. The changes of anxiety and depression scores, immune function related indicators and cognitive function scores in frailty group and non-frailty group were compared. Results: Among 138 elderly RA patients, 91 had frailty, and 47 had no frailty. Patients were divided into frailty group (n=91) and non-frailty group (n=47) according to whether they were frail or not. The scores of self-rating anxiety scale (SAS) and self-rating depression scale (SDS) in frailty group were higher than those in non-frailty group(P<0.05). CD3+, CD4+ and CD4+/CD8+ in frailty group were lower than those in non-frailty group, and CD8+ was higher than that in non-frailty group (P<0.05). The Mini-Mental State Examination (MMSE) score and Montreal Cognitive Assessment (MoCA) score in frailty group were lower than those in non-frailty group (P<0.05). Conclusion: Frailty can increase the degree of anxiety and depression in elderly RA patients, and can also lead to a decline in immune function and cognitive function. [ABSTRACT FROM AUTHOR]

Objective: To observe the effects of qidong yixin oral liquid combined with trimetazidine hydrochloride tablets on myocardial injury indexes, immune function and oxidative stress in elderly patients with coronary heart disease and angina pectoris. Methods: 118 patients with angina pectoris of coronary heart disease admitted to our hospital from December 2020 to December 2022 were divided into control group (59 cases, treated with trimetazidine hydrochloride tablets) and study group (59 cases, treated with qidong yixin oral liquid combined with trimetazidine hydrochloride tablets) according to the random number table method. The cardiac function indexes, myocardial injury indexes, immune function indexes and oxidative stress indexes in two groups were compared. Results: The malondialdehyde (MDA), creatine kinase isoenzyme (CK-MB), cardiac troponin (cTnI), brain natriuretic peptide (BNP), CD8+ were lower in study group 4 weeks after treatment, the left ventricular end-diastolic diameter (LVEDd) and left ventricular end-systolic diameter (LVESd) were smaller, and the superoxide dismutase (SOD), left ventricular ejection fraction (LVEF), CD3+, CD4+, CD4+/CD8+ were higher (P<0.05). Conclusion: Qidong Yixin oral liquid combined with trimetazidine hydrochloride tablets can effectively improve cardiac function, myocardial injury, immune function and oxidative stress indexes in elderly patients with coronary heart disease and angina pectoris [ABSTRACT FROM AUTHOR]

Objective: To observe the clinical efficacy of xindili monoclonal antibody injection combined with S-1 and oxaliplatin (SOX) regimen as neoadjuvant therapy for locally advanced gastric cancer. Methods: According to the random number table method, 98 patients with locally advanced gastric cancer received in Xuzhou Cancer Hospital from January 2022 to June 2023 were divided into control group (49 cases treated with SOX regimen) and study group (49 cases treated with xindili monoclonal antibody injection on the basis of control group). The efficacy [objective response rate (ORR), disease control rate (DCR)], serum tumor marker levels, T lymphocyte subsets and incidence of adverse reactions were compared between two groups. Results: In study group, the ORR and DCR were higher than those in control group (P<0.05). After treatment, CEA, CA724, CA125 and CD8+ in study group were lower than those in control group, CD4+ and CD4+/CD8+ were higher than those in control group(P<0.05). The incidence of adverse reactions in the study group was 32.65% higher than that in the control group, which was 28.57%, but there was no difference between the two groups (P>0.05). Conclusion: Xindili monoclonal antibody injection combined with SOX regimen neoadjuvant therapy for locally advanced gastric cancer, which can effectively prevent disease progression, reduce serum tumor marker levels, improve immune function. [ABSTRACT FROM AUTHOR]

Obejective: To investigate the effects of different doses of protein diet on nutritional status, immune function and oxidative stress in patients with type 2 diabetes mellitus (T2DM) with pulmonary tuberculosis. Methods: 120 T2DM patients with pulmonary tuberculosis who were admitted to Hunan Provincial Chest Hospital from March 2020 to December 2021 were selected as research objects. Patients were randomly divided into high protein group (1.5 g/kg/d protein, total calorie increased by 10%), medium protein group (1.2 g/kg/d protein, total calorie increased by 10%), low protein group (1.0 g/kg/d protein, total calorie increased by 10%). 40 cases in each group. The sputum mycobacterium tuberculosis negative conversion, pulmonary infection, blood glucose, nutritional status, immune function and oxidative stress level in three groups were compared. Results: The sputum mycobacterium tuberculosis negative conversion rate in high protein group and medium protein group was higher than that in low protein group, and high protein group was higher than that of medium protein group (P<0.05). The pulmonary infection rate in high protein group and medium protein group was lower than that in low protein group, and high protein group was lower than that of medium protein group (P<0.05). After treatment, the glycosylated hemoglobin (HbA1c), fasting blood glucose (FBG) and 2h postprandial blood glucose (2hPG) in high protein group and medium protein group were lower than those in low protein group, and high protein group was lower than that of medium protein group (P<0.05). After treatment, total albumin (TP), prealbumin (PA) and albumin (ALB) in high protein group and medium protein group were higher than those in low protein group, and high protein group was higher than that of medium protein group (P<0. 05). After treatment, CD3+, CD4+ and CD4+ /CD8+ in high protein group and medium protein group were higher than those in low protein group, and high protein group was higher than that of medium protein group (P<0.05). After treatment, CD8+ in high protein group and medium protein group was lower than that in low protein group, and high protein group was lower than that of medium protein group (P<0.05). After treatment, the superoxide dismutase (SOD) in high protein group and medium protein group was higher than that in low protein group, and high protein group was higher than that of medium protein group (P<0.05). After treatment, the malondialdehyde (MDA) in high protein group and medium protein group was lower than that in low protein group, and high protein group was lower than that of medium protein group (P<0.05). Conclusion: High protein diet can improve the nutritional status and immune function of T2DM patients with pulmonary tuberculosis, and reduce the level of oxidative stress. [ABSTRACT FROM AUTHOR]

Objective: Effects of Trichoderma on intestinal microecology, gastrointestinal function and immune function of gastric cancer rats through p53 signaling pathway. Methods: 40 male SD rats were randomly divided into model group, low dose group, middle dose group and high dose group, 10 rats in each group. Another 10 healthy male rats were selected and divided into blank group. After successful modeling, the blank group and the model group were given the same amount of normal saline by gavage, and the low, medium and high dose groups of trichoderma alcohol were given 20, 40 and 60 mg / kg by gavage. The expression of p53 protein was detected by Western blot. The changes of intestinal flora were detected by 16 S rDNA assay. The levels of serum gastrointestinal function indexes were detected by enzyme-linked immunosorbent assay (ELISA). Peripheral blood T lymphocyte subsets were detected by flow cytometry. Results: The expression level of p53 protein in the model group and the low, medium and high dose groups was lower than that in the blank group, and the p53 protein level in the medium dose group was higher than that in the model group and the low and high dose groups (P<0.05). The levels of Firmicutes, Bacteroidetes and Proteobacteria in the model group and Trichoderma alcohol (low, medium and high dose) groups were significantly higher than those in the blank group. The levels of Firmicutes, Bacteroidetes and Proteobacteria in the medium dose group were significantly lower than those in the model group and Trichoderma alcohol (low and high dose) groups (P<0.05). The levels of gastrin and motilin in the model group and the trichoderma alcohol (low, medium and high dose) group were significantly lower than those in the blank group, and the levels of gastrin (GAS) and motilin (MTL) in the middle and low dose group of trichoderma alcohol were significantly higher than those in the model group and the trichoderma alcohol (low and high dose) group (P<0. 05). Compared with the blank group, the levels of CD3+ and CD4+ in the model group and the Trichoderma alcohol (low, medium and high dose) groups were higher, and the level of CD8+ was lower. Compared with the model group and the low-dose and high-dose groups, the levels of CD3+ and CD4+ in the low-dose group were lower, and the level of CD8+ was higher (P<0.05). Conclusion: Trichoderma alcohol can effectively regulate the intestinal flora of gastric cancer rats, enhance their immune function, and promote the recovery of intestinal function, and the medium dose application effect is better. [ABSTRACT FROM AUTHOR]

