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Light-Controlled in Vitro Gene Delivery Using Polymer-Tethered Spiropyran as a Photoswitchable Photosensitizer
- Source :
- ACS Applied Materials & Interfaces. 11:15222-15232
- Publication Year :
- 2019
- Publisher :
- American Chemical Society (ACS), 2019.
-
Abstract
- A gene delivery system using spiropyran as a photoswitchable photosensitizer for the controlled photochemical internalization effect was developed by engineering the outer coating of a polyethylenimine/DNA complex with a small amount of spiropyran-containing cationic copolymers. The successful binding of cationic polymers by the polyethylenimine coating was detected by the distance-sensitive fluorescence resonance energy-transfer technique that evidenced the occurrence of energy transfer between fluorescein-labeled cationic copolymers and polyethylenimine-condensed rhodamine-labeled DNA. The ternary polyplexes feature reversible controllability of singlet oxygen generation based on the dual effect of spiropyrans in photochromism and aggregation-induced enhanced photosensitization, allowing significant light-induced amplification of bPEI-mediated in vitro transgene efficiency (from original 15% to final 91%) at a low DNA dose, with the integrity of supercoiled DNA structure unaffected. The use of spiropyran without the need of other photosensitizers circumvents the issue of uncontrolled long-lasting photocytotoxicity in gene delivery.
- Subjects :
- Indoles
Materials science
Light
Cell Survival
02 engineering and technology
Gene delivery
Transfection
010402 general chemistry
01 natural sciences
chemistry.chemical_compound
Photochromism
Fluorescence Resonance Energy Transfer
Humans
Polyethyleneimine
Benzopyrans
General Materials Science
Photosensitizer
Spiropyran
Polyethylenimine
Photosensitizing Agents
Rhodamines
Singlet oxygen
Cationic polymerization
DNA
Nitro Compounds
021001 nanoscience & nanotechnology
0104 chemical sciences
Microscopy, Fluorescence
chemistry
Biophysics
Nanoparticles
DNA supercoil
Fluorescein
Reactive Oxygen Species
0210 nano-technology
HeLa Cells
Subjects
Details
- ISSN :
- 19448252 and 19448244
- Volume :
- 11
- Database :
- OpenAIRE
- Journal :
- ACS Applied Materials & Interfaces
- Accession number :
- edsair.doi.dedup.....cc691698e2755b6b9f759e2adb7ffa7f