1,125 results on '"Qiao, Jin"'
Search Results
202. Anthracnose of Tribulus terrestris Caused by Colletotrichum truncatum in Northeast China
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Yi Ming Guan, Hai -Tao Cheng, Lin Lin Zhang, Zheng Bo Liu, Xiao Xi Pan, Shu Na Zhang, Qiao Jin, Yue Zhang, Ning Liu, Ya Yu Zhang, and Qiu-Xia Wang
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Plant Science ,Agronomy and Crop Science - Abstract
Tribulus terrestris L. is an annual herbaceous medicinal plant of Zygophyllaceae, which is cultivated commercially in China. Subrotund or irregular gray, sunken, necrotic spots ranging from 2 to 9 mm were observed on diseased leaves of T. terrestris landrace in Fushun County, Liaoning Province of northeast China in July 2021, with more than 32% of the plants being infected in a 18-ha field. The symptoms first appeared on older leaves and gradually spread to younger leaves. The lesions developed a white center gradually and became perforated; multiple lesions could coalesce (Fig. 1). Ten symptomatic leaves were collected and the diseased tissues were cut into small pieces, immersed in 1% NaOCl for 2 min, rinsed three times with sterile water, and placed on acidified potato dextrose agar (PDA) in Petri dishes at 25°C in darkness. Fifteen suspected Colletotrichum single-spore fungal isolates (JL1 to JL15) with consistent morphological characteristics were obtained, and isolate JL6 was selected for identification and pathogenicity testing. Colonies on PDA were flat with an entire margin, dense and white at first, then became dark gray with numerous black microsclerotia and formed a concentric circular pattern with aging. Conidia were single-celled, sickle-curved with a tapered tip and truncate base, ranging from 16.46 to 20.26 μm in length and 2.81 to 3.96 μm in width (n=100). Setae were dark brown, septate, straight with a slightly acute tip, 75.45 to 135.63×3.19 to 4.95 μm in size. Appressoria were dark brown, round or irregular, mostly in groups. All characteristics were consistent with the descriptions of C. truncatum (Damm et al. 2009). Further confirmation of the identification was determined according to methods described previously (Damm et al. 2009). The rDNA internal transcribed spacer region (OP364400, 585 bp), and actin (OP380867, 290 bp), beta-tubulin (OP380868, 498 bp), chitin synthase 1 (OP380869, 277 bp), glyceraldehyde-3-phosphate dehydrogenase (OP380870, 280 bp), and histone (OP380871, 411 bp) genes were amplified by PCR and sequenced (Carbone and Kohn 1999; Glass and& Donaldson 1995; Guerber et al. 2003; O'Donnell and Cigelnik 1997). BLAST results showed 98-100% similarity at 85-97% coverage compared to the corresponding sequences of the type strain CBS 151.35 (GU227862, GU227960, GU228156, GU228352, GU228254, and GU228058). Phylogenetic analysis combining all loci revealed that the isolate JL6 and the type strains of C. truncatum clustered in one group (Fig. 2). One-year-old healthy seedlings of T. terrestris (cultivar: landrace) were used for pathogenicity test. Suspension (1×105 conidia/mL) of isolate JL6 was sprayed on ten seedlings, and ten seedlings sprayed with sterilized distilled water were used as the control. Three replicates were performed on each treatment. All plants were kept at 28±1°C (12 h photoperiod), and were evaluated after 7 days. The inoculated plants showed lesions on the leaf surface, similar to those in the field, and the control remained symptomless. The pathogen was successfully reisolated and identified using the methods mentioned above. To our knowledge, this is the first report of C. truncatum causing anthracnose on T. terrestris, which will provide valuable information for designing strategies to manage anthracnose on T. terrestris.
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- 2022
203. The relief of hypoxic microenvironment using an O2 self-sufficient fluorinated nanoplatform for enhanced photodynamic eradication of bacterial biofilms
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Fanjin Wang, Jian Ji, Qiao Jin, Lingyun Zou, and Dengfeng Hu
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chemistry.chemical_classification ,Liposome ,Reactive oxygen species ,Chemistry ,medicine.medical_treatment ,Biofilm ,Photodynamic therapy ,Condensed Matter Physics ,Atomic and Molecular Physics, and Optics ,In vitro ,In vivo ,medicine ,Biophysics ,General Materials Science ,Photosensitizer ,Electrical and Electronic Engineering ,Wound healing - Abstract
Hypoxia, an important characteristic of bacterial biofilms, can hinder the generation of reactive oxygen species (ROS) in photodynamic therapy (PDT), leading to reduced therapeutic efficacy of PDT. In order to address this issue, fluorinated liposome was fabricated as an oxygen-sufficient nanoplatform for enhanced photodynamic eradication of bacterial biofilms. The liposomes (denoted as Lip-Ce6-PFH@O2) were prepared by co-encapsulation of O2 carrier perfluorohexane (PFH) and photosensitizer chlorin e6 (Ce6). Lip-Ce6-PFH@O2 could achieve efficient biofilm penetration due to the positively charged surface. The hypoxic microenvironment of biofilms would then be relieved, leading to the generation of more ROS under laser irradiation. Therefore, the bactericidal capability of PDT could be significantly improved because of the co-delivered O2 carrier PFH. Lip-Ce6-PFH@O2 exhibited much better antibiofilm ability than that of Lip-Ce6 both in vitro and in vivo. Meanwhile, Lip-Ce6-PFH@O2 also effectively alleviated inflammation symptoms and accelerated wound healing in the mice model. In general, this study provides a new paradigm to enhance the therapeutic efficacy of PDT for efficient biofilm eradication.
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- 2021
204. EphA2 modulates radiosensitive of hepatocellular carcinoma cells via p38/mitogen-activated protein kinase-mediated signal pathways
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Qiao Jin, Xiangjun Li, and Peiguo Cao
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EphA2 ,Hepatocellular carcinoma cells ,p38/mitogen-activated protein kinase ,Radiosensitivity ,Medicine (General) ,R5-920 - Abstract
This experiment was conducted to investigate the role of EPH receptor A2 (EphA2) in the modulation of radiosensitivity of hepatic cellular cancer (HCC) cells and to determine whether p38/mitogen-activated protein kinase (p38MAPK) signaling mediated EphA2 function in this respect. The protein expressions of EphA2 and phosphorylated p38MAPK were tested in HCC and normal hepatic tissues. In HCC 97H cells, EphA2 was overexpressed and knocked out by transfection with EphA2 expression vector and EphA2-ShRNA, respectively, prior to cell exposure to low-dose irradiation. Significantly upregulated EphA2 and phosphorylated p38MAPK were observed in HCC tissues, compared with those in normal hepatic tissues. Low-dose irradiation (1 Gy) only caused minor damage to HCC 97H cells, as assessed by alterations in cell viability, apoptosis rate, and cell healing capacity (p = 0.072, p = 0.078, and p = 0.069 respectively). However, EphA2 knock-out in HCC 97H cells induced significant reduction in cell viability and cell healing capacity after these cells were subjected to low-dose irradiation. Apoptosis rate underwent dramatic increase (p
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- 2015
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205. A Supramolecular Nitric Oxide Nanodelivery System for Prevention of Tumor Metastasis by Inhibiting Platelet Activation and Aggregation
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Bo Yu, Yongyan Deng, Fan Jia, Youxiang Wang, Qiao Jin, and Jian Ji
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Platelet Aggregation ,General Materials Science ,Nitric Oxide ,Platelet Activation ,Blood Coagulation ,Platelet Aggregation Inhibitors - Abstract
Tumor cell-induced platelet aggregation (TCIPA) is known as a critical step in hematogenous tumor metastasis. The endogenous nitric oxide (NO) plays an important role in anticoagulation, which might have great potential to inhibit TCIPA. Herein, a glutathione-sensitive supramolecular nanocarrier is prepared via host-guest interaction for effective delivery of NO and chemotherapeutic agent gemcitabine (GEM). NO could be effectively released in tumor cells and inhibits platelet activation and aggregation. The inhibition of TCIPA by NO could effectively attenuate the migration and invasion of tumor cells
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- 2022
206. Braiding Lateral Morphotropic Grain Boundaries in Homogenetic Oxides
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Shengru Chen, Qinghua Zhang, Dongke Rong, Yue Xu, Jinfeng Zhang, Fangfang Pei, He Bai, Yan‐Xing Shang, Shan Lin, Qiao Jin, Haitao Hong, Can Wang, Wensheng Yan, Haizhong Guo, Tao Zhu, Lin Gu, Yu Gong, Qian Li, Lingfei Wang, Gang‐Qin Liu, Kui‐juan Jin, and Er‐Jia Guo
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Mechanics of Materials ,Mechanical Engineering ,General Materials Science - Abstract
Interfaces formed by correlated oxides offer a critical avenue for discovering emergent phenomena and quantum states. However, the fabrication of oxide interfaces with variable crystallographic orientations and strain states integrated along a film plane is extremely challenging by conventional layer-by-layer stacking or self-assembling. Here, the creation of morphotropic grain boundaries (GBs) in laterally interconnected cobaltite homostructures is reported. Single-crystalline substrates and suspended ultrathin freestanding membranes provide independent templates for coherent epitaxy and constraint on the growth orientation, resulting in seamless and atomically sharp GBs. Electronic states and magnetic behavior in hybrid structures are laterally modulated and isolated by GBs, enabling artificially engineered functionalities in the planar matrix. This work offers a simple and scalable method for fabricating unprecedented innovative interfaces through controlled synthesis routes as well as providing a platform for exploring potential applications in neuromorphics, solid-state batteries, and catalysis.
