103 results on '"Fude Feng"'
Search Results
2. Photoluminescent Properties and Mechanism of Novel Cyanine‐Borondifluoride Curcuminoid Hybrids as Red‐to‐Near Infrared and Endoplasmic Reticulum‐Targeting Dyes
- Author
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Shan‐Shan Gong, Li‐Li Chen, Ke Du, Yuwen Yin, Rui‐Xue Yang, Rongwei Shi, Blake R. Peterson, Fude Feng, and Qi Sun
- Subjects
Organic Chemistry ,General Chemistry ,Catalysis - Published
- 2023
3. Noncovalent Engineering of Apoferritin with a PEGylated [FeFe] Hydrogenase Mimic for In Situ Polymerization
- Author
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Xiaoxuan Wei, Luyang Ji, Jinlei Peng, Ke Du, and Fude Feng
- Subjects
Biomaterials ,Hydrogenase ,Polymers ,Apoferritins ,Biochemistry (medical) ,Biomedical Engineering ,General Chemistry ,Polyethylene Glycols ,Polymerization - Abstract
Apoferritin can act as a scaffold for functionalization in the inner and outer surfaces. However, traditional covalent modification methods have a risk of disrupting the structure and physicochemical properties of apoferritin. Herein, we report a method for designing versatile apoferritin-based nanosystems through noncovalent interaction between a PEGylated [FeFe]-hydrogenase mimic (FeFe-PEG-N
- Published
- 2022
4. Visible Light and Glutathione Dually Responsive Delivery of a Polymer-Conjugated Temozolomide Intermediate for Glioblastoma Chemotherapy
- Author
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Ke Du, Qiuyu Xia, Jian Sun, and Fude Feng
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Materials science ,Light ,Cell Survival ,Polymers ,Surface Properties ,Conjugated system ,Micelle ,chemistry.chemical_compound ,Drug Delivery Systems ,Cell Line, Tumor ,Glioma ,Temozolomide ,medicine ,Humans ,Prodrugs ,General Materials Science ,Particle Size ,Triazene ,Antineoplastic Agents, Alkylating ,Cell Proliferation ,chemistry.chemical_classification ,Molecular Structure ,Glutathione ,Prodrug ,medicine.disease ,Drug Liberation ,chemistry ,Cancer research ,Thiol ,Drug Screening Assays, Antitumor ,Glioblastoma ,medicine.drug - Abstract
Temozolomide (TMZ) is a prodrug of 5-(3-methyltriazene-1-yl)imidazole-4-carboxamide (MTIC, short-lived) and used as a first-line therapy drug for glioblastoma multiforme (GBM). However, little progress has been made in regulating the kinetics of TMZ to MTIC degradation to improve the therapeutic effect, particularly in the case of TMZ-resistant GBM. In this work, we introduced a strategy to cage MTIC by N-acylation of the triazene moiety to boost the MTIC stability, designed a diblock copolymer-based MTIC prodrug installed with a disulfide linkage, and achieved self-assembled polymer micelles without the concern of MTIC leakage under physiological conditions. Polymer micelles could be induced to disassemble by stimuli factors such as glutathione (GSH) and visible light irradiation through thiol/sulfide exchange and homolytic sulfide scission mechanisms, which contributed to MTIC release in GSH-dependent and GSH-independent pathways. The in vitro results demonstrated that microenvironment-responsive polymeric micelles benefited the suppression of both TMZ-sensitive and TMZ-resistant GBM cells. The chemistry of polymer-MTIC prodrug provided a new option for TMZ-based glioma treatment.
- Published
- 2021
5. Photocatalytic Generation of Hydrogen Radical (H•) with GSH for Photodynamic Therapy
- Author
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Jinlei Peng, Ke Du, Jian Sun, Xianli Yang, Xia Wang, Xiaoran Zhang, Gang Song, and Fude Feng
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General Medicine ,General Chemistry ,Catalysis - Abstract
As a reactive hydrogen species, hydrogen radical (H•) scarcely sees applications in tumor biological therapy due to very limited resources of bio-friendly H• generator. In this work, we report that TAF can act as an organic photosensitizer as well as an efficient photocatalytic H• generator with reduced glutathione (GSH) as a fuel. The intracellular H•-participating photocatalysis leads to cell death by triple amplification of oxidative stress via a hybrid ferroptosis-apoptosis pathway under normoxia or apoptosis through biological reductions under hypoxia. TAF presents ultrahigh photocytotoxicity and excellent biosafety with photocytotoxicity index at an order of magnitude of 102-103 on both normoxic and hypoxic cells. The in vitro data suggest that H• therapy is promising to overcome the challenge of tumor hypoxia at low doses of both photocatalyst and light. And the capability of near infrared two-photon excitation would benefit broad biological applications.
- Published
- 2022
6. Dithiol‐Activated Bioorthogonal Chemistry for Endoplasmic Reticulum‐Targeted Synergistic Chemophototherapy
- Author
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Jian Sun, Xiaoran Zhang, Xia Wang, Jinlei Peng, Gang Song, Yufei Di, Fude Feng, and Shu Wang
- Subjects
General Chemistry ,General Medicine ,Catalysis - Abstract
The controlled intracellular release of nitrite is still an unmet challenge due to the lack of bio-friendly donors, and the antitumor effect of nitrite is limited by its physiologically inert activity. Herein, we designed benzothiadiazole-based organic nitrite donors that are stable against bio-relevant species but selectively respond to dithiol species through S
- Published
- 2022
7. Photoactivated In Situ Generation of Near Infrared Cyanines for Spatiotemporally Controlled Fluorescence Imaging in Living Cells
- Author
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Gang Song, Hao Heng, Jiaqi Wang, Ronghua Liu, Yiming Huang, Huan Lu, Ke Du, Fude Feng, and Shu Wang
- Subjects
In situ ,Indole test ,Fluorescence-lifetime imaging microscopy ,Near-Infrared Fluorescence Imaging ,Fluorophore ,Molecular Structure ,010405 organic chemistry ,Optical Imaging ,General Medicine ,General Chemistry ,Carbocyanines ,Photochemical Processes ,010402 general chemistry ,Photochemistry ,01 natural sciences ,Catalysis ,0104 chemical sciences ,chemistry.chemical_compound ,Monomer ,chemistry ,Humans ,Photosensitizer ,Irradiation ,Fluorescent Dyes ,HeLa Cells - Abstract
Photoactivated trimerization of 2,3,3-trimethyl-3 H -indole derivatives created near infrared fluorophore Cy5. The synthetic method is air-tolerant, photosensitizer free, metal free, and condensation agent free. Living cells make Cy5 on a time scale of minutes under white light irradiation at a low power intensity, with the monomer as the only exogenous agent. The new method is promising to find applications in cell studies for in situ spatiotemporally controlled fluorescence imaging in living cells with low background signal.
- Published
- 2021
8. Intrinsic Mitochondrial Reactive Oxygen Species (ROS) Activate the In Situ Synthesis of Trimethine Cyanines in Cancer Cells
- Author
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Hao Heng, Gang Song, Xuetong Cai, Jian Sun, Ke Du, Xiaoran Zhang, Xia Wang, Fude Feng, and Shu Wang
- Subjects
Neoplasms ,Quinolines ,General Chemistry ,General Medicine ,Carbocyanines ,Reactive Oxygen Species ,Catalysis ,Fluorescent Dyes ,Mitochondria - Abstract
Environment-responsive in situ synthesis of molecular fluorescent dyes is challenging. Herein, we develop a photoextension strategy to make trimethine cyanines with decent conversion efficiency (up to 81 %) using 1-butyl 2,3,3-trimethyl 3H-indole derivatives as the sole precursors, and demonstrate a free radical mechanism. In the inducer-extension stage, free radicals and reactive oxygen species (ROS) were able to mediate similar reactions with no assistance of light. We explored a Mito-extension strategy to in situ synthesize trimethine cyanines in the living cells. The cellular ROS-dependence provided a foundation for preferential cyanine expression in cancer cells. Finally, we applied an iodized precursor as an intrinsic ROS-activated theranostic agent that integrated mitochondria-targeted cyanine synthesis, cell imaging and phototherapy.
