46 results on '"Dongdong Su"'
Search Results
2. Spatial Magnetic-Field Description Method Aimed at 2 × 25 kV Auto-Transformer Power Supply System in High-Speed Railway
- Author
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Yunchuan Deng, Ke Huang, Dongdong Su, and Zhigang Liu
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high-speed railway (HSR) ,spatial magnetic-field description method ,auto-transformer (AT) power supply ,real loop circuits ,traction network integrated impedance ,current distribution ,Technology ,Engineering (General). Civil engineering (General) ,TA1-2040 ,Biology (General) ,QH301-705.5 ,Physics ,QC1-999 ,Chemistry ,QD1-999 - Abstract
Complete and accurate spatial magnetic field description is the premise of effectively assessing the power supply capability of a high-speed railway (HSR). Its evaluation indicators are the current distributions and the integrated impedance of traction network. This paper proposes a spatial magnetic-field description method for the auto-transformer (AT) power supply system. Due to the limitations of previous approaches, all the real loop circuits of the AT system are considered for structuring a loop circuit matrix. At first, different description processes are divided, respectively, into those for the right side and the left side of the load. Then, considering that two types of return conductors exist in an AT system, a certain current ratio deduced in existing studies is introduced. As the introduced current ratio of the left side of the load is approximate, an iterative scheme is adopted. By constantly adjusting current ratio to satisfy a constraint condition of circuit voltages, accurate traction network impedance is obtained. Last, in order to verify the effectiveness of the proposed description method, two real-life experiments in a Chinese HSR line are performed, which indicate that the proposed method can not only directly reflect complete and accurate current distribution, but also deduce the exact traction network integrated impedance.
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- 2018
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3. Enzyme-activated near-infrared fluorogenic probe with high-efficiency intrahepatic targeting ability for visualization of drug-induced liver injury†
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Yaling Wang, Mingrui Li, Yong Zhang, Xueqian Chen, Xueyun Gao, Dongdong Su, Qing Yuan, and Yongning Bian
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Liver injury ,chemistry.chemical_classification ,Drug ,Fluorophore ,media_common.quotation_subject ,digestive, oral, and skin physiology ,Cholic acid ,General Chemistry ,medicine.disease ,chemistry.chemical_compound ,Chemistry ,Liver targeting ,Enzyme ,chemistry ,In vivo ,medicine ,Biophysics ,Leucine ,human activities ,media_common - Abstract
Hepatotoxicity is a serious problem faced by thousands of clinical drugs, and drug-induced liver injury (DILI) caused by chronic administration or overdose has become a major biosafety issue. However, the near-infrared (NIR) fluorescent probes currently used for liver injury detection still suffer from poor liver targeting ability and low sensitivity. Enzyme-activated fluorogenic probes with powerful in situ targeting ability are the key to improving the imaging effect of liver injury. Herein, we rationally designed a leucine aminopeptidase (LAP) activated fluorogenic probe hCy-CA-LAP, which greatly improved the hepatocyte-targeting capability by introducing a cholic acid group. The probe hCy-CA-LAP is converted into a high-emission hCy-CA fluorophore in the presence of LAP, showing high selectivity, high sensitivity and low detection limit (0.0067 U mL−1) for LAP, and successfully realizes the sensitive detection of small fluctuations of LAP in living cells. Moreover, the probe can achieve effective in situ accumulation in the liver, thereby achieving precise imaging and evaluation of two different types of drug-induced hepatotoxicity in vivo. Therefore, the probe hCy-CA-LAP may be a potential tool for exploring the roles of LAP and evaluating the degree of DILI., We rationally designed a leucine aminopeptidase (LAP) activated fluorogenic probe hCy-CA-LAP with high hepatocyte-targeting ability for accurate and sensitive imaging of DILI.
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- 2021
4. Cell membranes targeted unimolecular prodrug for programmatic photodynamic-chemo therapy
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Dongdong Su, Jie Yuan, Xiao-Bing Zhang, Xiujuan Zhuang, Hepeng Zhao, Xing-Xing Zhang, Mei Chen, Lin Yuan, and Rong Peng
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Combination therapy ,medicine.medical_treatment ,Cell ,Medicine (miscellaneous) ,Photodynamic therapy ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,two-photon photodynamic therapy ,combination therapy ,In vivo ,medicine ,Animals ,Humans ,Prodrugs ,Photosensitizer ,glutathione ,Hypoxia ,Pharmacology, Toxicology and Pharmaceutics (miscellaneous) ,Fluorescent Dyes ,Photons ,Chemotherapy ,Photosensitizing Agents ,Singlet Oxygen ,Staining and Labeling ,Tumor hypoxia ,Rhodamines ,Chemistry ,Cell Membrane ,Mammary Neoplasms, Experimental ,Prodrug ,021001 nanoscience & nanotechnology ,humanities ,0104 chemical sciences ,medicine.anatomical_structure ,Photochemotherapy ,Cancer research ,Female ,0210 nano-technology ,Azo Compounds ,Research Paper ,HeLa Cells - Abstract
Photodynamic therapy (PDT) has emerged as one of the most up-and-coming non-invasive therapeutic modalities for cancer therapy in rencent years. However, its therapeutic effect was still hampered by the short life span, limited diffusion distance and ineluctable depletion of singlet oxygen (1O2), as well as the hypoxic microenvironment in the tumor tissue. Such problems have limited the application of PDT and appropriate solutions are highly demand. Methods: Herein, a programmatic treatment strategy is proposed for the development of a smart molecular prodrug (D-bpy), which comprise a two-photon photosensitizer and a hypoxia-activated chemotherapeutic prodrug. A rhodamine dye was designed to connect them and track the drug release by the fluorescent signal generated through azo bond cleavage. Results: The prodrug (D-bpy) can stay on the cell membrane and enrich at the tumor site. Upon light irradiation, the therapeutic effect was enhanced by a stepwise treatment: (i) direct generation of 1O2 on the cell membrane induced membrane destruction and promoted the D-bpy uptake; (ii) deep tumor hypoxia caused by two-photon PDT process further triggered the activation of the chemotherapy prodrug. Both in vitro and in vivo experiments, D-bpy have exhabited excellent tumor treatment effect. Conclusion: The innovative programmatic treatment strategy provides new strategy for the design of follow-up anticancer drugs.
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- 2021
5. Peptide and protein modified metal clusters for cancer diagnostics
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Xueyun Gao, Dongdong Su, Xiangchun Zhang, Fuping Gao, and Liang Gao
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Chemistry ,Low toxicity ,Computer science ,Nanotechnology ,General Chemistry ,Materials design ,Metal clusters - Abstract
A perspective on the development of peptide and protein modified metal clusters in cancer diagnostic applications., The biomedical features of metal clusters have been explored in tumor diagnostic applications in recent years. Peptide or protein protected metal clusters with low toxicity, ultra-small size and good biocompatibility are ideal bioanalytical tools, and exhibit better cancer diagnostic properties that have been attractive to oncologists. This perspective provides a rigorous but succinct overview of cancer diagnosis as a working concept for metal clusters by reporting the latest significant advances in the applications of metal clusters in tumor-related bioanalysis and diagnosis. The materials design principles, bioanalytical mechanisms and biomedical applications of metal clusters are described, and then the potential challenges and prospects of metal clusters in cancer diagnosis are discussed. A perspective addressing the role of metal clusters in this field is required to understand their effects and functions, as well as for the scientific community to further advance the development of metal clusters for broader diagnostic applications.
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- 2020
6. Noble-metal nanocluster as enzyme-mimetic catalyst for diagnostic analysis
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Dongdong Su, Qing Yuan, ShiZheng Wang, Xueyun Gao, Liang Gao, and Xiaojun Ren
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chemistry.chemical_classification ,Enzyme ,chemistry ,Diagnostic analysis ,General Engineering ,engineering ,General Materials Science ,Noble metal ,engineering.material ,Combinatorial chemistry ,Catalysis - Published
- 2019
7. Recent advances in molecular fluorescent probes for organic phosphate biomolecules recognition
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Dongdong Su, Shulu Yin, Xiangyang Gong, Wei Li, Lin Yuan, Xiao-Bing Zhang, and Xiaopeng Fan
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chemistry.chemical_classification ,Biomolecule ,Signal modulation ,Nanotechnology ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,chemistry ,Nucleic acid ,0210 nano-technology - Abstract
Organic phosphate biomolecules (OPBs) are indispensable components of eukaryotes and prokaryotes, such as acting as the fundamental components of cell membranes and important substrates for nucleic acids. They play pivotal roles in various biological processes, such as energy conservation, metabolism, and signal modulation. Due to the difficulty of detection caused by variety OPBs, investigation of their respective physiological effects in organisms has been restrained by the lack of efficient tools. Many small fluorescent probes have been employed for selective detection and monitoring of OPBs in vitro or in vivo due to the advantages of tailored properties, biodegradability and in situ high temporal and spatial resolution imaging. In this review, we summarize the recent advances in fluorescent probes for OPBs, such as nucleotides, NAD(P)H, FAD/FMN and PS. Importantly, we describe their identification mechanisms in detail and discuss the general strategies for these OPBs probe designs, which provide new insights and ideas for the future probe designs.