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- 2022
207. Identification of
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Qian, Gong, Zhewei, Hu, Qiao, Jin, Yan, Yan, Yan, Liu, Jin, He, Lenan, Zhuang, and Huanan, Wang
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Pulmonary arterial hypertension (PAH) is a pulmonary vascular disease characterized by pulmonary vascular remodeling and right heart enlargement the pathogenesis of PAH is complicated; no biologic-based therapy is available for the treatment of PAH, but recent studies suggest that inflammatory response and abnormal proliferation of pulmonary artery smooth muscle cells are the main pathogenic mechanism, while the role of immune-related long non-coding RNAs (lncRNAs) remains unclear. The aim of this study was to systematically analyze immune-related lncRNAs in PAH. Here, we downloaded a publicly available microarray data from PAH and control patients (GSE113439). A total of 243 up-regulated and 203 down-regulated differentially expressed genes (DEGs) were screened, and immune-related DEGs were further obtained from ImmPort. The immune-related lncRNAs were obtained by co-expression analysis of immune-related mRNAs. Then, immune-related lncRNAs-mRNAs network including 2 lncRNAs and 6 mRNAs was constructed which share regulatory miRNAs and have significant correlation. Among the lncRNA-mRNA pairs, one pair (
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- 2022
208. Targeted delivery of liver X receptor agonist to inhibit neointimal hyperplasia by differentially regulating cell behaviors
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Jian Li, Fan Jia, Zhebin Chen, Jun Lin, Qingbo Lv, Yue Huang, Qiao Jin, Youxiang Wang, Guosheng Fu, and Jian Ji
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Hyperplasia ,Cell Movement ,Neointima ,Liposomes ,Biomedical Engineering ,Human Umbilical Vein Endothelial Cells ,Humans ,Animals ,General Materials Science ,Cells, Cultured ,Rats ,Liver X Receptors ,Cell Proliferation - Abstract
Restenosis induced by neointimal hyperplasia is one of the key reasons limiting the long-term success of cardiovascular interventional therapy. However, it remains a serious challenge to completely overcome restenosis because of the dilemma of simultaneously activating human umbilical vein endothelial cells (HUVECs) and inhibiting human aortic smooth muscle cells (HASMCs). Herein, we developed a targeted nanomedicine encapsulating the liver X receptor (LXR) agonist, T0901317, for differentially regulating the behaviors of HUVECs and HASMCs. The stimulatory effect on HUVEC proliferation/migration and the inhibitory effect on HASMC proliferation/migration were confirmed
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- 2022
209. Resveratrol in the Hypothalamic Paraventricular Nucleus Attenuates Hypertension by Regulation of ROS and Neurotransmitters
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Qi, Jie, primary, Fu, Li-Yan, additional, Liu, Kai-Li, additional, Li, Rui-Juan, additional, Qiao, Jin-An, additional, Yu, Xiao-Jing, additional, Yu, Jia-Yue, additional, Li, Ying, additional, Feng, Zhi-Peng, additional, Yi, Qiu-Yue, additional, Jia, Hong, additional, Gao, Hong-Li, additional, Tan, Hong, additional, and Kang, Yu-Ming, additional
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- 2022
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210. Gravitational Faraday Rotation of gravitational waves by a Kerr black hole
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Li, Zhao, primary, Qiao, Jin, additional, Zhao, Wen, additional, and Er, Xinzhong, additional
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- 2022
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211. Exercise Training Attenuates Hypertension via Suppressing ROS/MAPK/NF-κB/AT-1R Pathway in the Hypothalamic Paraventricular Nucleus
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Qi, Jie, primary, Li, Rui-Juan, additional, Fu, Li-Yan, additional, Liu, Kai-Li, additional, Qiao, Jin-An, additional, Yang, Yu, additional, Yu, Xiao-Jing, additional, Yu, Jia-Yue, additional, Li, Ying, additional, Tan, Hong, additional, and Kang, Yu-Ming, additional
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- 2022
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212. Hypothalamic paraventricular nucleus hydrogen sulfide exerts antihypertensive effects in spontaneously hypertensive rats by attenuating oxidative stress via the Nrf2 pathway
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Xia, Wen-Jie, primary, Yu, Xiao-Jing, additional, Liu, Kai-Li, additional, Wang, Xiao-Min, additional, Yang, Yu, additional, Meng, Tingting, additional, Qiao, Jin-An, additional, Zhang, Nianping, additional, Sun, Yao-Jun, additional, and Kang, Yu-Ming, additional
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- 2022
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213. Raspberry-like gold nanozyme-hybrid liposomes for hypoxia-enhanced biofilm eradication
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Lingyun Zou, Xu Li, Yue Huang, Cong Wang, Yu Fang, Jing Zhao, Qiao Jin, and Jian Ji
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Biomedical Engineering ,Pharmaceutical Science ,General Materials Science ,Bioengineering ,Biotechnology - Published
- 2023
214. Key progresses of MOE key laboratory of macromolecular synthesis and functionalization in 2021
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Quan Wen, Qiuquan Cai, Ping Fu, Dan Chang, Xiaoyi Xu, Tian-Jiao Wen, Guang-Peng Wu, Weipu Zhu, Ling-Shu Wan, Chengjian Zhang, Xing-Hong Zhang, Qiao Jin, Zi-Liang Wu, Chao Gao, Haoke Zhang, Ning Huang, Chang-Zhi Li, and Hanying Li
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General Chemistry - Published
- 2023
215. Development of the Chinese Version of the Parental Child Rearing Scale : A Preliminary Study
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Qiao, Jin and Naoki, Yoneyama
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- 2021
216. Gradient Porous Structure Templated by Breath Figure Method
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Hongye Hao, Bo Yu, Qiao Jin, Yue Huang, Ke-feng Ren, Jian Ji, and Junjie Huang
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Fabrication ,Materials science ,02 engineering and technology ,Surfaces and Interfaces ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,Sample (graphics) ,0104 chemical sciences ,Solvent evaporation ,Electrochemistry ,Immersion (virtual reality) ,General Materials Science ,Relative humidity ,Solvent composition ,Composite material ,0210 nano-technology ,Porosity ,Spectroscopy - Abstract
Surfaces with gradient topography are important in various fields but are difficult to fabricate. Herein, we report a facile and robust way to fabricate a surface with gradient topography of porous structure, in one direction, based on the breath figure (BF) method for the first time. The influencing factors including relative humidity (RH), sample immersion time, and solvent composition, affecting the speed, time, and model of the droplet growth, respectively, were investigated to control gradient BF pores with different ranges of pore sizes. Applying appropriate parameters, gradient BF pores with a diameter difference over 400% were prepared on one sample. The mechanism of gradient duration of solvent evaporation at different regions of a sample for fabricating gradient pores was proposed and experimentally verified with recording optical and thermographic changes of the sample in the BF procedure. This new method provides a novel site for gradient topography fabrication.
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- 2021
217. Construction of the Tetracyclic Framework of Sulfur-Containing Discorhabdin-Type Alkaloids.
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Zhou, Qin, Tao, Yong-Shuai, Qiao, Jin-Bao, and Zhao, Yu-Ming
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- 2023
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218. Effect of drying procedures on the physicochemical properties and antioxidant activities of polysaccharides from Crassostrea gigas.