- Published
- 2022
9. Temozolomide–Doxorubicin Conjugate as a Double Intercalating Agent and Delivery by Apoferritin for Glioblastoma Chemotherapy
- Author
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Hao Heng, Fude Feng, Ke Du, and Qiuyu Xia
- Subjects
Materials science ,Cell Survival ,Surface Properties ,Molecular Conformation ,Antineoplastic Agents ,Conjugated system ,01 natural sciences ,03 medical and health sciences ,chemistry.chemical_compound ,Drug Delivery Systems ,0302 clinical medicine ,Cell Line, Tumor ,Temozolomide ,medicine ,Humans ,Moiety ,General Materials Science ,Doxorubicin ,Particle Size ,Cell Proliferation ,Drug Carriers ,Brain Neoplasms ,Viscosity ,010401 analytical chemistry ,Prodrug ,0104 chemical sciences ,chemistry ,030220 oncology & carcinogenesis ,Apoferritins ,Biophysics ,Drug Screening Assays, Antitumor ,Nanocarriers ,Glioblastoma ,DNA ,medicine.drug ,Conjugate - Abstract
We designed a conjugated compound by coupling temozolomide (TMZ) with doxorubicin (DOX) via an acylhydrazone linkage as a potential prodrug used for glioblastoma multiforme (GBM) treatment. Viscosity and spectroscopic studies revealed that the drug conjugate could act as a nonclassical double intercalating agent. Although free TMZ is an inefficient DNA binder in comparison to DOX, the TMZ moiety interacted with DNA as an induced intercalator, arising from the synergistic effect of DOX moiety that mediated conformational changes of the DNA helix. Two binding modes were proposed to interpret the double intercalating effect of the drug conjugate on intra- and inter-DNA interactions that could cause DNA cross-linking and fibril aggregates. We also developed a delivery nanoplatform with a loading efficiency of 83% using copper-bound apoferritin as a nanocarrier. In sharp contrast to the short half-life of free TMZ, the nanocomposite was stable under physiological conditions without detectable drug decomposition after a 2 week storage, and drug release was activatable in the presence of glutathione at millimolar levels. The antitumor effect of the drug conjugate and nanocomposite against GBM cells was reported to demonstrate the potential therapeutic applications of double intercalating materials.
- Published
- 2020
10. GSH and H 2 O 2 Co‐Activatable Mitochondria‐Targeted Photodynamic Therapy under Normoxia and Hypoxia
- Author
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Jiajie Diao, Fude Feng, Shu Wang, Jian Sun, Xuetong Cai, and Ke Du
- Subjects
chemistry.chemical_classification ,Reactive oxygen species ,010405 organic chemistry ,Chemistry ,Superoxide ,Singlet oxygen ,medicine.medical_treatment ,Photodynamic therapy ,General Medicine ,General Chemistry ,Glutathione ,010402 general chemistry ,01 natural sciences ,Redox ,Catalysis ,0104 chemical sciences ,chemistry.chemical_compound ,medicine ,Biophysics ,Photosensitizer ,Phototoxicity - Abstract
Currently, photosensitizers (PSs) that are microenvironment responsive and hypoxia active are scarcely available and urgently desired for antitumor photodynamic therapy (PDT). Presented herein is the design of a redox stimuli activatable metal-free photosensitizer (aPS), also functioning as a pre-photosensitizer as it is converted to a PS by the mutual presence of glutathione (GSH) and hydrogen peroxide (H2 O2 ) with high specificity on a basis of domino reactions on the benzothiadiazole ring. Superior to traditional PSs, the activated aPS contributed to efficient generation of reactive oxygen species including singlet oxygen and superoxide ion through both type 1 and type 2 pathways, alleviating the aerobic requirement for PDT. Equipped with a triphenylphosphine ligand for mitochondria targeting, mito aPS showed excellent phototoxicity to tumor cells with low light fluence under both normoxic and hypoxic conditions, after activation by intracellular GSH and H2 O2 . The mito aPS was also compatible to near infrared PDT with two photon excitation (800 nm) for extensive bioapplications.
- Published
- 2020
11. Precise engineering of apoferritin through site-specific host–guest binding
- Author
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Hao Heng, Fude Feng, Weijian Chen, Ke Du, Xiao Li, Xiaoxuan Wei, Xuewei Wang, and Shu Wang
- Subjects
Models, Molecular ,Azides ,Porphyrins ,Surface engineering ,Protein Engineering ,Catalysis ,Polyethylene Glycols ,chemistry.chemical_compound ,Materials Chemistry ,Moiety ,Binding site ,Bifunctional ,Binding Sites ,Molecular Structure ,Metals and Alloys ,General Chemistry ,Protein engineering ,Combinatorial chemistry ,Porphyrin ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,chemistry ,Apoferritins ,Ceramics and Composites ,Ethylene glycol ,Macromolecule - Abstract
The surface engineering of the apoferritin shell by means of traditional chemical modifications usually suffers from site inaccuracy and insufficient conjugation. This report describes a non-covalent method for precise modulation of the apoferritin surface without alteration of amino acid residues. A bifunctional macromolecule, structured as azide-poly(ethylene glycol)-porphyrin (termed TPA), was synthesized. TPA was observed to be able to recognize and bind apoferritin in a 12 : 1 stoichiometry with a higher binding affinity than arachidonate, thanks to the specific host-guest interaction between the pocket of each two-fold channel and the porphyrin moiety. This method allows for site-specific engineering of the apoferritin surface with on demand functionalities and optimization of drug encapsulation.
- Published
- 2020
12. Self-Assembly of Albumin and [FeFe]-Hydrogenase Mimics for Photocatalytic Hydrogen Evolution
- Author
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Fude Feng, Shuyi Li, Xiantao Hu, and Weijian Chen
- Subjects
Hydrogenase ,biology ,Chemistry ,Biochemistry (medical) ,Biomedical Engineering ,Active site ,General Chemistry ,respiratory system ,Combinatorial chemistry ,Catalysis ,Turnover number ,Biomaterials ,Ovalbumin ,biology.protein ,Photocatalysis ,Self-assembly ,Nanogel - Abstract
To imitate the protein microenvironment surrounding the Fe2S2 active site, we established a convenient method to prepare self-assembled ovalbumin (OVA) nanogels that noncovalently incorporated hydr...
- Published
- 2022
13. Apoferritin as a Carrier of Cu(II) Diethyldithiocarbamate and Biomedical Application for Glutathione-Responsive Combination Chemotherapy
- Author
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Fude Feng, Ke Du, Xiao Li, and Jian Sun
- Subjects
Chemistry ,Biochemistry (medical) ,Biomedical Engineering ,chemistry.chemical_element ,Combination chemotherapy ,General Chemistry ,Glutathione ,Pharmacology ,Copper ,Biomaterials ,chemistry.chemical_compound ,Disulfiram ,medicine ,Antialcoholic drug ,medicine.drug - Abstract
The antialcoholic drug disulfiram (DSF) has attractive biomedical interests for its anticancer effects, particularly in combination use with copper. CuET, the complex of copper and diethyldithiocarbamate (DTC) that is derived from disulfiram, exhibits high redox stability against glutathione, cysteine, and hydrogen peroxide, but appears reactive to ascorbic acid and superoxide ions. By virtue of the copper binding property, apoferritin is developed as a carrier of CuET to alleviate concerns of poor water solubility and nonspecific toxicity and shows superior stability and protection over human serum albumin in nanocomposite manipulation. CuET and doxorubicin are codelivered by apoferritin with excellent efficiency (97%). The glutathione-responsive system demonstrates enhanced antitumor effect and potentials for combination tumor therapy.
