29 results on '"Mei-Zhen Zou"'
Search Results
2. Cytomembrane nanovaccines show therapeutic effects by mimicking tumor cells and antigen presenting cells
- Author
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Wen-Long Liu, Mei-Zhen Zou, Tao Liu, Jin-Yue Zeng, Xue Li, Wu-Yang Yu, Chu-Xin Li, Jing-Jie Ye, Wen Song, Jun Feng, and Xian-Zheng Zhang
- Subjects
Science - Abstract
Cancer vaccines often fail to generate clinically relevant effects. Here, the authors generate a nanosized cytomembrane vaccine based on fusion between dendritic cells and cancer cells, and show them to activate anti-tumor immune responses via their antigen presenting and T-cell activating functions.
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- 2019
- Full Text
- View/download PDF
3. A nanodevice with lifetime-improved singlet oxygen for enhanced photodynamic therapy
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A-Min Cao, Wen-Long Liu, Mei-Zhen Zou, Si-Yong Qin, Yin-Jia Cheng, and Ai-Qing Zhang
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Photosensitizing Agents ,Photochemotherapy ,Singlet Oxygen ,Materials Chemistry ,Metals and Alloys ,Ceramics and Composites ,General Chemistry ,Catalysis ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials - Abstract
The short lifetime of singlet oxygen reduces its accumulation in the endoplasmic reticulum, which limits the output of photodynamic therapy. A nanodevice with functions of singlet oxygen production, storage and release can improve the lifetime of singlet oxygen for enhancing phototherapeutic efficacy.
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- 2022
4. Homotypic Targeted Photosensitive Nanointerferer for Tumor Cell Cycle Arrest to Boost Tumor Photoimmunotherapy
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Xue-Feng Bai, Ying Chen, Mei-Zhen Zou, Chu-Xin Li, Yu Zhang, Min-Jie Li, Si-Xue Cheng, and Xian-Zheng Zhang
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Mice ,Cell Line, Tumor ,Colonic Neoplasms ,General Engineering ,Tumor Microenvironment ,General Physics and Astronomy ,Animals ,General Materials Science ,Immunotherapy ,Cell Cycle Checkpoints ,CD8-Positive T-Lymphocytes - Abstract
Recent advances in tumor immunotherapy mainly tend to remodel the immunosuppressive tumor microenvironment (TME) for immune enhancement. However, the complexity of TME makes it unlikely to achieve satisfactory therapeutic effects with any single intervention alone. Here, we focus on exposing intrinsic features of tumor cells to trigger direct pleiotropic antitumor immunity. We develop a photosensitive nanointerferer that is engineered with a nanoscale metal-organic framework decorated with tumor cell membranes for targeted delivery of a photosensitizer and small interfering RNA, which is used to knock down cyclin-dependent kinase 4 (Cdk4). Cdk4 blockade can arrest the cell cycle of tumor cells to facilitate antigen exposure and increase the expression level of programmed cell death protein ligand 1 (PD-L1). Under laser irradiation, photodynamic damage triggered by the nanointerferer induces the release of tumor antigens and recruitment of dendritic cells (DCs), thereby promoting the antitumor activity of CD8
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- 2022
5. Near-Infrared Light Responsive Nanoreactor for Simultaneous Tumor Photothermal Therapy and Carbon Monoxide-Mediated Anti-Inflammation
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Cheng Zhang, Shi-Bo Wang, Chuanjun Liu, Mei-Zhen Zou, Jing-Jie Ye, and Xian-Zheng Zhang
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010405 organic chemistry ,General Chemical Engineering ,technology, industry, and agriculture ,General Chemistry ,Nanoreactor ,Polyethylene glycol ,Photothermal therapy ,010402 general chemistry ,Photochemistry ,01 natural sciences ,0104 chemical sciences ,Chemistry ,chemistry.chemical_compound ,chemistry ,PEG ratio ,medicine ,Photocatalysis ,Ferric ,Irradiation ,QD1-999 ,Research Article ,Carbon monoxide ,medicine.drug - Abstract
Photothermal therapy (PTT) is an effective treatment modality with high selectivity for tumor suppression. However, the inflammatory responses caused by PTT may lead to adverse reactions including tumor recurrence and therapeutic resistance, which are regarded as major problems for PTT. Here, a near-infrared (NIR) light-responsive nanoreactor (P@DW/BC) is fabricated to simultaneously realize tumor PTT and carbon monoxide (CO)-mediated anti-inflammatory therapy. Defective tungsten oxide (WO3) nanosheets (DW NSs) are decorated with bicarbonate (BC) via ferric ion-mediated coordination and then modified with polyethylene glycol (PEG) on the surface to fabricate PEG@DW/BC or P@DW/BC nanosheets. Upon 808 nm NIR laser irradiation, the DW content in P@DW/BC can serve as not only a photothermal agent to realize photothermal conversion but also a photocatalyst to convert carbon dioxide (CO2) to CO. In particular, the generated heat can also trigger the decomposition of BC to produce CO2 near the NSs, thus enhancing the photocatalytic CO generation. Benefiting from the efficient hyperthermia and CO generation under single NIR laser irradiation, P@DW/BC can realize effective thermal ablation of tumor and simultaneous inhibition of PTT-induced inflammation., A near-infrared light-responsive nanoreactor with photothermal conversion and carbon monoxide (CO) production was fabricated for tumor photothermal therapy and CO-mediated anti-inflammation.