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- 2019
8. A fast responsive chromogenic and near-infrared fluorescence lighting-up probe for visual detection of toxic thiophenol in environmental water and living cells
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Ruilong Sheng, Juanjuan Wu, Wei Li, Lintao Zeng, João Rodrigues, Caiqin Qin, and Dongdong Su
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Infrared Rays ,02 engineering and technology ,Wastewater ,Photochemistry ,01 natural sciences ,Chloride ,Fluorescence ,Analytical Chemistry ,chemistry.chemical_compound ,Phenols ,Environmental water ,Limit of Detection ,Oxazines ,medicine ,Humans ,Sulfhydryl Compounds ,Fluorescent Dyes ,Detection limit ,Sulfonamides ,Microscopy, Confocal ,Chromogenic ,Thiophenol ,010401 analytical chemistry ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,Nile blue ,0104 chemical sciences ,Spectrometry, Fluorescence ,Models, Chemical ,chemistry ,0210 nano-technology ,Selectivity ,Water Pollutants, Chemical ,HeLa Cells ,medicine.drug - Abstract
Thiophenols as high toxic environmental pollutants are poisonous for animals and aquatic organisms. Therefore, it is indispensable to monitor thiophenols in the environment. Herein, a novel near-infrared fluorescent probe was developed for the detection of thiophenols, which was easily prepared by one-step coupling of 2,4-dinitrobenzenesulfonyl chloride with Nile blue. The probe showed a significant near infrared (∼675 nm) fluorescence “turn-on” response to thiophenols with some good features including chromogenic reaction, high sensitivity and selectivity, fast response, near-infrared emission along with low detection limit (1.8 nM). The probe was employed to rapidly and visually determine thiophenols in several industrial wastewaters with good recoveries (90–110%). Moreover, this probe has been demonstrated good capability for imaging thiophenol in HeLa cells.
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- 2019
9. Visualizing Microglia with a Fluorescence Turn‐On Ugt1a7c Substrate
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Florent Ginhoux, Jin-Soo Kim, Young-Tae Chang, Aymeric Silvin, Sungjin Park, H. Shawn Je, Masahiro Fukuda, Audrey Tze Ting Khoo, Xiao Liu, Beomsue Kim, Sejong Choi, Taejoon Kwon, Jungyeol Lee, Xiaogang Liu, Dongdong Su, Weijie Chi, Diana S. Y. Wan, and Srikanta Sanu
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Genetically modified mouse ,Brain development ,Microglia ,010405 organic chemistry ,Chemistry ,Optical Imaging ,Chemical probe ,General Chemistry ,010402 general chemistry ,01 natural sciences ,Fluorescence ,Catalysis ,In vitro ,0104 chemical sciences ,Mice ,medicine.anatomical_structure ,nervous system ,In vivo ,medicine ,Animals ,Macrophage ,Glucuronosyltransferase ,Neuroscience ,Fluorescent Dyes - Abstract
Microglia, the brain-resident macrophage, are involved in brain development and contribute to the progression of neural disorders. Despite the importance of microglia, imaging of live microglia at a cellular resolution has been limited to transgenic mice. Efforts have therefore been dedicated to developing new methods for microglia detection and imaging. Using a thorough structure-activity relationships study, we developed CDr20, a high-performance fluorogenic chemical probe that enables the visualization of microglia both in vitro and in vivo. Using a genome-scale CRISPR-Cas9 knockout screen, the UDP-glucuronosyltransferase Ugt1a7c was identified as the target of CDr20. The glucuronidation of CDr20 by Ugt1a7c in microglia produces fluorescence.
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- 2019
10. A distinctive near-infrared fluorescence turn-on probe for rapid, sensitive and chromogenic detection of sulfite in food
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Chong Duan, Guang-Ming Bao, Dongdong Su, Lintao Zeng, Jun-Feng Zhang, and Yubo Hu
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Chromatography ,food.ingredient ,Chromogenic ,Process Chemistry and Technology ,General Chemical Engineering ,Food additive ,food and beverages ,02 engineering and technology ,Near infrared fluorescence ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,chemistry.chemical_compound ,Residue (chemistry) ,Visual detection ,food ,chemistry ,Sulfite ,0210 nano-technology ,Sodium sulfite - Abstract
Sodium sulfite (Na2SO3) is widely used as a food additive, but the excessive residue of SO32− in food can cause irritative effects and damages to the human body. In this study, we reported a novel near-infrared (NIR) fluorescent probe (DCMQ) for visual detection of SO32− based on a chromogenic reaction. The probe can quantitatively determine SO32− with high specificity and sensitivity, fast response (
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- 2019
11. A smart fluorescent probe for discriminative detection of hydrazine and bisulfite from different emission channels
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Juanjuan Wu, Dongdong Su, Zhuo Ye, Jian Pan, and Lintao Zeng
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Detection limit ,High selectivity ,Hydrazine ,Metals and Alloys ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,01 natural sciences ,Combinatorial chemistry ,Fluorescence ,Ratiometric fluorescence ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Bisulfite ,chemistry.chemical_compound ,Human health ,chemistry ,Materials Chemistry ,Electrical and Electronic Engineering ,0210 nano-technology ,Instrumentation - Abstract
Hydrazine and bisulfite are poisonous to the environment and human health, hence it is necessary to monitor hydrazine and bisulfite in the environment and living cells. In this study, we reported a smart fluorescent probe (CMCA) for discriminative detection of hydrazine and bisulfite. The single fluorescent probe can respond to hydrazine and bisulfite with two different sets of colorimetric and ratiometric fluorescence signals together with high selectivity, fast response and low detection limit. The discriminative sensing behaviors of the probe for hydrazine and bisulfite were ascribed to different reaction mechanisms, which were confirmed by 1H NMR and HR-MS spectra. The probe has been successfully used to discriminatively determine hydrazine and bisulfite in industrial wastewater with good recoveries. Furthermore, the probe was employed to visualize hydrazine and bisulfite in live cells from different fluorescence channels with good performance. Therefore, this probe has great potential applications for discriminative detection of hydrazine and bisulfite in the environment and living cells.
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- 2018
12. Diversity-Oriented Fluorescence Library Approach (DOFLA) for Discovery of Cell-Permeable Probes for Applications in Live Cell Imaging
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Dongdong Su and Young-Tae Chang
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medicine.anatomical_structure ,Chemistry ,Live cell imaging ,Cell ,medicine ,Fluorescence ,Cell biology - Published
- 2021
13. Target identification of a macrocyclic hexaoxazole G-quadruplex ligand using post-target-binding visualization
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Young-Tae Chang, Yuki Wakabayashi, Kazuo Nagasawa, Yue Ma, Hiroyuki Seimiya, Sachiko Okabe, Dongdong Su, Mizuho Yasuda, and Masayuki Tera
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Fluorophore ,Macrocyclic Compounds ,Stereochemistry ,Alkyne ,010402 general chemistry ,G-quadruplex ,Ligands ,01 natural sciences ,Catalysis ,03 medical and health sciences ,chemistry.chemical_compound ,Cell Line, Tumor ,Materials Chemistry ,Moiety ,Humans ,Oxazoles ,030304 developmental biology ,chemistry.chemical_classification ,0303 health sciences ,Binding Sites ,Molecular Structure ,Chemistry ,Ligand ,Metals and Alloys ,General Chemistry ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,G-Quadruplexes ,Ceramics and Composites ,Click chemistry ,Azide ,BODIPY - Abstract
Macrocyclic hexaoxazoles (6OTDs) are G-quadruplex (G4) ligands, and some derivatives, such as L2H2-6OTD (1a) bearing two aminobutyl side chains, show cytotoxicity towards cancer cells. To identify the cellular target of 1a, we employed a post-target-binding strategy utilizing click reaction (Huisgen cyclization) between the azide-conjugated ligand L2H2-6OTD-Az (1b) and the cell-permeable dye CO-1 bearing a strained alkyne moiety and the BODIPY fluorophore under Cu-free conditions. We confirmed that introduction of the small azide group did not alter the physical or biological properties, including anti-cancer activity, of 1a, and we also demonstrated bias-free localization of CO-1. The post-binding visualization strategy suggested that L2H2-6OTD (1a) colocalized with RNA G4 in living cells.