To investigate the effect of zhengyuan capsule combine with anlotinib on efficacy, cancer-related fatigue and immune function in patients with advanced non-small cell lung cancer (NSCLC) after second-line treatment. 103 patients with advanced NSCLC who were progressed driver gene negative after second-line treatment in Jinling Hospital Affiliated to Medical School of Nanjing University from April 2020 to May 2023 were selected, and patients were divided into control group (treated with anlotinib, n=51) and study group (treated with zhengyuan capsules combine with anlotinib, n=52) by random number table method. The clinical efficacy, Piper fatigue scale, Karnofsky functional status score (KPS), serum tumor markers [cytokeratin 19 fragment antigen 21-1 (CYFRA21-1), carcinoembryonic antigen (CEA), carbohydrate antigen 125 (CA125)], immune function indicators [natural killer (NK) cells, T lymphocyte subsets: CD4+, CD8+, CD4+/CD8+] were compared between two groups, and the occurrence of adverse reactions during the treatment in two groups was observed. Compared with control group, the objective response rate (ORR) and disease control rate (DCR) in study group were higher (P<0.05). The Piper fatigue scale score in study group after treatment was lower than that in control group, KPS score was higher than that in control group. The decrease levels of serum tumor markers CYFRA21-1, CEA, and CA125 in study group were higher than those in control group (P<0.05), immune function indicators, the decrease rate of NK cells, CD4+, CD4+/CD8+ in study group was lower than those in control group, and the increase rate of CD8+ was lower than that in control group (P<0.05). There was no difference in the incidence of adverse reactions between two groups during treatment (P>0.05). Zhenyuan capsule combine with anlotinib can improve the clinical efficacy of advanced NSCLC patients with progression driver gene negative after second-line treatment, which can reducing the level of tumor markers, reducing cancer-related fatigue, improving quality of life and improving immune function. [ABSTRACT FROM AUTHOR]

Objective: To observe the effects of electroacupuncture stimulation anesthesia at acupoints on anesthesia effect, stress response and immune function in elderly patients undergoing radical resection of rectal cancer. Methods: 97 elderly patients who underwent radical resection of rectal cancer in our hospital from January 2022 to May 2023 were divided into control group (n=48, total intravenous anesthesia) and observation group (n=49, on the basis of control group received electroacupuncture stimulation anesthesia at acupoints) by random number table method.The additional amount of anesthetic drugs, hemodynamics, pain score, Immune function indicators, stress response indicators and adverse reactions were compared between two groups. Results: Compared with control group, the dosage of vecuronium bromide, propofol and remifentanil in observation group were less(P<0.05). Compared with control group, the mean arterial pressure and respiratory rate in observation group were lower at the time points from anesthesia induction period (T1) to the end of anesthesia (T3) (P<0.05). Compared with control group, the pain visual analogue score (VAS) in observation group was lower at 6 h, 12 h and 24 h after operation(P<0.05). Compared with control group, CD8+ in observation group was lower, CD4+, natural killer (NK) cells, CD4+/CD8+ were higher, and norepinephrine, adrenal gland and cortisol were lower (P<0.05). There was no difference in the incidence of adverse reactions between two groups(P>0.05). Conclusion: Electroacupuncture stimulation anesthesia at acupoints for elderly patients undergoing radical resection of rectal cancer, which can reduce the dosage of other anesthetics, relieve pain, maintain hemodynamic stability,reduce stress response, improve immunosuppression, and have good anesthetic effect. [ABSTRACT FROM AUTHOR]

Objective:To investigate the effects of acute hypervolemic hemodilution (AHH) combined with control low central venous pressure (CLCVP) on inflammation,immune regulation and blood protection in patients undergoing posterior spinal operation.Methods:120 patients who were underwent posterior spinal operation in Nanjing Drum Tower Hospital from January 2020 to December2022 were selected as the research objects,patients were divided into control group,AHH group and AHH+CLCVP group according to the random number table method,with 40 cases in each group.Control group was given lactated Ringer’s solution hemodilution,AHH group was given AHH blood management,and AHH+CLCVP group was given AHH combine with CLCVP blood management.The changes of perioperative indexes,hemodynamic indexes,inflammatory factors indexes and immune function indexes were compared in three groups.Results:The intraoperative blood loss and suspended red blood cell transfusion volume in AHH+CLCVP group were lower than those in AHH group and control group (P＜0.05),the intraoperative blood loss and suspended red blood cell transfusion volume in AHH group were lower than those in control group (P＜0.05).Compared with T1time point,the heart rate (HR) in three groups increased first and then decreased at 60 min after the start of operation (T2)～the end of operation (T3)(P＜0.05),and the mean arterial pressure(MAP) decreased first and then increased (P＜0.05),the central venous pressure (CVP) in control group and AHH group increased first and then decreased at T2～T3(P＜0.05),and the CVP in AHH+CLCVP group remained stable at T0～T3(P＞0.05).HR in AHH+CLCVP group was lower than that in AHH group and control group at T2～T3(P＜0.05),MAP was higher than that in AHH group and control group (P＜0.05),CVP was lower than that in AHH group and control group (P＜0.05),HR in AHH group was lower than that in control group at T2～T3(P＜0.05),MAP was higher than that in control group (P＜0.05).Compared with T1time,the levels of serum procalcitonin (PCT),C-reactive protein (CRP) and interleukin-6 (IL-6) in three groups,peripheral blood CD8+increased first and then decreased at T2～24h after operation (T4)(P＜0.05),peripheral blood CD3+,CD4+,CD4+/CD8+decreased first and then increased at T2～T4(P＜0.05).The levels of serum PCT,CRP,IL-6 and peripheral blood CD8+in AHH+CLCVP group were lower than those in AHH group and control group at T2～T4(P＜0.05),and the levels of peripheral blood CD3+,CD4+and CD4+/CD8+were higher than those in AHH group and control group (P＜0.05),the levels of serum PCT,CRP,IL-6 and peripheral blood CD8+in AHH group were lower than those in control group at T2～T4(P＜0.05),and the levels of peripheral blood CD3+,CD4+and CD4+/CD8+were higher than those incontrol group(P＜0.05).Conclusion: AHH combined with CLCVP can stabilize hemodynamics, reduce intraoperative blood loss and blood transfusion, reduce inflammatory response and improve immune function in patients undergoing posterior spinal operation. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of enteral nutrition (EN) based on energy metabolism monitoring on intestinal function, nutritional status and immune function in elderly critically ill patients. Methods: According to the random number table method,136 elderly critically ill patients who were admitted to of EICU Department of Nanjing First Hospital from April 2020 to January 2023 were divided into control group (routine EN) and study group (routine EN based on energy metabolism monitoring), with 68 cases in each group. The clinical indexes, nutritional status, immune function indexes, intestinal function indexes and incidence of complications were compared between two groups. Results: The EN compliance rate within 7 days and the success rate of weaning within 7 days in study group were higher than those in control group, the ICU hospitalization time and mechanical ventilation time in study group were shorter than those in control group, and the total incidence of complications was lower than that in control group (P<0.05). 14 days after intervention, the levels of albumin (ALB), serum total protein (TP), prealbumin (PA) and hemoglobin (Hb) in study group were higher than those in control group (P<0.05). 14 days after intervention, the levels of immunoglobulin (Ig) M, IgG and IgA in study group were higher than those in control group (P<0.05). 14 days after intervention, the levels of D-lactic acid and diamine oxidase (DAO) in study group were lower than those in control group (P<0.05). Conclusion: EN intervention based on energy metabolism monitoring in elderly critically ill patients, which can further improve the nutritional status, intestinal function and immune function of patients. [ABSTRACT FROM AUTHOR]

Objective: To investigate the efficacy of carelizumab combined with capecitabine for maintenance therapy in first-line treatment of patients with recurrent metastatic nasopharyngeal carcinoma (NPC), and its impact on immune function of patients. Methods 80 patients with recurrent metastasis of NPC who were admitted to Qinzhou First People’s Hospital from January 2020 to January 2022 were selected, the recurrent and metastatic NPC patients were divided into the control group and the observation group according to the random number table method, each group has 40 cases. Both groups received gemcitabine combined with cisplatin chemotherapy, the control group received carelizumab monotherapy maintenance treatment for 1 year after chemotherapy, and the observation group received carelizumab combined with capecitabine maintenance treatment for 1 year after chemotherapy. Differences in efficacy, adverse reactions, and percentage of B lymphocyte subsets and natural killer cells (NK) before and after treatment were compared between the two groups. Results; The objective remission rate (ORR) and disease control rate (DCR) in observation group were higher than those in control group (P＜0. 05). The percentages of CD5+B cells, CD5+CD19+B cells and CD3-CD56+NK cells in peripheral blood after treatment was decreased between the two groups (P＜0. 05), but the percentages of CD5+B cells, CD5+CD19+B cells and CD3-CD56+NK cells in peripheral blood of the observation group were higher than those of the control group after treatment (P＜0. 05). There was no significant difference in the incidence of adverse reactions above grade Ⅲ between the two groups (P＞0. 05). Conclusion: Carelizumab combined with capecitabine in first-line maintenance treatment of recurrent and metastatic NPC could improve clinical efficacy, reduce the impact on cellular immune function, and it was safe and reliable. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of Camrelizumab combine with chemotherapy on immune function, neutrophil to lymphocyte ratio (NLR), C-reactive protein to albumin ratio (CAR) in patients with advanced non-squamous non-small cell lung cancer (NSCLC). Methods: Used retrospective analysis, 100 patients with advanced non-squamous NSCLC who were admitted to the first affiliated hospital of Anhui medical university north district from January 2020 to December 2022 were selected, and patients were divided into control group (n=48) and observation group (n=52) according to the treatment methods. Control group received chemotherapy with pemetrexed disodium combined with cisplatin injection or nedaplatin for injection, and observation group received Camrelizumab treatment on the basis of control group. The efficacy, immune function, tumor markers, NLR, and CAR were compared between two groups, and the incidence of adverse reactions during treatment were observed simultaneously in two groups. Results: Compared with control group, the clinical total effective rate in observation group was higher (P<0.05). 4 cycles after treatment, compared with control group, the carcinoembryonic antigen (CEA), cytokeratin 19 fragment antigen 21-1 (CYFRA21-1), carbohydrate antigen 125 (CA125), NLR, CAR and CD8+ in observation group were lower, while CD3+, CD4+ and CD4+/CD8+ were higher (P<0.05). There was no difference in the incidence of adverse reactions between two groups (P>0.05). Conclusion: Patients with advanced non-squamous NSCLC combine with Camrelizumab treatment on the basis of chemotherapy, which is helpful to control disease progression, improve clinical efficacy, reduce tumor markers levels, improve immune function, and reduce NLR and CAR. [ABSTRACT FROM AUTHOR]

