123 results on '"Sandra Lia do Amaral"'
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2. Diretrizes Brasileiras de Hipertensão Arterial – 2020
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Weimar Kunz Sebba Barroso, Cibele Isaac Saad Rodrigues, Luiz Aparecido Bortolotto, Marco Antônio Mota-Gomes, Andréa Araujo Brandão, Audes Diógenes de Magalhães Feitosa, Carlos Alberto Machado, Carlos Eduardo Poli-de-Figueiredo, Celso Amodeo, Décio Mion Júnior, Eduardo Costa Duarte Barbosa, Fernando Nobre, Isabel Cristina Britto Guimarães, José Fernando Vilela-Martin, Juan Carlos Yugar-Toledo, Maria Eliane Campos Magalhães, Mário Fritsch Toros Neves, Paulo César Brandão Veiga Jardim, Roberto Dischinger Miranda, Rui Manuel dos Santos Póvoa, Sandra C Fuchs, Alexandre Alessi, Alexandre Jorge Gomes de Lucena, Alvaro Avezum, Ana Luiza Lima Sousa, Andrea Pio-Abreu, Andrei Carvalho Sposito, Angela Maria Geraldo Pierin, Annelise Machado Gomes de Paiva, Antonio Carlos de Souza Spinelli, Armando da Rocha Nogueira, Nelson Dinamarco, Bruna Eibel, Cláudia Lúcia de Moraes Forjaz, Claudia Regina de Oliveira Zanini, Cristiane Bueno de Souza, Dilma do Socorro Moraes de Souza, Eduardo Augusto Fernandes Nilson, Elisa Franco de Assis Costa, Elizabete Viana de Freitas, Elizabeth da Rosa Duarte, Elizabeth Silaid Muxfeldt, Emilton Lima Júnior, Erika Maria Gonçalves Campana, Evandro José Cesarino, Fabiana Marques, Fábio Argenta, Fernanda Marciano Consolim-Colombo, Fernanda Spadotto Baptista, Fernando Antonio de Almeida, Flávio Antonio de Oliveira Borelli, Flávio Danni Fuchs, Frida Liane Plavnik, Gil Fernando Salles, Gilson Soares Feitosa, Giovanio Vieira da Silva, Grazia Maria Guerra, Heitor Moreno Júnior, Helius Carlos Finimundi, Isabela de Carlos Back, João Bosco de Oliveira Filho, João Roberto Gemelli, José Geraldo Mill, José Marcio Ribeiro, Leda A. Daud Lotaif, Lilian Soares da Costa, Lucélia Batista Neves Cunha Magalhães, Luciano Ferreira Drager, Luis Cuadrado Martin, Luiz César Nazário Scala, Madson Q. Almeida, Marcia Maria Godoy Gowdak, Marcia Regina Simas Torres Klein, Marcus Vinícius Bolívar Malachias, Maria Cristina Caetano Kuschnir, Maria Eliete Pinheiro, Mario Henrique Elesbão de Borba, Osni Moreira Filho, Oswaldo Passarelli Júnior, Otavio Rizzi Coelho, Priscila Valverde de Oliveira Vitorino, Renault Mattos Ribeiro Junior, Roberto Esporcatte, Roberto Franco, Rodrigo Pedrosa, Rogerio Andrade Mulinari, Rogério Baumgratz de Paula, Rogério Toshiro Passos Okawa, Ronaldo Fernandes Rosa, Sandra Lia do Amaral, Sebastião R. Ferreira-Filho, Sergio Emanuel Kaiser, Thiago de Souza Veiga Jardim, Vanildo Guimarães, Vera H. Koch, Wille Oigman, and Wilson Nadruz
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Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Published
- 2021
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3. Photobiomodulation by Led Does Not Alter Muscle Recovery Indicators and Presents Similar Outcomes to Cold-Water Immersion and Active Recovery
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Elvis de Souza Malta, Fabio Santos de Lira, Fabiana Andrade Machado, Anderson Saranz Zago, Sandra Lia do Amaral, and Alessandro Moura Zagatto
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low-level light therapy ,high-intensity interval training ,inflammation ,interleukin-10 ,tumor necrosis factor-alpha ,creatine kinase ,Physiology ,QP1-981 - Abstract
Purpose: The aim of the present study was to investigate the effectiveness of photobiomodulation therapy (PBMT) on muscle recovery based on inflammation (interleukin-10 [IL-10]; tumor necrosis factor-α [TNFα]), muscle damage markers (creatine kinase [CK]; lactate dehydrogenase [LDH]), delay onset muscle soreness (DOMS), and countermovement jump performance (CMJ) after two sprint interval training (SIT) sessions compared with a placebo condition (part-I), as well as to compare the effectiveness of PBMT with active recovery (AR) and cold-water immersion (CWI) (part-II).Methods: Part-I was conducted as a double-blind, randomized and placebo-controlled study and part-II as a parallel-group study. Thirty-six men participated in the studies (12 participants in part-I and 36 participants in part-II). Volunteers performed two SITs interspaced by 24-h (SIT1 and SIT2) to mimic the effect of accumulating 2 consecutive days of SIT. In part-I, only after SIT2, PBMT [Total energy: 600J (300J per leg in 5 spots); wavelength: 660–850 nm] or placebo interventions were performed, while in part-II PBMT (part-I data), AR (15-min; 50% of the maximal aerobic power), or CWI (10-min; 10°C) were carried out, also after SIT2. Blood samples were collected before (i.e., baseline), and 0.5, 1, 24, 48, and 72-h after SIT2, while CMJ and DOMS were measured before, 24, 48, and 72-h after SIT2.Results: In part-I, there were no interactions between PBMT and placebo conditions for any blood markers (P ≥ 0.313), DOMS (P = 0.052), and CMJ (P = 0.295). However, an effect of time was found with increases in LDH, CK, and IL-10 (P ≤ 0.043) as well as a decrease in DOMS at 72-h compared with 24-h (P = 0.012). In part-II, there were no interactions between the PBMT, AR, and CWI groups for any markers at the same moments (P ≥ 0.189) and for the peak and integral values (P ≥ 0.193), for DOMS (P = 0.314) and CMJ (P = 0.264). However, an effect of time was found with an increase in CK and IL-10 (P = 0.003), while DOMS decreased at 48 and 72-h compared with 24-h (P = 0.001).Conclusion: In summary, PBMT had no effect on inflammation, muscle damage, CMJ performance, or DOMS after two consecutive sprint interval training sessions compared to placebo, CWI, and AR strategies.
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- 2019
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4. Treatment of Dyslipidemia with Statins and Physical Exercises: Recent Findings of Skeletal Muscle Responses
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Mariana Rotta Bonfim, Acary Souza Bulle Oliveira, Sandra Lia do Amaral, and Henrique Luiz Monteiro
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Dislipidemias / terapia ,Inibidores de Hidroximetilglutaril-CoA Redutases ,Exercício ,Músculo Esquelético ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
Statin treatment in association with physical exercise practice can substantially reduce cardiovascular mortality risk of dyslipidemic individuals, but this practice is associated with myopathic event exacerbation. This study aimed to present the most recent results of specific literature about the effects of statins and its association with physical exercise on skeletal musculature. Thus, a literature review was performed using PubMed and SciELO databases, through the combination of the keywords “statin” AND “exercise” AND “muscle”, restricting the selection to original studies published between January 1990 and November 2013. Sixteen studies evaluating the effects of statins in association with acute or chronic exercises on skeletal muscle were analyzed. Study results indicate that athletes using statins can experience deleterious effects on skeletal muscle, as the exacerbation of skeletal muscle injuries are more frequent with intense training or acute eccentric and strenuous exercises. Moderate physical training, in turn, when associated to statins does not increase creatine kinase levels or pain reports, but improves muscle and metabolic functions as a consequence of training. Therefore, it is suggested that dyslipidemic patients undergoing statin treatment should be exposed to moderate aerobic training in combination to resistance exercises three times a week, and the provision of physical training prior to drug administration is desirable, whenever possible.
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- 2015
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5. Adherence to statin treatment and associated factors in female users from the Unified Health System (SUS)
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Mariana Rotta Bonfim, Arina Hansen, Bruna Camilo Turi, Gabriel de Souza Zanini, Acary Souza Bulle Oliveira, Sandra Lia do Amaral, and Henrique Luiz Monteiro
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Inhibidores de Hidroximetilglutaril-CoA ,Reductasas ,Dislipidemias ,Perfil de salud ,Cumplimiento de la medicación ,Salud pública ,Public aspects of medicine ,RA1-1270 ,Nursing ,RT1-120 ,Mental healing ,RZ400-408 ,Education (General) ,L7-991 - Abstract
Objective: To identify the adherence rate of a statin treatment and possible related factors in female users from the Unified Health System. Method: Seventy-one women were evaluated (64.2 ± 11.0 years) regarding the socio-economic level, comorbidities, current medications, level of physical activity, self-report of muscular pain, adherence to the medical prescription, body composition and biochemical profile. The data were analyzed as frequencies, Chi-Squared test, and Mann Whitney test (p
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- 2014
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6. Presença de fatores de risco de doenças cardiovasculares e de lesões em praticantes de corrida de rua Presence of cardiovascular risck factors and injuries in road runners
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Jaqueline de Castro Ishida, Bruna Camilo Turi, Márcio Pereira-da-Silva, and Sandra Lia do Amaral
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Pedestrianismo ,Saúde ,Hipertensão ,Lesões ,Pedestrianism ,Health ,Hypertension ,Injuries ,Sports ,GV557-1198.995 - Abstract
Este estudo descreveu as características antropométricas e presença de fatores de riscos cardiovasculares (Parte1) bem como a ocorrência de lesões (Parte 2) em corredores de rua. Índice de massa corporal, pressão arterial (PA), circunferência abdominal (CA) e presença de lesões foram avaliadas em 94 corredores. Destes, 38,5% eram hipertensos auto-referidos, mas 41,9% estavam com a PA alterada no dia. Dentre os auto-referidos sem problemas de saúde, foram encontradas alterações na PA (42%), na CA (9,8%) e 6% apresentaram risco cardiovascular moderado. Verificou-se que 34% já sofreram lesão, sendo que 67,7% foram derivadas de treinos ou competições. Idade, distância da prova e realização de outras atividades foram associados com a ocorrência de lesão. Os resultados do presente estudo nos permitem concluir que os praticantes de pedestrianismo apresentam uma condição física propícia ao comprometimento cardiovascular durante uma prova e ao surgimento de lesões, sugerindo campanhas de conscientização sobre a condição de saúde neste público.This study investigated the anthropometric characteristics and the presence of cardiovascular risk factors (Part 1) as well as occurrence of injuries (Part 2) in road runners. Body mass index, blood pressure (BP), abdominal circumference (AC) and incidence of injuries were evaluated in 94 road runners. Among the participants, 38,5% self-reported as hypertensive, but 41,7% from total had altered BP. Among healthy runners, it was found alterations on BP (42%), AC (9.8%) and 6% presented moderated cardiovascular risk. It was observed that 34% already suffered some injuries and 67.7% derived from training and competition. Age, distance of the running and practice of other activities were associated with presence of injury. The results of the present study allow us to conclude that road runners have a physical condition that facilitates the cardiovascular event during the competition and that contributes to the injuries. Thus it suggests that campaigns need to be done in order to make the runner aware about its health condition.
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- 2013
7. Associação dos fatores de risco para doenças cardiovasculares e qualidade de vida entre servidores da saúde Association of cardiovascular disease risk factors and quality of life among health workers
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Matheus Barel, Juliana Cavalcante de Andrade Louzada, Henrique Luiz Monteiro, and Sandra Lia do Amaral
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Hipertensão ,Diabetes ,Sedentarismo ,Obesidade ,Hypertension ,Sedentarism ,Obesity ,Sports ,GV557-1198.995 - Abstract
Inúmeros problemas de saúde pública são atribuídos aos fatores induzidos pelo ambiente de trabalho que, associados ao estilo de vida sedentário, contribuem para deteriorar a qualidade de vida do trabalhador. Neste sentido, procuramos descrever o perfil, avaliar a condição física e o risco cardiovasculares dos funcionários da saúde. Foram avaliados 198 funcionários (46 ± 10 anos) que responderam uma anamnese e foram submetidos a avaliações bioquímicas, antropométricas e funcionais. Os valores foram apresentados como média ± desvio padrão da média. Foram utilizados testes de qui-quadrado (x²), taxa de "odds" e correlações de Pearson (p < 0,05). Dos avaliados, 54% apresentaram peso corporal elevado, 33% pressão arterial (PA) elevada e 56% eram sedentários. Colesterol total (CT), lipoproteína de baixa densidade e triglicerídeos (TG) acima da normalidade foram encontrados em 49%, 41% e 24% dos servidores, respectivamente e, 75% apresentaram taxas reduzidas de lipoproteína de alta densidade. As análises de x² e taxa de "odds" apontaram que os valores de TG e PA foram significativamente maiores para homens comparados às mulheres. Capacidade cardiorrespiratória ruim foi verificada em 56% dos homens e 23% das mulheres, além disso, a flexibilidade apresentou-se com níveis insatisfatórios para homens e mulheres. Observou-se que o índice de massa corporal se correlacionou positivamente com PA, CT e TG. Constatou-se, também que a idade foi fator agravante para PA, flexibilidade, CT e glicemia nas mulheres. Dessa forma, a prevalência de múltiplos fatores de risco nestes trabalhadores demonstra tratar-se de população não assistida pela saúde, motivo pelo qual sugere-se conscientização sobre sua própria saúde evitando a aposentadoria precoceSeveral public health problems are due to environmental work, which, associated with sedentarism, contribute to reduce the quality of life of the worker. Therefore, this study aimed to describe the profile, evaluate the physical condition and cardiovascular risk factor of health workers. Health workers (198) from Bauru and Jau cities (46 ± 10 years old) were assessed. They answered a questionnaire and participated in antrophometric and functional assessments. Values are presented as means ± SD. Qui-squared (x²) analysis, odds rate and Pearson correlation were used (p < 0.05). From all workers evaluated, 54% presented high body weight, 33% high blood pressure and 56% were sedentary. Total cholesterol (TC), low-density lipoprotein and triglycerides (TG) above normal values were found in 49%, 41% and 24% of the individuals, respectively, and 75% presented low values of high-density lipoprotein. Qui-squared analysis and odds rate confirmed that TG and blood pressure (BP) were higher for men compared with women. Physical capacity was bad for 56% of men and 23% of women. Also, flexibility was unsatisfactory for men and women. Body mass index correlated positively with BP, TC and TG. Aging was an important risk factor for BP, TC, blood glucose, body mass index and flexibility only for women (p < 0.05). The prevalence of multiple risk factors among these health workers shows that this population is not adequately assisted. For this reason, a program from the government in order to make this population recognize such problems and avoid premature retirement is suggested
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- 2010
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8. Efects of the physical training, based in ergospyrometric evaluation, in the aerobic capacity of volley players – physical training in volley players
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Newton Nunes, Roberto Kalozdi, Sandra Lia do Amaral, José Elias de Proença, Ana Maria W. Braga, Maria J. N. N. Alves, Carlos Eduardo Negrão, and Cláudia Lúcia de Moraes Forjaz
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treinamento físico ,voleibol ,limiares ventilatórios ,Education - Abstract
The prescription of the exercise training intensity based on ventilatory thresholds, determined by ergoespirometric evaluations, has been very useful for athletes. The aim of the present study was to verify the effect of a short period of exercise training, based on the ventilatory thresholds on physical capacity of volleyball players. Six male volleyball players (25±4 years) were studied during the competitive phase of the State Championship. The players were submitted to two maximal ergoespirometric evaluations on the treadmill with a three-month interval. In this period, the aerobic training was performed with an intensity between the anaerobic threshold and the respiratory compensation point, and the anaerobic training on an intensity above the respiratory compensation point. Exercise training caused a significant increase in maximal oxygen uptake (48.6±3.0 vs. 51.0±3.0 ml.kg-1.min-1, p < 0.05), but without significant alteration in maximal power, maximal heart rate and maximal ventilation. The values of oxygen uptake and heart rate, in which the ventilatory thresholds were achieved, tended to be higher after exercise training. In conclusion, a short period of three months of exercise training based on the ventilatory thresholds is effective in increasing the maximal oxygen uptake in volleyball players and tends to increase the ventilatory thresholds.