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- 2020
14. Visualization of oxidative injury in the mouse kidney using selective superoxide anion fluorescent probes† †Electronic supplementary information (ESI) available: Experimental procedures, characterization data, and additional spectra. See DOI: 10.1039/c8sc03308k
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Lv, Yun, Dan Cheng, Dongdong Su, Chen, Mei, Yin, Bin-Cheng, Yuan, Lin, and Zhang, Xiao-Bing
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Chemistry ,urogenital system ,urologic and male genital diseases ,female genital diseases and pregnancy complications - Abstract
Drug-induced acute kidney injury (AKI), caused by renal drug metabolism, has been regarded as a main problem in clinical pharmacology and practice., Drug-induced acute kidney injury (AKI), caused by renal drug metabolism, has been regarded as a main problem in clinical pharmacology and practice. However, due to the lack of effective biomarkers and noninvasive real-time tools, the early diagnosis of drug-induced AKI is still a crucial challenge. The superoxide anion (O2˙–), the preliminary reactive oxidative species, is closely related to drug-induced AKI. In this paper, we reported two new mitochondria-targeted fluorescent probes for investigating AKI via mapping the fluctuation of O2˙– with high sensitivity and selectivity by the combination of rational design and a probe-screening approach. Small-molecule fluorescent probes (Naph-O2˙– and NIR-O2˙–) with high accuracy and excellent selectivity were successfully applied to detect endogenously produced O2˙– in living cells and tissues by dual-model confocal imaging, and to trap the fluctuation of the O2˙– level during the drug-induced nephrotoxicity. Moreover, probe NIR-O2˙– was also used to elucidate the protective effects of l-carnitine (LC) against drug-induced nephrotoxicity for the first time. Therefore, these probes may be potential chemical tools for exploring the roles of O2˙– in complex nephrotoxicity disease systems.
- Published
- 2018
15. Highly photostable, lysosome-targeted BODIPYs with green to near-infrared emission for lysosome imaging in living cells
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Jian Pan, Dongdong Su, Chao Xiong, Lintao Zeng, and Zhuo Ye
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Fluorescence-lifetime imaging microscopy ,010405 organic chemistry ,Chemistry ,Process Chemistry and Technology ,General Chemical Engineering ,Near-infrared spectroscopy ,High selectivity ,010402 general chemistry ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,medicine.anatomical_structure ,Lysosome ,Biophysics ,medicine - Abstract
A series of highly photostable, lysosome-targeted BODIPYs with tunable fluorescence from green to the near-infrared region was developed. The probes were found to show an accurate lysosome-targeting ability with high selectivity and high photostability under continuous excitation irradiation. The results show that these lysosome-targeted BODIPYs offer a simple and convenient tool for fluorescence imaging of lysosomes, which will be valuable for understanding the activity and function of lysosomes in related cells.
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- 2018
16. Seeing Elastin: A Near-Infrared Zwitterionic Fluorescent Probe for In Vivo Elastin Imaging
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Jong-Jin Kim, Sungjin Park, Chai Lean Teoh, Myung Ae Bae, Renzhe Bi, U. S. Dinish, Michiya Matsusaki, Seong Soon Kim, Young-Tae Chang, Animesh Samanta, Malini Olivo, Marie Piantino, Dongdong Su, and Fiona Louis
- Subjects
0301 basic medicine ,Optical contrast ,biology ,Wavelength range ,Chemistry ,General Chemical Engineering ,Biochemistry (medical) ,Near-infrared spectroscopy ,Photoacoustic imaging in biomedicine ,General Chemistry ,Biochemistry ,Fluorescence ,Imaging modalities ,03 medical and health sciences ,030104 developmental biology ,In vivo ,Materials Chemistry ,biology.protein ,Biophysics ,Environmental Chemistry ,Elastin - Abstract
Summary Elastic fibers are present in a variety of tissues and are responsible for their resilience. Until now, no optical contrast agent in the near-infrared (NIR) wavelength range of 700–900 nm has been reported for the imaging of elastic fibers. Here, we report the discovery of a NIR zwitterionic elastin probe ElaNIR (elastin NIR) through fluorescent-image-based screening. The probe was successfully applied for in vitro , ex vivo , and in vivo imaging by various imaging modalities. Age-related elastin differences shown by in vivo fluorescent and photoacoustic imaging indicated that ElaNIR can be a potentially convenient tool for uncovering changes of elastin in live models.
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- 2018
17. Visualization of oxidative injury in the mouse kidney using selective superoxide anion fluorescent probes
- Author
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Bin-Cheng Yin, Yun Lv, Xiao-Bing Zhang, Dongdong Su, Mei Chen, Lin Yuan, and Dan Cheng
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chemistry.chemical_classification ,Reactive oxygen species ,010405 organic chemistry ,Chemistry ,Superoxide ,Rational design ,Acute kidney injury ,General Chemistry ,010402 general chemistry ,medicine.disease ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,Nephrotoxicity ,chemistry.chemical_compound ,Biochemistry ,medicine ,Mouse Kidney ,Oxidative injury - Abstract
Drug-induced acute kidney injury (AKI), caused by renal drug metabolism, has been regarded as a main problem in clinical pharmacology and practice. However, due to the lack of effective biomarkers and noninvasive real-time tools, the early diagnosis of drug-induced AKI is still a crucial challenge. The superoxide anion (O2˙-), the preliminary reactive oxidative species, is closely related to drug-induced AKI. In this paper, we reported two new mitochondria-targeted fluorescent probes for investigating AKI via mapping the fluctuation of O2˙- with high sensitivity and selectivity by the combination of rational design and a probe-screening approach. Small-molecule fluorescent probes (Naph-O2˙- and NIR-O2˙- ) with high accuracy and excellent selectivity were successfully applied to detect endogenously produced O2˙- in living cells and tissues by dual-model confocal imaging, and to trap the fluctuation of the O2˙- level during the drug-induced nephrotoxicity. Moreover, probe NIR-O2˙- was also used to elucidate the protective effects of l-carnitine (LC) against drug-induced nephrotoxicity for the first time. Therefore, these probes may be potential chemical tools for exploring the roles of O2˙- in complex nephrotoxicity disease systems.
- Published
- 2018
18. Detection of analytes in mitochondria without interference from other sites based on an innovative ratiometric fluorophore
- Author
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Tian-Bing Ren, Dongdong Su, Xiao-Bing Zhang, Xing-Xing Zhang, Qian-Ling Zhang, and Lin Yuan
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Analyte ,Fluorophore ,010405 organic chemistry ,General Chemistry ,Mitochondrion ,010402 general chemistry ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,Chemistry ,chemistry.chemical_compound ,Target site ,chemistry ,Interference (communication) ,Mitochondrial targeting ,Biophysics ,Reaction site - Abstract
A new strategy that integrates the targeting group and response moiety together for the preparation of mitochondrial probe was developed. Bioimaging studies have shown that for the first time, the newly designed probe HDFL-Cys can first accumulate in mitochondria and then react with the analyte., Mitochondria are vital organelles that not only produce cellular energy but also participate in many biological processes. Recently, various fluorescent probes have been developed for mitochondrial imaging. However, due to the lack of suitable dyes or strategies, it is difficult for most reported mitochondrial targeting probes to prove whether the analytes they detected are from mitochondria. In addition, positive charge on mitochondrial probes can seriously affect the mitochondrial environment. To address these issues, we herein put forward a novel strategy for probe design based on a smart NIR dye (HDFL) for mitochondrial targeting detection. Compared to general mitochondrial targeting probes that are modified with a target site and a reaction site, the new strategy is to combine the two sites together for a mitochondrial probe that would provide accurate detection of analytes in mitochondria without interference. As a proof of concept, we synthesized a mitochondrial-targetable probe HDFL-Cys for cysteine. Bioimaging studies have shown that the new type of probe HDFL-Cys can first accumulate in mitochondria and then react with the analyte (cysteine) accompanied by the departure of the targeting group (lipophilic cation moieties). Thus, it can specifically detect the analyte in mitochondria without interference from extra-mitochondrial analytes. We anticipate that the new strategy based on the novel NIR dye HDFL may be a potential platform for developing desirable ratiometric fluorescent probes for mitochondrial imaging.
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- 2018
19. A palette of background-free tame fluorescent probes for intracellular multi-color labelling in live cells
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Hee-Sung Park, Kyung Jin Lee, José L. Belmonte-Vázquez, Samira Husen Alamudi, Young-Tae Chang, Jung Yeol Lee, Eduardo Peña-Cabrera, and Dongdong Su
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chemistry.chemical_classification ,Bioconjugation ,010405 organic chemistry ,Chemistry ,Biomolecule ,General Chemistry ,010402 general chemistry ,01 natural sciences ,Fluorescence ,0104 chemical sciences ,chemistry.chemical_compound ,Labelling ,Biophysics ,Click chemistry ,Azide ,BODIPY ,Intracellular - Abstract
A multi-color labelling technique for visualizing multiple intracellular apparatuses in their native environment using small fluorescent probes remains challenging. This approach requires both orthogonal and biocompatible coupling reactions in heterogeneous biological systems with minimum fluorescence background noise. Here, we present a palette of BODIPY probes containing azide and cyclooctyne moieties for copper-free click chemistry in living cells. The probes, referred to as ‘tame probes’, are highly permeable and specific in nature, leaving no background noise in cells. Such probes, which are rationally designed through optimized lipophilicity, water solubility and charged van der Waals surface area, allow us to demonstrate rapid and efficient concurrent multi-labelling of intracellular target components. We show that these probes are capable of not only labelling organelles and engineered proteins, but also showing the intracellular glycoconjugates’ dynamics, through the use of metabolic oligosaccharide engineering technology in various cell types. The results demonstrated in this study thus provide flexibility for multi-spectral labelling strategies in native systems in a high spatiotemporal manner.
