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Precise engineering of apoferritin through site-specific host–guest binding
- Source :
- Chemical Communications. 56:12897-12900
- Publication Year :
- 2020
- Publisher :
- Royal Society of Chemistry (RSC), 2020.
-
Abstract
- The surface engineering of the apoferritin shell by means of traditional chemical modifications usually suffers from site inaccuracy and insufficient conjugation. This report describes a non-covalent method for precise modulation of the apoferritin surface without alteration of amino acid residues. A bifunctional macromolecule, structured as azide-poly(ethylene glycol)-porphyrin (termed TPA), was synthesized. TPA was observed to be able to recognize and bind apoferritin in a 12 : 1 stoichiometry with a higher binding affinity than arachidonate, thanks to the specific host-guest interaction between the pocket of each two-fold channel and the porphyrin moiety. This method allows for site-specific engineering of the apoferritin surface with on demand functionalities and optimization of drug encapsulation.
- Subjects :
- Models, Molecular
Azides
Porphyrins
Surface engineering
Protein Engineering
Catalysis
Polyethylene Glycols
chemistry.chemical_compound
Materials Chemistry
Moiety
Binding site
Bifunctional
Binding Sites
Molecular Structure
Metals and Alloys
General Chemistry
Protein engineering
Combinatorial chemistry
Porphyrin
Surfaces, Coatings and Films
Electronic, Optical and Magnetic Materials
chemistry
Apoferritins
Ceramics and Composites
Ethylene glycol
Macromolecule
Subjects
Details
- ISSN :
- 1364548X and 13597345
- Volume :
- 56
- Database :
- OpenAIRE
- Journal :
- Chemical Communications
- Accession number :
- edsair.doi.dedup.....291c09c50d0ae446caba425c543db08e