- Published
- 2022
14. Biomimetic Polymer-Templated Copper Nanoparticles Stabilize a Temozolomide Intermediate for Chemotherapy against Glioblastoma Multiforme
- Author
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Ke Du, Xia Wang, Fude Feng, and Andi Hu
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Chemotherapy ,Temozolomide ,Chemistry ,Polymers ,medicine.medical_treatment ,Biochemistry (medical) ,Biomedical Engineering ,Nanoparticle ,General Chemistry ,medicine.disease ,nervous system diseases ,Biomaterials ,Metabolic kinetics ,Biomimetics ,medicine ,Cancer research ,Humans ,Nanoparticles ,Glioblastoma ,Copper ,medicine.drug - Abstract
The short half-life of temozolomide (TMZ) limits its therapeutic effect on highly aggressive glioblastoma (GBM). Few approaches attempting to intervene the metabolic kinetics of TMZ are successful. Herein, we designed anionic copolymers via radical polymerization to prepare polymer-coated small copper nanoclusters, taking advantage of the role of pendent thymine groups as a template. The active and key intermediate of TMZ, typically called 3-methyl-(triazen-1-yl)imidazole-4-carboxamide (MTIC), was stabilized by copper under physiological (slightly alkaline) conditions, alleviating concerns associated with spontaneous drug degradation and nonspecific drug activation. Importantly, the complexes formed by MTIC and copper nanoclusters could catalyze the Fenton reaction to generate hydroxyl radicals and also respond to pH and glutathione to release therapeutic MTIC, which allows combined chemotherapy and chemodynamic therapy against GBM cells and paves a way for circumventing the complication of TMZ resistance.
- Published
- 2022
15. Inhibition of AIM2 inflammasome-mediated pyroptosis by Andrographolide contributes to amelioration of radiation-induced lung inflammation and fibrosis
- Author
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Jian Sun, Lihong Shen, Guoliang Deng, Jian Gao, Wenjie Guo, Xinchen Sun, Xinni Shan, Qiang Xu, Shuang Peng, Chunhong Jiang, Xiaoling Yang, Yanhong Gu, Zhigang Chang, Yang Sun, Fude Feng, and Ling-Dong Kong
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Cancer Research ,Inflammasomes ,Andrographolide ,medicine.medical_treatment ,Immunology ,Radiation-Protective Agents ,Inflammation ,Lung injury ,Pharmacology ,Abnormalities, Radiation-Induced ,Article ,Mice ,Cellular and Molecular Neuroscience ,chemistry.chemical_compound ,AIM2 ,Fibrosis ,Pyroptosis ,medicine ,Animals ,Humans ,lcsh:QH573-671 ,Lung ,Respiratory tract diseases ,business.industry ,lcsh:Cytology ,Inflammasome ,Lung Injury ,Cell Biology ,medicine.disease ,DNA-Binding Proteins ,Disease Models, Animal ,Cytokine ,chemistry ,Diterpenes ,medicine.symptom ,business ,medicine.drug - Abstract
Radiation-induced lung injury (RILI) is one of the most common and fatal complications of thoracic radiotherapy, whereas no effective interventions are available. Andrographolide, an active component extracted from Andrographis paniculate, is prescribed as a treatment for upper respiratory tract infection. Here we report the potential radioprotective effect and mechanism of Andrographolide on RILI. C57BL/6 mice were exposed to 18 Gy of whole thorax irradiation, followed by intraperitoneal injection of Andrographolide every other day for 4 weeks. Andrographolide significantly ameliorated radiation-induced lung tissue damage, inflammatory cell infiltration, and pro-inflammatory cytokine release in the early phase and progressive fibrosis in the late phase. Moreover, Andrographolide markedly hampered radiation-induced activation of the AIM2 inflammasome and pyroptosis in vivo. Furthermore, bone marrow-derived macrophages (BMDMs) were exposed to 8 Gy of X-ray radiation in vitro and Andrographolide significantly inhibited AIM2 inflammasome mediated-pyroptosis in BMDMs. Mechanistically, Andrographolide effectively prevented AIM2 from translocating into the nucleus to sense DNA damage induced by radiation or chemotherapeutic agents in BMDMs. Taken together, Andrographolide ameliorates RILI by suppressing AIM2 inflammasome mediated-pyroptosis in macrophage, identifying Andrographolide as a novel potential protective agent for RILI.
- Published
- 2019
16. One pot synthesis and self-assembly of methylene blue-backboned polymers
- Author
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Fude Feng, Xuetong Cai, Rong Wang, Luyang Ji, and Hao Tang
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chemistry.chemical_classification ,Dissipative particle dynamics ,Metals and Alloys ,General Chemistry ,Polymer ,Photochemistry ,Catalysis ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,Monomer ,chemistry ,Polymerization ,Materials Chemistry ,Ceramics and Composites ,Self-assembly ,Methylene ,Alkyl ,Methylene blue - Abstract
Studies of methylene blue-backboned polymers (MBPs) are hindered by the limited availability of polymerization methods. Herein, we developed an oxidative polymerization method to produce MBPs. The polymerization is performed in aqueous medium, and is organic solvent-free, heavy metal-free, time-efficient (on a timescale of minutes), and does not need pre-formed methylene blue chromophores. The effects of the alkyl chains of the MBPs on the photophysical properties and self-assembly behavior (e.g., vesicles and nanorings) are significant, which highlights the possibility of controlling the MBP properties via rationally tailoring the functionality of the MBP monomers prior to polymerization. Importantly, the self-assembly structures can be predicted using the dissipative particle dynamics (DPD) simulation method.
- Published
- 2021
17. 聚合物中螺吡喃原位“自笼”及光响应调控
- Author
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He Hang, Fude Feng, Xuetong Cai, Liheng Feng, Gang Song, Jiaqi Wang, Zhihong Wei, and Yuxi Tian
- Subjects
General Chemical Engineering ,Materials Chemistry ,General Chemistry ,Biochemistry - Published
- 2021
18. A photosynthesis-inspired supramolecular system: caging photosensitizer and photocatalyst in apoferritin
- Author
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Xuewei Wang, Xiao Li, Yajing Gao, Fude Feng, Luyang Ji, Xiaoran Zhang, Weijian Chen, and Xuetong Cai
- Subjects
0106 biological sciences ,0301 basic medicine ,Luminescence ,Supramolecular chemistry ,chemistry.chemical_element ,Plant Science ,Photochemistry ,01 natural sciences ,Biochemistry ,Catalysis ,Ruthenium ,Nanocomposites ,Artificial photosynthesis ,Supramolecular assembly ,Electron Transport ,03 medical and health sciences ,Electron transfer ,Non-covalent interactions ,Photosensitizer ,Photosynthesis ,chemistry.chemical_classification ,Photosensitizing Agents ,Cell Biology ,General Medicine ,Hydrogen-Ion Concentration ,030104 developmental biology ,chemistry ,Apoferritins ,Spectrophotometry, Ultraviolet ,Hydrogen ,010606 plant biology & botany - Abstract
Inspired by the bioinorganic structure of natural [FeFe]-hydrogenase ([FeFe]-H2ase) that possesses iron sulfur clusters to catalyze proton reduction to hydrogen (H2), we design a supramolecular photosystem by sequentially integrating hydrophobic ruthenium complex (as a photosensitizer) and diiron dithiolate complex (as a photocatalyst) into the inner surface or cavity of apoferritin via noncovalent interactions. This platform allows photosensitizer and catalyst to localize in a close proximity and short-distance electron transfer process to occur within a confined space. The resulted uniform core-shell nanocomposites were stable and well dispersed in water, and showed enhanced H2 generation activity in acidic solution as compared to the homogenous system without apoferritin participation.
