113 results on '"Calvo JL"'
Search Results
2. Immunocytochemical localization of basic fibroblast growth factor in the rat pineal gland
- Author
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García-Mauriño Je, J. Boya, Calvo Jl, Francisco López-Muñoz, and Fernando Marin
- Subjects
Male ,Benzylamines ,medicine.medical_specialty ,Neurotoxins ,Basic fibroblast growth factor ,Immunocytochemistry ,Biology ,Pineal Gland ,Pinealocyte ,Immunoenzyme Techniques ,Melatonin ,Neovascularization ,chemistry.chemical_compound ,Pineal gland ,Endocrinology ,Internal medicine ,medicine ,Animals ,Rats, Wistar ,Sympathomimetics ,Denervation ,Sympathectomy, Chemical ,Recombinant Proteins ,Rats ,medicine.anatomical_structure ,chemistry ,Female ,Fibroblast Growth Factor 2 ,medicine.symptom ,Immunostaining ,medicine.drug - Abstract
Marin F, Boya J, Calvo JL, Lopez‐Munoz F, Garcia‐Maurino JE. Immunocytochemical localization of basic fibroblast growth factor in the rat pineal gland. J. Pineal Res. 1994: 16: 44–49. The immunocytochemical localization of basic fibroblast growth factor (b‐FGF) during the postnatal development of the rat pineal gland was studied using a polyclonal antibody against the fraction 1–24 of bovine recombinant b‐FGF. Basic FGF immunoreactivity was evident from day 20 after birth in the endothelial cells and perivascular spaces of the gland. Although b‐FGF immunostaining showed its maximal expression at 30–45 days, it was maintained throughout the entire study period (up to 6 months), mainly in the distal zone of the gland. Pinealocytes did not show b‐FGF immunoreactivity at any time. There were no differences in the localization patterns or the intensity of b‐FGF immunostaining after the prenatal denervation with DSP‐4, a neurotoxic amine. The physiological role of b‐FGF in the adult pineal gland remains unknown; however, it does not seem to play a major role during the cytodifferentiation period of the parenchymal cells, or during the neovascularization in the early postnatal days. Furthermore, its immunocytochemical expression is not affected by the prenatal sympathetic denervation with DSP‐4, in contrast with other neutrophic factors. Copyright © 1994, Wiley Blackwell. All rights reserved
- Published
- 1994
3. Co-expression of glial fabrillary acidic protein and vimentin in reactive astrocytes following brain injury in rats
- Author
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J. Boya, A.L. Carbonell, and Calvo Jl
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Pathology ,medicine.medical_specialty ,Central nervous system ,Vimentin ,macromolecular substances ,Glial Fibrillary Acidic Protein ,medicine ,Animals ,Molecular Biology ,Cerebral Cortex ,biology ,Glial fibrillary acidic protein ,General Neuroscience ,Rats, Inbred Strains ,GFAP stain ,Immunohistochemistry ,Rats ,medicine.anatomical_structure ,nervous system ,Cerebral cortex ,Astrocytes ,Brain Injuries ,Immunology ,biology.protein ,Neuroglia ,Neurology (clinical) ,Developmental Biology ,Astrocyte - Abstract
The immunohistochemical expression of glial fibrillary acidic protein (GFAP) and vimentin (VIM) was studied in reactive astrocytes of the rat cerebral cortex 5 days after a brain injury. Seriated Epon semithin sections were immunostained alternatively for GFAP or VIM. Thereafter, both antigens were detected in consecutive sections of the same cell. Bordering the wound, an inner reactive glial layer 300–350 μm thick, showed positive astrocytes with the two immunohistochemical techniques. In this layer, about 60% of the GFAP positive astrocytes were also positive for VIM. Outside the inner layer, only GFAP positive astrocytes could be found.
- Published
- 1991
4. Microglial Cells in the Central Nervous System of the Rabbit and Rat: Cytochemical Identification Using Two Different Lectins
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A.L. Carbonell, A. Borregón, J. Boya, and Calvo Jl
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Male ,Cerebellum ,Histology ,Central nervous system ,Biology ,Lectins ,medicine ,Animals ,Microglia ,Histocytochemistry ,Ricinus ,Brain ,Lectin ,Rats, Inbred Strains ,biology.organism_classification ,Molecular biology ,Rats ,medicine.anatomical_structure ,Biotinylation ,Immunology ,biology.protein ,Cytochemistry ,Neuroglia ,Female ,Rabbits ,Plant Lectins ,Anatomy ,Biomarkers - Abstract
Microglial cells were selectively demonstrated in the central nervous system of adult rabbits and rats using lectin histochemistry. Biotinylated Ricinus communis agglutinin-120 (RCA-1) and biotinylated Griffonia simplicifolia B4 isolectin (GSA I-B4) were used as histochemical markers on sections of Bouin-fixed paraffin-embedded cerebrum and cerebellum. Results were quite similar using both lectins and both species. GSA I-B4 resulted in a better staining in the rat, while RCA-1 labelling was superior in the rabbit. Neither neurons nor glial cells other than microgha were stained with our technique. Lectin histochemistry applied for the detection of microglial cells appears to be of sufficient selectivity and may be considered as an important tool in the morphological and neurobiological study of these cells.
- Published
- 1991
5. Postnatal Development of the Dog Pineal Gland: Electron Microscopy
- Author
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García-Mauriño Je, J. Boya, A. Lopez-Carbonell, and Calvo Jl
- Subjects
Male ,endocrine system ,medicine.medical_specialty ,Pathology ,Cell type ,Connective tissue ,Biology ,Pineal Gland ,law.invention ,Pinealocyte ,Melatonin ,chemistry.chemical_compound ,Pineal gland ,Dogs ,Nerve Fibers ,Endocrinology ,law ,Internal medicine ,medicine ,Animals ,Glycogen ,Cell Differentiation ,medicine.anatomical_structure ,nervous system ,chemistry ,Astrocytes ,Ultrastructure ,Female ,Electron microscope ,Cell Division ,hormones, hormone substitutes, and hormone antagonists ,medicine.drug - Abstract
The ultrastructure of the dog pineal gland from the first postnatal day to the seventh month is described. In the first postnatal stages, pineal parenchyma only shows immature proliferative cells with abundant cytoplasmic glycogen. Nerve fibers are seen in the pineal connective tissue spaces. The differentiation of the dog pineal cell types begins in the first postnatal week. Both pinealocytes and pigmented cells are first seen on the fourth postnatal day. The pineal astrocytes are observed on the tenth day. Immature cells are still found in the pineal gland of 1 mo-old dogs. The differentiation of the dog pineal cell types is completed by the second postnatal month.
- Published
- 1990
6. Coexpression of vimentin and glial fibrillary acidic protein in astrocytes of the adult rat optic nerve
- Author
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J. Boya, A.L. Carbonell, and Calvo Jl
- Subjects
Pathology ,medicine.medical_specialty ,Cell Survival ,Cell ,Central nervous system ,Nerve Tissue Proteins ,Vimentin ,macromolecular substances ,Immunoenzyme Techniques ,Glial Fibrillary Acidic Protein ,polycyclic compounds ,medicine ,Animals ,Molecular Biology ,Glial fibrillary acidic protein ,biology ,General Neuroscience ,Optic Nerve ,Rats, Inbred Strains ,Anatomy ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,GFAP stain ,Rats ,medicine.anatomical_structure ,nervous system ,Astrocytes ,biology.protein ,Optic nerve ,Immunohistochemistry ,Neurology (clinical) ,Developmental Biology ,Astrocyte - Abstract
The localization of vimentin (VIM) and glial fibrillary acidic protein (GFAP) was studied immunohistochemically in adult rat optic nerve. Consecutive Epon semithin tissue sections were immunostained respectively for VIM or GFAP. With this method, both antigens were detected in consecutive sections of the same cell. According to our results, most astrocytes in the adult rat optic nerve showed coexpression of VIM and GFAP.
- Published
- 1990
7. Influence of the light and dark phase of the cycle on the cellular proliferation in the pineal gland of the adult rat: a bromodeoxyuridine immunohistochemical study
- Author
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Angel L. Carbonell, García-Mauriño Je, J. Boya, and Calvo Jl
- Subjects
Male ,endocrine system ,medicine.medical_specialty ,Cell division ,Photoperiod ,Biology ,Pineal Gland ,Pinealocyte ,Melatonin ,chemistry.chemical_compound ,Pineal gland ,Endocrinology ,Internal medicine ,medicine ,Animals ,Circadian rhythm ,Rats, Wistar ,photoperiodism ,DNA ,Immunohistochemistry ,Rats ,medicine.anatomical_structure ,chemistry ,Bromodeoxyuridine ,sense organs ,hormones, hormone substitutes, and hormone antagonists ,Cell Division ,Endocrine gland ,medicine.drug - Abstract
The cellular proliferative activity in the adult rat pineal gland was studied using bromodeoxyuridine immunohistochemistry during the light and dark phases of an artificial 12L:12D photoperiod. The results showed statistically significant differences in the number of labeled cells between the light and dark phases, with the labeled cells being almost threefold more abundant in the light period. Minor changes were also found in the pineal gland volume between both periods. The decrease in the number of labeled cells in the dark phase of the cycle could be related to the well-documented antimitotic action of melatonin.