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- 2018
20. Real-Time In Vivo Hepatotoxicity Monitoring through Chromophore-Conjugated Photon-Upconverting Nanoprobes
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Xiaogang Liu, Animesh Samanta, Sungjin Park, Wenxuan Jiang, Juanjuan Peng, Angelo H. All, Xiao Zeng, Daniel Teh Boon Loong, Lu Wang, Nam-Young Kang, Sanyang Han, Dongdong Su, Lin Yuan, and Young-Tae Chang
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Modern medicine ,Nanotechnology ,General Medicine ,02 engineering and technology ,General Chemistry ,Conjugated system ,Chromophore ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Catalysis ,0104 chemical sciences ,Fever drug ,chemistry.chemical_compound ,chemistry ,In vivo ,Biophysics ,0210 nano-technology ,Luminescence ,Drug toxicity ,Peroxynitrite - Abstract
Drug toxicity is a long-standing concern of modern medicine. A typical anti-pain/fever drug paracetamol often causes hepatotoxicity due to peroxynitrite ONOO- . Conventional blood tests fail to offer real-time unambiguous visualization of such hepatotoxicity in vivo. Here we report a luminescent approach to evaluate acute hepatotoxicity in vivo by chromophore-conjugated upconversion nanoparticles. Upon injection, these nanoprobes mainly accumulate in the liver and the luminescence of nanoparticles remains suppressed owing to energy transfer to the chromophore. ONOO- can readily bleach the chromophore and thus recover the luminescence, the presence of ONOO- in the liver leads to fast restoring of the near-infrared emission. Taking advantages of the high tissue-penetration capability of near-infrared excitation/emission, these nanoprobes achieve real-time monitoring of hepatotoxicity in living animals, thereby providing a convenient screening strategy for assessing hepatotoxicity of synthetic drugs.
- Published
- 2017
21. A new approach for turn-on fluorescence sensing of l-DOPA
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Young-Tae Chang, Jungyeol Lee, Lu Wang, Dongdong Su, and Stuart N. Berry
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Chemistry ,Metals and Alloys ,Analytical chemistry ,Fluorescence sensing ,02 engineering and technology ,General Chemistry ,010402 general chemistry ,021001 nanoscience & nanotechnology ,01 natural sciences ,Catalysis ,nervous system diseases ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Turn (biochemistry) ,parasitic diseases ,Materials Chemistry ,Ceramics and Composites ,Biophysics ,0210 nano-technology ,Fluorescence response - Abstract
A novel design strategy for the fluorescence sensing of l-DOPA is reported. Resa-Sulf displays a significant turn-on fluorescence response to l-DOPA due to its reduction properties; this sensing mechanism was fully confirmed by mechanistic studies. Furthermore, Resa-Sulf was successfully utilized to quantitatively detect l-DOPA concentrations from a commercially available source.
- Published
- 2017
22. A unique off-on near-infrared QCy7-derived probe for selective detection and imaging of hydrogen sulfide in cells and in vivo
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Dongdong Su, Qian Wu, Dan Cheng, Yun Lv, Xiaojun Ren, and Lin Yuan
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Hydrogen sulfide ,Regulator ,Endogeny ,02 engineering and technology ,010402 general chemistry ,01 natural sciences ,Fluorescence spectroscopy ,Analytical Chemistry ,chemistry.chemical_compound ,Mice ,In vivo ,Materials Testing ,Animals ,Humans ,Hydrogen Sulfide ,Instrumentation ,Spectroscopy ,Fluorescent Dyes ,Mice, Inbred BALB C ,Spectroscopy, Near-Infrared ,Near-infrared spectroscopy ,Hep G2 Cells ,equipment and supplies ,021001 nanoscience & nanotechnology ,Fluorescence ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,Molecular Imaging ,chemistry ,Microscopy, Fluorescence ,Biophysics ,Female ,Single-Cell Analysis ,0210 nano-technology ,Selectivity ,HeLa Cells - Abstract
Hydrogen sulfide (H2S) has been found to be an important biological regulator that plays important roles in many physiological and pathological processes. Near-infrared (NIR) fluorescent probes capable of selectively detecting H2S in vivo will be useful tools to understand its mechanisms in biological processes. Herein, we reported an easily synthesized and stimuli-responsive NIR fluorescent probe (QCy7-HS) for selective evaluation of endogenous H2S in the living cells and mice. In response to cellular H2S stimulus, QCy7-HS is converted to QCy7 and shows a unique off-on near-infrared fluorescence signal change. The results of selectivity and kinetic studies indicated that our probe has high H2S binding capacity. Therefore, this probe was used for the fluorescence detection of H2S in cells. Moreover, the probe was applied for study exogenous and endogenous H2S in live mice, indicating that the new probe can be used as an efficient tool on H2S related events in living animals.
- Published
- 2019
23. A Near-Infrared Probe Tracks and Treats Lung Tumor Initiating Cells by Targeting HMOX2
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Hyung-Ho Ha, Sungjin Park, Jungyeol Lee, Xiao Liu, Nam-Young Kang, Jong-Jin Kim, Seong Soon Kim, Zhenxun Wang, Lu Wang, Haw-Young Kwon, Yong-An Lee, So Young Choi, Seong Cheol Hong, Animesh Samanta, Myung Ae Bae, Young Hyun Yu, Wai Leong Tam, Jun-Seok Lee, Young-Tae Chang, Junyoung Kim, Bing Lim, Jia Hui Jane Lee, Beomsue Kim, and Dongdong Su
- Subjects
congenital, hereditary, and neonatal diseases and abnormalities ,Lung Neoplasms ,HMOX2 ,010402 general chemistry ,01 natural sciences ,Biochemistry ,Catalysis ,Metastasis ,Mice ,Colloid and Surface Chemistry ,mental disorders ,medicine ,Animals ,Humans ,Fluorescent Dyes ,Spectroscopy, Near-Infrared ,biology ,Chemistry ,Cancer ,General Chemistry ,medicine.disease ,Ligand (biochemistry) ,Small molecule ,Xenograft Model Antitumor Assays ,nervous system diseases ,0104 chemical sciences ,body regions ,Heme oxygenase ,Survival Rate ,Heme Oxygenase (Decyclizing) ,Cancer research ,biology.protein ,Neoplastic Stem Cells ,Biomarker (medicine) ,human activities ,Preclinical imaging - Abstract
Tumor initiating cells (TIC) are resistant to conventional anticancer therapy and associated with metastasis and relapse in cancer. Although various TIC markers and their antibodies have been proposed, it is limited to the use of antibodies for in vivo imaging or treatment of TIC. In this study, we discovered heme oxygenase 2 (HMOX2) as a novel biomarker for TIC and developed a selective small molecule probe TiNIR (tumor initiating cell probe with near infrared). TiNIR detects and enriches the functionally active TIC in human lung tumors, and through the photoacoustic property, TiNIR also visualizes lung TIC in the patient-derived xenograft (PDX) model. Furthermore, we demonstrate that TiNIR inhibits tumor growth by blocking the function of HMOX2, resulting in significantly increased survival rates of the cancer model mice. The novel therapeutic target HMOX2 and its fluorescent ligand TiNIR will open a new path for the molecular level of lung TIC diagnosis and treatment.
- Published
- 2019
24. An ESIPT-Based Ratiometric Fluorescent Probe for Highly Sensitive and Rapid Detection of Sulfite in Living Cells
- Author
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Jianing Hou, Dan Cheng, Yu-Peng Liu, Tian-Bing Ren, Dongdong Su, and Lin Yuan
- Subjects
Detection limit ,Fluorescence-lifetime imaging microscopy ,Full Paper ,010405 organic chemistry ,General Chemistry ,Full Papers ,010402 general chemistry ,Photochemistry ,Condensation reaction ,01 natural sciences ,Fluorescence ,Rapid detection ,0104 chemical sciences ,Highly sensitive ,lcsh:Chemistry ,chemistry.chemical_compound ,sulfite ,Sulfite ,chemistry ,lcsh:QD1-999 ,imaging studies ,fluorescent probe ,Michael reaction ,fluorescence ,ratiometric probes - Abstract
The novel ratiometric fluorescent probe HPQRB with an ESIPT effect based on Michael addition for highly sensitive and fast detection of sulfite in living HepG2 cells is reported. HPQRB can be easily synthesized by a two‐step condensation reaction. HPQRB has a large emission shift (Δλ=116 nm), which is beneficial for fluorescence imaging research, and its sulfite‐responsive site is based on a rhodamine‐like structure with the emission peak at 566 nm, which decreases with increasing sulfite concentration. and its HPQ structure always has an ESIPT effect throughout the reaction process, keeping the emission peak at 450 nm as a self‐reference. In particular, HPQRB has high selectivity for sulfite and responds quickly (within 30 s) with a low detection limit (44 nM). Furthermore, HPQRB has been successfully used for fluorescence imaging of sulfite in HepG2 cells, demonstrating the superior ability to detect sulfite under physiological conditions.