Objective:To explore the efficacy of carboplatin and pemetrexed combined with pablizumab in the treatment of advanced non squamous non-small cell lung cancer （NSCLC） and its effects on cellular immune function and tumor markers. Methods78 patients with advanced non squamous NSCLC who were admitted to the Affiliated Hospital of Xuzhou Medical University from October2018 to February 2021 were selected, they were divide into control group （n=39, received pemetrexed combined with carboplatin treatment） and combined group（n=39, received pembrolizumab treatment on the basis of control group） according to the random number table method. The efficacy, tumor marker levels, cellular immune function indicators, incidence of adverse reactions. Results: The objective response rate and disease control rate of combined group were higher than those of control group（P＜0. 05). There was no statistically significant difference in the incidence of adverse reactions between two groups（P＞0. 05). After treatment, the carcinoembryonic antigen （CEA）cytokeratin 19 fragment （CYFRA21-1）, and carbohydrate antigen 125 （CA125） levels decreased of two groups, and combined group were lower than those of control group （P＜0. 05). After treatment, the CD3+, CD4+, and CD4+/CD8+ decreased of two groups, but combined group were higher than those of control group （P＜0. 05). After treatment, the CD8+ increased of two groups, but combined group was lower than that of control group （P＜0. 05). Conclusion: Pabolizumab combined with pemetrexed and carboplatin in the treatment of advanced non-squamous NSCLC can effectively control disease progression, improve patient immune function, and reduce CEA, CYFRA21-1, and CA125 levels. [ABSTRACT FROM AUTHOR]

To explore the efficacy of vitamin D drops combined with budesonide aerosol in the treatment of children with asthma and its effects on lung function, immune function and inflammatory factors. 120 children with asthma admitted to our hospital from August 2019 to December 2022 were randomly divided into observation group and control group, 60 cases in each group. The control group was treated with budesonide aerosol; on the basis of the control group, the observation group was treated with vitamin D drops. The clinical efficacy and clinical symptoms, lung function [forced expiratory volume in the first second (FEV1), peak expiratory flow (PEF), PEF diurnal variation rate], immune function [regulatory T cells (Treg), helper T cells 2 (Th2), Th17] and inflammatory factors [tumor necrosis factor-琢 (TNF-琢), interleukin-6 (IL-6), IL-8] levels before and after treatment were compared between the two groups. The total effective rate of the observation group was higher comparing with the control group (91.67% vs 73.33%, P<0.05). Compared with the control group, the scores of daytime symptoms and nighttime symptoms in the observation group were lower, and the C-ACT score was higher (P<0.05). Compared with the control group, the percentage of FEV1, PEF and Treg in the observation group was higher, and the diurnal variation rate of PEF, the percentage of Th2 and Th17 were lower (P<0.05). After treatment, the percentages of Treg, Th2 and Th17 in the observation group were lower comparing with the control group (P<0.05). The levels of serum TNF-琢, IL-6 and IL-8 in the observation group were significantly lower comparing with the control group (P<0.05). Vitamin D drops combined with budesonide aerosol is effective in the treatment of children with asthma, which can significantly improve the clinical symptoms and lung function, immune function and reduce the level of inflammatory factors. [ABSTRACT FROM AUTHOR]

Objective: To analyze the value of preoperative DSA injury grading in splenic artery embolization for traumatic splenic rupture. Method: A total of 95 patients with traumatic splenic rupture admitted to our hospital from January 2020 to December 2022 were selected for study. According to the random number table method, they were divided into routine operation group (45 cases) and DSA-guided splenic artery embolization group (50 cases). The routine operation group underwent open total splenectomy, and the DSA-guided splenic artery embolization group underwent DSA-guided splenic artery embolization. The operation-related indexes, T lymphocytes (CD3', CD4", CD8', CD4/CD8'), immunoglobulin (IgG, IgA, IgM), white blood cell (WBC) and platelet (PLT) contents, serum interleukin 6 (IL-6), serum interleukin 10 (IL-10) and tuftsin levels of different DSA grades in the two groups and DSA-guided splenic artery embolization group were analyzed. Results: Compared with the conventional operation group, the splenic artery embolization group under DSA guidance had shorter operation time, bed time and hospital stay, and less intraoperative blood loss (P<0. 05). On postoperative day 14, compared with the conventional operation group, the levels of CD3, CD4", CD4/CD8 in the DSA-guided splenic artery embolization group were higher, and the levels of CD8 were lower (P<0.05). On day 14 after surgery, the levels of IgG, IgA and IgM in the DSA-guided splenic artery embolization group were higher than those in the conventional operation group (P<0.05). At 14 days after operation, PLT content in the DSA-guided splenic artery embolization group was lower than that in the conventional operation group. Compared with DSAIII, the PLT content of DSA III was lower (P<0.05). On the 14 th day after operation, compared with the conventional operation group, the serum IL-6 level in the DSA-guided splenic artery embolization group was lower, and the serum IL-10 and Tuftsin levels were higher (P<0.05). Conclusion: Under the guidance of DSA injury grading, splenic artery embolization for patients with traumatic splenic rupture has little effect on the immune function of the body, and there will be no massive bleeding during the operation, which can not only reduce the platelet content of the patients, but also promote the recovery of the patients as soon as possible. INSET: +.. [ABSTRACT FROM AUTHOR]