- Published
- 1997
8. Scientific research on the pineal gland and melatonin: a bibliometric study for the period 1966-1994
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Fernando Marin, Calvo Jl, J. Boya, and Francisco López-Muñoz
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Publishing ,medicine.medical_specialty ,Index (economics) ,business.industry ,MEDLINE ,Research ,Subject (documents) ,Scientific literature ,Bibliometrics ,Pineal Gland ,Pineal gland ,Endocrinology ,medicine.anatomical_structure ,Internal medicine ,medicine ,Humans ,Social science ,business ,Productivity ,Period (music) ,Melatonin - Abstract
By means of teledischarge techniques from the database MEDLINE we selected those documents that contained in their title one or several of the following descriptors: pineal*, epiphys*, or melatonin*, in addition to the descriptor pineal-body in the MESH (Medical Subject Headings) section. A total of 7,617 original documents published between 1966 and 1994 were extracted that dealt with any aspect related with the pineal gland or its main secretary product, melatonin. The main bibliometric laws were applied: Price's Law on the increase in scientific literature, Bradford's Law on the dispersion of the scientific literature, and Lotka's Law on the author's productivity. Furthermore, we have analyzed the participation index (PaI) of the main countries within the global production, the productivity index of the authors (PI), and the number of authors/paper index. Our results demonstrate an exponential increase of the scientific literature on the pineal gland ("r" value = 0.983, in contrast with a "r" value = 0.966 after the linear adjustment). The number of publications on melatonin was less than those on other aspects of pineal research until 1991, when this situation was reversed. The journal with the largest number of original papers is Journal of Pineal Research (1st Bradford's zone) with 533 articles, followed by Journal of Neural Transmission (258) and Neuroendocrinology (221), which constituted the 2nd Bradford's zone. The total number of authors is 9,140, responsible for 23,524 authorships. 3.8% of the authors present a PI > or = 1 (large producers), and 64.9% a PI = 0 (occasional authors). Lotka's Law was widely fulfilled in this material since 10.3% of the authors are responsible of 50.2% of all the papers. The average number of authors per paper has changed from 2.29 in 1966 to 3.85 in 1994. The most productive country (during the interval between 1988-1994) was USA (PaI = 30.6), followed by Japan (7.15), United Kingdom (6.45), Germany (6.37), France (6.26), Italy (6.15) and Spain (5.34). Of the total number articles published, 86.9% are in English.
- Published
- 1996
9. Structure of the pineal gland in the adult cat
- Author
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Calvo Jl, Dolores Rancano, and J. Boya
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Male ,Myelinated nerve fiber ,Unmyelinated nerve fiber ,Connective tissue ,Golgi Apparatus ,Anterior commissure ,Biology ,Endoplasmic Reticulum ,Microtubules ,Pineal Gland ,Pinealocyte ,symbols.namesake ,Pineal gland ,Endocrinology ,Nerve Fibers ,medicine ,Animals ,Centrioles ,Anatomy ,Golgi apparatus ,Mitochondria ,medicine.anatomical_structure ,nervous system ,Astrocytes ,symbols ,Ultrastructure ,Cats ,Female ,Ribosomes ,Cell Nucleolus - Abstract
The ultrastructure of the pineal gland in the adult cat is described and compared with that of other mammals. Connective tissue spaces showed capillaries with nonfenestrated endothelia and numerous unmyelinated nerve fibers. In the proximal region of the gland, myelinated nerve fibers coming from the anterior commissure were also found. Cat pinealocytes showed a nucleus with prominent nucleoli, a well developed Golgi apparatus, centrioles, granular endoplasmic reticulum, ribosomes, abundant microtubuli and enlarged mitochondria. Pinealocytes showed several long processes with bulbous endings filled with clear vesicles and scarce "synaptic" ribbons. Pineal astrocytes and their processes were characterized by the presence of abundant filaments.
- Published
- 1995
10. Immunohistochemical study of the pineal astrocytes in the postnatal development of the cat and dog pineal gland
- Author
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Calvo Jl and J. Boya
- Subjects
Male ,medicine.medical_specialty ,Pathology ,Population ,Vimentin ,macromolecular substances ,Pineal Gland ,Immunoenzyme Techniques ,Pineal gland ,Endocrinology ,Dogs ,Internal medicine ,Glial Fibrillary Acidic Protein ,polycyclic compounds ,medicine ,Animals ,education ,education.field_of_study ,Glial fibrillary acidic protein ,biology ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,GFAP stain ,medicine.anatomical_structure ,nervous system ,Astrocytes ,biology.protein ,Cats ,Neuroglia ,Female ,Endocrine gland ,Astrocyte - Abstract
The expression of glial antigens vimentin (VIM) and glial fibrillary acidic protein (GFAP) is described in the pineal gland of cats and dogs from the first postnatal days to adulthood. VIM immunopositive cells were observed from the first postnatal days in both species. GFAP expression starts from the second postnatal week. In adults, a notable population of stellate cells immunopositive for GFAP and VIM was found dispersed throughout the gland. According to their immunocytochemical profile, these cells could be identified as astrocytes.
- Published
- 1993
11. Immunohistochemical study of the pineal glial cells in the postnatal development of the rat pineal gland
- Author
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Antonio Borregón, Calvo Jl, J. Boya, and Francisco López-Muñoz
- Subjects
Male ,endocrine system ,medicine.medical_specialty ,Vimentin ,Pineal Gland ,Melatonin ,Immunoenzyme Techniques ,Pineal gland ,Endocrinology ,Internal medicine ,Glial Fibrillary Acidic Protein ,medicine ,Animals ,Rats, Wistar ,Glial fibrillary acidic protein ,biology ,S100 Proteins ,Rats ,medicine.anatomical_structure ,nervous system ,biology.protein ,Immunohistochemistry ,Neuroglia ,Female ,Immunostaining ,medicine.drug ,Endocrine gland - Abstract
The developmental expression of the glial antigens, vimentin (VIM), glial fibrillary acidic protein (GFAP), and S-100 protein is described in the rat pineal gland from the first postnatal day to adulthood. Thick VIM immunopositive cell cords forming a network throughout the pineal gland were observed from the first postnatal days. These cords progressively disappeared during the first postnatal month as their cells dispersed into the pineal parenchyma. From 20 to 25 postnatal days, pineal glial cells appeared as isolated star-shaped VIM immunopositive cells. Immunostaining for GFAP and S-100 protein showed a similar developmental expression pattern. Both antigens appeared later than VIM (15–20 postnatal days) and were restricted to the pineal glial cells located in the proximal third of the gland, close to the pineal stalk.
- Published
- 1993
12. Immunohistochemical localization of nerve growth factor in the rat pineal gland
- Author
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García-Mauriño Je, J. Boya, Calvo Jl, and Francisco López-Muñoz
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endocrine system ,medicine.medical_specialty ,Population ,Pineal Gland ,Pineal gland ,Internal medicine ,medicine ,Animals ,Tissue Distribution ,Nerve Growth Factors ,education ,Oxidopamine ,Molecular Biology ,Antiserum ,education.field_of_study ,biology ,Staining and Labeling ,General Neuroscience ,Sympathectomy, Chemical ,Rats, Inbred Strains ,Immunohistochemistry ,Rats ,Endocrinology ,medicine.anatomical_structure ,Nerve growth factor ,nervous system ,Polyclonal antibodies ,Cervical ganglia ,biology.protein ,Neurology (clinical) ,Developmental Biology ,Endocrine gland - Abstract
Sympathetic nerve fibers arising from the superior cervical ganglia are the main innervation of the rat pineal gland. Since most organs innervated by these ganglia contain nerve growth factor (NGF), the hypothetical existence of NGF in the pineal gland was investigated. The peroxidase anti-peroxidase technique was applied for the immunohistochemical demonstration of NGF using a polyclonal antiserum on Bouin-fixed, paraffin-embedded pineal glands from adult, young and 6-hydroxydopamine (6-OHDA)-treated rats. Few immunopositive cells were observed in the adult pineal gland. A more conspicuous population of immunoreactive cells was noted in young animals (20-45 days old), especially in those chemically denervated with 6-OHDA. NGF immunoreactive cells displayed a stellate shape resembling the interstitial or glial cells previously described in the rat pineal gland. Since NGF plays a trophic effect on sympathetic neurons during development and adulthood, we postulate that its presence in the pineal gland may exert a trophic role on its sympathetic innervation.
- Published
- 1992
13. Coexpression of vimentin and glial fibrillary acidic protein in glial cells of the adult rat pineal gland
- Author
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Francisco López-Muñoz, Angel L. Carboneil, Calvo Jl, and J. Boya
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Male ,endocrine system ,medicine.medical_specialty ,Vimentin ,macromolecular substances ,Pineal Gland ,Melatonin ,Immunoenzyme Techniques ,Pineal gland ,Endocrinology ,Internal medicine ,Glial Fibrillary Acidic Protein ,medicine ,Animals ,Rats, Wistar ,Glial fibrillary acidic protein ,biology ,biochemical phenomena, metabolism, and nutrition ,bacterial infections and mycoses ,GFAP stain ,Molecular biology ,Rats ,medicine.anatomical_structure ,nervous system ,biology.protein ,Immunohistochemistry ,Neuroglia ,Female ,Endocrine gland ,medicine.drug - Abstract
In the present work, coexpression of vimentin (VIM) and glial fibrillary acidic protein (GFAP) is demonstrated in the glial cells of the adult rat pineal gland. Serial consecutive Epon semithin sections (0.5 microns thick) were alternately immunostained for VIM and GFAP. GFAP positive cells and processes were found in the proximal region of the pineal gland, near the pineal stalk. Most of these cells were also immunostained for VIM in adjacent semithin sections. The significance of the coexpression VIM-GFAP and the restricted location of GFAP positive cells is discussed in relation with the maturation of pineal glial cells.