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- 2020
6. A nanodevice with lifetime-improved singlet oxygen for enhanced photodynamic therapy
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Cao, Amin, primary, Liu, Wenlong, additional, Mei-Zhen, Zou, additional, Qin, Si-Yong, additional, Cheng, Yin-Jia, additional, and Zhang, Ai-Qing, additional
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- 2022
- Full Text
- View/download PDF
7. Hybrid Vesicles Based on Autologous Tumor Cell Membrane and Bacterial Outer Membrane To Enhance Innate Immune Response and Personalized Tumor Immunotherapy
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Xue-Feng Bai, Mei-Zhen Zou, Zi-Hao Li, Chuanjun Liu, and Xian-Zheng Zhang
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Innate immune system ,Bacterial outer membrane vesicles ,Chemistry ,Mechanical Engineering ,medicine.medical_treatment ,T-Lymphocytes ,Cell Membrane ,Bioengineering ,General Chemistry ,Immunotherapy ,Condensed Matter Physics ,Acquired immune system ,medicine.disease ,Autologous tumor cell ,Immunity, Innate ,Metastasis ,Immune system ,Bacterial Outer Membrane ,Antigen ,Cancer research ,medicine ,General Materials Science - Abstract
Tumor heterogeneity, often leading to metastasis, limits the development of tumor therapy. Personalized therapy is promising to address tumor heterogeneity. Here, a vesicle system was designed to enhance innate immune response and amplify personalized immunotherapy. Briefly, the bacterial outer membrane vesicle (OMV) was hybridized with the cell membrane originated from the tumor (mT) to form new functional vesicles (mTOMV). In vitro experiments revealed that the mTOMV strengthened the activation of innate immune cells and increased the specific lysis ability of T cells in homogeneous tumors. In vivo experiments showed that the mTOMV effectively accumulated in inguinal lymph nodes, then inhibited lung metastasis. Besides, the mTOMV evoked adaptive immune response in homologous tumor rather than the heterogeneous tumor, reversibly demonstrating the effects of personalized immunotherapy. The functions to inhibit tumor growth and metastasis accompanying good biocompatibility and simple preparation procedure of mTOMV provide their great potential for clinical applications.
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- 2021
8. A near infrared ratiometric platform based π-extended porphyrin metal-organic framework for O
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Bo-Ru, Xie, Yun, Yu, Xin-Hua, Liu, Jin-Yue, Zeng, Mei-Zhen, Zou, Chu-Xin, Li, Xuan, Zeng, and Xian-Zheng, Zhang
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Oxygen ,Photosensitizing Agents ,Porphyrins ,Photochemotherapy ,Cell Line, Tumor ,Neoplasms ,Humans ,Nanoparticles ,Metal-Organic Frameworks - Abstract
Photodynamic therapy (PDT) is widely researched in tumor treatment, but its therapeutic effect is affected by oxygen (O
- Published
- 2021
9. PLA-PEG Micelles Loaded with a Classic Vasodilator for Oxidative Cataract Prevention
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Yun-Xia Sun, Wen-Long Liu, Lu Xu, Chi Zhang, Mei-Zhen Zou, Wen-Xiu Qiu, and Xian-Zheng Zhang
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genetic structures ,Biocompatibility ,medicine.drug_class ,medicine.medical_treatment ,0206 medical engineering ,Biomedical Engineering ,chemistry.chemical_element ,macromolecular substances ,02 engineering and technology ,Calcium channel blocker ,Calcium ,Pharmacology ,medicine.disease_cause ,Micelle ,Biomaterials ,Nifedipine ,medicine ,technology, industry, and agriculture ,Eye drop ,021001 nanoscience & nanotechnology ,020601 biomedical engineering ,eye diseases ,Bioavailability ,chemistry ,sense organs ,0210 nano-technology ,Oxidative stress ,medicine.drug - Abstract
The only treatment for cataract in clinic is the clouded lens removal combined with artificial lens implantation. In this study, nifedipine (NFP), a classic vasodilator, was loaded in a U.S. FDA-approved polymer PLA–PEG to form NFP-loaded PLA–PEG micelles as a novel eye drop to prevent oxidative cataract formation and progression at the early stage. The NFP-loaded PLA–PEG micelles not only showed satisfactory biocompatibility and bioavailability, but also efficiently improved the anticataract ability through the inhibition of extracellular calcium ions influx. This study may provide a new insight into the development of cataract treatment.
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- 2021
10. Advances in nanomaterials for treatment of hypoxic tumor
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Xian-Zheng Zhang, Mei-Zhen Zou, Xue-Feng Bai, Jing-Jie Ye, Wen-Long Liu, Han-Shi Chen, Han Cheng, and Fan Gao
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AcademicSubjects/SCI00010 ,medicine.medical_treatment ,Materials Science ,02 engineering and technology ,Review ,010402 general chemistry ,01 natural sciences ,Metastasis ,Nanomaterials ,medicine ,nanomaterials ,Chemotherapy ,Multidisciplinary ,Hypoxic tumor ,business.industry ,High mortality ,Tumor therapy ,Immunotherapy ,hypoxic tumor ,021001 nanoscience & nanotechnology ,medicine.disease ,0104 chemical sciences ,Radiation therapy ,Cancer research ,tumor therapy ,0210 nano-technology ,business ,AcademicSubjects/MED00010 ,oxygen - Abstract
The hypoxic tumor microenvironment is characterized by disordered vasculature and rapid proliferation of tumors, resulting from tumor invasion, progression and metastasis. The hypoxic conditions restrict efficiency of tumor therapies, such as chemotherapy, radiotherapy, phototherapy and immunotherapy, leading to serious results of tumor recurrence and high mortality. Recently, research has concentrated on developing functional nanomaterials to treat hypoxic tumors. In this review, we categorize such nanomaterials into (i) nanomaterials that elevate oxygen levels in tumors for enhanced oxygen-dependent tumor therapy and (ii) nanomaterials with diminished oxygen dependence for hypoxic tumor therapy. To elevate oxygen levels in tumors, oxygen-carrying nanomaterials, oxygen-generating nanomaterials and oxygen-economizing nanomaterials can be used. To diminish oxygen dependence of nanomaterials for hypoxic tumor therapy, therapeutic gas-generating nanomaterials and radical-generating nanomaterials can be used. The biocompatibility and therapeutic efficacy of these nanomaterials are discussed.