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Sijie Hu, Guanhua Zhao, Yaxu Zheng, Min Qu, Qiao Jin, Changqing Tong, and Wei Li
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Medicine ,Science - Abstract
Crassostrea gigas polysaccharides (CGP) were obtained by different drying methods: freeze-drying (FD), spray-drying (SD) or rotary evaporation-drying (RED). The physicochemical properties of CGP were evaluated on the basis of polysaccharide content, protein content, color characteristics, FT-IR spectroscopy, differential scanning calorimetry (DSC), and scanning electron microscopy (SEM). Antioxidant activities were researched three different free radicals, including DPPH free radicals, ABTS free radicals and reducing power. The results demonstrated that FDCGP, SDCGP and REDCGP have different physicochemical properties and antioxidant activities. Contrasted with FDCGP and REDCGP, SDCGP exhibited stronger antioxidant abilities. Therefore, considering the polysaccharides appearances and antioxidant activities, the spray drying method is a decent selection for the preparation of such polysaccharides, and it should be selected for application in the food industry.
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- 2017
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219. Cancer-Associated Fibroblast-Targeted Delivery of Captopril to Overcome Penetration Obstacles for Enhanced Pancreatic Cancer Therapy
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Xiaohui Chen, Fan Jia, Yue Huang, Qiao Jin, and Jian Ji
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Biomaterials ,Pancreatic Neoplasms ,Captopril ,Cancer-Associated Fibroblasts ,Biochemistry (medical) ,Liposomes ,Biomedical Engineering ,Humans ,General Chemistry - Abstract
Pancreatic cancer is one of the most stroma-abundant solid cancers. Its desmoplastic nature restricts the penetration of drugs in tumor tissues and is considered as a major challenge for efficient chemotherapy. In the present study, we repurposed the use of captopril to deplete the overexpressed extracellular matrix (ECM) in stroma of pancreatic tumor. Precise delivery of captopril to cancer-associated fibroblasts (CAFs) was achieved using CAFs targeting peptide modified liposomes. The targeted delivery of captopril significantly downregulated the deposition of ECM by blocking the TGF-β1-Smad2 related signaling pathway, which improved the penetration of subsequently administrated liposome-encapsulated chemotherapeutic agent gemcitabine. It proved as a promising solution to break the aforementioned stromal barrier in pancreatic cancer therapy.
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- 2022
220. PM-Search: State-of-the-art Evidence Retriever for Precision Medicine: Algorithm Development and Validation (Preprint)
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Qiao Jin, Chuanqi Tan, Mosha Chen, Ming Yan, Ningyu Zhang, Songfang Huang, and Xiaozhong Liu
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BACKGROUND Under the paradigm of Precision Medicine (PM), patients with the same disease can receive different personalized therapies according to their clinical and genetic features. These therapies are determined by the totality of all available clinical evidence, including results from case reports, clinical trials and systematic reviews. However, it is increasingly difficult for physicians to find such evidence from scientific publications, whose size is growing at an unprecedented pace. OBJECTIVE In this work, we propose the PM-Search system to facilitate the retrieval of clinical literature that contains critical evidence for or against giving specific therapies to certain cancer patients. METHODS The PM-Search system combines a Baseline Retriever that selects document candidates at large scale and an Evidence Re-ranker that finely reorders the candidates based on their evidence quality. The Baseline Retriever uses query expansion and keyword matching with the Elasticsearch retrieval engine, and the Evidence Re-ranker fits pre-trained language models to expert annotations that are derived from an active learning strategy. RESULTS The PM-Search system achieves the best performance in the retrieval of high-quality clinical evidence at the TREC PM Track 2020, outperforming the second-ranking systems by large margins (0.4780 v.s. 0.4238 for standard NDCG@30 and 0.4519 v.s. 0.4193 for exponential NDCG@30). CONCLUSIONS We present PM-Search, a state-of-the-art search engine to assist the practicing of evidence-based PM. PM-Search uses a novel BioBERT-based active learning strategy that models evidence quality and improves the model performance. Our analyses show that evidence quality is a distinct aspect from the general relevance, and specific modeling of evidence quality beyond general relevance is required for a PM search engine.
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- 2022
221. A honeybee stinger-inspired self-interlocking microneedle patch and its application in myocardial infarction treatment
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Yuwen Lu, Tanchen Ren, Hua Zhang, Qiao Jin, Liyin Shen, Mengqi Shan, Xinzhe Zhao, Qichao Chen, Haoli Dai, Lin Yao, Jieqi Xie, Di Ye, Tengxiang Lin, Xiaoqian Hong, Kaicheng Deng, Ting Shen, Jiazhen Pan, Mengyan Jia, Jun Ling, Peng Li, Yue Zhang, Huanan Wang, Lenan Zhuang, Changyou Gao, Jifu Mao, and Yang Zhu
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Microinjections ,Swine ,Biomedical Engineering ,Myocardial Infarction ,General Medicine ,Punctures ,Bees ,Biochemistry ,Biomaterials ,Drug Delivery Systems ,Needles ,Animals ,Molecular Biology ,Biotechnology - Abstract
Weak tissue adhesion remains a major challenge in clinical translation of microneedle patches. Mimicking the structural features of honeybee stingers, stiff polymeric microneedles with unidirectionally backward-facing barbs were fabricated and embedded into various elastomer films to produce self-interlocking microneedle patches. The spirality of the barbing pattern was adjusted to increase interlocking efficiency. In addition, the micro-bleeding caused by microneedle puncturing adhered the porous surface of the patch substrate to the target tissue via coagulation. In the demonstrative application of myocardial infarction treatment, the bioinspired microneedle patches firmly fixed on challenging beating hearts, significantly reduced cardiac wall stress and strain in the infarct, and maintained left ventricular function and morphology. In addition, the microneedle patch was minimally invasively implanted onto beating porcine heart in 10 minutes, free of sutures and adhesives. Therefore, the honeybee stinger-inspired microneedles could provide an adaptive and convenient means to implant patches for various medical applications. STATEMENT OF SIGNIFICANCE: Adhesion between tissue and microneedle patches with smooth microneedles is usually weak. We introduce a novel barbing method of fabricating unidirectionally backward facing barbs with controllable spirality on the microneedles on microneedle patches. The microneedle patches self-interlock on mechanically dynamic beating hearts, similar to honeybee stingers. The micro-bleeding and coagulation on the porous surface provide additional adhesion force. The microneedle patches attenuate left ventricular remodeling via mechanical support and are compatible with minimally invasive implantation.
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- 2022
222. Tuning superconductivity in vanadium nitride films by adjusting strain
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Yuting Zou, Qiao Jin, Yuxin Wang, Kun Jiang, Shanmin Wang, Yangmu Li, Er-Jia Guo, and Zhi Gang Cheng
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- 2022
223. A mitochondria-targeted supramolecular nanoplatform for peroxynitrite-potentiated oxidative therapy of orthotopic hepatoma
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Wenbin Dai, Yongyan Deng, Xiaohui Chen, Yue Huang, Haitao Hu, Qiao Jin, Zhe Tang, and Jian Ji
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Carcinoma, Hepatocellular ,Liver Neoplasms ,Biophysics ,Mice, Nude ,Bioengineering ,Nitric Oxide ,Glutathione ,Mitochondria ,Biomaterials ,Mice ,Oxidative Stress ,Mechanics of Materials ,Peroxynitrous Acid ,Ceramics and Composites ,Animals ,Reactive Oxygen Species - Abstract
Oxidative therapy, which generates reactive oxygen species (ROS) via intracellular enzymatic reactions to achieve tumor ablation, is considered as an emerging approach to cancer treatment. Herein, nitric oxide (NO)-combined oxidative therapy is reported by integrating glutathione (GSH)-sensitive NO donor and pH-sensitive cinnamaldehyde (CA) prodrug into a mitochondria-targeted drug nanocarrier, which is prepared by the host-guest interaction between α-cyclodextrin (α-CD) and polyethylene glycol (PEG). After internalized by cancer cells, CA can be released in acidic endo/lysosome and finally induce ROS generation in mitochondria for oxidative therapy. At the same time, NO can be targeted delivered to mitochondria by a mitochondria-targeting strategy and then realize selective release of NO in mitochondria. NO can deplete intracellular predominant antioxidant GSH, which will enhance oxidative therapy of CA. Furthermore, peroxynitrite (ONOO
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- 2022
224. Fabrication of a nanocomposite film decorated with highly dispersive nanoparticles by following an interface-induced strategy
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Rui Ma, Yue Huang, Jiaqi Huang, Kaixuan Zheng, Shujuan Jiang, Qiao Jin, and Wei Sun
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Silver ,Polymers ,Materials Chemistry ,Metals and Alloys ,Ceramics and Composites ,Nanoparticles ,General Chemistry ,Catalysis ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Anti-Bacterial Agents ,Nanocomposites - Abstract
A polymer nanocomposite film decorated with highly dispersive nanoparticles was prepared by a liquid-liquid interface induced self-assembly method based on a breath figure process. The distribution as well as the orientation preference of the Janus particles within the polymer matrix could be dynamically controlled by adjusting the environmental conditions. Antibacterial and photocatalytic functionality was obtained for the nanocomposite films decorated with silver and titanium dioxide nanoparticles, respectively.