- Published
- 1992
14. Presence of melanin in the cat pineal gland
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García-Mauriño Je, Calvo Jl, J. Boya, and D. Rancano
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Male ,endocrine system ,medicine.medical_specialty ,Pathology ,Silver Staining ,Histology ,Pineal Gland ,Melanin ,Pineal gland ,Internal medicine ,medicine ,Carnivora ,Animals ,Postnatal day ,Melanins ,CATS ,biology ,Fissipedia ,biology.organism_classification ,Microscopy, Electron ,medicine.anatomical_structure ,Endocrinology ,Ultrastructure ,Cats ,Female ,sense organs ,Anatomy - Abstract
Light- and electron-microscopic features of pigmented cells in the cat pineal gland are described. These cells were observed throughout postnatal life from the second postnatal day to the oldest cats studied (up to 13 years old). No apparent relationship was observed among the amount of pigment and the animal age or sex. Pigmented cells showed a preferential localization at the ventral surface of the pineal gland near its distal end. The pineal pigment was histochemically identified as melanin. Pineal pigment granules showed ultrastructural features similar to melanocyte melanin granules.
- Published
- 1992
15. Appearance of microglial cells in the postnatal rat retina
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Calvo Jl, J. Boya, and A.L. Carbonell
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Pathology ,medicine.medical_specialty ,Histology ,Acid Phosphatase ,Outer plexiform layer ,Rat retina ,Retina ,chemistry.chemical_compound ,medicine ,Animals ,Postnatal day ,biology ,Microglia ,Histocytochemistry ,Acid phosphatase ,Retinal ,Rats ,medicine.anatomical_structure ,Animals, Newborn ,chemistry ,Immunology ,biology.protein ,Blood Vessels ,sense organs ,Anatomy - Abstract
The development of microglial cells in the postnatal rat retina is described using histochemical techniques for acid phosphatase and peroxidase as well as silver impregnations for microglia. On the second postnatal day, round acid phosphatase-positive macrophages appeared on the vitreal surface of retina, locating themselves close to developing blood vessels. Later, microglial precursors invaded retinal tissues, reaching the outer plexiform layer by the tenth postnatal day. In all stages studied, microglia or their precursors were peroxidase-negative. The transformation of round microglial precursors into adult ramified microglia is also described. Owing to the relation found between developing microglia and blood vessels, a vascular origin is proposed for the retinal microglial cells.
- Published
- 1987
16. Nature of Macrophages in Rat Brain
- Author
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E. Garcia-Mauriño, J. Boya, A.L. Carbonell, and Calvo Jl
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Pathology ,medicine.medical_specialty ,Histology ,Microglia ,Monocyte ,Central nervous system ,Acid phosphatase ,Endogeny ,Biology ,medicine.anatomical_structure ,Immunology ,biology.protein ,medicine ,Immunohistochemistry ,Macrophage ,Anatomy ,Peroxidase - Abstract
The nature of phagocytes appearing in lesions of the central nervous system is strongly debated with a tendency to assess an exclusively hematogenous origin. We studied the origin of phagocytes appearing in a stab wound in the rat brain. Histochemical stains for acid phosphatase and peroxidase, and silver impregnation techniques were used for our study. The results obtained showed the existence of two macrophage types: endogenous microghocytes and exogenous monocytes.
- Published
- 1986
17. Ultrastructure of the Pineal Gland in the Adult Dog
- Author
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J. Boya, E. Garcia-Mauriño, and Calvo Jl
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Male ,Cytoplasm ,endocrine system ,Pathology ,medicine.medical_specialty ,Unmyelinated nerve fiber ,Connective tissue ,Biology ,Pineal Gland ,Pinealocyte ,law.invention ,Pineal gland ,symbols.namesake ,Dogs ,Nerve Fibers ,Endocrinology ,law ,medicine ,Animals ,Centrioles ,Cell Nucleus ,Organelles ,Anatomy ,Golgi apparatus ,Axons ,Actin Cytoskeleton ,Microscopy, Electron ,medicine.anatomical_structure ,nervous system ,Astrocytes ,symbols ,Ultrastructure ,Female ,Electron microscope ,Cell Nucleolus ,Endocrine gland - Abstract
The adult dog pineal gland was studied with the electron microscope. Pineal connective tissue spaces were poorly developed and showed capillaries with nonfenestrated endothelial cells. Two cell types, pinealocytes and astrocytes, could be identified in pineal parenchyma. Dog pinealocytes showed microtubules, centrioles, occasional cilia, and well-developed Golgi complexes. These cells showed thin processes with bulbous endings packed with vesicles. Astrocytes were characterized by the presence of numerous filaments. Their processes finished forming a glial layer bordering connective tissue spaces. The presence of myelinated and unmyelinated nerve fibers was also described.
- Published
- 1988
18. Presence of glial cells in the rat pineal gland: A light and electron microscopic immunohistochemical study
- Author
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García-Mauriño Je, J. Boya, A. Borregon, and Calvo Jl
- Subjects
endocrine system ,Cell type ,Pathology ,medicine.medical_specialty ,Immunoperoxidase ,biology ,Glial fibrillary acidic protein ,Vimentin ,GFAP stain ,Agricultural and Biological Sciences (miscellaneous) ,law.invention ,nervous system ,law ,medicine ,biology.protein ,Ultrastructure ,Immunohistochemistry ,Anatomy ,Electron microscope - Abstract
Immunoperoxidase methods for the demonstration of three glial antigens, vimentin, glial fibrillary acidic protein, and S-100 protein, were applied to routine-fixed paraffin sections of rat pineal gland. A pre-embedding electron microscope immunoperoxidase method was also used to study the ultrastructural localization of S-100 protein in pineal cells. Light and electron microscopic results showed the presence of these antigenic glial markers in the second pineal cell type. The term glial cell is proposed for the second of parenchymatous cell in rat pineal gland.
- Published
- 1988
19. Ultrastructural study on the origin of rat microglia cells
- Author
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Calvo Jl, J. Boya, A.L. Carbonell, and A. Borregón
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Pathology ,medicine.medical_specialty ,Histology ,Microglia ,Central nervous system ,Brain ,Biology ,Capillaries ,Rats ,Microscopy, Electron ,medicine.anatomical_structure ,Fetus ,Meninges ,Animals, Newborn ,Cerebral cortex ,Adventitia ,Parenchyma ,Immunology ,medicine ,Ultrastructure ,Neuroglia ,Macrophage ,Animals ,Anatomy ,Nerve Tissue - Abstract
An ultrastructural study of the origin of microglial cells has been performed in albino rat brains taken from 17-day-old embryos up to 35-day-old rats. Invasion of the nervous parenchyma by macrophagic cells which appear in mesodermal sources is described. Although the two main microglial sources are the meningeal membranes and the vascular adventitia, pericytes may also participate in the formation of microglial cells.
- Published
- 1987
20. Structure and ultrastructure of the aging rat pineal gland
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Calvo Jl and J. Boya
- Subjects
Male ,endocrine system ,medicine.medical_specialty ,Pathology ,Aging ,Connective tissue ,Biology ,Pineal Gland ,law.invention ,Pinealocyte ,Lipofuscin ,Melatonin ,Pineal gland ,Endocrinology ,law ,Internal medicine ,medicine ,Animals ,Rats, Inbred Strains ,Rats ,Microscopy, Electron ,medicine.anatomical_structure ,Oxytalan ,Ultrastructure ,Female ,Electron microscope ,medicine.drug - Abstract
The pineal gland of rats 12-28 months old was studied with light and electron microscopes. All pineal components exhibited regressive changes of different intensity with age. In type I pinealocytes, there was a marked increase in dense bodies as well as the occasional appearance of wide cell profiles full of vesicles. Type II pinealocytes showed nuclear infoldings and cytoplasmic deposits of lipofuscin. Pineal stroma displayed an increase in connective tissue fibers, both collagen and oxytalan, as well as remains of basement membranes and other materials of unknown nature. Calcareous concretions were also found, mostly in the pineal capsule. All regressive changes were more intense with increasing age.
- Published
- 1984
21. How Political Illusions Harm National Stability: Fiscal Illusion as a Source of Taxation
- Author
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Paulo Mourao, Cabral, Jose Pedro, Herrero, A., Snasel, V., Abraham, A., Zelinka, I., Baruque, B., Quintian, H., Calvo, Jl, Sedano, J., and Corchado, E.