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- 2020
11. A MSN-based tumor-targeted nanoplatform to interfere with lactate metabolism to induce tumor cell acidosis for tumor suppression and anti-metastasis
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Zhao-Xia Chen, Xian-Zheng Zhang, Deng-Ke Guo, Mei-Zhen Zou, Zhenlin Zhong, Miao-Deng Liu, Shi-Bo Wang, and Han Cheng
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Silicon ,Fluvastatin Sodium ,Antineoplastic Agents ,Metastasis ,03 medical and health sciences ,0302 clinical medicine ,Folic Acid ,Cell Line, Tumor ,Neoplasms ,Extracellular ,medicine ,Tumor Microenvironment ,Humans ,General Materials Science ,Neoplasm Metastasis ,Fluvastatin ,030304 developmental biology ,Acidosis ,0303 health sciences ,Tumor microenvironment ,Chemistry ,medicine.disease ,Metformin ,Manganese Compounds ,Cell culture ,030220 oncology & carcinogenesis ,Cancer research ,Lactates ,Nanoparticles ,Efflux ,medicine.symptom ,Porosity ,medicine.drug - Abstract
Lactate, the main contributor to the acidic tumor microenvironment, not only promotes the proliferation of tumor cells, but also closely relates to tumor invasion and metastasis. Here, a tumor targeting nanoplatform, designated as Me&Flu@MSN@MnO2-FA, was fabricated for effective tumor suppression and anti-metastasis by interfering with lactate metabolism of tumor cells. Metformin (Me) and fluvastatin sodium (Flu) were incorporated into MnO2-coated mesoporous silicon nanoparticles (MSNs), the synergism between Me and Flu can modulate the pyruvate metabolic pathway to produce more lactate, and concurrently inhibit lactate efflux to induce intracellular acidosis to kill tumor cells. As a result of the restricted lactate efflux, the extracellular lactate concentration is reduced, and the ability of the tumor cells to migrate is also weakened. This ingenious strategy based on Me&Flu@MSN@MnO2-FA showed an obvious inhibitory effect on tumor growth and resistance to metastasis.
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- 2020
12. π-Extended Benzoporphyrin-Based Metal–Organic Framework for Inhibition of Tumor Metastasis
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Xuan Zeng, Xiao-Shuang Wang, Xian-Zheng Zhang, Hengjiang Cong, Mei-Zhen Zou, Ming-Kang Zhang, and Jin-Yue Zeng
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medicine.medical_treatment ,Programmed Cell Death 1 Receptor ,genetic processes ,General Physics and Astronomy ,Apoptosis ,Photodynamic therapy ,02 engineering and technology ,01 natural sciences ,B7-H1 Antigen ,Polyethylene Glycols ,Metastasis ,Mice ,chemistry.chemical_compound ,Cancer immunotherapy ,Tumor Microenvironment ,Tissue Distribution ,General Materials Science ,Photosensitizer ,Neoplasm Metastasis ,Metal-Organic Frameworks ,Mice, Inbred BALB C ,Photosensitizing Agents ,Singlet Oxygen ,General Engineering ,021001 nanoscience & nanotechnology ,Combined Modality Therapy ,Primary tumor ,Heterografts ,Immunotherapy ,0210 nano-technology ,Porphyrins ,Cell Survival ,Antineoplastic Agents ,macromolecular substances ,010402 general chemistry ,Cell Line, Tumor ,medicine ,Animals ,Humans ,fungi ,medicine.disease ,Porphyrin ,0104 chemical sciences ,enzymes and coenzymes (carbohydrates) ,Photochemotherapy ,chemistry ,health occupations ,Cancer research ,Nanoparticles ,Zirconium ,Ethylene glycol - Abstract
We report on the benzoporphyrin-based metal–organic framework (TBP-MOF), with 10-connected Zr6 cluster and much improved photophysical properties over the traditional porphyrin-based MOFs. It was found that TBP-MOF exhibited red-shifted absorption bands and strong near-infrared luminescence for bioimaging, whereas the π-extended benzoporphyrin-based linkers of TBP-MOF facilitated 1O2 generation to enhance O2-dependent photodynamic therapy (PDT). It was demonstrated that poly(ethylene glycol)-modified nanoscale TBP-MOF (TBP-nMOF) can be used as an effective PDT agent under hypoxic tumor microenvironment. We also elucidated that the low O2-dependent PDT of TBP-nMOF in combination with αPD-1 checkpoint blockade therapy can not only suppress the growth of primary tumor, but also stimulate an antitumor immune response for inhibiting metastatic tumor growth. We believe this TBP-nMOF has great potential to serve as an efficient photosensitizer for PDT and cancer immunotherapy.
- Published
- 2018
13. A near infrared ratiometric platform based π-extended porphyrin metal-organic framework for O2 imaging and cancer therapy
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Xian-Zheng Zhang, Xin-Hua Liu, Chu-Xin Li, Xuan Zeng, Bo-Ru Xie, Yun Yu, Jin-Yue Zeng, and Mei-Zhen Zou
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0303 health sciences ,Singlet oxygen ,medicine.medical_treatment ,Near-infrared spectroscopy ,Biophysics ,Cancer ,Nanoprobe ,Bioengineering ,Photodynamic therapy ,02 engineering and technology ,021001 nanoscience & nanotechnology ,medicine.disease ,Porphyrin ,Biomaterials ,03 medical and health sciences ,chemistry.chemical_compound ,chemistry ,Mechanics of Materials ,Ceramics and Composites ,medicine ,Immunogenic cell death ,Metal-organic framework ,0210 nano-technology ,030304 developmental biology - Abstract
Photodynamic therapy (PDT) is widely researched in tumor treatment, but its therapeutic effect is affected by oxygen (O2) concentration of tumor site. Here, we developed a Pd-coordinated π-conjugated extended porphyrin doped porphyrin metal-organic-framework (named as PTP). PTP can achieve near infrared (NIR) O2 concentration ratiometric imaging, solving the problems of short detection wavelengths and influence of self-concentrations. With the NIR excitation wavelength and the ability of higher singlet oxygen (1O2) generation, PTP can induce PDT more effectively. The efficient PDT also mediates cancer immunogenic cell death (ICD), which combines with the immune checkpoint inhibitor αPD-1 to achieve obviously cancer suppression and anti-metastasis effect. This theranostic NIR ratiometric nanoprobe can be used as a pre-evaluation on the outcome of PDT and high-efficient cancer combined treatment system, which will find great potential in tumor diagnosis and treatment.