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- 2022
225. Light-Responsive Polyion Complex Micelles with Switchable Surface Charge for Efficient Protein Delivery
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Tongjiang Cai, Haibo Wang, Jian Ji, Yin Wang, and Qiao Jin
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Circular dichroism ,Chromatography ,Polymers and Plastics ,biology ,Chemistry ,Organic Chemistry ,Cationic polymerization ,Photochemistry ,Micelle ,Inorganic Chemistry ,Dynamic light scattering ,Materials Chemistry ,biology.protein ,Zeta potential ,Copolymer ,Surface charge ,Bovine serum albumin - Abstract
In this letter, light-responsive protein-encapsulated polyion complex (PIC) micelles were prepared by self-assembly of cationic block copolymer poly(N,N-dimethyl-N-(2-(methacryloyloxy)ethyl)-N-((2-nitrobenzyl)oxy)-2-oxoethanaminium bromide)-block-poly(carboxybetaine methacrylate) (PDMNBMA-b-PCBMA) and negatively charged bovine serum albumin (BSA). The PIC micelles were well characterized by dynamic light scattering (DLS) and transmission electron microscopy (TEM). From the zeta potential measurement, the increase of the zeta potential of PIC micelles from ∼10 to ∼20 mV was observed when the solution pH decreased from 7.4 to 6.5, which could enhance the intracellular protein delivery efficiency. Moreover, the positively charged PDMNBMA blocks can be transformed to zwitterionic carboxybetaine units under UV irradiation, which could result in the disassembly of the PIC micelles. The release of BSA can therefore be drastically accelerated in the presence of UV irradiation. Meanwhile, the circular dichroism (C...
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- 2022
226. Cardiac patch treatment alleviates ischemic cardiomyopathy correlated with reverting Piezo1/2 expression by unloading left ventricular myocardium
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Yang Zhu, Yuwen Lu, Sibo Jiang, Ting Shen, Chengbin He, Yun Gao, Liyin Shen, Qiao Jin, Yuting Zhao, Hongjie Hu, Jun Ling, Jin He, and Lenan Zhuang
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Pathologically elevated mechanical load promotes the adverse remodeling of left ventricle (LV) post myocardial infarction, which results in the progression from ischemic cardiomyopathy to heart failure. Cardiac patches could attenuate adverse LV remodeling by providing mechanical support to infarcted myocardium and border zone tissue. However, the mechanism of the translation from mechanical effects to favorable therapeutic outcome is still not clear. By transcriptome analysis, we found that the myocardial transcription levels of mechanosensitive ion channel proteins Piezo1 and Piezo2 significantly increased in patients with ischemic cardiomyopathy. In vitro tensile tests with local tissue information revealed a significant decrease in local strain and mechanical load in rat infarct. Cardiac function and geometry were preserved compared to non-treated control. Further, in LV myocardium of the patch-treated group, MI induced expression levels of Piezo1/2 were significantly reverted to the similar levels of the Sham group, indicating that cardiac patch beneficial effects were correlated with suppressing mechanosensitive genes, particularly Piezo1/2. These findings demonstrated the potential of cardiac patches in treating ICM patients with remodeling risks, and could provide guidance for improvement in next generation of patch devices.
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- 2022
227. TLCD Semi-active Control Methodology of Fuzzy Neural Network for Eccentric Buildings.
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Hong-Nan Li, Qiao Jin, Gangbing Song, and Guo-Xin Wang
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- 2005
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228. Tailoring Supramolecular Prodrug Nanoassemblies for Reactive Nitrogen Species-Potentiated Chemotherapy of Liver Cancer
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Qiao Jin, Fan Jia, Dongfang Zhou, Yupeng Wang, Weifeng Liu, Jian Ji, and Yongyan Deng
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Xeroderma pigmentosum ,medicine.medical_treatment ,General Physics and Astronomy ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Nitric oxide ,chemistry.chemical_compound ,Superoxides ,medicine ,Humans ,Prodrugs ,General Materials Science ,Reactive nitrogen species ,Cisplatin ,Chemotherapy ,Superoxide ,Liver Neoplasms ,General Engineering ,Prodrug ,021001 nanoscience & nanotechnology ,medicine.disease ,Reactive Nitrogen Species ,0104 chemical sciences ,chemistry ,Cancer research ,Reactive Oxygen Species ,0210 nano-technology ,Peroxynitrite ,medicine.drug - Abstract
The development of a controllable reactive nitrogen species (RNS) generation system for cancer treatment has remained elusive. Herein, a supramolecular prodrug nanoassemblies (SPNA) strategy that co-delivers a nitric oxide (NO) donor and a superoxide anion (O2•-) inducing chemotherapeutic agent was reported for RNS-potentiated chemotherapy. The mole ratio of platinum(IV) prodrug and NO donor could be precisely tailored in SPNAPt/NO. Platinum(II) and NO would be released intracellularly to produce a highly toxic RNS, peroxynitrite anion (ONOO-). The levels of glutathione reductase (GR) and xeroderma pigmentosum group A (XPA) were down-regulated by ONOO-, thus synergistically decreasing detoxification and blocking DNA damage repair of Pt-based chemotherapy. The RNS-potentiated efficacy of SPNAPt/NO was validated on subcutaneous hepatoma xenograft models and an orthotopic cisplatin-resistant hepatoma model. This co-delivery strategy of NO donor and O2•- inducing chemotherapeutic agents for RNS-mediated therapy provides an insightful direction for cancer treatment.
- Published
- 2021
229. Antimicrobial nanomedicine for ocular bacterial and fungal infection
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Yifan Gao, Yaoyao Chen, Jian Ji, Qiao Jin, Wenjie Fan, Haijie Han, Xiaobo Zhang, Su Li, and Ke Yao
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genetic structures ,medicine.drug_class ,Secondary infection ,Antibiotics ,Pharmaceutical Science ,02 engineering and technology ,030226 pharmacology & pharmacy ,Release time ,Cornea ,03 medical and health sciences ,0302 clinical medicine ,Anti-Infective Agents ,medicine ,Humans ,Prospective Studies ,Pathogen ,Bacteria ,Infection induced ,business.industry ,021001 nanoscience & nanotechnology ,Antimicrobial ,eye diseases ,Anti-Bacterial Agents ,Nanomedicine ,medicine.anatomical_structure ,Mycoses ,Immunology ,sense organs ,0210 nano-technology ,business - Abstract
Ocular infection induced by bacteria and fungi is a major cause of visual impairment and blindness. Topical administration of antibiotics remains the first-line treatment, as effective eradication of pathogens is the core of the anti-infection strategy. Whereas, eye drops lack efficiency and have relatively low bioavailability. Intraocular injection may cause concurrent ocular damage and secondary infection. In addition, antibiotic-based management can be limited by the low sensitivity to multidrug-resistant bacteria. Nanomedicine is proposed as a prospective, effective, and noninvasive platform to mediate ocular delivery and combat pathogen or even resistant strains. Nanomedicine can not only carry antimicrobial agents to fight against pathogens but also directly active microbicidal capability, killing pathogens. More importantly, by modification, nanomedicine can achieve enhanced residence time and release time on the cornea, and easy penetration through corneal tissues into anterior and posterior segments of the eye, thus improving the therapeutic effect for ocular infection. In this review, several categories of antimicrobial nanomedicine are systematically discussed, where the efficiency and possibility of further embellishment and improvement to adapt to clinical use are also investigated. All in all, novel antimicrobial nanomedicine provides potent and prospective ways to manage severe and refractory ocular infections.