Objective: To investigate the analgesic effect of thoracic paravertebral nerve block (TPVB) combined with general anesthesia in liver cancer resection. Methods: 120 patients undergoing liver cancer resection were divided into a control group and a TPVB group based on different anesthesia methods, with 60 patients in each group. The control group received simple general anesthesia, and the TPVB group received TPVB combined with general anesthesia. The pain numerical rating scale (NRS) scores of the two groups were recorded at 4 h, 12 h, 24 h and 48 h after operation. The use of anesthetic drugs and postoperative analgesia related indicators in the two groups were recorded. The adverse reactions, first exhaust time and hospitalization time of the two groups were recorded. The serum levels of interleukin-6 (IL-6) and IL-8 were detected before and 24 hours after operation, and the peripheral blood T lymphocyte subsets were measured by flow cytometry. Results: Compared with the control group, the NRS scores of the TPVB group at 4 h, 12 h and 24 h after operation were significantly decreased (P<0.05). Compared with the control group, the amount of remifentanil used in the TPVB group was reduced (P<0.05), the time of first pressing the analgesic pump was delayed (P<0.05), the number and amount of analgesic pressing at 24 h after operation were reduced (P<0.05), and the remedial analgesia rate was significantly reduced (P<0.05). The first exhaust time in the TPVB group was earlier than that in the control group (P<0.05), the hospitalization time was shorter than that in the control group (P<0.05), and the incidence of postoperative nausea and vomiting was significantly lower than that in the control group (P<0.05). At 24 h after operation, the levels of IL-6 and IL-8 in the two groups were higher than those before operation (P<0.05), but the TPVB group was lower than the control group (P<0.05). At 24 h after operation, the levels of CD3+, CD4+ and CD4+ /CD8+ in the two groups were lower than those before operation (P<0.05), but those in the TPVB group were higher than those in the control group (P<0. 05). Conclusion: TPVB combined with general anesthesia for liver cancer resection can improve postoperative analgesia, reduce perioperative opioid consumption, reduce postoperative inflammatory response, improve postoperative immune function, and have a positive effect on postoperative recovery. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of imperatorin (IMP) on the immune function of bladder cancer bearing mice by regulating the growth arrest specific protein 6 (Gas6)/Axl axis. Method: 12 mice were randomly selected from 60 SPF grade male C57BL/6 mice as the control group (CON group). The remaining mice were evenly divided into Model group, IMP group, Gas6 group, and IMP+Gas6 group, with 12 mice in each group. A concentration of 0.2 mL was injected subcutaneously into their left forelimb at a concentration of 1x107/mL murine bladder cancer cell line T739 cell solution (model group and IMP group were injected with T739 stable transfection cell line of control vector, Gas6 group and IMP+Gas6 group were injected with T739 stable transfection cell line expressing Gas6) for modeling. After successful modeling, the IMP group and IMP+Gas6 group received intraperitoneal injection of 5 mg/kg of IMP. The CON group, Model group, and Gas6 group were injected with an equal amount of physiological saline via intraperitoneal injection, once every 2 days, for 5 consecutive times. Measure the volume and weight of mouse tumors. Measure thymus index and spleen index. Serum interleukin-2 (IL-2), y Interferon (IFN-y), vascular endothelial growth factor (VEGF) levels were detected by enzyme linked immunosorbent assay (ELISA). Hematoxylin eosin (HE) staining was used to examine the pathological changes of tumor tissue. T cell subpopulations was detected by flow cytometry. TUNEL staining was used to detect cell apoptosis. Western blot (Western blot) was used to detect the expression of Gas6/Axl pathway related proteins. Results: The tumor cells in Model group were loosely arranged, with pyknosis and Karyorrhexis in different degrees. IMP can reverse this phenomenon, making the tumor cell arrangement become compact again, the degree of nuclear pyknosis increases, and the Karyorrhexis phenomenon decreases. The model group showed a significant increase in tumor volume, tumor mass, CD8+ cell percentage, Gas6, Axl protein levels, and VEGF levels compared to the CON group (P<0.05), as well as thymus and spleen indices, IL-2, and IFN-酌, the percentage of CD4+ cells, CD4+ /CD8+, and tumor cell apoptosis rate significantly decreased (P<0.05). Compared with the Model group, the IMP group had thymic index, spleen index, IL-2, IFN-酌, the percentage of CD4+ cells, CD4+ /CD8+ cells, and tumor cell apoptosis rate significantly increased (P<0.05), while tumor volume, tumor mass, percentage of CD8+ cells, Gas6, Axl protein levels, and VEGF levels significantly decreased (P<0.05). The results of the Gas6 group showed opposite trends to those of the IMP group. The results of IMP+Gas6 group showed that IMP could reverse the tumor promoting effect of overexpression of Gas6 on bladder cancer bearing mice. IMP may improve the immune function of bladder cancer bearing mice by down-regulation of Gas6/Axl signaling pathway, thus producing anti-tumor effect. [ABSTRACT FROM AUTHOR]

Objective: To explore the efficacy of Qingre Liyan decoction combine with Xiyanping injection in the treatment of children with herpangina and its influence on inflammatory factors and immune function. Methods: 83 children with herpangina who were admitted to Shijiazhuang Traditional Chinese Medicine Hospital from May 2019 to February 2022 were selected, and they were divided into control group (n=41) and study group (n=42) according to the random number table method. On the basis of routine treatment, control group received treatment with Xiyanping injection, and study group received treatment with Qingre Liyan decoction on the basis of control group. The traditional Chinese medicine efficacy, clinical symptom improvement, inflammatory factors, and immune function changes were compared between two groups. Results: The clinical total effective rate in study group was higher than that in control group (P<0.05). The herpes healing time, fever relief time, and pharyngeal pain improvement time in study group were shorter than those in control group (P<0.05). 5 d after treatment, the interleukin-10 (IL-10) increased, γ- interferon (IFN-γ), interleukin-6 (IL-6) and C-reactive protein (CRP) decreased in two groups, and the improvement degree in study group was greater than that in control group (P<0.05). 5 d after treatment, the CD3+, CD4+, CD4+/CD8+, and NK increased in two groups, and the improvement degree in study group was greater than that in control group(P<0.05). Conclusion: Qingre Liyan decoction combine with Xiyanping injection in the treatment of children with herpangina, which can shorten the symptom relief time, reduce the level of inflammatory factors, improve the body's immune function, and improve clinical efficacy. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of intracavitary perfusion of compound Sophora flavescens injection on the immune function and the levels of tumor markers in patients with malignant pleural effusion. Methods: 52 patients with malignant pleural effusionwho were treated in The First Affiliated Hospital of Hebei North University from December 2020 to December 2022 were selected. Each patient was randomly numbered to obtain 1-52 numbers, and were divided into control group (n=26) and observation group (n=26) according to the parity method. The control group were treated with cisplatin by intracavitary perfusion, the observation group were treated with compound Sophora flavescens injection by intracavitary perfusion on the basis of the control group. The treatment effect, T lymphocyte subsets (CD3+, CD4+, CD4+/CD8+), carcinoembryonic antigen (CEA), carbohydrate antigen 125 (CA125), quality of life improvement rate and toxic and side effects were compared between the two groups. Results: The total effective rate of treatment in the observation group was 73.08% higher than 42.31% in the control group(P<0.05). After treatment, the CD3+, CD4+ and CD4+/CD8+ in the observation group were higher than those before treatment, and higher than those in the control group(P<0.05). After treatment, the levels of serum CEA and CA125 in the two groups were significantly lower than those before treatment, and the observation group were lower than that of the control group(P<0.05). The improvement rate of quality of life in the observation group was 76.92% higher than that 46.15% in the control group(P<0.05). The myelosuppression and gastrointestinal reaction in the observation group were significantly lighter than that of the control group (P<0.05). Conclusion: Intracavitary perfusion of compound Sophora flavescens injection has a good effect on patients with malignant pleural effusion, and it can improve immune function and quality of life, reduce serum the levels of tumor markers and toxic and side effects. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of Puji Zhichuang suppository combined with Huazhuo Jiedu formula retention enema in the treatment of anal sinusitis and its influence on pain mediators, immune function and recurrence rate. Methods: 100 patients with anal sinusitis who were diagnosed in our hospital from January 2020 to July 2021 were selected. According to the random number table method, they were divided into control group (treated with Puji Zhichuang suppository, 50 cases) and experimental group (Puji Zhichuang suppository combined with Huazhuo Jiedu formula retention enema treatment, 50 cases). Changes in efficacy, traditional Chinese medical syndrome score, pain mediators, immune function and recurrence rate in the two groups were compared. Results: The total effective rate in the experimental group was higher than that in the control group (P<0.05). After 2 courses of treatment, the scores of fallen anus and dull pain, anal incomplete defecation, and anal dampness in the experimental group were lower than those of the control group (P<0.05). CD8+ in the experimental group after 2 courses of treatment was lower than that in the control group. CD3+, CD4+, CD4+ /CD8+ in the experimental group were higher than those of the control group (P<0.05). After 2 courses of treatment, prostaglandin E2 (PGE2), Substance P (SP), dopamine (DA) and 5-hydroxytryptamine (5-HT) in the experimental group were lower than those of the control group (P<0.05). The recurrence rate in the experimental group at 4 months and 6 months after treatment were lower than those in the control group (P<0.05). Conclusion: Puji Zhichuang suppository combined with Huazhuo Jiedu formula retention enema in the treatment of anal sinusitis can promote the improvement of clinical symptoms, and reduce the recurrence rate, which may be related to reducing the pain mediators level and regulating the immune function of the body. [ABSTRACT FROM AUTHOR]