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- 2021
14. Remodeling extracellular matrix based on functional covalent organic framework to enhance tumor photodynamic therapy
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Fan Gao, Xian-Zheng Zhang, Cheng Zhang, Zhao-Xia Chen, Jing-Jie Ye, Shi-Bo Wang, Mei-Zhen Zou, and Xuan Zeng
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medicine.medical_treatment ,Biophysics ,Bioengineering ,Photodynamic therapy ,02 engineering and technology ,Biomaterials ,Extracellular matrix ,03 medical and health sciences ,chemistry.chemical_compound ,In vivo ,Cell Line, Tumor ,Neoplasms ,Hyaluronic acid ,medicine ,Humans ,Photosensitizer ,Metal-Organic Frameworks ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Reactive oxygen species ,Photosensitizing Agents ,Tumor hypoxia ,Chemistry ,021001 nanoscience & nanotechnology ,Extracellular Matrix ,Photochemotherapy ,Mechanics of Materials ,Ceramics and Composites ,Nanoparticles ,0210 nano-technology ,Ethylene glycol - Abstract
Photodynamic therapy (PDT) is a promising treatment modality for tumor suppression. However, the hypoxic state of most solid tumors might largely hinder the efficacy of PDT. Here, a functional covalent organic framework (COF) is fabricated to enhance PDT efficacy by remodeling the tumor extracellular matrix (ECM). Anti-fibrotic drug pirfenidone (PFD) is loaded in an imine-based COF (COFTTA-DHTA) and followed by the decoration of poly(lactic-co-glycolic-acid)-poly(ethylene glycol) (PLGA-PEG) to fabricate PFD@COFTTA-DHTA@PLGA-PEG, or PCPP. After injected intravenously, PCPP can accumulate and release PFD in tumor sites, leading to down-regulation of ECM compenents such as hyaluronic acid (HA) and collagen I. Such depletion of tumor ECM reduces the intratumoral solid stress, a compressive force exerted by the ECM and cells, decompresses tumor blood vessels, and increases the density of effective vascular areas, resulting in significantly improved oxygen supply in tumor. Furthermore, PCPP-mediated tumor ECM depletion also enhances the tumor uptake of subsequently injected Protoporphyrinl IX (PPIX)-conjugated peptide formed nanomicelles (NM-PPIX) due to the improved enhanced permeability and retention (EPR) effect. Both the alleviated tumor hypoxia and improved tumor homing of photosensitizer (PS) molecules after PCPP treatment significantly increase the reactive oxygen species (ROS) generation in tumor and therefore realize greatly enhanced PDT effect of tumor in vivo.
- Published
- 2019
15. Cytomembrane nanovaccines show therapeutic effects by mimicking tumor cells and antigen presenting cells
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Chu-Xin Li, Xian-Zheng Zhang, Jun Feng, Wen-Long Liu, Mei-Zhen Zou, Tao Liu, Jing-Jie Ye, Wu-Yang Yu, Wen Song, Jin-Yue Zeng, and Xue Li
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0301 basic medicine ,Science ,T-Lymphocytes ,T cell ,medicine.medical_treatment ,Transplantation, Heterologous ,General Physics and Astronomy ,02 engineering and technology ,Biology ,Lymphocyte Activation ,Cancer Vaccines ,Article ,General Biochemistry, Genetics and Molecular Biology ,Cell Fusion ,Mice ,03 medical and health sciences ,Antigen ,Antigens, Neoplasm ,Nanoscience and technology ,Cell Line, Tumor ,medicine ,Animals ,lcsh:Science ,Antigen-presenting cell ,Cancer ,Antigen Presentation ,Mice, Inbred BALB C ,Multidisciplinary ,Cell fusion ,Cell Membrane ,Mammary Neoplasms, Experimental ,Dendritic Cells ,General Chemistry ,Immunotherapy ,021001 nanoscience & nanotechnology ,Tumor antigen ,030104 developmental biology ,medicine.anatomical_structure ,Cell culture ,Cancer cell ,Cancer research ,Nanoparticles ,lcsh:Q ,Female ,Transcriptome ,0210 nano-technology - Abstract
Most cancer vaccines are unsuccessful in eliciting clinically relevant effects. Without using exogenous antigens and adoptive cells, we show a concept of utilizing biologically reprogrammed cytomembranes of the fused cells (FCs) derived from dendritic cells (DCs) and cancer cells as tumor vaccines. The fusion of immunologically interrelated two types of cells results in strong expression of the whole tumor antigen complexes and the immunological co-stimulatory molecules on cytomembranes (FMs), allowing the nanoparticle-supported FM (NP@FM) to function like antigen presenting cells (APCs) for T cell immunoactivation. Moreover, tumor-antigen bearing NP@FM can be bio-recognized by DCs to induce DC-mediated T cell immunoactivation. The combination of these two immunoactivation pathways offers powerful antitumor immunoresponse. Through mimicking both APCs and cancer cells, this cytomembrane vaccine strategy can develop various vaccines toward multiple tumor types and provide chances for accommodating diverse functions originating from the supporters., Cancer vaccines often fail to generate clinically relevant effects. Here, the authors generate a nanosized cytomembrane vaccine based on fusion between dendritic cells and cancer cells, and show them to activate anti-tumor immune responses via their antigen presenting and T-cell activating functions.