22. An Interactive Genetic Algorithm for the Unequal Area Facility Layout Problem
- Author
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Laura Garcia-Hernandez, Salas Morera, Lorenzo, Arauzo Azofra, Antonio, Corchado, E., Snasel, V., Sedano, J., Hassanien, Ae, Calvo, Jl, and Slezak, D.
23. Subject Index, Vol. 130, 1987
- Author
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Michael J. Ulissey, A. Borregón, Klaus Fischer, P.E. Spoerri, J.A. Garcís-Porrero, Jack Davies, Elisabeth Vogel, G.J. Hausman, Christopher R. Murphy, J. More, Calvo Jl, A. Amara, Judy Lefkowitz, Michael E. Trulson, Margot J. Hosie, B. Rama, J. Sautet, J. Leeton, A.L. Carbonell, Peter Rogers, M.A. Ros, Stefan Nachbaur, J.L. Ojeda, Gabriele M. Rune, Johannes Lang, Tatsumi Nakano, L. Beaton, J. Boya, C.J. Catalina-Herrera, Christian Hack, Eduardo Couve, G. Van Haverbeke, R.L. Richardson, Anne M. Macpherson, Y. Ogawa, H. Muto, and P. Cabanie
- Subjects
Histology ,Index (economics) ,Statistics ,Subject (documents) ,Anatomy ,Mathematics - Published
- 1987
24. Nanocrystalline Cellulose to Reduce Superplasticizer Demand in 3D Printing of Cementitious Materials.
- Author
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Jarabo R, Fuente E, García Calvo JL, Carballosa P, and Negro C
- Abstract
One challenge for 3D printing is that the mortar must flow easily through the printer nozzle, and after printing, it must develop compressive strength fast and high enough to support the layers on it. This requires an exact and difficult control of the superplasticizer (SP) dosing. Nanocrystalline cellulose (CNC) has gained significant interest as a rheological modifier of mortar by interacting with the various cement components. This research studied the potential of nanocrystalline cellulose (CNC) as a mortar aid for 3D printing and its interactions with SPs. Interactions of a CNC and SP with cement suspensions were investigated by means of monitoring the effect on cement dispersion (by monitoring the particle chord length distributions in real time) and their impact on mortar mechanical properties. Although cement dispersion was increased by both CNC and SP, only CNC prevented cement agglomeration when shearing was reduced. Furthermore, combining SP and CNC led to faster development of compressive strength and increased compressive strength up to 30% compared to mortar that had undergone a one-day curing process.
- Published
- 2024
- Full Text
- View/download PDF
25. Basketball talent identification: a systematic review and meta-analysis of the anthropometric, physiological and physical performance factors.
- Author
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Han M, Gómez-Ruano MA, Calvo AL, and Calvo JL
- Abstract
Background: The purpose of this study was to do a systematic review and meta-analysis about the anthropometric, physiological, and physical performance that discriminate the physical talent in basketball., Methods: The search was conducted using the most relevant databases as Web of Science, PubMed, SPORTDiscus and Scopus, according to the PRISMA (2020) guideline. Keywords such as "basketball", "talented identification", "anthropometric", "physiology" and "physical performance" were used for the search, in English and following the "PICOS" question model. Eighteen articles' overall methodological quality was assessed using the Critical Review Forms., Results: The study found in basketball, height, body mass, Yo-Yo test, T -test, 20 m sprint, and jump performance had statistical significance between elite and non-elite groups, including different positions and levels., Conclusions: The reviewed literature highlighted a complicated relationship between anthropometric, physiological, and physical performance. Based on the results of the study, it's possible that height, body mass, agility, speed, endurance capacity, and lower lamb power could affect the early development of basketball., Competing Interests: The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest. The author(s) declared that they were an editorial board member of Frontiers, at the time of submission. This had no impact on the peer review process and the final decision., (© 2023 Han, Gómez-Ruano, Calvo and Calvo.)
- Published
- 2023
- Full Text
- View/download PDF
26. Development of Lightweight Mortars Using Sustainable Low-Density Glass Aggregates from Secondary Raw Materials.
- Author
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Romero M, Padilla I, García Calvo JL, Carballosa P, Pedrosa F, and López-Delgado A
- Abstract
In this study, different lightweight expanded glass aggregates (LEGAs) were produced from glass cullet and various carbonated wastes, through a thermal impact process. The effects of LEGA microstructure and morphology on both the adherence to the cement paste and the mechanical properties of mortars after 28 days of curing were studied. The properties of lightweight mortars made of either LEGAs or expanded clay aggregates were compared. The results demonstrated the feasibility of using LEGAs to produce glass lightweight aggregate mortar, with flexural and compressive strength values ranging from 5.5 to 8.2 MPa and from 28.1 to 47.6 MPa, respectively. The differences in mechanical properties were explained according to the microstructures of the fracture surfaces. Thus, arlite-type ceramic aggregates presented surface porosities that allowed mortar intrusion and the formation of an interconnected interface; although the surfaces of the vitreous aggregates were free from porosity due to their vitreous nature, the mortars obtained from different wastes presented compressive and flexural strengths in the range of lightweight mortars.
- Published
- 2023
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27. Colloidal Nanosilica Treatments for Sealing Cracks in Mortar.
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Sánchez-Moreno M, García-Calvo JL, and Tavares-Pinto F
- Abstract
Presence of microcracks in concrete can diminish the service life of a structure. The injection of materials for filling the crack is proposed for facing this problem. The traditional materials used for sealing cracks present some drawbacks, such as the difficulties of inorganic materials for flowing to all the depth of the crack and the lack of compatibility with the cementitious matrix in the case of organic materials. In this work, the injection of colloidal nanosilica dispersed in water is proposed for filling microcracks in mortars. The effect of the injection procedure on the sealing performance of the colloidal nanosilica has been assessed. The ability of colloidal nanosilica for penetrating through the crack and its posterior gelification-solidification inside the crack after a curing period have been confirmed. The microscopic analysis of a cross-section of the crack indicates that the sealing ability of the nanosilica seems to be not only due to the filling of the crack but also to chemical interactions with the cementitious phases of the surrounding crack sides.
- Published
- 2022
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28. Does predicted age at peak height velocity explain physical performance in U13-15 basketball female players?
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Gryko K, Adamczyk JG, Kopiczko A, Calvo JL, Calvo AL, and Mikołajec K
- Abstract
Background: The aims of the study were (1) to identify the physical fitness and basic anthropometric characteristics of Polish female basketball players aged 13-15 years, (2) to show the effect of maturity timing on the performance in motor tests and basic body composition parameters, (3) to identify the index that contributes most to the prediction of performance in the tests of speed, jumping ability, agility, and endurance., Methods: The sample included 904 female Polish players (U13-15). In part 1, maturity timing category distribution were examined within across age-groups. Maturity timing was followed by grouping with respect to years before or after the observed peak high velocity (PHV): PHV0 (- 0.50 to 0.49), PHV1 (0.50 to 1.49), PHV2 (1.50 to 2.49) and PHV3 (2.50 to 3.49). In part 2, the relationship between the anthropometric variables, physical fitness performance was assessed based on maturity timing categories (ANCOVA analysis). In part 3, backward stepwise multiple regression analyse quantified the relationship between maturity timing (group of PHV) and physical performance., Results: ANCOVA results (age, body height, and body mass as covariates) showed in the U13 female basketball players significantly higher sprinting (20 m), jumping ability and endurance tests results of the PHV1 group. Better results was observed in U14 female players in PHV1 compared to PHV2 and PHV3 in 20 m and jumping tests but opposite trend was observed for 5 m sprint and endurance test (distance covered and VO2
max ). U15 basketball players from the PHV3 group were characterized by better results of jumping abilities, endurance, 10 m and 20 m sprint and agility (total, S4 ) tests. Maturity timing (10 m), chronological age (5 m, 20 m, agility, SVJ, VJ, and VO2max tests), body height (10 m), body mass (10 m, 20 m, VJ, VO2max ), and the interaction between body mass and height (SVJ) were significant (adjusted R2 = 0.02-0.10; p < 0.001) predictors of motor skills., Conclusion: Trainng content of female basketball players aged 13-15 years old should be adjusted to biological requirements especially in jumping, endurance and 20 m sprint test. The time from peak height velocity (PHV) was a significant predictor only in the 10 m sprint test., (© 2022. The Author(s).)- Published
- 2022
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29. Effects of Exercise Programs on Physical Factors and Safety in Adult Patients with Cancer and Haematopoietic Stem Cell Transplantation: A Systematic Review.
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Morales-Rodriguez E, Pérez-Bilbao T, San Juan AF, and Calvo JL
- Subjects
- Exercise, Exercise Therapy, Humans, Muscle Strength physiology, Quality of Life, Hematopoietic Stem Cell Transplantation, Neoplasms therapy
- Abstract
This study looks at the effects of exercise programs on physical factors and safety in adult patients with cancer and hematopoietic stem cell transplantation (HSCT) or bone marrow transplantation (BMT). A systematic search was performed in the PubMed and Web of Science databases (from inception to 26 August 2021). A review was carried out following the Preferred Reporting Items for Systematic review and Meta-Analyses (PRISMA) checklist. The methodological quality of the included studies was assessed with the Physiotherapy Evidence Database (PEDro) scale, based, in turn, on the Delphi list. A total of 25 randomized controlled trials studies were included, comprising 1434 patients. The most significant result of this systematic review is that exercise program interventions are safe and produce positive changes in cardiorespiratory fitness, muscle strength, and the functional mobility-state in adult patients with cancer and HSCT or BMT. Only 3 patients from the 711 participants in the exercise interventions (i.e., 0.42%) reported adverse events related to exercise interventions. Moreover, exercise training programs may have a cardiological and muscular protective effect, as well as a healthy effect on the prevention and control of transplant complications, improving health outcomes.