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- 2008
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9. Comparação entre a prescrição de intensidade de treinamento físico baseada na avaliação ergométrica convencional e na ergoespirométrica
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Maria Urbana Pinto Brandão Rondon, Cláudia Lúcia de Moraes Forjaz, Newton Nunes, Sandra Lia do Amaral, Antonio Carlos Pereira Barretto, and Carlos Eduardo Negrão
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prescrição de exercício ,freqüência cardíaca ,consumo de oxigênio máximo ,Diseases of the circulatory (Cardiovascular) system ,RC666-701 - Abstract
OBJETIVO: Comparar os limites inferiores (L.inf.) e superiores (L.sup.) da prescrição de treinamento físico aeróbio determinada pelo teste ergométrico convencional (60-70% do VO2máx estimado ou 70-85% da FCmáx atingida), com a prescrição obtida pelo teste ergoespirométrico [limiar anaeróbio (LA) e ponto de compensação respiratória (PCR)]. MÉTODOS: Realizaram teste ergoespirométrico progressivo até a exaustão 47 homens (30±5 anos), divididos em subgrupos, de acordo com a velocidade da esteira durante o teste (4 ou 5mph) e a capacidade física medida [baixa (BCF) e moderada (MCF)]. RESULTADOS: Os L.inf. de prescrição indireta apresentaram valores de VO2 e FC significantemente maiores que os valores de VO2 e FC no LA (4mph= 34,4±4,5 vs 19,6±4,6 e 5mph= 28,9±2 vs 18,9±5,4, e BCF= 32,0±4,1 vs 17,2±2,8 e MCF= 31,6±4,9 vs 21,1±5,7(mlO2.kg-1.min-1) e (4mph = 128,9±7,8 vs 113,1± 15,6 e 5mph= 130,3±5,2 vs 114,1± 18,9, e BCF= 127,6±7,2 vs 109,3±13,2 e MCF= 131,2± 5,7 vs 117,4± 19,2bpm). Os L.sup. de prescrição indireta no grupo de 4mph e BCF apresentaram valores de VO2 significantemente maiores que os valores medidos no PCR (40,1±5,3 vs 32,2±4,3 e 37,4±4,8 vs 30,6±2,5 mlO2.kg-1.min-1, respectivamente), e valores de FC semelhantes aos medidos no PCR. CONCLUSÃO: Os L.inf. da prescrição indireta de treinamento físico superestimam o LA, enquanto os L.sup. parecem adequados somente para indivíduos ativos com MCF.
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- 1998
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10. A saúde do trabalhador no projeto qualidade de vida: dois anos aprendendo com a intervenção
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Rafael Martins Andrade, Luana Urbano Pagan, Natália Paulo Vieira, Henrique Luiz Monteiro, and Sandra Lia do Amaral
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Science ,Social Sciences - Abstract
Introdução: Nos últimos anos, devido à industrialização e ao sedentarismo, os fatores de risco das doenças arteriais coronarianas vêm aumentando. Dentre eles podemos citar obesidade, hipertensão arterial, diabetes e dislipidemia. A Hipertensão Arterial é uma doença multifatorial e assintomática, sendo caracterizada pela manutenção de níveis elevados de pressão arterial, o que contribui para aumentar a morbidade e mortalidade cardiovascular. Funcionários da área da saúde, submetidos a ritmos intensos de trabalho, geralmente apresentam altas taxas de fatores de risco para doenças cardiovasculares. O exercício, por sua vez, tem sido considerado uma das principais formas de tratamento não-farmacológico dessa patologia. Com esse intuito, o “Projeto Qualidade de Vida (PQV)” surgiu como uma parceria entre a Divisão Regional de Saúde de Bauru (DIR-X) e o Depto. de Educação Física da Unesp/Bauru. O projeto foi implantado para avaliar os fatores de risco desta população e proporcionar uma mudança de estilo de vida por meio de exercícios regulares. Objetivos: Investigar os efeitos benéficos do exercício físico orientado no controle dos níveis pressóricos dos participantes do PQV. Métodos: Indivíduos de ambos os sexos (30 a 70 anos), foram submetidos a diversos procedimentos que incluíam: anamnese, avaliação da condição sócio-econômica, escolaridade e risco coronariano, avaliações antropométricas, hemodinâmicas e funcionais (teste ergométrico), que foram realizados no Laboratório de Avaliação e Prescrição de Exercício (LAPE). Após os testes foram realizadas prescrições individualizadas, de acordo com a condição física. As aulas ocorreram nas dependências do Departamento de Educação Física, Unesp/Bauru, com 3 sessões semanais de 90 minutos cada. Resultados: Dentre os resultados mais significativos do PQV, destaca-se o de uma aluna de 49 anos, hipertensa há 8 anos, que faz uso de uma combinação de 3 fármacos antihipertensivos. Após 2 meses de freqüência ao programa de exercícios, a aluna apresentou uma queda de 30 mmHg na pressão arterial sistólica de repouso (de 140 para 110 mmHg, 21%) e de 14 mmHg na pressão arterial diastólica (de 96 para 82 mmHg, 15%). Mediante estas reduções pressóricas houve indicativo de redução de dosagem de seu medicamento em 50% pelo cardiologista. Estes resultados demonstram a grande eficiência do exercício físico como coadjuvante ao tratamento farmacológico da hipertensão. Importante ressaltar que a integração médico e educador físico pode resultar em importante parceria para o controle e tratamento da hipertensão arterial.
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- 2010
11. Dexamethasone Does Not Inhibit Treadmill Training–Induced Angiogenesis in Myocardium: Role of MicroRNA-126 Pathway
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Francine Duchatsch, Naiara A. Herrera, Carlos Ferreira dos Santos, Thiago José Dionísio, Sandra Lia do Amaral, Lidieli P. Tardelli, Universidade Estadual Paulista (Unesp), and Universidade de São Paulo (USP)
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Male ,0301 basic medicine ,medicine.medical_specialty ,Nitric Oxide Synthase Type III ,Angiogenesis ,Microvascular Rarefaction ,Neovascularization, Physiologic ,030204 cardiovascular system & hematology ,Dexamethasone ,Running ,Microcirculation ,03 medical and health sciences ,0302 clinical medicine ,Physical Conditioning, Animal ,Internal medicine ,polycyclic compounds ,Animals ,Medicine ,Phosphorylation ,Rats, Wistar ,Glucocorticoids ,Microvessel ,Protein kinase B ,Pharmacology ,business.industry ,Myocardium ,Skeletal muscle ,Adaptation, Physiological ,Capillaries ,MicroRNAs ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Blood pressure ,PRESSÃO SANGUÍNEA ,Cardiology and Cardiovascular Medicine ,business ,Microvascular Density ,Proto-Oncogene Proteins c-akt ,hormones, hormone substitutes, and hormone antagonists ,Signal Transduction ,medicine.drug - Abstract
Made available in DSpace on 2021-06-25T11:02:57Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-12-01 Dexamethasone (DEX) has important anti-inflammatory activities; however, it induces hypertension and skeletal muscle microcirculation rarefaction. Nevertheless, nothing is known about DEX outcomes on cardiac microcirculation. By contrast, exercise training prevents skeletal and cardiac microvessel loss because of microRNA expression and a better balance between their related angiogenic and apoptotic proteins in spontaneously hypertensive rats. The purpose of this study was to investigate whether DEX and/or exercise training could induce microRNA alterations leading to cardiac angiogenesis or microvascular rarefaction. Animals performed 8 weeks of exercise training and were treated with DEX (50 μg/kg per day, subcutaneously) for 14 days. Cardiovascular parameters were measured, and the left ventricle muscle was collected for analyses. DEX treatment increased arterial pressure and did not cause cardiac microcirculation rarefaction. Treadmill training prevented the DEX-induced increase in arterial pressure. In addition, training, regardless of DEX treatment, increased microRNA-126 expression, phospho-protein kinase B/protein kinase B, and endothelial nitric oxide synthase levels associated with cardiac angiogenesis. In conclusion, this study suggests, for the first time, that treadmill training induces myocardial angiogenesis because of angiogenic pathway improvement associated with an increase in microRNA-126. Furthermore, DEX, per se, did not cause capillary density alterations and did not attenuate cardiac angiogenesis induced by training. Joint Graduate Program in Physiological Sciences PIPGCF UFSCar/UNESP Department of Biological Sciences Bauru School of Dentistry University of São Paulo Department of Physical Education School of Sciences São Paulo State University (UNESP) Joint Graduate Program in Physiological Sciences PIPGCF UFSCar/UNESP Department of Physical Education School of Sciences São Paulo State University (UNESP)
- Published
- 2020
12. Papel das citocinas inflamatórias no remodelamento cardíaco de ratos infartados e tratados com dexametasona
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Cardoso, Sandra Lia Do Amaral
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o objetivo deste estudo será avaliar os efeitos do tratamento com dexametasona nas respostas hemodinâmica, neural e inflamatória de ratos infartados e identificar as citocinas que possam ser utilizadas como alvos terapêuticos no remodelamento cardíaco em um modelo experimental de infarto crônico. É hipótese deste trabalho que o tratamento farmacológico com dexametasona na fase crônica do infarto possa identificar citocinas inflamatórias que tenham papel importante na modulação da estrutura cardíaca, diminuição da deposição de colágeno e angiogênese, resultando em uma melhora da função cardíaca. Uma vez identificados estes alvos terapêuticos, espera-se que a inibição de pelo menos uma citocina inflamatória tenha um papel cardioprotetor em ratos infartados.
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- 2022
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13. EFEITOS VASCULARES DO TREINAMENTO FÍSICO
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Alison Pires de Oliveira Lara, Vinícius Ferreira de Paula, Lidieli P. Tardelli, Sandra Lia do Amaral, and Diego D Santos
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General Earth and Planetary Sciences ,General Environmental Science - Published
- 2019
14. Brazilian Guidelines of Hypertension - 2020
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Weimar Kunz Sebba, Barroso, Cibele Isaac Saad, Rodrigues, Luiz Aparecido, Bortolotto, Marco Antônio, Mota-Gomes, Andréa Araujo, Brandão, Audes Diógenes de Magalhães, Feitosa, Carlos Alberto, Machado, Carlos Eduardo, Poli-de-Figueiredo, Celso, Amodeo, Décio, Mion Júnior, Eduardo Costa Duarte, Barbosa, Fernando, Nobre, Isabel Cristina Britto, Guimarães, José Fernando, Vilela-Martin, Juan Carlos, Yugar-Toledo, Maria Eliane Campos, Magalhães, Mário Fritsch Toros, Neves, Paulo César Brandão Veiga, Jardim, Roberto Dischinger, Miranda, Rui Manuel Dos Santos, Póvoa, Sandra C, Fuchs, Alexandre, Alessi, Alexandre Jorge Gomes de, Lucena, Alvaro, Avezum, Ana Luiza Lima, Sousa, Andrea, Pio-Abreu, Andrei Carvalho, Sposito, Angela Maria Geraldo, Pierin, Annelise Machado Gomes de, Paiva, Antonio Carlos de Souza, Spinelli, Armando da Rocha, Nogueira, Nelson, Dinamarco, Bruna, Eibel, Cláudia Lúcia de Moraes, Forjaz, Claudia Regina de Oliveira, Zanini, Cristiane Bueno de, Souza, Dilma do Socorro Moraes de, Souza, Eduardo Augusto Fernandes, Nilson, Elisa Franco de Assis, Costa, Elizabete Viana de, Freitas, Elizabeth da Rosa, Duarte, Elizabeth Silaid, Muxfeldt, Emilton, Lima Júnior, Erika Maria Gonçalves, Campana, Evandro José, Cesarino, Fabiana, Marques, Fábio, Argenta, Fernanda Marciano, Consolim-Colombo, Fernanda Spadotto, Baptista, Fernando Antonio de, Almeida, Flávio Antonio de Oliveira, Borelli, Flávio Danni, Fuchs, Frida Liane, Plavnik, Gil Fernando, Salles, Gilson Soares, Feitosa, Giovanio Vieira da, Silva, Grazia Maria, Guerra, Heitor, Moreno Júnior, Helius Carlos, Finimundi, Isabela de Carlos, Back, João Bosco de, Oliveira Filho, João Roberto, Gemelli, José Geraldo, Mill, José Marcio, Ribeiro, Leda A Daud, Lotaif, Lilian Soares da, Costa, Lucélia Batista Neves Cunha, Magalhães, Luciano Ferreira, Drager, Luis Cuadrado, Martin, Luiz César Nazário, Scala, Madson Q, Almeida, Marcia Maria Godoy, Gowdak, Marcia Regina Simas Torres, Klein, Marcus Vinícius Bolívar, Malachias, Maria Cristina Caetano, Kuschnir, Maria Eliete, Pinheiro, Mario Henrique Elesbão de, Borba, Osni, Moreira Filho, Oswaldo, Passarelli Júnior, Otavio Rizzi, Coelho, Priscila Valverde de Oliveira, Vitorino, Renault Mattos, Ribeiro Junior, Roberto, Esporcatte, Roberto, Franco, Rodrigo, Pedrosa, Rogerio Andrade, Mulinari, Rogério Baumgratz de, Paula, Rogério Toshiro Passos, Okawa, Ronaldo Fernandes, Rosa, Sandra Lia do, Amaral, Sebastião R, Ferreira-Filho, Sergio Emanuel, Kaiser, Thiago de Souza Veiga, Jardim, Vanildo, Guimarães, Vera H, Koch, Wille, Oigman, and Wilson, Nadruz
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Hypertension ,Humans ,Brazil - Published
- 2021
15. Perindopril Reduces Arterial Pressure and Does Not Inhibit Exercise-Induced Angiogenesis in Spontaneously Hypertensive Rats
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Francine Duchatsch, Carlos Alberto Vicentini, Anderson G. Macedo, Sandra Lia do Amaral, Thalles Fernando Rocha Ruiz, Danyelle S. Miotto, Universidade Estadual Paulista (Unesp), and Universidade Estadual Paulista (UNESP)
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Male ,0301 basic medicine ,medicine.medical_specialty ,ACE inhibitors ,hypertension ,Physical Exertion ,Neovascularization, Physiologic ,Lumen (anatomy) ,Angiotensin-Converting Enzyme Inhibitors ,030204 cardiovascular system & hematology ,physical training ,03 medical and health sciences ,0302 clinical medicine ,Rats, Inbred SHR ,Internal medicine ,Heart rate ,Perindopril ,Animals ,Medicine ,Myocyte ,Arterial Pressure ,Rats, Wistar ,Antihypertensive Agents ,Pharmacology ,Soleus muscle ,business.industry ,Skeletal muscle ,Disease Models, Animal ,030104 developmental biology ,Endocrinology ,Blood pressure ,medicine.anatomical_structure ,Hypertension ,Cardiology and Cardiovascular Medicine ,business ,Perfusion ,medicine.drug ,circulatory and respiratory physiology - Abstract
Made available in DSpace on 2022-04-28T17:21:56Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-04-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Sympathetic activity, arteriolar structure, and angiogenesis are important mechanisms modulating hypertension and this study aimed to analyze the effects of perindopril treatment, associated or not with exercise training, on the mechanisms that control blood pressure (BP) in hypertensive rats. Spontaneously hypertensive rats (SHR) were allocated into 4 groups: 1/sedentary (S); 2/perindopril (P, 3.0 mg/kg/d); 3/trained (T); and 4/trained + perindopril (TP). Wistar rats were used as normotensive sedentary control group. SHR were assigned to undergo a treadmill training (T) or were kept sedentary. Heart rate, BP, sympathetic activity to the vessels (LF-SBP), and skeletal muscle and myocardial morphometric analyses were performed. BP was significantly lower after all 3 strategies, compared with S and was accompanied by lower LF-SBP (-76%, -53%, and -44%, for P, T, and TP, respectively). Arteriolar vessel wall cross-sectional area was lower after treatments (-56%, -52%, and -56%, for P, T, and TP, respectively), and only TP presented higher arteriolar lumen area. Capillary rarefaction was present in soleus muscle and myocardium in S group and both trained groups presented higher vessel density, although perindopril attenuated this increase in soleus muscle. Although myocyte diameter was not different between groups, myocardial collagen deposition area, higher in S group, was lower after 3 strategies. In conclusion, we may suggest that perindopril could be an option for the hypertensive people who practice exercise and need a specific pharmacological treatment to reach a better BP control, mainly because training-induced angiogenesis is an important response to facilitate blood flow perfusion and oxygen uptake and perindopril did not attenuate this response. PIPGCF UFSCar UNESP, Joint Grad Program Physiol Sci, Sao Carlos, SP, Brazil Inst Biosci Humanities & Exact Sci UNESP, Sch Sci, Dept Biol, Sao Jose Do Rio Preto, SP, Brazil UNESP, Sch Sci, Dept Biol Sci, Bauru, SP, Brazil UNESP, Sch Sci, Dept Phys Educ, Bauru, SP, Brazil PIPGCF UFSCar UNESP, Joint Grad Program Physiol Sci, Sao Carlos, SP, Brazil Inst Biosci Humanities & Exact Sci UNESP, Sch Sci, Dept Biol, Sao Jose Do Rio Preto, SP, Brazil UNESP, Sch Sci, Dept Biol Sci, Bauru, SP, Brazil UNESP, Sch Sci, Dept Phys Educ, Bauru, SP, Brazil FAPESP: 2017/00509-1 CAPES: 001 CAPES: 1692103 CAPES: 88882.426908/2019-01 FAPESP: 2017/14405-3