- Published
- 2019
16. Artificial Natural Killer Cells for Specific Tumor Inhibition and Renegade Macrophage Re-Education
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Xue-Feng Bai, Xian-Zheng Zhang, Fan Gao, Han-Shi Chen, Wen-Long Liu, Mei-Zhen Zou, and Xuan Zeng
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Chemokine ,Materials science ,medicine.medical_treatment ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Glucose Oxidase ,Mice ,Immune system ,In vivo ,Biomimetics ,Cell Line, Tumor ,medicine ,Tumor Microenvironment ,Macrophage ,Animals ,General Materials Science ,Tumor microenvironment ,biology ,Mechanical Engineering ,Macrophages ,Immunotherapy ,Hydrogen Peroxide ,021001 nanoscience & nanotechnology ,In vitro ,0104 chemical sciences ,Killer Cells, Natural ,Red blood cell ,medicine.anatomical_structure ,Glucose ,Mechanics of Materials ,Cancer research ,biology.protein ,Cytokines ,0210 nano-technology - Abstract
Natural killer (NK) cells can not only recognize and eliminate abnormal cells but also recruit and re-educate immune cells to protect the host. However, the functions of NK cells are often limited in the immunosuppressive tumor microenvironment (TME). Here, artificial NK cells (designated as aNK) with minor limitations of TME for specific tumor killing and renegade macrophage re-education are created. The red blood cell membrane (RBCM) cloaks perfluorohexane (PFC) and glucose oxidase (GOX) to construct the aNK. The aNK can directly kill tumor cells by exhausting glucose and generating hydrogen peroxide (H2 O2 ). The generated H2 O2 is also similar to cytokines and chemokines for recruiting immune cells and re-educating survived macrophages to attack tumor cells. In addition, the oxygen-carried PFC can strengthen the catalytic reaction of GOX and normalize the hypoxic TME. In vitro and in vivo experiments display that aNK with slight TME limitations exhibit efficient tumor inhibition and immune activation. The aNK will provide a new sight to treat tumor as the supplement of aggressive NK cells.
- Published
- 2019
17. Nanoparticles from Cuttlefish Ink Inhibit Tumor Growth by Synergizing Immunotherapy and Photothermal Therapy
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Rong-Hui Deng, Xian-Zheng Zhang, Si-Yuan Peng, Di-Wei Zheng, Han-Shi Chen, Wen-Long Liu, Mei-Zhen Zou, Yun-Xia Sun, Xue-Feng Bai, and Pang-Hu Zhou
- Subjects
Indoles ,medicine.medical_treatment ,General Physics and Astronomy ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Mice ,Immune system ,In vivo ,Cell Line, Tumor ,Neoplasms ,medicine ,Cytotoxic T cell ,Macrophage ,Animals ,Humans ,General Materials Science ,Cell Proliferation ,Chemistry ,Macrophages ,Photothermal effect ,General Engineering ,Decapodiformes ,Immunotherapy ,Hyperthermia, Induced ,Photothermal therapy ,Phototherapy ,021001 nanoscience & nanotechnology ,medicine.disease ,Primary tumor ,0104 chemical sciences ,Cancer research ,Nanoparticles ,Ink ,0210 nano-technology ,T-Lymphocytes, Cytotoxic - Abstract
Natural nanoparticles have been extensively studied due to their diverse properties and easy accessibility. Here, the nanoparticles extracted from cuttlefish ink (CINPs) with significant antitumor efficacy are explored. These CINPs, with spherical morphology, good dispersibility, and biocompatibility, are rich in melanin and contain a variety of amino acids and monosaccharides. Through the activation of mitogen-activated protein kinase (MAPK) signaling pathway, CINPs can efficiently reprogram tumor-associated macrophages (TAMs) from immune-suppressive M2-like phenotype to antitumor M1-like phenotype. Besides, under near-infrared (NIR) irradiation, CINPs exhibit high photothermal effect and tumor cell killing ability, which make them a potential candidate in photothermal therapy (PTT) of tumor. In vivo, CINPs can increase the proportion of M1 macrophages and foster the recruitment of cytotoxic T lymphocytes (CTLs) to tumors, leading to reduced primary tumor growth and lung metastasis. In combination with their photothermal effect, which can induce tumor-specific antigens release, CINPs could almost completely inhibit tumor growth accompanied by more active immune responses. Collectively, these CINPs described here can provide both tumor immunotherapy and PTT, implying that CINPs are promising for tumor treatment.
- Published
- 2019
18. Expandable Immunotherapeutic Nanoplatforms Engineered from Cytomembranes of Hybrid Cells Derived from Cancer and Dendritic Cells
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Wen Song, Xue Li, Wu-Yang Yu, Xian-Zheng Zhang, Wen-Long Liu, Mei-Zhen Zou, Jun Feng, Chu-Xin Li, Tao Liu, Jing-Jie Ye, and Jin-Yue Zeng
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Materials science ,Porphyrins ,medicine.medical_treatment ,Transplantation, Heterologous ,Mice, Nude ,Photodynamic therapy ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Antibodies ,Mice ,Antigen ,Cell Line, Tumor ,Neoplasms ,medicine ,Animals ,General Materials Science ,Multiple tumors ,Metal-Organic Frameworks ,Cell fusion ,Photosensitizing Agents ,Mechanical Engineering ,Cell Membrane ,Histocompatibility Antigens Class II ,Cancer ,Immunotherapy ,Dendritic Cells ,021001 nanoscience & nanotechnology ,medicine.disease ,0104 chemical sciences ,Nanostructures ,Hyaluronan Receptors ,Photochemotherapy ,Mechanics of Materials ,Cancer cell ,Cancer research ,Zirconium ,0210 nano-technology ,Immunoresponse - Abstract
Using the cytomembranes (FMs) of hybrid cells acquired from the fusion of cancer and dendritic cells (DCs), this study offers a biologically derived platform for the combination of immunotherapy and traditional oncotherapy approaches. Due to the immunoactivation implicated in the cellular fusion, FMs can effectively express whole cancer antigens and immunological co-stimulatory molecules for robust immunotherapy. FMs share the tumor's self-targeting character with the parent cancer cells. In bilateral tumor-bearing mouse models, the FM-coated nanophotosensitizer causes durable immunoresponse to inhibit the rebound of primary tumors post-nanophotosensitizer-induced photodynamic therapy (PDT). The FM-induced immunotherapy displays ultrahigh antitumor effects even comparable to that of PDT. On the other hand, PDT toward primary tumors enhances the immunotherapy-caused regression of the irradiation-free distant tumors. Consequently, both the primary and the distant tumors are almost completely eliminated. This tumor-specific immunotherapy-based nanoplatform is potentially expandable to multiple tumor types and readily equipped with diverse functions owing to the flexible nanoparticle options.