- Published
- 2019
19. Mitochondria Targeting Fluorescent Probes Based on through Bond‐Energy Transfer for Mutually Imaging Signaling Molecules H 2 S and H 2 O 2
- Author
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Xiao Li, Tiantian Wu, Jia Cao, Fude Feng, Xuewei Wang, Qi Sun, Yajie Zhang, Jian Sun, and Xiantao Hu
- Subjects
Cell signaling ,010405 organic chemistry ,Chemistry ,Organic Chemistry ,General Chemistry ,Mitochondrion ,010402 general chemistry ,01 natural sciences ,Fluorescence ,Catalysis ,0104 chemical sciences ,Rhodamine ,Crosstalk (biology) ,chemistry.chemical_compound ,Biophysics ,Moiety ,Bond energy ,Fluorescence response - Abstract
Reactive signaling molecules participate in varieties of biochemical reactions, and methods to detect their mutual existence and crosstalk are in urgent demand. A benzothiadiazole-based handle was designed to fluorescently respond to the co-existence of H2 S and H2 O2 under pseudo-physiological conditions on a basis of a thiyl-radical-mediated mechanism that accounts for the rapid and efficient domino-like reaction processes. Then the handle motif was attached to a rhodamine moiety by means of an ethynylene linkage, and achieved a significant H2 S-H2 O2 mutual response in the mitochondria of living cells. Theoretical calculations supported that a through bond energy transfer mechanism contributes to the drastic fluorescence response.
- Published
- 2019
20. Diiron Dithiolate Complex Induced Helical Structure of Histone and Application in Photochemical Hydrogen Generation
- Author
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Fude Feng, Ke Du, Qiuyu Xia, Shuyi Li, Jian Sun, Weijian Chen, and Xiantao Hu
- Subjects
0106 biological sciences ,0301 basic medicine ,Diiron dithiolate ,Materials science ,biology ,Photochemistry ,Protein Conformation ,Ferric Compounds ,01 natural sciences ,Catalysis ,Histones ,03 medical and health sciences ,030104 developmental biology ,Histone ,biology.protein ,General Materials Science ,Sulfhydryl Compounds ,Hydrogen ,010606 plant biology & botany ,Hydrogen production - Abstract
Very-lysine-rich calf thymus histone proteins form disordered structure and hydrophobic interaction-driven aggregates in weakly acidic solution. We reported that the conjugation of diiron dithiolate complex to the lysine residues induced formation of helical conformation and condensed nanoassemblies with a high loading capacity up to 18.7 wt %. The incorporated diiron dithiolate complex showed photocatalytic activity for hydrogen evolution in aqueous solutions, with a turnover number (based on [FeFe] catalyst moiety) up to 359 that was more than 6 times that of the free catalyst. The increase of helical conformation in proteins was well correlated to the increasing enhancement of photocatalytic activity. We demonstrated that the [FeFe]-hydrogenase-mimic biohybrid system based on the photocatalyst-induced protein conformational conversion and reassembly is efficient for hydrogen generation regardless of the relatively large size.
- Published
- 2019
21. Light-Controlled in Vitro Gene Delivery Using Polymer-Tethered Spiropyran as a Photoswitchable Photosensitizer
- Author
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Tiantian Wu, Fude Feng, Jinkai Ji, and Yajie Zhang
- Subjects
Indoles ,Materials science ,Light ,Cell Survival ,02 engineering and technology ,Gene delivery ,Transfection ,010402 general chemistry ,01 natural sciences ,chemistry.chemical_compound ,Photochromism ,Fluorescence Resonance Energy Transfer ,Humans ,Polyethyleneimine ,Benzopyrans ,General Materials Science ,Photosensitizer ,Spiropyran ,Polyethylenimine ,Photosensitizing Agents ,Rhodamines ,Singlet oxygen ,Cationic polymerization ,DNA ,Nitro Compounds ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Microscopy, Fluorescence ,chemistry ,Biophysics ,Nanoparticles ,DNA supercoil ,Fluorescein ,Reactive Oxygen Species ,0210 nano-technology ,HeLa Cells - Abstract
A gene delivery system using spiropyran as a photoswitchable photosensitizer for the controlled photochemical internalization effect was developed by engineering the outer coating of a polyethylenimine/DNA complex with a small amount of spiropyran-containing cationic copolymers. The successful binding of cationic polymers by the polyethylenimine coating was detected by the distance-sensitive fluorescence resonance energy-transfer technique that evidenced the occurrence of energy transfer between fluorescein-labeled cationic copolymers and polyethylenimine-condensed rhodamine-labeled DNA. The ternary polyplexes feature reversible controllability of singlet oxygen generation based on the dual effect of spiropyrans in photochromism and aggregation-induced enhanced photosensitization, allowing significant light-induced amplification of bPEI-mediated in vitro transgene efficiency (from original 15% to final 91%) at a low DNA dose, with the integrity of supercoiled DNA structure unaffected. The use of spiropyran without the need of other photosensitizers circumvents the issue of uncontrolled long-lasting photocytotoxicity in gene delivery.
- Published
- 2019
22. Iron sulfur clusters in protein nanocages for photocatalytic hydrogen generation in acidic aqueous solutions
- Author
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Weijian Chen, Fan Zhu, Xiantao Hu, Fude Feng, Shuyi Li, Chi Zhang, Xiao Li, and Guosong Shen
- Subjects
Aqueous solution ,010405 organic chemistry ,Chemistry ,Supramolecular chemistry ,chemistry.chemical_element ,General Chemistry ,010402 general chemistry ,Photochemistry ,01 natural sciences ,Sulfur ,0104 chemical sciences ,Catalysis ,Nanocages ,Photocatalysis ,Moiety ,Hydrogen production - Abstract
We took advantage of the iron binding affinity of apoferritin to immobilize iron–sulfur clusters into apoferritin up to 312 moieties per protein, with a loading rate as high as 25 wt%. The photocatalytic hydrogen generation activity in acidic aqueous solutions was achieved with TONs up to 31 (based on a single catalyst moiety) or 8.3 × 103 (based on a single protein) upon 3 h of visible light irradiation. The present study provides a versatile strategy to construct uniform protein/photocatalyst supramolecular systems with FeFe-H2ase activity.
- Published
- 2019
23. Synthesis of a sulfonated methylene blue-backboned polymer for biodetections
- Author
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Xuetong Cai, Andi Hu, and Fude Feng
- Subjects
Process Chemistry and Technology ,General Chemical Engineering - Published
- 2022
24. Aggregation-Enhanced Two-Photon Absorption of Anionic Conjugated Polyelectrolytes
- Author
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Pradeepkumar Jagadesan, Kirk S. Schanze, Fude Feng, and Silvano R. Valandro
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Aqueous solution ,Chemistry ,Absorption cross section ,02 engineering and technology ,Conjugated system ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Photochemistry ,01 natural sciences ,Conjugated Polyelectrolytes ,Two-photon absorption ,Polyelectrolyte ,0104 chemical sciences ,Phenylene ,General Materials Science ,Physical and Theoretical Chemistry ,Absorption (chemistry) ,0210 nano-technology - Abstract
The two-photon absorption properties of anionic poly(phenylene ethynylene)-type conjugated oligo- and polyelectrolytes are studied in molecularly dissolved and aggregated forms in aqueous solution. Several different polyvalent cations are used to induce aggregation. It is found that both materials in the aggregated form exhibit enhanced two-photon excited fluorescence (2PEF) and two-photon cross section (σ2) compared with the molecularly dissolved structures. The 2PEF and σ2 are unaffected by the nature of the polyvalent cation that is used to induce aggregation. The two-photon absorption cross section enhancement arises because of the increase in the difference dipole moment (Δμ) in the aggregates of the conjugated materials, an effect that is attributed to the introduction of charge transfer character into the aggregate excited state.