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- 2021
16. Differential effects of dexamethasone on arterial stiffness, myocardial remodeling and blood pressure between normotensive and spontaneously hypertensive rats
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Naiara A. Herrera, Francine Duchatsch, Sandra Lia do Amaral, Lidieli P. Tardelli, Katashi Okoshi, Carlos Alberto Vicentini, and Universidade Estadual Paulista (Unesp)
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collagen ,medicine.medical_specialty ,hypertension ,Diastole ,Hemodynamics ,Blood Pressure ,010501 environmental sciences ,Toxicology ,01 natural sciences ,Dexamethasone ,vessel remodeling ,03 medical and health sciences ,Vascular Stiffness ,Internal medicine ,medicine.artery ,Rats, Inbred SHR ,medicine ,Thoracic aorta ,Animals ,Rats, Wistar ,Pulse wave velocity ,030304 developmental biology ,0105 earth and related environmental sciences ,0303 health sciences ,glucocorticoids ,business.industry ,cardiac hypertrophy ,heart rate variability ,medicine.disease ,Rats ,Blood pressure ,medicine.anatomical_structure ,Heart Injuries ,Ventricle ,Hypertension ,cardiovascular system ,Cardiology ,Arterial stiffness ,business ,medicine.drug - Abstract
Made available in DSpace on 2021-06-25T10:53:51Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-01-01 Dexamethasone (DEX)-induced hypertension is observed in normotensive rats, but little is known about the effects of DEX on spontaneously hypertensive animals (SHR). This study aimed to evaluate the effects of DEX on hemodynamics, cardiac hypertrophy and arterial stiffness in normotensive and hypertensive rats. Wistar rats and SHR were treated with DEX (50 μg/kg s.c., 14 d) or saline. Pulse wave velocity (PWV), echocardiographic parameters, blood pressure (BP), autonomic modulation and histological analyses of heart and thoracic aorta were performed. SHR had higher BP compared with Wistar, associated with autonomic unbalance to the heart. Echocardiographic changes in SHR (vs. Wistar) were suggestive of cardiac remodeling: higher relative wall thickness (RWT, +28%) and left ventricle mass index (LVMI, +26%) and lower left ventricle systolic diameter (LVSD, −19%) and LV diastolic diameter (LVDD, −10%), with slightly systolic dysfunction and preserved diastolic dysfunction. Also, SHR had lower myocardial capillary density and similar collagen deposition area. PWV was higher in SHR due to higher aortic collagen deposition. DEX-treated Wistar rats presented higher BP (~23%) and autonomic unbalance. DEX did not change cardiac structure in Wistar, but PWV (+21%) and aortic collagen deposition area (+21%) were higher compared with control. On the other side, DEX did not change BP or autonomic balance to the heart in SHR, but reduced RWT and LV collagen deposition area (−12% vs. SHRCT). In conclusion, the results suggest a differential effect of dexamethasone on arterial stiffness, myocardial remodeling and blood pressure between normotensive and spontaneously hypertensive rats. Joint Graduate Program in Physiological Sciences PIPGCF UFSCar/UNESP Department of Biology School of Sciences São Paulo State University (UNESP) Department of Medical Clinic Botucatu Medical School São Paulo State University (UNESP) Department of Physical Education School of Sciences São Paulo State University (UNESP) Joint Graduate Program in Physiological Sciences PIPGCF UFSCar/UNESP Department of Biology School of Sciences São Paulo State University (UNESP) Department of Medical Clinic Botucatu Medical School São Paulo State University (UNESP) Department of Physical Education School of Sciences São Paulo State University (UNESP)
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- 2021
17. Diretrizes Brasileiras de Hipertensão Arterial – 2020
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Juan Carlos Yugar-Toledo, Elizabeth S. Muxfeldt, Rodrigo P. Pedrosa, JM Ribeiro, Fábio Argenta, Bruna Eibel, Ronaldo Fernandes Rosa, Gil F. Salles, Madson Q. Almeida, Carlos Alberto Machado, Frida Liane Plavnik, José Fernando Vilela-Martin, Leda A Daud Lotaif, Marco A. Mota-Gomes, Fernanda Spadotto Baptista, Alexandre Jorge Gomes de Lucena, Roberto Dischinger Miranda, Claudia Regina de Oliveira Zanini, Rogério Toshiro Passos Okawa, Luciano F. Drager, Audes D. M. Feitosa, Marcia Maria Godoy Gowdak, Emilton Lima Júnior, Ana Luiza Lima Sousa, Carlos Eduardo Poli-de-Figueiredo, Andréa Araujo Brandão, Sandra Lia do Amaral, Maria Eliete Pinheiro, Helius Carlos Finimundi, Eduardo Augusto Fernandes Nilson, Luiz César Nazário Scala, Fabiana Marques, Heitor Moreno Junior, Flávio A. O. Borelli, Luiz Aparecido Bortolotto, Elizabete Viana de Freitas, Marcus Vinícius Bolívar Malachias, José Geraldo Mill, Otávio Rizzi Coelho, Roberto Jorge da Silva Franco, S Kaiser, LS Costa, Armando da Rocha Nogueira, Wille Oigman, Luis Cuadrado Martin, Osni Moreira Filho, Paulo César Brandão Veiga Jardim, Wilson Nadruz, Márcia Regina Simas Torres Klein, Cláudia Lúcia de Moraes Forjaz, Elisa Franco de Assis Costa, Rogério Baumgratz de Paula, Maria Cristina Caetano Kuschnir, Cristiane Bueno de Souza, D Souza, Roberto Esporcatte, Giovanio Vieira da Silva, Mario Fritsch Neves, Alvaro Avezum, Priscila Valverde de Oliveira Vitorino, Vanildo Guimarães, Gilson Soares Feitosa, João Roberto Gemelli, Fernando Almeida, Thiago Veiga Jardim, Rogério Andrade Mulinari, Oswaldo Passarelli Junior, Cibele Isaac Saad Rodrigues, Annelise Machado Gomes de Paiva, Angela Maria Geraldo Pierin, Maria Eliane Campos Magalhães, Erika Maria Gonçalves Campana, Renault Mattos Ribeiro Junior, Isabel Cristina Britto Guimarães, Vera H. Koch, Lucélia Batista Neves Cunha Magalhães, Andrei C. Sposito, João Bosco de Oliveira Filho, Eduardo Costa Duarte Barbosa, Isabela de Carlos Back, Décio Mion Júnior, Sandra C. Fuchs, Celso Amodeo, Fernando Nobre, Antonio Carlos de Souza Spinelli, Fernanda Marciano Consolim-Colombo, Nelson Dinamarco, Rui Manuel dos Santos Póvoa, Mario Henrique Elesbão de Borba, Grazia Maria Guerra, Weimar Kunz Sebba Barroso, Evandro José Cesarino, Sebastião R. Ferreira-Filho, Andrea Pio-Abreu, Elizabeth da Rosa Duarte, Flávio Danni Fuchs, Alexandre Alessi, Universidade Federal de Goiás (UFG), Liga de Hipertensão Arterial, Faculdade de Ciências Médicas e da Saúde, Instituto do Coração (InCor), Centro Universitário CESMAC, Universidade do Estado do Rio de Janeiro (UERJ), Universidade Federal de Pernambuco (UFPE), Pronto Socorro Cardiológico de Pernambuco (PROCAPE), Pontifícia Universidade Católica do Rio Grande do Sul, Universidade Federal de São Paulo (UNIFESP), Universidade de São Paulo (USP), Serviço Hipertensão e Cardiometabolismo da Santa Casa de Porto Alegre, Hospital São Francisco, Universidade Federal da Bahia (UFBA), Faculdade Estadual de Medicina de São José do Rio Preto, Faculdade de Medicina da Universidade Federal do Rio Grande do Sul (UFRGS), Universidade Federal do Paraná (UFPR), Hospital Agamenom Magalhães, Hospital Alemão Oswaldo Cruz, Universidade Estadual de Campinas (UNICAMP), Cardiocentro, Universidade Federal do Rio de Janeiro (UFRJ), BA - Brasil, Fundação Universitária de Cardiologia (IC/FUC), Centro Universitário da Serra Gaúcha (FSG), Universidade Federal do Pará (UFPA), Ministério da Saúde, SC - Brasil, Universidade Estácio de Sá (UNESA), Universidade Iguaçu (UNIG), Pesquisa e Assistência ao Hipertenso (AREPAH), MT - Brasil, Programa de Pós-Graduação em Medicina da Universidade Nove de Julho (UNINOVE), Instituto Dante Pazzanese de Cardiologia, Hospital de Clínicas de Porto Alegre, Universidade Santo Amaro (UNISA), Universidade Federal de Santa Catarina (UFSC), Hospital Israelita Albert Einstein, Universidade Federal do Espírito Santo (UFES), MG - Brasil, Hospital do Coração (HCor), Instituto Estadual de Cardiologia Aloysio de Castro, Universidade Estadual Paulista (Unesp), Sociedade Brazileira de Nefrologia, Pontifícia Universidade Católica do Paraná, Pontifícia Universidade Católica de Goiás, Cardios Vita Centro de Medicina Cardiológica, Hospital Pró-Cradíaco, Avancor Cardiologia, Universidade Estadual de Maringá (UEM), Faculdade de Ciências Médicas da Santa Casa de São Paulo, Universidade Federal de Uberlândia (UFU), and Hospital Getúlio Vargas
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SciELO ,medicine.medical_specialty ,business.industry ,MEDLINE ,030204 cardiovascular system & hematology ,03 medical and health sciences ,0302 clinical medicine ,RC666-701 ,Primary prevention ,Epidemiology ,medicine ,Diseases of the circulatory (Cardiovascular) system ,Hypertension diagnosis ,Cardiology and Cardiovascular Medicine ,Intensive care medicine ,business ,PREVENÇÃO PRIMÁRIA - Abstract
Made available in DSpace on 2021-06-25T11:03:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2021-03-01. Added 1 bitstream(s) on 2021-07-15T14:36:02Z : No. of bitstreams: 1 S0066-782X2021000400516.pdf: 3972880 bytes, checksum: b9bdbad26f21c4bcb57e0ea9683f5e70 (MD5) Universidade Federal de Goiás Liga de Hipertensão Arterial Pontifícia Universidade Católica de São Paulo Faculdade de Ciências Médicas e da Saúde Instituto do Coração (InCor) Centro Universitário CESMAC, AL - Brasil Universidade Federal de Pernambuco Pronto Socorro Cardiológico de Pernambuco (PROCAPE) Pontifícia Universidade Católica do Rio Grande do Sul Universidade Federal de São Paulo (UNIFESP) Hospital das Clínicas da Faculdade de Medicina da USP Serviço Hipertensão e Cardiometabolismo da Santa Casa de Porto Alegre Hospital das Clínicas da Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo Hospital São Francisco Universidade Federal da Bahia (UFBA) BA - Brasil Faculdade Estadual de Medicina de São José do Rio Preto Faculdade de Medicina da Universidade Federal do Rio Grande do Sul (UFRGS) Universidade Federal do Paraná (UFPR) Hospital Agamenom Magalhães Hospital Alemão Oswaldo Cruz Universidade de São Paulo (USP) Universidade Estadual de Campinas (UNICAMP) Cardiocentro Universidade Federal do Rio de Janeiro (UFRJ) Universidade Estadual de Santa Cruz BA - Brasil Instituto de Cardiologia Fundação Universitária de Cardiologia (IC/FUC) Centro Universitário da Serra Gaúcha (FSG) Universidade Federal do Pará (UFPA) Ministério da Saúde Departamento de Cardiogeriatria da Sociedade Brazileira de Cardiologia Hospital Nossa Senhora da Conceição (HNSC) SC - Brasil Universidade Estácio de Sá (UNESA) Hospital de Clínicas da Universidade Federal do Paraná (HC/UFPR) Universidade Iguaçu (UNIG) Faculdade de Ciências Farmacêuticas de Ribeirão Preto da Universidade de São Paulo Associação Ribeirãopretana de Ensino Pesquisa e Assistência ao Hipertenso (AREPAH) Faculdade de Medicina de Ribeirão Preto da Universidade de São Paulo MT - Brasil Programa de Pós-Graduação em Medicina da Universidade Nove de Julho (UNINOVE) Instituto Dante Pazzanese de Cardiologia Hospital de Clínicas de Porto Alegre Escola Bahiana de Medicina e Saúde Pública BA - Brasil Universidade Santo Amaro (UNISA) SC - Brasil, Universidade Federal de Santa Catarina (UFSC) Hospital Israelita Albert Einstein Centro de Ciências da Saúde Universidade Federal do Espírito Santo Faculdade Ciências Médicas de Minas Gerais MG - Brasil Hospital Felício Rocho MG - Brasil Hospital do Coração (HCor) Instituto Estadual de Cardiologia Aloysio de Castro Centro Universitário de Tecnologia e Ciência (UniFTC) BA - Brasil Universidade Estadual Paulista (UNESP) Faculdade de Medicina da Universidade Federal de Mato Grosso MT - Brasil Sociedade Brazileira de Nefrologia Pontifícia Universidade Católica do Paraná Pontifícia Universidade Católica de Goiás Cardios Vita Centro de Medicina Cardiológica Hospital Pró-Cradíaco MG - Brasil, Universidade Federal de Juiz de Fora Avancor Cardiologia Universidade Estadual de Maringá Faculdade de Ciências Médicas da Santa Casa de São Paulo Universidade Federal de Uberlândia MG - Brasil Hospital Getúlio Vargas Universidade Estadual Paulista (UNESP)
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- 2021
18. Dexamethasone and Training-Induced Cardiac Remodeling Improve Cardiac Function and Arterial Pressure in Spontaneously Hypertensive Rats
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Francine Duchatsch, Lidieli P. Tardelli, Katashi Okoshi, Naiara A. Herrera, Carlos Alberto Vicentini, Carlos Ferreira dos Santos, Thalles Fernando Rocha Ruiz, Sandra Lia do Amaral, Universidade Estadual Paulista (Unesp), and Universidade de São Paulo (USP)
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Cardiac function curve ,medicine.medical_specialty ,hypertension ,Anti-Inflammatory Agents ,Physical exercise ,030204 cardiovascular system & hematology ,Dexamethasone ,RATOS ,03 medical and health sciences ,0302 clinical medicine ,physical exercise ,Internal medicine ,Physical Conditioning, Animal ,parasitic diseases ,Medicine ,echocardiography ,Animals ,Pharmacology (medical) ,Arterial Pressure ,Rats, Wistar ,030304 developmental biology ,Pharmacology ,0303 health sciences ,Ventricular Remodeling ,business.industry ,Synthetic glucocorticoids ,synthetic glucocorticoids ,Rats ,Blood pressure ,Treatment Outcome ,Hypertension ,Cardiology ,cardiac function ,Cardiology and Cardiovascular Medicine ,business ,medicine.drug - Abstract