- Published
- 2019
19. Hybrid Vesicles Based on Autologous Tumor Cell Membrane and Bacterial Outer Membrane To Enhance Innate Immune Response and Personalized Tumor Immunotherapy.
- Author
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Mei-Zhen Zou, Zi-Hao Li, Xue-Feng Bai, Chuan-Jun Liu, and Xian-Zheng Zhang
- Published
- 2021
- Full Text
- View/download PDF
20. Highly Integrated Nano-Platform for Breaking the Barrier between Chemotherapy and Immunotherapy
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Xian-Zheng Zhang, Lei Rong, Si-Xue Cheng, Bin Li, Jing-Yi Zhu, Jin-Xuan Fan, Di-Wei Zheng, Jia-Li Chen, Mei-Zhen Zou, Cao Li, Zushun Xu, and Qi Lei
- Subjects
medicine.medical_treatment ,Bioengineering ,02 engineering and technology ,Pharmacology ,010402 general chemistry ,01 natural sciences ,Theranostic Nanomedicine ,Metastasis ,Immune system ,Cell Line, Tumor ,Humans ,Medicine ,General Materials Science ,Doxorubicin ,Cytotoxicity ,Triple-negative breast cancer ,Chemotherapy ,business.industry ,Mechanical Engineering ,General Chemistry ,Immunotherapy ,Dendritic cell ,Silicon Dioxide ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,medicine.disease ,0104 chemical sciences ,Cancer research ,Nanoparticles ,0210 nano-technology ,business ,medicine.drug - Abstract
Fighting metastasis is a major challenge in cancer therapy, and stimulation of the immune system is of particular importance in the treatment of metastatic cancers. Here, an integrated theranostic nanoplatform was developed for the efficient treatment of highly metastatic tumors. Versatile functions including "And" logically controlled drug release, prolonged circulation time, tumor targeting, and anti-metastasis were integrated into doxorubicin (DOX) loaded, highly integrated mesoporous silica nanoparticles (DOX@HIMSNs) for a systemic treatment of highly metastatic triple negative breast cancer (TNBC). It was found that the good therapeutic effect of DOX@HIMSN was only partially attributed to its anticancer cytotoxicity. Most importantly, DOX@HIMSN could induce anticancer immune responses including dendritic cell (DC) maturation and antitumor cytokine release. Compared with the traditional tumor chemotherapy, the integrated theranostic nanoplatform we developed not only improved the tumor specific cytotoxicity but also stimulated antitumor immune responses during the treatment.
- Published
- 2016
21. Near‐Infrared Triggered Cascade of Antitumor Immune Responses Based on the Integrated Core–Shell Nanoparticle
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Min‐Jie Li, Xian-Zheng Zhang, Mei-Zhen Zou, Xing‐Lan Ding, Si-Xue Cheng, Qian Cheng, Fan Gao, and Wu-Yang Yu
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Biomaterials ,Core shell ,Immune system ,Near infrared light ,Materials science ,Cascade ,Near-infrared spectroscopy ,Electrochemistry ,Nanoparticle ,Nanotechnology ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials - Published
- 2020
22. Recent Advances of Cell Membrane‐Coated Nanomaterials for Biomedical Applications
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Mei-Zhen Zou, Yi-Han Ma, Wen-Long Liu, Yin-Jia Cheng, Si-Yong Qin, Xian-Zheng Zhang, and Yun-Xia Sun
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Biomaterials ,Cell membrane ,medicine.anatomical_structure ,Materials science ,Electrochemistry ,medicine ,Nanotechnology ,Condensed Matter Physics ,Electronic, Optical and Magnetic Materials ,Nanomaterials - Published
- 2020
23. Fabrication of rapid-biodegradable nano-vectors for endosomal-triggered drug delivery
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Wang Xiao, Si-yuan Peng, Mei-Zhen Zou, Jun-bin Ma, Xuan Zeng, and Cai-Xia Zhang
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Drug ,media_common.quotation_subject ,Pharmaceutical Science ,Nanoparticle ,02 engineering and technology ,021001 nanoscience & nanotechnology ,030226 pharmacology & pharmacy ,Controlled release ,Hydrophobic effect ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Monomer ,chemistry ,In vivo ,Drug delivery ,Biophysics ,Viability assay ,0210 nano-technology ,media_common - Abstract
Nanoparticles that respond to specific stimulus to achieve controlled release of anticancer drug have been vastly investigated for years. In this study, we focused on the development of low-residue organosilica nanoparticles (OSNPs), which were obtained by introducing biodegradable group into the monomer. DOX was loaded via the hydrophobic interaction between benzene and the drug. The drug release results revealed that the DOX could be fast liberated from the nanoparticles under acidic condition, indicating the high pH-response of the nanoparticles and the fine protection of anticancer drug. And the results of the cell viability showed promising efficiency in cancer therapy. Importantly, supported with the TEM figures and the degradation assessment, the nanoparticles were proved to be disintegrated into low molecular weight residues, rapidly and completely, predicting the possible few remains in vivo.