Objective: To analyze the risk factors of preoperative malnutrition in children with congenital heart disease (CHD), and to observe the effect of preoperative malnutrition on postoperative clinical outcome, immune function and quality of life of children. 65 children with CHD who were admitted to Jiangsu Provincial People's Hospital from January 2020 to June 2022 were selected, the nutritional status of children with CHD was assessed using the relevant nutritional diagnostic criteria developed by the National Center for Health Statistics of the United States, as recommended by the World Health Organization (WHO), and the incidence of preoperative malnutrition of children with CHD was calculated. Univariate and multivariate Logistic regression were used to analyze the risk factors of preoperative malnutrition in children with CHD, and the effects of preoperative malnutrition on postoperative clinical outcome, immune function and quality of life were also observed. Results: Among the enrolled children in this study, 31 had preoperative malnutrition, and 34 had no preoperative malnutrition. According to their nutritional status, the children were divided into malnutrition group (n=31) and non-malnutrition group (n=34). Multivariate Logistic regression analysis showed that low birth weight at birth, maternal anxiety and maternal depression were risk factors for preoperative malnutrition in children with CHD, and maternal initiative to acquire disease knowledge was the protective factor (P<0.05). There was no significant difference in the incidence of complications in the two groups (P>0.05). The length of hospital stay in the malnutrition group was longer than that in the non-malnutrition group, and the hospitalization cost was higher than that in the non-malnutrition group (P<0.05). 3 months after operation, CD3+, CD4+, CD4+ /CD8+ increased in the two groups, while CD8+ decreased, and the improvement effect in the non-malnutrition group was better than that in the malnutrition group (P<0.05). Preoperative communication problems, heart problems and symptoms, treatment anxiety, perceived physical appearance and cognitive psychological problems scores were all increased in the two groups at 3 months after operation, and the improvement effect in the non-malnutrition group was better than that in the malnutrition group (P<0.05). Conclusion: Low birth weight at birth, maternal anxiety and maternal depression are risk factors for preoperative malnutrition in children with CHD, while maternal initiative to acquire disease knowledge is a protective factor for preoperative malnutrition in children with CHD. Preoperative malnutrition will lead to postoperative decreased immune function, reduced quality of life, and relatively poor clinical outcome in children with CHD. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of different proportions of enteral combined with parenteral nutrition treatment on patients with severe traumatic brain injury (s TBI). Methods: The subjects of study were s TBI patients admitted to the Neurointensive Care Unit of Chongqing Emergency Medical Center from October 2020 to August 2022. 70 patients who met the inclusion and exclusion criteria were randomly divided into the experimental group and the control group with 35 cases each. Calculate the daily caloric requirements of patients and provide nutritional support according to different proportion of enteral and parenteral nutrition programs. The experimental group was given enteral and parenteral nutrition support at a ratio of 2:1, while the control group was given enteral and parenteral nutrition support at a ratio of 1:1. The differences of nutritional status indicators (hemoglobin, albumin, prealbumin, total protein), immune function indicators [CD3, CD4, CD4/CD8, immunoglobulin (Ig)A, Ig M, Ig G], inflammatory indicators [C-reactive protein (CRP), procalcitonin (PCT), interleukin-6 (IL-6)] and complications between the two groups were statistically compared. Results: In terms of immune function, the levels of CD4, CD4/CD8 and Ig A in the experimental group were significantly higher than those in the control group 14 d after nutritional support (P＜0. 05); There was no significant difference between the two groups in nutritional status, inflammatory indicators and complications (P＞0. 05). Conclusions: Enteral nutrition-based combined nutritional support can promote the recovery of immune function in patients with s TBI, but there is no significant difference in nutritional status, inflammatory indicators and complications between different proportions of enteral combined parenteral nutrition treatment. [ABSTRACT FROM AUTHOR]

Objective: To investigate the influences of astragalus polysacharide(APS) on anti-tumor effect and immune function of esophageal carcinoma(EPC) rats through phosphatidylinositol-3-kinase/protein kinase B (PI3K/Akt) signal pathway. Methods: 50 healthy 6-week-old SPF grade SD rats were randomly divided into model group(group M), cisplatin group(group S), APS low dosage group(group APSL), APS middle dosage group(group APSM) and APS high dosage group(group APSH), with 10 rats in each group. EPC model rats were established by transplantation of Eca109 cells, and 3mg/kg cisplatin and 100, 200, 400 mg/kg APS were given to each rat. Tumor volume and tumor mass were detected, tumor growth inhibition rate, thymus index and spleen index were calculated, histomorphology was observed by HE staining, CD3+, CD4+, CD8+T lymphocyte population were detected by flow cytometer, the relative expressions of PI3K and Akt protein were detected by Western blot. Results: After intervention, tumor volume and tumor mass of group S and group APS were all lower than group M(P＜0.05); the tumor volume and tumor mass of group S and group APSH were all lower than group APSM and group APSL(P＜0.05); the tumor growth inhibition rate of each APS group were 45.59%, 32.35% and 17.65%. The thymus index and spleen index of group S were lower than group M(P＜0.05); the thymus index and spleen index of each APS group were all higher than group S(P＜0.05); while group APSH and APSM were higher than group M(P＜0.05). The CD3+, CD4+ and CD4+/CD8+ of each APS group were higher than group S and group M, while the CD8+ was lower(P＜0.05); group APSH, APSM and APSL also had statistical differences(P＜0.05). The relative expressions of PI3K/Akt signal pathway protein of group S and each APS group were lower than group M(P＜0.05); group APSH, APSM and APSL also had statistical differences(P＜0.05). Conclusion: APS exerts inhibition effect on EPC via regulation of PI3K/Akt signal pathway and immune function, which can provide new thought and theoretical basis for clinical treatment of EPC. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of gefitinib combined with platinum plus cyclophosphamide (PC) chemotherapy on immune function, apoptotic factors and tumor markers in patients with epidermal growth factor receptor (EGFR) mutation-positive advanced lung adenocarcinoma. Methods: 92 patients with EGFR-positive advanced lung adenocarcinoma who were admitted to our hospital from March 2018 to March 2020 were selected, and they were divided into the control group (PC chemotherapy) and the experimental group (gefitinib combined with PC chemotherapy) according to random number table method, with 46 cases each. Efficacy, tumor markers, immune function, apoptotic factors change of situation, progression-free survival time (PFS), overall survival time (OS) and the incidence of adverse reactions were observed in the two groups. Results: The objective remission rate and disease control rate in the experimental group were higher than those in the control group (P<0.05). The levels of serum carcinoembryonic antigen (CEA), cytokeratin-19 fragment (CYFRA21-1) and squamous cell carcinoma antigen (SCC-Ag) in the two groups after treatment decreased, and the experimental group was lower than the control group (P<0.05). After treatment, CD8+ increased in the two groups, but the experimental group was lower than the control group. After treatment, CD3+, CD4+, CD4+ /CD8+ all decreased, but the experimental group was higher than the control group (P<0.05). After treatment, the level of serum Livin in the two groups decreased compared with that before treatment, and the experimental group was lower than the control group (P<0.05), the levels of serum PDCD5, P53 and Bax in the two groups after treatment were higher compared with those before treatment, and the experimental group was higher than the control group (P<0.05). There was no significant difference in the incidence of adverse reactions in the two groups (P>0.05). PFS and OS in the experimental group were higher than those in the control group (P<0.05). Conclusion: Gefitinib combined with PC chemotherapy can regulate the levels of serum apoptosis factors and tumor markers, and effectively improve the immune function and prognosis of patients with EGFR mutation-positive advanced lung adenocarcinoma. [ABSTRACT FROM AUTHOR]

Objective: To investigate clinical efficacy of pediatric massage combined with Xiaoer Xiaoji Zhike oral liquid and azithromycin in the treatment of children of mycoplasma pneumoniae pneumonia (MPP) and its effect on immune function and inflammatory factors. Methods: 80 cases of children with MPP who were treated in our hospital from March 2018 to June 2019 were selected. According to the different treatment methods, they were divided into the control group (azithromycin and Xiaoer Xiaoji Zhike oral liquid treatment, n=40) and the study group (pediatric massage, Xiaoer Xiaoji Zhike oral liquid, azithromycin treatment, n=40). The clinical efficacy of the two groups and the improvement of children's symptoms during the treatment were observed, peripheral blood T lymphocyte subsets, adverse reactions and serum inflammatory factors between the two groups were compared. Results: Compared with the control group, the clinical total effective rate of the study group was higher (P<0.05). Compared with the control group, the study group had a shorter of lung rales, cough and X-ray film of lung lesions to disappear time and antipyretic time (P<0.05). Compared with the control group, CD3+, CD4+ and CD4+/CD8+ in the study group was higher at 14 d after treatment, CD8+ was lower (P<0.05). Compared with the control group, interleukin-1β (IL-1β), C-reactive protein (CRP) and interleukin-6 (IL-6) in the study group were lower (P<0.05). There was no significant difference in incidence of adverse reactions in two groups (P>0.05). Conclusion: Pediatric massage combined with Xiaoer Xiaoji Zhike oral liquid and azithromycin in the treatment of children with MPP can promote the improvement of symptoms, optimize the clinical treatment effect, and effectively regulate the immune function and inflammatory factor levels of children. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effect of berberine on gastric mucosal injury and immune function in mice infected with Helicobacter pylori. Methods: Fifty SPF male mice were randomly divided into 5 groups, including blank control group, Helicobacter pylori model group, berberine low-dose group (25 mg/mL, group A), medium-dose group (50 mg/mL, group B) and high-dose group (100 mg/kg/d, group C). Rats in blank group were intraperitoneally injected with normal saline of equal volume, and the other groups were orally administered Helicobacter pylori to establish a mouse model of Helicobacter pylori infection. The survival rate and apoptosis rate of gastric mucosal epithelial cells were detected, and the levels of apoptotic protein, inflammatory factors, immune function and IL-4/STAT6 mRNA of gastric mucosal epithelial cells were measured. Results: The survival rate of gastric mucosal epithelial cells in model group, berberine low-dose, medium-dose and high-dose groups was significantly lower than that in blank control group (P<0.05). With the increase of berberine dose, cell survival rate was gradually increased (P<0.05). The apoptosis rate of gastric mucosal epithelial cells in model group, berberine low-dose, medium-dose and high-dose groups was significantly higher than that in blank control group (P<0.05). With the increase of berberine dose, the apoptosis rate decreased gradually (P<0.05). Compared with model group, the levels of apoptotic protein Bax and Cl-caspase-3, TNF-α, IFN-γ, IL-10 and TGF-β of mouse gastric mucosal epithelial cells in berberine low, medium and high dose groups were significantly decreased (P<0.05), and with the increase of berberine dose, The levels of Bax and Cl-caspase-3, TNF-α, IFN-γ, IL-10 and TGF-β were decreased (P<0.05). Compared with model group, the levels of CD3+, CD4+ and CD8+, IL-4 mRNA and STAT6 mRNA in the supernatant of berberine low-dose, medium-dose and high-dose groups were significantly increased(P<0.05), and with the increase of berberine dose, CD3+, CD4+ and CD8+ levels, IL-4 mRNA and STAT6 mRNA were increased gradually(P<0.05). Conclusion: Berberine can alleviate gastric mucosal damage and enhance immune function in mice infected with Helicobacter pylori, which may be related to IL-4/STAT6 signaling pathway. [ABSTRACT FROM AUTHOR]