Made available in DSpace on 2020-12-10T20:10:32Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-08-28 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Introduction: Dexamethasone (DEX)-induced hypertension and cardiac remodeling are still unclear, especially in spontaneously hypertensive rats (SHR). On the other side, exercise training is a good strategy to control hypertension. Therefore, this study investigated the effects of DEX treatment and physical training on arterial pressure and cardiac remodeling in SHR. Material and Methods: SHR underwent treadmill training (5 days/week, 1h/session, at 50-60% of maximal capacity, 0% degree, 75 days) and received low-dose of DEX (50 mu g/kg,s.c.) during the last 15 days. Sedentary Wistar rats (W) were used as control. Echocardiography and artery catheterization were performed for cardiac remodeling and function, arterial pressure and autonomic nervous system analyses. In addition, left ventricle (LV) capillary density, myocyte diameter and collagen deposition area were analyzed using specific histological staining. Results: Low-dose of DEX treatment did not exacerbate arterial pressure of SHR and trained groups had lower values, regardless of DEX. DEX and training decreased relative left ventricle wall thickness (RWT) and determined LV angiogenesis (+19%) and lower collagen deposition area (-22%). In addition, it determined increased left ventricular diastolic diameter. These changes were followed by improvements on systolic and diastolic function, since it was observed increased posterior wall shortening velocity (PWSV) and reduced isovolumetric relaxation time (IVRT). Conclusion: In conclusion, this study is unique to indicate that low-dose of DEX treatment does not exacerbate arterial pressure in SHR and, when associated with training, it improves LV systolic and diastolic function, which may be due to LV angiogenesis and reduction of wall collagen deposition area. UNESP, PIPGCF UFSCar, Joint Grad Program Physiol Sci, Sao Carlos, SP, Brazil Sao Paulo State Univ Unesp, Sch Sci, Dept Biol Sci, Bauru, SP, Brazil Sao Paulo State Univ Unesp, Botucatu Med Sch, Dept Internal Med, Botucatu, SP, Brazil Univ Sao Paulo, Bauru Sch Dent, Dept Biol Sci, Bauru, SP, Brazil Sao Paulo State Univ Unesp, Sch Sci, Dept Phys Educ, Bauru, SP, Brazil UNESP, PIPGCF UFSCar, Joint Grad Program Physiol Sci, Sao Carlos, SP, Brazil Sao Paulo State Univ Unesp, Sch Sci, Dept Biol Sci, Bauru, SP, Brazil Sao Paulo State Univ Unesp, Botucatu Med Sch, Dept Internal Med, Botucatu, SP, Brazil Sao Paulo State Univ Unesp, Sch Sci, Dept Phys Educ, Bauru, SP, Brazil FAPESP: 2017/00509-1 FAPESP: 2015/03965-2 FAPESP: 2018/00567-4 FAPESP: 2017/14405-3 CAPES: 88882.426908/2019-01 FAPESP: 2016-12532-5 CAPES: 88882.426901/2019-01
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- 2020
19. In vivo vascular rarefaction and hypertension induced by dexamethasone are related to phosphatase PTP1B activation not endothelial metabolic changes
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Jeannette Vasquez-Vivar, Naiara A. Herrera, Allison Kahlke, Sandra Lia do Amaral, Francine Duchatsch, Universidade Estadual Paulista (Unesp), and Medical College of Wisconsin
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0301 basic medicine ,endocrine system ,medicine.medical_specialty ,Microvascular Rarefaction ,Bradykinin ,Glucocorticoid receptor ,Biochemistry ,Dexamethasone ,Article ,Nitric oxide ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Insulin resistance ,In vivo ,Physiology (medical) ,Internal medicine ,polycyclic compounds ,medicine ,Animals ,Endothelial dysfunction ,Endothelial Cells ,medicine.disease ,VEGF ,Phosphoric Monoester Hydrolases ,Rats ,030104 developmental biology ,Endocrinology ,chemistry ,Hypertension ,030217 neurology & neurosurgery ,Glucocorticoid ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug - Abstract
Made available in DSpace on 2020-12-12T01:12:35Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-05-20 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) National Institutes of Health Glucocorticoids have important anti-inflammatory and immunomodulatory activities. Dexamethasone (Dex), a synthetic glucocorticoid, induces insulin resistance, hyperglycemia, and hypertension. The hypertensive mechanisms of Dex are not well understood. Previously, we showed that exercise training prior to Dex treatment significantly decreases blood vessel loss and hypertension in rats. In this study, we examined whether the salutary effects of exercise are associated with an enhanced metabolic profile. Analysis of the NAD and ATP content in the tibialis anterior muscle of trained and non-trained animals indicated that exercise increases both NAD and ATP; however, Dex treatment had no effect on any of the experimental groups. Likewise, Dex did not change NAD and ATP in cultured endothelial cells following 24 h and 48 h of incubation with high concentrations. Reduced VEGF-stimulated NO production, however, was verified in endothelial cultured cells. Reduced NO was not associated with changes in survival or the BH4 to BH2 ratio. Moreover, Dex had no effect on bradykinin- or shear-stress-stimulated NO production, indicating that VEGF-stimulated eNOS phosphorylation is a target of Dex's effects. The PTP1B inhibitor increased NO in Dex-treated cells in a dose-dependent fashion, an effect that was replicated by the glucocorticoid receptor inhibitor, RU486. In combination, these results indicate that Dex-induced endothelial dysfunction is mediated by glucocorticoid receptor and PTP1B activation. Moreover, since exercise reduces the expression of PTP1B and normalized insulin resistance in aging rats, our findings indicate that exercise training by reducing PTP1B activity counteracts Dex-induced hypertension in vivo. Joint Graduate Program in Physiological Sciences PIPGCF UFSCar/UNESP, Rodovia Washington Luiz, São Carlos/SP Department of Physical Education São Paulo State University School of Sciences, Av. Eng. Luiz Edmundo Carrijo Coube Department of Biophysics Redox Biology Program Medical College of Wisconsin Joint Graduate Program in Physiological Sciences PIPGCF UFSCar/UNESP, Rodovia Washington Luiz, São Carlos/SP Department of Physical Education São Paulo State University School of Sciences, Av. Eng. Luiz Edmundo Carrijo Coube FAPESP: #2017/00509-1 FAPESP: 2016/12532-5 FAPESP: 2017/14405-3 FAPESP: 2018/06998-7 National Institutes of Health: R01 NS081936
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- 2020
20. Standardization of a new non-invasive device for assessment of arterial stiffness in rats: Correlation with age-related arteries’ structure
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Maria Tereza Jordão, Silvia Lacchini, Danyelle S. Miotto, Thales F.R. Ruiz, Sandra Lia do Amaral, Carlos Alberto Vicentini, Mayara F. Fabricio, Carlos Ferreira dos Santos, Lisete Compagno Michelini, Universidade Estadual Paulista (Unesp), and Universidade de São Paulo (USP)
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Femoral ,Aging ,Standardization ,Computer science ,Clinical Biochemistry ,010501 environmental sciences ,Collagen deposition ,01 natural sciences ,Correlation ,03 medical and health sciences ,COLÁGENO ,medicine ,lcsh:Science ,Pulse wave velocity ,Aorta ,pOpetⓇ 1.0 system for small animals ,030304 developmental biology ,0105 earth and related environmental sciences ,Carotid ,0303 health sciences ,Reproducibility ,Non invasive ,Stiffness ,Gold standard (test) ,Medicine and Dentistry ,medicine.disease ,Medical Laboratory Technology ,Hypertension ,Arterial stiffness ,lcsh:Q ,medicine.symptom ,Biomedical engineering - Abstract
Pulse wave velocity (PWV) has become a gold standard index to quantify the stiffness of the aorta and is a predictor of cardiovascular events. A recent paper compared the pOpmètreⓇ, a device for measuring the finger-toe PWV, with other techniques and demonstrated its accuracy and validity. However, human devices do not allow the advancement of our knowledge on conditioning mechanisms. Based on its human validation, a new device, pOpetⓇ 1.0 system was designed for estimation of PWV in small animals and this present study aimed to standardize the pOpetⓇ 1.0 for estimation of arterial stiffness in rats, and to confirm its liability and stability as well as the reproducibility of assessments. Therefore several precautions were taken into consideration like as the correct position of the animal and photodiodes according to manufacturers’ suggestions. Results indicated that estimation of PWV through the new pOpetⓇ 1.0 device exhibits good internal consistency, stability and objectivity in all tests performed between days and evaluators. Importantly, data suggest for the first time that this new device is able to detect changes in arterial stiffness that are conditioned by age and pressure-related arterial remodeling. • This new pOpetⓇ device is able to detect changes in vessel structure. • This new pOpetⓇ device exhibits good internal consistency, stability and objectivity in all tests performed • Correct position of the animal and photodiodes are crucial to obtain a very stable signal., Graphical abstract Image, graphical abstract
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- 2020
21. MicroRNA-126 upregulation, induced by training, plays a role in controlling microcirculation in dexamethasone treated rats
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Sandra Lia do Amaral, Thiago José Dionísio, Naiara A. Herrera, Lidieli P. Tardelli, Carlos Ferreira dos Santos, André Luis Shinohara, Francine Duchatsch, Universidade Estadual Paulista (Unesp), and Universidade de São Paulo (USP)
- Subjects
0301 basic medicine ,Male ,medicine.medical_specialty ,Skeletal muscle ,030209 endocrinology & metabolism ,Apoptosis ,Biochemistry ,Dexamethasone ,Microcirculation ,RATOS ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Downregulation and upregulation ,Internal medicine ,Physical Conditioning, Animal ,microRNA ,Adrenal Glands ,Medicine ,Animals ,Rats, Wistar ,Muscle, Skeletal ,Molecular Biology ,Protein kinase B ,Glucocorticoids ,Arterial pressure ,business.industry ,Body Weight ,Hemodynamics ,Organ Size ,Capillaries ,Up-Regulation ,MicroRNAs ,030104 developmental biology ,Blood pressure ,medicine.anatomical_structure ,Physical training ,Microvascular Rarefaction ,business ,Proto-Oncogene Proteins c-akt ,medicine.drug - Abstract
Made available in DSpace on 2020-12-12T02:24:06Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-04-05 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Microcirculation maintenance is associated with microRNAs. Nevertheless, the role of microRNAs induced by training in preventing dexamethasone (DEX)-induced microvascular rarefaction remains unknown. The study aim was to investigate if training-induced microRNAs are able to improve microcirculation proteins and prevent DEX-induced microvascular rarefaction. Rats underwent training for 8 weeks and then were treated with DEX (50 μg/kg per day, s.c.) for 14 days. Arterial pressure was measured and tibialis anterior (TA) muscle was collected for analyses. DEX induced hypertension concomitantly with capillary density loss (CD, −23.9%) and decrease of VEGF (−43.0%), p-AKT/AKT (−39.6%) and Bcl-2 (−23.0%) and an increase in caspase-3-cleaved protein level (+34.0%) in TA muscle. Training upregulated microRNA-126 expression (+13.1%), prevented VEGF (+61.4%), p-AKT/AKT (+37.7%), Bcl-2 (+7.7%) decrease and caspase-3-cleaved (−23.1%) increase associated with CD (+54.7%) reduction and hypertension prevention. MiRNA-126 upregulation, induced by training, plays a role in controlling microcirculation, which may be a potential target against DEX-induced microvascular rarefaction. Joint Graduate Program in Physiological Sciences PIPGCF UFSCar/UNESP, Rodovia Washington Luiz, km 235 Monjolinho 676 Department of Biological Sciences Bauru School of Dentistry University of São Paulo, Alameda Octávio Pinheiro Brisolla 9-75 Department of Physical Education - São Paulo State University (UNESP) School of Sciences, Av. Eng. Luiz Edmundo Carrijo Coube, 14-01, Vargem Limpa Joint Graduate Program in Physiological Sciences PIPGCF UFSCar/UNESP, Rodovia Washington Luiz, km 235 Monjolinho 676 Department of Physical Education - São Paulo State University (UNESP) School of Sciences, Av. Eng. Luiz Edmundo Carrijo Coube, 14-01, Vargem Limpa FAPESP: 2015/03965-2 FAPESP: 2016/12532-5 FAPESP: 2017/00509-1 FAPESP: 2017/14405-3 FAPESP: 2018/06998-7 CAPES: 88882.426901/2019-01 CAPES: 88882.426908/2019-01
- Published
- 2019
22. Exercise Training Prevents Dexamethasone-induced Rarefaction
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Naiara A. Herrera, Isley Jesus, Carlos Ferreira dos Santos, Evandro Jose Dionisio, Thiago José Dionísio, and Sandra Lia do Amaral
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Male ,0301 basic medicine ,medicine.medical_specialty ,Angiogenesis ,Microvascular Rarefaction ,Anti-Inflammatory Agents ,030204 cardiovascular system & hematology ,Antioxidants ,Dexamethasone ,Superoxide dismutase ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Physical Conditioning, Animal ,Internal medicine ,medicine ,Animals ,Rats, Wistar ,Muscle, Skeletal ,Pharmacology ,biology ,Skeletal muscle ,Rats ,Vascular endothelial growth factor ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,chemistry ,Catalase ,Apoptosis ,biology.protein ,Cardiology and Cardiovascular Medicine ,medicine.drug - Abstract
Dexamethasone (DEX) causes rarefaction. In contrast, training (T) prevents rarefaction and stimulates angiogenesis. This study investigated the mechanisms responsible for the preventive role of T in DEX-induced rarefaction. Rats underwent T or were kept sedentary (8 weeks) and were treated with DEX or saline during the following 14 days. Tibialis anterior muscle was used for measurements of capillary density (CD), capillary-to-fiber ratio (C:F ratio), superoxide dismutase CuZn (SOD-1), superoxide dismutase MnSOD (SOD-2), catalase (CAT) mRNA as well as SOD-1, SOD-2, CAT, vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor-2 (VEGF-R2), cyclooxygenase-2 (COX-2), B-cell lymphoma 2 (Bcl-2), Bd-2-like protein 4 (Bax), p-Bax, and caspase-3 cleaved protein levels. DEX decreased CD (-38.1%), C:F ratio (-30.0%), VEGF (-19.0%), VEGFR-2 (-20.1%), COX-2 (-22.8%), Bcl-2 (-20.5%), Bcl-2/Bax ratio (-13.7%), p-Bax/Bax (-20.0%) and increased SOD-2 (+41.6%) and caspase-3 cleaved (+24.1%). Conversely, T prevented reductions in CD (+54.2%), C:F ratio (+32.9%), VEGF (+25.3%), VEGFR-2 (+22.2%), COX-2 (+31.5%), Bcl-2 (+35.5%), Bcl-2/Bax ratio (+19.9%), p-Bax/Bax (+32.1%), and caspase-3 cleaved increase (-7.8%). T increased CAT mRNA (+21.5%) in the DEX-treated group. In conclusion, T prevented the DEX-induced rarefaction by increasing antioxidant enzymes resulting in a better balance between apoptotic and anti-apoptotic protein levels.
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- 2017
23. The Impact of Aging and Estimated Training Status on Blood Pressure and Antihypertensive Medicine Consumption
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Sandra Lia do Amaral, Bruna Camilo Turi, Henrique Luiz Monteiro, André Mourão Jacomini, Anderson Bernardino da Silva, Roberta Fernanda da Silva, and Anderson Saranz Zago
- Subjects
medicine.medical_specialty ,Functional training ,Blood pressure ,business.industry ,Physical therapy ,medicine ,Physical exercise ,business ,Older people - Abstract
The purpose of this study was to analyze the effect of aging and the estimated training status (TS) on the blood pressure (BP) and antihypertensive medicine consumption (AMC) which are supposed to be high in older people compared with adults. Participants (n=396) performed the following tests: Functional fitness battery test proposed by AAHPERD, blood pressure measurement and a questionnaire about habits of physical exercise and medicine consumptions. In summary, older group present high level of blood pressure and medicine consumptions compared with adults groups (BP - 125±5/77±9 vs 119±12/78±9 mmHg and AMC - 1.8±0.1 vs 1.4±0.1 respectively). However, when participants were divided according to TS, good level of TS was associated with low level of blood pressure. These results were not observed in adults group. Thus, the current study contributes to establish the good level of TS as a marker of good level of blood pressure, especially in older group.