- Published
- 2020
25. Folate-Modified Photoelectric Responsive Polymer Microarray as Bionic Artificial Retina to Restore Visual Function
- Author
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Zheng-Hang Yu, Huai-Jin Guan, Fude Feng, Jia-Hao Liu, Mei Dong, Qun-Dong Shen, Xi Liu, Weijian Chen, Qiuyu Xia, Cheng Sun, and Yi-Nuo Yang
- Subjects
Bionics ,Nerve stimulation ,Retina ,Materials science ,genetic structures ,Polymers ,Prostheses and Implants ,Thiophenes ,Responsive polymer ,eye diseases ,Rats ,medicine.anatomical_structure ,Folic Acid ,Folic acid ,Visual function ,medicine ,Optic nerve ,Animals ,General Materials Science ,sense organs ,Neuron ,Biomedical engineering ,Visual phototransduction - Abstract
A high-optical-resolution artificial retina system that accurately communicates with the optic nerve is the main challenge in the modern biological science and bionic field. Here, we developed a bionic artificial retina possessing phototransduction "cells" with measurements even smaller than that of the neural cells. Using the technique of micrometer processing, we constructed a pyramid-shape periodic microarray of a photoreceptor. Each "sensing cell" took advantage of polythiophene derivative/fullerene derivative (PCBM) as a photoelectric converter. Because folic acid played an essential role in eye growth, we particularly modified the polythiophene derivatives with folic acid tags. Therefore, the artificial retina could enlarge the contact area and even recognize the nerve cells to improve the consequence of nerve stimulation. We implanted the artificial retina into blinded rats' eyes. Electrophysiological analysis revealed its recovery of photosensitive function 3 months after surgery. Our work provides an innovative idea for fabricating a high-resolution bionic artificial retina system. It shows great potential in artificial intelligence and biomedicine.
- Published
- 2020
26. GSH and H
- Author
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Jian, Sun, Ke, Du, Jiajie, Diao, Xuetong, Cai, Fude, Feng, and Shu, Wang
- Subjects
Photosensitizing Agents ,Photochemotherapy ,Singlet Oxygen ,Phosphines ,Cell Line, Tumor ,Drug Design ,Neoplasms ,Animals ,Humans ,Hydrogen Peroxide ,Hypoxia ,Glutathione ,Mitochondria - Abstract
Currently, photosensitizers (PSs) that are microenvironment responsive and hypoxia active are scarcely available and urgently desired for antitumor photodynamic therapy (PDT). Presented herein is the design of a redox stimuli activatable metal-free photosensitizer (aPS), also functioning as a pre-photosensitizer as it is converted to a PS by the mutual presence of glutathione (GSH) and hydrogen peroxide (H
- Published
- 2020
27. Facile crosslinking of polythiophenes by polyethylenimine via ester aminolysis for selective Cu(II) detection in water
- Author
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Yajie Zhang, Chi Zhang, Jian Sun, Fude Feng, Leilei Cao, Jinkai Ji, and Xuewei Wang
- Subjects
Polymers ,Metal ions in aqueous solution ,Biomedical Engineering ,Biophysics ,Biosensing Techniques ,Thiophenes ,02 engineering and technology ,Conjugated system ,010402 general chemistry ,01 natural sciences ,chemistry.chemical_compound ,Aminolysis ,Electrochemistry ,chemistry.chemical_classification ,Polyethylenimine ,Aqueous solution ,technology, industry, and agriculture ,Cationic polymerization ,Water ,Esters ,General Medicine ,Polymer ,021001 nanoscience & nanotechnology ,Combinatorial chemistry ,Nanostructures ,0104 chemical sciences ,Cross-Linking Reagents ,chemistry ,Polythiophene ,0210 nano-technology ,Copper ,Biotechnology - Abstract
Functionalization of π-conjugated polymers is dispensable for solubilization of the rigid and hydrophobic backbones in water. However, polymer aggregation is always present and leads to issues with complication and reproducibility in spectral properties. Herein, we reported a simple and robust method to make a series of conjugated polymer nanostructures by a crosslinking strategy. In favor of multivalency effect, polythionenes with various chain lengths were functionalized with branched polyethylenimine (PEI) via ester aminolysis reaction under mild conditions. Photophysical studies revealed the conjugated backbones could be well stabilized and dispersed in water. By taking advantage of intermolecular recognition interaction between copper ions and cationic PEI, we applied crosslinked polythiophenes as a nano probe at very low concentration (0.01 mg/mL) to fluorescently detect copper ions with high sensitivity up to 10 nM and selectivity over other metal ions in aqueous solutions, without occurrence of detectable aggregates. The overall performance of our nano probes outperforms reported water-soluble polymers-based probes, particularly in probe availability and manipulation as well as selective copper detection capability.
- Published
- 2018
28. An S-alkyl thiocarbamate-based biosensor for highly sensitive and selective detection of hypochlorous acid
- Author
-
Fude Feng and Jian Sun
- Subjects
chemistry.chemical_classification ,Hypochlorous acid ,010405 organic chemistry ,Chemistry ,High selectivity ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Fluorescence ,Combinatorial chemistry ,0104 chemical sciences ,Analytical Chemistry ,Highly sensitive ,Thiocarbamate ,chemistry.chemical_compound ,Electrochemistry ,Environmental Chemistry ,Biosensor ,Linker ,Spectroscopy ,Alkyl - Abstract
We reported a near-infrared biosensor that features a dihydromethylene blue and an S-alkyl thiocarbamate linker to detect hypochlorous acid in a drastic fluorescence turn-on response. We achieved high sensitivity at the nanomolar level and high selectivity that resolves the interference issue with mercury ions and other transition metal ions. We demonstrated the response mechanism by analysing the released segments, and investigated the imaging capability to detect both exogenous and endogenous hypochlorous acid in living cells.
- Published
- 2018
29. Spiropyran in nanoassemblies as a photosensitizer for photoswitchable ROS generation in living cells
- Author
-
Xiao Li, Tiantian Wu, Fude Feng, and Jinkai Ji
- Subjects
Spiropyran ,Chemistry ,Singlet oxygen ,medicine.medical_treatment ,Quantum yield ,Nanoparticle ,Photodynamic therapy ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Photochemistry ,01 natural sciences ,0104 chemical sciences ,chemistry.chemical_compound ,Photochromism ,Cell killing ,medicine ,Photosensitizer ,0210 nano-technology - Abstract
Reversibly controlled generation of singlet oxygen from photosensitizing nanosystems has the benefits of selective cell killing and controllable effect time, but is a challenging option for photodynamic therapies. We report a strategy for integrating photochromic spiropyrans into biocompatible cationic polymers, which involved assembling nucleic acids into functional nanoparticles without introducing additional photosensitizers and imaging agents. We found that spiropyran-containing nanoparticles have photoswitching properties for both fluorescence (with a quantum yield of up to 0.27) and singlet oxygen generation (with a quantum yield of up to 0.22) in aqueous solutions and cells, and demonstrated that spiropyrans in nanoassemblies featuring aggregation-induced enhanced photosensitization and emission could be potentially applied in photodynamic therapy studies on tumor cells.