Objective: To observe the effects of cardiopulmonary rehabilitation training combined with acupoint application for treatment of winter disease in summer on pulmonary function, immune function and quality of life of patients with chronic obstructive pulmonary disease (COPD) in stable stage. Methods: 80 patients with COPD in stable stage who were received by the Minda Hospital Affiliated to Hubei Minzu University from March 2021 to March 2022 were included in the study. According to the method of random number table, they were divided into the study group (n=40, receiving cardiopulmonary rehabilitation training combined with acupoint application for treatment of winter disease in summer) and the control group (n=40, receiving cardiopulmonary rehabilitation training). 6minute walk test (6MWT), hospitalization times of COPD acute exacerbation, hospitalization time, pulmonary function, immune function and quality of life were compared in the two groups. Results: The 6MWT in the study group after treatment was longer than that in the control group (P＜0.05), the hospitalization times of COPD acute exacerbation in the study group was less than that in the control group, and the hospitalization time was shorter than that in the control group (P＜0.05). The scores of cough, sleep, sputum, mood, exercise endurance, chest tightness, energy and daily exercise in the study group after treatment were lower than those in the control group (P＜0.05). After treatment, CD4+, CD4+/CD8+, immunoglobulin (Ig) A, IgG and IgM in the study group were higher than those in the control group, and CD8+ was lower than that in the control group (P＜0.05). Forced vital capacity (FVC), peak expiratory flow rate (PEF) and forced expiratory volume in the first second (FEV1) in the study group were higher than those in the control group (P＜0.05). Conclusion: The acupoint application for treatment of winter disease in summer combined with cardiopulmonary rehabilitation training for the treatment of patients with COPD in stable stage can effectively improve the patients’ immune function and lung function, and improve the patients’ quality of life. [ABSTRACT FROM AUTHOR]

Objective: To study the influencing factors of high nutritional risk in children with sepsis and their effects on immune function, trace elements and disease outcome. Methods: 168 children with sepsis who were admitted to our hospital from January 2019 to December 2021 were selected, the nutritional status and growth risk screening tool (STRONGkids) was used to screen their nutritional risk, according to the scoring results, the children with nutritional risk were divided into medium-low nutritional risk group and high nutritional risk group. The clinical data of the two groups were compared, and the influencing factors of high nutritional risk were analyzed by Logistic regression model. The indexes of immune function (CD4+, CD8+, CD4+/CD8+), the contents of trace elements [copper (Cu), iron (Fe), zinc (Zn)] and the disease outcome situation were compared between the low and middle nutrition risk group and the high nutrition risk group. Results: The results of STRONGkids nutritional risk screening showed that 151 children with sepsis had nutritional risk, and the incidence of nutritional risk was 89.88%, including 110 cases of medium-low nutritional risk (medium-low nutritional risk group) and 41 cases of high nutritional risk (high nutritional risk group). The acute physiology and chronic health score system II (APACHE II) score, lactic acid, procalcitonin levels and the proportion of severe sepsis in the high nutritional risk group were higher than those in the medium-low nutritional risk group, and the albumin level was lower than that in the medium-low nutritional risk group (P<0.05). Multivariate Logistic regression analysis showed that increased APACHE II score and severe sepsis were risk factors for high nutritional risk in children with sepsis (P<0.05), and increased albumin was a protective factor for high nutritional risk in children with sepsis (P<0.05). CD4+ and CD4+/CD8+ in the high nutritional risk group were lower than those in the medium-low nutritional risk group, and CD8+ was higher than that in the medium-low nutritional risk group (P＜0.05). There was no significant difference in Cu content between the two groups (P>0.05). The Fe and Zn contents in the high nutritional risk group were lower than those in the medium-low nutritional risk group (P＜0.05). There were 74 cases of improvement, 32 cases of unrecovery, and 4 cases of death in the medium-low nutritional risk group, while 19 cases of improvement, 15 cases of unrecovery and 7 cases of death in the high nutritional risk group, the good disease outcome rate in the high nutritional risk group was lower than that in the medium-low nutritional risk group (P＜0.05). Conclusion: The incidence of high nutritional risk is high in children with sepsis. Severe sepsis, APACHE Ⅱ score and albumin level are the influencing factors of high nutritional risk, and high nutritional risk leads to the decline of immune function, the reduction of Fe and Zn content in the body, and poor disease outcome. Early nutritional intervention is recommended to improve the prognosis of children. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of Feilike Heji combined with budesonide and compound ipratropium bromide on lung function, inflammatory mediators and immune function in children with bronchial asthma. Methods: 76 children with bronchial asthma who were received by the pediatric department of our hospital from February 2021 to March 2022 were selected as the observation objects. The children were divided into the control group (n=38, budesonide and compound ipratropium bromide treatment) and the study group (n=38, Feilike Heji combined with budesonide and compound ipratropium bromide treatment) by envelope lottery. The curative effect, lung function, inflammatory mediators and immune function of the two groups were compared, at the same time, the drug safety status of the two groups during treatment was recorded. Results: The total clinical effective rate in the study group was significantly higher than that in the control group (P<0.05). 2 weeks after treatment, forced expiratory volume in the first second (FEV1), forced expiratory flow (PEF) and forced vital capacity (FVC) in the study group were higher than those in the control group (P<0.05). 2 weeks after treatment, interleukin-6 (IL-6), tumor necrosis factor-α (TNF-α) and C-reactive protein (CRP) in the study group were lower than those in the control group, and interferon-γ (IFN-γ) was higher than that in the control group (P<0.05). 2 weeks after treatment,CD8+in the study group was lower than that in the control group, and CD3+, CD4+, CD4+/CD8+ were higher than those in the control group (P<0.05). There was no significant difference in the incidence of adverse reactions between groups (P>0.05). Conclusion: Feilike Heji combined with budesonide and compound ipratropium bromide in the treatment of children with bronchial asthma can effectively improve the lung function and immune function of children, reduce the level of inflammatory mediators, and which has a reliable curative effect. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of Jiawei Bazhen decoction combined with m FOLFOX6 regimen on tumor markers, immune function and peripheral blood phosphatidylinositol3-ki-nase (PI3K) -serine protein kinase (Akt) signal pathway in patients with advanced colorectal cancer. Methods: According to the random number table method, 96 patients with advanced colorectal cancer who were treated in Beibei Hospital of Traditional Chinese Medicine Affiliated to Guangzhou University of Traditional Chinese Medicine from September 2020 to January 2022 were divided into control group (48 cases, treated with m FOLFOX6 regimen) and study group (48 cases, treated with Jiawei Bazhen decoction combined with m FOLFOX6 regimen) . The efficacy, TCM syndrome score, Karnofsky score (KPS), tumor markers, immune function and peripheral blood PI3K-Akt signal pathway related indexes were compared between the two groups. Results: Compared with the control group, the total clinical effective rate in the study group was higher (P＜0.05). After treatment, The total of TCM syndrome score in the study group after treatment was lower than that in the control group, and the KPS score was higher than that in the control group (P＜0.05). After treatment, CD4+, CD4+/CD8+, immunoglobulin (Ig) M and Ig A in the study group were higher than those in the control group, and CD8+was lower than that in the control group (P＜0.05). After treatment, the serum carcinoembryonic antigen (CEA), carbohydrate antigen 199 (CA199) and carbohydrate antigen 72-4 (CA72-4) in the study group were lower than those in the control group (P＜0.05). After treatment, the expression levels of PI3K, Akt m RNA in the peripheral blood of study group were lower than those in the control group (P＜0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P＞0.05). Conclusion: Jiawei Bazhen decoction combined with m FOLFOX6 regimen has a significant effect in the treatment of advanced colorectal cancer, which can effectively prevent the disease progression of advanced colorectal cancer, enhance immunity, and reduce the level of serum tumor markers. Its therapeutic mechanism may be related to the regulation of peripheral blood PI3K-Akt signal pathway. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of Kanglaite injection combined with gemcitabine and cisplatin (GP) regimen on immune function, angiogenesis and serum Janus kinase 2 (JAK2) / signal transducer and activator of transcription 3 (STAT3) signal pathway in patients with advanced non-small cell lung cancer (NSCLC). Methods: 80 patients with advanced NSCLC admitted to the Affiliated Hospital of Hunan Academy of Traditional Chinese Medicine from February 2019 to February 2020 were selected.According to the random number table method, they were divided into control group (GP chemotherapy, n=40) and observation group (Kanglaite injection combined with GP chemotherapy, n=40). After treatment, the efficacy, immune function, angiogenesis indexes and JAK2/STAT3 signal pathway related indexes changes of the two groups were observed, and the incidence of adverse reactions was recorded, the patients were followed up for 2 years to observe the survival and prognosis. Results: The total clinical effective rates of the control group and the observation group were 60.00% (24/40) and 82.50% (33/40) respectively, there was significant difference between the two groups (P>0.05). Compared with the control group, CD8+ was lower, CD3+, CD4+ and CD4+/CD8+ were higher in the observation group after treatment (P<0.05). Compared with the control group, vascular endothelial growth factor (VEGF) was further decreased, and tissue inhibitor of metalloproteinase-2 (TIMP-2) was further increased in the observation group after treatment (P<0.05). Compared with the control group, JAK2mRNA and STAT3mRNA in the observation group after treatment were further decreased (P<0.05). There was no statistical difference in the incidence of adverse reactions between the two groups (P>0.05). The median survival of the observation group was 19 months, which was significantly longer than 10 months of the control group, and the difference was statistically significant (P<0.05). Conclusion: Kanglaite injection combined with GP regimen for patients with advanced NSCLC can prevent disease progression to a certain extent, alleviate immunosuppression and prolong survival, which may be related to down-regulation of JAK2/STAT3 signaling pathway. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of gestational diabetes mellitus(GDM) on neonatal cardiac development, immune function and cord blood bilirubin. Methods: The clinical data of 80 neonates of GDM patients (GDM group) and 40 neonates (control group) of healthy parturients delivered in our hospital from July 2019 to July 2021 were retrospectively analyzed. The differences of general indexes, cardiac development indexes, immune function indexes and umbilical cord blood bilirubin levels between the two groups were compared. Results: The birth weight of neonatal in GDM group was higher than that in control group (P<0.05). There were no differences in body length, gender and gestational age between the two groups (P>0.05). The aorta/main pulmonary artery width, the size of left atrium, left/right ventricle and the ventricular septum thickness in GDM group neonatal were greater than those in control group (P<0.05). There were no differences in left ventricular short axis contraction rate and ejection fraction between the two groups (P>0.05). The serum immunoglobulin (Ig)G, CD3, CD4 and CD4/CD8 levels in GDM group neonatal were lower than those in control group, and the CD8 level was higher than that in control group (P<0.05). There were no significant differences in serum IgM and IgA levels between the two groups (P>0.05). The cord blood bilirubin level in GDM group neonatal was (41.25±8.06)μmol/L, which was higher than (33.36±7.52)μmol/L in the control group neonatal, the difference was statistically significant (P<0.05). Conclusion: GDM has adverse effects on neonatal heart development, which can lead to myocardial hypertrophy, significantly reduce the immune function of GDM neonatal, and increased neonatal cord blood bilirubin level. [ABSTRACT FROM AUTHOR]