- Published
- 2022
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30. Residual Strength and Drying Behavior of Concrete Reinforced with Recycled Steel Fiber from Tires.
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Revuelta D, Carballosa P, García Calvo JL, and Pedrosa F
- Abstract
Fiber reinforcement of concrete is an effective technique of providing ductility to concrete, increasing its flexural residual strength while reducing its potential for cracking due to drying shrinkage. There are currently a wide variety of industrial fibers on the market. Recycled steel fibers (RSF) from tires could offer a viable substitute of industrialized fibers in a more sustainable and eco-friendly way. However, mistrust exists among users, based on fear that the recycling process will reduce the performance, coupled with the difficulty of characterization of the geometry of the RSF, as a consequence of the size variability introduced by the recycling process. This work compares the behavior of RSF from tires compared with industrialized steel or polypropylene fibers, evaluating the fresh state, compressive strength, flexural residual strength, and drying behavior. The concept of Equivalent Fiber Length (EFL) is also defined to help the statistical geometrical characterization of the RSF. A microstructural analysis was carried out to evaluate the integration of the fiber in the matrix, as well as the possible presence of contaminants. The conclusion is reached that the addition of RSF has a similar effect to that of industrialized fibers on concrete's properties when added at the same percentage.
- Published
- 2021
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31. Evaluation of Methodologies for Assessing Self-Healing Performance of Concrete with Mineral Expansive Agents: An Interlaboratory Study.
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Litina C, Bumanis G, Anglani G, Dudek M, Maddalena R, Amenta M, Papaioannou S, Pérez G, García Calvo JL, Asensio E, Beltrán Cobos R, Tavares Pinto F, Augonis A, Davies R, Guerrero A, Sánchez Moreno M, Stryszewska T, Karatasios I, Tulliani JM, Antonaci P, Bajare D, and Al-Tabbaa A
- Abstract
Self-healing concrete has the potential to optimise traditional design approaches; however, commercial uptake requires the ability to harmonize against standardized frameworks. Within EU SARCOS COST Action, different interlaboratory tests were executed on different self-healing techniques. This paper reports on the evaluation of the effectiveness of proposed experimental methodologies suited for self-healing concrete with expansive mineral additions. Concrete prisms and discs with MgO-based healing agents were produced and precracked. Water absorption and water flow tests were executed over a healing period spanning 6 months to assess the sealing efficiency, and the crack width reduction with time was monitored. High variability was reported for both reference (REF) and healing-addition (ADD) series affecting the reproducibility of cracking. However, within each lab, the crack width creation was repeatable. ADD reported larger crack widths. The latter influenced the observed healing making direct comparisons across labs prone to errors. Water absorption tests highlighted were susceptible to application errors. Concurrently, the potential of water flow tests as a facile method for assessment of healing performance was shown across all labs. Overall, the importance of repeatability and reproducibility of testing methods is highlighted in providing a sound basis for incorporation of self-healing concepts in practical applications.
- Published
- 2021
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32. Effect of sodium bicarbonate contribution on energy metabolism during exercise: a systematic review and meta-analysis.
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Calvo JL, Xu H, Mon-López D, Pareja-Galeano H, and Jiménez SL
- Subjects
- Anaerobiosis drug effects, Anaerobiosis physiology, Bicarbonates metabolism, Carbon Dioxide metabolism, Energy Metabolism physiology, Humans, Hydrogen-Ion Concentration drug effects, Lactic Acid blood, Oxygen metabolism, Sodium Bicarbonate administration & dosage, Energy Metabolism drug effects, Exercise physiology, Sodium Bicarbonate pharmacology
- Abstract
Background: The effects of sodium bicarbonate (NaHCO
3 ) on anaerobic and aerobic capacity are commonly acknowledged as unclear due to the contrasting evidence thus, the present study analyzes the contribution of NaHCO3 to energy metabolism during exercise., Methods: Following a search through five databases, 17 studies were found to meet the inclusion criteria. Meta-analyses of standardized mean differences (SMDs) were performed using a random-effects model to determine the effects of NaHCO3 supplementation on energy metabolism. Subgroup meta-analyses were conducted for the anaerobic-based exercise (assessed by changes in pH, bicarbonate ion [HCO3 - ], base excess [BE] and blood lactate [BLa]) vs. aerobic-based exercise (assessed by changes in oxygen uptake [VO2 ], carbon dioxide production [VCO2 ], partial pressure of oxygen [PO2 ] and partial pressure of carbon dioxide [PCO2 ])., Results: The meta-analysis indicated that NaHCO3 ingestion improves pH (SMD = 1.38, 95% CI: 0.97 to 1.79, P < 0.001; I2 = 69%), HCO3 - (SMD = 1.63, 95% CI: 1.10 to 2.17, P < 0.001; I2 = 80%), BE (SMD = 1.67, 95% CI: 1.16 to 2.19, P < 0.001, I2 = 77%), BLa (SMD = 0.72, 95% CI: 0.34 to 1.11, P < 0.001, I2 = 68%) and PCO2 (SMD = 0.51, 95% CI: 0.13 to 0.90, P = 0.009, I2 = 0%) but there were no differences between VO2 , VCO2 and PO2 compared with the placebo condition., Conclusions: This meta-analysis has found that the anaerobic metabolism system (AnMS), especially the glycolytic but not the oxidative system during exercise is affected by ingestion of NaHCO3 . The ideal way is to ingest it is in a gelatin capsule in the acute mode and to use a dose of 0.3 g•kg- 1 body mass of NaHCO3 90 min before the exercise in which energy is supplied by the glycolytic system.- Published
- 2021
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33. What Is the Relevance in the Passing Action between the Passer and the Receiver in Soccer? Study of Elite Soccer in La Liga.
- Author
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Cordón-Carmona A, García-Aliaga A, Marquina M, Calvo JL, Mon-López D, and Refoyo Roman I
- Subjects
- Achievement, Probability, Athletic Performance, Soccer
- Abstract
Soccer is a high-complexity sport in which 22 players interact simultaneously in a common space. The ball-holder interacts with their teammates by passing actions, establishing a unique communication among them in the development of the game in its offensive phase. The main aim of the present study was to analyze the pass action according to the trajectory of the ball receiver and the space for receiving the ball in terms of success at the end of play. Twenty La Liga 2018/2019 matches of two elite teams were analyzed. A system of notational analysis was used to create 11 categories based on context, timing and pass analysis. The data were analyzed using chi-squared analysis. The results showed that the main performance indicators were the efficiency of the pass, the zone of the field, the trajectory of the receiver and the reception space of the ball, which presented a moderate association with the end of play ( p < 0.001). We concluded that receiving the ball on approach and in separation increased the probability of success by 5% and 7%, respectively, and a diagonal run increased the probability by 7%. Moreover, the combined analysis of these variables would improve the team performance.
- Published
- 2020
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34. Development and Validation of a Well-Being Measure on Young Basketball Players: The NFAPW Scale.
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Calvo JL, García-González J, Flo García M, and Mon-López D
- Subjects
- Adolescent, Female, Humans, Male, Reproducibility of Results, Young Adult, Athletes psychology, Basketball psychology, Psychometrics statistics & numerical data, Surveys and Questionnaires standards
- Abstract
Basketball is a sport in which, beyond the physical and technical skills, the psychological aspects are a decisive factor and could negatively affect the well-being of the player. The present study analyzes how 11 items belonging to two stakeholders (coach and player) could negatively affect the well-being of the athlete. A sample of elite young basketball players ( n = 121) consisting of 55 males and 66 females, ranging in age from 16 to 23 ( M = 20.12 ± 1.71), completed the Negative Factors Affecting Players' Well-being (NFAPW) Questionnaire. This questionnaire was designed ad hoc and demonstrated good psychometric properties that confirmed that it is a valid and reliable instrument to measure how those factors negatively affect their well-being. The results showed that females have a greater perception of the factors that negatively affect their well-being, especially those related to the actions of the coach. However, no differences were found regarding the experience. Although this research provides an initial tool for measuring the well-being of the player during competition, future studies are encouraged to provide guidance to the coaches and players in dealing with the psychological variables in a better way.
- Published
- 2020
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35. The relationship between the relative age effect and performance among athletes in World Handball Championships.