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- 2020
24. Hydrogen gas improves photothermal therapy of tumor and restrains the relapse of distant dormant tumor
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Zhenlin Zhong, Xian-Zheng Zhang, Si-Yuan Peng, Cheng Zhang, Mei-Zhen Zou, Wu-Yang Yu, Miao-Deng Liu, Di-Wei Zheng, and Chu-Xin Li
- Subjects
Ammonia borane ,Biophysics ,Biocompatible Materials ,Breast Neoplasms ,Bioengineering ,Inflammation ,02 engineering and technology ,Breast tumor ,Biomaterials ,Mice ,03 medical and health sciences ,chemistry.chemical_compound ,Ammonia ,Recurrence ,Chlorocebus aethiops ,Tumor Microenvironment ,medicine ,Animals ,Homeostasis ,Humans ,Boranes ,030304 developmental biology ,0303 health sciences ,Tumor microenvironment ,Temperature ,Mammary Neoplasms, Experimental ,Membranes, Artificial ,Phototherapy ,Photothermal therapy ,021001 nanoscience & nanotechnology ,Biocompatible material ,Nanomedicine ,Systemic toxicity ,chemistry ,Mechanics of Materials ,COS Cells ,Ceramics and Composites ,Cancer research ,Female ,Gases ,medicine.symptom ,0210 nano-technology ,Oxidation-Reduction ,Neoplasm Transplantation ,HeLa Cells ,Hydrogen - Abstract
Inflammation during photothermal therapy (PTT) of tumor usually results in adverse consequences. Here, a biomembrane camouflaged nanomedicine (mPDAB) containing polydopamine and ammonia borane was designed to enhance PTT efficacy and mitigate inflammation. Polydopamine, a biocompatible photothermal agent, can effectively convert light into heat for PTT. Ammonia borane was linked to the surface of polydopamine through the interaction of hydrogen bonding, which could destroy redox homoeostasis in tumor cells and reduce inflammation by H2 release in tumor microenvironment. Owing to the same origin of outer biomembranes, mPDAB showed excellent tumor accumulation and low systemic toxicity in a breast tumor model. Excellent PTT efficacy and inflammation reduction made the mPDAB completely eliminate the primary tumors, while also restraining the outgrowth of distant dormant tumors. The biomimetic nanomedicine shows potentials as a universal inflammation-self-alleviated platform to ameliorate inflammation-related disease treatment, including but not limited to PTT for tumor.
- Published
- 2019
25. Intra/Extracellular Lactic Acid Exhaustion for Synergistic Metabolic Therapy and Immunotherapy of Tumors
- Author
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Xian-Zheng Zhang, Chuan-Jun Liu, Fan Gao, Ying Tang, Cui Huang, Mei-Zhen Zou, and Wen-Long Liu
- Subjects
Materials science ,medicine.medical_treatment ,Intracellular Space ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Mice ,chemistry.chemical_compound ,Immune system ,Cell Line, Tumor ,Neoplasms ,Extracellular ,medicine ,Animals ,General Materials Science ,Glycolysis ,Lactic Acid ,Molecular Targeted Therapy ,Tumor microenvironment ,Mechanical Engineering ,food and beverages ,Immunotherapy ,021001 nanoscience & nanotechnology ,Combined Modality Therapy ,0104 chemical sciences ,Lactic acid ,RAW 264.7 Cells ,chemistry ,Mechanics of Materials ,Cancer research ,Extracellular Space ,0210 nano-technology ,Adenosine triphosphate ,Intracellular - Abstract
Regulating the tumor microenvironment (TME) has been a promising strategy to improve antitumor therapy. Here, a red blood cell membrane (mRBC)-camouflaged hollow MnO2 (HMnO2 ) catalytic nanosystem embedded with lactate oxidase (LOX) and a glycolysis inhibitor (denoted as PMLR) is constructed for intra/extracellular lactic acid exhaustion as well as synergistic metabolic therapy and immunotherapy of tumor. Benefiting from the long-circulation property of the mRBC, the nanosystem can gradually accumulate in a tumor site through the enhanced permeability and retention (EPR) effect. The extracellular nanosystem consumes lactic acid in the TME by catalyzing its oxidation reaction via LOX. Meanwhile, the intracellular nanosystem releases the glycolysis inhibitor to cut off the source of lactic acid, as well as achieve antitumor metabolic therapy through the blockade of the adenosine triphosphate (ATP) supply. Both the extracellular and intracellular processes can be sensitized by O2 , which can be produced during the decomposition of endogenous H2 O2 catalyzed by the PMLR nanosystem. The results show that the PMLR nanosystem can ceaselessly remove lactic acid, and then lead to an immunocompetent TME. Moreover, this TME regulation strategy can effectively improve the antitumor effect of anti-PDL1 therapy without the employment of any immune agonists to avoid the autoimmunity.
- Published
- 2019
26. A Multifunctional Biomimetic Nanoplatform for Relieving Hypoxia to Enhance Chemotherapy and Inhibit the PD-1/PD-L1 Axis
- Author
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Xian-Zheng Zhang, Jing-Jie Ye, Wen-Long Liu, Di-Wei Zheng, Jin-Yue Zeng, Mei-Zhen Zou, Chu-Xin Li, and Fan Gao
- Subjects
medicine.medical_treatment ,Programmed Cell Death 1 Receptor ,Antineoplastic Agents ,02 engineering and technology ,CD8-Positive T-Lymphocytes ,010402 general chemistry ,01 natural sciences ,B7-H1 Antigen ,Biomaterials ,Immune system ,Antigen ,Biomimetics ,PD-L1 ,Cell Line, Tumor ,Neoplasms ,medicine ,Animals ,Humans ,General Materials Science ,Doxorubicin ,Chemotherapy ,Tumor microenvironment ,biology ,Cell Death ,Chemistry ,Imidazoles ,General Chemistry ,Immunotherapy ,021001 nanoscience & nanotechnology ,Catalase ,Hypoxia-Inducible Factor 1, alpha Subunit ,0104 chemical sciences ,Mice, Inbred C57BL ,Oxygen ,Tumor progression ,biology.protein ,Cancer research ,Zeolites ,Cytokines ,Nanoparticles ,Tumor Hypoxia ,0210 nano-technology ,Biotechnology ,medicine.drug ,Signal Transduction - Abstract
Hypoxia is reported to participate in tumor progression, promote drug resistance, and immune escape within tumor microenvironment, and thus impair therapeutic effects including the chemotherapy and advanced immunotherapy. Here, a multifunctional biomimetic core-shell nanoplatform is reported for improving synergetic chemotherapy and immunotherapy. Based on the properties including good biodegradability and functionalities, the pH-sensitive zeolitic imidazolate framework 8 embedded with catalase and doxorubicin constructs the core and serves as an oxygen generator and drug reservoir. Murine melanoma cell membrane coating on the core provides tumor targeting ability and elicits an immune response due to abundance of antigens. It is demonstrated that this biomimetic core-shell nanoplatform with oxygen generation can be partial to accumulate in tumor and downregulate the expression of hypoxia-inducible factor 1α, which can further enhance the therapeutic effects of chemotherapy and reduce the expression of programmed death ligand 1 (PD-L1). Combined with immune checkpoints blockade therapy by programmed death 1 (PD-1) antibody, the dual inhibition of the PD-1/PD-L1 axis elicits significant immune response and presents a robust effect in lengthening tumor recurrent time and inhibiting tumor metastasis. Consequently, the multifunctional nanoplatform provides a potential strategy of synergetic chemotherapy and immunotherapy.