Objective: To observe the clinical curative effect of anti-tuberculosis drugs assisted with spleen aminopeptide on tuber- culosis. Methods: According to random number table method, 120 patients with tuberculosis admitted to the hospital were divided into observation group and control group between June 2016 and June 2021. The control group was treated with routine anti-tuberculosis drugs, while observation group was additionally treated with spleen aminopeptide. The changes of lung lesions and cavities in both groups were evaluated. The negative conversion ratios of sputum culture after 2, 4 and 6 months of treatment, peripheral blood T lympho- cyte subsets (CD3+, CD4+, CD4+ /CD8+ ratio) before and after treatment in both groups were detected. Results: The total response rates of lung lesions and lung cavities in observation group were 95.00% and 93.33%, significantly higher than those in control group (83.33%, 80.00%) (P<0.05). After 6 months of treatment, difference in negative conversion ratio of sputum culture between the two groups was not statistically significant (P>0.05). After 2 and 4 months of treatment, negative conversion ratios of sputum culture in observation group were significantly higher than those in control group (P<0.05). The difference in CD3+, CD4+ and CD4+ /CD8+ ratio between the two groups before treatment, as well as those in control group before and after treatment was not significantly different ( P>0.05). After treat- ment, CD3+, CD4+ and CD4+ /CD8 + ratio in observation group were significantly increased, significantly higher than those in control group (P<0.05). Conclusions: The anti-tuberculosis drugs assisted with spleen aminopeptide are beneficial to improve clinical curative effect on tuberculosis, which may be related to enhancing immune function. And it is worthy of further clinical research. [ABSTRACT FROM AUTHOR]

Objective: To explore and analysis the regulatory effect of sodium ferulate on the immune function and phagocytic function of alveolar macrophages in pulmonary tuberculosis model mice. Methods: Pulmonary tuberculosis model mice (n=36) were randomly divided into model group, rifampicin group and sodium ferulate group, with 12 mice in each group. Rifampicin group and sodium ferulate group were given 100 mg/kg dose of rifampicin and sodium ferulate respectively, and the mice in the model group were given the same amount of normal saline, once a day, for 6 weeks, and the general characteristics of all mice were observed and recorded; the pathological characteristics of the mice were observed by HE staining at the 2nd, 4th, and 6th weeks of administration; MDA detection kit and total SOD activity detection kit were used to determine the level of MDA and SOD activity in lung tissue; Flow cytometry was used to detect the levels of mouse T lymphocyte subsets-CD3+T lymphocytes and CD4+T lymphocytes; Serum IL-6 and IL-8 levels were detected by enzyme-linked immunosorbent assay; The apoptosis rate of alveolar macrophages was detected by AnnexinV-FITC. Results: The levels of MDA in the lung tissue of the rifampicin group and the sodium ferulate group were lower than those of the model group (P<0.05), and the SOD activity were higher than that of the model group (P<0.05) in the 2nd, 4th, and 6th weeks after administration, and there were also significant difference between the rifampicin group and the sodium ferulate group (P<0.05). The ratios of blood CD3+T lymphocytes and CD4+T lymphocytes in the rifampicin group and sodium ferulate group were significantly higher than those in the model group in the 2nd, 4th, and 6th weeks of administration (P<0.05). The sodium group were also higher than the rifampicin group (P<0.05). The serum levels of IL-6 and IL-8 in the rifampicin group and the sodium ferulate group were significantly lower than those in the model group in the 2nd, 4th, and 6th weeks after administration (P<0.05), and the sodium fululate group were also lower than the model group. in the rifampicin group (P<0.05). The apoptosis rate of macrophages in BALF in the rifampicin group and sodium ferulate group at the 2nd, 4th, and 6th weeks of administration were significantly lower than that in the model group (P<0.05). the sodium ferulate group were also significantly lower than the rifampicin group (P<0.05). Conclusion: The application of sodium ferulate in pulmonary tuberculosis model mice can inhibit the expression of inflammatory factors, improve the oxidation status, enhance the immune function of mice, and reduce the apoptosis rate of macrophages in BALF. [ABSTRACT FROM AUTHOR]

Objective: To observe the value of electroacupuncture nerve stimulation therapy combined with pelvic floor muscle training in the rehabilitation of early cervical cancer after extensive total hysterectomy. Methods: 98 patients with early cervical cancer undergoing extensive total hysterectomy who were admitted to our hospital from March 2020 to October 2021 were prospectively collected. They were divided into study group and control group by random number table method. The control group was instructed to conduct pelvic floor muscle training, and the study group was given electroacupuncture nerve stimulation therapy on the basis of pelvic floor muscle training. The incidence of urinary retention and the recovery of bladder function, immune function and pelvic floor function were compared between the two groups. Results: The results of 13 weeks after operation showed that the normal rate of comprehensive muscle strength of pelvic floor muscle in the study group was higher than that in the control group (P<0.05). Initial urinary bladder volume (VS), maximum urinary flow rate (MFR) and maximum urinary bladder volume (MVS) in two groups at 13 weeks after operation were higher than those at 1 week after operation, while residual urine volume (PVR) was lower than that at 1 week after operation. FVS, MFR and MVS in the study group at 13 weeks after operation were higher than those in the control group, while PVR was lower than that in the control group (P<0.05). The levels of serum immunoglobulin A (Ig A), immunoglobulin G (Ig G),immunoglobulin M (Ig M), peripheral blood T lymphocyte CD3+, CD4+/CD8+in the two groups at 13 weeks after operation were higher than those at 1 week after operation, and the study group was higher than the control group (P<0.05). The incidence of urinary retention in the study group was 4.08% (2/49) lower than 22.45% (11/49) in the control group, and the difference was statistically significant (P<0.05). Conclusion: Electroacupuncture nerve stimulation therapy combined with pelvic floor muscle training can reduce the incidence of urinary retention in patients with early cervical cancer after extensive total hysterectomy, improve pelvic floor function and bladder function, and promote the recovery of immune function, and with good effect. [ABSTRACT FROM AUTHOR]