- Author
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Rubia A, Bjørndal CT, Sánchez-Molina J, Yagüe JM, Calvo JL, and Maroto-Izquierdo S
- Subjects
- Adolescent, Athletes, Female, Humans, Male, Statistics, Nonparametric, Age Factors, Athletic Performance
- Abstract
This study examines the relative age effect (RAE) and its impact on the performance of elite male (n = 3,358) and female (n = 3,273) handball players in the U-19 (n = 2,188), U-21 (n = 2,031), and senior (n = 2,412) categories of the 2013/2014, 2015/2016 and 2017/2018 World Handball Championships organised by the International Handball Federation (IHF). The distribution of the players´ birth dates (by quartile: Q, and semester: S) by categories, handball playing positions, and membership of continental federations, were analysed for each of the three two-year periods in which the World Handball Championships took place. Differences between the observed and expected birth dates were tested using a chi-square goodness of fit test, and subsequent calculations were tested using odds ratios. The Spearman's correlation coefficient was applied to test the correlation between the relative age of the athletes and (their) performance parameters. The results revealed a prevalence of the RAE on both male and female in the U-19 and U-21 categories (p<0.001), and a stabilisation of the RAE throughout the period analysed (2013/14-2017/2018). The RAE was not found in the female senior category (2013, 2015 and 2017) or male senior category (2013) (p>0.05). All playing positions were impacted by the RAE (p<0.001), and especially strong effect sizes were noted for male pivots (Vc = 0.66/0.68/0.60) and female center backs (Vc = 0.71/0.65/0.71). In our analysis of the handball continental federations, the RAE was found in all regions, except Oceania (p>0.05). The RAE also affected athlete performance: the oldest athletes played more minutes and achieved better performances. The RAE was associated with the final placement of the teams in each championship category, except in the male youth category. The findings clearly demonstrated that the RAE has a significant impact on the selection of male and female athletes to international competitions and the performance of both men and women in international handball. The potential implications of these findings for policy and practice are discussed., Competing Interests: The authors have declared that no competing interests exist.
- Published
- 2020
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36. Pistol and Rifle Performance: Gender and Relative Age Effect Analysis.
- Author
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Mon-López D, Tejero-González CM, de la Rubia Riaza A, and Calvo JL
- Subjects
- Adolescent, Adult, Athletes, Female, Humans, Male, Young Adult, Age Factors, Athletic Performance, Firearms, Sex Factors, Sports
- Abstract
Background: The sport overrepresentation of early-born athletes within a selection year is called relative age effect (RAE). Moreover, gender performance differences depend on the sport. The main objectives of the study were to compare performances between gender and RAE in precision shooting events., Method: The results of 704 shooters who participated in the most recent World Shooting Championship were compared. Performance was analysed by event (rifle and pistol), gender and category (junior and senior), together with RAE and six ranges of ranking positions., Results: The results of the study indicated that men scored higher than women in pistol events and that no performance differences were found in rifle events when the whole group was compared. According to the birth trimester, no significant differences were found in the participant's distribution, nor in performance in any case., Conclusions: The main conclusions of the study are: (1) the men's pistol performance is better than the women's even though RAE is not associated to the shooting score in any case; (2) men and women performed equally in the general analysis, but their performances were different depending on category and event with no RAE influence.
- Published
- 2020
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37. Prevalence of Anxiety and Depression in Prostate Cancer Patients and Their Spouses: An Unaddressed Reality.
- Author
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Sánchez Sánchez E, González Baena AC, González Cáliz C, Caballero Paredes F, Moyano Calvo JL, and Castiñeiras Fernández J
- Abstract
Objectives: To estimate the prevalence of unsuspected anxiety or depression in prostate cancer patients and their spouses, as well as factors involved in its onset. Materials and Methods . A prospective study of 184 patients and 137 spouses evaluated in our hospital during 2019 using the Memorial Anxiety Scale for Prostate Cancer (MAX-PC), Hospital Anxiety and Depression Scale (HADS) and Patient Health Questionnaire depression module (PHQ-9). This study provides an internal validity assessment of the scales and their correlation (alpha and rho coefficients; index r ). The contributions of age, education level, months after diagnosis, pain, prostate-specific antigen (PSA) level, stage of the disease and treatment performed to the positivity of the questionnaires were studied using the Wilcoxon-Mann-Whitney and chi-square tests., Results: The prevalence of anxiety was 10.9% (MAX-PC) and 28.3% (MAX-PC-PSA). The HADS-A questionnaire indicated pathology in 14.1% of the patients and 16.05% of the spouses. Depression was detected in 7% (HADS-D) and 9.2% (PHQ-9) of patients as well as in 8.8% (HADS-D) and 16.05% (PHQ-9) of their spouses. The greatest concordance between men and women was with the PHQ-9 (Spearman's rho: 0.78; p = 0.01). Education level is significantly related to the presence of anxiety and depression, regardless of the questionnaire applied. The probability of detecting pathology in the MAX-PC varied from 6% in patients with elementary education to 23.5% in university students ( p = 0.04). The greatest differences were detected when applying the PHQ-9 to patients (4% pathological, elementary education vs. 35.3% pathological, university education). Our study confirms the lack of a relationship between rates of anxiety and depression and factors such as PSA level, age of the patient and number of comorbidities., Conclusion: There is a high prevalence of unsuspected anxiety and depression in patients with prostate cancer and their wives. Education level correlates with such prevalence., Competing Interests: The authors declare no conflicts of interest regarding the publication of this paper., (Copyright © 2020 Ernesto Sánchez Sánchez et al.)
- Published
- 2020
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38. Improvement of the Concrete Permeability by Using Hydrophilic Blended Additive.
- Author
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García Calvo JL, Sánchez Moreno M, Carballosa P, Pedrosa F, and Tavares F
- Abstract
Crystalline hydrophilic additives are increasingly used as efficient methods for reducing water permeability in concrete. Their effectiveness in hindering water penetration has been proven in different cementitious materials, although scarce information has been reported concerning their action mechanism. In the present work, the efficacy of a hydrophilic blended crystalline mix (Krystaline Add1) as a water-reducing additive has been confirmed. Furthermore, an extended study about how the presence of the additive influences both the fresh state and the hardened state properties is presented. Finally, characterization techniques such as Mercury Intrusion Porosimetry (MIP), X-ray Powder Diffraction (XRD) and Back-Scattered Scanning Electron Microscopy (BSEM) with Energy Dispersive X-ray analysis (EDAX) have been used for deducing the mechanism of the additive. No significant deleterious influence on the concrete properties due to the addition of the additive has been detected. In fact, the additive seems to have provided a positive influence on the concrete given that a slight reduction in the w/c ratio for similar consistency has been detected, with the subsequent improvement of the compressive strength values. Its effectiveness as a water permeability reducing additive has shown encouraging results having reduced the water permeability by approximately 50% during testing. The action mechanism of the studied additive seems to be related to hydration reactions in the presence of water, producing new solid amorphous phases in the concrete bulk.
- Published
- 2019
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39. Adaptive fuzzy modeling of the hypnotic process in anesthesia.
- Author
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Marrero A, Méndez JA, Reboso JA, Martín I, and Calvo JL
- Subjects
- Algorithms, Anesthesiology, Artificial Intelligence, Computer Simulation, Female, Fuzzy Logic, Humans, Male, Models, Statistical, Neural Networks, Computer, Operating Rooms, Propofol administration & dosage, Software, Time Factors, Anesthesia methods, Anesthetics administration & dosage, Hypnosis, Anesthetic, Hypnotics and Sedatives administration & dosage
- Abstract
This paper addresses the problem of patient model synthesis in anesthesia. Recent advanced drug infusion mechanisms use a patient model to establish the proper drug dose. However, due to the inherent complexity and variability of the patient dynamics, difficulty obtaining a good model is high. In this paper, a method based on fuzzy logic and genetic algorithms is proposed as an alternative to standard compartmental models. The model uses a Mamdani type fuzzy inference system developed in a two-step procedure. First, an offline model is obtained using information from real patients. Then, an adaptive strategy that uses genetic algorithms is implemented. The validation of the modeling technique was done using real data obtained from real patients in the operating room. Results show that the proposed method based on artificial intelligence appears to be an improved alternative to existing compartmental methodologies.
- Published
- 2017
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40. Influence of Implant Design (Cylindrical and Conical) in the Load Transfer Surrounding Long (13mm) and Short (7mm) Length Implants: A Photoelastic Analysis.
- Author
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Gehrke SA, Frugis VL, Shibli JA, Fernandez MP, Sánchez de Val JE, Girardo JL, Taschieri S, and Corbella S
- Abstract
Purpose: This study compared the influence of implant design (cylindrical and conical) in the load transfer on bone surrounding 13mm and 7mm length implants under simulated occlusal loading, using photoelastic analysis., Method: Dental implants of 4mm diameter were divided into four groups, which varied in length and design: Group 1- standard (13 mm) cylindrical implant; Group 2 - standard conical implant; Group 3 - short (7 mm) cylindrical implant, and Group 4 - short conical implant. After the inclusion of the implant models in a photoelastic resin, they were subjected to a static load of 100 N. The lengths of the fringes that were generated were measured in three portions since the implants body: crestal, central and apical portion, parallel to the implant long axis. Furthermore, the entire extension area of dissipation of force was measured. Data were analyzed by one-way ANOVA (α = 0.05)., Results: Lower stress was observed at the crestal bone in groups 2 and 4, while the stress levels in groups 1 and 3 were higher with significant differences compared to the other groups (p<0.05)., Conclusion: The total amount of stress transmitted to the bone was not affected by implant length under axial loading condition, but changed in relation to the implant design with respect to the concentration of the fringes, which corresponded to the load distribution, with even more dissipation by conical implants.