- Published
- 2021
230. Evaluation of the Structural Phase Transition in Multiferroic (Bi1−x Prx)(Fe0.95 Mn0.05)O3 Thin Films by A Multi-Technique Approach Including Picosecond Laser Ultrasonics
- Author
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Samuel Raetz, Alexey Lomonosov, Sergey Avanesyan, Nikolay Chigarev, Elton de Lima Savi, Alain Bulou, Nicolas Delorme, Zheng Wen, Qiao Jin, Maju Kuriakose, Anthony Rousseau, Gwenaëlle Vaudel, Pascal Ruello, Di Wu, and Vitalyi Gusev
- Subjects
picosecond laser ultrasonics ,time-domain Brillouin scattering ,non-destructive depth-profiling ,structural phase transition ,multiferroics ,multi-technique approach ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Picosecond laser ultrasonics is an experimental technique for the generation and detection of ultrashort acoustic pulses using ultrafast lasers. In transparent media, it is often referred to as time-domain Brillouin scattering (TDBS). It provides the opportunity to monitor the propagation of nanometers-length acoustic pulses and to determine acoustical, optical, and acousto-optical parameters of the materials. We report on the application of TDBS for evaluating the effect of Praseodymium (Pr) substitution on the elasticity of multiferroic (Bi1−xPrx)(Fe0.95Mn0.05)O3 (BPFMO) thin films. The films were deposited on Si and LaAlO3 (LAO) substrates by a sol-gel method. X-ray diffraction and Raman spectra revealed earlier that a phase transition from rhombohedral to tetragonal structure occurs at about 15% Pr substitution and is accompanied by the maxima of remnant magnetization and polarization. Combining TDBS with optical spectral reflectometry, scanning electron microscopy, and topographic measurements by atomic force microscopy, we found that the structural transition is also characterized by the maximum optical dielectric constant and the minimum longitudinal sound velocity. Our results, together with earlier ones, suggest that BiFeO3-based films and ceramics with compositions near phase boundaries might be promising materials for multifunctional applications.
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- 2019
- Full Text
- View/download PDF
231. Task-State Cortical Motor Network Characteristics by Functional Near-Infrared Spectroscopy in Subacute Stroke Show Hemispheric Dominance
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Yuan, Ziwen, primary, Xu, Weiwei, additional, Bao, Jiameng, additional, Gao, Hui, additional, Li, Wen, additional, Peng, Yu, additional, Wang, Lisha, additional, Zhao, Ye, additional, Song, Siming, additional, Qiao, Jin, additional, and Wang, Gang, additional
- Published
- 2022
- Full Text
- View/download PDF
232. Fuzzy neural network based TLCD semi-active control methodology for irregular buildings.
- Author
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Hong-Nan Li, Qiao Jin, Gangbing Song, and Guo-Xin Wang
- Published
- 2003
- Full Text
- View/download PDF
233. Vibration analysis of a sandwich cylindrical shell in hygrothermal environment
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Limeng Zhu, Qiao Jin, Haicheng Liu, Xiao Hongmei, Jingli Wang, He Tai, Chunwei Zhang, Li Sun, Songlin Guo, Xiaodong Yuan, Yansheng Song, and Limin Dun
- Subjects
Technology ,Materials science ,Physical and theoretical chemistry ,QD450-801 ,Shell (structure) ,Energy Engineering and Power Technology ,Medicine (miscellaneous) ,TP1-1185 ,02 engineering and technology ,Biomaterials ,Exfoliated graphite nano-platelets ,0203 mechanical engineering ,Composite material ,Chemical technology ,Process Chemistry and Technology ,graphene nanoplatelets ,sandwich cylindrical shell ,021001 nanoscience & nanotechnology ,Surfaces, Coatings and Films ,Vibration ,hygrothermal environment ,020303 mechanical engineering & transports ,sinusoidal shear deformation ,vibration ,0210 nano-technology ,Porous medium ,porous materials ,Biotechnology - Abstract
The sandwich structures are three- or multilayered structures such that their mechanical properties are better than each single layer. In the current research, a three-layered cylindrical shell including a functionally graded porous core and two reinforced nanocomposite face sheets resting on the Pasternak foundation is used as model to provide a comprehensive understanding of vibrational behavior of such structures. The core is made of limestone, while the epoxy is utilized as the top and bottom layers’ matrix phase and also it is reinforced by the graphene nanoplatelets (GNPs). The pattern of the GNPs dispersion and the pores distribution play a crucial role at the continuous change of the layers’ properties. The sinusoidal shear deformation shells theory and the Hamilton’s principle are employed to derive the equations of motion for the mentioned cylindrical sandwich shell. Ultimately, the impacts of the model’s geometry, foundation moduli, mode number, and deviatory radius on the vibrational behavior are investigated and discussed. It is revealed that the natural frequency and rotation angle of the sandwich shell are directly related. Moreover, mid-radius to thickness ratio enhancement results in the natural frequency reduction. The results of this study can be helpful for the future investigations in such a broad context. Furthermore, for the pipe factories current study can be effective at their designing procedure.
- Published
- 2021
234. Mixed-charge modification as a robust method to realize the antiviral ability of gold nanoparticles in a high protein environment
- Author
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Yue Huang, Jian Ji, Qiao Jin, and Xu Li
- Subjects
Chemistry ,Ligand ,High protein ,Metal Nanoparticles ,Nanoparticle ,Ligands ,Antiviral Agents ,Combinatorial chemistry ,Protein environment ,In vivo ,Colloidal gold ,Viruses ,Humans ,Nanomedicine ,General Materials Science ,Gold - Abstract
Pandemics caused by viruses have resulted in incalculable losses to human beings, which are exacerbated due to the lack of antiviral drugs. Sulfonic group modified nanomedicine has been proved to possess a broad-spectrum antiviral ability. However, it is very challenging to maintain the antiviral activity in a high protein environment in vivo. To improve the tolerance to the complex biological environment, sulfonic mixed-charge modified gold nanoparticles (MC_AuNPs) were prepared in this research by introducing positively charged ligands into sulfonic ligand modified gold nanoparticles. The MC_AuNPs showed excellent non-fouling ability while retaining comparable antiviral ability to single sulfonic ligand modified gold nanoparticles (MDS_AuNPs). The MC_AuNPs maintained their antiviral ability in 10 mg mL−1 protein solutions, but the MDS_AuNPs completely lost their antiviral capability in 1 mg mL−1 protein medium. The mixed-charge modification strategy provides a practical avenue to maintain the antiviral capability of HSPG mimicking nanoparticles in high protein environments.
- Published
- 2021
235. Circ_ROBO2/miR-149 Axis Promotes the Proliferation and Migration of Human Aortic Smooth Muscle Cells by Activating NF-κB Signaling
- Author
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Chi-Yuan Zhang, Qiao Jin, Liang Li, Guo-Hong Ye, Lu-Ping Jiang, and Dong-Sheng Lin
- Subjects
Gene knockdown ,medicine.diagnostic_test ,Cell growth ,Myocytes, Smooth Muscle ,Becaplermin ,NF-kappa B ,RNA ,RNA, Circular ,Biology ,Flow cytometry ,Cell biology ,MicroRNAs ,Downregulation and upregulation ,Western blot ,Cell Movement ,Apoptosis ,Genetics ,medicine ,Humans ,Gene silencing ,Receptors, Immunologic ,Molecular Biology ,Genetics (clinical) ,Cell Proliferation - Abstract
Atherosclerosis is the leading global cause of mortality. The occurrence of coronary artery disease (CAD) is regulated by a diversity of pathways, including circRNAs. However, the potential mechanisms of circRNAs in CAD remain unclear. Here, qRT-PCR was used to examine the expressions of miR-149 and circ_ROBO2. Their influences on cell proliferation, migration, and apoptosis were measured by CCK-8, transwell, and flow cytometry assays, respectively. The protein levels of p-IκBα and NF-κB p65 were examined using western blot. The molecular interactions were validated using dual luciferase reporter and RNA pull-down assays. The expression patterns of circ_ROBO2 and miR-149 in CAD patients and PDGF-BB-treated human aortic smooth muscle cells (HASMCs) were upregulated and downregulated, respectively. Knockdown of circ_ROBO2 could markedly inhibit the capabilities of proliferation and migration, enhance the apoptotic rate, and suppress NF-κB signaling in PDGF-BB-treated HASMCs. Mechanistically, circ_ROBO2 acted as a sponge of miR-149 to activate TRAF6/NF-κB signaling. Rescue studies demonstrated that neither silencing miR-149 nor activation of NF-κB signaling obviously abolished the biological roles of circ_ROBO2 knockdown in PDGF-BB treated-HASMCs. This discovery elucidated a functional mechanism of circ_ROBO2 in CAD, suggesting that circRNAs serve a vital role in the progression of CAD.
