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Influence of training status and eNOS haplotypes on plasma nitrite concentrations in normotensive older adults: a hypothesis-generating study
- Source :
- Repositório Institucional da USP (Biblioteca Digital da Produção Intelectual), Universidade de São Paulo (USP), instacron:USP
- Publication Year :
- 2014
- Publisher :
- Springer Science and Business Media LLC, 2014.
-
Abstract
- The purpose of this study was to evaluate the relationship between 3 eNOS gene polymorphisms and training status (TS) in affecting plasma nitrite concentration (NO2) in normotensive adults over 50 years old. Resting blood pressure (BP) was measured in all participants (n = 101). Plasma was taken to analyze: lipid profile, nitrite concentration (NO2) and lipid peroxide levels (T-BARS). Also, genomic DNA was extracted from plasma for genotyping NOS3 polymorphisms (-786T>C; 894G>T; and VNTR in intron 4). TS was determined by one-mile walk test and Functional Fitness Test Battery from AAHPERD (TS1—regular TS; TS2—good TS; and TS3—very good TS). BP was not influenced by TS, but NO2 was 15 % higher in TS3 (123 ± 27 nM) compared to TS-2 (106 ± 22 nM). No differences were found in plasma NO2 in the haplotype analyses. However, the presence of the C allele (T-786C) and ASP allele (Glu298Asp) was found to enhance the correlation between TS and NO2 levels (r = 0.492 in C/4b/ASP haplotype and r = 0.855 in C/4a/ASP haplotype). This study thus identifies NOS3 polymorphism-dependent sensitivity to the effects of physical training on plasma NO2. Maintenance of good levels of training status, in carriers of C allele for T-786C polymorphism, combined with ASP allele for Glu298Asp polymorphism, may result in an increase in the NO2 plasma concentrations, which may reflect improved NO bioavailability in older adult normotensive individuals.
- Subjects :
- Aging
medicine.medical_specialty
Nitric Oxide Synthase Type III
Blood Pressure
Biology
chemistry.chemical_compound
Polymorphism (computer science)
Enos
Internal medicine
ÓXIDO NÍTRICO
medicine
Humans
Allele
Nitrite
Exercise
Genotyping
Alleles
Nitrites
Aged
Polymorphism, Genetic
Lipid peroxide
medicine.diagnostic_test
Teaching
Haplotype
biology.organism_classification
Surgery
Endocrinology
Haplotypes
chemistry
Geriatrics and Gerontology
Lipid profile
Subjects
Details
- ISSN :
- 17208319
- Volume :
- 26
- Database :
- OpenAIRE
- Journal :
- Aging Clinical and Experimental Research
- Accession number :
- edsair.doi.dedup.....eb5a1b73b3503b6c6ac4e41b5895c6d7