- Published
- 2018
30. Direct visualization of enzymatic cleavage and oxidative damage by hydroxyl radicals of single-stranded DNA with a cationic polythiophene derivative
- Author
-
Yanli Tang, Fude Feng, Fang He, Shu Wang, Yuliang Li, and Daoben Zhu
- Subjects
DNA -- Research ,Thiophene -- Chemical properties ,Drugs -- Product/Service Evaluations ,Drugs -- Methods ,Chemistry - Abstract
A new method is developed for the label-free, convenient and real-time monitoring of the cleavage of single-stranded DNA by single-strand-specific S1 nuclease and hydroxyl radical based on cationic water-soluble poly[3-(3'-N,N,N-triethylamino-1'-propyloxy)-4-methyl-2,5-thiophene hydrochloride] (PMNT). Direct visualization of the DNA cleavage by the 'naked eye' is one of the important features of the assay, which provides a promising application in drug screening based on the inhibition of oxidative damage of DNA.
- Published
- 2006
31. H2S Activated Drug Release from Protein Cages
- Author
-
Jian Sun, H. S. Chen, Fude Feng, Yajie Zhang, Xiao Li, and Weijian Chen
- Subjects
chemistry.chemical_classification ,Materials science ,Nanocomposite ,Sulfide ,Hydrogen sulfide ,chemistry.chemical_element ,02 engineering and technology ,equipment and supplies ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Controlled release ,Chemical reaction ,Copper ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Biochemistry ,Drug release ,medicine ,Biophysics ,General Materials Science ,Doxorubicin ,0210 nano-technology ,medicine.drug - Abstract
We took advantage of gasotransmitter H2S as a chemical reaction-based trigger for controlled release of doxorubicin which is precoordinated by copper ions and enclosed in horse spleen apoferritin. The nanocomposite is stable at physiological pH and temperature before H2S activation. The drug release process avoids disassembly of protein shells and is controllable by the strong affinity of sulfide with copper ions. The in vitro cytotoxicity assay indicates the antitumor effect of doxorubicin toward tumor cells could be achievable by H2S activation.
- Published
- 2017
32. Protein Nanocage-Based Photo-Controlled Nitric Oxide Releasing Platform
- Author
-
Jian Sun, Weijian Chen, Yang Sun, Fude Feng, Fenli Shao, Xiao Li, Xuewei Wang, Yuyu Zhu, and Yajie Zhang
- Subjects
Materials science ,Inorganic chemistry ,High loading ,Iron–sulfur cluster ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,0104 chemical sciences ,Nitric oxide ,chemistry.chemical_compound ,Nanocages ,chemistry ,Biophysics ,General Materials Science ,0210 nano-technology ,Cytotoxicity - Abstract
A photoactive NO releasing system was constructed by incorporation of NO-bound Fe-S clusters into horse spleen apoferritin cavities with high loading efficacy. The composites retained intact core-shell structure and indicated advantages such as enhanced stability, reduced cytotoxicity, efficient cellular uptake, and photocontrolled NO releasing property.
- Published
- 2017
33. Biomimetic Polymer-Templated Copper Nanoparticles Stabilize a Temozolomide Intermediate for Chemotherapy against Glioblastoma Multiforme.
- Author
-
Xia Wang, Andi Hu, Ke Du, and Fude Feng
- Published
- 2021
- Full Text
- View/download PDF
34. Enhanced Copper-Temozolomide Interactions by Protein for Chemotherapy against Glioblastoma Multiforme
- Author
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Fenli Shao, Xiao Li, Ke Du, Jian Sun, Yang Sun, and Fude Feng
- Subjects
Materials science ,medicine.medical_treatment ,Antineoplastic Agents ,010501 environmental sciences ,010402 general chemistry ,01 natural sciences ,chemistry.chemical_compound ,Binding efficiency ,Cell Line, Tumor ,medicine ,Temozolomide ,Humans ,General Materials Science ,Prodrugs ,0105 earth and related environmental sciences ,Chemotherapy ,Tumor microenvironment ,Brain Neoplasms ,Recurrent glioblastoma ,Glutathione ,Prodrug ,medicine.disease ,0104 chemical sciences ,chemistry ,Apoferritins ,Cancer research ,Glioblastoma ,Copper ,medicine.drug - Abstract
Current treatment of recurrent glioblastoma multiforme (GBM) demands dose-intense temozolomide (TMZ), a prodrug of 5-(3-methyltriazen-1-yl) imidazole-4-carboxamide (MTIC), based on the spontaneous hydrolysis of TMZ at basic pH. However, how to control the activity of MTIC remains unknown, which poses a particular challenge to search a reliable MTIC receptor. We reported that copper, for the first time, is found to recognize and bind MTIC in the process of TMZ degradation, which means copper can play an important role in enhancing the bioavailability of MTIC derived from TMZ. Using apoferritin as a model copper-bound protein, we studied the copper-TMZ interaction in protein and observed efficient MTIC immobilization with high binding efficiency (up to 92.9% based on original TMZ) and capacity (up to 185 MTIC moieties per protein). The system was stable against both alkaline and acidic pH and could be activated by glutathione to liberate MTIC, which paves a way to deliver a DNA-alkylating agent for both TMZ-sensitive and TMZ-resistant GBM chemotherapy. Our study provides a new insight for understanding the potential relationship between the special GBM microenvironment (specific copper accumulation) and the therapeutic effect of TMZ.
- Published
- 2019
35. Mitochondria Targeting Fluorescent Probes Based on through Bond-Energy Transfer for Mutually Imaging Signaling Molecules H
- Author
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Jian, Sun, Xiao, Li, Jia, Cao, Qi, Sun, Yajie, Zhang, Xuewei, Wang, Tiantian, Wu, Xiantao, Hu, and Fude, Feng
- Abstract
Reactive signaling molecules participate in varieties of biochemical reactions, and methods to detect their mutual existence and crosstalk are in urgent demand. A benzothiadiazole-based handle was designed to fluorescently respond to the co-existence of H
- Published
- 2019
36. Protein Nanocages for Delivery and Release of Luminescent Ruthenium(II) Polypyridyl Complexes
- Author
-
Jian Sun, Yajie Zhang, H. S. Chen, Fude Feng, and Xiao Li
- Subjects
Aqueous solution ,Nanocomposite ,Materials science ,chemistry.chemical_element ,02 engineering and technology ,Protein aggregation ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Photochemistry ,01 natural sciences ,Combinatorial chemistry ,0104 chemical sciences ,Ruthenium ,Nanocages ,chemistry ,Drug delivery ,General Materials Science ,0210 nano-technology ,Luminescence ,Cytotoxicity - Abstract
In this report, noncovalent encapsulation of hydrophobic ruthenium(II) polyridyl complexes, Ru(bpy)2dppz(2+) and Ru(phen)2dppz(2+), into apoferritin cavity was achieved with high loading contents by effective prevention of Ru complex-induced protein aggregation, without disruption of protein native architecture. The Ru-loaded luminescent nanocomposites have demonstrated improved water solubility, easy manipulation, reduced cytotoxicity, and enhanced cellular uptake as compared to the nontreated Ru complexes.
- Published
- 2016
37. A water soluble donor-acceptor-donor conjugated oligomer as a photosensitizer for mitochondria-targeted photodynamic therapy
- Author
-
Jian Sun, Ke Du, Fude Feng, and Xiao Li
- Subjects
medicine.medical_treatment ,Photodynamic therapy ,Antineoplastic Agents ,02 engineering and technology ,Mitochondrion ,Conjugated system ,010402 general chemistry ,01 natural sciences ,Oligomer ,Catalysis ,chemistry.chemical_compound ,Organophosphorus Compounds ,Materials Chemistry ,medicine ,Molecule ,Humans ,Photosensitizer ,Cytotoxicity ,Hypoxia ,Fluorescent Dyes ,Photosensitizing Agents ,Singlet Oxygen ,Singlet oxygen ,Metals and Alloys ,General Chemistry ,Triazoles ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Mitochondria ,chemistry ,Photochemotherapy ,Ceramics and Composites ,Biophysics ,0210 nano-technology ,HeLa Cells - Abstract
We designed a water soluble benzotriazole-cored donor-acceptor-donor molecule bearing four triphenylphosphonium moieties for mitochondria targeting. The organic molecule showed strong singlet oxygen sensitizing capability (ΦΔ 0.64), minimal dark cytotoxicity and high photocytotoxicity. The molecule is efficient in photodynamic killing of tumour cells under normal and hypoxia conditions using a very low light flux, and has potential in near-infrared two-photon imaging and photodynamic therapy.