- Published
- 2018
27. A multifunctional metal–organic framework based tumor targeting drug delivery system for cancer therapy
- Author
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Shuang-Shuang Wan, Xiao-Gang Wang, Hong Cheng, Wei-Hai Chen, Mei-Zhen Zou, Xian-Zheng Zhang, Hexiang Deng, Zhiyue Dong, and Jia-Wei Huo
- Subjects
Biodistribution ,Materials science ,Biocompatibility ,Antineoplastic Agents ,Pharmacology ,Surface coating ,Drug Delivery Systems ,Targeted drug delivery ,In vivo ,COS Cells ,Chlorocebus aethiops ,Drug delivery ,Cancer cell ,Animals ,Humans ,Nanoparticles ,Surface modification ,Tissue Distribution ,General Materials Science ,HeLa Cells - Abstract
Drug delivery systems (DDSs) with biocompatibility and precise drug delivery are eagerly needed to overcome the paradox in chemotherapy that high drug doses are required to compensate for the poor biodistribution of drugs with frequent dose-related side effects. In this work, we reported a metal-organic framework (MOF) based tumor targeting DDS developed by a one-pot, and organic solvent-free "green" post-synthetic surface modification procedure, starting from the nanoscale MOF MIL-101. Owing to the multifunctional surface coating, premature drug release from this DDS was prevented. Due to the pH responsive benzoic imine bond and the redox responsive disulfide bond at the modified surface, this DDS exhibited tumor acid environment enhanced cellular uptake and intracellular reducing environment triggered drug release. In vitro and in vivo results showed that DOX loaded into this DDS exhibited effective cancer cell inhibition with much reduced side effects.
- Published
- 2015
28. Aggressive Man‐Made Red Blood Cells for Hypoxia‐Resistant Photodynamic Therapy
- Author
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Jun Feng, Ming-Kang Zhang, Wen-Long Liu, Chu-Xin Li, Zu-Yang He, Miao-Deng Liu, Tao Liu, Xian-Zheng Zhang, Mei-Zhen Zou, Wu-Yang Yu, and Zi-Hao Li
- Subjects
Erythrocytes ,Blood transfusion ,Materials science ,medicine.medical_treatment ,Mean corpuscular hemoglobin ,Photodynamic therapy ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Cell membrane ,medicine ,Animals ,General Materials Science ,Photosensitizer ,Photosensitizing Agents ,medicine.diagnostic_test ,Mechanical Engineering ,Hypoxia (medical) ,021001 nanoscience & nanotechnology ,Cell Hypoxia ,0104 chemical sciences ,Oxygen ,Red blood cell ,medicine.anatomical_structure ,Photochemotherapy ,Mechanics of Materials ,Cancer research ,Hemoglobin ,medicine.symptom ,0210 nano-technology ,circulatory and respiratory physiology - Abstract
Extreme hypoxia of tumors represents the most notable barrier against the advance of tumor treatments. Inspired by the biological nature of red blood cells (RBCs) as the primary oxygen supplier in mammals, an aggressive man-made RBC (AmmRBC) is created to combat the hypoxia-mediated resistance of tumors to photodynamic therapy (PDT). Specifically, the complex formed between hemoglobin and enzyme-mimicking polydopamine, and polydopamine-carried photosensitizer is encapsulated inside the biovesicle that is engineered from the recombined RBC membranes. The mean corpuscular hemoglobin of AmmRBCs reaches about tenfold as high as that of natural RBCs. Owing to the same origin of outer membranes, AmmRBCs share excellent biocompatibility with parent RBCs. The introduced polydopamine plays the role of the antioxidative enzymes existing inside RBCs to effectively prevent the oxygen-carrying hemoglobin from the oxidation damage during the circulation. This biomimetic engineering can accumulate in tumors, permit in situ efficient oxygen supply, and impose strong PDT efficacy toward the extremely hypoxic tumor with complete tumor elimination. The man-made pseudo-RBC shows potentials as a universal oxygen-self-supplied platform to sensitize hypoxia-limited tumor treatment means, including but not limited to PDT. Meanwhile, this study offers ideas to the production of artificial substitutes of packed RBCs for clinical blood transfusion.
- Published
- 2018
29. Highly Integrated Nano-Platform for Breaking the Barrier between Chemotherapy and Immunotherapy.
- Author
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Di-Wei Zheng, Jia-Li Chen, Jing-Yi Zhu, Lei Rong, Bin Li, Qi Lei, Jin-Xuan Fan, Mei-Zhen Zou, Cao Li, Si-Xue Cheng, Zushun Xu, and Xian-Zheng Zhang
- Published
- 2016
- Full Text
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