- Published
- 2019
25. Frontispiece: Visualizing Microglia with a Fluorescence Turn‐On Ugt1a7c Substrate
- Author
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Audrey Tze Ting Khoo, Jungyeol Lee, Aymeric Silvin, H. Shawn Je, Beomsue Kim, Sungjin Park, Xiaogang Liu, Taejoon Kwon, Masahiro Fukuda, Xiao Liu, Weijie Chi, Dongdong Su, Sejong Choi, Diana S. Y. Wan, Florent Ginhoux, Srikanta Sanu, Young-Tae Chang, and Jin-Soo Kim
- Subjects
Turn (biochemistry) ,medicine.anatomical_structure ,Microglia ,Chemistry ,Biophysics ,medicine ,Substrate (chemistry) ,General Chemistry ,Fluorescence ,Catalysis - Published
- 2019
26. De Novo Design of Chemical Stability Near-Infrared Molecular Probes for High-Fidelity Hepatotoxicity Evaluation In Vivo
- Author
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Mei Chen, Juanjuan Peng, Lin Yuan, Dongdong Su, Dan Cheng, Xiao-Bing Zhang, Dongjie Liu, and Yun Lv
- Subjects
Fluorescence-lifetime imaging microscopy ,Cell Survival ,Infrared Rays ,010402 general chemistry ,Protective Agents ,01 natural sciences ,Biochemistry ,Catalysis ,Leucyl Aminopeptidase ,Colloid and Surface Chemistry ,In vivo ,Peroxynitrous Acid ,Humans ,Acetaminophen ,Fluorescent Dyes ,Detection limit ,Molecular Structure ,Chemistry ,Near-infrared spectroscopy ,Optical Imaging ,Rational design ,General Chemistry ,Hep G2 Cells ,Fluorescence ,0104 chemical sciences ,Biophysics ,Chemical stability ,Chemical and Drug Induced Liver Injury ,Molecular probe ,Biomarkers - Abstract
Near-infrared (NIR) fluorescence imaging technique is garnering increasing research attention due to various advantages. However, most NIR fluorescent probes still suffer from a false signals problem owing to their instability in real application. Especially in a pathological environment, many NIR probes can be easily destroyed due to the excessive generation of highly reactive species and causing a distorted false signal. Herein, we proposed an approach for developing a new stable NIR dye platform with an optically tunable group to eliminate false signals using the combination of dyes screening and rational design strategy. The conception is validated by the construction of two high-fidelity NIR fluorescent probes (NIR-LAP and NIR-ONOO–) sensing leucine aminopeptidase (LAP) and peroxynitrite (ONOO–), the markers of hepatotoxicity. These probes (NIR-LAP and NIR-ONOO–) were demonstrated to sensitively and accurately monitor LAP and ONOO– (detection limit: 80 mU/L for LAP and 90 nM for ONOO–), thereby allow...
- Published
- 2019
27. A Multisite-Binding Switchable Fluorescent Probe for Monitoring Mitochondrial ATP Level Fluctuation in Live Cells
- Author
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Beomsue Kim, Wang Xu, Yong Ni, Lu Wang, Jun Cheng Er, Xian Zeng, Bikram Keshari Agrawalla, Young-Tae Chang, Dongdong Su, Juanjuan Peng, and Lin Yuan
- Subjects
Nanotechnology ,Mitochondrion ,010402 general chemistry ,01 natural sciences ,Catalysis ,chemistry.chemical_compound ,Adenosine Triphosphate ,Cell Line, Tumor ,Humans ,Binding site ,Fluorescent Dyes ,Binding Sites ,010405 organic chemistry ,Chemistry ,Chemiosmosis ,General Chemistry ,General Medicine ,Fluorescence ,In vitro ,0104 chemical sciences ,Mitochondria ,Microscopy, Fluorescence ,Apoptosis ,Biophysics ,Adenosine triphosphate ,Intracellular - Abstract
Adenosine triphosphate (ATP), commonly produced in mitochondria, is required by almost all the living organisms; thus fluorescent probes for monitoring mitochondrial ATP levels fluctuation are essential and highly desired. Herein, we report a multisite-binding switchable fluorescent probe, ATP-Red 1, which selectively and rapidly responds to intracellular concentrations of ATP. Live-cell imaging indicated that ATP-Red 1 mainly localized to mitochondria with good biocompatibility and membrane penetration. In particular, with the help of ATP-Red 1, we successfully observed not only the decreased mitochondrial ATP levels in the presence of KCN and starvation state, but also the increased mitochondrial ATP levels in the early stage of cell apoptosis. These results indicate that ATP-Red 1 is a useful tool for investigating ATP-relevant biological processes.
- Published
- 2015
28. Development of a Highly Selective, Sensitive, and Fast Response Upconversion Luminescent Platform for Hydrogen Sulfide Detection
- Author
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Wang Xu, Chai Lean Teoh, Young-Tae Chang, Lu Wang, Juanjuan Peng, Xiaogang Liu, Dongdong Su, Animesh Samanta, Lin Yuan, and Xiao Zeng
- Subjects
Materials science ,Hydrogen sulfide ,Nanoparticle ,Nanotechnology ,02 engineering and technology ,equipment and supplies ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Highly selective ,01 natural sciences ,Photon upconversion ,0104 chemical sciences ,Electronic, Optical and Magnetic Materials ,Biomaterials ,Upconversion nanoparticles ,chemistry.chemical_compound ,chemistry ,Electrochemistry ,Monitoring methods ,0210 nano-technology ,Luminescence - Abstract
Hydrogen sulfide (H2S) has been recognized as one of most important gaseous signaling molecules mediated by a variety of physiological and pathological processes. Yet, its functions remain largely elusive due to the lack of potent monitoring methods. Hereby this issue is addressed with a powerful new platform—dye-assembled upconversion nanoparticles (UCNPs). A series of chromophores with different absorption bands and fast responses towards H2S is combined with UCNPs and results in a library of H2S sensors with responsive emission signals ranging from the visible to the near-infrared (NIR) region. These nanoprobes demonstrate highly selective and rapid responses to H2S in vitro and in cells. Furthermore, H2S levels in blood can be detected using the developed nanoprobes. Hence the reported H2S sensing platform can serve as a powerful diagnostic tool to research H2S functions and to investigate H2S-related diseases.
- Published
- 2015
29. A flexible paper-based chemosensor for colorimetric and ratiometric fluorescence detection of toxic oxalyl chloride
- Author
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Tianhong Chen, Yue Zhang, Hou-Qun Yuan, Lirong Jiang, Dongdong Su, and Guang-Ming Bao
- Subjects
Detection limit ,Anthracene ,Fluorophore ,Metals and Alloys ,02 engineering and technology ,Paper based ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Photochemistry ,01 natural sciences ,Fluorescence ,Ratiometric fluorescence ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,chemistry.chemical_compound ,Oxalyl chloride ,chemistry ,Intramolecular force ,Materials Chemistry ,Electrical and Electronic Engineering ,0210 nano-technology ,Instrumentation - Abstract
The screening of highly toxic chemical vapor is critical for environmental monitoring and human health maintenance. Herein, we reported a chemosensor (AC-HPD) for rapid and specific detection of highly toxic oxalyl chloride vapor, which adopted anthracene carboxyimide as a fluorophore and 3-aminopropanol as the reaction site. Upon coupling of oxalyl chloride with NH and OH groups, AC-HPD exhibited remarkable colorimetric and ratiometric fluorescence dual-mode responses towards oxalyl chloride via the intramolecular charge transfer effect. AC-HPD could determine oxalyl chloride with features of rapid response, excellent specificity, low detection limit (95 nM), as well as obvious color and fluorescence change. Furthermore, AC-HPD-embedded test strip was successfully fabricated and used as a flexible sensing device for rapid, sensitive and visible monitoring oxalyl chloride vapor.