- Published
- 2017
24. Training counteracts DEX-induced microvascular rarefaction by improving the balance between apoptotic and angiogenic proteins
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Naiara A. Herrera, Jesus Carlos Andreo, Isley Jesus, Sandra Lia do Amaral, Carlos Ferreira dos Santos, Universidade Estadual Paulista (Unesp), and Universidade de São Paulo (USP)
- Subjects
Male ,medicine.medical_specialty ,Steroid hormone ,Angiogenesis ,Clinical Biochemistry ,Microvascular Rarefaction ,MÚSCULO ESQUELÉTICO ,Anti-Inflammatory Agents ,Hemodynamics ,Skeletal muscle ,030209 endocrinology & metabolism ,Apoptosis ,Biochemistry ,Dexamethasone ,Exercise training ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Endocrinology ,Enos ,Internal medicine ,Physical Conditioning, Animal ,medicine ,Animals ,Angiogenic Proteins ,Rats, Wistar ,Molecular Biology ,Pharmacology ,Soleus muscle ,biology ,Chemistry ,Organic Chemistry ,biology.organism_classification ,Rats ,Vascular endothelial growth factor ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Hypertension ,Blood pressure ,Apoptosis Regulatory Proteins ,medicine.drug - Abstract
Made available in DSpace on 2020-12-12T02:23:59Z (GMT). No. of bitstreams: 0 Previous issue date: 2020-04-01 Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) This work investigated the mechanisms induced by exercise training that may contribute to attenuate dexamethasone (DEX)-induced microvascular rarefaction and hypertension. Wistar rats underwent training protocol or were kept sedentary for 8 weeks. Dexamethasone was administered during the following 14-days and hemodynamic parameters were recorded at the end. Capillary density (CD) and capillary-to-fiber ratio (C:F ratio) were obtained in soleus muscle (SOL). Also, vascular endothelial growth factor (VEGF), vascular endothelial growth factor receptor-2 (VEGFR-2), endothelial nitric oxide synthase (eNOS), B-cell lymphoma 2 (Bcl-2), Bcl-2-like protein 4 (Bax), p-BAX and caspase-3 cleaved protein levels were analyzed. DEX treatment significantly increased blood pressure (+14%), which was associated with reduced C:F ratio (−41.0%) and CD (−43.1%). Reduction of vessel density was associated with decreased VEGF (−15.6%), VEGFR-2 (−14.6%), Bcl-2 (−18.4%), Bcl-2/Bax ratio (−29.0%) and p-Bax/Bax (−25.4%), and also with increased caspase-3 cleaved protein level (25%). Training, on the other hand, prevented microvessels loss by mitigating all proteins changes induced by DEX. In addition, angiogenic and apoptotic proteins were significantly correlated with CD, which, in turn, was associated with blood pressure. Therefore, we may point out that exercise training is a good strategy to attenuate DEX-induced microvascular rarefaction in soleus muscle and this response involves a better balance between apoptotic and angiogenic proteins, which may contribute for the attenuation of hypertension. Department of Physical Education – São Paulo State University (UNESP) Science Faculty, Av. Eng. Luiz Edmundo Carrijo Coube, 14-01 – Vargem Limpa Joint Graduate Program in Physiological Sciences PIPGCF UFSCar/UNESP, Rodovia Washington Luiz, km 235 Monjolinho, 676 Department of Biological Sciences Bauru School of Dentistry University of São Paulo, Alameda Octávio Pinheiro Brisolla, 9-75 Department of Physical Education – São Paulo State University (UNESP) Science Faculty, Av. Eng. Luiz Edmundo Carrijo Coube, 14-01 – Vargem Limpa Joint Graduate Program in Physiological Sciences PIPGCF UFSCar/UNESP, Rodovia Washington Luiz, km 235 Monjolinho, 676
- Published
- 2019
25. Exercise Training‐MicroRNA‐126 Upregulation is Associated with Prevention of Dexamethasone‐Mediated Microvascular Rarefaction
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Thiago José Dionísio, Naiara A. Herrera, Lidieli P. Tardelli, Carlos Ferreira dos Santos, Sandra Lia do Amaral, Jeannette Vasquez-Vivar, and Francine Duchatsch Ribeiro Souza
- Subjects
medicine.medical_specialty ,business.industry ,Biochemistry ,Endocrinology ,Downregulation and upregulation ,Internal medicine ,microRNA ,Genetics ,Medicine ,Microvascular Rarefaction ,business ,Molecular Biology ,Dexamethasone ,Biotechnology ,medicine.drug - Published
- 2019
26. Dexamethasone‐Induced Effects on Autonomic Balance, Arterial Stiffness and Cardiac Remodeling in Sedentary and Trained Spontaneously Hypertensive Rats
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Francine Duchatsch, Naiara A. Herrera, Sandra Lia do Amaral, Lidieli P. Tardelli, Carlos Ferreira dos Santos, Carlos Alberto Vicentini, Katashi Okoshi, Thalles Fernando Rocha Ruiz, and Thiago José Dionísio
- Subjects
medicine.medical_specialty ,business.industry ,medicine.disease ,Biochemistry ,Internal medicine ,Genetics ,medicine ,Arterial stiffness ,Cardiology ,business ,Molecular Biology ,Dexamethasone ,Biotechnology ,Balance (ability) ,medicine.drug - Published
- 2019
27. Impacto do Tabagismo Passivo na Resposta Pressórica à Epinefrina e Felipressina em Ratos Hipertensos 1K1C Tratados ou não com Atenolol
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Gabriela M. Oliveira, Carlos Ferreira dos Santos, Thiago José Dionísio, Adriana Maria Calvo, Camila A. Fleury, Flávio A. Faria, Elizandra P. M. Almeida, and Sandra Lia do Amaral
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03 medical and health sciences ,0302 clinical medicine ,Traditional medicine ,business.industry ,Medicine ,030204 cardiovascular system & hematology ,Cardiology and Cardiovascular Medicine ,business - Published
- 2019
28. Anomalous Origin of the Vertebral Artery
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Mateus José da Silva, Sandra Lia do Amaral, Rogério Leone Buchaim, Gustavo Lopes Toledo, Juliane Ruiz de Souza, André Luis Shinohara, Jesus Carlos Andreo, Beatriz Sobrinho Sangalette, Laís Rinaldi, Universidade de Sâo Paulo, Universidade de Marilia, Universidade Estadual Paulista (UNESP), and Universidade de Marília
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Left vertebral artery ,Aortic arch ,Foramen magnum ,Histology ,business.industry ,Vertebral artery ,Cell Biology ,Anatomy ,030204 cardiovascular system & hematology ,Surgical procedures ,clinical anatomy ,03 medical and health sciences ,0302 clinical medicine ,medicine.anatomical_structure ,medicine.artery ,Anatomic variation ,medicine ,Foramen ,vertebral artery ,Common carotid artery ,business ,030217 neurology & neurosurgery ,Subclavian artery - Abstract
Made available in DSpace on 2022-04-29T08:45:26Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-12-01 Introduction The present article presents a rare case of variation of the left vertebral artery, which originated from the aortic arch, between the common carotid artery and the subclavian artery, although classic descriptions of the vertebral artery indicate its origin at the subclavian artery, where it penetrates the foramen transverse and ascends cervically to the foramen magnum. Case presentation The anatomical peculiarity of the vertebral artery is evidenced. The origin of the vertebral artery was identified directly from the aortic arch in a dissected human specimen. Conclusion In view of the numerous surgical procedures performed in this area, the clear understanding of its topographic layout is indispensable, and might change surgical procedures performed in the region. Department of Biological Sciences-Anatomy Faculdade de Odontologia de Bauru Universidade de Sâo Paulo Faculdade de Odontologia de Marilia Universidade de Marilia, Avenida Waldemar Kireff 185, apt, 22, 17525-020 Department of Physical Education Faculdade de Ciências Universidade Estadual Paulista Department of Oral Maxillofacial Surgery Faculdade de Odontologia de Marília Universidade de Marília Department of Physical Education Faculdade de Ciências Universidade Estadual Paulista
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- 2018
29. High-intensity resistance training attenuates dexamethasone-induced muscle atrophy
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Sandra Lia do Amaral, Andre L. O. Krug, James W. E. Rush, Carlos Ferreira dos Santos, Anderson G. Macedo, and Anderson Saranz Zago
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0301 basic medicine ,medicine.medical_specialty ,Physiology ,medicine.medical_treatment ,030209 endocrinology & metabolism ,Biology ,Body weight ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,0302 clinical medicine ,Physiology (medical) ,Internal medicine ,medicine ,Saline ,Dexamethasone ,PI3K/AKT/mTOR pathway ,High intensity ,Resistance training ,Skeletal muscle ,Muscle atrophy ,Surgery ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,Neurology (clinical) ,medicine.symptom ,medicine.drug - Abstract
Introduction In this study we investigated the effects of high-intensity resistance training (RT) on dexamethasone (DEX)-induced muscle atrophy in flexor hallucis longus (FHL), tibialis anterior (TA), and soleus (SOL) muscles. Methods Rats underwent either high-intensity RT or were kept sedentary. In the last 10 days they received either DEX (0.5 mg/kg/day, intraperitoneally) or saline. Results DEX reduced body weight (-21%), food intake (-28%), FHL and TA muscle mass (-20% and -18%, respectively), and increased muscle-specific ring finger 1 (MuRF-1) protein level (+37% and +45.5%). RT attenuated FHL muscle atrophy through a combination of low increase in MuRF-1 protein level (-3.5%) and significant increases in mammalian target of rapamycin (mTOR) (+63%) and p70S6K (+46% and +49% for control and DEX, respectively) protein levels. Conclusion RT attenuated DEX-induced muscle atrophy through a combination of increases in mTOR and p70S6K protein levels and a low increase in MuRF-1 protein level.
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- 2016
30. Short-term exposure to dexamethasone promotes autonomic imbalance to the heart before hypertension
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Paula B. Constantino, Sandra Lia do Amaral, Francine Duchatsch, Thiago José Dionísio, Lidieli P. Tardelli, Carlos Ferreira dos Santos, Naiara A. Herrera, Aline Mio Martuscelli, Mayara F. Fabricio, Universidade Estadual Paulista (Unesp), and Universidade de São Paulo (USP)
- Subjects
0301 basic medicine ,medicine.medical_specialty ,medicine.medical_treatment ,030204 cardiovascular system & hematology ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Gene expression ,Heart rate ,polycyclic compounds ,Internal Medicine ,medicine ,Autonomic nervous system ,Saline ,Dexamethasone ,glucocorticoids ,business.industry ,Cardiac muscle ,blood pressure ,030104 developmental biology ,Endocrinology ,medicine.anatomical_structure ,Blood pressure ,HORMÔNIOS GLICOCORTICOIDES ,Autonomic imbalance ,Cardiology and Cardiovascular Medicine ,business ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug - Abstract
Made available in DSpace on 2018-12-11T17:24:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-08-01 Hypertension is one of the chronic side effects of dexamethasone (DEX) treatment; however, almost nothing is known about its acute effects. Therefore, the aim of this study was to investigate the possible mechanisms involved in blood pressure control after acute or short-term DEX treatment in adult animals. Eighty Wistar rats were divided into four groups: C1 and C5, for rats treated with saline for 1 or 5 days, respectively; D1 and D5, for rats treated with DEX for 1 or 5 days, respectively (decadron, 1 mg/kg, i.p.). Heart rate was increased in DEX treatment, but arterial pressure and cardiac muscle mass were not altered. Only few and isolated changes on gene expression and protein level of renin-angiotensin system components were observed. Five days of DEX treatment, but not one day, determined an increase in sympathetic component of spectral analysis (+75.93%, P
- Published
- 2018
31. Preliminary study about the relationship between estimated training status and RAS polymorphisms on blood pressure and ACE activity in the elderly
- Author
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AndréMourão Jacomini, Jose E. Tanus-Santos, Anderson Saranz Zago, Carlos Ferreira dos Santos, Roberta Fernanda da Silva, Thiago José Dionísio, Riccardo Lacchini, Sandra Lia do Amaral, Lucas C. Pinheiro, Universidade Estadual Paulista (Unesp), and Universidade de São Paulo (USP)
- Subjects
Male ,medicine.medical_specialty ,Medicine (General) ,Genotype ,Physical fitness ,Physical exercise ,030204 cardiovascular system & hematology ,Peptidyl-Dipeptidase A ,elderly ,Renin-Angiotensin System ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,R5-920 ,Internal medicine ,Renin–angiotensin system ,Internal Medicine ,medicine ,Humans ,Ace activity ,Alleles ,Aged ,Polymorphism, Genetic ,business.industry ,Hemodynamics ,blood pressure ,030229 sport sciences ,Blood pressure ,Hypertension ,Cardiology ,physical fitness ,Female ,Original Article ,business ,Polymorphisms ,TREINAMENTO FÍSICO ,RAS - Abstract
Made available in DSpace on 2018-12-11T17:24:19Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-06-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Objective: Polymorphisms of the renin angiotensin system (RAS) are associated with increases in blood pressure (BP). Physical exercise has been considered the main strategy to prevent this increase. This study aimed to investigate the relationship between estimated training status (TS), BP and angiotensin-converting enzyme (ACE) activity in elderly people classified as low or high risk to develop hypertension according to genetic profile. Methods: A total of 155 elderly participants performed the following assessments: general functional fitness index (GFFI), systolic BP (SBP) and diastolic BP (DBP), blood collection for ACE activity and analyses of the RAS polymorphisms. Results: Uncontrolled hypertensive (UHT) participants presented higher values of SBP and DBP compared with normotensive (NT) and controlled hypertensive (CHT) participants. No differences were found in ACE activity and GFFI between groups. In the high risk group, UHT presented higher values of SBP and DBP compared with other groups. CHT presented higher values of SBP compared with NT. Furthermore, UHT presented higher values of ACE activity compared with CHT and lower values of GFFI compared with NT. Conclusion: MDA, TIA and TIC genetic combinations were associated with high risk of developing hypertension while the maintenance of good levels of TS was associated with lower BP values and ACE activity. Department of Physical Education São Paulo State University (UNESP) School of Science School of Nursing of Ribeirão Preto USP - University of São Paulo Faculty of Medicine of Ribeirão Preto USP - University of São Paulo Bauru School of D entistry University of São Paulo Department of Physical Education São Paulo State University (UNESP) School of Science FAPESP: Grants no 2015/24847-8 to ASZ.
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- 2018
32. Training Attenuated Hypertension in Dexamethasone‐Treated Wistar Rats and SHR Through Different Mechanisms
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Francice Duchatsch, Sandra Lia do Amaral, Lidieli P. Tardelli, and Naiara A. Herrera
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medicine.medical_specialty ,Endocrinology ,business.industry ,Internal medicine ,Genetics ,medicine ,business ,Molecular Biology ,Biochemistry ,Dexamethasone ,Biotechnology ,medicine.drug - Published
- 2018
33. Non‐invasive assessment of arterial stiffness correlates with age‐related vessel structure: a new Popmètre Device for Rats
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Carlos Alberto Vicentini, Thalles Fernando Rocha Ruiz, Mateus Rossetto Vidal, Lisete Compagno Michelini, Anderson Saranz Zago, Mayara F. Fabricio, Sandra Lia do Amaral, and Maria Tereza Jordão
- Subjects
medicine.medical_specialty ,business.industry ,Non invasive ,medicine.disease ,Biochemistry ,Vessel structure ,Internal medicine ,Age related ,Genetics ,Cardiology ,medicine ,Arterial stiffness ,business ,Molecular Biology ,Biotechnology - Published
- 2018
34. Effect of chronic exercise on fluoride metabolism in fluorosis-susceptible mice exposed to high fluoride
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Liane B. Azevedo, Ruth A. Valentine, Mayara F. Fabricio, Mileni da Silva Fernandes, Sandra Lia do Amaral, Fatemeh Zohoori, Anne Maguire, Marília Afonso Rabelo Buzalaf, Universidade Estadual Paulista (Unesp), Teesside Univ, Universidade de São Paulo (USP), and Newcastle Univ
- Subjects
Male ,medicine.medical_specialty ,Fluorosis, Dental ,Group ii ,Male mice ,lcsh:Medicine ,010501 environmental sciences ,Body weight ,01 natural sciences ,Article ,Bone and Bones ,Fluorides ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Physical Conditioning, Animal ,Internal medicine ,Animals ,Medicine ,lcsh:Science ,0105 earth and related environmental sciences ,Multidisciplinary ,business.industry ,Body Weight ,lcsh:R ,PERDA DE PESO ,030206 dentistry ,Metabolism ,Physical Functional Performance ,High fluoride ,Exercise capacity ,Endocrinology ,chemistry ,Physical performance ,lcsh:Q ,business ,Fluoride - Abstract
Made available in DSpace on 2018-11-26T17:48:14Z (GMT). No. of bitstreams: 0 Previous issue date: 2018-02-16 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Teesside University Newcastle University The present study investigated the effect of chronic exercise on fluoride (F) metabolism in fluorosis-susceptible mice exposed to high-F and explored the relationship between F concentrations in bone and plasma. Thirty male mice were randomised into three groups: Group I (No-F, No-Exercise), Group II (50 ppmF, No-Exercise), Group III (50 ppmF, Exercise). Body weight and physical performance of all mice were measured at baseline and end of experiment. F concentrations of plasma and bone were measured at the end of experiment. Mean plasma F concentration was significantly higher (p < 0.001) in Groups II and III compared with Group I. Mean bone F concentration was also significantly higher (p < 0.01) in Groups II and III compared with Group I. There was a significant correlation (p = 0.01, r = 0.54) between F concentration of plasma and bone. Mean body weight of Group I mice was significantly higher than Group II (p < 0.001) and Group III (p = 0.001) mice at the end of the experiment. This study, which provides the first data on the effect of chronic exercise on F metabolism in fluorosis-susceptible mice, suggests no effect of chronic exercise on F in plasma and bone. However, exposure to high-F resulted in lower body weight and exercise capacity in mice. Sao Paulo State Univ, Sci Fac, Dept Phys Educ, BR-17033360 Bauru, SP, Brazil Teesside Univ, Sch Hlth & Social Care, Middlesbrough TS1 3BA, Cleveland, England Univ Sao Paulo, Bauru Sch Dent, BR-17012901 Bauru, SP, Brazil Newcastle Univ, Sch Dent Sci, Ctr Oral Hlth Res, Newcastle Upon Tyne NE2 4BW, Tyne & Wear, England Sao Paulo State Univ, Sci Fac, Dept Phys Educ, BR-17033360 Bauru, SP, Brazil
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- 2018
35. Aptidão física relacionada à saúde de alunos de graduação em Educação Física
- Author
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Rodrigo Dutra Bergoc, Bruna Camilo Turi-Lynch, Rosana Lisboa Maia, Henrique Luiz Monteiro, Camila Angélica Asahi Mesquita, and Sandra Lia do Amaral
- Subjects
Gynecology ,medicine.medical_specialty ,business.industry ,Physical fitness ,Public Health, Environmental and Occupational Health ,Hemodynamics ,Anthropometry ,Aptidão física, Perfil de Saúde, Estudantes de Ciências da Saúde ,Education ,Blood pressure ,Heart rate ,medicine ,business ,lcsh:L ,Body mass index ,lcsh:Education - Abstract
O objetivo desse estudo foi descrever e analisar o condicionamento físico de alunos de graduação do curso de Educação Física. A amostra foi composta por 110 mulheres e 115 homens. Foram avaliados peso, altura, índice de massa corpórea, porcentagem de gordura (GC), frequência cardíaca, pressão arterial sistólica (PAS) e diastólica, flexibilidade, força e resistência muscular localizada (RML). Como principais resultados observamos maior porcentagem de mulheres com gordura corporal e RML acima dos valores ideais para a idade quando comparados aos homens (GC: 37% vs 4%, p= 0,001; RML: 32% vs 13%, p= 0,001), enquanto houveram mais homens com alteração na PAS quando confrontado com mulheres (22% vs 6%, p= 0,001). Em conclusão, estudantes do sexo masculino apresentaram maiores níveis de aptidão física em relação às mulheres, e parcela expressiva de alunos de Educação Física apresentam alterações nos valores de referência à normalidade para variáveis antropométricas, hemodinâmicas e de desempenho motor.
