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Circ_ROBO2/miR-149 Axis Promotes the Proliferation and Migration of Human Aortic Smooth Muscle Cells by Activating NF-κB Signaling
- Source :
- Cytogenetic and Genome Research. 161:414-424
- Publication Year :
- 2021
- Publisher :
- S. Karger AG, 2021.
-
Abstract
- Atherosclerosis is the leading global cause of mortality. The occurrence of coronary artery disease (CAD) is regulated by a diversity of pathways, including circRNAs. However, the potential mechanisms of circRNAs in CAD remain unclear. Here, qRT-PCR was used to examine the expressions of miR-149 and circ_ROBO2. Their influences on cell proliferation, migration, and apoptosis were measured by CCK-8, trans­well, and flow cytometry assays, respectively. The protein levels of p-IκBα and NF-κB p65 were examined using western blot. The molecular interactions were validated using dual luciferase reporter and RNA pull-down assays. The expression patterns of circ_ROBO2 and miR-149 in CAD patients and PDGF-BB-treated human aortic smooth muscle cells (HASMCs) were upregulated and downregulated, respectively. Knockdown of circ_ROBO2 could markedly inhibit the capabilities of proliferation and migration, enhance the apoptotic rate, and suppress NF-κB signaling in PDGF-BB-treated HASMCs. Mechanistically, circ_ROBO2 acted as a sponge of miR-149 to activate TRAF6/NF-κB signaling. Rescue studies demonstrated that neither silencing miR-149 nor activation of NF-κB signaling obviously abolished the biological roles of circ_ROBO2 knockdown in PDGF-BB treated-HASMCs. This discovery elucidated a functional mechanism of circ_ROBO2 in CAD, suggesting that circRNAs serve a vital role in the progression of CAD.
- Subjects :
- Gene knockdown
medicine.diagnostic_test
Cell growth
Myocytes, Smooth Muscle
Becaplermin
NF-kappa B
RNA
RNA, Circular
Biology
Flow cytometry
Cell biology
MicroRNAs
Downregulation and upregulation
Western blot
Cell Movement
Apoptosis
Genetics
medicine
Humans
Gene silencing
Receptors, Immunologic
Molecular Biology
Genetics (clinical)
Cell Proliferation
Subjects
Details
- ISSN :
- 1424859X and 14248581
- Volume :
- 161
- Database :
- OpenAIRE
- Journal :
- Cytogenetic and Genome Research
- Accession number :
- edsair.doi.dedup.....7f7e21d17b83aa0d9b41f237def2fb93