- Published
- 2020
30. Colorimetric and Ratiometric Chemosensor for Visual Detection of Gaseous Phosgene Based on Anthracene Carboxyimide Membrane
- Author
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Qiao Hu, Chong Duan, Lintao Zeng, Ruilong Sheng, Juanjuan Wu, and Dongdong Su
- Subjects
Detection limit ,Anthracene ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Photochemistry ,01 natural sciences ,0104 chemical sciences ,Analytical Chemistry ,chemistry.chemical_compound ,Membrane ,Visual detection ,chemistry ,Polystyrene ,Phosgene ,0210 nano-technology ,Selectivity ,Rapid response - Abstract
In this work, we reported an anthracene carboxyimide-based chemosensor (AC-Phos) for colorimetric and ratiometric fluorescence detection of highly toxic phosgene, which displayed rapid response (
- Published
- 2018
31. Silica Nanoparticle-Enhanced Fluorescent Sensor Array for Heavy Metal Ions Detection in Colloid Solution
- Author
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Wang Xu, Junyao Li, Juanjuan Peng, Lu Wang, Dongdong Su, Chai Lean Teoh, and Young-Tae Chang
- Subjects
Metal ions in aqueous solution ,02 engineering and technology ,010402 general chemistry ,Mass spectrometry ,01 natural sciences ,Sensitivity and Specificity ,Analytical Chemistry ,Colloid ,Sensor array ,Limit of Detection ,Metals, Heavy ,Sensitivity (control systems) ,Irradiation ,Fluorescent Dyes ,Detection limit ,Chemistry ,business.industry ,021001 nanoscience & nanotechnology ,Silicon Dioxide ,Fluorescence ,0104 chemical sciences ,Spectrometry, Fluorescence ,Optoelectronics ,Nanoparticles ,0210 nano-technology ,business - Abstract
Sensitivity and detection limit are two vital factors that affect fluorophores-based sensing and imaging system. However, it remains a challenge to improve the sensitivity and detection limit of fluorophores, largely due to their limited response and photophysical properties. In this study, we report for the first time, a novel approach to enhance the sensitivity and detection limit of probes using silica nanoparticles, also known as silica nanoparticles-enhanced fluorescence (SiEF). SiEF can drastically improve the fluorescence intensities and detection limit of fluorophores. A SiEF-improved fluorescent sensor array for rapid and sensitive identification of different heavy metal ions is achieved, and a 3D spatial dispersion graph is obtained based on the SiEF-improved fluorescent sensor array, which provides a lower concentration dependent pattern than fluorophores alone, allowing qualitative, quantitative, and sensitive detection of heavy metal ions. Furthermore, with UV lamp irradiation of the sensor-metal ion mixtures, the output signals enable direct visual of heavy metal ions with low concentration. Thus, the SiEF approach provides a simple and practical strategy for fluorescent probes to improve their sensitivity and detection limit in analytes sensing.
- Published
- 2017
32. Chemical Fluorescent Probe for Detection of Aβ Oligomers
- Author
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Srikanta Sahu, Young-Tae Chang, Seong-Wook Yun, Sunhee Cho, Dongdong Su, Sihyun Ham, Frances Prelli, Chai Lean Teoh, Eleanor Drummond, Sulgi Lim, and Thomas Wisniewski
- Subjects
Models, Molecular ,Amyloid ,Fibril ,Biochemistry ,Oligomer ,Article ,Catalysis ,Mice ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,In vivo ,medicine ,Animals ,Fluorescent Dyes ,Amyloid beta-Peptides ,Chemistry ,Neurodegeneration ,Brain ,Fibrillogenesis ,General Chemistry ,medicine.disease ,Fluorescence ,In vitro ,Disease Models, Animal ,Thermodynamics - Abstract
Aggregation of amyloid β-peptide (Aβ) is implicated in the pathology of Alzheimer’s disease (AD), with the soluble, Aβ oligomeric species thought to be the critical pathological species. Identification and characterization of intermediate species formed during the aggregation process is crucial to the understanding of the mechanisms by which oligomeric species mediate neuronal toxicity and following disease progression. Probing these species proved to be extremely challenging, as evident by the lack of reliable sensors, due to their heterogeneous and transient nature. We describe here an oligomer-specific fluorescent chemical probe, BoDipy-Oligomer (BD-Oligo), developed through the use of the diversity-oriented fluorescent library approach (DOFLA) and high-content, imaging-based screening. This probe enables dynamic oligomer monitoring during fibrillogenesis in vitro and shows in vivo Aβ oligomers staining possibility in the AD mice model.
- Published
- 2015
33. A mitochondria-targeted ratiometric fluorescent probe to monitor endogenously generated sulfur dioxide derivatives in living cells
- Author
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Juanjuan Peng, Wang Xu, Dongdong Su, Lin Yuan, Chai Lean Teoh, and Young-Tae Chang
- Subjects
Indoles ,Biophysics ,Bioengineering ,Biosensing Techniques ,Mitochondrion ,complex mixtures ,Adduct ,Biomaterials ,HeLa ,chemistry.chemical_compound ,Sulfite ,Coumarins ,Cations ,Humans ,Sulfites ,Sulfur Dioxide ,Hydrogen Sulfide ,Fluorescent Dyes ,chemistry.chemical_classification ,biology ,biology.organism_classification ,Fluorescence ,Mitochondria ,respiratory tract diseases ,Amino acid ,Enzyme ,Microscopy, Fluorescence ,chemistry ,Biochemistry ,Mechanics of Materials ,Ceramics and Composites ,Intracellular ,HeLa Cells - Abstract
Sulfur dioxide (SO2) can be endogenously produced by enzymes in mitochondria during oxidation of H2S or sulphur-containing amino acids, and plays important roles in several physiological processes. However, the design and synthesis of fluorescent probes which can detect mitochondrial SO2 and its derivatives in living cells still remain unresolved. Herein, we report the preparation of a lipophilic cationic dye 1 (Mito-Ratio-SO2), which targets the mitochondria in living cells and is sensitive to the presence of SO2 derivatives. The ratiometric probe Mito-Ratio-SO2 displays a 170 nm blue-shift in emission with two well-resolved emission bands upon addition of sulfite. Mechanistic studies indicate that three probe-SO2 adducts coexist after reaction, as supported by liquid chromatography and density function theory investigations. Importantly, the ratiometric probe is highly selective for sulfite over other bio-species including H2S. Fluorescence co-localization studies indicate that the probe localizes solely in the mitochondria of HeLa cells. Last but not least, fluorescent imaging of HeLa cells successfully demonstrates the detection of intrinsically generated intracellular SO2 derivatives in living cells.
- Published
- 2015
34. Synthesis and Systematic Evaluation of Dark Resonance Energy Transfer (DRET)-Based Library and Its Application in Cell Imaging
- Author
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Nam-Young Kang, Chai Lean Teoh, Xiaotong Yu, Dongdong Su, Srikanta Sahu, and Young-Tae Chang
- Subjects
Boron Compounds ,Fluorescence-lifetime imaging microscopy ,Molecular Structure ,business.industry ,Chemistry ,Organic Chemistry ,Quantum yield ,General Chemistry ,Biochemistry ,Acceptor ,Fluorescence ,Small Molecule Libraries ,symbols.namesake ,chemistry.chemical_compound ,Energy Transfer ,Cell Tracking ,Quantum dot ,Excited state ,Stokes shift ,symbols ,Optoelectronics ,BODIPY ,Coloring Agents ,business - Abstract
In this paper, we report a new strategy for constructing a dye library with large Stokes shifts. By coupling a dark donor with BODIPY acceptors of tunable high quantum yield, a novel dark resonance energy transfer (DRET)-based library, named BNM, has been synthesized. Upon excitation of the dark donor (BDN) at 490 nm, the absorbed energy is transferred to the acceptor (BDM) with high efficiency, which was tunable in a broad range from 557 nm to 716 nm, with a high quantum yield of up to 0.8. It is noteworthy to mention that the majority of the non-radiative energy loss of the donor was converted into the acceptor's fluorescence output with a minimum leak of donor emission. Fluorescence imaging tested in live cells showed that the BNM compounds are cell-permeable and can also be employed for live-cell imaging. This is a new library which can be excited through a dark donor allowing for strong fluorescence emission in a wide range of wavelengths. Thus, the BNM library is well suited for high-throughput screening or multiplex experiments in biological applications by using a single laser excitation source.
- Published
- 2014
35. Development of a BODIPY-based fluorescent probe for imaging pathological tau aggregates in live cells
- Author
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Sungsu Lim, Yun Kyung Kim, Jun-Seok Lee, Dongdong Su, Dohee Kim, Young-Tae Chang, and Md. Mamunul Haque
- Subjects
Boron Compounds ,Nanotechnology ,Mice, Transgenic ,tau Proteins ,010402 general chemistry ,01 natural sciences ,Catalysis ,Cell Line ,chemistry.chemical_compound ,Mice ,Protein Aggregates ,mental disorders ,Materials Chemistry ,Animals ,Humans ,Fluorescent Dyes ,Neurons ,Molecular Structure ,010405 organic chemistry ,Chemistry ,Optical Imaging ,Metals and Alloys ,General Chemistry ,Fluorescence ,0104 chemical sciences ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Disease Models, Animal ,Ceramics and Composites ,Biophysics ,BODIPY - Abstract
Neuronal accumulation of tau aggregates is a pathological hallmark in multiple neurodegenerative disorders, collectively called tauopathies. A tau aggregation sensor that can monitor abnormal tau aggregation in neurons would facilitate the study of tau aggregation processes and the discovery of tau aggregation blockers. Here, we describe a BODIPY-fluorescence sensor (BD-tau) that selectively responds to pathological tau aggregates in live cells.