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- 2017
36. The influence of genetic polymorphisms on performance and cardiac and hemodynamic parameters among Brazilian soccer players
- Author
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Gustavo Pompermaier Garlet, Thiago José Dionísio, Roberto Braga Silva, Evandro José Dionísio, Carlos Ferreira dos Santos, Guilherme Augusto Talamoni, Carlos Rogério Thiengo, Daniel Thomas Brozoski, Sandra Lia do Amaral, Universidade Estadual Paulista (Unesp), Universidade de São Paulo (USP), and Universidade Estadual de Campinas (UNICAMP)
- Subjects
Male ,Genotyping Techniques ,Physiology ,Endocrinology, Diabetes and Metabolism ,Angiotensinogen ,Hemodynamics ,030204 cardiovascular system & hematology ,medicine.disease_cause ,AMP Deaminase ,0302 clinical medicine ,Jumping ,ACE and AGT polymorphisms ,Medicine ,Actinin ,Nutrition and Dietetics ,Ejection fraction ,biology ,Soccer athletes’ performance ,General Medicine ,Sprint ,Cardiology ,Cardiac and hemodynamic parameters ,Brazil ,Adult ,medicine.medical_specialty ,Adolescent ,Athletic Performance ,Peptidyl-Dipeptidase A ,AMPD1 ,ACTN3 ,03 medical and health sciences ,Young Adult ,Physiology (medical) ,Internal medicine ,Heart rate ,Soccer ,Humans ,Genotyping ,Life Style ,Polymorphism, Genetic ,business.industry ,Athletes ,030229 sport sciences ,Sequence Analysis, DNA ,biology.organism_classification ,Blood pressure ,Physical therapy ,business - Abstract
Made available in DSpace on 2018-12-11T16:47:37Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-01-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) This study investigated whether ACTN3 R577X, AMPD1 C34T, I/D ACE, and M235T AGT polymorphisms can affect performance tests such as jumping, sprinting, and endurance in 220 young male athletes from professional minor league soccer team from São Paulo Futebol Clube, Brazil. I/D ACE and M235T AGT polymorphisms were also analyzed according to cardiac and hemodynamic parameters. Athletes were grouped or not by age. DNA from saliva and Taqman assays were used for genotyping 220 athletes and the results were associated with performance tests. Ventricle mass, ventricle end-diastolic diameter, enddiastolic volume, and ejection fraction were assessed by echocardiogram. Arterial pressure, heart rate, and oximetry were assessed by a cardioscope. The main results of this study were that athletes who carried RR/RX (ACTN3) and DD (ACE) genotypes presented better performance during jump and sprint tests. On the other hand, athletes with ID/II genotype presented better results during endurance test, while AGT genotypes did not seem to favor the athletes during the evaluated physical tests. CC genotype (AMPD1) only favored the athletes during 10-m sprint test. Although there are environmental interactions influencing performance, the present results suggest that RR/RX ACTN3 and ACE DD genotypes may benefit athletes in activities that require strength and speed, while II ACE genotype may benefit athletes in endurance activities. This information could help coaches to plan the training session to improve the athletes’ performance. Joint Graduate Program in Physiological Sciences PIPGCF UFSCar/UNESP Bauru School of Dentistry University of São Paulo State University of Campinas Institute of Bioscience University of São Paulo State (UNESP) Physiological Sciences PIPGCF UFSCar/UNESP Joint Graduate Program in Physiological Sciences PIPGCF UFSCar/UNESP Institute of Bioscience University of São Paulo State (UNESP) Physiological Sciences PIPGCF UFSCar/UNESP CNPq: 140206/2011-6 FAPESP: 2011/21586-8
- Published
- 2017
37. Exercise attenuates dexamethasone-induced hypertension through an improvement of baroreflex activity independently of the renin-angiotensin system
- Author
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Carlos Ferreira dos Santos, Carlos C. Crestani, Naiara A. Herrera, Thiago José Dionísio, Sandra Lia do Amaral, Paula B. Constantino, Josiane O. Duarte, Francine Duchatsch, Universidade Estadual Paulista (Unesp), and Universidade de São Paulo (USP)
- Subjects
0301 basic medicine ,medicine.medical_specialty ,Clinical Biochemistry ,Blood Pressure ,030204 cardiovascular system & hematology ,Baroreflex ,Biochemistry ,Dexamethasone ,Renin-Angiotensin System ,Exercise training ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Atrophy ,Heart Rate ,Internal medicine ,Physical Conditioning, Animal ,medicine ,Aerobic exercise ,Animals ,Autonomic nervous system ,Arterial Pressure ,Muscle, Skeletal ,Molecular Biology ,Glucocorticoids ,Arterial pressure ,Pharmacology ,business.industry ,Angiotensin II ,Organic Chemistry ,medicine.disease ,Muscle atrophy ,Exercise Therapy ,Rats ,Muscular Atrophy ,030104 developmental biology ,Blood pressure ,Cardiovascular Diseases ,Hypertension ,medicine.symptom ,business ,PRESSÃO SANGUÍNEA ,medicine.drug - Abstract
Made available in DSpace on 2018-12-11T17:23:40Z (GMT). No. of bitstreams: 0 Previous issue date: 2017-12-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Dexamethasone-induced hypertension may be caused by baroreflex alterations or renin-angiotensin system (RAS) exacerbation. Aerobic training has been recommended for hypertension treatment, but the mechanisms responsible for reduction of arterial pressure (AP) in dexamethasone (DEX) treated rats are still inconclusive.This study evaluated whether mechanisms responsible for training-induced attenuation of hypertension involve changes in autonomic nervous system and in RAS components. Rats underwent aerobic training protocol on treadmill or were kept sedentary for 8 weeks. Additionally, animals were treated with DEX during the last 10 days of exercise. Body weight (BW), AP and baroreflex activity were analyzed. Tibialis anterior (TA), soleus (SOL) and left ventricle (LV) were collected for evaluation of RAS components gene expression and protein levels. Dexamethasone decreased BW (20%), caused TA atrophy (16%) and increased systolic AP (SAP, 16%) as well as decreased baroreflex activity. Training attenuated SAP increase and improved baroreflex activity, although it did not prevent DEX-induced BW reduction and muscle atrophy. Neither DEX nor training caused expressive changes in RAS components. In conclusion, exercise training was effective in attenuating hypertension induced by DEX and this response may be mediated by a better autonomic balance through an improvement of baroreflex activity rather than changes in RAS components. Joint Graduate Program in Physiological Sciences PIPGCF UFSCar/UNESP, Rodovia Washington Luiz, km 235, Monjolinho, 676 Department of Physical Education Science Faculty São Paulo State University (UNESP), Av. Eng. Luiz Edmundo Carrijo Coube, 14-01, Vargem Limpa Department of Biological Sciences Bauru School of Dentistry University of São Paulo, Alameda Octávio Pinheiro Brisolla, 9-75 Laboratory of Pharmacology School of Pharmaceutical Sciences São Paulo State University (UNESP), Rodovia Araraquara Jaú, km 01 – s/n, Campos Ville Joint Graduate Program in Physiological Sciences PIPGCF UFSCar/UNESP, Rodovia Washington Luiz, km 235, Monjolinho, 676 Department of Physical Education Science Faculty São Paulo State University (UNESP), Av. Eng. Luiz Edmundo Carrijo Coube, 14-01, Vargem Limpa Laboratory of Pharmacology School of Pharmaceutical Sciences São Paulo State University (UNESP), Rodovia Araraquara Jaú, km 01 – s/n, Campos Ville CNPq: 140206/2011-6 FAPESP: 2011/21522-0 FAPESP: 2012/03816-9 FAPESP: 2012/14376-0 FAPESP: 2012/19722-3 FAPESP: 2014/18177-7 FAPESP: 2014/23050-6
- Published
- 2017
38. Comparison of epinephrine and felypressin pressure effects in 1K1C hypertensive rats treated or not with atenolol
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Vagner C. Andreo, Carlos Ferreira dos Santos, Flávio A. Faria, Pedro C. Lomba, Sandra Lia do Amaral, Thiago José Dionísio, and Camila A. Fleury
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Male ,HIPERTENSÃO ,Epinephrine ,Blood Pressure ,Vasodilation ,chemistry.chemical_compound ,Heart Rate ,Heart rate ,Animals ,Vasoconstrictor Agents ,Medicine ,Rats, Wistar ,Felypressin ,Kidney ,business.industry ,Atenolol ,Rats ,Anesthesiology and Pain Medicine ,Blood pressure ,medicine.anatomical_structure ,chemistry ,Anesthesia ,Hypertension ,Hypotension ,business ,medicine.drug - Abstract
Epinephrine is considered the gold standard vasoconstrictor for hypertensive patients, but few studies report felypressin’s effects. The present study aimed to analyze and compare the effects of these two vasoconstrictors, injected by the intravenous route, on the arterial pressure of normotensive, hypertensive and atenolol-treated hypertensive rats. The hypertension model was one-kidney-one-clip (1K1C): the main left renal artery was partially constricted and the right kidney was surgically removed in 45-day-old male Wistar rats. 1K1C hypertensive rats received atenolol (90 mg/kg/day) by gavage for 2 weeks. 28–35 days after hypertension induction, a catheter was inserted into the left carotid artery to record direct blood pressure values. The following parameters were recorded: minimal hypotensive response, maximal hypertensive response, response duration and heart rate. Epinephrine, but not felypressin, exerted an important hypotensive action; non-treated hypertensive rats showed more pronounced vasodilation. Treated and non-treated rats showed hypertensive responses of the same magnitudes in all groups; 1K1C atenolol rats showed reduced hypertensive responses to both vasoconstrictors. Felypressin’s response duration was longer than that of epinephrine in all groups. Epinephrine increased heart rate while felypressin reduced this parameter only in the normotensive group. Our results suggest that felypressin has equipotent pressure responses when compared with epinephrine, showing a greater extent of action. Atenolol’s reduction of hypertensive effects surprisingly suggests that atenolol β-blockade may also be important for felypressin’s cardiovascular effect, as is widely known for epinephrine. Our data suggest that felypressin is safe for hypertensive subjects, in particular those receiving atenolol.
- Published
- 2014
39. Influence of training status and eNOS haplotypes on plasma nitrite concentrations in normotensive older adults: a hypothesis-generating study
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Riccardo Lacchini, Sandra Lia do Amaral, Jonas T. C. Sertorio, James W. E. Rush, Átila Alexandre Trapé, Anderson Saranz Zago, Jose E. Tanus-Santos, and Roberta Fernanda da Silva
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Aging ,medicine.medical_specialty ,Nitric Oxide Synthase Type III ,Blood Pressure ,Biology ,chemistry.chemical_compound ,Polymorphism (computer science) ,Enos ,Internal medicine ,ÓXIDO NÍTRICO ,medicine ,Humans ,Allele ,Nitrite ,Exercise ,Genotyping ,Alleles ,Nitrites ,Aged ,Polymorphism, Genetic ,Lipid peroxide ,medicine.diagnostic_test ,Teaching ,Haplotype ,biology.organism_classification ,Surgery ,Endocrinology ,Haplotypes ,chemistry ,Geriatrics and Gerontology ,Lipid profile - Abstract
The purpose of this study was to evaluate the relationship between 3 eNOS gene polymorphisms and training status (TS) in affecting plasma nitrite concentration (NO2) in normotensive adults over 50 years old. Resting blood pressure (BP) was measured in all participants (n = 101). Plasma was taken to analyze: lipid profile, nitrite concentration (NO2) and lipid peroxide levels (T-BARS). Also, genomic DNA was extracted from plasma for genotyping NOS3 polymorphisms (-786T>C; 894G>T; and VNTR in intron 4). TS was determined by one-mile walk test and Functional Fitness Test Battery from AAHPERD (TS1—regular TS; TS2—good TS; and TS3—very good TS). BP was not influenced by TS, but NO2 was 15 % higher in TS3 (123 ± 27 nM) compared to TS-2 (106 ± 22 nM). No differences were found in plasma NO2 in the haplotype analyses. However, the presence of the C allele (T-786C) and ASP allele (Glu298Asp) was found to enhance the correlation between TS and NO2 levels (r = 0.492 in C/4b/ASP haplotype and r = 0.855 in C/4a/ASP haplotype). This study thus identifies NOS3 polymorphism-dependent sensitivity to the effects of physical training on plasma NO2. Maintenance of good levels of training status, in carriers of C allele for T-786C polymorphism, combined with ASP allele for Glu298Asp polymorphism, may result in an increase in the NO2 plasma concentrations, which may reflect improved NO bioavailability in older adult normotensive individuals.