Objective: To explore the clinical observation of shoulder joint exercise eight type rehabilitation exercise combined with Taijiquan in patients with modified radical mastectomy for breast cancer. Methods: From January 2020 to December 2021, 96patients with modified radical mastectomy for breast cancer were selected and divided into two groups according to the random number table. They were 48 cases in the control group and treated with eight exercises in shoulder joint exercise. 48 cases in the observation group and treated with eight type rehabilitation exercise of shoulder joint exercise combined with Taijiquan exercise. Functional recovery of affected limb, sleep quality, changes of immune function and incidence of lymphedema were compared between the two groups.Results: 12 weeks after intervention, range of motion(ROM) of affected limbs in the two groups were expanded, and the observation group was larger than the control group (P<0.05). 12 weeks after intervention, scores of Pittsburgh Sleep Quality Index(PSQI) items in the two groups decreased, and the observation group was lower than the control group (P<0.05). 12 weeks after intervention, CD3+, CD4+,CD4+/CD8+ increased in the two groups, and the observation group was higher than the control group, while CD8+ decreased in the two groups, and the observation group was lower than the control group(P<0.05). The incidence of lymphedema in observation group was lower than that in control group (P<0.05). Conclusion: Based on the eight types of shoulder joint exercises, combined with Taijiquan exercise, the patients with modified radical mastectomy for breast cancer can effectively improve sleep quality and immune function,promote recovery of limb function, and reduce the incidence of lymphedema, which has good effect. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of Duyiwei capsule on immune function and the expression of nuclear factor-κB（NF-κB） inflammatory pathway in recurrent oral ulcer (ROU) rats. Methods: 60 SD rats were randomly divided into normal group, model group and Duyiwei capsule low, medium and high dose group, 12 rats in each group. Except the normal group, the ROU model was established by immunoassay in other groups. 8 weeks after modeling, the low, medium and high dose groups of Duyiwei capsule were treated with 0.01mg/mL, 0.02 mg/mL and 0.03 mg/mL Duyiwei capsule solution by gavage respectively. The normal group and model group were treated with the same amount of normal saline by gavage, they were treated continuously for 20d. The number of oral ulcers, duration time and area of oral ulcers of rats in each group were recorded. Serum interleukin (IL）-1β, IL-6 and tumor necrosis factor-α（TNF-α） levels were measured by enzyme-linked immunosorbent assay, peripheral blood CD3+, CD4+ and CD8+ percentages were measured by flow cytometry and CD4+/CD 8+ ratio was calculated. NF-κB p65, phosphorylation inhibitor of NF-κB α（p-IκBα） and IkappaB kinase α（IKKα） protein levels in oral mucosa tissues were measured by immunoblotting. Results: No oral ulcers appeared in the normal group, the number of ulcers decreased, the duration time shortened and the area of ulcers decreased in the model group, the Duyiwei capsule low dose group, the Duyiwei capsule medium dose group and the Duyiwei capsule high dose group in that order (P<0.05）. The IL-1β, IL-6, TNF-α, CD8+, NF-κB p65, p-IκBα and IKKα levels decreased in the model group, the Duyiwei capsule low dose group, the Duyiwei capsule medium dose group, the Duyiwei capsule high dose group and the normal group in that order, and the CD3+, CD4+and CD4+/CD8+increased in that order (P<0.05）. Conclusion: Duyiwei capsule can significantly reduce the number and area of oral ulcers in Rou rats, shorten the healing time, and the effect is dose-dependent. Its mechanism may be related to improving immune function and inhibiting NF-κB inflammatory pathway. [ABSTRACT FROM AUTHOR]

Objective: To investigate the effects of sufentanil combined with dezocine controlled analgesia on stress response, immune function and inflammatory factors in patients after abdominal hysterectomy. Methods: 78 cases of patients who underwent abdominal hysterectomy in our hospital from April 2020 to August 2021 were selected. The patients were divided into control group (received sufentanil controlled analgesia) and observation group ( sufentanil combined with dizocine controlled analgesia) according to the random number table method, with 39 cases in each group. The sedation and analgesia situation [visual pain simulation (VAS) score and Ramsay sedation score], stress response [norepinephrine (NE), epinephrine (E) and cortisol (Cor)], immune function [CD3+, CD4+, CD8+, calculate CD4+/CD8+] and inflammatory factors [interleukin-2 (IL-2), interleukin-6 (IL-6), C-reactive protein (CRP), tumor necrosis factor-α (TNF-α)] were observed and compared between the two groups. The safety of the two anesthesia regimens was recorded at the same time. Results: The VAS scores of the two groups increased first and then decreased at 6 h, 24 h and 48 h after operation, and the VAS scores of the observation group at 24 h and 48 h after operation were lower than those of the control group at the same time point (P<0.05). There was no significant difference between the two groups at different time points of Ramsay Sedation score at 6 h, 24 h and 48 h after operation (P>0.05). The levels of serum NE, E and Cor in the two groups increased at 48 h after analgesia, but the levels of serum NE, E and Cor of the observation group were lower than those of the control group at the same time point (P<0.05). The levels of serum IL-2, IL-6, CRP and TNF-琢in the two groups at 48 h after analgesia increased, but the levels of serum IL-2, IL-6, CRP and TNF-琢of the observation group at 48 h after analgesia were lower than those of the control group at the same time point (P<0.05). 48 h after analgesia, CD8+ increased, and CD3+, CD4+, CD4+/CD8+ decreased in the two groups, but the change range of the observation group was less than that of the control group(P<0.05). There was no significant difference in the incidence of adverse reactions between the two groups (P>0. 05). Sufentanil combined with dizocine for patient-controlled analgesia after transabdominal hysterectomy has significant sedative and analgesic effects. At the same time, it can also reduce inflammatory injury and stress reaction and promote the recovery of immune function. [ABSTRACT FROM AUTHOR]

Objective To explore the effects of Matrix metalloproteinases (MMPs) inhibitors on apoptosis, immune function and inflammatory factors of colon cancer cells. Methods SW480 colon cancer cells were randomly divided into low expression group (mixed culture of MMPs inhibitor lentivirus plasmid and SW480 cells) and blank control group (mixed culture of SW480 cells and lentivirus packaging plasmid). Flow cytometry, immunocytochemistry, Enzyme- Linked immunosorbent assay were used. SW480 cell apoptosis was detected by ELISA, real-time fluorescence quantitative PCR (QRT-PCR) and Hoechst 33258 fluorescence staining. TNF-琢, IL-6 protein expression, immunoglobulin expression level, MMP-9 mRNA expression level. Results Compared with the blank control group and the low expression group, the expression level of MMP-9 mRNA in SW480 cells in the low expression group was significantly decreased (P<0.05), indicating that the transfection was successful. Compared with blank control group, the number of apoptosis of SW480 cells in low expression group was significantly increased (P<0.05). The probability of nuclear pyknosis in low expression group was significantly higher than that in blank control group (P<0.05). Compared with blank control group, the expression level of IL-2 in SW480 cells in low expression group was significantly increased (P<0.05), and the expression levels of IL-4, TGF-茁1 and TIM-1 in SW480 cells were significantly decreased (P<0.05). There was a negative correlation between MMP-9 mRNA and IL-2 in SW480 cells (r=-0.723, P=0. 007), MMP-9 mRNA and IL-4, TGF- 茁1 and TIM-1 showed negative correlation (all P<0.05). The positive number of TNF-琢and IL-6 protein in SW480 cells in the blank control group was the highest, and the positive number of TNF-琢and IL-6 protein in SW480 cells in the low expression group was the lowest. Compared with the blank control group, the positive number of TNF-琢and IL-6 protein in SW480 cells in the low expression group was significantly decreased (all P<0.05). Conclusion MMPs inhibitors can promote apoptosis of SW480 cells, improve immune function, and reduce the expression level of inflammatory mediators. [ABSTRACT FROM AUTHOR]