- Published
- 2017
36. Dark to light! A new strategy for large Stokes shift dyes: coupling of a dark donor with tunable high quantum yield acceptors
- Author
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Juwon Oh, Sung-Chan Lee, Jong Min Lim, Dongdong Su, Xiaotong Yu, Dongho Kim, Srikanta Sahu, and Young-Tae Chang
- Subjects
Chemistry ,Astrophysics::High Energy Astrophysical Phenomena ,Quantum yield ,Resonance ,General Chemistry ,Photochemistry ,Fluorescence ,Acceptor ,symbols.namesake ,chemistry.chemical_compound ,Förster resonance energy transfer ,Stokes shift ,Excited state ,symbols ,Physics::Chemical Physics ,BODIPY - Abstract
A new strategy for constructing large Stokes shift dyes by coupling a low quantum yield (less than 1%) BODIPY donor (BDN) with tunable high quantum yield BODIPY acceptors (BDM) has been explored to synthesize a set of novel Dark Resonance Energy Transfer (DRET) dyes, named BNM. The low quantum yield of the donor is ascribed to the intramolecular rotation of the phenyl rings, which has been proven by controlling the viscosity and temperature of the solvent. However, upon excitation of BNM compounds at the donor absorption wavelength, tunable emissions from 560 nm to 617 nm were obtained, with a high quantum yield of up to 0.75. Ultrafast dynamic studies demonstrated that the absorbed energy was transferred to the acceptor (BDM) with a high energy transfer rate, before being quenched by non-radiative intramolecular rotations. Using a dark donor makes it possible to avoid fluorescence leaks from donor emission. This is a new set of RET dyes that can be excited by a low quantum yield donor to emit a tunable wide range of high fluorescence emission.
- Published
- 2014
37. Boronic Acid: A Bio-Inspired Strategy To Increase the Sensitivity and Selectivity of Fluorescent NADH Probe
- Author
-
Yong Ni, Jungyeol Lee, Lin Yuan, Juanjuan Peng, Stuart N. Berry, Young-Tae Chang, Dongdong Su, Jingye Zhang, Beomsue Kim, and Lu Wang
- Subjects
010402 general chemistry ,01 natural sciences ,Biochemistry ,Catalysis ,chemistry.chemical_compound ,Colloid and Surface Chemistry ,Molecular recognition ,Live cell imaging ,Biomimetics ,Limit of Detection ,Cell Line, Tumor ,Humans ,Fluorescent Dyes ,Detection limit ,chemistry.chemical_classification ,010405 organic chemistry ,Chemistry ,Biomolecule ,General Chemistry ,Hydrogen-Ion Concentration ,NAD ,Fluorescence ,Combinatorial chemistry ,Boronic Acids ,0104 chemical sciences ,NAD+ kinase ,Selectivity ,Boronic acid - Abstract
Fluorescent probes have emerged as an essential tool in the molecular recognition events in biological systems; however, due to the complex structures of certain biomolecules, it remains a challenge to design small-molecule fluorescent probes with high sensitivity and selectivity. Inspired by the enzyme-catalyzed reaction between biomolecule and probe, we present a novel combination-reaction two-step sensing strategy to improve sensitivity and selectivity. Based on this strategy, we successfully prepared a turn-on fluorescent reduced nicotinamide adenine dinucleotide (NADH) probe, in which boronic acid was introduced to bind with NADH and subsequently accelerate the sensing process. This probe shows remarkably improved sensitivity (detection limit: 0.084 μM) and selectivity to NADH in the absence of any enzymes. In order to improve the practicality, the boronic acid was further modified to change the measurement conditions from alkalescent (pH 9.5) to physiological environment (pH 7.4). Utilizing these probes, we not only accurately quantified the NADH weight in a health care product but also evaluated intracellular NADH levels in live cell imaging. Thus, these bio-inspired fluorescent probes offer excellent tools for elucidating the roles of NADH in biological systems as well as a practical strategy to develop future sensitive and selective probes for complicated biomolecules.
- Published
- 2016
38. A Simple BODIPY-Based Viscosity Probe for Imaging of Cellular Viscosity in Live Cells
- Author
-
Nengyue Gao, Qing-Hua Xu, Young-Tae Chang, Dongdong Su, and Chai Lean Teoh
- Subjects
Boron Compounds ,Fluorescence-lifetime imaging microscopy ,Work (thermodynamics) ,FLIM ,Cell Survival ,Analytical chemistry ,02 engineering and technology ,010402 general chemistry ,sensors ,lcsh:Chemical technology ,01 natural sciences ,Biochemistry ,Article ,Analytical Chemistry ,BODIPY ,fluorescent probes ,viscosity ,cell imaging ,Viscosity ,chemistry.chemical_compound ,Humans ,lcsh:TP1-1185 ,Electrical and Electronic Engineering ,Instrumentation ,Chemistry ,Optical Imaging ,021001 nanoscience & nanotechnology ,Fluorescence ,Atomic and Molecular Physics, and Optics ,0104 chemical sciences ,Solvent ,Coupling (electronics) ,Fluorescence intensity ,Spectrometry, Fluorescence ,Microscopy, Fluorescence ,Molecular Probes ,Biophysics ,Solvents ,0210 nano-technology ,HeLa Cells - Abstract
Intracellular viscosity is a fundamental physical parameter that indicates the functioning of cells. In this work, we developed a simple boron-dipyrromethene (BODIPY)-based probe, BTV, for cellular mitochondria viscosity imaging by coupling a simple BODIPY rotor with a mitochondria-targeting unit. The BTV exhibited a significant fluorescence intensity enhancement of more than 100-fold as the solvent viscosity increased. Also, the probe showed a direct linear relationship between the fluorescence lifetime and the media viscosity, which makes it possible to trace the change of the medium viscosity. Furthermore, it was demonstrated that BTV could achieve practical applicability in the monitoring of mitochondrial viscosity changes in live cells through fluorescence lifetime imaging microscopy (FLIM).
- Published
- 2016
39. Development of background-free tame fluorescent probes for intracellular live cell imaging
- Author
-
Lingna Hu, Enrique Alvarado-Martínez, Young-Tae Chang, Hee-Sung Park, Rudrakanta Satapathy, Christian Hoppmann, Jihyo Kim, Eduardo Peña-Cabrera, Lei Wang, Haiyan Ren, Samira Husen Alamudi, Dongdong Su, Raj Kumar Das, Jung Yeol Lee, and Hyung-Ho Ha
- Subjects
Gene Expression ,Golgi Apparatus ,General Physics and Astronomy ,Signal-To-Noise Ratio ,01 natural sciences ,Cyclooctanes ,chemistry.chemical_compound ,chemistry.chemical_classification ,Tumor ,Multidisciplinary ,Fluorescence ,Recombinant Proteins ,Mitochondria ,Molecular Imaging ,3. Good health ,Cell biology ,Cheminformatics ,BODIPY ,Cell Nucleolus ,Intracellular ,Biotechnology ,Boron Compounds ,Azides ,Science ,Bioengineering ,CHO Cells ,Biology ,010402 general chemistry ,Article ,General Biochemistry, Genetics and Molecular Biology ,Cell Line ,Cricetulus ,Live cell imaging ,Cell Line, Tumor ,Animals ,Humans ,Fluorescent Dyes ,Osteoblasts ,Staining and Labeling ,010405 organic chemistry ,Biomolecule ,Rational design ,General Chemistry ,High-Throughput Screening Assays ,0104 chemical sciences ,chemistry ,Drug Design ,Molecular imaging ,Lysosomes - Abstract
Fluorescence labelling of an intracellular biomolecule in native living cells is a powerful strategy to achieve in-depth understanding of the biomolecule's roles and functions. Besides being nontoxic and specific, desirable labelling probes should be highly cell permeable without nonspecific interactions with other cellular components to warrant high signal-to-noise ratio. While it is critical, rational design for such probes is tricky. Here we report the first predictive model for cell permeable background-free probe development through optimized lipophilicity, water solubility and charged van der Waals surface area. The model was developed by utilizing high-throughput screening in combination with cheminformatics. We demonstrate its reliability by developing CO-1 and AzG-1, a cyclooctyne- and azide-containing BODIPY probe, respectively, which specifically label intracellular target organelles and engineered proteins with minimum background. The results provide an efficient strategy for development of background-free probes, referred to as ‘tame' probes, and novel tools for live cell intracellular imaging., The success of a fluorescent dye as a molecular probe to monitor the intracellular activity of biomolecules depends on its physicochemical characteristics. Here, the authors use a predictive model to identify key features that allow them to design cell permeable, background-free fluorescent probes.
- Published
- 2016
40. Ferrocene-based imidazolium receptors for anions
- Author
-
Dongdong Su, Hao-Tao Niu, Yinbatu Ao, Dong Niu, Zhenming Yin, Jiaqi He, and Jin-Pei Cheng
- Subjects
Chemistry ,Hydrogen bond ,Organic Chemistry ,Inorganic chemistry ,Electrochemistry ,Biochemistry ,Electrochemical gas sensor ,Crystallography ,chemistry.chemical_compound ,Ferrocene ,Drug Discovery ,Proton NMR ,Titration ,Receptor ,Selectivity - Abstract
Cyclic and acyclic ferrocene derivatives bearing two imidazolium rings have been synthesized and characterized by NMR, elemental analysis, mass spectra, and X-ray crystallography. Electrochemical measurements revealed that all the receptors displayed a significant anodic shift response for F−. In addition, for receptors 1, 2, and 4, addition of HSO4− induced quite different electrochemical behavior with dramatic cathodic peak current increase on CV. 1H NMR titrations demonstrated that receptors 1, 2, and 4 showed selectivity for AcO− while receptor 3 exhibited high affinity toward Cl− among the anions investigated.