- Published
- 2016
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41. Implants placed in the nasopalatine canal to rehabilitate severely atrophic maxillae: a retrospective study with long follow-up.
- Author
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Peñarrocha D, Candel E, Guirado JL, Canullo L, and Peñarrocha M
- Subjects
- Adult, Aged, Atrophy, Dental Prosthesis, Implant-Supported, Female, Follow-Up Studies, Humans, Jaw, Edentulous rehabilitation, Jaw, Edentulous surgery, Longitudinal Studies, Male, Maxilla pathology, Middle Aged, Palate innervation, Patient Satisfaction, Pterygopalatine Fossa surgery, Retrospective Studies, Sensory Receptor Cells physiology, Sensory Thresholds physiology, Survival Analysis, Tomography, X-Ray Computed methods, Touch physiology, Treatment Outcome, Visual Analog Scale, Zygoma surgery, Dental Implantation, Endosseous methods, Dental Implants, Maxilla surgery, Palate surgery
- Abstract
To assess the survival rate of implants placed in the nasopalatine canal for the rehabilitation of patients with atrophic maxillae and the level of satisfaction of these patients. A retrospective study was performed between 2000 and 2009 of patients with severe atrophy of edentulous maxillae (Cawood and Howell's class V) rehabilitated with implant-supported prostheses with 1 implant placed in the nasopalatine canal. A preoperative computed tomography scan was obtained of all patients and all surgeries were performed by the same surgeon. The following parameters were assessed: neurosensory status of the anterior palate (using the pointed/blunt discrimination method); implant success rate according to criteria described by Albrektsson et al; patient satisfaction with the prosthetic treatment (using visual analogue scales). Thirteen patients with a mean age of 54.8 years were treated, 5 men and 8 women. Seventy-eight implants were placed: 13 in the nasopalatine canal, 6 in the zygomatic bone, 12 in the pterygomaxillary region, 2 in the frontomaxillary buttress and 45 in other locations. Six patients reported a slight decrease in sensitivity in the anterior palate after surgery, which disappeared in all cases within a few weeks. Two early failures (before prosthetic loading) and no late failures (after prosthetic loading) of nasopalatine implants were recorded, yielding a success rate for these implants of 84.6% after a mean follow-up of 70 months (range 24 to 132 months. High patient satisfaction with the prosthetic restoration was generally achieved in terms of comfort, stability, function, esthetics, and ease of cleaning. Residual bone is associated with the nasopalatine canal, even in patients with severe maxillary atrophy. This canal may be considered a possible location for an anterior implant when rehabilitating atrophic patients using implant-supported prostheses.
- Published
- 2014
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42. In vitro activity of colistin against Stenotrophomonas maltophilia.
- Author
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Rodríguez CH, Nastro M, Calvo JL, Fariña ME, Dabos L, and Famiglietti A
- Abstract
Colistin is one of the few antimicrobials that retains activity against multidrug-resistant Gram-negative bacteria. However, the emergence of colistin resistance has been described recently. The aims of this study were to determine the activity of colistin against isolates of Stenotrophomonas maltophilia. In total, 641 S. maltophilia clinical isolates were obtained from single patients admitted to a university hospital in Buenos Aires city, Argentina, between the years 1996 and 2013. Susceptibility to colistin was determined by the agar dilution method. An increase in colistin resistance from 8% in 1996 to 45% in 2013 was observed, which correlated with a marked increase in colistin consumption of 11.4-fold during the same period., (Copyright © 2014 International Society for Chemotherapy of Infection and Cancer. Published by Elsevier Ltd. All rights reserved.)
- Published
- 2014
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43. Missing data imputation of solar radiation data under different atmospheric conditions.
- Author
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Turrado CC, López Mdel C, Lasheras FS, Gómez BA, Rollé JL, and Juez FJ
- Subjects
- Computer Simulation, Data Interpretation, Statistical, Multivariate Analysis, Radiation Dosage, Reproducibility of Results, Sensitivity and Specificity, Artifacts, Atmosphere analysis, Models, Statistical, Radiometry methods, Sample Size, Solar Energy statistics & numerical data
- Abstract
Global solar broadband irradiance on a planar surface is measured at weather stations by pyranometers. In the case of the present research, solar radiation values from nine meteorological stations of the MeteoGalicia real-time observational network, captured and stored every ten minutes, are considered. In this kind of record, the lack of data and/or the presence of wrong values adversely affects any time series study. Consequently, when this occurs, a data imputation process must be performed in order to replace missing data with estimated values. This paper aims to evaluate the multivariate imputation of ten-minute scale data by means of the chained equations method (MICE). This method allows the network itself to impute the missing or wrong data of a solar radiation sensor, by using either all or just a group of the measurements of the remaining sensors. Very good results have been obtained with the MICE method in comparison with other methods employed in this field such as Inverse Distance Weighting (IDW) and Multiple Linear Regression (MLR). The average RMSE value of the predictions for the MICE algorithm was 13.37% while that for the MLR it was 28.19%, and 31.68% for the IDW.
- Published
- 2014
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44. Behavior of endosseous one-piece yttrium stabilized zirconia dental implants placed in posterior areas.
- Author
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Borgonovo AE, Vavassori V, Censi R, Calvo JL, and Re D
- Subjects
- Adult, Aged, Alveolar Bone Loss etiology, Crowns, Dental Implantation, Endosseous adverse effects, Dental Plaque Index, Dental Prosthesis, Implant-Supported, Dental Restoration Failure, Equipment Failure, Female, Gingival Hemorrhage etiology, Humans, Male, Mandible surgery, Materials Testing, Maxilla surgery, Middle Aged, Molar, Osseointegration, Periodontal Pocket, Dental Alloys, Dental Implantation, Endosseous methods, Dental Implants, Single-Tooth, Dental Prosthesis Design, Yttrium, Zirconium
- Abstract
Aim: Considering that zirconia dental implants are indicated for the rehabilitation of highly esthetic areas, the aim of this work was to investigate the functional behavior of these implants positioned in the posterior regions of the jaws., Methods: The study included 6 patients with 14 zirconia implants positioned in posterior (molar) areas of the jaws. Immediately after surgery, all implants were restored with temporary crowns or bridges. Clinical radiographic evaluation was performed 6 months after surgery and subsequently every year. At these follow-up visits, periodontal indexes were recorded and peri-apical radiographs were taken in order to calculate marginal bone loss., Results: The success and survival rates were 100%. The modified bleeding index (mBI) and modified plaque index (mPLI) were respectively equal to 0.57±0.51 and 0.29 ± 0.47 at 4-year follow-up. The overall mean probing depth of the implants was 3.13±0.87 mm. The mean marginal bone loss was +0.665 mm 4 years after surgery., Conclusion: Considering the limits of our study, as regards to the data concerning peri-implant tissue health, marginal bone loss and survival and success rates of zirconium implants placed in posterior areas, we can conclude that the data reported are very encouraging although the number of implants observed is still limited.
- Published
- 2013
45. Study of the Microstructure Evolution of Low-pH Cements Based on Ordinary Portland Cement (OPC) by Mid- and Near-Infrared Spectroscopy, and Their Influence on Corrosion of Steel Reinforcement.
- Author
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García Calvo JL, Sánchez Moreno M, Alonso Alonso MC, Hidalgo López A, and García Olmo J
- Abstract
Low-pH cements are designed to be used in underground repositories for high level waste. When they are based on Ordinary Portland Cements (OPC), high mineral admixture contents must be used which significantly modify their microstructure properties and performance. This paper evaluates the microstructure evolution of low-pH cement pastes based on OPC plus silica fume and/or fly ashes, using Mid-Infrared and Near-Infrared spectroscopy to detect cement pastes mainly composed of high polymerized C-A-S-H gels with low C/S ratios. In addition, the lower pore solution pH of these special cementitious materials have been monitored with embedded metallic sensors. Besides, as the use of reinforced concrete can be required in underground repositories, the influence of low-pH cementitious materials on steel reinforcement corrosion was analysed. Due to their lower pore solution pH and their different pore solution chemical composition a clear influence on steel reinforcement corrosion was detected.
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- 2013
- Full Text
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46. Metallic ions released from stainless steel, nickel-free, and titanium orthodontic alloys: toxicity and DNA damage.