- Published
- 2018
38. Optical properties of phosphonium-, quaternary ammonium- and imidazolium- substituted regioregular polythiophenes and application for imaging live cells
- Author
-
Ke Du, Fuli Zhang, Cao Guangxiu, Xiaoyu Zheng, Xiang-Fei Zhang, Fude Feng, and Chi Zhang
- Subjects
chemistry.chemical_classification ,Aqueous solution ,Chemistry ,Process Chemistry and Technology ,General Chemical Engineering ,Cationic polymerization ,02 engineering and technology ,Polymer ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Conjugated Polyelectrolytes ,0104 chemical sciences ,chemistry.chemical_compound ,Polymer chemistry ,Side chain ,Ammonium ,Phosphonium ,0210 nano-technology ,Cytotoxicity - Abstract
Cationic side chains on conjugated polyelectrolytes play crucial roles in the state of polymer chains in water as well as the interactions with live cells. However, it is difficult to compare the effect of different cationic side chains due to the structural defect and severe aggregation issue. We synthesized structurally defined cationic polythiophenes with pendent phosphonium-, quaternary ammonium- and imidazolium-substituted side chains, and confirmed molecularly dispersed state of them in aqueous solutions by spectroscopic characterizations. We also investigated the cytotoxicity as well as cell binding and intracellular trafficking by imaging live cells in the absence and presence of hyaluronic acid.
- Published
- 2019
39. Remarkable Amplification of Polyethylenimine-Mediated Gene Delivery Using Cationic Poly(phenylene ethynylene)s as Photosensitizers
- Author
-
Zhiliang Li, Yun Huang, Yajie Zhang, Jinkai Ji, Kirk S. Schanze, Tiantian Wu, Fude Feng, and Hao Yuan
- Subjects
Polyethylenimine ,Materials science ,Photosensitizing Agents ,010405 organic chemistry ,Singlet oxygen ,Polymers ,Cationic polymerization ,Gene Transfer Techniques ,02 engineering and technology ,Conjugated system ,Gene delivery ,021001 nanoscience & nanotechnology ,Transfection ,01 natural sciences ,Combinatorial chemistry ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Phenylene ,DNA supercoil ,Polyethyleneimine ,General Materials Science ,0210 nano-technology ,DNA - Abstract
Conjugated polymers can serve as good photosensitizers in biomedical applications. However, it remains unknown whether they are phototoxic to the supercoiled structure of DNA in improving gene delivery by the photochemical internalization (PCI) strategy, which complicates the application of conjugated polymers in gene delivery. In this work, we introduced a trace amount of cationic poly(phenylene ethynylene)s (cPPEs) into the polymeric shell of branched polyethylenimine (BPEI)/DNA complexes, studied the photosensitization of singlet oxygen by cPPEs, and confirmed that the supercoiled DNA is undamaged by the singlet oxygen generated by the photoexcitation of cPPEs. By taking advantage of the cPPE-mediated PCI effect, we report that the addition of the trace amount of cPPEs to the outer shell of the BPEI/DNA polyplexes could greatly amplify the transfection of gene green fluorescent protein on tumor cells with the efficiency from 14 to 86% without decreasing the cell viabilities, well solving the problem with a poor transfection capability of BPEI under low DNA-loading conditions. Our strategy to employ conjugated polymers as photosensitizing agents in gene delivery systems is simple, safe, efficient, and promising for broad applications in gene delivery areas.
- Published
- 2018
40. Synthesis of Structurally Defined Cationic Polythiophenes for DNA Binding and Gene Delivery
- Author
-
Jinkai Ji, Xiaoyu Zheng, Chi Zhang, Xuewei Wang, Xiaoyan Shi, and Fude Feng
- Subjects
chemistry.chemical_classification ,Condensation polymer ,Materials science ,Polymers ,Cationic polymerization ,02 engineering and technology ,Polymer ,DNA ,Thiophenes ,Gene delivery ,Conjugated system ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Combinatorial chemistry ,0104 chemical sciences ,chemistry.chemical_compound ,Aminolysis ,chemistry ,Cations ,Side chain ,General Materials Science ,Prospective Studies ,0210 nano-technology - Abstract
Water-soluble conjugated polymers (WCPs) have prospective applications in the field of bioimaging, disease diagnosis, and therapy. However, the use of WCPs with controllability and regioregularity for bioapplications have scarcely been reported. In this work, we synthesized polythiophenes containing ester side chains (P3ET) via Kumada catalyst-transfer polycondensation (KCTP) and confirmed a quasi-“living” chain-growth mechanism. In addition, we obtained cationic regioregular polythiophenes (cPTs) by aminolysis of P3ET with varied chain lengths, and studied DNA binding capability and gene delivery performance. Benefiting from photocontrolled generation of intracellular reactive oxygen species (ROS), the cationic polythiophenes successfully delivered DNA into tumor cells without additional polymer species.
- Published
- 2018
41. Targeted Gene Delivery to Macrophages by Biodegradable Star-Shaped Polymers
- Author
-
Yafeng Wang, Fude Feng, Jin Wang, Dejing Pan, Chi Zhang, Yajie Zhang, and Jianghuai Liu
- Subjects
Materials science ,Polymers ,Genetic enhancement ,Mannose ,Nanotechnology ,02 engineering and technology ,Gene delivery ,Transfection ,010402 general chemistry ,01 natural sciences ,Cell Line ,Mice ,chemistry.chemical_compound ,Folic Acid ,Animals ,Moiety ,General Materials Science ,Macrophages ,DNA ,021001 nanoscience & nanotechnology ,Ligand (biochemistry) ,0104 chemical sciences ,chemistry ,Biochemistry ,Cell culture ,0210 nano-technology ,Plasmids - Abstract
In this report, two biodegradable star-shaped polyasparamide derivatives and four analogues modified with either mannose or folic acid moiety for preferential targeting of a difficult-to-transfect immune cell type, i.e., macrophage, have been synthesized. Each of the prepared star polymers complexes with plasmid DNA to form nanosized particles featuring a core-shell-like morphology. Mannose or folate functionalized star polymers can greatly improve the transfection performance on a macrophage cell line RAW 264.7. As a result, a combination of targeting ligand modification and topological structures of gene carriers is a promising strategy for immune cells-based gene therapy.
- Published
- 2015
42. H
- Author
-
Weijian, Chen, Yajie, Zhang, Xiao, Li, Hong, Chen, Jian, Sun, and Fude, Feng
- Subjects
Drug Liberation ,Animals ,Horses ,Hydrogen Sulfide ,Sulfides ,Copper - Abstract
We took advantage of gasotransmitter H
- Published
- 2017
43. Cationic Polythiophenes as Gene Delivery Enhancer
- Author
-
Xiao Li, Tiantian Wu, Fude Feng, Jian Sun, Leilei Cao, Yajie Zhang, and Xuewei Wang
- Subjects
Materials science ,Endolysosome membrane ,Polymers ,Transgene ,Cationic polymerization ,Gene Transfer Techniques ,Nanotechnology ,02 engineering and technology ,Thiophenes ,Gene delivery ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Transfection ,01 natural sciences ,0104 chemical sciences ,chemistry.chemical_compound ,chemistry ,Covalent bond ,Cations ,Biophysics ,Zeta potential ,Polythiophene ,General Materials Science ,0210 nano-technology ,Enhancer - Abstract
There is urgent demand of easily available and highly effective method to improve transgene performance of polymeric gene carriers at low consumption of delivery materials. We developed biocompatible multicomponent nanocomposites in which small quantities of cationic polythiophenes were engineered into the outer shell of polypeptide/DNA polyplexes without covalent linkages. We revealed the introduction of polythiophenes in small quantities led to multiple outcomes including modulation of polyplex size and zeta potential, increase in polyplex stability, promotion of endolysosome membrane disruption, light-induced generation of reactive oxygen species (ROS), and significant enhancement of gene delivery to tumor cells. The factors such as structural architectures, molecular weights, photosensitizing capability, and percentage composition of polythiophenes were investigated.
