1. Selective Human Serum Albumin Sensor from the Screening of a Fluorescent Rosamine Library
- Author
-
Young Hoon Ahn, Jun-Seok Lee, and Young-Tae Chang
- Subjects
Serum albumin ,Biosensing Techniques ,Sensitivity and Specificity ,Fluorescence ,Small Molecule Libraries ,Species Specificity ,medicine ,Animals ,Combinatorial Chemistry Techniques ,Humans ,Serum Albumin ,Fluorescent Dyes ,Chromatography ,Molecular Structure ,biology ,Chemistry ,Extramural ,Serum Albumin, Bovine ,Stereoisomerism ,General Chemistry ,Human serum albumin ,Highly selective ,Highly sensitive ,body regions ,Fluorescence intensity ,Biochemistry ,biology.protein ,Cattle ,medicine.drug ,Macromolecule - Abstract
A fluorescent dye library approach for the development of a bioanalyte sensor was sought. The screening of a rosamine dye library against diverse macromolecules led to the discovery of a highly sensitive human serum albumin binder, G13, with approximately 36-fold fluorescence intensity change. G13 showed a highly selective response to HSA over other macromolecules including albumins from other species. The potential use of G13 for the detection of HSA in biofluids is described.
- Published
- 2008