- Published
- 2021
236. Macromolecular Platform with Super-Cation Enhanced Trans-Cornea Infiltration for Noninvasive Nitric Oxide Delivery in Ocular Therapy
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Yu Fang, Maomao Song, Yuan Lei, Fan Jia, Liping Li, Jian Ji, Qiao Jin, and Jiaping Man
- Subjects
Drug ,Polyamino acid ,media_common.quotation_subject ,General Physics and Astronomy ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Nitric oxide ,No donors ,chemistry.chemical_compound ,Cornea ,medicine ,General Materials Science ,media_common ,Chemistry ,General Engineering ,021001 nanoscience & nanotechnology ,medicine.disease ,eye diseases ,0104 chemical sciences ,medicine.anatomical_structure ,Transcytosis ,Drug delivery ,Biophysics ,sense organs ,0210 nano-technology ,Infiltration (medical) - Abstract
The cornea provides important protection for human eyes from invasion of alien substances. However, its blockage on the infiltration of molecules also constitutes a great challenge for noninvasive trans-cornea delivery of drugs. Here we report polyamino acid-based S-nitrosothiols with high cationic charge density as a NO carrier to overcome cornea associated blockage in ophthalmological therapy. Our results demonstrate that the cationic nature of the polymer promoted transcytosis, which greatly enhances the trans-cornea delivery of the NO donor and bypasses cornea barriers on passive drug diffusion. The combination of super cation and glutathione responsiveness synergistically enhanced intraocular delivery of topically administered poly(2-acetamido-N-triethylenetetramine-3-nitrosothiol-3-methylbutanamide)aspartamide, effectively alleviating high intraocular pressure in mice with glaucoma. Such a noninvasive "barrier hopping" approach not only serves as an inspiration in improving the efficiency of trans-cornea drug delivery but also has great potential in overcoming drug transporting barriers in other biomedical applications.
- Published
- 2020
237. A NIR-II emissive polymer AIEgen for imaging-guided photothermal elimination of bacterial infection
- Author
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Yue Huang, Dan Li, Deliang Wang, Xiaohui Chen, Lino Ferreira, M. Cristina L. Martins, Youxiang Wang, Qiao Jin, Dong Wang, Ben Zhong Tang, and Jian Ji
- Subjects
Biomaterials ,Methicillin-Resistant Staphylococcus aureus ,Mice ,Bacteria ,Mechanics of Materials ,Polymers ,Biophysics ,Ceramics and Composites ,Animals ,Bioengineering ,Bacterial Infections ,Phototherapy ,Anti-Bacterial Agents - Abstract
The development of antibiotics resistance has made multidrug-resistant (MDR) bacterial infection one of the most serious global health issues. Photothermal therapy (PTT) is an emerging therapeutic mode which can be applied to bacterial infection without inducing resistance. Moreover, enhanced therapeutic efficacy and less tissue damage can be realized with NIR-II fluorescence imaging (FLI) guided PTT. Herein, a polymeric luminogen with aggregation-induced emission (AIEgens) characteristics, poly(dithieno[3,2-b:2',3'-d]pyrrole-benzo[1,2-c:4,5-c']bis([1,2,5]thiadiazole)) (PDTPTBT), was synthesized and used as a photothermal agent for PTT of bacterial infections. PDTPTBT was encapsulated into liposomes (L-PDTPTBT) for improved water dispersibility. Upon 808 nm NIR irradiation, L-PDTPTBT can eliminate multiple bacteria including the Gram-positive methicillin-resistant Staphylococcus aureus and Enterococcus faecalis, the Gram-negative Escherichia coli and Pseudomonas aeruginosa. Serious damage of bacterial membrane and leakage of cytoplasm is observed after photothermal treatment using L-PDTPTBT. The potential of the formulation has been demonstrated in two infected animal models: (i) a subcutaneous abscess model and (ii) a diabetic skin infection model. In the diabetic skin infection model, the death of mice is largely suppressed and the wounds can heal more quickly with treatment of L-PDTPTBT under NIR irradiation. The excellent photothermal bactericidal ability and low cytotoxicity make L-PDTPTBT potential candidate for treating MDR bacterial infections in the future.
- Published
- 2022
238. Stimuli‐responsive nanoplatforms for antibacterial applications
- Author
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Yue Huang, Lingyun Zou, Jing Wang, Qiao Jin, and Jian Ji
- Subjects
Drug Liberation ,Drug Delivery Systems ,Nanomedicine ,Biomedical Engineering ,Humans ,Nanoparticles ,Medicine (miscellaneous) ,Bioengineering ,Bacterial Infections ,Anti-Bacterial Agents - Abstract
The continuously increasing bacterial resistance has become a big threat to public health worldwide, which makes it urgent to develop innovative antibacterial strategies. Nanotechnology-based drug delivery systems are considered as promising strategies in combating bacterial infections which are expected to improve the therapeutic efficacy and minimize the side effects. Unfortunately, the conventional nanodrug delivery systems always suffer from practical dilemmas, including incomplete and slow drug release, insufficient accumulation in infected sites, and weak biofilm penetration ability. Stimuli-responsive nanoplatforms are hence developed to overcome the disadvantages of conventional nanoparticles. In this review, we provide an extensive review of the recent progress of endogenous and exogenous stimuli-responsive nanoplatforms in the antibacterial area, including planktonic bacteria, intracellular bacteria, and bacterial biofilms. Taking advantage of the specific infected microenvironment (pH, enzyme, redox, and toxin), the mechanisms and strategies of the design of endogenous stimuli-responsive nanoplatforms are discussed, with an emphasis on how to improve the therapeutic efficacy and minimize side effects. How to realize controlled drug delivery using exogenous stimuli-responsive nanoplatforms especially light-responsive nanoparticles for improved antibacterial effects is another topic of this review. We especially highlight photothermal-triggered drug delivery systems by the combination of photothermal agents and thermo-responsive materials. This article is categorized under: Therapeutic Approaches and Drug DiscoveryNanomedicine for Infectious Disease Therapeutic Approaches and Drug DiscoveryEmerging Technologies.
- Published
- 2022
239. The binding of resveratrol to monomer and fibril amyloid beta
- Author
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Ge, Jin-Fang, Qiao, Jin-Ping, Qi, Cong-Cong, Wang, Chen-Wei, and Zhou, Jiang-Ning
- Published
- 2012
- Full Text
- View/download PDF
240. Applying transcranial magnetic stimulation to rehabilitation of poststroke lower extremity function and an improvement: Individual‐target TMS.