- Published
- 2008
41. The development of a highly photostable and chemically stable zwitterionic near-infrared dye for imaging applications
- Author
-
Dongdong Su, Chai Lean Teoh, Sungjin Park, Young-Tae Chang, Nam-Young Kang, and Animesh Samanta
- Subjects
Infrared ,Cell Survival ,Infrared Rays ,Photochemistry ,Peptides, Cyclic ,Catalysis ,Drug Stability ,Live cell imaging ,Materials Chemistry ,Organic chemistry ,Humans ,Cell survival ,Fluorescent Dyes ,Chemistry ,Near-infrared spectroscopy ,technology, industry, and agriculture ,Metals and Alloys ,General Chemistry ,Chromophore ,equipment and supplies ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,Molecular Imaging ,Drug Design ,Ceramics and Composites ,MCF-7 Cells ,Chemical stability - Abstract
A novel zwitterionic near-infrared (NIR) dye, ZWCC, has been designed and synthesized. It shows significantly enhanced photostability and chemical stability compared to the existing zwitterionic NIR dye. In addition, the feasibility of labeling ZWCC with biological ligands was investigated and used in live cell imaging applications.
- Published
- 2015
42. Application of synergistic β-lactamase inhibitors and antibiotics in the treatment of wounds infected by superbugs
- Author
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Xiaoyuan Ding, Jing Su, Xiao Chen, Shizhong Zhang, Mengkao Li, Jie yang, Zekun Wang, Jingyuan Wang, Weiyun Wang, Dongdong Sun, and Guojun Wang
- Subjects
Prussian blue ,β-lactamase inhibitor ,Amoxicillin ,Antibacterial action ,Antibiotic resistance ,Chemistry ,QD1-999 - Abstract
Amoxicillin appears to be clinically drug-resistant due to the presence of β-lactamase in bacteria. Here, we designed and prepared a hollow Prussian Blue (HPB)-based therapeutic nanoplatform that was constructed by encapsulating amoxicillin into polyethyleneimine with β-lactamase inhibitor 4-carboxyphenylboronic acid (4-Cpba) decorated HPB nanoparticles (CPA NPs). The antibacterial effect of the CPA NPs on drug-resistant bacteria was observed by in vitro colony-forming unit, minimum inhibitory concentration, scanning electron microscopy, and fluorescence tests. The results show that amoxicillin effectively inhibited Escherichia coli and Staphylococcus aureus-resistant bacteria in the presence of 4-Cpba. The in vivo experimental results show that the CPA NPs exhibited a synergistic anti-infective effect in vivo, which inhibited the inflammatory response and apoptosis induced by the drug-resistant bacterial infection, and promoted wound healing in mice. The hematoxylin and eosin staining and blood biochemical experiments revealed that the acute toxicity of the material was negligible and it had good biocompatibility. Our results verify our design that CPA NPs can restore the antibacterial activity of amoxicillin.
- Published
- 2022
- Full Text
- View/download PDF
43. Sensors: Development of a Highly Selective, Sensitive, and Fast Response Upconversion Luminescent Platform for Hydrogen Sulfide Detection (Adv. Funct. Mater. 2/2016)
- Author
-
Chai Lean Teoh, Lu Wang, Xiaogang Liu, Animesh Samanta, Dongdong Su, Wang Xu, Xiao Zeng, Lin Yuan, Juanjuan Peng, and Young-Tae Chang
- Subjects
Materials science ,Hydrogen sulfide ,Nanotechnology ,02 engineering and technology ,010402 general chemistry ,021001 nanoscience & nanotechnology ,Condensed Matter Physics ,Highly selective ,01 natural sciences ,Photon upconversion ,0104 chemical sciences ,Electronic, Optical and Magnetic Materials ,Biomaterials ,chemistry.chemical_compound ,chemistry ,Electrochemistry ,0210 nano-technology ,Luminescence - Published
- 2016
44. Imidazolium-based macrocycles as multisignaling chemosensors for anions
- Author
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Jin-Pei Cheng, Jiaqi He, Hao-Tao Niu, Zhenming Yin, Dong Niu, and Dongdong Su
- Subjects
Anions ,Macrocyclic Compounds ,Magnetic Resonance Spectroscopy ,Metallocenes ,Inorganic chemistry ,Naphthalenes ,Crystallography, X-Ray ,Electrochemistry ,Medicinal chemistry ,Chemistry Techniques, Analytical ,Fluorescence ,Inorganic Chemistry ,chemistry.chemical_compound ,Quinoxalines ,Moiety ,Ferrous Compounds ,Anion binding ,Acetonitrile ,Anthracenes ,Binding Sites ,Chemistry ,Imidazoles ,Titrimetry ,Ferrocene ,Proton NMR ,Spectrophotometry, Ultraviolet ,Selectivity - Abstract
A series of structurally novel anion receptors , , and in which a ferrocene unit and a fluorescent moiety are linked to two imidazolium rings have been designed and prepared from 1,1'-bis(imidazolylmethyl)ferrocene. Their crystal structures revealed that these receptors are capable of incorporating anions such as PF(6)(-) and Br(-). Consequently, the anion binding studies were carried out using various techniques including electrochemistry (CV and OSWV), fluorescence, UV-vis, and (1)H NMR spectroscopy. All the receptors showed a special electrochemical response to the F(-) anion with a remarkable cathodic shift of more than 260 mV and displayed a unique selectivity for F(-) and AcO(-) anions with fluorescence enhancement over various other anions of present interest (Cl(-), Br(-), I(-), HSO(4)(-), H(2)PO(4)(-)). In addition, for receptor , obvious absorption changes were observed when the H(2)PO(4)(-) anion was added while other anions (F(-), Cl(-), Br(-), I(-), AcO(-), HSO(4)(-)) showed only a minor influence on the UV-vis spectra. (1)H NMR titrations demonstrated that receptors and can bind anions through (C-H)(+)X(-) hydrogen bonds and showed strong affinity and high selectivity for the AcO(-) anion in acetonitrile.
- Published
- 2008
45. A simple yet highly selective colorimetric sensor for cyanide anion in an aqueous environment
- Author
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Hao-Tao Niu, Jin-Pei Cheng, Zhenming Yin, Jiaqi He, Wenzhi Yang, Xueliang Jiang, and Dongdong Su
- Subjects
Anions ,Models, Molecular ,Cyanides ,Aqueous solution ,Molecular Structure ,Cyanide ,Organic Chemistry ,Inorganic chemistry ,Anthraquinones ,Biosensing Techniques ,Highly selective ,Biochemistry ,Anthraquinone ,Colorimetry (chemical method) ,chemistry.chemical_compound ,Colorimetric sensor ,chemistry ,Nucleophile ,Molecular Probes ,Colorimetry ,Physical and Theoretical Chemistry ,Cyanide anion - Abstract
The nucleophilic nature of cyanide is used to create a simple, sensitive, and highly effective sensor, 2-(trifluoroacetylamino)anthraquinone (2-TFAQ), for the easy "naked-eye" detection of very low concentrations of cyanide in an aqueous environment.
- Published
- 2008
46. An ESIPT‐Based Ratiometric Fluorescent Probe for Highly Sensitive and Rapid Detection of Sulfite in Living Cells
- Author
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Yupeng Liu, Dr. Tian‐Bing Ren, Dr. Dan Cheng, Jianing Hou, Prof. Dongdong Su, and Prof. Lin Yuan
- Subjects
fluorescent probe ,sulfite ,ratiometric probes ,fluorescence ,imaging studies ,Chemistry ,QD1-999 - Abstract
Abstract The novel ratiometric fluorescent probe HPQRB with an ESIPT effect based on Michael addition for highly sensitive and fast detection of sulfite in living HepG2 cells is reported. HPQRB can be easily synthesized by a two‐step condensation reaction. HPQRB has a large emission shift (Δλ=116 nm), which is beneficial for fluorescence imaging research, and its sulfite‐responsive site is based on a rhodamine‐like structure with the emission peak at 566 nm, which decreases with increasing sulfite concentration. and its HPQ structure always has an ESIPT effect throughout the reaction process, keeping the emission peak at 450 nm as a self‐reference. In particular, HPQRB has high selectivity for sulfite and responds quickly (within 30 s) with a low detection limit (44 nM). Furthermore, HPQRB has been successfully used for fluorescence imaging of sulfite in HepG2 cells, demonstrating the superior ability to detect sulfite under physiological conditions.
- Published
- 2019
- Full Text
- View/download PDF
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