- Author
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Ortiz AJ, Fernández E, Vicente A, Calvo JL, and Ortiz C
- Subjects
- Cell Line, Cell Survival drug effects, Chromium Alloys toxicity, Comet Assay, Culture Media chemistry, DNA Damage, Humans, Ions analysis, Mass Spectrometry methods, Stainless Steel toxicity, Tetrazolium Salts, Thiazoles, Titanium toxicity, Dental Alloys toxicity, Fibroblasts drug effects, Metals analysis, Orthodontic Brackets adverse effects
- Abstract
Introduction: The aims of this study were to determine the amounts of metallic ions that stainless steel, nickel-free, and titanium alloys release to a culture medium, and to evaluate the cellular viability and DNA damage of cultivated human fibroblasts with those mediums., Methods: The metals were extracted from 10 samples (each consisting of 4 buccal tubes and 20 brackets) of the 3 orthodontic alloys that were submerged for 30 days in minimum essential medium. Next, the determination of metals was performed by using inductively coupled plasma mass spectrometry, cellular viability was assessed by using the tetrazolium reduction assay (MTT assay) (3-[4,5-dimethylthiazol-2-yl]-2, 5-diphenyltetrazolium bromide), and DNA damage was determined with the Comet assay. The metals measured in all the samples were Ti(47), Cr(52), Mn(55), Co(59), Ni(60), Mo(92), Fe(56), Cu(63), Zn(66), As(75), Se(78), Cd(111), and Pb(208)., Results: The cellular viability of the cultured fibroblasts incubated for 7 days with minimum essential medium, with the stainless steel alloy submerged, was close to 0%. Moreover, high concentrations of titanium, chromium, manganese, cobalt, nickel, molybdenum, iron, copper, and zinc were detected. The nickel-free alloy released lower amounts of ions to the medium. The greatest damage in the cellular DNA, measured as the olive moment, was also produced by the stainless steel alloy followed by the nickel-free alloy. Conversely, the titanium alloy had an increased cellular viability and did not damage the cellular DNA, as compared with the control values., Conclusions: The titanium brackets and tubes are the most biocompatible of the 3 alloys studied., (Copyright © 2011 American Association of Orthodontists. Published by Mosby, Inc. All rights reserved.)
- Published
- 2011
- Full Text
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47. Microleakage in Class V composite and compomer restorations following exposure to a colutory prescribed for the treatment of xerostomy.
- Author
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Vicente A, Ortiz AJ, Parra PL, Calvo JL, and Chiva F
- Subjects
- Animals, Cattle, Compomers, Composite Resins, Dental Bonding methods, Dental Leakage etiology, Dental Restoration, Permanent adverse effects, Dental Restoration, Permanent classification, Excipients therapeutic use, Materials Testing, Random Allocation, Saliva metabolism, Stimulation, Chemical, Acid Etching, Dental methods, Dental Leakage prevention & control, Dental Restoration, Permanent methods, Excipients pharmacology, Xerostomia drug therapy
- Abstract
The purpose of this study was to evaluate the effect of a colutory (XeroLacer, Lacer), prescribed for the treatment of xerostomy, on microleakage into class V cavities filled with a composite (Z100, 3M ESPE) or a compomer (F2000, 3M ESPE) in combination with either a two-step etch-and-rinse adhesive (Adper Scotchbond 1XT, 3M ESPE) or a self-etching adhesive (Adper Prompt L-Pop, 3M ESPE). Class V cavities were made in the buccal surfaces of 80 bovine lower incisors which were randomly divided into four groups (n = 20): (1) Adper Scotchbond 1XT/Z100; (2) Adper Scotchbond 1XT/F2000; (3) Adper Prompt L-Pop/Z100; and (4) Adper PLP/F2000. All specimens were immersed in distilled water for 3 months and ten specimens of each group were immersed and vibrated in XeroLacer for 2 min three times a day. The specimens were dyed with 1% methylene blue for 24 h. Longitudinal sections were made and percentages of microleakage were determined by image analysis. In distilled water, microleakage with the Adper Scotchbond 1XT/F2000 combination was significantly greater than with Adper Scotchbond 1XT/Z100 or Adper Prompt L-Pop/Z100, and microleakage with Adper Prompt L-Pop/F2000 was significantly greater than that with Adper Prompt L-Pop/Z100. No significant differences were detected among the groups exposed to XeroLacer. However microleakage was significantly less with Adper Scotchbond 1XT/F2000 after exposure to XeroLacer than after immersion in distilled water only. In conclusion, exposure to XeroLacer decreased microleakage into cavities restored with the compomer.
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- 2011
- Full Text
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48. A randomized clinical evaluation of ultrasound bone surgery versus traditional rotary instruments in lower third molar extraction.
- Author
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Barone A, Marconcini S, Giacomelli L, Rispoli L, Calvo JL, and Covani U
- Subjects
- Adult, Edema prevention & control, Female, Humans, Male, Mandible, Middle Aged, Pain, Postoperative prevention & control, Trismus prevention & control, Young Adult, Molar, Third surgery, Tooth Extraction instrumentation, Ultrasonic Therapy
- Abstract
Purpose: The purpose of this study was to investigate and compare, in a randomized and controlled clinical trial, the use of ultrasound bone surgery devices and the use of rotary instruments in lower third molar extractions., Materials and Methods: We selected 26 patients (12 women and 14 men) for this study; the mean age was 31.2 years (range, 24-45 years). A randomized clinical trial was planned. Patients in the control group received treatment with the conventional rotary instruments; patients in the test group received treatment with the ultrasound bone surgery tools. Twenty-six third molars were allocated to the test and control groups according to a computer-generated randomization list. All the surgical procedures were performed by the same surgeon. Pain, trismus, cheek swelling, and number of analgesics taken were evaluated at baseline (before surgery) and at the first-, third-, fifth-, and seventh-day visits., Results: Pain levels (evaluated on a visual analog scale) were higher in the control group when compared with the ultrasonic group; however, no statistically significant differences were found. On the contrary, the number of analgesics taken in the test group was significantly lower when compared with the control group. The occurrence of trismus was significantly higher in the control group when compared with the test group. The clinical values of cheek swelling were higher in the rotary group when compared with the ultrasound group at the fifth-day visit., Conclusion: This study showed that the use of ultrasound bone surgery for third molar extraction significantly reduced the occurrence of postsurgical trismus, the occurrence of swelling, and the number of analgesics taken after surgery., (Copyright 2010 American Association of Oral and Maxillofacial Surgeons. Published by Elsevier Inc. All rights reserved.)
- Published
- 2010
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49. Comparison of two-step transient evoked otoacoustic emissions (TEOAE) and automated auditory brainstem response (AABR) for universal newborn hearing screening programs.
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Benito-Orejas JI, Ramírez B, Morais D, Almaraz A, and Fernández-Calvo JL
- Subjects
- Evoked Potentials, Auditory, Brain Stem, Humans, Infant, Newborn, Otoacoustic Emissions, Spontaneous, Audiometry, Evoked Response economics, Hearing Disorders diagnosis, Hearing Tests economics, Neonatal Screening economics
- Abstract
Objective: Both transitory auditory otoemissions (TEOAE) and automated auditory brainstem responses (AABR) are considered adequate methods for universal hearing screening. The goal of this study was to compare the results obtained with each device, applying the same screening procedure., Materials and Methods: From 2001 to 2003, all the newborns in our health area (2454 infants) were evaluated with TEOAE (ILO92, otodynamics) and all those born from 2004 to 2006 (3117) were evaluated with AABR (AccuScreen, Fischer-Zoth). The population studied included all well newborns and those admitted to neonatal intensive care units (NICU). The first screening was normally undertaken with well babies during the first 48h of life, before hospital discharge. Infants referred from this first step underwent a second screening after hospital discharge, before they were a month old., Results: The results from each study group were compared and analyzed for significant differences. TEOAE screening yielded 10.2% fail results from the first screening step; AABR gave 2.6%. In the second screening step, 2% of the newborns screened with TEOAE were referred, whereas 0.32% of those screened with AABR were referred. These differences are statistically significant., Conclusions: Although AABR screening tests involve a slightly higher cost in time and money than TEOAE, the results obtained compensate this difference. AABR gives fewer false positives and a lower referral rate; the percent of infants lost during follow-up is consequently smaller. Therefore, in our environment, universal newborn auditory screening with AABR is more effective than that with TEOAE.
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- 2008
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50. [Results of applying a universal protocol for early detection of hypoacusia in newborn infants for 42 months].
- Author
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Benito Orejas JI, Ramírez Cano B, Morais Pérez D, Fernández-Calvo JL, and Almaraz Gómez A
- Subjects
- Child, Preschool, Clinical Protocols, Early Diagnosis, Humans, Infant, Infant, Newborn, Hearing Loss diagnosis, Neonatal Screening standards
- Abstract
Introduction and Objectives: We present the results from applying over a period of three and a half years a universal newborn hearing screening programme aimed at the early detection of hearing loss. Our goal has been to diagnose and treat all unilateral or bilateral permanent hearing losses > or =40 dB nHL., Methods: The detection strategy consists of two screening phases with transient evoked otoacoustic emissions (TEOAE) and one diagnostic phase with auditory brainstem responses (ABR). ABR tests were performed on newborns "not passing" the TEOAE screening, as well as those with risk factors for hearing loss., Results: Of 4568 live newborns (NB), we evaluated 97.6 %. Of these, 3.7 % presented risk factors for hearing loss. The first TEOAE was "passed" in both ears by 91.7 % of the newborns; after the second TEOAE, only 1.3 % of cases were referred to the diagnostic phase. We detected 2.7/1000 cases of severe/profound bilateral hearing loss., Conclusions: This programme was designed for early application of appropriate treatment. Although it is possible to reach the objectives of screening and diagnosis of newborn hearing loss, the intervention stage is much more complicated. This is because intervention depends on different disciplines that must act in co-ordination, as well as multiple environmental and family factors that are difficult to control. Therapeutic effectiveness still remains our challenge and a motive for discussion in fully justifying early congenital hearing loss screening programmes.
- Published
- 2008
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