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A mitochondria-targeted ratiometric fluorescent probe to monitor endogenously generated sulfur dioxide derivatives in living cells
- Source :
- Biomaterials. 56:1-9
- Publication Year :
- 2015
- Publisher :
- Elsevier BV, 2015.
-
Abstract
- Sulfur dioxide (SO2) can be endogenously produced by enzymes in mitochondria during oxidation of H2S or sulphur-containing amino acids, and plays important roles in several physiological processes. However, the design and synthesis of fluorescent probes which can detect mitochondrial SO2 and its derivatives in living cells still remain unresolved. Herein, we report the preparation of a lipophilic cationic dye 1 (Mito-Ratio-SO2), which targets the mitochondria in living cells and is sensitive to the presence of SO2 derivatives. The ratiometric probe Mito-Ratio-SO2 displays a 170 nm blue-shift in emission with two well-resolved emission bands upon addition of sulfite. Mechanistic studies indicate that three probe-SO2 adducts coexist after reaction, as supported by liquid chromatography and density function theory investigations. Importantly, the ratiometric probe is highly selective for sulfite over other bio-species including H2S. Fluorescence co-localization studies indicate that the probe localizes solely in the mitochondria of HeLa cells. Last but not least, fluorescent imaging of HeLa cells successfully demonstrates the detection of intrinsically generated intracellular SO2 derivatives in living cells.
- Subjects :
- Indoles
Biophysics
Bioengineering
Biosensing Techniques
Mitochondrion
complex mixtures
Adduct
Biomaterials
HeLa
chemistry.chemical_compound
Sulfite
Coumarins
Cations
Humans
Sulfites
Sulfur Dioxide
Hydrogen Sulfide
Fluorescent Dyes
chemistry.chemical_classification
biology
biology.organism_classification
Fluorescence
Mitochondria
respiratory tract diseases
Amino acid
Enzyme
Microscopy, Fluorescence
chemistry
Biochemistry
Mechanics of Materials
Ceramics and Composites
Intracellular
HeLa Cells
Subjects
Details
- ISSN :
- 01429612
- Volume :
- 56
- Database :
- OpenAIRE
- Journal :
- Biomaterials
- Accession number :
- edsair.doi.dedup.....6e60971eb6378edef2882aa7fa0113e0