- Author
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Qi, Shun, Tian, Meng, Rao, Yang, Sun, Chuanzhu, Li, Xiang, Qiao, Jin, and Huang, Zi‐Gang
- Subjects
TRANSCRANIAL magnetic stimulation ,STROKE ,REHABILITATION ,MOTOR cortex ,CLINICAL neurosciences - Abstract
Stroke is the leading cause of disability globally in need of novel and effective methods of rehabilitation. Intermittent theta burst stimulation (iTBS) has been adopted as a Level B recommendation for lower limb spasticity in guidelines on the therapeutic use of repetitive transcranial magnetic stimulation (rTMS). Nonetheless, the methodological differences and deficits of existing work bring about heterogenous results and therefore limit the universal clinical use of rTMS in lower extremity (LE) rehabilitation. The variation of stimulated targets across motor cortex contributes mainly to these heterogeneities. This narrative review includes studies of rTMS on LE motor function rehabilitation in patients after stroke until now. Some analyses of brain imaging and electromagnetic simulation and quantification through computational modeling were also performed. rTMS appears capable of fostering LE motor rehabilitation after stroke, but the actually stimulated targets are considerably bias making it difficult to confirm effectiveness. The main reason for this phenomenon is probably inaccurate targeting of motor cortical leg representation. An underlying updated method is proposed as Individual‐Target TMS (IT‐TMS) combined with brain imaging. rTMS is a promising validated method for LE function regaining. Future studies should systematically compare the effects of IT‐TMS with traditional rTMS using large samples in random clinical trials. This article is categorized under:Neuroscience > Clinical Neuroscience [ABSTRACT FROM AUTHOR]
- Published
- 2023
- Full Text
- View/download PDF
241. Effect of nano-fillers on conductivity of polyethylene/low melting point metal alloy composites
- Author
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Chen, Gang, Zhang, Xiao-hong, and Qiao, Jin-liang / 乔金樑
- Published
- 2015
- Full Text
- View/download PDF
242. The electrical properties and crystallization of stereocomplex poly(lactic acid) filled with carbon nanotubes
- Author
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Quan, Hui, Zhang, Shi-jun, Qiao, Jin-liang, and Zhang, Li-ying
- Published
- 2012
- Full Text
- View/download PDF
243. Post-Newtonian parameters of ghost-free parity-violating gravities
- Author
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Qiao, Jin, primary, Zhu, Tao, additional, Li, Guoliang, additional, and Zhao, Wen, additional
- Published
- 2022
- Full Text
- View/download PDF
244. Polymer coated nanodiamonds as gemcitabine prodrug with enzymatic sensitivity for pancreatic cancer treatment
- Author
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Huan Li, Jian Ji, Yuzhou Wu, Wanying Ye, Sabyasachi Chakrabortty, Haijie Han, Qiao Jin, and Tanja Weil
- Subjects
Enzyme-sensitive ,Materials science ,Biocompatibility ,endocrine system diseases ,02 engineering and technology ,Self-assembly ,Prodrug ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Controlled release ,Combinatorial chemistry ,0104 chemical sciences ,Nanodiamonds ,Colloidal gold ,Drug delivery ,Zeta potential ,lcsh:TA401-492 ,General Materials Science ,lcsh:Materials of engineering and construction. Mechanics of materials ,Nanocarriers ,Gemcitabine prodrug ,0210 nano-technology ,Nanodiamond - Abstract
The fabrication of Gemcitabine (GEM) prodrug was reported to be an effective method to enhance its pancreatic cancer treatment efficiency. Here, a kind of nanocarbon-based materials, nanodiamond (ND), was selected as the nanocarrier of GEM, owing to its outstanding surface properties and non-cytotoxicity. The polyelectrolytes, polyethyleneimine and polyacrylic acid, were used to self-assemble outside ND surface through electrostatic forces, followed by attachment of polyethylene glycol to address better biocompatibility. GEM was conjugated with an enzyme-sensitive peptide gly-phe-leu-gly to build up the controlled release platform. From characterization results of dynamic laser scattering, zeta potential and transmission electron microscope, the significant improvement of ND stability in physiological condition was proved. Non-cytotoxicity of this functionalized ND carriers and cytotoxicity of the prodrug against BxPC-3 pancreatic cancer cells were indicated by methylthiazolyl tetrazolium (MTT) assay. In vivo experiments also revealed its superior anticancer effect compared with free GEM treatment. Therefore, the combination of polymer coated NDs with high surface capability and enzyme-responsive intracellular GEM release make it possible to realize higher treatment efficiency on pancreatic tumor therapy.
- Published
- 2020
245. 3-Bromopyruvate-Conjugated Nanoplatform-Induced Pro-Death Autophagy for Enhanced Photodynamic Therapy against Hypoxic Tumor
- Author
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Xiaohui Chen, Yongyan Deng, Pengyu Song, Jian Ji, Qiao Jin, Liangjie Hong, and Yue Huang
- Subjects
Porphyrins ,medicine.medical_treatment ,General Physics and Astronomy ,Photodynamic therapy ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Metastasis ,Cell Line, Tumor ,Neoplasms ,Autophagy ,medicine ,Humans ,General Materials Science ,Photosensitizer ,Hypoxia ,Pyruvates ,chemistry.chemical_classification ,Reactive oxygen species ,Photosensitizing Agents ,Tumor hypoxia ,Cell growth ,Chemistry ,General Engineering ,021001 nanoscience & nanotechnology ,medicine.disease ,0104 chemical sciences ,Photochemotherapy ,Apoptosis ,Cancer research ,0210 nano-technology - Abstract
Autophagy triggered by reactive oxygen species (ROS) in photodynamic therapy (PDT) generally exhibits an anti-apoptotic effect to promote cell survival. Herein, an innovative supramolecular nanoplatform was fabricated for enhanced PDT by converting the role of autophagy from pro-survival to pro-death. The respiration inhibitor 3-bromopyruvate (3BP), which can act as an autophagy promoter and hypoxia ameliorator, was integrated into photosensitizer chlorin e6 (Ce6)-encapsulated nanoparticles to combat hypoxic tumor. 3BP could inhibit respiration by down-regulating HK-II and GAPDH expression to significantly reduce intracellular oxygen consumption rate, which could relieve tumor hypoxia for enhanced photodynamic cancer therapy. More importantly, the autophagy level was significantly elevated by the combination of 3BP and PDT determined by Western blot, immunofluorescent imaging, and transmission electron microscopy. It was very surprising that excessively activated autophagy promoted cell apoptosis, leading to the changeover of autophagy from pro-survival to pro-death. Therefore, PDT combined with 3BP could achieve efficient cell proliferation inhibition and tumor regression. Furthermore, hypoxia-inducible factor-1α (HIF-1α) could be down-regulated after tumor hypoxia was relieved by 3BP. Tumor metastasis could then be effectively inhibited by eliminating primary tumors and down-regulating HIF-1α expression. These results provide an inspiration for future innovative approaches of cancer therapy by triggering pro-death autophagy.
- Published
- 2020
246. Nitrogen and phosphorus co-starvation inhibits anthocyanin synthesis in the callus of grape berry skin
- Author
-
Jiangfei Meng, Hui-Zhen Zheng, Xu-Qiao Jin, Huan Wei, Yulin Fang, Ming-Xin Feng, Teng-Fei Xu, Zhenwen Zhang, Xin Yang, and Shui-Huan Guo
- Subjects
0106 biological sciences ,Starvation ,Phosphorus ,fungi ,food and beverages ,chemistry.chemical_element ,Horticulture ,Biology ,01 natural sciences ,Nitrogen ,carbohydrates (lipids) ,chemistry.chemical_compound ,Pigment ,chemistry ,Biosynthesis ,Anthocyanin ,visual_art ,Callus ,visual_art.visual_art_medium ,medicine ,medicine.symptom ,Abscisic acid ,010606 plant biology & botany - Abstract
Anthocyanins are a type of natural pigment that have high potential for development and utilization in regions like food, pharmaceutical, and cosmetic industries, with nitrogen and phosphorus starvation possibly promoting their accumulation in grapes. However, it remains unclear whether such starvation impacts the grape callus, or how the co-starvation of nitrogen and phosphorus affects the biosynthesis of anthocyanins. Here, we investigated how nitrogen starvation, phosphorus starvation, and the co-starvation of these two elements affects the synthesis of anthocyanins in the callus of grape skin. Results showed that separate starvation of nitrogen and phosphorus, as well as nitrogen and phosphorus co-starvation, inhibited callus growth, while significantly promoting the accumulation of anthocyanins. However, co-starvation did not facilitate anthocyanin biosynthesis during the later stages of callus growth. qRT-PCR analysis showed that the expression of VvUFGT and VvMYBA1 was closely related to anthocyanin accumulation in the callus under nitrogen and phosphorus starvation. Besides, we also confirmed that the abscisic acid signaling pathway was involved in anthocyanin accumulation as well as callus resistance under adverse conditions. This study provides a basis for investigating the regulatory mechanisms of anthocyanin synthesis in grapes, as well as theoretical support for the production of anthocyanins by callus culture. Even though nitrogen and phosphorus deficiencies have been reported to promote anthocyanin biosynthesis in grapevine berries, whether the same deficiencies also induce anthocyanin accumulation in grape callus and whether nitrogen and phosphorus co-deficiency enhances this induction have yet to be reported. Therefore, the present study to investigate the effects of nitrogen starvation, phosphorus starvation, and nitrogen and phosphorus co-starvation on anthocyanin accumulation and ABA signaling in grape callus.
