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Visualizing Microglia with a Fluorescence TurnāOn Ugt1a7c Substrate
- Source :
- Angewandte Chemie International Edition. 58:7972-7976
- Publication Year :
- 2019
- Publisher :
- Wiley, 2019.
-
Abstract
- Microglia, the brain-resident macrophage, are involved in brain development and contribute to the progression of neural disorders. Despite the importance of microglia, imaging of live microglia at a cellular resolution has been limited to transgenic mice. Efforts have therefore been dedicated to developing new methods for microglia detection and imaging. Using a thorough structure-activity relationships study, we developed CDr20, a high-performance fluorogenic chemical probe that enables the visualization of microglia both in vitro and in vivo. Using a genome-scale CRISPR-Cas9 knockout screen, the UDP-glucuronosyltransferase Ugt1a7c was identified as the target of CDr20. The glucuronidation of CDr20 by Ugt1a7c in microglia produces fluorescence.
- Subjects :
- Genetically modified mouse
Brain development
Microglia
010405 organic chemistry
Chemistry
Optical Imaging
Chemical probe
General Chemistry
010402 general chemistry
01 natural sciences
Fluorescence
Catalysis
In vitro
0104 chemical sciences
Mice
medicine.anatomical_structure
nervous system
In vivo
medicine
Animals
Macrophage
Glucuronosyltransferase
Neuroscience
Fluorescent Dyes
Subjects
Details
- ISSN :
- 15213773 and 14337851
- Volume :
- 58
- Database :
- OpenAIRE
- Journal :
- Angewandte Chemie International Edition
- Accession number :
- edsair.doi.dedup.....0caa8f99bee845f8ee47d61a1349ff94