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Visualization of oxidative injury in the mouse kidney using selective superoxide anion fluorescent probes† †Electronic supplementary information (ESI) available: Experimental procedures, characterization data, and additional spectra. See DOI: 10.1039/c8sc03308k
- Source :
- Chemical Science
- Publication Year :
- 2018
- Publisher :
- Royal Society of Chemistry, 2018.
-
Abstract
- Drug-induced acute kidney injury (AKI), caused by renal drug metabolism, has been regarded as a main problem in clinical pharmacology and practice.<br />Drug-induced acute kidney injury (AKI), caused by renal drug metabolism, has been regarded as a main problem in clinical pharmacology and practice. However, due to the lack of effective biomarkers and noninvasive real-time tools, the early diagnosis of drug-induced AKI is still a crucial challenge. The superoxide anion (O2˙–), the preliminary reactive oxidative species, is closely related to drug-induced AKI. In this paper, we reported two new mitochondria-targeted fluorescent probes for investigating AKI via mapping the fluctuation of O2˙– with high sensitivity and selectivity by the combination of rational design and a probe-screening approach. Small-molecule fluorescent probes (Naph-O2˙– and NIR-O2˙–) with high accuracy and excellent selectivity were successfully applied to detect endogenously produced O2˙– in living cells and tissues by dual-model confocal imaging, and to trap the fluctuation of the O2˙– level during the drug-induced nephrotoxicity. Moreover, probe NIR-O2˙– was also used to elucidate the protective effects of l-carnitine (LC) against drug-induced nephrotoxicity for the first time. Therefore, these probes may be potential chemical tools for exploring the roles of O2˙– in complex nephrotoxicity disease systems.
Details
- Language :
- English
- ISSN :
- 20416539 and 20416520
- Volume :
- 9
- Issue :
- 39
- Database :
- OpenAIRE
- Journal :
- Chemical Science
- Accession number :
- edsair.pmid..........c28f14a2a111605011c89bb0d22a2e74