- Published
- 2014
40. Training status as a marker of the relationship between nitric oxide, oxidative stress, and blood pressure in older adult women
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Lucas C. Pinheiro, Anderson Bernardino da Silva, Danielle da Silva Dias, Kátia De Angelis, Átila Alexandre Trapé, André Mourão Jacomini, Hugo Celso Dutra de Souza, Anderson Saranz Zago, Jose E. Tanus-Santos, Janaina de Oliveira Brito, Sandra Lia do Amaral, Roberta Fernanda da Silva, Universidade de São Paulo (USP), Universidade Nove de Julho (UNINOVE), and Universidade Estadual Paulista (Unesp)
- Subjects
0301 basic medicine ,Aging ,medicine.medical_specialty ,Functional training ,Article Subject ,Hemodynamics ,Blood Pressure ,030204 cardiovascular system & hematology ,Nitric Oxide ,Biochemistry ,Nitric oxide ,Body Mass Index ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Absorptiometry, Photon ,Oxygen Consumption ,Internal medicine ,medicine ,Humans ,Treadmill ,lcsh:QH573-671 ,General fitness training ,Aged ,lcsh:Cytology ,Superoxide Dismutase ,Waist-Hip Ratio ,EXERCÍCIO FÍSICO ,VO2 max ,Cell Biology ,General Medicine ,Middle Aged ,Oxidative Stress ,030104 developmental biology ,Endocrinology ,Blood pressure ,chemistry ,Physical therapy ,Exercise Test ,Female ,Body mass index ,Research Article - Abstract
Made available in DSpace on 2018-12-11T17:23:24Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-01-01 The purpose of this study was to evaluate the influence of functional fitness and oxidative capacity on the nitric oxide concentration associated with hemodynamic control in older adult women. The sample consisted of 134 women (65.73 ± 6.14 years old). All subjects underwent a physical examination to assess body mass index, waist-hip ratio, body fat measurement by dual energy X-ray absorptiometry, and blood pressure (BP). Training status (TS) was evaluated by indirect determination of maximal oxygen uptake by a treadmill test using Balke protocol modified for older adults. Functional fitness was also evaluated through a Functional Fitness Battery Test to determine the general fitness functional index (GFFI). All participants were separated according to the functional fitness (TS1, very weak and weak; TS2, regular; TS3, good and very good). Plasma blood samples were used to evaluate prooxidant and antioxidant activity and nitrite and nitrate concentrations. The general results of this study showed that good levels of TS were related to lower levels of lipoperoxidation and protein damage, higher levels of antioxidant, and higher concentration of nitrite and nitrate. This combination may be responsible for the lower levels of BP in subjects with better TS. Ribeirao Preto Medical School, University of Sao Paulo (USP), Avenida Bandeirantes 3900, 14049-900 Ribeirao Preto, SP, Brazil Translational Physiology Laboratory, Universidade Nove de Julho (UNINOVE), Rua Vergueiro 235/249, Liberdade, 01504-001 Sao Paulo, SP, Brazil School of Nursing of Ribeirao Preto, University of Sao Paulo (USP), Avenida Bandeirantes 3900, 14040-902 Ribeirao Preto, SP, Brazil Department of Physical Education, Universidade Estadual Paulista (UNESP), Avenida Engenheiro Luiz Edmundo Carrijo Coube, 14-01, Bairro Vargem Limpa, 17033-360 Bauru, SP, Brazil
- Published
- 2016
41. Exercise training attenuates dexamethasone-induced hypertension by improving autonomic balance to the heart, sympathetic vascular modulation and skeletal muscle microcirculation
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André Luis Shinohara, Naiara A. Herrera, Isley Jesus, Carlos Ferreira dos Santos, Sandra Lia do Amaral, and Thiago José Dionísio
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Male ,Vascular Endothelial Growth Factor A ,medicine.medical_specialty ,Sympathetic nervous system ,Sympathetic Nervous System ,Physiology ,030204 cardiovascular system & hematology ,Dexamethasone ,Microcirculation ,03 medical and health sciences ,0302 clinical medicine ,Heart Rate ,Internal medicine ,Physical Conditioning, Animal ,Heart rate ,Internal Medicine ,medicine ,Aerobic exercise ,Animals ,Arterial Pressure ,Rats, Wistar ,Muscle, Skeletal ,bcl-2-Associated X Protein ,Soleus muscle ,business.industry ,Skeletal muscle ,Heart ,Capillaries ,Rats ,Endocrinology ,Blood pressure ,medicine.anatomical_structure ,Proto-Oncogene Proteins c-bcl-2 ,Hypertension ,Cardiology and Cardiovascular Medicine ,business ,SISTEMA NERVOSO SIMPÁTICO ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Objective Although aerobic exercise training has been recommended as nonpharmacological treatment of high blood pressure, the mechanisms of training-induced blood pressure lowering effects in dexamethasone (DEX)-induced hypertension remain unclear. Therefore, the aim of this study was to investigate the preventive role of exercise training in counteracting DEX-induced hypertension. Methods Rats were submitted to aerobic exercise training for 8 weeks or kept sedentary and then treated with DEX (50 μg/kg/day, s.c.) or saline injections for 14 days. Thereafter, all rats underwent carotid artery catheterization, and cardiovascular autonomic modulation was evaluated by spectral analysis. In addition, soleus muscle was collected for morphometric and protein level analysis. Results DEX treatment increased arterial pressure concomitantly with an increase in low-frequency spectral power of systolic arterial pressure and low frequency in pulse interval (94.11 and 58.58%, respectively), and a decrease in high-frequency spectral power of pulse interval (-12.05%). Capillary density (-25.87%), capillary-to-fibers ratio (-21.22%), vascular endothelial growth factor level (-15.10%), B-cell lymphoma 2 (Bcl-2) level (-16.40%) and Bcl-2/Bcl-2 associated X protein ratio (-27.14%) were all decreased after DEX treatment. Exercise training attenuated DEX-induced increase in arterial pressure accompanied by an attenuation of low-frequency spectral power of systolic arterial pressure, low frequency in pulse interval increases and high-frequency spectral power of pulse interval decrease. Training also prevented the decrease in capillary density (+44.43%), capillary-to-fibers ratio (+36.97%), vascular endothelial growth factor (+16.46%), Bcl-2 (+15.21%) protein level and Bcl-2/Bcl-2-associated X protein ratio (+30.93%). Conclusion These results demonstrate that exercise training improves cardiovascular autonomic balance to the heart associated with an improvement in sympathetic modulation of vascular tone and microcirculatory function in the skeletal muscle of DEX-induced hypertensive rats.
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- 2016
42. Cardiac benefits of exercise training in aging spontaneously hypertensive rats
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Lisete Compagno Michelini, Vicente Lahera, Sandra Lia do Amaral, Luciana Venturini Rossoni, María Miana, Rene R. Caffaro, David Sanz-Rosa, Marcia Kiyomi Koike, Victoria Cachofeiro, and Raphael A. F. Oliveira
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Male ,Aging ,medicine.medical_specialty ,Physiology ,Gene Expression ,Blood Pressure ,Rats, Inbred WKY ,Heart Rate ,Physical Conditioning, Animal ,Rats, Inbred SHR ,Internal medicine ,Internal Medicine ,Animals ,Medicine ,Myocytes, Cardiac ,RNA, Messenger ,Tissue Inhibitor of Metalloproteinase-2 ,Left ventricle hypertrophy ,business.industry ,Myocardium ,Heart ,Coronary Vessels ,Actins ,Rats ,FISIOLOGIA ,Blood pressure ,Hypertension ,Cardiology ,Matrix Metalloproteinase 2 ,Hypertrophy, Left Ventricular ,Collagen ,Cardiology and Cardiovascular Medicine ,business - Abstract
To evaluate the effect of low-intensity chronic exercise training (ExT) on blood pressure (BP), as well as the cardiac alterations associated with hypertension in aging hypertensive rats.Male spontaneously hypertensive rats (SHR; 21 months old) and their normotensive control Wistar-Kyoto (WKY) rats were submitted to low-intensity training protocol for 13 weeks. BP, cardiac morphological and morphometric analysis, as well as gene expression of fibrotic and inflammatory factors were analyzed at the end of the training period.ExT reduced BP and heart rate in aged SHR. Left ventricle hypertrophy, collagen volume fraction and wall-to-lumen ratio of myocardium arterioles were also decreased in trained SHR. However, ExT was unable to reverse the either reduced capillary density or the cardiac myocyte hypertrophy observed in SHR as compared with WKY rats. Trained SHR showed higher metalloproteinase-2/tissue inhibitor metalloproteinase-2 (MMP-2/TIMP-2) ratio and lower levels of α-smooth muscle actin, but similar levels of connective tissue growth factor, transforming growth factor beta or IL-1 beta to that of nontrained SHR.Low to moderate-intensity chronic ExT reverses the cardiac alterations associated with hypertension: myocardial arteriole, left ventricle hypertrophy, collagen content and tachycardia. These changes could be consequence or cause of the reduction in BP observed in trained SHR. In addition, ExT does not worsen the underlying inflammatory burden associated with hypertension. Therefore, the data support a beneficial effect of ExT in aging SHR similar to that reported in young or middle-aged individuals, confirming that exercise is a healthy habit that induces cardiac improvements independently of age.
- Published
- 2011
43. Methodological Considerations to Evaluate the Effect of Physical Activity on Fluoride Metabolism in Children
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Melissa Thiemi Kato, Ruth A. Valentine, Larissa Tercilia Grizzo, Liane B. Azevedo, Sandra Lia do Amaral, Fatemeh Zohoori, Marília Afonso Rabelo Buzalaf, Anne Maguire, and Lilian Aparecida Ferreira
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chemistry.chemical_compound ,chemistry ,business.industry ,Environmental health ,Physical activity ,Medicine ,Physical Therapy, Sports Therapy and Rehabilitation ,Orthopedics and Sports Medicine ,business ,Fluoride - Published
- 2018
44. Low-intensity treadmill exercise-related changes in the rat stellate ganglion neurons
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Helen Tang, Sandra Lia do Amaral, Antonio Augusto Coppi Maciel Ribeiro, Demilto Yamaguchi da Pureza, Cássia T. Bergamaschi, Romeu Rodrigues de Souza, Andrzej Loesch, Renato Albuquerque de Oliveira Cavalcanti, and Mariana Pereira de Melo
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Male ,medicine.medical_specialty ,Mean arterial pressure ,Stellate Ganglion ,Blood Pressure ,Cell Count ,Stereology ,Physical exercise ,Muscle hypertrophy ,RATOS ,Cellular and Molecular Neuroscience ,Heart Rate ,Physical Conditioning, Animal ,Internal medicine ,Heart rate ,Animals ,Medicine ,Rats, Wistar ,Neurons ,Analysis of Variance ,business.industry ,Hypertrophy ,Rats ,medicine.anatomical_structure ,Blood pressure ,Endocrinology ,nervous system ,Stellate ganglion ,Neuron ,business - Abstract
Stellate ganglion (SG) represents the main sympathetic input to the heart. This study aimed at investigating physical exercise-related changes in the quantitative aspects of SG neurons in treadmill-exercised Wistar rats. By applying state-of-the-art design-based stereology, the SG volume, total number of SG neurons, mean perikaryal volume of SG neurons, and the total volume of neurons in the whole SG have been examined. Arterial pressure and heart rate were also measured at the end of the exercise period. The present study showed that a low-intensity exercise training program caused a 12% decrease in the heart rate of trained rats. In contrast, there were no effects on systolic pressure, diastolic pressure, or mean arterial pressure. As to quantitative changes related to physical exercise, the main findings were a 21% increase in the fractional volume occupied by neurons in the SG, and an 83% increase in the mean perikaryal volume of SG neurons in treadmill-trained rats, which shows a remarkable neuron hypertrophy. It seems reasonable to infer that neuron hypertrophy may have been the result of a functional overload imposed on the SG neurons by initial posttraining sympathetic activation. From the novel stereological data we provide, further investigations are needed to shed light on the mechanistic aspect of neuron hypertrophy: what role does neuron hypertrophy play? Could neuron hypertrophy be assigned to the functional overload induced by physical exercise?
- Published
- 2009
45. Is Gender Crucial for Cardiovascular Adjustments Induced by Exercise Training in Female Spontaneously Hypertensive Rats?
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Sandra Lia do Amaral, Lisete Compagno Michelini, L. S. Sanchez, Rosemeire Coimbra, Janaina M. Potenza, Luciana Venturini Rossoni, Universidade de São Paulo (USP), and Universidade Estadual Paulista (Unesp)
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Male ,kidney ,medicine.medical_specialty ,Rest ,Physical Exertion ,arterioles ,Hemodynamics ,Lumen (anatomy) ,Blood Pressure ,Physical exercise ,Hindlimb ,Kidney ,Rats, Inbred WKY ,Ventricular Function, Left ,Coronary Circulation ,Physical Conditioning, Animal ,Rats, Inbred SHR ,Internal medicine ,myocardium ,Internal Medicine ,Animals ,Medicine ,capillaries ,cardiovascular diseases ,skeletal muscle ,Muscle, Skeletal ,Sex Characteristics ,vascular resistance ,business.industry ,Microcirculation ,Skeletal muscle ,Heart ,Organ Size ,Anatomy ,Adaptation, Physiological ,Rats ,Cardiovascular physiology ,medicine.anatomical_structure ,venules ,Ventricle ,Hypertension ,cardiovascular system ,Vascular resistance ,Cardiology ,Female ,business ,circulatory and respiratory physiology - Abstract
Made available in DSpace on 2013-08-12T19:22:58Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-09-01 Made available in DSpace on 2013-09-30T19:21:53Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-09-01 Submitted by Vitor Silverio Rodrigues (vitorsrodrigues@reitoria.unesp.br) on 2014-05-20T15:33:38Z No. of bitstreams: 0 Made available in DSpace on 2014-05-20T15:33:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2008-09-01 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) Evidence of mild hypertension in women and female rats and our preliminary observation showing that training is not effective to reduce pressure in female as it does in male spontaneously hypertensive rats (SHR) prompt us to investigate the effects of gender on hemodynamic pattern and microcirculatory changes induced by exercise training. Female SHR and normotensive controls (Wistar- Kyoto rats) were submitted to training (55% VO2 peak; 3 months) or kept sedentary and instrumented for pressure and hindlimb flow measurements at rest and during exercise. Heart, kidney, and skeletal muscles (locomotor/ nonlocomotor) were processed for morphometric analysis of arterioles, capillaries, and venules. High pressure in female SHR was accompanied by an increased arteriolar wall: lumen ratio in the kidney (+30%; P < 0.01) but an unchanged ratio in the skeletal muscles and myocardium. Female SHR submitted to training did not exhibit further changes on the arteriolar wall: lumen ratio and pressure, showing additionally increased hindlimb resistance at rest (+29%; P < 0.05). on the other hand, female SHR submitted to training exhibited increased capillary and venular densities in locomotor muscles (+50% and 2.3- fold versus sedentary SHR, respectively) and normalized hindlimb flow during exercise hyperemia. Left ventricle pressure and weight were higher in SHR versus WKY rats, but heart performance (positive dP/dt(max) and negative dP/dt(max)) was not changed by hypertension or training, suggesting a compensated heart function in female SHR. In conclusion, the absence of training- induced structural changes on skeletal muscle and myocardium arterioles differed from changes observed previously in male SHR, suggesting a gender effect. This effect might contribute to the lack of pressure fall in trained female SHRs. Univ São Paulo, Inst Biomed Sci, Dept Physiol & Biophys, ICB, BR-05508900 São Paulo, Brazil São Paulo State Univ, UNESP, Fac Sci, Dept Phys Educ, São Paulo, Brazil São Paulo State Univ, UNESP, Fac Sci, Dept Phys Educ, São Paulo, Brazil
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- 2008
46. COX-2 Inhibition Decreases VEGF Expression and Alveolar Bone Loss During the Progression of Experimental Periodontitis in Rats
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Thais Marchini de Oliveira, Sandra Lia do Amaral, Maria Aparecida de Andrade Moreira Machado, Vivien Thiemy Sakai, Tania Mary Cestari, Carlos Ferreira dos Santos, Thiago José Dionísio, and Rumio Taga
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Male ,Vascular Endothelial Growth Factor A ,medicine.medical_specialty ,Pathology ,Blotting, Western ,Alveolar Bone Loss ,Thiazines ,Inflammation ,Meloxicam ,Statistics, Nonparametric ,Random Allocation ,chemistry.chemical_compound ,Internal medicine ,medicine ,Animals ,RNA, Messenger ,Rats, Wistar ,Periodontitis ,Dental alveolus ,Cyclooxygenase 2 Inhibitors ,biology ,Reverse Transcriptase Polymerase Chain Reaction ,business.industry ,medicine.disease ,Rats ,Resorption ,Vascular endothelial growth factor ,Thiazoles ,Endocrinology ,chemistry ,Disease Progression ,biology.protein ,Periodontics ,Immunohistochemistry ,Cyclooxygenase ,medicine.symptom ,business ,medicine.drug - Abstract
Background: Vascular endothelial growth factor (VEGF) is a macromolecule of importance in inflammation that has been implicated in periodontitis. The aims of this study were to investigate VEGF expression during the progression of periodontal disease and to evaluate the effect of a preferential cyclooxygenase (COX)-2 inhibitor meloxicam on VEGF expression and alveolar bone loss in experimentally induced periodontitis. Methods: A total of 120 Wistar rats were randomly separated into groups 1 (control) and 2 (meloxicam, 3mg/kg/day,intraperitoneally,for3,7,14,or30days).Silkligatureswereplacedatthegingivalmarginlevel of the lower right first molar of all rats. VEGF expression was assessed by reverse transcription-polymerase chain reaction (RT-PCR), Western blot (WB), and immunohistochemical (IHC) analyses. The hemiarcades wereprocessedforhistopathologicanalysis.RT-PCRandWBresultsweresubmittedtoanalysisofvariance, the Tukey test, and Pearson correlation analysis (P
- Published
- 2008
47. Congenic strains reveal the effect of the renin gene on skeletal muscle angiogenesis induced by electrical stimulation
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Micheline Resende, Sandra Lia do Amaral, Carol Moreno, and Andrew S. Greene
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Male ,medicine.medical_specialty ,Physiology ,Angiogenesis ,Congenic ,Neovascularization, Physiologic ,Blood Pressure ,Stimulation ,Biology ,Renin-Angiotensin System ,Animals, Congenic ,Lisinopril ,Rats, Inbred BN ,Internal medicine ,Renin ,Renin–angiotensin system ,Genetics ,medicine ,Animals ,Muscle, Skeletal ,Antihypertensive Agents ,Crosses, Genetic ,Chromosome 13 ,Rats, Inbred Dahl ,Body Weight ,Skeletal muscle ,Sodium, Dietary ,Organ Size ,Diet, Sodium-Restricted ,Angiotensin II ,Electric Stimulation ,Rats ,Endocrinology ,medicine.anatomical_structure ,Hypertension ,medicine.drug - Abstract
Previous studies have indicated the importance of angiotensin II (ANG II) in skeletal muscle angiogenesis. The present study explored the effect of regulation of the renin gene on angiogenesis induced by electrical stimulation with the use of physiological, pharmacological, and genetic manipulations of the renin-angiotensin system (RAS). Transfer of the entire chromosome 13, containing the physiologically regulated renin gene, from the normotensive inbred Brown Norway (BN) rat into the background of an inbred substrain of the Dahl salt-sensitive (SS/Mcwi) rat restored renin levels and the angiogenic response after electrical stimulation. This restored response was significantly attenuated when SS-13BN/Mcwi consomic rats were treated with lisinopril or high-salt diet. The role of ANG II on this effect was confirmed by the complete restoration of skeletal muscle angiogenesis in SS/Mcwi rats infused with subpressor doses of ANG II. Congenic strains derived from the SS-13BN/Mcwi consomic were used to further verify the role of the renin gene in this response. Microvessel density was markedly increased after stimulation in congenic strains that contained the renin gene from the BN rat (congenic lines A and D). This angiogenic response was suppressed in control strains that carried regions of the BN genome just above (congenic line C) or just below (congenic line B) the renin gene. The present study emphasizes the importance of maintaining normal renin regulation as well as ANG II levels during the angiogenesis process with a combination of physiological, genetic, and pharmacological manipulation of the RAS.
