木霉菌醇通过 p53 信号通路对胃癌大鼠的肠道微生态、胃肠道功能 及免疫功能的影响.

Objective: Effects of Trichoderma on intestinal microecology, gastrointestinal function and immune function of gastric cancer rats through p53 signaling pathway. Methods: 40 male SD rats were randomly divided into model group, low dose group, middle dose group and high dose group, 10 rats in each group. Another 10 healthy male rats were selected and divided into blank group. After successful modeling, the blank group and the model group were given the same amount of normal saline by gavage, and the low, medium and high dose groups of trichoderma alcohol were given 20, 40 and 60 mg / kg by gavage. The expression of p53 protein was detected by Western blot. The changes of intestinal flora were detected by 16 S rDNA assay. The levels of serum gastrointestinal function indexes were detected by enzyme-linked immunosorbent assay (ELISA). Peripheral blood T lymphocyte subsets were detected by flow cytometry. Results: The expression level of p53 protein in the model group and the low, medium and high dose groups was lower than that in the blank group, and the p53 protein level in the medium dose group was higher than that in the model group and the low and high dose groups (P<0.05). The levels of Firmicutes, Bacteroidetes and Proteobacteria in the model group and Trichoderma alcohol (low, medium and high dose) groups were significantly higher than those in the blank group. The levels of Firmicutes, Bacteroidetes and Proteobacteria in the medium dose group were significantly lower than those in the model group and Trichoderma alcohol (low and high dose) groups (P<0.05). The levels of gastrin and motilin in the model group and the trichoderma alcohol (low, medium and high dose) group were significantly lower than those in the blank group, and the levels of gastrin (GAS) and motilin (MTL) in the middle and low dose group of trichoderma alcohol were significantly higher than those in the model group and the trichoderma alcohol (low and high dose) group (P<0. 05). Compared with the blank group, the levels of CD3+ and CD4+ in the model group and the Trichoderma alcohol (low, medium and high dose) groups were higher, and the level of CD8+ was lower. Compared with the model group and the low-dose and high-dose groups, the levels of CD3+ and CD4+ in the low-dose group were lower, and the level of CD8+ was higher (P<0.05). Conclusion: Trichoderma alcohol can effectively regulate the intestinal flora of gastric cancer rats, enhance their immune function, and promote the recovery of intestinal function, and the medium dose application effect is better. [ABSTRACT FROM AUTHOR]