- Published
- 2017
44. Enhanced Fluorescence Properties of Poly(phenylene ethynylene)-Conjugated Polyelectrolytes Designed to Avoid Aggregation
- Author
-
Jie Yang, Xuzhi Zhu, Fude Feng, Zhenxing Pan, Kirk S. Schanze, and Jan-Moritz Koenen
- Subjects
Aqueous solution ,Materials science ,Polymers and Plastics ,Organic Chemistry ,chemistry.chemical_element ,Photochemistry ,Poly(phenylene ethynylene) ,Fluorescence ,Oxygen ,Conjugated Polyelectrolytes ,Inorganic Chemistry ,chemistry ,Transmission electron microscopy ,Polymer chemistry ,Materials Chemistry ,Spectroscopy - Abstract
A new class of nonaggregating conjugated polyelectrolytes exhibits efficient fluorescence in aqueous solution. Analysis by optical spectroscopy and transmission electron microscopy reveals a unique structure-property correlation between oxygen substitution and aggregation.
- Published
- 2014
45. Efficient synthesis of 5-hydroxymethyl-, 5-formyl-, and 5-carboxyl-2′-deoxycytidine and their triphosphates
- Author
-
Fude Feng, Shouzhi Pu, Shan-Shan Gong, Jian Sun, Cheng-Jun Wang, and Qi Sun
- Subjects
chemistry.chemical_compound ,Chemistry ,Stereochemistry ,5-carboxyl-2'-deoxycytidine ,General Chemical Engineering ,Hydroxymethyl ,General Chemistry - Abstract
An efficient P(V)-N activation strategy for the preparation of high-quality 5-hydroxymethyl-, 5-formyl-, and 5-carboxyl-2′-deoxycytidine triphosphates has been developed. The method was also optimized for gram-scale synthesis of the corresponding parent nucleosides from 2′-deoxythymidine.
- Published
- 2014
46. Facile Synthesis of a Carnosine‐Pendent Cationic Polymer via Free Radical Polymerization and Application in Gene Delivery
- Author
-
Tiantian Wu, Yajie Zhang, Yajing Gao, Xiaoran Zhang, Leilei Cao, Fude Feng, Xuetong Cai, Jinkai Ji, and Xiao Li
- Subjects
chemistry.chemical_compound ,Polymers and Plastics ,chemistry ,Organic Chemistry ,Radical polymerization ,Polymer chemistry ,Materials Chemistry ,Cationic polymerization ,Carnosine ,Physical and Theoretical Chemistry ,Gene delivery ,Condensed Matter Physics - Published
- 2019
47. Precisely Tunable Engineering of Sub-30 nm Monodisperse Oligonucleotide Nanoparticles
- Author
-
M. Neal Waxham, Jianming Xu, Yilong Jia, Antons Sizovs, Xianzhou Song, Fude Feng, Yan Yu, Amanda C. Wicker, Jianwei Chen, and Jin Wang
- Subjects
Cell Survival ,Dispersity ,Oligonucleotides ,Nanoparticle ,Bioengineering ,Polymer architecture ,Nanotechnology ,Calorimetry ,engineering.material ,Biochemistry ,Article ,Catalysis ,Polyethylene Glycols ,Colloid and Surface Chemistry ,Microscopy, Electron, Transmission ,Coating ,Humans ,Particle Size ,Total internal reflection fluorescence microscope ,Molecular Structure ,Oligonucleotide ,Chemistry ,General Chemistry ,Förster resonance energy transfer ,engineering ,Nanoparticles ,Particle size ,HeLa Cells - Abstract
Advancement of RNAi therapies is mainly hindered by the development of efficient delivery vehicles. The ability to create small size (
- Published
- 2013
48. Ion-Induced Aggregation of Conjugated Polyelectrolytes Studied by Fluorescence Correlation Spectroscopy
- Author
-
Danlu Wu, Kirk S. Schanze, Fude Feng, Jie Yang, and Dongping Xie
- Subjects
Quenching (fluorescence) ,Metal ions in aqueous solution ,Diffusion ,Analytical chemistry ,Fluorescence correlation spectroscopy ,Photochemistry ,Conjugated Polyelectrolytes ,Surfaces, Coatings and Films ,Ion ,chemistry.chemical_compound ,chemistry ,Divalent metal ions ,Materials Chemistry ,Carboxylate ,Physical and Theoretical Chemistry - Abstract
Fluorescence correlation spectroscopy (FCS) is applied to investigate aggregation behavior of an anionic conjugated polyelectrolyte (CPE) featuring branched carboxylate groups (PPE-(d)CO2) upon the addition of different metal ions. FCS results reveal that monovalent metal ions (Na(+), K(+)) bind to the CPE chain but do not induce aggregation, and divalent metal ions (Ca(2+), Cu(2+)) bind and induce the formation of small aggregates. Iron cations (Fe(2+) and Fe(3+)) bind strongly and induce the formation of large CPE aggregates. This is believed to be because of the propensity of these metals to bind to six ligands and thus bridge two of the triacid units on adjacent PPE-(d)CO2 chains. A comparison between the Stern-Volmer (SV) fluorescence quenching and the relative diffusion time change from FCS demonstrates that the most efficient SV quenching is observed for the ions that give rise to large increases in FCS diffusion time, underscoring the importance of ion-induced aggregation in the amplified quenching effect.
- Published
- 2013
49. Apoferritin as a Carrier of Cu(II) Diethyldithiocarbamate and Biomedical Application for Glutathione-Responsive Combination Chemotherapy.
- Author
-
Xiao Li, Ke Du, Jian Sun, and Fude Feng
- Published
- 2020
- Full Text
- View/download PDF
50. Remarkable Photophysics and Amplified Quenching of Conjugated Polyelectrolyte Oligomers
- Author
-
Jie Yang, Dongping Xie, Kirk S. Schanze, Tracy D. McCarley, and Fude Feng
- Subjects
chemistry.chemical_compound ,Aqueous solution ,Quenching (fluorescence) ,Phenylene ,Chemistry ,Polymer chemistry ,Cationic polymerization ,General Materials Science ,Fluorescence correlation spectroscopy ,Physical and Theoretical Chemistry ,Conjugated Polyelectrolytes ,Fluorescence ,Oligomer - Abstract
We report the photophysics and fluorescence quenching of a series of monodisperse, anionic π-conjugated oligomers that are molecularly dissolved in aqueous solution. These structurally well-defined oligomers feature oligo(phenylene ethynylene) backbones with two -CH2COO(-) units on each repeat unit, with overall lengths of 5, 7, and 9 repeats. The ionic oligomers display a structured fluorescence band with high quantum efficiency in water, in contrast to the low fluorescence quantum efficiency and pronounced aggregation displayed by structurally similar oligomeric and polymeric (phenylene ethynylene) conjugated polyelectrolytes studied previously. Stern-Volmer (SV) fluorescence quenching studies using cationic charge- and energy-transfer quenchers reveal that all of the oligomers give rise to SV quenching constants (KSV) in excess of 10(6) M(-1), with values increasing with oligomer length, consistent with the amplified quenching effect. The amplified quenching effect is proposed to occur due to the formation of comparatively small oligomer aggregates.
- Published
- 2013
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