- Published
- 2020
247. Wire Desorption Combined with Electrospray Ionization Mass Spectrometry: Direct Analysis of Small Organic and Large Biological Compounds
- Author
-
Wenjian Sun, Yuanlong Wang, Jentaie Shiea, and Qiao Jin
- Subjects
Electrospray ,Atmospheric pressure ,Structural Biology ,Chemistry ,Desorption ,Electrospray ionization ,Ionization ,Thermal desorption ,Analytical chemistry ,Mass spectrometry ,Spectroscopy ,Ion source - Abstract
A novel atmospheric pressure ionization mass spectrometry based on wire desorption and electrospray ionization (WD-ESI) for direct analysis was developed to characterize chemical compounds with different polarities and thermal stabilities at atmospheric pressure. This technique is a variant of the thermal desorption electrospray ion source developed by Shiea et al. One large improvement is that the heating speed (>500 °C/s) of the thermal desorption in this work is extremely fast, using a self-heating metal wire, with which sample solution can splash from the surface to form small droplets and thus the analytes can be protected from thermal decomposition. With this feature, we have successfully achieved soft ionization of highly polar organic and biological compounds such as aflatoxin, small peptides, and even large proteins from complex matrices. The simple structure and self-cleaning capability of the WD-ESI source make it ideal for on-site screening in various applications such as food safety and biodrug testing, especially when coupled with a transportable mass spectrometer.
- Published
- 2020
248. Ofloxacin loaded MoS2 nanoflakes for synergistic mild-temperature photothermal/antibiotic therapy with reduced drug resistance of bacteria
- Author
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Jian Ji, Qiang Gao, Yifan Gao, Qiao Jin, Haijie Han, Ke Yao, Xu Li, and Yue Huang
- Subjects
Combination therapy ,Chemistry ,medicine.drug_class ,Antibiotics ,02 engineering and technology ,Drug resistance ,Photothermal therapy ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,medicine.disease_cause ,01 natural sciences ,Combinatorial chemistry ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,Antibiotic resistance ,Staphylococcus aureus ,Dispersion stability ,medicine ,General Materials Science ,Ofloxacin ,Electrical and Electronic Engineering ,0210 nano-technology ,medicine.drug - Abstract
Antibiotic resistance is an increasingly serious threat to global public health, which can lead to the decrease of the effectiveness of antibiotics. The combination therapy of antibiotic and mild temperature photothermal therapy (PTT) is adopted to address this issue in this work. An antibiotic-loaded nanoplatform is fabricated based on two-dimensional (2D) molybdenum disulfide (MoS2) nanoflakes as effective near-infrared (NIR) photothermal agent. The MoS2 nanoflakes is modified with positively charged quaternized chitosan (QCS) to improve the dispersion stability and enhance the interaction between MoS2 nanoflakes and bacterial membrane. The QCS modified MoS2 nanoflakes (QCS-MoS2) is expected to adhere onto the membrane of methicillin-resistant Staphylococcus aureus (MRSA) and depolarize the bacterial membrane by local hyperthermia under NIR irradiation. A first-line antibiotic, ofloxacin (OFLX), can be loaded onto QCS-MoS2 by π-π stacking and hydrophobic interaction. Due to the combined antibiotic-photothermal therapy, superior bactericidal ability was achieved at mild temperature (45 °C) and low antibiotic concentration. Such synergistic mild-temperature photothermal/antibiotic therapy can not only avoid the damage to neighboring tissue by PTT, but also reduce the development of drug resistance, providing an innovative way for the treatment of bacterial infections.
- Published
- 2020
249. Structure-Switchable DNA Programmed Disassembly of Nanoparticles for Smart Size Tunability and Cancer-Specific Drug Release
- Author
-
Xiaohui Chen, Fan Jia, Qiao Jin, Jian Ji, Wanying Ye, Yueming Liu, and Xu Li
- Subjects
Materials science ,Metal Nanoparticles ,Nanoparticle ,Antineoplastic Agents ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,chemistry.chemical_compound ,In vivo ,Cell Line, Tumor ,Neoplasms ,medicine ,Animals ,Humans ,General Materials Science ,Doxorubicin ,Telomerase ,Drug Carriers ,Mice, Inbred BALB C ,Tumor microenvironment ,Inverted Repeat Sequences ,DNA ,Penetration (firestop) ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,0104 chemical sciences ,Drug Liberation ,chemistry ,Colloidal gold ,Biophysics ,Nucleic Acid Conformation ,Female ,Gold ,Drug Screening Assays, Antitumor ,Nanocarriers ,0210 nano-technology ,medicine.drug - Abstract
The size of the nanocarrier is considered one of the most important issues for its therapeutic effect. Thus, an intelligent nanocarrier with dynamic size has been explored as a promising approach to fulfill the requirements for both efficient accumulation according to the enhanced penetration and retention (EPR) effect and deep penetration into tumor tissue. Herein, structure-switchable triplex DNA was modified on gold nanoparticles (AuNPs) to investigate its potential to modulate the nanoparticle dynamic disassembly process among the tumor microenvironment. We report that the pH-sensitive triplex DNA exhibited outstanding sensitivity and size tunability in triggering the disassembly of AuNP clusters into smaller sizes among the tumor acidic environment, leading to better permeability both in vitro and in vivo. By further combination of the telomerase-sensitive hairpin DNA loaded with chemotherapy drug doxorubicin (DOX), a cancer-specific intracellular drug-release function was also realized, resulting in a precise treatment effect and lower toxicity on normal cells. Through comodification of these two structure-switchable DNA chains on AuNPs and construction of nanoparticle assemblies with proper size, programmed disassembly and drug-release function in tissue and cell level, respectively, were successfully combined and eventually facilitated a highly efficient nanodrug transportation process, from tumor accumulation to deep penetration and precise cancer chemotherapy. The study provided the prospect of utilizing functionalized DNA in optimization of nanocarrier delivery efficiency.
- Published
- 2020
250. Supramolecular Aggregation-Induced Emission Nanodots with Programmed Tumor Microenvironment Responsiveness for Image-Guided Orthotopic Pancreatic Cancer Therapy
- Author
-
Jian Ji, Heqi Gao, Yongyan Deng, Qiao Jin, Dan Ding, and Xiaohui Chen
- Subjects
Supramolecular chemistry ,General Physics and Astronomy ,02 engineering and technology ,Enhanced permeability and retention effect ,Conjugated system ,010402 general chemistry ,01 natural sciences ,Drug Delivery Systems ,Pancreatic cancer ,Tumor Microenvironment ,medicine ,Humans ,General Materials Science ,Drug Carriers ,Tumor microenvironment ,Chemistry ,General Engineering ,021001 nanoscience & nanotechnology ,medicine.disease ,0104 chemical sciences ,Pancreatic Neoplasms ,Drug delivery ,Cancer cell ,Biophysics ,Matrix Metalloproteinase 2 ,0210 nano-technology ,Drug carrier - Abstract
Supramolecular nanomaterials as drug carriers have recently received increasing attention due to their intrinsic merits such as high stability, strong inclusion capability, and facile modification of the parental structure; however, intelligent ones with combined capacities of long blood circulation, highly efficient tumor cell uptake, and site-oriented drug release inside tumor cells are still rather limited. Herein, we report a strategy using supramolecular aggregation-induced emission (AIE) nanodots for image-guided drug delivery, which integrate both the advantages of AIE and supramolecular nanomaterials. The supramolecular AIE dots are prepared by the host-guest coassembly of the matrix metalloproteinase-2 (MMP-2) sensitive PEG-peptide (PEG2000-RRRRRRRR (R8)-PLGLAG-EKEKEKEKEKEK (EK6)) and functional α-cyclodextrins (α-CD) derivatives that are conjugated with the anticancer drug gemcitabine (GEM) and a far-red/near-infrared fluorescent rhodanine-3-acetic acid-based AIE luminogen, respectively. The supramolecular AIE dots realize long blood circulation time by virtue of the zwitterionic stealth peptide EK6. After largely accumulating in tumor tissues by the enhanced permeability and retention effect, the supramolecular AIE dots can successively respond to the tumor-overexpressed MMP-2 and intracellular reductive microenvironment, achieving both enhanced cancer cellular uptake and selective GEM release within cancer cells, which thus exhibit excellent tumor inhibition ability in both subcutaneous and orthotopic pancreatic tumor models.
- Published
- 2020
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