- Published
- 2008
48. Time-course changes of catabolic proteins following muscle atrophy induced by dexamethasone
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Lidiane M. Souza, Aline Mio Martuscelli, Paula B. Constantino, Anderson Saranz Zago, Sandra Lia do Amaral, Anderson G. Macedo, James W. E. Rush, Andre L. O. Krug, Carlos Ferreira dos Santos, Universidade Estadual Paulista (Unesp), University of Waterloo, and Universidade de São Paulo (USP)
- Subjects
Flexor hallucis longus muscle ,0301 basic medicine ,Male ,medicine.medical_specialty ,Time Factors ,Clinical Biochemistry ,Skeletal muscle ,Muscle Proteins ,Myostatin ,030204 cardiovascular system & hematology ,Biochemistry ,Dexamethasone ,03 medical and health sciences ,0302 clinical medicine ,Endocrinology ,Internal medicine ,medicine ,Animals ,Rats, Wistar ,Glucocorticoids ,Molecular Biology ,Protein kinase B ,Pharmacology ,biology ,Catabolism ,business.industry ,Organic Chemistry ,musculoskeletal system ,Muscle atrophy ,Rats ,Muscular Atrophy ,030104 developmental biology ,medicine.anatomical_structure ,biology.protein ,Lean body mass ,MuRF-1 ,medicine.symptom ,business ,medicine.drug - Abstract
Made available in DSpace on 2018-12-11T17:23:03Z (GMT). No. of bitstreams: 0 Previous issue date: 2016-03-01 Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) This study was designed to describe the time-course changes of catabolic proteins following muscle atrophy induced by 10 days of dexamethasone (DEX). Rats underwent DEX treatment for 1, 3, 5, 7 and 10 days. Body weight (BW) and lean mass were obtained using a dual energy X-ray absorptiometry (DEXA) scan. Muscle ringer finger1 (MuRF-1), atrogin-1 and myostatin protein levels were analyzed in the tibialis anterior (TA), flexor hallucis longus (FHL) and soleus muscles. DEX treatment reduced lean mass since day-3 and reduced BW since day-5. Specific muscle weight reductions were observed after day-10 in TA (-23%) and after day-5 in FHL (-16%, -17% and -29%, for days 5, 7 and 10, respectively). In TA, myostatin protein level was 36% higher on day-5 and its values were normalized in comparison with controls on day-10. MuRF-1 protein level was increased in TA muscle from day-7 and in FHL muscle only on day-10. This study suggests that DEX-induced muscle atrophy is a dynamic process which involves important signaling factors over time. As demonstrated by DEXA scan, lean mass declines earlier than BW and this response may involve other catabolic proteins than myostatin and MuRF-1. Specifically for TA and FHL, it seems that myostatin may trigger the catabolic process, and MuRF-1 may contribute to maintain muscle atrophy. This information may support any intervention in order to attenuate the muscle atrophy during long period of treatment. Joint Graduate Program in Physiological Sciences PIPGCF UFSCar UNESP Department of Physiological Sciences Federal University of São Carlos - UFSCAR Department of Physical Education Universidade Estadual Paulista - UNESP Department of Kinesiology Faculty of Applied Health Sciences University of Waterloo Department of Biological Sciences Bauru School of Dentistry University of São Paulo USP Joint Graduate Program in Physiological Sciences PIPGCF UFSCar UNESP Department of Physiological Sciences Federal University of São Carlos - UFSCAR Department of Physical Education Universidade Estadual Paulista - UNESP CNPq: 130232/2011-4 FAPESP: 2012/03816-9 FAPESP: 2012/21820-3
- Published
- 2015
49. Functional Local Renin-Angiotensin System in Human and Rat Periodontal Tissue
- Author
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Sandra Lia do Amaral, Gustavo Pompermaier Garlet, Rubens P. Maciel, Flávio A. Faria, Eduardo B. Oliveira, Christiane Becari, Carla Renata Sipert, Isaac R. Matus, Bella Luna Colombini-Ishikiriama, Gabriela Pereira de Souza, Camila Oliveira Rodini, Ana Carolina Morandini, Carlos Ferreira dos Santos, Caio Márcio Figueiredo, Ana Paula Akashi, Maria Cristina O. Salgado, Thiago José Dionísio, Marta da Cunha Lima, Andrew S. Greene, Daniela N. Didier, Universidade de São Paulo (USP), Universidade Estadual Paulista (Unesp), and Med Coll Wisconsin
- Subjects
Adult ,Male ,Periodontium ,medicine.medical_specialty ,Gingiva ,lcsh:Medicine ,Renin-Angiotensin System ,Young Adult ,Gingivitis ,Internal medicine ,Renin ,Renin–angiotensin system ,parasitic diseases ,medicine ,Animals ,Humans ,Periodontal fiber ,Amino Acid Sequence ,Rats, Wistar ,Periodontitis ,lcsh:Science ,Cells, Cultured ,Inflammation ,Receptors, Angiotensin ,Multidisciplinary ,Chemistry ,Angiotensin II ,lcsh:R ,Captopril ,Middle Aged ,medicine.disease ,Peptide Fragments ,Losartan ,Endocrinology ,Female ,lcsh:Q ,Angiotensin I ,medicine.symptom ,Ex vivo ,PERIODONTO ,Research Article ,medicine.drug - Abstract
Made available in DSpace on 2018-11-26T17:55:38Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-08-05 Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES) University of Sao Paulo [USP] The initiation or progression of periodontitis might involve a local renin-angiotensin system (RAS) in periodontal tissue. The aim of this study was to further characterize the local RAS in human and rat periodontal tissues between healthy and periodontally-affected tissue. Components of the RAS were investigated using in vitro, ex vivo and in vivo experiments involving both human and Wistar rat periodontium. Although not upregulated when challenged with P. gingivalis-lipopolysaccharide, human gingival and periodontal ligament fibroblasts expressed RAS components. Likewise, healthy and inflamed human gingiva expressed RAS components, some of which were shown to be functional, yet no differences in expression were found between healthy and diseased gingiva. However, in inflamed tissue the immunoreactivity was greater for the AT(1)R compared to AT(2)R in fibroblasts. When compared to healthy tissue, ACE activity was increased in human gingiva from volunteers with gingivitis. Human-gingiva homogenates generated Ang II, Ang 1-9 and Ang 1-7 when incubated with precursors. In gingiva homogenates, Ang II formation from Ang I was nearly abolished only when captopril and chymostatin were combined. Ang 1-7 formation was significantly greater when human gingiva homogenates were incubated with chymostatin alone compared to incubation without any inhibitor, only captopril, or captopril and chymostatin. In rat gingiva, RAS components were also found; their expression was not different between healthy and experimentally induced periodontitis (EP) groups. However, renin inhibition (aliskiren) and an AT(1)R antagonist (losartan) significantly blocked EP-alveolar-bone loss in rats. Collectively, these data are consistent with the hypothesis that a local RAS system is not only present but is also functional in both human and rat periodontal tissue. Furthermore, blocking AT(1)R and renin can significantly prevent periodontal bone loss induced by EP in rats. Univ Sao Paulo, Dept Biol Sci, Bauru Sch Dent, Sao Paulo, Brazil Univ Sao Paulo, Sch Dent, Dept Restorat Dent, Sao Paulo, Brazil Sao Paulo State Univ, Fac Sci, Dept Phys Educ, Sao Paulo, Brazil Univ Sao Paulo, Sch Med Ribeirao Preto, Sao Paulo, Brazil Med Coll Wisconsin, Dept Physiol, Milwaukee, WI 53226 USA Sao Paulo State Univ, Fac Sci, Dept Phys Educ, Sao Paulo, Brazil FAPESP: 2004/13479-3 FAPESP: 2005/60167-0 FAPESP: 2009/53848-1 FAPESP: 2009/15372-5 FAPESP: 2010/01230-1 FAPESP: 2013/16113-9 CNPq: 473856/2010-7 CNPq: 558171/2010-9 CNPq: 303494/2013-1 CNPq: 506802/2013-2 CNPq: 480160/2013-9 CAPES: 2435/09-9 University of Sao Paulo [USP]: 08.1.2066.25.3
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- 2015
50. Tratamento das Dislipidemias com Estatinas e Exercícios Físicos: Evidências Recentes das Respostas Musculares
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Sandra Lia do Amaral, Henrique Luiz Monteiro, Mariana Rotta Bonfim, Acary Souza Bulle Oliveira, Universidade Estadual Paulista (UNESP), Universidade Federal de São Paulo (UNIFESP), Universidade Estadual Paulista (Unesp), and Universidade Federal de São Paulo Escola Paulista de Medicina Setor de Doenças Neuromusculares
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medicine.medical_specialty ,lcsh:Diseases of the circulatory (Cardiovascular) system ,Statin ,Exacerbation ,medicine.drug_class ,Exercício ,Physical exercise ,Review Article ,Hydroxymethylglutaryl-CoA reductase inhibitors ,Muscular Diseases ,Musculoskeletal Pain ,Músculo Esquelético ,Medicine ,Aerobic exercise ,Eccentric ,Humans ,Creatine Kinase ,Exercise ,Dyslipidemias ,biology ,business.industry ,Muscle, skeletal ,Skeletal muscle ,Inibidores de Hidroximetilglutaril-CoA Redutases ,medicine.disease ,Exercise Therapy ,medicine.anatomical_structure ,Dislipidemias / terapia ,Dyslipidemia ,lcsh:RC666-701 ,Physical therapy ,biology.protein ,Creatine kinase ,Hydroxymethylglutaryl-CoA Reductase Inhibitors ,Cardiology and Cardiovascular Medicine ,business - Abstract
Made available in DSpace on 2015-08-06T16:14:39Z (GMT). No. of bitstreams: 0 Previous issue date: 2015-04-01. Added 1 bitstream(s) on 2015-08-06T16:44:32Z : No. of bitstreams: 1 S0066-782X2015000400009-pt.pdf: 293433 bytes, checksum: 2e2e465b814fb503717fa96783f0b6d5 (MD5). Added 1 bitstream(s) on 2015-08-06T16:44:32Z : No. of bitstreams: 2 S0066-782X2015000400009-pt.pdf: 293433 bytes, checksum: 2e2e465b814fb503717fa96783f0b6d5 (MD5) S0066-782X2015000400009-en.pdf: 271141 bytes, checksum: f9fda24fbd4507ad1746ece81c771070 (MD5) Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq) A associação do tratamento medicamentoso por estatinas com a prática de exercícios físicos pode reduzir substancialmente o risco de mortalidade cardiovascular de indivíduos dislipidêmicos, porém sua realização vem sendo associada à exacerbação de quadros miopáticos. O presente trabalho teve como objetivo apresentar os resultados mais recentes da literatura específica sobre os efeitos da associação de estatinas ao exercício físico na musculatura esquelética. Para tanto, realizou-se levantamento da literatura nas bases de dados PubMed e SciELO, utilizando a combinação dos unitermos: “estatina/estatinas” AND “exercício” AND “músculo” (“statin” AND “exercise” AND “muscle”), sendo selecionados apenas artigos originais publicados entre janeiro de 1990 e novembro de 2013. Foram analisados 16 artigos que avaliaram o efeito da associação das estatinas com exercício agudo ou crônico na musculatura esquelética. Os resultados dos estudos apontaram que atletas podem experimentar efeitos deletérios na musculatura esquelética quando do uso de estatinas, visto que os quadros de exacerbação da lesão muscular pelo exercício foram mais frequentes com treinamento intenso ou exercícios agudos excêntricos e extenuantes. O treinamento físico moderado, por sua vez, quando associado às estatinas, não aumenta os relatos de dor nem os níveis de creatina quinase, além de acarretar ganhos nas funções musculares e metabólicas advindas do treinamento. Sugere-se, portanto, que pacientes dislipidêmicos em tratamento com estatinas sejam expostos ao treinamento físico aeróbio combinado a exercícios resistidos, de intensidade moderada, em três sessões semanais, sendo que a oferta do treinamento físico previamente à administração do tratamento medicamentoso, quando possível, faz-se desejável. Statin treatment in association with physical exercise practice can substantially reduce cardiovascular mortality risk of dyslipidemic individuals, but this practice is associated with myopathic event exacerbation. This study aimed to present the most recent results of specific literature about the effects of statins and its association with physical exercise on skeletal musculature. Thus, a literature review was performed using PubMed and SciELO databases, through the combination of the keywords “statin” AND “exercise” AND “muscle”, restricting the selection to original studies published between January 1990 and November 2013. Sixteen studies evaluating the effects of statins in association with acute or chronic exercises on skeletal muscle were analyzed. Study results indicate that athletes using statins can experience deleterious effects on skeletal muscle, as the exacerbation of skeletal muscle injuries are more frequent with intense training or acute eccentric and strenuous exercises. Moderate physical training, in turn, when associated to statins does not increase creatine kinase levels or pain reports, but improves muscle and metabolic functions as a consequence of training. Therefore, it is suggested that dyslipidemic patients undergoing statin treatment should be exposed to moderate aerobic training in combination to resistance exercises three times a week, and the provision of physical training prior to drug administration is desirable, whenever possible. Universidade Estadual Paulista Instituto de Biociências Programa de Pós-Graduação em Ciências da Motricidade Universidade Federal de São Paulo Escola Paulista de Medicina Setor de Doenças Neuromusculares Universidade Estadual Paulista Faculdade de Ciências Departamento de Educação Física Universidade Estadual Paulista Instituto de Biociências Programa de Pós-Graduação em Ciências da Motricidade Universidade Estadual Paulista Faculdade de Ciências Departamento de Educação Física
- Published
- 2015
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