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Rapid Identification of Immunostimulatory α-Galactosylceramides Using Synthetic Combinatorial Libraries
- Source :
- Journal of Combinatorial Chemistry. 9:1084-1093
- Publication Year :
- 2007
- Publisher :
- American Chemical Society (ACS), 2007.
-
Abstract
- Two 60+-membered libraries of alpha-galactosylceramides have been prepared by reactions between activated ester resins and two core, fully deprotected galactosylated sphingoid bases. The libraries were evaluated for their ability to stimulate CD1d-restricted NKT cells, using in vitro stimulation of a murine NKT cell hybridoma line and for their ability to induce the expansion of NKT cells from peripheral blood mononuclear cells (PBMC) of a normal human subject. Our results showed that many compounds constructed on a C18-phytosphingosine base had significant stimulatory activity in both assays. Because no product purification was required, this approach is particularly attractive as a method for rapid synthesis of large libraries of potential immunomodulatory glycosylceramides.
- Subjects :
- Galactosylceramides
CD1
Alpha (ethology)
chemical and pharmacologic phenomena
Lymphocyte Activation
Peripheral blood mononuclear cell
In vitro stimulation
Mice
Antigen
Sphingosine
Animals
Combinatorial Chemistry Techniques
Humans
Sphingolipids
Chemistry
Galactose
hemic and immune systems
General Chemistry
Natural killer T cell
Combinatorial chemistry
Killer Cells, Natural
Rapid identification
Resins, Synthetic
Models, Chemical
Biochemistry
Leukocytes, Mononuclear
Subjects
Details
- ISSN :
- 15204774 and 15204766
- Volume :
- 9
- Database :
- OpenAIRE
- Journal :
- Journal of Combinatorial Chemistry
- Accession number :
- edsair.doi.dedup.....3390cdbd17ec02cfcf3c68d037045b58